ELECTROPHORESIS最新文献

Contents: Electrophoresis 7–8'25 内容：电泳7-8′25

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-29 DOI: 10.1002/elps.202570042

引用次数: 0

Editorial Board: Electrophoresis 7–8'25 编辑委员会：电泳7-8 '25

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-29 DOI: 10.1002/elps.202570041

引用次数: 0

Characterization of Taurocholic Acid Binding With Insulin for Potential Oral Formulation Using Different Methods 不同方法表征牛磺胆酸与潜在口服制剂胰岛素的结合

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-16 DOI: 10.1002/elps.8139

Chang Sun, Shuanghao Wang, Huihui Li, David Da Yong Chen

{"title":"Characterization of Taurocholic Acid Binding With Insulin for Potential Oral Formulation Using Different Methods","authors":"Chang Sun, Shuanghao Wang, Huihui Li, David Da Yong Chen","doi":"10.1002/elps.8139","DOIUrl":"https://doi.org/10.1002/elps.8139","url":null,"abstract":"In diabetes management, oral formulation of insulin (INS) has the potential to improve safety, convenience, and patient-centered care compared to subcutaneous injections. However, its bioavailability remains limited, necessitating improved delivery strategies. Recent clinical trials indicate that taurocholic acid (TCA) can enhance the bioavailability of oral INS as an absorption enhancer. In this work, electrospray ionization mass spectrometry (ESI-MS) analysis revealed the formation of 1:1–1:4 INS–TCA complexes. MS/MS was used to explore the fragmentation pathway of complex ions and confirm binding stability in the gas phase. Circular dichroism spectra showed no clear conformational change in INS upon TCA binding, even though TCA enhanced INS's structural stability. Using Taylor dispersion analysis (TDA), we determined the diffusion coefficient and hydrodynamic radius of INS and its complexes. TCA binding was observed to increase INS size in both the 1:1 and 1:2 INS–TCA complexes. The binding constant of INS and TCA (1.3 × 103 L/mol) with approximately five binding sites was obtained via pressure-assisted capillary electrophoresis frontal analysis. Molecular docking simulations indicated that TCA binds to external binding sites on the INS B chain (near Ser-B9, Glu-B13, and Phe-B24 residues), consistent with ESI-MS and TDA results. These findings suggest that TCA binding may enhance INS absorption and increase the bioavailability of oral INS therapy.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 7-8","pages":"468-477"},"PeriodicalIF":3.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.8139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postulations for the Migration Behavior of Amino Acids as Cations in Capillary Zone Electrophoresis. 毛细管区带电泳中氨基酸作为阳离子迁移行为的假设。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-09 DOI: 10.1002/elps.202400205

Peter Gross, Tom Huber, Isabel Lunow, Dominik Burkhard, Holger Seelert, Rolf Müller

{"title":"Postulations for the Migration Behavior of Amino Acids as Cations in Capillary Zone Electrophoresis.","authors":"Peter Gross, Tom Huber, Isabel Lunow, Dominik Burkhard, Holger Seelert, Rolf Müller","doi":"10.1002/elps.202400205","DOIUrl":"https://doi.org/10.1002/elps.202400205","url":null,"abstract":"Amino acids (AAs) in their cationic form at pH 2.2 and usual ionic strength show a non-intuitive migration order in CZE. This is explained by setting up four postulates. The central points in these postulates are the influence of the AA side chain on the <math> <semantics><mrow><mi>p</mi> <msub><mi>K</mi> <mi>a</mi></msub> </mrow> <annotation>${rm p}K_{rm a}$</annotation></semantics> </math> value and the adoption of a defined, preferred conformation to build up the different <math> <semantics><mrow><mi>p</mi> <msub><mi>K</mi> <mi>a</mi></msub> </mrow> <annotation>${rm p}K_{rm a}$</annotation></semantics> </math> values. This conformation then also influences the hydrodynamic radius. The rotational orientation of an AA in the electric field aligns it, which also affects the hydrodynamic radius. Overall a special electrophoretical hydrodynamic radius is postulated and distinguished from the hydrodynamic radius, which is determined by the translational diffusion constant. With the help of the four postulates, the migration order could be explained. Glutamic acid has a special feature in this study: due to its observed higher mobility than the smaller and even higher charged aspartic acid, the hypothesis is that it would deprotonate first at the C5 and not at the C1 carboxylic group as all other AAs. This has the consequence of a more streamlined conformation and by that a faster migration in capillary electrophoresis.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Separation of Extracellular Vesicles Using Capillary Isotachophoresis With Spacer Compounds. 间隔化合物毛细管等速电泳强化细胞外囊泡分离。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-06 DOI: 10.1002/elps.202400113

Milan Pieter Paul de Putter, Andrea Capuano, Meia Numan, Thomas Hankemeier, Yuliya Shakalisava

{"title":"Enhanced Separation of Extracellular Vesicles Using Capillary Isotachophoresis With Spacer Compounds.","authors":"Milan Pieter Paul de Putter, Andrea Capuano, Meia Numan, Thomas Hankemeier, Yuliya Shakalisava","doi":"10.1002/elps.202400113","DOIUrl":"https://doi.org/10.1002/elps.202400113","url":null,"abstract":"Extracellular vesicles (EVs) are pivotal in numerous physiological and pathological processes, such as immune responses, viral pathogenesis, pregnancy, cardiovascular diseases, and cancer progression. Their capacity to influence complex intracellular pathways highlights their therapeutic potential in addressing various conditions, including neurodegenerative diseases and cancer. A novel capillary isotachophoresis (cITP) method was developed for the electrokinetic characterization of pre-isolated EVs. Distinct peaks could be resolved at near-baseline resolution using a novel mixture of spacer ions and laser-induced fluorescence (LIF) detection. The vesicles were effectively separated from the unbound carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) amine-reactive fluorescent stain used to detect them and from residual contaminants. The identity of the peaks shown in the electropherograms was validated via various methods, including incubation with specific antibodies or spiking of putative contaminants, such as proteins and lipoproteins. This report thus provides a detailed proof-of-concept for using cITP-LIF for extracellular vesicle isolation, subtype fractionation, and profiling.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CFD-Based Optimization of a Dielectrophoretic Device to Isolate CTCs 基于cfd的ctc分离介质电泳装置优化。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-04 DOI: 10.1002/elps.8129

Lan Qin, Xiao Liu, Fei Fei

引用次数: 0

Editorial Board: Electrophoresis 5–6'25 编委会：电泳5-6 '25

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-28 DOI: 10.1002/elps.202570031

引用次数: 0

Contents: Electrophoresis 5–6'25 内容：电泳5-6′25

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-28 DOI: 10.1002/elps.202570032

引用次数: 0

Open-Tubular Affinity Capillary Electrochromatography Based on Immobilized Receptor by Pore Entrapment With Metal–Organic Frameworks 基于金属-有机框架孔包埋固定受体的开管亲和毛细管电色谱。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-27 DOI: 10.1002/elps.8117

Meiling Zhou, Min Wang, Hanwen Deng, Xiaoyu Chen, Min Wang, Yike Huang, Zhining Xia

{"title":"Open-Tubular Affinity Capillary Electrochromatography Based on Immobilized Receptor by Pore Entrapment With Metal–Organic Frameworks","authors":"Meiling Zhou, Min Wang, Hanwen Deng, Xiaoyu Chen, Min Wang, Yike Huang, Zhining Xia","doi":"10.1002/elps.8117","DOIUrl":"10.1002/elps.8117","url":null,"abstract":"<div>\u0000 \u0000 This paper introduces a novel preparation of open-tubular affinity capillary electrochromatography (OT-ACEC) columns through immobilizing proteins by pore entrapment with metal-organic frameworks (MOFs) to rapidly investigate drug–protein interactions. The MOFs material PCN-333(Al) was used to immobilize the model receptor bovine hemoglobin (BHb) for preparing BHb@MOFs, and the affinity chromatography BHb@MOFs/PDA@capillary was created through the immobilization of BHb@MOFs facilitated by polydopamine (PDA). The disappearance of the mesoporous pore of PCN-333 (Al) proved the encapsulation of BHb within the pore of PCN-333(Al). The BHb@MOFs/PDA@capillary column exhibited a good affinity screening and separation ability to distinguish flavonoids and sulfonamides. Besides, the normalized capacity factor (KIRCE) was used to evaluate drug–protein interactions and related to apparent migration times and the amount of immobilized protein. The binding constant (Ka) values was derived from fluorescence quenching analysis, and a robust positive correlation was observed between KIRCE and lg Ka (R = 0.94783, p < 0.00001). These results indicate that KIRCE can be used as an effective index to evaluate the affinity between protein molecules and drug molecules and the OT-ACEC has the potential to predict drug activity.\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 7-8","pages":"357-364"},"PeriodicalIF":3.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A SNaPshot Assay for Epigenetic Age Prediction of Costal Cartilage 用快照法预测肋软骨表观遗传年龄。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-27 DOI: 10.1002/elps.8132

Ju Yeon Jung, Yeon Woo Song, Kyu-Sik Jeong, Hyun-Chul Park, Moon Hyun So, Hwan Young Lee

{"title":"A SNaPshot Assay for Epigenetic Age Prediction of Costal Cartilage","authors":"Ju Yeon Jung, Yeon Woo Song, Kyu-Sik Jeong, Hyun-Chul Park, Moon Hyun So, Hwan Young Lee","doi":"10.1002/elps.8132","DOIUrl":"10.1002/elps.8132","url":null,"abstract":"Estimating age at death narrows the pool of potential donors in mass disasters and criminal investigations. In this study, we developed a capillary electrophoresis-based SNaPshot assay for age prediction of costal cartilage and used it to analyze DNA methylation at 11 CpG sites across six genes in 136 samples from deceased Koreans aged 28–84 years. To develop the predictive model, DNA methylation levels at these sites from a training set of 83 samples were analyzed using multivariate linear regression in five ways. We then compared the performance parameters calculated from the training set and a test set of 53 samples. Considering experimental simplicity, we selected a model that incorporates four CpGs (MIR29B2CHG_C2, FHL2_C4, TRIM59_C3, and KLF14_C3) as the optimal age prediction model, demonstrating high performance with a mean absolute error of 4.60 years and a root mean square error of 5.41 years in the test set. Subsequently, we developed a multiplex SNaPshot system covering CpGs included in the optimal model, requiring a minimum of 4 ng of bisulfite-converted DNA for reliable prediction and demonstrating multi-tissue applicability, particularly in blood and buccal swabs. We believe this tool will support forensic investigations, including the identification of victims and missing persons.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 7-8","pages":"413-423"},"PeriodicalIF":3.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.8132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0