ELECTROPHORESIS最新文献

Postulations for the Migration Behavior of Amino Acids as Cations in Capillary Zone Electrophoresis. 毛细管区带电泳中氨基酸作为阳离子迁移行为的假设。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-09 DOI: 10.1002/elps.202400205

Peter Gross, Tom Huber, Isabel Lunow, Dominik Burkhard, Holger Seelert, Rolf Müller

{"title":"Postulations for the Migration Behavior of Amino Acids as Cations in Capillary Zone Electrophoresis.","authors":"Peter Gross, Tom Huber, Isabel Lunow, Dominik Burkhard, Holger Seelert, Rolf Müller","doi":"10.1002/elps.202400205","DOIUrl":"https://doi.org/10.1002/elps.202400205","url":null,"abstract":"Amino acids (AAs) in their cationic form at pH 2.2 and usual ionic strength show a non-intuitive migration order in CZE. This is explained by setting up four postulates. The central points in these postulates are the influence of the AA side chain on the <math> <semantics><mrow><mi>p</mi> <msub><mi>K</mi> <mi>a</mi></msub> </mrow> <annotation>${rm p}K_{rm a}$</annotation></semantics> </math> value and the adoption of a defined, preferred conformation to build up the different <math> <semantics><mrow><mi>p</mi> <msub><mi>K</mi> <mi>a</mi></msub> </mrow> <annotation>${rm p}K_{rm a}$</annotation></semantics> </math> values. This conformation then also influences the hydrodynamic radius. The rotational orientation of an AA in the electric field aligns it, which also affects the hydrodynamic radius. Overall a special electrophoretical hydrodynamic radius is postulated and distinguished from the hydrodynamic radius, which is determined by the translational diffusion constant. With the help of the four postulates, the migration order could be explained. Glutamic acid has a special feature in this study: due to its observed higher mobility than the smaller and even higher charged aspartic acid, the hypothesis is that it would deprotonate first at the C5 and not at the C1 carboxylic group as all other AAs. This has the consequence of a more streamlined conformation and by that a faster migration in capillary electrophoresis.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Separation of Extracellular Vesicles Using Capillary Isotachophoresis With Spacer Compounds. 间隔化合物毛细管等速电泳强化细胞外囊泡分离。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-06 DOI: 10.1002/elps.202400113

Milan Pieter Paul de Putter, Andrea Capuano, Meia Numan, Thomas Hankemeier, Yuliya Shakalisava

{"title":"Enhanced Separation of Extracellular Vesicles Using Capillary Isotachophoresis With Spacer Compounds.","authors":"Milan Pieter Paul de Putter, Andrea Capuano, Meia Numan, Thomas Hankemeier, Yuliya Shakalisava","doi":"10.1002/elps.202400113","DOIUrl":"https://doi.org/10.1002/elps.202400113","url":null,"abstract":"Extracellular vesicles (EVs) are pivotal in numerous physiological and pathological processes, such as immune responses, viral pathogenesis, pregnancy, cardiovascular diseases, and cancer progression. Their capacity to influence complex intracellular pathways highlights their therapeutic potential in addressing various conditions, including neurodegenerative diseases and cancer. A novel capillary isotachophoresis (cITP) method was developed for the electrokinetic characterization of pre-isolated EVs. Distinct peaks could be resolved at near-baseline resolution using a novel mixture of spacer ions and laser-induced fluorescence (LIF) detection. The vesicles were effectively separated from the unbound carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) amine-reactive fluorescent stain used to detect them and from residual contaminants. The identity of the peaks shown in the electropherograms was validated via various methods, including incubation with specific antibodies or spiking of putative contaminants, such as proteins and lipoproteins. This report thus provides a detailed proof-of-concept for using cITP-LIF for extracellular vesicle isolation, subtype fractionation, and profiling.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CFD-Based Optimization of a Dielectrophoretic Device to Isolate CTCs. 基于cfd的ctc分离介质电泳装置优化。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-04 DOI: 10.1002/elps.8129

Lan Qin, Xiao Liu, Fei Fei

引用次数: 0

Editorial Board: Electrophoresis 5–6'25 编委会：电泳5-6 '25

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-28 DOI: 10.1002/elps.202570031

引用次数: 0

Contents: Electrophoresis 5–6'25 内容：电泳5-6′25

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-28 DOI: 10.1002/elps.202570032

引用次数: 0

Open-Tubular Affinity Capillary Electrochromatography Based on Immobilized Receptor by Pore Entrapment With Metal-Organic Frameworks. 基于金属-有机框架孔包埋固定受体的开管亲和毛细管电色谱。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-27 DOI: 10.1002/elps.8117

Meiling Zhou, Min Wang, Hanwen Deng, Xiaoyu Chen, Min Wang, Yike Huang, Zhining Xia

{"title":"Open-Tubular Affinity Capillary Electrochromatography Based on Immobilized Receptor by Pore Entrapment With Metal-Organic Frameworks.","authors":"Meiling Zhou, Min Wang, Hanwen Deng, Xiaoyu Chen, Min Wang, Yike Huang, Zhining Xia","doi":"10.1002/elps.8117","DOIUrl":"https://doi.org/10.1002/elps.8117","url":null,"abstract":"This paper introduces a novel preparation of open-tubular affinity capillary electrochromatography (OT-ACEC) columns through immobilizing proteins by pore entrapment with metal-organic frameworks (MOFs) to rapidly investigate drug-protein interactions. The MOFs material PCN-333(Al) was used to immobilize the model receptor bovine hemoglobin (BHb) for preparing BHb@MOFs, and the affinity chromatography BHb@MOFs/PDA@capillary was created through the immobilization of BHb@MOFs facilitated by polydopamine (PDA). The disappearance of the mesoporous pore of PCN-333 (Al) proved the encapsulation of BHb within the pore of PCN-333(Al). The BHb@MOFs/PDA@capillary column exhibited a good affinity screening and separation ability to distinguish flavonoids and sulfonamides. Besides, the normalized capacity factor (KIRCE) was used to evaluate drug-protein interactions and related to apparent migration times and the amount of immobilized protein. The binding constant (Ka) values was derived from fluorescence quenching analysis, and a robust positive correlation was observed between KIRCE and lg Ka (R = 0.94783, p < 0.00001). These results indicate that KIRCE can be used as an effective index to evaluate the affinity between protein molecules and drug molecules and the OT-ACEC has the potential to predict drug activity.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A SNaPshot Assay for Epigenetic Age Prediction of Costal Cartilage. 用快照法预测肋软骨表观遗传年龄。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-27 DOI: 10.1002/elps.8132

Ju Yeon Jung, Yeon Woo Song, Kyu-Sik Jeong, Hyun-Chul Park, Moon Hyun So, Hwan Young Lee

{"title":"A SNaPshot Assay for Epigenetic Age Prediction of Costal Cartilage.","authors":"Ju Yeon Jung, Yeon Woo Song, Kyu-Sik Jeong, Hyun-Chul Park, Moon Hyun So, Hwan Young Lee","doi":"10.1002/elps.8132","DOIUrl":"https://doi.org/10.1002/elps.8132","url":null,"abstract":"Estimating age at death narrows the pool of potential donors in mass disasters and criminal investigations. In this study, we developed a capillary electrophoresis-based SNaPshot assay for age prediction of costal cartilage and used it to analyze DNA methylation at 11 CpG sites across six genes in 136 samples from deceased Koreans aged 28-84 years. To develop the predictive model, DNA methylation levels at these sites from a training set of 83 samples were analyzed using multivariate linear regression in five ways. We then compared the performance parameters calculated from the training set and a test set of 53 samples. Considering experimental simplicity, we selected a model that incorporates four CpGs (MIR29B2CHG_C2, FHL2_C4, TRIM59_C3, and KLF14_C3) as the optimal age prediction model, demonstrating high performance with a mean absolute error of 4.60 years and a root mean square error of 5.41 years in the test set. Subsequently, we developed a multiplex SNaPshot system covering CpGs included in the optimal model, requiring a minimum of 4 ng of bisulfite-converted DNA for reliable prediction and demonstrating multi-tissue applicability, particularly in blood and buccal swabs. We believe this tool will support forensic investigations, including the identification of victims and missing persons.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Evaluation of Analytical Method Parameters Critical to the Reliable Assessment of Therapeutic mRNA Integrity by Capillary Gel Electrophoresis. 对毛细管凝胶电泳可靠评估治疗性mRNA完整性关键分析方法参数的综合评价。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-25 DOI: 10.1002/elps.8123

Jessica P Tran, Jun Gao, Casey Lansdell, Barry Lorbetskie, Michael J W Johnston, Lisheng Wang, Xuguang Li, Huixin Lu

{"title":"A Comprehensive Evaluation of Analytical Method Parameters Critical to the Reliable Assessment of Therapeutic mRNA Integrity by Capillary Gel Electrophoresis.","authors":"Jessica P Tran, Jun Gao, Casey Lansdell, Barry Lorbetskie, Michael J W Johnston, Lisheng Wang, Xuguang Li, Huixin Lu","doi":"10.1002/elps.8123","DOIUrl":"https://doi.org/10.1002/elps.8123","url":null,"abstract":"In recent years, messenger ribonucleic acid (mRNA)-lipid nanoparticle (LNP) biotherapeutics have demonstrated significant promise in disease treatment and prevention given their rapidly modifiable production processes and considerable capacity to adapt to complex or low-yielding proteins of interest. As a result, many products are currently being developed in this space. Critically, well-characterized and appropriately designed assays are required to monitor purity and integrity in order to maintain the efficacy and consistency of these novel products. Currently, capillary gel electrophoresis with laser-induced fluorescence (CGE-LIF) and ion-pair reversed-phase liquid chromatography (IP-RPLC) are techniques of choice for mRNA integrity analysis. However, most methods proposed for biotherapeutic analysis have been developed using naked mRNA without LNP components or proprietary buffer formulations, which can obscure undiscovered impurities or complex interactions between mRNA and the sample matrix. In this study, we addressed these methodological challenges by using a biotherapeutically relevant commercial mRNA-LNP sample (approx. 4200 b) to refine and optimize a customizable CGE-LIF method currently under consideration for mRNA-LNP biotherapeutic analysis. We systematically characterized how critical method parameters-such as denaturant type, concentration, and usage-and LNP disruption protocols can interfere with accurate mRNA integrity analysis in CGE-LIF and IP-RPLC. We found that optimal conditions for CGE-LIF assay sensitivity, variability, and resolution included sample precipitation by isopropanol, high urea concentrations, no formamide as a sample diluent, and high concentrations of dye. Finally, the advantages and disadvantages of both CGE-LIF and IP-RPLC are highlighted, and a discussion of key considerations when using or designing methods for mRNA integrity assessment is presented.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical Study of Falling Droplets in a Vertical Electric Field. 垂直电场中水滴下落的数值研究。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-25 DOI: 10.1002/elps.8136

Hailong Zhang, Tingting Zhang, Yuxin Lu, Qingzhen Yang, Hui Xing

{"title":"Numerical Study of Falling Droplets in a Vertical Electric Field.","authors":"Hailong Zhang, Tingting Zhang, Yuxin Lu, Qingzhen Yang, Hui Xing","doi":"10.1002/elps.8136","DOIUrl":"https://doi.org/10.1002/elps.8136","url":null,"abstract":"It is of fundamental importance to study and understand the behavior of falling droplets. External fields, such as electric and magnetic fields, are considered promising methods for controlling falling droplets, especially at small scales. Although some experimental and theoretical work on falling droplets in an electric field has been conducted, a fully numerical model for electrohydrodynamic falling droplets is still lacking. In this article, we proposed a phase field numerical model and studied the falling droplets in a vertical electric field. In particular, the influence of the electric field on the velocity and interfacial morphology of the falling droplets was investigated. It was found that the vertical electric field can elongate the droplet in the vertical direction and increase its falling velocity. With a weak or no electric field (the electrical capillary number CaE < 1.0), an indentation appears on the top of the falling droplet, and the droplet evolves into a bowl-like structure. In contrast, a strong electric field (CaE ≥ 1.0) causes a protrusion on the droplet, transforming it into a thumbtack-like shape. The numerical model and the obtained results can improve our understanding of falling droplets and highlight potential ways to regulate their behaviors.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Ultra-Short KYCDE Peptides Using Si_xN_y Nanopores. 利用六纳米孔检测超短KYCDE肽。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-03-19 DOI: 10.1002/elps.8122

Chaoming Gu, Kamruzzaman Joty, Matthew O'Donohue, Navod Thyashan, Lifang Hu, Moon J Kim, Sangyoup Lee, Min Jun Kim

{"title":"Detection of Ultra-Short KYCDE Peptides Using SixNy Nanopores.","authors":"Chaoming Gu, Kamruzzaman Joty, Matthew O'Donohue, Navod Thyashan, Lifang Hu, Moon J Kim, Sangyoup Lee, Min Jun Kim","doi":"10.1002/elps.8122","DOIUrl":"https://doi.org/10.1002/elps.8122","url":null,"abstract":"Detection of ultra-short peptides is one of the critical steps toward deeper understanding of proteins and the sequencing of amino acids using solid-state nanopores. The ability of solid-state nanopores to detect these ultra-short peptides can help us reveal their hydrodynamic state under different conditions like the concentrations and the external voltage, which may further guide the future development in this field for deeper investigation and possible improvement. In this study, we fabricate SixNy nanopores by CDB with various pore sizes and use them to detect ultra-short peptides comprised of five different amino acids. The peptide translocation events are extracted under various external voltages. Optimal experimental conditions such as the concentration of electrolytes and analytes, and the range of external voltage are investigated and compared. The statistical results based on volume exclusion analysis indicate that a significant portion of peptides exist in aggregation form. Due to the limitations of SixNy nanopores such as the thickness and the noise, most of the single peptide signals are masked under the baseline noise. In addition, the results show that peptide-pore interactions are dependent upon the diameter of the nanopore. Higher voltage may also influence the degree of peptide aggregations. This study serves to further comprehend the physical and chemical properties of peptides, find possible ways to improve the performance of solid-state nanopores in the area of protein and peptide detections, and indicate the potential improvements in solid-state nanopore-based peptide sequencing.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0