ELECTROPHORESIS最新文献

{"title":"Comparative Study on the Electrophoresis of Soft and Semisoft Nanoparticles.","authors":"Saurabh K Maurya, Santanu Saha, Partha P Gopmandal","doi":"10.1002/elps.8149","DOIUrl":"https://doi.org/10.1002/elps.8149","url":null,"abstract":"<p><p>There is abundant literature that deals with the electrophoresis of biocolloids and environmental entities. Most of these nanoparticles can be classified as soft particles, which are core-shell structured in nature. Most existing studies assume the inner core of the soft particles to be rigid in nature. However, there are various core-shell structured nanoparticles for which the inner core is semisoft in nature, which allows ion penetration but restricts fluid flow. In addition, the distribution of the peripheral layer of the soft (soft particle with rigid inner core) or semisoft (soft particle with semisoft inner core) particle is not necessarily uniform. In the present article, we consider the electrophoresis of soft and semisoft particles with the diffuse distribution of monomers across the peripheral shell layer. The mathematical model adopted here is based on the Poisson-Boltzmann equation for the electric double-layer potential and Darcy-Brinkman extended Stokes equation for fluid flow. The study is carried out considering a weak electric field assumption, which allows us to linearize the set of equations using perturbation analysis. A finite difference-based method is adopted to solve the perturbed set of equations and thus to calculate the electrophoretic mobility. The results are presented to indicate the difference in electrophoretic mobility of soft and semisoft particles under similar electrostatic conditions. We have further indicated the dependence of pertinent parameters on the electrophoretic mobility of soft or semisoft nanoparticles.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Electroosmotic Pumps to Control the Flow Velocity in Cotton Thread-Based µTADs.","authors":"Xionghui Li, Haonan Li, Xuanying Liang, Zejingqiu Chen, Muyang Zhang, Qinghao He, Jie Zhou, Zitao Feng, Yeqian Liu, Xinyi Chen, Huilin Chen, Zitong Ye, Ziwei Huang, Xingwei Zhang, Huiru Zhang, Lok Ting Chu, Weijin Guo","doi":"10.1002/elps.8153","DOIUrl":"https://doi.org/10.1002/elps.8153","url":null,"abstract":"<p><p>Flow velocity control is of great interest for passive microfluidic devices that are used in point-of-care diagnostics. Various methods have been developed for the flow velocity control of microfluidic paper-based analytical devices (µPADs), whereas fewer attempts have been made for microfluidic thread-based analytical devices (µTADs). In this research, we attempt to control the flow velocity in cotton thread-based µTADs with electroosmotic pumping. Utilizing electroosmotic pumps, the flow velocity in the cotton thread-based µTADs can be decreased or increased by 13% and 106%, respectively. Moreover, the dynamic control of the flow velocity in the cotton thread-based µTADs is achieved by adjusting the real-time magnitude and direction of the voltage. Furthermore, we demonstrate that electroosmotic pumps can be used to overcome the hydrophobic valves in the cotton thread-based µTADs. We show that the delivery sequence of different liquid samples for a three-branch µTAD can be controlled. Finally, we show the potential application in lithium detection with a colorimetric assay. This method for flow velocity control shows promise for customizing the flow velocity and reaction time of cotton thread-based µTADs, and this method can potentially increase the sensitivity of detection.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Single Nuclei in a Microfluidic Cytometer Towards Metaphase Enrichment.","authors":"Cristian Brandi, Adele De Ninno, Filippo Ruggiero, Valentina Mussi, Mauro Nanni, Federica Caselli","doi":"10.1002/elps.8152","DOIUrl":"https://doi.org/10.1002/elps.8152","url":null,"abstract":"<p><p>Identifying analyzable metaphase chromosomes is crucial for karyotyping, a common procedure used by clinicians to diagnose genetic disorders and some forms of cancer. This task is often laborious and time-consuming, making it essential to develop automated, efficient, and reliable methods to assist clinical technicians. In this work, an original label-free microfluidic approach to identify potential metaphases is developed that uses impedance-based detection of individual flowing nuclei and machine-learning-based processing of synchronized high-speed videos. Specifically, impedance signals are used to identify nucleus-containing frames, which are then processed to extract the contour of each nucleus. Feature extraction is then performed, and both unsupervised and supervised classification approaches are implemented to identify potential metaphases from those features. The proposed framework is tested on K562 cells, and the highest classification accuracy is obtained with the supervised approach coupled with a feature selection procedure and the Synthetic Minority Over-sampling Technique (SMOTE). Overall, this study encourages future developments aimed at integrating a sorting functionality in the device, thus achieving an effective microfluidic system for metaphase enrichment.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrohydrodynamic Vortex Imaging: A New Tool for Understanding Mass Transfer in Surface-Based Biosensors.","authors":"Pauline Zimmer, Oleh Andreiev, Marion Costella, Emmanuelle Laurenceau, Jean-François Bryche, Jean-Pierre Cloarec, Michael Canva, Marie Frénéa-Robin, Julien Marchalot","doi":"10.1002/elps.8137","DOIUrl":"https://doi.org/10.1002/elps.8137","url":null,"abstract":"<p><p>Surface-based biosensor performance is generally limited by mass transfer, especially when detecting low-concentrated species. To address this, dielectrophoresis (DEP) and alternating current electroosmosis (ACEO) can be combined to enhance mass transfer, increasing the target concentration near the sensor. This article presents a method for real-time direct imaging of electrohydrodynamic (EHD) effects on a microparticle suspension within a microfluidic chamber enclosed by two opposing electrodes. This top-bottom configuration was poorly studied in the literature for ACEO. The system presented thereby allows measurements of fluid flow profiles perpendicular to the electrode surface. The velocity of fluorescent latex microsphere tracers was measured as a function of signal frequency, potential, and electrolyte conductivity. This setup enables direct observation of vortices and particle-depleted areas, offering a valuable tool for selecting optimal input parameters-such as electric field, conductivity, and electrode dimensions-to efficiently concentrate microparticles near the sensor. Additionally, a numerical model developed in COMSOL and adapted for this top-bottom configuration enhances understanding of key parameters influencing EHD phenomena.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streaming-Particle Method for Dielectrophoretic Characterization.","authors":"A K M Fazlul Karim Rasel, Eron P Ristich, Mark A Hayes, Sean L Seyler","doi":"10.1002/elps.8146","DOIUrl":"https://doi.org/10.1002/elps.8146","url":null,"abstract":"<p><p>Fully characterizing subtle differences in biologically important particles-including peptides, proteins, protein complexes, exosomes, viruses, organelles, and cells-is essential, as any alteration can impact their function. Detailed bioparticle characterization has broad implications for biomedical engineering, health care, food science, astrobiology, environmental studies, and microbiology. Dielectrophoresis (DEP) generates distinct forces based on subtle structural differences between bioparticles and has the potential to enable full characterization by quantifying the DEP response of a particle. However, current DEP techniques primarily rely on particle trapping, which presents limitations, particularly for nanoparticles. In contrast, streaming-based DEP measurement techniques remain largely unexplored. Here, we introduce a streaming-based microfluidic method inspired by the (inverse) classical scattering problem in physics. Using a custom insulator-based DEP microchannel (iDEP), the DEP susceptibility of a particle is quantified based on its predictable deflection magnitude. We demonstrate the feasibility of this approach for negative DEP using finite element analysis to conduct numerical scattering experiments on representative nanoparticles. To fully capture diffusion effects, we solved the steady-state Smoluchowski advection-diffusion equation to obtain concentration fields in the microchannel and extract realistic scattering profiles. Additionally, deterministic particle trajectories, computed in the absence of diffusion, were analyzed using streamline analysis to support the advection-diffusion results. Our results indicate that, under optimal conditions, the prototype iDEP microchannel approaches the necessary sensitivity for protein DEP characterization, even when diffusion is included. Like existing iDEP devices, a real iDEP scattering instrument is expected to be easy and inexpensive to operate. Combined with the straightforward processing and interpretation of the scattering data, the iDEP scattering technique has the potential to enable high-throughput, accurate bioparticle characterization.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precise Shrinkage of Silicon Nitride Nanopores Via Externally Sourced Hydrocarbons.","authors":"Debmalya Roy, Aniruddha Guha, James Yates, Suman Chakraborty","doi":"10.1002/elps.8150","DOIUrl":"https://doi.org/10.1002/elps.8150","url":null,"abstract":"<p><p>Solid-state nanopores (SSNPs) are progressively gaining importance in biomolecular sensing and ionic circuit applications. Unlocking their full potential, however, requires the development of fabrication techniques that enable precise control over their sizes and shapes. Electron-beam (EB) shrinking provides precise, real-time feedback and is ideally suited to address these requirements. However, it necessitates an initial pore diameter smaller than the membrane thickness for effective shrinking without material addition. Typical focused ion beam (FIB)-drilled pores in silicon nitride membranes often fail to meet these requirements. Alternative efforts towards mitigating these bottlenecks through deploying hydrocarbon-mediated EB shrinkage face challenges due to uncontrolled carbon contamination or a lack thereof in cleaner transmission electron microscope (TEM) chambers. To address these challenges, here we report an alternative approach of high-precision hydrocarbon-mediated EB shrinking with hydrocarbons sourced externally through controlled surface reactions on exposure to ethanol. This provides several decisive advantages, including the reduction of pore diameters much larger than the membrane thickness and controlled shrinking in cleaner environments without contaminations. These measures accelerate nanopore fabrication, improve its predictability by eliminating the dependence on variable carbon contamination in vacuum chambers, and provide high-resolution live feedback during dimension tuning. As a result, our method supports the large-scale production of nanopores with analyte-specific, tuneable dimensions. This capability is particularly imperative for low-noise biomolecular sequencing applications that leverage electrically-modulated transport and sensing over nanoscales. These features could pave the way for the broader application of SSNPs, addressing long-standing challenges in their fabrication and functionalisation that remained unresolved thus far.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From One Strand Dyed/Undyed Hair With/Without Root to Fast and Successful STR Profiling and Evaluation With Principle Component Analysis.","authors":"Gulten Rayimoglu, Fatma Cavus Yonar, Beril Anılanmert","doi":"10.1002/elps.8142","DOIUrl":"https://doi.org/10.1002/elps.8142","url":null,"abstract":"<p><p>Fallen/shed single hair shafts with/without roots can be encountered as evidence in crime scenes. Identification from hair exhibits serious difficulties, due to the low amount of DNA, PCR inhibitors, and the existence of DNA mostly in degraded forms. Exposure of hair to chemical oxidation, sunlight, etc. brings extra difficulties to DNA analysis. In single hair shafts, no satisfactory nuDNA test method that can be used both for dyed/undyed single hair shafts with/without root exists in the literature. Next-generation systems are expensive, have a labor-intensive workflow, and are not available in many forensic laboratories. Here an STR profiling method has been developed modifying an isolation kit method for an easy, successful DNA for (1) dyed/undyed single hairs with root and (2) without root from 36 individuals and compared with the unmodified method (total 432 analyzed samples). Modified silica-based isolation method, yielded 0.65-1.05 ng/µL DNA. The success of STR typing after capillary electrophoresis was 96%-98% for dyed/undyed hair with roots and 89%-93% for rootless ones, while the results for the unmodified method were 67%-82%. The results were investigated using PCA, t-test, and F-test. This protocol is simple, inexpensive, does not require automation, and can be performed using kits/equipment available in every forensic laboratory for forensic genotyping.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the MC1R Gene Sequence Variation Using Oxford Nanopore Sequencing.","authors":"Wojciech Branicki, Aleksandra Pisarek-Pacek, Kamila Marszałek, Agata Jarosz, Magdalena Kukla-Bartoszek, Magdalena Zubańska, Agnieszka Bronikowska, Katarzyna Węgrzyn, Bożena Wysocka, Magdalena Spólnicka, Ewelina Pośpiech","doi":"10.1002/elps.8141","DOIUrl":"https://doi.org/10.1002/elps.8141","url":null,"abstract":"<p><p>The MC1R gene, which is responsible for most cases of red hair, affects other hair and skin colours and contributes to differences in pain sensitivity and consists of a single exon with a very high level of allelic heterogeneity. In this research, we show that the Oxford Nanopore Technology (ONT) offers a good alternative to study the MC1R sequence variation. MinION was used to sequence the 1590 bp MC1R exon and minimal promoter in a cohort of 126 subjects, including 65 red-haired individuals, using the FLO-MIN106 (R9.4) chemistry. Assigned DNA variants were validated using Ion Torrent technology provided with Ion Xpress Plus Fragment Library Kit and the Personal Genome Machine^TM (PGM^TM). We show that the use of the latest sequencing kit V14 together with the FLO-MIN114 (R10.4.1) flow cell has eliminated the systematic errors observed with the previous chemistry and allowed reliable detection of short indels important for phenotypic inference. Importantly, the use of the algorithm implemented in the EPI2ME software enabled convenient and accurate read-based phase determination which can be useful in data interpretation.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Size-Dependent Acoustophoresis in Rectangular Microchannels: Critical Particle Radius and Multidimensional Field Coupling Effects.","authors":"Junjun Lei","doi":"10.1002/elps.8151","DOIUrl":"https://doi.org/10.1002/elps.8151","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Particle manipulation using acoustophoresis has emerged as a pivotal technology in microsystems engineering, garnering significant attention across soft matter physics, biomedical diagnostics, and lab-on-a-chip applications. The operational efficacy of acoustofluidic tweezers platforms hinges on the precise control of the critical particle size threshold that governs the transition between radiation-force-dominated trapping and streaming-mediated transport. Through systematic numerical investigations in rectangular microchannels with two-dimensional (2D) confinement, this study establishes quantitative correlations between critical radius thresholds and geometric parameters in coupled standing-wave fields. The critical radius &lt;math&gt; &lt;semantics&gt;&lt;msubsup&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt; &lt;mi&gt;c&lt;/mi&gt;&lt;/msubsup&gt; &lt;annotation&gt;$r_p^c$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; demarcates two distinct transport regimes: (i) Supercritical particles ( &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;&gt;&lt;/mo&gt; &lt;msubsup&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt; &lt;mi&gt;c&lt;/mi&gt;&lt;/msubsup&gt; &lt;/mrow&gt; &lt;annotation&gt;${r}_p &gt; r_p^c$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) achieve stable nodal trapping via dominant radiation forces, independent of initial spatial distribution; (ii) subcritical particles ( &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;&lt;&lt;/mo&gt; &lt;msubsup&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt; &lt;mi&gt;c&lt;/mi&gt;&lt;/msubsup&gt; &lt;/mrow&gt; &lt;annotation&gt;${r}_p &lt; r_p^c$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) undergo continuous advection through streaming vortices. Our multiphysics framework combines finite element modeling with analytical validation through boundary-layer force equilibrium analysis, revealing three key findings: First, geometric confinement induces characteristic scaling laws ( &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msubsup&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt; &lt;mi&gt;c&lt;/mi&gt;&lt;/msubsup&gt; &lt;mo&gt;∝&lt;/mo&gt; &lt;msqrt&gt;&lt;mi&gt;l&lt;/mi&gt;&lt;/msqrt&gt; &lt;/mrow&gt; &lt;annotation&gt;$r_p^c propto sqrt l $&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; with &lt;math&gt;&lt;semantics&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;annotation&gt;$l$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; being the channel size) for one-dimensional (1D) standing waves, confirmed through parametric studies ( &lt;math&gt;&lt;semantics&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;annotation&gt;$l$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; : 0.1-5.1 mm). Second, 1D models maintain predictive accuracy (with discrepancies &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;γ&lt;/mi&gt; &lt;mo&gt;&lt;&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt; &lt;mo&gt;%&lt;/mo&gt;&lt;/mrow&gt; &lt;annotation&gt;$gamma &lt; 5% $&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) for systems with wavelength ratios &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mi&gt;y&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mi&gt;x&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;&gt;&lt;/mo&gt; &lt;mn&gt;4&lt;/mn&gt;&lt;/mrow&gt; &lt;annotation&gt;${lambda }_y/{lambda }_x &gt; 4$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; or &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mo&gt;&lt;&lt;/mo&gt; &lt;mn&gt;0.25&lt;/mn&gt;&lt;/mrow&gt; &lt;annotation&gt;$ &lt; 0.25$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , but fail in coupled-mode fields ( &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mn&gt;0.25&lt;/mn&gt; &lt;mspace&gt;&lt;/mspace&gt; &lt;mfrac&gt;&lt;mo&gt;&lt;&lt;/mo&gt; &lt;mo&gt;∼&lt;/mo&gt;&lt;/mfrac&gt; &lt;mspace&gt;&lt;/mspace&gt; &lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mi&gt;y&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mi&gt;x&lt;/","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Electrophoretic Mobility Determination in Nanoparticle Analysis: Two Parallel Techniques Converging in a Distinctive Parameter.","authors":"Carlos Adelantado, Jan Jordens, Stefan Voorspoels, Milica Velimirovic, Kristof Tirez","doi":"10.1002/elps.202400132","DOIUrl":"https://doi.org/10.1002/elps.202400132","url":null,"abstract":"<p><p>A critical comparison of the main parameters playing a role in measurement of electrophoretic mobility of plastic nanoparticles (NPs) by CE and laser Doppler velocimetry (LDV) techniques in NP suspensions is herein presented, accompanied by a discussion about potential impact on different mobility values observed. Capillary material and dynamic or permanent coating of the inner capillary wall, capillary dimensions, EOF variability, BGE temperature, Joule heating, and presence of species potentially interacting with analyzed NPs are underlined as possible causes of the different performance of the above two techniques. It is of importance to get an insight into the reasons behind experimental conditions and operating features to opt for one technique or the other based on research interests. In the end, it is intended to present a knowledge expansion about two parallel paths that converge in a distinctive parameter of an enormous relevance in CE, effective electrophoretic mobility, not achievable by other techniques, and discuss practical considerations in experimental design.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":"e202400132"},"PeriodicalIF":3.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}