ELECTROPHORESIS最新文献

Sialylation Impacts Separation of a Biotherapeutic by Capillary Gel Electrophoresis. 唾液化对毛细管凝胶电泳分离生物治疗药物的影响。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-07-08 DOI: 10.1002/elps.70006

Jeremy D Osko, Zhengqi Zhang, Andrew Semple, Karen Bern, Julie C McIntosh, Xiaoyu Yang, Thomas P Niedringhaus

{"title":"Sialylation Impacts Separation of a Biotherapeutic by Capillary Gel Electrophoresis.","authors":"Jeremy D Osko, Zhengqi Zhang, Andrew Semple, Karen Bern, Julie C McIntosh, Xiaoyu Yang, Thomas P Niedringhaus","doi":"10.1002/elps.70006","DOIUrl":"https://doi.org/10.1002/elps.70006","url":null,"abstract":"Monitoring of critical quality attributes (CQAs) is essential for the development of biotherapeutics. One example of a CQA is molecular fragmentation, which is often analyzed by capillary gel electrophoresis with sodium dodecyl sulfate (SDS). Sialylation is a post-translational modification and form of glycosylation that can impact purity profiles of biotherapeutics, resulting in complex structure elucidation. Here, we studied the heterogeneity of Biotherapeutic 1 as a result of O-linked glycosylation with sialylation. Biotherapeutic 1 displayed a second unidentified peak in SDS-capillary gel electrophoresis (SDS-CGE) under reducing conditions that directly impacted peak integration practices and, therefore, method validation. The two peaks were highly reproducible in SDS-CGE as well as complementary LabChip experiments. The apparent molecular weights were calculated using molecular weight ladders with known protein standards. A combination of ion exchange chromatography (IEX), hydrophilic interaction chromatography mass spectrometry (HILIC-MS), and ultra-high-performance size exclusion chromatography (UP-SEC) were used to identify O-linked glycosylation as responsible for the production of reduced peak 1 and reduced peak 2 in SDS-CGE. Specifically, reduced peak 2 contained sialylation that was not observed in reduced peak 1, resulting in two distinct migration times due to impacts in SDS binding efficacy. Enzymatic removal of the sialic acids simplified the heterogeneity into a single uniform peak (reduced peak 1). This work and methodologies highlight the impact a single O-linked glycan can have on SDS-CGE and is applicable to analyzing future biotherapeutics involving complex structure profiles resulting from sialylation.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Optimized, CE-Compatible, Targeted NGS-Based SSR Genotyping Method Using Primer-Anchored Alignment. 基于引物锚定比对的优化、ce兼容、靶向的ngs SSR基因分型方法

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-07-04 DOI: 10.1002/elps.70007

Kai Liu, Qinghui Meng, Tianlun Zheng, Nan Xie, Fan Zhou

{"title":"An Optimized, CE-Compatible, Targeted NGS-Based SSR Genotyping Method Using Primer-Anchored Alignment.","authors":"Kai Liu, Qinghui Meng, Tianlun Zheng, Nan Xie, Fan Zhou","doi":"10.1002/elps.70007","DOIUrl":"https://doi.org/10.1002/elps.70007","url":null,"abstract":"Microsatellites (SSRs) are highly polymorphic DNA sequences widely used in genetic research, including parentage assignment. Traditional SSR analysis relies on capillary electrophoresis (CE), which is time-consuming and has limited capacity. Next-generation sequencing (NGS) offers a high-throughput and cost-effective alternative, but existing NGS-based SSR genotyping methods produce results that are incompatible with CE data, increasing the risk of Mendelian inheritance mismatches. This study presents an optimized, targeted, NGS-based approach for SSR genotyping that prioritizes consistency with CE-based results. We optimized SSRseq, a targeted NGS-based SSR genotyping method, by (1) using primer flanking sequences as anchors for BLAST (Basic Local Alignment Search Tool)-based read alignment to reference SSRs, enabling the utilization of both overlapping and nonoverlapping paired-end reads; (2) inferring motif repeat counts from aligned read lengths, tolerating imperfections within the microsatellite repeat array (MRA); and (3) dynamically adjusting motif definition when discrepancies arose between expected and observed MRAs. We evaluated our optimized SSRseq against the original SSRseq and CE using four 10-plex SSR panels for parentage assignment in Largemouth black bass (Micropterus salmoides). The optimized SSRseq substantially improved parentage assignment accuracy. Multiple combinations of two or more optimized SSRseq panels achieved an assignment rate of 1.000 and an accuracy rate of 0.950, whereas the original SSRseq's highest accuracy was 0.900, requiring all four panels. The optimized method also showed high concordance with CE genotyping at several tested loci. This optimized SSRseq approach provides a robust, efficient, and cost-effective tool, leveraging NGS for accurate SSR genotyping in parentage assignment and other genetic analyses while minimizing Mendelian inheritance mismatches.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capillary Electrophoresis Can Detect the Simultaneous Presence of Hairpins and Self-Dimers in Non-Symmetric, Single-Stranded DNA Oligomers. 毛细管电泳可以检测非对称单链DNA低聚物中同时存在的发夹和自二聚体。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-07-02 DOI: 10.1002/elps.70005

Earle Stellwagen, Nancy C Stellwagen

引用次数: 0

Universal Study Design for Instrument Changes in Pharmaceutical Release Analytics. 药物释放分析中仪器变化的通用研究设计。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-06-25 DOI: 10.1002/elps.70004

Anne B Ries, Maximilian N Merkel, Kristina Coßmann, Marina Paul, Robin Grunwald, Daniel Klemmer, Franziska Hübner, Sabine Eggensperger, Frederik T Weiß

{"title":"Universal Study Design for Instrument Changes in Pharmaceutical Release Analytics.","authors":"Anne B Ries, Maximilian N Merkel, Kristina Coßmann, Marina Paul, Robin Grunwald, Daniel Klemmer, Franziska Hübner, Sabine Eggensperger, Frederik T Weiß","doi":"10.1002/elps.70004","DOIUrl":"https://doi.org/10.1002/elps.70004","url":null,"abstract":"In pharmaceutical quality control (QC), analytical methods need to maintain release analytics over decades. Over a product's lifecycle, vendors may update instrument hardware and/or software, or a switch between vendors may become necessary upon discontinuation of an instrument. Both situations pose a challenge to pharmaceutical QC.We designed an efficient instrument comparability study to gain a comprehensive understanding of potential performance differences between instruments and therefore rationalize the risk assessment and decision process for a path forward. The results may either point out whether a full or partial re-validation is necessary or whether a science-based update can be pursued based on the data generated in the study. The study design is universally applicable to a substantial range of release analytical methods. In a straightforward setup of two experiments with the new instrument, a statistically meaningful data set is generated for comparison with available historical or validation data of the original instrument. In a Good Manufacturing Practice (GMP) environment, we implemented the study design in a benchmark study comparing the ICE3 and Maurice C imaged capillary isoelectric focusing (icIEF) instruments. The core-study confirmed equal or better performance of the Maurice C in all parameters and serves as a basis for seamless continuation of release icIEF measurements on Maurice C.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peak Integration of Electropherograms in GMP and Research Labs: Navigating Increased Scrutiny Amid Data Integrity Audits and Inspections. 在GMP和研究实验室中峰值集成的电泳图：在数据完整性审计和检查中导航增加审查。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-06-19 DOI: 10.1002/elps.70002

Timothy Blanc, Hermann Wätzig, Cari Sänger-van de Griend

{"title":"Peak Integration of Electropherograms in GMP and Research Labs: Navigating Increased Scrutiny Amid Data Integrity Audits and Inspections.","authors":"Timothy Blanc, Hermann Wätzig, Cari Sänger-van de Griend","doi":"10.1002/elps.70002","DOIUrl":"10.1002/elps.70002","url":null,"abstract":"Capillary electrophoresis (CE) often offers superior separation relative to chromatography for macromolecules like monoclonal antibodies (mAbs), a major pharmaceutical class. However, electropherogram baselines pose challenges that traditional chromatography algorithms cannot address, requiring complex integration processes. Integration in good manufacturing practice (GMP) laboratories is critically important and has become a focus of data integrity-centric regulatory inspections. Electropherogram integration challenges, the increased use of CE, data systems developed for chromatograms rather than electropherograms, and the increased regulatory scrutiny call for a resolution. This necessity also extends to R&D, clinical, and academic labs. This review examines authoritative sources such as pharmacopoeias, World Health Organization (WHO), Parenteral Drug Association (PDA), and scientific literature. However, none address electropherogram integration. These sources concur that companies should develop integration policies and SOPs. Training programs must ensure analysts are proficient in integration techniques and reviewers are appropriately qualified to assess integrations. Integration parameters must be captured, including slope sensitivity, smoothing factors, and timed events like peak start and stop and baseline correction. Analytical procedures (APs) should include illustrations that define proper integration. Although automatic integration is preferred for its efficiency and objectivity, it is not always accurate. Therefore, manual integration should be permitted under specific conditions, with all settings and iterations documented, justified, and reviewed. Industry collaboration is proposed to create practical integration guidelines specifically for CE.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":"e70002"},"PeriodicalIF":3.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights Into the Enantioseparation of Polyhalogenated 4,4'-Bipyridines With a Cellulose Tris(3,5-Dimethylphenylcarbamate)-Based Chiral Column by Using Supercritical Fluid Chromatography. 超临界流体色谱法纤维素三（3,5-二甲基苯基氨基甲酸酯）手性柱分离多卤化4,4′-联吡啶对映体研究

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-05-31 DOI: 10.1002/elps.8156

Emmanuelle Lipka, Roberto Dallocchio, Barbara Sechi, Mikheil Rukhaia, Giorgi Jibuti, Bezhan Chankvetadze, Victor Mamane, Paola Peluso

{"title":"Insights Into the Enantioseparation of Polyhalogenated 4,4'-Bipyridines With a Cellulose Tris(3,5-Dimethylphenylcarbamate)-Based Chiral Column by Using Supercritical Fluid Chromatography.","authors":"Emmanuelle Lipka, Roberto Dallocchio, Barbara Sechi, Mikheil Rukhaia, Giorgi Jibuti, Bezhan Chankvetadze, Victor Mamane, Paola Peluso","doi":"10.1002/elps.8156","DOIUrl":"https://doi.org/10.1002/elps.8156","url":null,"abstract":"In the last decade, by integrating experimental and computational analyses, it was demonstrated that halogen bond (HaB) may contribute to binding and enantiorecognition mechanisms underlying the HPLC enantioseparation of halogenated chiral analytes by using cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC)-based chiral columns and n-hexane-based mixtures as mobile phases. When used as a pivotal component of the mobile phase in supercritical fluid chromatography (SFC), carbon dioxide is often considered as an n-hexane-like nonpolar solvent because of its low dielectric constant and zero molecular dipole moment. On the other hand, carbon dioxide may also serve as hydrogen bond (HB) and HaB acceptor due to the presence of nonbonding electrons on the two oxygen atoms, interacting with analyte enantiomers, chiral selectors, and co-solvents. On this basis, we report herein the results of a study aiming at evaluating the impact of using carbon dioxide in SFC in place of n-hexane in HPLC on halogen-dependent enantioseparations by using atropisomeric halogenated 4,4'-bipyridines as analytes and Lux Cellulose-1 as CDMPC-based chiral column. The experimental investigation was complemented by a computational study performed using (a) quantum mechanics (QM) calculations to map and quantify noncovalent interactions possibly underlying the contact of the analytes with carbon dioxide and with the distinctive pendant groups of the CDMPC and (b) molecular dynamics (MD) simulations to visualize noncovalent interactions acting in the analyte 1/CDMPC chromatographic system in different media. The use of MD simulations to model enantioseparations performed in carbon dioxide-based media was not reported in the literature so far.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying Critical Quality Attributes of Protein Therapeutics by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis With Native Fluorescence Detection. 十二烷基硫酸钠-毛细管凝胶电泳与天然荧光检测定量蛋白质治疗药物的关键质量属性。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-05-30 DOI: 10.1002/elps.8154

Zaifang Zhu

{"title":"Quantifying Critical Quality Attributes of Protein Therapeutics by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis With Native Fluorescence Detection.","authors":"Zaifang Zhu","doi":"10.1002/elps.8154","DOIUrl":"https://doi.org/10.1002/elps.8154","url":null,"abstract":"In the biopharmaceutical industry, the sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) assay is often used to assess therapeutic critical quality attributes (CQAs). Traditional detection methods for SDS-CGE methods, such as ultraviolet (UV) absorbance and laser-induced fluorescence (LIF), are widely used but come with limitations. A native fluorescence detection (NFD) scheme was previously developed to enhance sensitivity and reduce gel matrix interference without requiring sample derivatization, and the SDS-CGE-NFD assay exhibited high precision and accuracy for absolute quantification of monoclonal antibodies (mAbs). In this work, we assessed the suitability of SDS-CGE-NFD to quantification of CQAs in protein therapeutics, which is generally relative rather than absolute. NFD was compared with UV absorbance and LIF detection for quantifying CQAs of protein therapeutics in SDS-CGE. Three lots of NIST monoclonal antibody (NISTmAb) were assayed by SDS-CGE with NFD, UV, and LIF detection, and the relative abundance of total fragments was compared and found similar. Analysis with NFD measured abundances at a range of 1.77%-2.00%, compared to the range of 1.53%-1.78% measured with UV absorbance and 1.63%-1.86% measured with LIF. Aggregates were not recognized with UV absorbance but were apparent with measured relative abundance of 0.38%-0.40% using NFD and 0.35%-0.40% using LIF. Under the reducing conditions, glycosylation site occupancy on the heavy chain was measured in the range of 99.30%-99.33% with all three detection approaches. The comparable results measured with three detection modes suggested that SDS-CGE-NFD was suitable to quantify CQAs of protein therapeutics. The SDS-CGE-NFD workflow was successfully applied to analyze two commercial protein therapeutics, a bispecific mAb of ∼146 kDa (Emicizumab) and an Fc-fusion protein of ∼63 kDa (Dulaglutide).","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Separation and Analysis of Exosomes in Milk Sample by on-Line Nano-Liquid Chromatography. 在线纳米液相色谱法快速分离和分析牛奶样品中的外泌体。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-05-21 DOI: 10.1002/elps.8155

Cemil Aydoğan, Zeynep Günyel, Sarah Alharthi, Hakiye Aslan, İbrahim Y Erdoğan, Ziad El Rassi

{"title":"Rapid Separation and Analysis of Exosomes in Milk Sample by on-Line Nano-Liquid Chromatography.","authors":"Cemil Aydoğan, Zeynep Günyel, Sarah Alharthi, Hakiye Aslan, İbrahim Y Erdoğan, Ziad El Rassi","doi":"10.1002/elps.8155","DOIUrl":"https://doi.org/10.1002/elps.8155","url":null,"abstract":"Exosomes are very small vesicles of 30-150 nm average particle size and hold great potential in new therapeutic applications. The aim of this study is to develop a new method for the isolation and analysis of exosomes in milk via hydrophobic interaction chromatography (HIC), including salting-out process in nano-liquid chromatography (nano-LC). On the basis of this approach, a trap column combined with graphene oxide (GO)-based monolithic nano-column was used for on-line analysis of exosomes in nano-LC. The monolith was prepared by an in situ polymerization of butyl methacrylate (BMA), ethylene glycol dimethacrylate (EDMA), and methacryloyl graphene oxide nanoparticles (MGONPs). The final solution was introduced into a fused silica capillary with a 50 µm i.d. for polymerization. After preparation, the column was further modified with dimethyloctadecylchlorosilane (DODCS) to increase its hydrophobicity. The characterization of monolith was performed using scanning electron microscopy (SEM) and chromatographic examination. The final monolith was applied for the isolation and analysis of exosomes in milk via HIC-nano-LC. Nanoparticle tracking analysis (NTA), SEM, and Fourier transform-infrared (FT-IR) for the tandem characterization of milk exosomes were used, whereas step gradient elution was employed for HIC. The results demonstrated good ability to isolate exosomes from milk with three dilution factors, and a loading capacity of 7.3 ± 02 × 1011 exosomes could be obtained using the on-line nano-LC system. The developed method holds many advantages and may be adapted for the isolation of exosomes from a diverse range of media.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Study on the Electrophoresis of Soft and Semisoft Nanoparticles. 软质和半软质纳米颗粒电泳的比较研究。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-05-10 DOI: 10.1002/elps.8149

Saurabh K Maurya, Santanu Saha, Partha P Gopmandal

{"title":"Comparative Study on the Electrophoresis of Soft and Semisoft Nanoparticles.","authors":"Saurabh K Maurya, Santanu Saha, Partha P Gopmandal","doi":"10.1002/elps.8149","DOIUrl":"https://doi.org/10.1002/elps.8149","url":null,"abstract":"There is abundant literature that deals with the electrophoresis of biocolloids and environmental entities. Most of these nanoparticles can be classified as soft particles, which are core-shell structured in nature. Most existing studies assume the inner core of the soft particles to be rigid in nature. However, there are various core-shell structured nanoparticles for which the inner core is semisoft in nature, which allows ion penetration but restricts fluid flow. In addition, the distribution of the peripheral layer of the soft (soft particle with rigid inner core) or semisoft (soft particle with semisoft inner core) particle is not necessarily uniform. In the present article, we consider the electrophoresis of soft and semisoft particles with the diffuse distribution of monomers across the peripheral shell layer. The mathematical model adopted here is based on the Poisson-Boltzmann equation for the electric double-layer potential and Darcy-Brinkman extended Stokes equation for fluid flow. The study is carried out considering a weak electric field assumption, which allows us to linearize the set of equations using perturbation analysis. A finite difference-based method is adopted to solve the perturbed set of equations and thus to calculate the electrophoretic mobility. The results are presented to indicate the difference in electrophoretic mobility of soft and semisoft particles under similar electrostatic conditions. We have further indicated the dependence of pertinent parameters on the electrophoretic mobility of soft or semisoft nanoparticles.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Electroosmotic Pumps to Control the Flow Velocity in Cotton Thread-Based µTADs. 利用电渗透泵控制棉线微tad的流速。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-05-10 DOI: 10.1002/elps.8153

Xionghui Li, Haonan Li, Xuanying Liang, Zejingqiu Chen, Muyang Zhang, Qinghao He, Jie Zhou, Zitao Feng, Yeqian Liu, Xinyi Chen, Huilin Chen, Zitong Ye, Ziwei Huang, Xingwei Zhang, Huiru Zhang, Lok Ting Chu, Weijin Guo

{"title":"Using Electroosmotic Pumps to Control the Flow Velocity in Cotton Thread-Based µTADs.","authors":"Xionghui Li, Haonan Li, Xuanying Liang, Zejingqiu Chen, Muyang Zhang, Qinghao He, Jie Zhou, Zitao Feng, Yeqian Liu, Xinyi Chen, Huilin Chen, Zitong Ye, Ziwei Huang, Xingwei Zhang, Huiru Zhang, Lok Ting Chu, Weijin Guo","doi":"10.1002/elps.8153","DOIUrl":"https://doi.org/10.1002/elps.8153","url":null,"abstract":"Flow velocity control is of great interest for passive microfluidic devices that are used in point-of-care diagnostics. Various methods have been developed for the flow velocity control of microfluidic paper-based analytical devices (µPADs), whereas fewer attempts have been made for microfluidic thread-based analytical devices (µTADs). In this research, we attempt to control the flow velocity in cotton thread-based µTADs with electroosmotic pumping. Utilizing electroosmotic pumps, the flow velocity in the cotton thread-based µTADs can be decreased or increased by 13% and 106%, respectively. Moreover, the dynamic control of the flow velocity in the cotton thread-based µTADs is achieved by adjusting the real-time magnitude and direction of the voltage. Furthermore, we demonstrate that electroosmotic pumps can be used to overcome the hydrophobic valves in the cotton thread-based µTADs. We show that the delivery sequence of different liquid samples for a three-branch µTAD can be controlled. Finally, we show the potential application in lithium detection with a colorimetric assay. This method for flow velocity control shows promise for customizing the flow velocity and reaction time of cotton thread-based µTADs, and this method can potentially increase the sensitivity of detection.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0