ELECTROPHORESIS最新文献_第2页

Novel Applications of CE-ICP-MS/MS: Monitoring of Antiaging GHK-Cu Cosmetic Component Encapsulation in Liposomes. CE-ICP-MS/MS 的新应用：监测脂质体中封装的抗衰老 GHK-Cu 化妆品成分。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-25 DOI: 10.1002/elps.202400047

Joanna Zajda, Emilia Wadych, Karolina Ogórek, Marcin Drozd, Magdalena Matczuk

{"title":"Novel Applications of CE-ICP-MS/MS: Monitoring of Antiaging GHK-Cu Cosmetic Component Encapsulation in Liposomes.","authors":"Joanna Zajda, Emilia Wadych, Karolina Ogórek, Marcin Drozd, Magdalena Matczuk","doi":"10.1002/elps.202400047","DOIUrl":"https://doi.org/10.1002/elps.202400047","url":null,"abstract":"The hyphenation of the separation technique with the high-sensitive mass spectrometry detection is one of the driving forces of modern analysis enabling measurements in complex matrices. In particular, capillary electrophoresis coupled to inductively coupled plasma tandem mass spectrometry allows for speciation analysis of selected analytes with a superior resolution. The mild, physiological-friendly conditions of this separation technique offer the unique advantage of analyzing chemical entities in their intact form, which has been successfully exploited in various areas. Herein, we report the pioneering application of such a hyphenated technique in the cosmetic field to investigate the encapsulation of copper tripeptide complex (GHK-Cu) in liposomes. By monitoring copper and phosphorus signals, the formation of liposomes via a simple ethanol injection method was confirmed, and the concentration of GHK-Cu in the liposomes was assessed. The application of coupling of capillary electrophoresis with inductively coupled plasma tandem mass spectrometry (CE-ICP-MS/MS) in cosmetic studies could lead to the development of diverse liposomal formulations with preferential properties and expand their accessibility.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Method Validation for the Determination of Ciprofloxacin in Lucilia sericata Larvae via Capillary Electrophoresis Combined With Mass Spectrometry. 通过毛细管电泳结合质谱法测定蚕蛾幼虫体内环丙沙星的方法验证

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-25 DOI: 10.1002/elps.202400118

Magdalena Czuma-Pokusa, Maria Walczak

{"title":"Method Validation for the Determination of Ciprofloxacin in Lucilia sericata Larvae via Capillary Electrophoresis Combined With Mass Spectrometry.","authors":"Magdalena Czuma-Pokusa, Maria Walczak","doi":"10.1002/elps.202400118","DOIUrl":"https://doi.org/10.1002/elps.202400118","url":null,"abstract":"Substances derived from insects can serve therapeutic functions due to their diverse biological properties. This article focuses on the species Lucilia sericata and the benefits of larval therapy in patients who, due to hospitalization, have developed pressure ulcers and other difficult-to-heal wounds. Larval therapy, also known as maggot debridement therapy, employs sterile fly larvae to treat chronic, non-healing wounds by enzymatically degrading necrotic tissue and decreasing bacterial colonization. The larvae are applied to the wound for a period of 48-72 h, during which they effectively clean the wound and stimulate tissue regeneration. This therapeutic approach is particularly efficacious for recalcitrant wounds, such as diabetic foot ulcers and pressure sores, which have not responded to conventional treatments. Larvae may also constitute an alternative material in entomotoxicological studies to detect substances ingested at not only toxic but also therapeutic doses. The present work describes a method for assaying ciprofloxacin in L. sericata larvae using capillary electrophoresis coupled to mass spectrometry. The developed method features high sensitivity with a limit of quantification of 100 ± 0.018 ng/mL, as well as accuracy and precision estimated within 87%-103% and 1%-4%, respectively. An application of a simple and fast precipitation of proteins procedure for sample cleaning resulted in a highly satisfactory recovery of the analyte (90%-104%). The method was linear in a range of 100-1000 ng/mL with a determination coefficient higher than 0.9973. The method was used to determine ciprofloxacin in larval homogenate after antibiotic administration to the patient at a dose of 500 mg twice daily per os during application of the larvae dressing. Ciprofloxacin was shown to distribute from the patient's circulation to the larvae at a concentration of 150 ng/mL (750 ng/g).","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board: Electrophoresis 19–20'24 编辑委员会：电泳 19-20'24

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-24 DOI: 10.1002/elps.202470102

引用次数: 0

Contents: Electrophoresis 19–20'24 内容：电泳 19-20'24

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-24 DOI: 10.1002/elps.202470103

引用次数: 0

RETRACTION: A convenient guideline to determine if two Y-STR profiles are from the same lineage. 回归：确定两个 Y-STR 图谱是否来自同一血统的便捷准则。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-14 DOI: 10.1002/elps.8055

引用次数: 0

Applications of Probabilistic Genotyping Method for Combining Evidence Across Microhaplotype DNA Mixture Profiles. 应用概率基因分型方法综合微单型 DNA 混合图谱中的证据

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-14 DOI: 10.1002/elps.202400140

Xiaohua Ling, Shuang Han, Xinyi Lin, Zhaochen Bai, Nan Zhang, Jiayue Li, Huan Wang, Xueling Ou

{"title":"Applications of Probabilistic Genotyping Method for Combining Evidence Across Microhaplotype DNA Mixture Profiles.","authors":"Xiaohua Ling, Shuang Han, Xinyi Lin, Zhaochen Bai, Nan Zhang, Jiayue Li, Huan Wang, Xueling Ou","doi":"10.1002/elps.202400140","DOIUrl":"https://doi.org/10.1002/elps.202400140","url":null,"abstract":"In cases of serious crimes that involve challenging DNA samples from the perpetrator (e.g., a minor contributor to a mixture), there is justification to combine different mixture profiles. In our previous study, we developed a massively parallel sequencing (MPS)-based assay targeting 140 microhaplotype markers. In this study, we extended the use of the microhaplotype panel to common scenarios, such as determining the presence of a common contributor or relatedness between different mixture profiles when no reference source is available. Data interpretation was performed using the R package KinMix. Our findings revealed that correct assignments of a common contributor and relatedness were made between relatively balanced mixtures. However, when profiles suffered from allele imbalance, inclusive assignments were significantly associated with the suspect's mixture proportion. Additionally, our analysis showed zero false-positive rates in the studied scenarios. These results indicate that microhaplotype data can be reliably interpreted for identifying a common donor or related donors among different mixtures. Further research based on larger sample sizes may yield more reliable results, which could assist in solving issues related to complex scenarios where multiple mixed profiles were involved.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forensic STR and SNP Genotyping of Formalin-Fixed Skeleton Samples With Illumina's ForenSeq System. 利用 Illumina 的 ForenSeq 系统对福尔马林固定骸骨样本进行法医 STR 和 SNP 基因分型。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-14 DOI: 10.1002/elps.202400056

Tingting Yang, Linlin Gao, Jiarong Zhang, Wei Xie, Wenjing Hu, Jiangwei Yan

{"title":"Forensic STR and SNP Genotyping of Formalin-Fixed Skeleton Samples With Illumina's ForenSeq System.","authors":"Tingting Yang, Linlin Gao, Jiarong Zhang, Wei Xie, Wenjing Hu, Jiangwei Yan","doi":"10.1002/elps.202400056","DOIUrl":"https://doi.org/10.1002/elps.202400056","url":null,"abstract":"Formalin fixatives are widely used in forensics to preserve tissues. However, extracting high-quality genomic DNA from formalin-fixed samples is challenging. Traditional short tandem repeat (STR) analysis using capillary electrophoresis (CE) for forensic DNA typing frequently results in failure. Massively parallel sequencing (MPS) can handle many samples and thousands of genetic markers, usually single-nucleotide polymorphisms (SNPs) and STRs, in a single test. Thus, it is useful for assessing highly deteriorated forensic evidence. Few studies have examined the effectiveness of STRs and SNPs genotyping of formalin-fixed skeletons using MPS. In this study, 55 skeletal samples from 5 individuals that were treated under different formalin fixation times (5-75 days) were examined and sequenced using the ForenSeq DNA Signature Prep Kit on the Illumina MiSeq FGX platform. The results showed that as the duration of formalin fixation increased, the detection rates of STRs and SNPs gradually decreased. After 75 days of fixation, the average detection rates for STRs and SNPs were 4% and 10%, respectively. The cumulative discrimination power (CDP) of individual identification SNPs (iiSNPs) was >0.9999 on the 45th day. However, the CDP of STRs was 0.9930 on the 22nd day. Low detection rates were observed for six STRs (D1S1656, PentaE, D22S1045, PentaD, DX8378 and DX10103) and five SNPs (rs2920816, rs354439, rs1736442, rs338882 and rs1031825). In conclusion, DNA extracted from formalin-fixed skeletons decomposes rapidly over time, and MPS technology can be a useful tool for detecting forensic genetic markers in such samples.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic Age Estimation by Detecting DNA Methylation Status in Buccal Swabs. 通过检测口腔拭子中的 DNA 甲基化状态估算表观遗传学年龄

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-14 DOI: 10.1002/elps.202400075

Zimeng Guan, Jiaqi Wang, Zidong Liu, Chengwen Yang, Xin Xu, Xinjie Wang, Gengqian Zhang

{"title":"Epigenetic Age Estimation by Detecting DNA Methylation Status in Buccal Swabs.","authors":"Zimeng Guan, Jiaqi Wang, Zidong Liu, Chengwen Yang, Xin Xu, Xinjie Wang, Gengqian Zhang","doi":"10.1002/elps.202400075","DOIUrl":"https://doi.org/10.1002/elps.202400075","url":null,"abstract":"The analysis of DNA methylation (DNAm) levels at specific CpG sites represents one of the most promising molecular techniques for estimating an individual's age. To date, a considerable number of studies have reported the development of age prediction models on the basis of DNAm in body fluids, with only a few utilizing buccal swabs. The objective of this study was to identify age-dependent methylation CpG sites in three different genes (HOXC4, TRIM59, and ELOVL2) in buccal swab samples from the Chinese Han population. A total of 461 buccal swabs, with an age range of 0.4-80.8 years, were divided into a training set (n = 325) and a validation set (n = 136). Samples were analyzed by pyrosequencing in order to identify age-related genes with correlation coefficient. A random forest regression model was ultimately proposed, including eight CpGs in three genes, with a mean absolute error (MAE) of 2.119 years. The model performs independent validation set with an MAE of 4.391 years. Our findings illustrate that buccal swabs present a suitable alternative to biological traces for age prediction based on DNAm pattern using pyrosequencing and random forest regression, offering the additional advantage of being collected noninvasively.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Genotyping of Three SNVs, rs5471, rs5472, and rs2000999 Involved in Serum Haptoglobin Levels by Fluorescent Probe-Based Melting Curve Analysis. 通过基于荧光探针的熔融曲线分析法同时对涉及血清aptoglobin水平的三个SNV（rs5471、rs5472和rs2000999）进行基因分型

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-14 DOI: 10.1002/elps.202400172

Mikiko Soejima, Yoshiro Koda

{"title":"Simultaneous Genotyping of Three SNVs, rs5471, rs5472, and rs2000999 Involved in Serum Haptoglobin Levels by Fluorescent Probe-Based Melting Curve Analysis.","authors":"Mikiko Soejima, Yoshiro Koda","doi":"10.1002/elps.202400172","DOIUrl":"https://doi.org/10.1002/elps.202400172","url":null,"abstract":"Haptoglobin (Hp) is a hemoglobin-binding acute-phase serum protein. Several single nucleotide variations (SNVs) within the Hp gene (HP) or Hp-related protein gene (HPR), such as rs5471 (A > C) and rs5472 (A > G) in HP promoter region and rs2000999 (G > A) in intron 2 of HRP, are suggested to correlate with the serum Hp levels. To determine these three SNVs simultaneously, a genotyping assay based on duplex dual-labeled fluorescent probes was developed. The method was then validated by analyzing genomic DNA from 121 Ghanaian and two Japanese subjects who had been previously genotyped for rs5471, rs5472, and rs2000999. Both rs5471 and rs5472 could be determined as haplotypes with a single FAM-labeled fluorescent probe, and rs2000999 could be genotyped with a HEX-labeled fluorescent probe. The results obtained with the present method were consistent with the previous results except for those of three Ghanaian subjects. All three subjects appear to have multiple HPR copy number variants characteristic of African populations, which may have led to incorrect results during previous genotyping. This method allows us to genotype these three SNVs in a relatively large number of samples, especially in African populations where rs5471 is uniquely distributed.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suspended Stir Bar Sorptive Extraction Based on Zr-MOF-Modified Cotton Thread for Enrichment and Detection of Three Trace Quinolones in Fish Tissue. 基于 Zr-MOF 改性棉线的悬浮搅拌棒吸附萃取法富集和检测鱼组织中的三种痕量喹诺酮类药物

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-10-14 DOI: 10.1002/elps.202400096

Jiao Long, Yan Gao, Kangjia Sheng, Tao Bao, Sicen Wang

引用次数: 0