ELECTROPHORESIS最新文献_第2页

Recent Advances on Liquid-Phase Separation Techniques in Liquid Biopsy Technology for Detecting Circulating Cancer Biomarkers 液体活检技术中液相分离技术检测循环肿瘤生物标志物的研究进展。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-10-16 Epub Date: 2026-07-19 DOI: 10.1002/elps.70134

Elif Burcu Aydın, Muhammet Aydın, Mustafa Kemal Sezgintürk

{"title":"Recent Advances on Liquid-Phase Separation Techniques in Liquid Biopsy Technology for Detecting Circulating Cancer Biomarkers","authors":"Elif Burcu Aydın, Muhammet Aydın, Mustafa Kemal Sezgintürk","doi":"10.1002/elps.70134","DOIUrl":"10.1002/elps.70134","url":null,"abstract":"<div>\u0000 \u0000 Globally, cancer morbidity and death are rising quickly, making it imperative to develop a workable plan for early cancer patient identification and treatment outcome prediction. Liquid biopsy, a minimally invasive and repeatable technique, can replace the restrictions of tissue biopsy by detecting, assessing, and tracking cancer in any bodily fluid, including human blood. This novel diagnostic technique offers crucial data for tracking and recognizing tumor genomes in samples of physiological fluids. Through minimally invasive methods, the identification of tumor-origin biological components, such as circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), circulating microRNAs (miRNAs), exosomes, and autoantibodies in serum, plasma, saliva, and urine, allows for disease monitoring, early diagnosis, and assessment of treatment response. The goal of this study is to provide a comprehensive summary of the most recent discoveries and applications of liquid biopsy technology for detecting cancer biomarkers, with an emphasis on the years 2005–2025. When used in conjunction with other test results, liquid biopsy is also advantageous for early recurrence detection, monitoring patients who are considered to be at high risk of getting cancer and improving diagnosis. Combining patient history, clinical results, and biopsy biomarker amounts will enable more precise and timely diagnosis thanks to developments in analytical methods and informatics. This review thoroughly examined the biosynthesis, significance, and potential role of four well-known biological markers in tumor diagnosis and treatment. In addition, the advances and challenges in detection technologies for these analytes, as well as potential solutions to address these issues, were also discussed. Finally, we discuss the advantages and disadvantages of liquid biopsy, along with suggestions for its future advancement.\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 10","pages":"964-981"},"PeriodicalIF":2.6,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Micellar Electrokinetic Chromatography Screening Tool for Antiepileptic Drugs 一种抗癫痫药物胶束电动色谱筛选工具。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-10-16 Epub Date: 2026-08-07 DOI: 10.1002/elps.70137

Hailekiros Gebretsadik Kidanemariam, Erwin Adams, Ann Van Schepdael

{"title":"A Micellar Electrokinetic Chromatography Screening Tool for Antiepileptic Drugs","authors":"Hailekiros Gebretsadik Kidanemariam, Erwin Adams, Ann Van Schepdael","doi":"10.1002/elps.70137","DOIUrl":"10.1002/elps.70137","url":null,"abstract":"<div>\u0000 \u0000 This study presents an affordable micellar electrokinetic chromatography (MEKC) method for the simultaneous analysis of six commonly used antiepileptic drugs: ethosuximide, phenobarbital, lamotrigine, phenytoin, carbamazepine, and diazepam. Systematic optimization of the method resulted in a running buffer containing 80 mM sodium dodecyl sulfate, 5% methanol, 2.5 mM phosphate, and 10 mM borate at pH 8.7, which provided robust and reliable separation. The method demonstrated excellent precision, with migration time %RSD values below 2% for both intraday and inter-day repeatability, and peak area %RSD values within acceptable limits (1.7%–3.7% intraday; 2.9%–6.3% inter-day). Linearity was confirmed for all analytes, with r2 values exceeding 0.99 and residuals randomly distributed around zero. Robustness testing showed that small variations in electrophoretic conditions did not significantly affect the resolution of the critical analyte pairs, which consistently remained above 1.5. Application of the method to five antiepileptic tablet formulations obtained from the Ethiopian market demonstrated its suitability as a rapid, reliable, and cost-effective screening tool. Overall, the developed MEKC method provides a practical analytical approach for antiepileptic medicines’ quality assessment in resource-limited settings, supporting efforts to strengthen regulatory surveillance and safeguard public health.\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 10","pages":"939-949"},"PeriodicalIF":2.6,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Derived Moving Reaction Boundary Initially Created by Formic Buffer and Sodium Hydroxide in Capillary Electrophoresis: Theoretic and Experimental Study. 甲酸缓冲液和氢氧化钠在毛细管电泳中初步形成的移动反应边界：理论和实验研究。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-10-15 DOI: 10.1002/elps.70168

Jing Gao, Weiwen Liu, Qiang Zhang, Chengxi Cao

引用次数: 0

Preparation of Monodispersed Molecularly Imprinted Polymers for Abiraterone and Its Derivatives and Evaluation of Their Retention and Molecular Recognition Properties. 阿比特龙及其衍生物单分散分子印迹聚合物的制备及其保留和分子识别性能的评价。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-10-13 DOI: 10.1002/elps.70169

Hiromi Kanji, Chie Honda, Noboru Hayama, Jun Haginaka

{"title":"Preparation of Monodispersed Molecularly Imprinted Polymers for Abiraterone and Its Derivatives and Evaluation of Their Retention and Molecular Recognition Properties.","authors":"Hiromi Kanji, Chie Honda, Noboru Hayama, Jun Haginaka","doi":"10.1002/elps.70169","DOIUrl":"https://doi.org/10.1002/elps.70169","url":null,"abstract":"Monodispersed molecularly imprinted polymers (MIPs) for abiraterone (Abi), 5'-chloroabiraterone (5'-ClAbi), 5'-methylabiraterone (5'-MeAbi), and 6'-methylabiraterone (6'-MeAbi) (MIPAbi, MIP5'-ClAbi, MIP5'-MeAbi, and MIP6'-MeAbi, respectively) were prepared using methacrylic acid (MAA) and ethylene glycol dimethacrylate (EDMA) as a functional monomer and a crosslinker, respectively, by multistep swelling and polymerization. The molar ratio of the template molecule to MAA and EDMA was 2:7:25. The retention and molecular recognition properties of the non-imprinted polymer and MIPs (MIPAbi, MIP5'-ClAbi, MIP5'-MeAbi, and MIP6'-MeAbi) were evaluated using a mobile phase consisting of sodium phosphate buffer and acetonitrile in reversed-phase liquid chromatography (LC). Abi, 5'-MeAbi, and 6'-MeAbi showed the highest retention on these MIPs in a mobile phase consisting of sodium phosphate buffer and acetonitrile (50:50) at pH 5.7, whereas 5'-ClAbi exhibited the highest retention in a mobile phase consisting of sodium phosphate buffer and acetonitrile (50:50) at pH 3.7. On the other hand, the imprinting factors of the template molecules followed the order MIP5'-ClAbi < MIP5'-MeAbi < MIP6'-MeAbi < MIPAbi. The DFT calculations suggested two hydrogen‑bonding interactions between the (substituted) pyridines and the hydroxy groups of Abi and its derivatives with MAAs in the gas phase. The interaction energies followed the order MIP5'-ClAbi < MIPAbi < MIP5'-MeAbi < MIP6'-MeAbi. These results suggested that ionic or hydrogen-bonding interactions and hydrophobic interactions could work for the retention and molecular recognition of Abi and its derivatives on these MIPs in reversed-phase mode.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149179940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the Operating Conditions for a Referenced Contactless Conductivity Detector for Capillary Zone Electrophoresis. 毛细管区带电泳参考非接触式电导率检测器工作条件的研究。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-10-12 DOI: 10.1002/elps.70167

Giacomo Musile, Marc-Aurèle Boillat, Peter C Hauser

引用次数: 0

A Highly Efficient DLD Microfluidic Chip With Two-Stage Inclined Elliptical Pillars for Particle Separation and Purification. 一种用于颗粒分离纯化的两级倾斜椭圆柱高效DLD微流控芯片。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-10-09 DOI: 10.1002/elps.70164

Di Jiang, Yujiang Li, Jiawen Guo, Pengcheng Zhao, Bin Wu

{"title":"A Highly Efficient DLD Microfluidic Chip With Two-Stage Inclined Elliptical Pillars for Particle Separation and Purification.","authors":"Di Jiang, Yujiang Li, Jiawen Guo, Pengcheng Zhao, Bin Wu","doi":"10.1002/elps.70164","DOIUrl":"https://doi.org/10.1002/elps.70164","url":null,"abstract":"Deterministic lateral displacement (DLD), a hydrodynamic particle manipulation technology, has been extensively studied for the development of fast and efficient microfluidic chips for particle manipulation and detection. However, particles are prone to clogging in DLD chips with closely spaced pillars, which can reduce sorting efficiency. Previous studies have shown that elliptical pillars provide better separation performance than equilateral triangular pillars. In elliptical-pillar DLD, a negative angle of inclination (Aoi) reduces the particle period and facilitates efficient isolation of large particles, whereas a positive Aoi decreases the critical diameter, thereby improving the sorting of smaller particles. On the basis of these findings, we designed a two-stage DLD microfluidic chip with inclined elliptical-pillar arrays, using an Aoi of -45° in the first stage and 45° in the second stage. The combination of negative and positive Aoi values enhanced the isolation of large particles, improved the purification of medium- and small-sized particles, and reduced the risk of clogging. This design provides a practical approach for improving the separation and purification performance of DLD devices and offers potential for efficient multi-size particle separation.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149148311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Legionella Bacteria Using a Silicon-Based Photonic Microring Resonator Biosensor Enhanced by Dielectrophoresis. 介质电泳增强硅基光子微环谐振生物传感器检测军团菌。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-10-09 DOI: 10.1002/elps.70165

Anders Henriksson, Philipp Schrenk, Jamie R K Marland, Andreas Tsiamis, Ha Duong Ngo, Jörg König, David Schreier, Stewart Smith, Han Zhang, Arum Han, Peter Neubauer, Mario Birkholz

引用次数: 0

Chiral Ionic Covalent Organic Framework Core-Shell Composite Material CD-COF-Li@SiO₂ for HPLC Enantioseparation. 手性离子共价有机骨架核壳复合材料CD-COF-Li@SiO2用于HPLC对映体分离。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-10-06 DOI: 10.1002/elps.70162

Chang-Xiu Ma, Hong-Mei Zhou, Fu Li, Jin Liu, Xiao-Fang Shu, Peng-Bo Du, Sheng-Ming Xie, Kun-Ming Jiang, Bang-Jin Wang, Jun-Hui Zhang, Li-Ming Yuan

{"title":"Chiral Ionic Covalent Organic Framework Core-Shell Composite Material CD-COF-Li@SiO2 for HPLC Enantioseparation.","authors":"Chang-Xiu Ma, Hong-Mei Zhou, Fu Li, Jin Liu, Xiao-Fang Shu, Peng-Bo Du, Sheng-Ming Xie, Kun-Ming Jiang, Bang-Jin Wang, Jun-Hui Zhang, Li-Ming Yuan","doi":"10.1002/elps.70162","DOIUrl":"https://doi.org/10.1002/elps.70162","url":null,"abstract":"Ionic covalent organic frameworks (iCOFs), as a branch of emerging organic framework materials, integrate charged functional sites with periodically ordered pore structures. Owing to their distinctive merits, including adjustable electrostatic interactions, permanent porosity, well-defined pore channels, and excellent structural designability, iCOFs have aroused widespread research interest. In this study, a novel HPLC chiral stationary phase (CD-COF-Li@SiO2) was prepared by an in situ growth strategy. The CD-COF-Li@SiO2 column showed good chiral separation performance for 19 pairs of enantiomers, including alcohols, esters, ketones, organic acids, and aldehydes. Most of the analytes were baseline-separated, and the resolution of some enantiomers (1-(4-chlorophenyl) ethanol and 2-chloro-2-phenylacetophenone) reached 3.00. Compared with CTpBD@SiO2, β-CD-COF@SiO2, and two widely used commercial chiral columns (Chiralpak AD-H and Chiralcel OD-H), it was found that the fabricated column had good chiral recognition complementarity with four chiral chromatographic columns. Furthermore, the effects of the injection mass of the analyte, column temperature, and the proportion of the mobile phase on the separation of racemates were also discussed. This column exhibited good reproducibility and stability. After hundreds of injections, the relative standard deviation (RSD) values of retention time and resolution were both below 1%. In addition, the proposed CD-COF-Li@SiO2-based HPLC method demonstrated favorable performance for quantitative analysis by using (S)-ethyl mandelate as a model analyte. Accordingly, the limit of detection value of 0.38 µg·mL-1 and the limit of quantitation value of 1.26 µg·mL-1 for (S)-ethyl mandelate detection were obtained. This work highlights the promising prospects of chiral iCOFs for enantioseparation in HPLC.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophoretic Approaches for Investigating Plant-Derived Compound Interactions With DNA and Proteins. 研究植物源化合物与DNA和蛋白质相互作用的电泳方法。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-09-29 DOI: 10.1002/elps.70163

Zeynep Aydemir, Beril S Kaya, Meysera Bakırcı, Abdullah Al Faysal, Zeynep Kalaycıoğlu, Ayşegül Gölcü

{"title":"Electrophoretic Approaches for Investigating Plant-Derived Compound Interactions With DNA and Proteins.","authors":"Zeynep Aydemir, Beril S Kaya, Meysera Bakırcı, Abdullah Al Faysal, Zeynep Kalaycıoğlu, Ayşegül Gölcü","doi":"10.1002/elps.70163","DOIUrl":"https://doi.org/10.1002/elps.70163","url":null,"abstract":"Plant-derived compounds are increasingly recognized as promising therapeutic agents due to their broad biological activities and structural diversity, making their interactions with DNA and proteins central to understanding mechanisms of action and guiding drug discovery. Electrophoretic techniques probe these interactions by tracking changes in electrophoretic mobility, conformation, complex formation, and biopolymer integrity. This review surveys electrophoretic strategies for characterizing plant-derived compound-DNA and -protein interactions, covering fundamental DNA-binding modes (intercalation, groove binding, electrostatic association, cleavage), representative phytochemicals and extracts, and analytical challenges of complex plant matrices, including chemical heterogeneity, synergistic and antagonistic effects, and active-constituent identification. Particular attention is given to capillary electrophoresis-based approaches (affinity CE, CE, and pressure-assisted frontal analysis) and gel-based methods (agarose gel electrophoresis, native PAGE, SDS-PAGE). The literature shows capillary electrophoresis excels at quantifying binding affinity, stoichiometry, and specificity, whereas gel electrophoresis better visualizes ligand-induced changes in DNA topology and protein behavior. HSA and BSA dominate as model proteins, while advanced methods remain underapplied to chemically complex extracts, gaps pointing toward broader protein targets and natural-product systems. Integrating mass spectrometry, advanced detection, and data-driven approaches could further strengthen the mechanistic and quantitative reach of electrophoretic analysis in this field.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149080200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved Peptide Recovery in Low-Input MS-Based Proteomics Using High-Recovery StageTip Desalting and Nonionic Detergent-Assisted Reconstitution. 利用高回收率阶段脱盐和非离子型洗涤剂辅助重构提高低输入MS-Based蛋白质组学的肽回收率。

IF 2.6 3区生物学

ELECTROPHORESIS Pub Date : 2026-09-23 DOI: 10.1002/elps.70161

Akane Omori, Eisuke Kanao, Yasushi Ishihama

{"title":"Improved Peptide Recovery in Low-Input MS-Based Proteomics Using High-Recovery StageTip Desalting and Nonionic Detergent-Assisted Reconstitution.","authors":"Akane Omori, Eisuke Kanao, Yasushi Ishihama","doi":"10.1002/elps.70161","DOIUrl":"https://doi.org/10.1002/elps.70161","url":null,"abstract":"Low-input mass spectrometry (MS)-based proteomics is vulnerable to peptide loss during final sample preparation. We evaluated whether ChocoTip desalting and decyl maltose neopentyl glycol (DMNG)-assisted reconstitution improve peptide recovery from HeLa digests corresponding to 10 ng of protein (∼50 cells). ChocoTip reduces irreversible peptide adsorption into mesopores of chromatographic particles during microscale desalting, whereas DMNG is expected to suppress hydrophobic-interaction-mediated nonspecific adsorption to plasticware during redissolution, transfer, and injection. After selecting DMNG as the nonionic detergent additive, we compared conventional StageTip desalting, ChocoTip desalting, DMNG-assisted reconstitution, and their combination. ChocoTip and DMNG method each improved peptide recovery but affected the peptide populations eluting in different regions of the nanoflow reversed-phase liquid chromatography (nanoRPLC) gradient. Their combination yielded the highest number of peptide identifications and increased peptide intensities across almost the entire nanoRPLC gradient, including commonly identified peptides. Peptides uniquely recovered by the combined workflow covered a broad hydrophobicity range and included longer sequences. These results indicate that suppressing peptide loss at both desalting and post-desalting stages provides a simple strategy to improve sensitivity and peptide coverage in low-input MS-based proteomics.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0