ELECTROPHORESIS最新文献_第2页

Contents: Electrophoresis 7–8'25 内容：电泳7-8′25

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-29 DOI: 10.1002/elps.202570042

引用次数: 0

Editorial Board: Electrophoresis 7–8'25 编辑委员会：电泳7-8 '25

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-29 DOI: 10.1002/elps.202570041

引用次数: 0

Coupling Capillary Electrophoresis With a Shifted Inlet Potential High-Resolution Ion Mobility Spectrometer. 耦合毛细管电泳与移位进口电位高分辨率离子迁移谱仪。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-28 DOI: 10.1002/elps.8147

Klaus Welters, Christian Thoben, Christian-Robert Raddatz, Florian Schlottmann, Stefan Zimmermann, Detlev Belder

引用次数: 0

Gradient Insulator-Based Dielectrophoresis of Gold Nanoparticles. 基于梯度绝缘体的金纳米颗粒介质电泳研究。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-23 DOI: 10.1002/elps.8119

Alex J Ramirez, A K M Fazlul Karim Rasel, Sean L Seyler, Mark A Hayes

{"title":"Gradient Insulator-Based Dielectrophoresis of Gold Nanoparticles.","authors":"Alex J Ramirez, A K M Fazlul Karim Rasel, Sean L Seyler, Mark A Hayes","doi":"10.1002/elps.8119","DOIUrl":"https://doi.org/10.1002/elps.8119","url":null,"abstract":"Various forms of dielectrophoresis and higher order electrokinetic effects are being increasingly investigated and used to precisely and accurately manipulate micro and nanoparticles within microfluidic devices. The types of particles span ∼10 nm to hundreds of microns in diameter and are composed of minerals, polymers, biological materials, and complex mixtures. Some studies focused on the selective isolation and concentration of purified particles countering negative dielectrophoretic forces against flow and electrophoretic effects. Similar studies are presented here examining the behaviors of small inorganic particles (10 nm diameter) where their collective actions are inconsistent with negative dielectrophoretic effects and were consistent overall with positive dielectrophoresis (DEP). Positive DEP can account for some of the observed phenomena, particularly the deflection of large particle aggregates, which are rapidly accelerated through microchannel constrictions and then pulled back toward the constrictions against the direction of electroosmotic flow. Nevertheless, the dynamic complexity of the observed nanoparticle structures suggests that a myriad of electrostatic and possibly hydrodynamic forces, including both particle-particle and particle-device interactions, may be involved.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Controllable Cargo Delivery Vehicle Driven by Electrically Actuated Galinstan Droplets. 一种由电驱动的Galinstan液滴驱动的可控货物运输车。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-23 DOI: 10.1002/elps.8143

Qingming Hu, Fengshi Hu, Dandan Sun, Kailiang Zhang

引用次数: 0

Determination of Critical Micelle Concentration of Ionic and Non-Ionic Surfactants by Streaming Potential Measurements. 用流动电位法测定离子和非离子表面活性剂的临界胶束浓度。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-23 DOI: 10.1002/elps.8145

Yuri Chenyakin, David Da Yong Chen

{"title":"Determination of Critical Micelle Concentration of Ionic and Non-Ionic Surfactants by Streaming Potential Measurements.","authors":"Yuri Chenyakin, David Da Yong Chen","doi":"10.1002/elps.8145","DOIUrl":"https://doi.org/10.1002/elps.8145","url":null,"abstract":"A capillary electrophoresis system capable of measuring streaming potentials was used for the determination of critical micelle concentration (CMC) of anionic, cationic, zwitterionic and non-ionic surfactants. The CMC values of anionic surfactant sodium dodecyl sulphate (SDS), cationic surfactant cetyltrimethylammonium bromide (CTAB), zwitterionic surfactant 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS) and non-ionic surfactant polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether (Triton X-100) in water or salt solutions were determined by determining the abrupt change in the trend of streaming potential change with the surfactant concentration. The CMC values were 8.23, 0.93, 5.80 and 0.16 mM, respectively. This method was also used to demonstrate how the CMCs of SDS and CTAB change differently with temperature. The CMC of SDS decreased from 10°C to 25°C and then increased from 25°C to 40°C, whereas CTAB only increased linearly within 10°C-40°C. The capillary wall zeta potentials in surfactant solutions can be calculated from the measured streaming potential, conductivity and solution viscosity. Surface charge densities were calculated using the zeta potentials obtained. The surface charge densities of SDS were calculated to be 5.6-0.8 C/m2 when SDS solutions with concentrations of 2-20 mM zeta potentials were used. The calculated zeta potentials and surface charge densities reached a plateau at about 8 mM, which coincided with the CMC of SDS determined in the present study and the literature values. The CMC values obtained from streaming potential measurement are comparable to values obtained with other CMC determination techniques such as surface tension and conductometric measurements.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Label-Free Approach for Cell-Level Drug Dosage Response Tests With an Optimized Flow Cytometry Device. 利用优化的流式细胞仪进行细胞水平药物剂量反应试验的无标记方法

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-18 DOI: 10.1002/elps.8144

Junwei Li, Huan Wang, Wenjie Yang, Hailong An, Shanshan Li

{"title":"A Label-Free Approach for Cell-Level Drug Dosage Response Tests With an Optimized Flow Cytometry Device.","authors":"Junwei Li, Huan Wang, Wenjie Yang, Hailong An, Shanshan Li","doi":"10.1002/elps.8144","DOIUrl":"https://doi.org/10.1002/elps.8144","url":null,"abstract":"Cancer is among the most significant health threats to humanity. As a critical front-line treatment in the early stages of the disease, chemotherapy drugs provide positive effects on more than one disease. Traditional analytical methods for screening these drugs are often marred by the need for intricate sample preparation and reliance on costly equipment or reagents. In this study, we profiled the biophysical properties of cancer cells (MCF-7) as they traversed a detection region using a high-throughput seven-electrode double-differential biochip. To ensure precise and reliable cell status assessment, we optimized both the electrode dimensions within the assay system and the buffer's conductivities. Our findings indicated that an electrode configuration of E:F:G = 2:5:1 (E, F, and G stand for exciting/floating/gap, respectively), coupled with a conductivity setting of 1.6 S/m, was optimal for probing the electrical properties of breast cancer cells (MCF-7). Utilizing this refined system, we achieved a live-dead cell differentiation accuracy of approximately 94.25%. Moreover, MCF-7 cells displayed distinct impedance signatures in response to varying drug concentrations. Changes in impedance signal characteristics, such as opacity and phase, stand for the physiological shifts within the cells under drug exposure. This research is of considerable importance, offering a novel and efficient methodology for drug dosage response testing. It paves the way for more precise and personalized cancer treatment strategies, potentially enhancing patient outcomes and quality of life.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart Wash: Accelerated Membrane Washing Method in Immunoblot. 智能洗涤：免疫印迹加速膜洗涤方法。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-17 DOI: 10.1002/elps.8104

Ethan P Stevenson, Christopher K Schroeder, Richard Chan, Herbert M Geller, Yasuhiro Katagiri

{"title":"Smart Wash: Accelerated Membrane Washing Method in Immunoblot.","authors":"Ethan P Stevenson, Christopher K Schroeder, Richard Chan, Herbert M Geller, Yasuhiro Katagiri","doi":"10.1002/elps.8104","DOIUrl":"https://doi.org/10.1002/elps.8104","url":null,"abstract":"Immunoblot, also known as western blot, is a well-established procedure in life science. It is commonly used to determine the relative size and abundance of specific proteins, as well as posttranslational modifications of proteins. While this method is widely employed due to its simplicity, it can take hours or even days to complete. Despite considerable efforts to reduce the overall procedure time, particularly for antibody incubation, the steps involving membrane rinsing have remained unchanged since the development of the immunoblot technique. In this context, we introduce an innovative device called the \"Smart Wash,\" designed to significantly reduce the washing intervals by utilizing a motorized salad spinner. The principle of Smart Wash is akin to that of a household washing machine: the container holds the membranes during the rinsing cycle, and the basket moves the membranes along with the washing solution in the container. We have optimized the rinsing conditions, including the volume of the washing solution, rotation speed, number of washing cycles, and direction. This straightforward device empowers researchers to significantly enhance the efficiency and productivity of immunoblotting analysis.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Taurocholic Acid Binding With Insulin for Potential Oral Formulation Using Different Methods 不同方法表征牛磺胆酸与潜在口服制剂胰岛素的结合

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-16 DOI: 10.1002/elps.8139

Chang Sun, Shuanghao Wang, Huihui Li, David Da Yong Chen

{"title":"Characterization of Taurocholic Acid Binding With Insulin for Potential Oral Formulation Using Different Methods","authors":"Chang Sun, Shuanghao Wang, Huihui Li, David Da Yong Chen","doi":"10.1002/elps.8139","DOIUrl":"https://doi.org/10.1002/elps.8139","url":null,"abstract":"In diabetes management, oral formulation of insulin (INS) has the potential to improve safety, convenience, and patient-centered care compared to subcutaneous injections. However, its bioavailability remains limited, necessitating improved delivery strategies. Recent clinical trials indicate that taurocholic acid (TCA) can enhance the bioavailability of oral INS as an absorption enhancer. In this work, electrospray ionization mass spectrometry (ESI-MS) analysis revealed the formation of 1:1–1:4 INS–TCA complexes. MS/MS was used to explore the fragmentation pathway of complex ions and confirm binding stability in the gas phase. Circular dichroism spectra showed no clear conformational change in INS upon TCA binding, even though TCA enhanced INS's structural stability. Using Taylor dispersion analysis (TDA), we determined the diffusion coefficient and hydrodynamic radius of INS and its complexes. TCA binding was observed to increase INS size in both the 1:1 and 1:2 INS–TCA complexes. The binding constant of INS and TCA (1.3 × 103 L/mol) with approximately five binding sites was obtained via pressure-assisted capillary electrophoresis frontal analysis. Molecular docking simulations indicated that TCA binds to external binding sites on the INS B chain (near Ser-B9, Glu-B13, and Phe-B24 residues), consistent with ESI-MS and TDA results. These findings suggest that TCA binding may enhance INS absorption and increase the bioavailability of oral INS therapy.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 7-8","pages":"468-477"},"PeriodicalIF":3.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.8139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postulations for the Migration Behavior of Amino Acids as Cations in Capillary Zone Electrophoresis. 毛细管区带电泳中氨基酸作为阳离子迁移行为的假设。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2025-04-09 DOI: 10.1002/elps.202400205

Peter Gross, Tom Huber, Isabel Lunow, Dominik Burkhard, Holger Seelert, Rolf Müller

{"title":"Postulations for the Migration Behavior of Amino Acids as Cations in Capillary Zone Electrophoresis.","authors":"Peter Gross, Tom Huber, Isabel Lunow, Dominik Burkhard, Holger Seelert, Rolf Müller","doi":"10.1002/elps.202400205","DOIUrl":"https://doi.org/10.1002/elps.202400205","url":null,"abstract":"Amino acids (AAs) in their cationic form at pH 2.2 and usual ionic strength show a non-intuitive migration order in CZE. This is explained by setting up four postulates. The central points in these postulates are the influence of the AA side chain on the <math> <semantics><mrow><mi>p</mi> <msub><mi>K</mi> <mi>a</mi></msub> </mrow> <annotation>${rm p}K_{rm a}$</annotation></semantics> </math> value and the adoption of a defined, preferred conformation to build up the different <math> <semantics><mrow><mi>p</mi> <msub><mi>K</mi> <mi>a</mi></msub> </mrow> <annotation>${rm p}K_{rm a}$</annotation></semantics> </math> values. This conformation then also influences the hydrodynamic radius. The rotational orientation of an AA in the electric field aligns it, which also affects the hydrodynamic radius. Overall a special electrophoretical hydrodynamic radius is postulated and distinguished from the hydrodynamic radius, which is determined by the translational diffusion constant. With the help of the four postulates, the migration order could be explained. Glutamic acid has a special feature in this study: due to its observed higher mobility than the smaller and even higher charged aspartic acid, the hypothesis is that it would deprotonate first at the C5 and not at the C1 carboxylic group as all other AAs. This has the consequence of a more streamlined conformation and by that a faster migration in capillary electrophoresis.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0