AC Insulator-Based Dielectrophoretic Separation of Live and Dead Yeast Cells.

IF 2.5 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-09-18 DOI:10.1002/elps.70033

Seyed Mojtaba Tabarhoseini, Walter Johnson, Peter Michael Koniers, Tzuen-Rong Tzeng, Hui Zhao, Xiangchun Xuan

引用次数: 0

Abstract

The separation of live and dead cells is crucial for the diagnosis of early-stage diseases and efficacy test of drug screening, etc. We demonstrate the biological application of our recently developed AC insulator-based dielectrophoresis (AC iDEP) technique for the separation of live and dead yeast cells in a virtually infinitely long ratchet microchannel. This separation arises from the variation of surface charge and in turn electrokinetic velocity when yeast cells lose viability, as compared to the varying dielectrophoretic responses in conventional dielectrophoretic methods. The live and dead yeast cells can be focused toward the channel centerline and ratchet base, respectively, under AC voltages within a selected frequency and amplitude window. The performance of this cell viability-based AC iDEP separation is evaluated using the separation efficiency, which is reasonably predicted by a numerical model.

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基于交流绝缘子的活酵母细胞与死酵母细胞介电泳分离。

活细胞与死细胞的分离对早期疾病的诊断、药物筛选的疗效检验等具有重要意义。我们展示了我们最近开发的基于交流绝缘体的介质电泳（AC iDEP）技术在一个几乎无限长的棘轮微通道中分离活的和死的酵母细胞的生物学应用。这种分离源于酵母细胞失去活力时表面电荷的变化和反过来的电动速度，与传统介电方法中变化的介电反应相比。在选定的频率和幅度窗口内，在交流电压下，活的和死的酵母细胞可以分别聚焦到通道中心线和棘轮基部。利用分离效率对基于电池活力的交流iDEP分离性能进行了评价，并通过数值模型对分离效率进行了合理预测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.