ELECTROPHORESIS最新文献_第4页

Front Cover: A dual-reference study design for understanding and improving AAV genome size analysis 封面：了解和改进 AAV 基因组大小分析的双重参考研究设计

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-27 DOI: 10.1002/elps.202470091

Yali Sun, Zhi-xiang Lu, Michael Miller, Ying Valcour, Anis H Khimani, Jana Bauer, Michael Salomon, Yanhong Tong

引用次数: 0

Moment analysis method for the determination of permeation kinetics of coumarin at lipid bilayers of liposomes by using capillary electrophoresis. 利用毛细管电泳测定香豆素在脂质体双分子层渗透动力学的矩分析方法。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-27 DOI: 10.1002/elps.202400100

Kanji Miyabe, Mio Sakai, Shunta Inaba

{"title":"Moment analysis method for the determination of permeation kinetics of coumarin at lipid bilayers of liposomes by using capillary electrophoresis.","authors":"Kanji Miyabe, Mio Sakai, Shunta Inaba","doi":"10.1002/elps.202400100","DOIUrl":"https://doi.org/10.1002/elps.202400100","url":null,"abstract":"A method was developed for studying mass transfer kinetics at lipid bilayers of liposomes. Elution peaks of coumarin were measured by liposome electrokinetic chromatography (LEKC). Four types of phospholipids having different alkyl chains were used for preparing liposomes, which were used as pseudo-stationary phases in LEKC systems. Rate constants of permeation across lipid bilayers of liposomes or of adsorption at lipid membranes were determined by analyzing the first absolute and second central moments of the elution peaks measured by LEKC. The rate constants of permeation or adsorption tend to decrease with an increase in the carbon number of the alkyl chains of phospholipids. It was demonstrated that the moment analysis of elution peak profiles measured by LEKC is effective for determining lipid membrane permeability or adsorption kinetics. Compared with other conventional techniques, the method has some advantages for studying mass transfer kinetics at lipid bilayers. Solute permeation across or solute adsorption at real lipid bilayers of liposomes is analyzed. The principle of the method is the analysis of separation behavior in LEKC, which is different from that of the other ones. It is expected that the method contributes to the kinetic study of mass transfer at lipid bilayers from various perspectives.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contents: Electrophoresis 17–18'24 内容：电泳 17-18'24

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-27 DOI: 10.1002/elps.202470093

引用次数: 0

Comparative investigation of exosome extraction from rat bone marrow mesenchymal stem cells using three different methodologies. 使用三种不同方法从大鼠骨髓间充质干细胞中提取外泌体的比较研究。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-23 DOI: 10.1002/elps.202400055

Na Wang, Mingyue Yin, Jiaqi Yu, Jing Zhang, Xueli Pan

{"title":"Comparative investigation of exosome extraction from rat bone marrow mesenchymal stem cells using three different methodologies.","authors":"Na Wang, Mingyue Yin, Jiaqi Yu, Jing Zhang, Xueli Pan","doi":"10.1002/elps.202400055","DOIUrl":"https://doi.org/10.1002/elps.202400055","url":null,"abstract":"Exosomes have been identified as crucial mediators in numerous physiological and pathological processes, emerging as a focal point of scientific inquiry. This study aims to compare three methods for isolating exosomes from rat bone marrow mesenchymal stem cells: ultracentrifugation (UC), ultrafast separation system (EXODUS), and commercial precipitation kit (EXO-kit). First, the investigation compared exosomal morphology, particle size distribution, and expression of marker proteins. Subsequently, the RNA content, protein concentration, and purity of exosomes were evaluated. Finally, the impact of these exosomes on cellular metabolic viability and migration capacity was assessed. Results indicated that exosomes exhibited spherical or elliptical membrane structures, and most of the exosomes extracted by the three methods were in the range of 30to 200 nm. UC-extracted exosomes demonstrated the least impurities and clearest background, followed by EXODUS-extracted exosomes, and lastly EXO-kit-extracted exosomes. The EXO-kit-extracted exosomes yielded the highest RNA and protein content, whereas those isolated through UC exhibited superior purity. Furthermore, exosomes extracted from EXODUS and EXO-kit methods effectively enhanced the metabolic viability and migratory ability of osteoblast precursor cells compared to UC-extracted exosomes. In conclusion, each of the three methodologies presents advantages and limitations. Therefore, the selection of an appropriate exosome extraction technique should be based on specific experimental objectives and requirements.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Standardization and application of ARMS TaqMan real‐time PCR for screening of folate metabolism genes in Han Chinese ARMS TaqMan 实时 PCR 在汉族叶酸代谢基因筛查中的标准化和应用

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-17 DOI: 10.1002/elps.202400017

Peipei Deng, Xuan Liu, Yuanjing Li, Huanhuan Li, Bangrong Zhao, Shusong Wang, Jing Ma

{"title":"Standardization and application of ARMS TaqMan real‐time PCR for screening of folate metabolism genes in Han Chinese","authors":"Peipei Deng, Xuan Liu, Yuanjing Li, Huanhuan Li, Bangrong Zhao, Shusong Wang, Jing Ma","doi":"10.1002/elps.202400017","DOIUrl":"https://doi.org/10.1002/elps.202400017","url":null,"abstract":"Folate has antioxidant properties, and low concentration in seminal plasma may be associated with increased DNA damage in sperm. Mutations of the methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) genes, including MTHFR C677T (rs1801133), MTHFR A1298C (rs1801131), and MTRR A66G (rs1801394), can lead to decreased activity of the encoded folate metabolic enzymes, thereby affecting male reproduction. The current SNP detection methods commonly used in clinical practice have some shortcomings, such as long time‐consuming, complex detection steps, or high cost. The purpose of this study was to establish a simple, time‐saving, sensitive, accurate, and easy to clinical popularization method for folate metabolism gene detection. We combined ARMS‐PCR with TaqMan fluorescent probe to establish an ARMS TaqMan real‐time PCR detection method. According to the variation of rs1801131, rs1801133, and rs1801394, two specific primers (one wild type and one mutant) were designed. Mismatched nucleotides were introduced at the penultimate or third position to improve the specificity of the primer. Specific TaqMan probe was introduced to detect PCR products to improve the sensitivity of the method. The results showed that the sensitivity of ARMS TaqMan real‐time PCR in SNP genotyping was 1 ng, and the accuracy was 100%. A total of 249 clinical samples were detected by the established method, and the correlation between three SNPs and semen quality was analyzed. We found that individuals carrying the AG + GG genotype of rs1801394 had a lower risk of abnormal semen quality. In conclusion, we developed a highly sensitive, accurate, rapid, and easy to be popularized method for detecting SNPs of rs1801394, rs1801131, and rs1801133. ARMS TaqMan real‐time PCR is a reliable SNP genotyping method in folate metabolism genes.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of alkyl microporous organic network‐based capillary column for an efficient gas chromatographic separation of position isomers 制备用于高效气相色谱分离位置异构体的烷基微孔有机网络毛细管柱

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-17 DOI: 10.1002/elps.202400111

Zhi‐Yong Gao, Xi‐Jin Li, Yuan‐Yuan Cui, Cheng‐Xiong Yang

{"title":"Preparation of alkyl microporous organic network‐based capillary column for an efficient gas chromatographic separation of position isomers","authors":"Zhi‐Yong Gao, Xi‐Jin Li, Yuan‐Yuan Cui, Cheng‐Xiong Yang","doi":"10.1002/elps.202400111","DOIUrl":"https://doi.org/10.1002/elps.202400111","url":null,"abstract":"The large surface area, excellent thermal stability and easy modification make microporous organic networks (MONs) good candidates in the field of gas chromatography (GC). Due to the limited species and highly conjugated networks of MONs, their applications are still in infancy and restricted. To accelerate their developments and to enrich their types in GC, here we report the first example of synthesizing alkyl MON and its capillary column for GC separation of position isomers. Linear 1,8‐dibromooctane is used as the alkyl monomer instead of traditional aromatic ones to construct novel alkyl MON to decrease the inherent conjugated characteristic of MONs. The alkyl MON exhibits good thermal stability (up to 350°C), large surface area (1173 m2 g−1), and non‐polar character, allowing good resolution for alkanes, alkyl benzenes, alcohols, ketones, and diverse position isomers, including dichlorobenzene, trichlorobenzene, bromotoluene, nitrotoluene, methylbenzaldehyde, and ionone with the limits of detection (0.003 mg mL−1) and limits of quantitation of (0.10 mg mL−1). The in situ growth–prepared alkyl MON column demonstrates remarkable duration time and precisions for the retention relative standard deviations, (RSDs%, intra‐day, n = 7), 0.06%–0.53% (intra‐day, n = 7), and 2.87%–10.59% (column‐to‐column, n = 3). In addition, the fabricated alkyl MON‐coated capillary column offers better resolution than three commercial GC columns for the resolution of methylbenzaldehyde, bromotoluene, and chlorotoluene isomers. This work reveals the practicability for synthesizing alkyl MONs and demonstrates their prospects for position isomers separation.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Charge variant analysis of monoclonal antibodies by CZE‐MS using a successive multiple ionic‐polymer layer coating based on diethylaminoethyl‐dextran 利用基于二乙氨基乙基葡聚糖的连续多重离子聚合物层涂层，通过 CZE-MS 对单克隆抗体进行电荷变异分析

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-17 DOI: 10.1002/elps.202400084

Alisa Höchsmann, Laura Dhellemmes, Laurent Leclercq, Hervé Cottet, Christian Neusüß

{"title":"Charge variant analysis of monoclonal antibodies by CZE‐MS using a successive multiple ionic‐polymer layer coating based on diethylaminoethyl‐dextran","authors":"Alisa Höchsmann, Laura Dhellemmes, Laurent Leclercq, Hervé Cottet, Christian Neusüß","doi":"10.1002/elps.202400084","DOIUrl":"https://doi.org/10.1002/elps.202400084","url":null,"abstract":"The characterization of the impurities of pharmaceutical monoclonal antibodies (mAbs) is crucial for their function and safety. Capillary zone electrophoresis (CZE) is one of the most efficient tools to separate charge variants of mAbs; however, peak characterization remains difficult, since the hereby used background electrolytes (BGEs) are not compatible with electrospray ionization‐mass spectrometry (ESI‐MS). Here, a method that allows the separation of intact mAb charge variants is presented using CZE‐ESI‐MS, combining a cationic capillary coating and an acidic BGE. Therefore, a successive multiple ionic‐polymer layer coating was developed based on diethylaminoethyl‐dextran–poly(sodium styrene sulfonate). This coating leads to a relatively low reversed electroosmotic flow (EOF) with an absolute mobility slightly higher than that of antibodies, enabling the separation of variants with slightly different mobilities. The potential of the coating is demonstrated using USP mAb003, where it was possible to separate C‐terminal lysine variants from the main form, as well as several acidic variants and monoglycosylated mAb forms. The presented CZE‐MS method can be applied to separate charge variants of a range of other antibodies such as infliximab, NISTmAB (Reference Material from the National Institute of Standards and Technology), adalimumab, and trastuzumab, demonstrating the general applicability for the separation of proteoforms of mAbs.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streaming electric field, electroviscous effect, and electrokinetic liquid flows in the induced pressure‐driven transport of active liquids in narrow capillaries 窄毛细管中活性液体的诱导压力驱动输送中的流动电场、电粘性效应和电动液体流

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-17 DOI: 10.1002/elps.202400133

Siddhartha Das, Ghansham Rajendrasingh Chandel

{"title":"Streaming electric field, electroviscous effect, and electrokinetic liquid flows in the induced pressure‐driven transport of active liquids in narrow capillaries","authors":"Siddhartha Das, Ghansham Rajendrasingh Chandel","doi":"10.1002/elps.202400133","DOIUrl":"https://doi.org/10.1002/elps.202400133","url":null,"abstract":"In this paper, we develop a theory for studying the electrokinetic effects in a charged nanocapillary filled with active liquid. The active particles present within the active liquid are self‐driven, demonstrate vortex defects, and enforce a circumferentially arranged polarization field. Under such circumstances, there is the development of an induced pressure‐gradient‐driven transport dictated (similar to diffusioosmotic transport) by the presence of an axial gradient in the activity (or the concentration of the active particles). This pressure‐driven transport has a profile different from the standard Hagen–Poiseuille flow in a nanocapillary. Also, this induced pressure‐driven flow drives electrokinetic effects, which are characterized by the generation of a streaming electric field, associated electroosmotic (EOS) transport opposing pressure‐driven flow, and electroviscous effect. We quantify these effects as functions of dimensionless parameters that vary inversely as the strength of the activity‐induced pressure‐driven flow and salt concentrations. Overall, we anticipate that this paper will draw immense attention toward a new type of activity‐induced pressure‐driven flow and associated electrokinetic phenomena in charged nanoconfinements.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophoresis of polyelectrolyte‐adsorbed soft particle with hydrophobic inner core 具有疏水内核的聚电解质吸附软颗粒的电泳技术

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-17 DOI: 10.1002/elps.202400143

Asim Mahata, Sanjib Kumar Pal, Hiroyuki Ohshima, Partha P. Gopmandal

{"title":"Electrophoresis of polyelectrolyte‐adsorbed soft particle with hydrophobic inner core","authors":"Asim Mahata, Sanjib Kumar Pal, Hiroyuki Ohshima, Partha P. Gopmandal","doi":"10.1002/elps.202400143","DOIUrl":"https://doi.org/10.1002/elps.202400143","url":null,"abstract":"This article deals with the electrophoresis of hydrophobic colloids absorbed by a layer of polymers with an exponential distribution of the polymer segments. The functional groups present in the polymer layer further follow the exponential distribution. We made an extensive mathematical study of the electrophoresis of such core‐shell structured soft particles considering the combined impact of heterogeneity in polymer segment distribution, ion steric effect, and hydrodynamic slippage of the inner core. The mathematical model is based on the flat‐plate formalism and deduced numerical results for electrophoretic mobility are valid for weak to highly charged particles for which the particle size well exceeds the Debye‐layer thickness. In addition, we have derived closed form analytical results for electrophoretic mobility of the particle under several electrohydrodynamic limits. We have further illustrated the results for electrophoretic mobility considering a charged and hydrophobic inner core coated with an uncharged polymer layer or a polymer layer that entraps either positive or negatively charged functional groups. The impact of pertinent parameters on the overall electrophoretic motion is further illustrated.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrokinetic particle trapping in microfluidic wells using conductive nanofiber mats. 利用导电纳米纤维毡在微流孔中进行电动粒子捕集。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-02 DOI: 10.1002/elps.202400051

J Hunter West, Tonoy K Mondal, Stuart J Williams

引用次数: 0