ELECTROPHORESIS最新文献

{"title":"Electroextraction of Large Volume Samples Using Paper Points Coupled With Hollow Fiber Membranes: Study of Parameters and Strategies to Enhance Analytical Performance.","authors":"Ricardo Mathias Orlando, Miloš Dvořák, Pavel Kubáň","doi":"10.1002/elps.202400102","DOIUrl":"https://doi.org/10.1002/elps.202400102","url":null,"abstract":"<p><p>Electroextraction (EE) encompasses a range of sample preparation methods whose effectiveness, selectivity, and efficiency are significantly influenced by the physical-chemical characteristics of analytes, samples, and instrumental conditions. This article explores, for the first time, various strategies aimed at enhancing the extraction efficiency of a recent approach of EE utilizing a paper point (PP) combined with a hollow fiber (HF) (abbreviated as PP-HF-EE) to extract various cationic and anionic model compounds from water samples. The study also explores, experimentally, the impact of agitation, organic filter composition, PP diameter, and PP brand on extraction performance, and proves that all these factors are quite important, especially when digital image analysis is utilized for determination. Furthermore, this work demonstrates the ease and feasibility of simultaneously extracting cations and anions using PP-HF-EE and proposes a straightforward method to enhance analyte concentration on the vertex of the PP through a base-to-vertex focusing. Lastly, it is demonstrated, using capillary electrophoresis coupled to a UV-Vis detector, that for PP-HF-EE, the extraction efficiency and pre-concentration factor are less dependent on other parameters when multiple PPs per sample are utilized, with signal enhancement values of up to 111 and 339 for nortriptyline and haloperidol, respectively. All the findings and strategies presented herein constitute significant contributions that can facilitate future research in method development, particularly in the utilization of PP-HF-EE and similar EE approaches.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaling Effects of the Weissenberg Number in Electrokinetic Oldroyd-B Fluid Flow Within a Microchannel.","authors":"Satwik Mukherjee, Sanjib Kr Pal, Partha P Gopmandal, Sankar Sarkar","doi":"10.1002/elps.202400175","DOIUrl":"https://doi.org/10.1002/elps.202400175","url":null,"abstract":"&lt;p&gt;&lt;p&gt;This study attempts to extend previous research on electrokinetic turbulence (EKT) in Oldroyd-B fluid by investigating the relationship between the Weissenberg number ( &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;W&lt;/mi&gt; &lt;mi&gt;i&lt;/mi&gt;&lt;/mrow&gt; &lt;annotation&gt;$Wi$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) and the second-order velocity structure function ( &lt;math&gt; &lt;semantics&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt; &lt;mi&gt;v&lt;/mi&gt; &lt;mn&gt;2&lt;/mn&gt;&lt;/msubsup&gt; &lt;annotation&gt;$S_v^2$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) under applied electric fields. Inspired by Sasmal's demonstration in Sasmal (2022) of how heterogeneous zeta potentials induce turbulence above a critical &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;W&lt;/mi&gt; &lt;mi&gt;i&lt;/mi&gt;&lt;/mrow&gt; &lt;annotation&gt;$Wi$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , we develop a mathematical framework linking &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;W&lt;/mi&gt; &lt;mi&gt;i&lt;/mi&gt;&lt;/mrow&gt; &lt;annotation&gt;$Wi$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; to turbulent phenomena. Our analysis incorporates recent findings on AC (Zhao & Wang, 2017) and DC (Zhao & Wang 2019) EKT, which have defined scaling laws for velocity and scalar structure functions in the forced cascade region. Our finding shows that &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt; &lt;mi&gt;v&lt;/mi&gt; &lt;mn&gt;2&lt;/mn&gt;&lt;/msubsup&gt; &lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt; &lt;mi&gt;l&lt;/mi&gt; &lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt; &lt;mo&gt;∼&lt;/mo&gt; &lt;msubsup&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt; &lt;mrow&gt;&lt;mn&gt;4&lt;/mn&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;msup&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;mrow&gt;&lt;mn&gt;2&lt;/mn&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt; &lt;/msup&gt; &lt;/mrow&gt; &lt;annotation&gt;$S_v^2(l) sim lambda _1^{4/5} l^{2/5}$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; and &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt; &lt;mi&gt;σ&lt;/mi&gt; &lt;mn&gt;2&lt;/mn&gt;&lt;/msubsup&gt; &lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt; &lt;mi&gt;l&lt;/mi&gt; &lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt; &lt;mo&gt;∼&lt;/mo&gt; &lt;msubsup&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt; &lt;mrow&gt;&lt;mo&gt;-&lt;/mo&gt; &lt;mn&gt;2&lt;/mn&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;msup&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;mrow&gt;&lt;mn&gt;4&lt;/mn&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt; &lt;/msup&gt; &lt;/mrow&gt; &lt;annotation&gt;$S_sigma ^2(l) sim lambda _1^{-2/5} l^{4/5}$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , for a length scale &lt;math&gt;&lt;semantics&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;annotation&gt;$l$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , and &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;W&lt;/mi&gt; &lt;mi&gt;i&lt;/mi&gt; &lt;mo&gt;=&lt;/mo&gt; &lt;mfrac&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt; &lt;msub&gt;&lt;mi&gt;u&lt;/mi&gt; &lt;mi&gt;l&lt;/mi&gt;&lt;/msub&gt; &lt;/mrow&gt; &lt;mi&gt;l&lt;/mi&gt;&lt;/mfrac&gt; &lt;/mrow&gt; &lt;annotation&gt;$Wi = frac{lambda _1 u_l}{l}$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , where &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;u&lt;/mi&gt; &lt;mi&gt;l&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;=&lt;/mo&gt; &lt;msqrt&gt; &lt;mrow&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt; &lt;mi&gt;u&lt;/mi&gt; &lt;mn&gt;2&lt;/mn&gt;&lt;/msubsup&gt; &lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt; &lt;mi&gt;l&lt;/mi&gt; &lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt; &lt;/mrow&gt; &lt;/msqrt&gt; &lt;/mrow&gt; &lt;annotation&gt;$u_l = sqrt {S_u^2(l)}$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; is a velocity fluctuations quantity and &lt;math&gt; &lt;semantics&gt;&lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt; &lt;annotation&gt;$lambda _1$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; denotes the time relaxation parameter. This work establishes a positive correlation between &lt;math&gt; &lt;semantics&gt;&lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt; &lt;annotation&gt;$lambda _1$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; and turbulent flow phenomena through a rigorous analysis of velocity structure functions, thereby offe","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressurized liquid extraction of sulfite for quantitation by capillary zone electrophoresis.","authors":"Samantha Gonçalves, Luciano Molognoni, Heitor Daguer, Ana Paula Fabichak Ribeiro, Carolina Turnes Pasini Deolindo, Luciano Vitali, Rodrigo Hoff","doi":"10.1002/elps.202400115","DOIUrl":"10.1002/elps.202400115","url":null,"abstract":"<p><p>Sulfite is an additive used in shrimp processing to prevent discoloration. However, sulfite can cause health issues for sensitive consumers, making its monitoring necessary. Determining sulfite concentrations is complex because the Monier-Williams reference method is laborious and has low analytical throughput. Additionally, new techniques are needed to stabilize sulfite during the extraction process because this analyte undergoes rapid oxidation. A new method involving extraction and derivatization of sulfite with formaldehyde through automated pressurized liquid extraction (PLE), followed by quantitation by capillary zone electrophoresis with diode-array detector in indirect mode, was developed and optimized using multivariate planning. The PLE procedure was compared to another solid-liquid extraction method. The new method successfully stabilized and extracted sulfite from shrimp in few steps with adequate precision (CV < 3.8%), producing extracts that were stable for 10 days. Recovery was satisfactory (97%-99%), and the limits of detection (4.6 mg kg^-1) and quantitation (15.4 mg kg^-1) were suitable for the intended purpose.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special issue on \"Foodomics and Advanced Food Analysis,\" Autumn 2024.","authors":"Alejandro Cifuentes, Miguel Herrero","doi":"10.1002/elps.202400222","DOIUrl":"https://doi.org/10.1002/elps.202400222","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method Validation for the Determination of Ciprofloxacin in Lucilia sericata Larvae via Capillary Electrophoresis Combined With Mass Spectrometry","authors":"Magdalena Czuma-Pokusa,&nbsp;Maria Walczak","doi":"10.1002/elps.202400118","DOIUrl":"10.1002/elps.202400118","url":null,"abstract":"<div>\u0000 \u0000 <p>Substances derived from insects can serve therapeutic functions due to their diverse biological properties. This article focuses on the species <i>Lucilia sericata</i> and the benefits of larval therapy in patients who, due to hospitalization, have developed pressure ulcers and other difficult-to-heal wounds. Larval therapy, also known as maggot debridement therapy, employs sterile fly larvae to treat chronic, non-healing wounds by enzymatically degrading necrotic tissue and decreasing bacterial colonization. The larvae are applied to the wound for a period of 48–72 h, during which they effectively clean the wound and stimulate tissue regeneration. This therapeutic approach is particularly efficacious for recalcitrant wounds, such as diabetic foot ulcers and pressure sores, which have not responded to conventional treatments. Larvae may also constitute an alternative material in entomotoxicological studies to detect substances ingested at not only toxic but also therapeutic doses. The present work describes a method for assaying ciprofloxacin in <i>L. sericata</i> larvae using capillary electrophoresis coupled to mass spectrometry. The developed method features high sensitivity with a limit of quantification of 100 ± 0.018 ng/mL, as well as accuracy and precision estimated within 87%–103% and 1%–4%, respectively. An application of a simple and fast precipitation of proteins procedure for sample cleaning resulted in a highly satisfactory recovery of the analyte (90%–104%). The method was linear in a range of 100–1000 ng/mL with a determination coefficient higher than 0.9973. The method was used to determine ciprofloxacin in larval homogenate after antibiotic administration to the patient at a dose of 500 mg twice daily per os during application of the larvae dressing. Ciprofloxacin was shown to distribute from the patient's circulation to the larvae at a concentration of 150 ng/mL (750 ng/g).</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 21-22","pages":"1939-1945"},"PeriodicalIF":3.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Applications of CE-ICP-MS/MS: Monitoring of Antiaging GHK–Cu Cosmetic Component Encapsulation in Liposomes","authors":"Joanna Zajda,&nbsp;Emilia Wadych,&nbsp;Karolina Ogórek,&nbsp;Marcin Drozd,&nbsp;Magdalena Matczuk","doi":"10.1002/elps.202400047","DOIUrl":"10.1002/elps.202400047","url":null,"abstract":"<div>\u0000 \u0000 <p>The hyphenation of the separation technique with the high-sensitive mass spectrometry detection is one of the driving forces of modern analysis enabling measurements in complex matrices. In particular, capillary electrophoresis coupled to inductively coupled plasma tandem mass spectrometry allows for speciation analysis of selected analytes with a superior resolution. The mild, physiological-friendly conditions of this separation technique offer the unique advantage of analyzing chemical entities in their intact form, which has been successfully exploited in various areas. Herein, we report the pioneering application of such a hyphenated technique in the cosmetic field to investigate the encapsulation of copper tripeptide complex (GHK–Cu) in liposomes. By monitoring copper and phosphorus signals, the formation of liposomes via a simple ethanol injection method was confirmed, and the concentration of GHK–Cu in the liposomes was assessed. The application of coupling of capillary electrophoresis with inductively coupled plasma tandem mass spectrometry (CE-ICP-MS/MS) in cosmetic studies could lead to the development of diverse liposomal formulations with preferential properties and expand their accessibility.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 21-22","pages":"1946-1954"},"PeriodicalIF":3.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board: Electrophoresis 19–20'24","authors":"","doi":"10.1002/elps.202470102","DOIUrl":"https://doi.org/10.1002/elps.202470102","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 19-20","pages":"1659"},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202470102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142555359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contents: Electrophoresis 19–20'24","authors":"","doi":"10.1002/elps.202470103","DOIUrl":"https://doi.org/10.1002/elps.202470103","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 19-20","pages":"1660-1662"},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142555360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: A convenient guideline to determine if two Y-STR profiles are from the same lineage.","authors":"","doi":"10.1002/elps.8055","DOIUrl":"https://doi.org/10.1002/elps.8055","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of Probabilistic Genotyping Method for Combining Evidence Across Microhaplotype DNA Mixture Profiles.","authors":"Xiaohua Ling, Shuang Han, Xinyi Lin, Zhaochen Bai, Nan Zhang, Jiayue Li, Huan Wang, Xueling Ou","doi":"10.1002/elps.202400140","DOIUrl":"https://doi.org/10.1002/elps.202400140","url":null,"abstract":"<p><p>In cases of serious crimes that involve challenging DNA samples from the perpetrator (e.g., a minor contributor to a mixture), there is justification to combine different mixture profiles. In our previous study, we developed a massively parallel sequencing (MPS)-based assay targeting 140 microhaplotype markers. In this study, we extended the use of the microhaplotype panel to common scenarios, such as determining the presence of a common contributor or relatedness between different mixture profiles when no reference source is available. Data interpretation was performed using the R package KinMix. Our findings revealed that correct assignments of a common contributor and relatedness were made between relatively balanced mixtures. However, when profiles suffered from allele imbalance, inclusive assignments were significantly associated with the suspect's mixture proportion. Additionally, our analysis showed zero false-positive rates in the studied scenarios. These results indicate that microhaplotype data can be reliably interpreted for identifying a common donor or related donors among different mixtures. Further research based on larger sample sizes may yield more reliable results, which could assist in solving issues related to complex scenarios where multiple mixed profiles were involved.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}