ELECTROPHORESIS最新文献_第5页

Miniaturized nanoelectrospray interface for coupling capillary electrophoresis with mass spectrometry detection. 用于毛细管电泳与质谱检测耦合的微型纳米电喷雾接口。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-23 DOI: 10.1002/elps.202400090

Tomáš Václavek, Elizaveta Vereshchagina, Leny Nazareno, Anand Summanwar, František Foret, Roman Řemínek

引用次数: 0

Contents: Electrophoresis 15–16'24 内容：电泳 15-16'24

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-21 DOI: 10.1002/elps.202470083

引用次数: 0

Nanopore-based detection of periodontitis biomarker miR31 in saliva samples. 基于纳米孔技术检测唾液样本中的牙周炎生物标志物 miR31。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-21 DOI: 10.1002/elps.202400134

Pearl Arora, Haiyan Zheng, Sathishkumar Munusamy, Rana Jahani, Xiyun Guan

引用次数: 0

Front Cover: Using capillary electrophoresis sodium dodecyl sulfate (CE-SDS) and liquid chromatograph mass spectrometry (LC–MS) to identify glycosylated heavy chain heterogeneity in the anti-VEGFR-2 monoclonal antibody 封面：利用毛细管电泳十二烷基硫酸钠法（CE-SDS）和液相色谱质谱法（LC-MS）鉴定抗血管内皮生长因子受体-2 单克隆抗体中糖基化重链的异质性

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-21 DOI: 10.1002/elps.202470081

Meng Li, Xueyu Zhao, Gang Wu, Wenbo Wang, Jialiang Du, Gangling Xu, Maoqin Duan, Zhihao Fu, Chuanfei Yu, Lan Wang

引用次数: 0

Correction to “Analytical solutions for viscoelectric effects in electrokinetic nanochannels” 对 "电动力纳米通道中粘电效应的分析解决方案 "的更正。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-21 DOI: 10.1002/elps.202410715

引用次数: 0

Editorial Board: Electrophoresis 15–16'24 编辑委员会：电泳 15-16'24

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-21 DOI: 10.1002/elps.202470082

引用次数: 0

Investigation of sample handling steps for accurate heparan sulphate disaccharide analysis using HPLC-MS. 利用 HPLC-MS 对硫酸肝素二糖进行精确分析的样品处理步骤研究。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-21 DOI: 10.1002/elps.202400091

Domonkos Pál, Gábor Tóth, Lilla Turiák

{"title":"Investigation of sample handling steps for accurate heparan sulphate disaccharide analysis using HPLC-MS.","authors":"Domonkos Pál, Gábor Tóth, Lilla Turiák","doi":"10.1002/elps.202400091","DOIUrl":"https://doi.org/10.1002/elps.202400091","url":null,"abstract":"Heparan sulphates (HSs), a specific class of glycosaminoglycans (GAGs), are important participants of cellular signalling. Analytical characterization of GAGs requires a complex sample preparation workflow. Although a detailed stability and recovery study is available for the chondroitin sulphate GAG class, the literature concerning HS is incomplete in this regard. Therefore, our aim was to systematically investigate various parameters that could potentially influence the stability and recovery of HS samples when performing disaccharide analysis using high-performance liquid chromatography-mass spectrometry. First, effects concerning vacuum evaporation and freezing were investigated. Next, the storage stability of the HS disaccharides was analysed under several conditions such as temperature, pH, digestion buffers, injection solvents and storage vessels. We have identified several critical parameters influencing the stability and recovery of HS disaccharides. We concluded that major sample loss is expected when Tris-HCl is used as digestion buffer, followed by vacuum evaporation at elevated temperatures, or samples are stored under alkaline conditions. Following the practical considerations of this paper can contribute to increasing the reliability of future analytical measurements.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A semen-specific deoxyribonucleic acid methylation model for epigenetic age estimation and its robustness under environmental challenges 用于表观遗传年龄估计的精液特异性脱氧核糖核酸甲基化模型及其在环境挑战下的稳健性。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-20 DOI: 10.1002/elps.202400054

Sena Er, Zehra Abik, Gokhan Ersoy, Gonul Filoglu, Hamdi Ozkara, Ozlem Bulbul

{"title":"A semen-specific deoxyribonucleic acid methylation model for epigenetic age estimation and its robustness under environmental challenges","authors":"Sena Er, Zehra Abik, Gokhan Ersoy, Gonul Filoglu, Hamdi Ozkara, Ozlem Bulbul","doi":"10.1002/elps.202400054","DOIUrl":"10.1002/elps.202400054","url":null,"abstract":"In forensic investigations, semen samples are a common form of biological evidence, especially in cases involving sexual assault. Therefore, accurately estimating the age of an individual is crucial in criminal cases. This study presents a novel age estimation model based on semen-specific CpG methylation patterns. A multiplex panel was developed, consisting of 12 CpG sites (PARP14, C5orf25, cg23488376, MXRA5, PFKFB3, DLL1, NOX4, cg12837463, TTC7B, KCNA7, NKX2-1, and SYNE4), which exhibit strong correlations with age. Additionally, this study investigates the resilience of these methylation markers under simulated environmental challenges. We collected ejaculate samples from a diverse cohort of 115 male individuals, aged 20–71 years, who underwent deoxyribonucleic acid extraction and bisulfite conversion. Methylation levels of the selected CpG sites were assessed using a SNaPshot assay, which revealed significant correlations with chronological age. We developed and validated two robust age estimation models through stepwise and enter regression analyses, achieving reliable accuracy with mean absolute errors ranging from 3.81 to 4.1 years. Additionally, the study also investigated the robustness of semen stains under diverse environmental conditions, including fabric type, washing, hematin exposure, and UV-C light. The selected methylation markers demonstrated remarkable resilience despite the challenges posed by washing procedures and environmental exposure, confirming their potential for age estimation in forensic genetics. This research presents successful age estimation models, emphasizing the strong correlations between methylation levels and chronological age. The proposed methodology's accuracy is affirmed through model validation on an independent test set, while also highlighting the resilience of semen stains on fabrics under varying storage and washing conditions.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility study of a single-primer extension-based microhaplotype NGS system. 基于单引物延伸的微单体型 NGS 系统的可行性研究。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-20 DOI: 10.1002/elps.202400012

Qiang Zhu, Panyin Shu, Haoyu Wang, Yifan Wei, Yuting Wang, Yufang Wang, Ji Zhang

{"title":"Feasibility study of a single-primer extension-based microhaplotype NGS system.","authors":"Qiang Zhu, Panyin Shu, Haoyu Wang, Yifan Wei, Yuting Wang, Yufang Wang, Ji Zhang","doi":"10.1002/elps.202400012","DOIUrl":"https://doi.org/10.1002/elps.202400012","url":null,"abstract":"DNA degradation has been a thorny problem in forensic science. Shortening the amplicon length of the genetic markers improves the analysis of degraded DNA effectively. Microhaplotype (MH) has been proposed as a potential genetic marker that can be used for degraded DNA analysis. In the present study, a 146-plex MH-next-generation sequencing (NGS) system with an average Ae of 6.876 was constructed. Unlike other MH studies, a single-primer extension (SPE)-based NGS library preparation method was used to improve the detection of MH markers for degraded DNA. SPE employs a locus-specific and universal primer to amplify target fragments, reducing the necessity for complete fragment sequences. SPE might effectively mitigate the impact of degradation on amplification. However, SPE produces amplicons of varying lengths, posing challenges in allele calling for SPE-NGS data. To address this issue, this study proposed a flexible allele-calling strategy to improve amplicon detection. In addition, this study evaluated the forensic efficacy of the system using 12 low-template samples (from 1 ng to 7.8 pg), 10 mock-degraded DNA with various degrees of degradation, and 8 forensic casework samples. When the template is as low as 7.8 pg, our system can accurately detect at least 37 loci and achieves a random match probability (RMP) of 10-30 using the complete allele-calling strategy. Eighty-two loci can be detected, and RMP can reach 10-54 using a flexible allele-calling strategy. After 150 min of 98°C treatment, 36 loci can still be detected, and an RMP of 10-5 can be obtained using the flexible allele-calling strategy. Furthermore, the number of single nucleotide polymorphism detected at different DNA amounts and degradation levels suggests that the SPE method combined with a flexible allele-calling strategy is effective.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Food profiling goes green: Sustainable analysis strategies for food authentication. 食品分析走向绿色：食品认证的可持续分析策略。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-14 DOI: 10.1002/elps.202400098

Marina Creydt, Markus Fischer

引用次数: 0