ELECTROPHORESIS最新文献

{"title":"Utilization of Terephthalic Acid Obtained From Textile Waste for Environmental Detection of Mitoxantrone Using Molecularly Imprinted Electrochemical Sensor Technology.","authors":"Meysera Bakırcı, Beril S Kaya, Abdullah Al Faysal, Taner Erdoğan, Ayşegül Gölcü","doi":"10.1002/elps.70150","DOIUrl":"https://doi.org/10.1002/elps.70150","url":null,"abstract":"<p><p>The global textile industry generates substantial amounts of waste, much of which originates from polyethylene terephthalate (PET). Due to its resistance to natural degradation, PET accumulates in the environment and contributes to pollution and landfill growth. Chemical depolymerization offers a sustainable strategy for mitigating these impacts while recovering valuable monomers such as terephthalic acid (TPA) for high-value applications. In this study, five textile waste materials with varying PET content were subjected to alkaline hydrolysis using sodium hydroxide in a methanol-water medium. Pre-treated fabrics were cut into 2 mm pieces and reacted at 80°C for 10-65 min. The recovered products were isolated by acidification and subsequently characterized by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy, with the obtained spectra confirming the successful recovery of TPA and its expected molecular structure. The efficiency of TPA recovery was strongly influenced by fabric composition and reaction time. Fabric 5, containing 50% PET, produced the highest yield (27.91%) at 50 min, followed by Fabric 4 (24.77%). Fabrics 1-3 yielded between 20.61% and 23.77%, with optimum recovery occurring between 20 and 60 min. Prolonged reaction times led to reduced yields, suggesting partial degradation or re-dissolution of the product. To demonstrate value-added utilization, the recovered TPA was employed as a functional monomer in the synthesis of a molecularly imprinted polymer (MIP) for the selective electrochemical detection of the anticancer drug mitoxantrone (MTX) in tap water. Density functional theory (DFT) calculations were performed to investigate MTX-TPA interactions at template-to-monomer ratios from 1:1 to 1:5. Both computational and experimental results identified the 1:1 ratio as optimal. The developed MIP-based sensor achieved a detection limit of 2.13 × 10^-13 M. These findings demonstrate the potential of converting textile waste into high-value functional materials for advanced environmental monitoring and analytical applications.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical Signatures of Life: A Review of Cell Electrophysiology and Dielectric Analysis (2015-2026).","authors":"Alaleh Vaghef-Koodehi, Blanca H Lapizco-Encinas","doi":"10.1002/elps.70146","DOIUrl":"https://doi.org/10.1002/elps.70146","url":null,"abstract":"<p><p>The electrical characteristics of biological cells offer a powerful, label-free modality for investigating cellular morphology and phenotype, monitoring drug effects, and enabling selective manipulation and sorting strategies. Fundamental properties-including permittivity, conductivity, membrane capacitance, polarizability, and surface charge-dictate cellular responses to external electric fields, providing deep insights into cellular structure, physiology, and population heterogeneity. This review examines the recent literature on cell electrophysiology and highlights how innovations in utilizing cellular electrical signatures drive a diverse range of critical applications. Emphasis is given to the latest advances in the analysis of prokaryotic and eukaryotic systems across medical, clinical, environmental, and industrial contexts. This work aims to provide a comprehensive overview of relevant recent reports and novel platforms, where electrokinetic methodologies have been successfully applied to enable rapid cellular assessments, illustrating the potential for applying cellular electrical signatures across numerous scientific disciplines, including clinical diagnosis, drug development, personalized medicine, and industrial bioprocessing.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral/Achiral Separation of Novel Synthetic Cannabinoids in Sub/Supercritical Fluid Chromatography.","authors":"Květa Kalíková, Matúš Kapusta, Eva-Maria Hubner, Martin G Schmid","doi":"10.1002/elps.70149","DOIUrl":"https://doi.org/10.1002/elps.70149","url":null,"abstract":"<p><p>Synthetic cannabinoids (SCs) belong to one of the fastest-growing classes of new psychoactive substances nowadays. Among users, they can be sprayed onto herbal incense or paper or used as E-liquids. As many of them are still legal, they can be purchased on the internet very easily. Their continuous appearance on the illicit drug market and structural variability poses significant challenges for their analysis and detection. Moreover, some of them are chiral and could be distributed either as pure enantiomers or racemates. Sub/supercritical fluid chromatography (SFC) is regarded as a greener separation technique than normal-phase liquid chromatography. In this work, we demonstrate the potential of SFC both for enantioseparation and achiral separation of a set of 11 chiral SCs obtained from internet vendors. On the basis of the results from HPLC measurement with optical rotation detector, two samples were present as racemates, whereas the others were not. Simultaneous baseline enantioseparation of both racemates in SFC confirmed its high enantioselective potential. Moreover, an SFC method with a specialized, diol-bonded stationary phase designed primarily for achiral screening purposes was presented.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Analysis of Amino Acids and Their Derivatives in Lucilia sericata Larval Excretions/Secretions Using Capillary Electrophoresis-Mass Spectrometry.","authors":"Magdalena Czuma-Pokusa, Agnieszka Zakrzewska, Anna Kurpińska, Maria Walczak","doi":"10.1002/elps.70145","DOIUrl":"https://doi.org/10.1002/elps.70145","url":null,"abstract":"<p><p>A capillary electrophoresis-mass spectrometry (CE-MS) method was developed and validated for the determination of 19 amino acids and related compounds in excretions/secretions (ES) of Lucilia sericata larvae. Sample preparation consisted of dilution of ES with water, filtration and direct CE-MS analysis. Separation was performed with 50 mM formic acid as the background electrolyte, and methanol-water (1:1, v/v) containing 1% formic acid was used as sheath liquid. The total analysis time was 20 min. The method showed acceptable linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), sensitivity, apparent recovery and short-term applicability for freshly prepared samples. Correlation coefficients for the calibration curves ranged from 0.983 to 0.998. Precision was within 18% at the lowest quality control level and within 15% at medium and high levels. Accuracy ranged from 83% to 118% at the lowest level and from 85% to 115% at the other levels. In larval ES, the most abundant compounds were threonine (565 ± 7 µM), tryptophan (455 ± 6 µM), anserine (404 ± 7 µM), histidine (330 ± 14 µM) and cystine (279 ± 4 µM). The method offers a simple workflow for targeted profiling of amino acids and related compounds in larval ES.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148789671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Chemistry 2026","authors":"Ann Van Schepdael","doi":"10.1002/elps.70115","DOIUrl":"10.1002/elps.70115","url":null,"abstract":"<p>Green Chemistry 2026 issue, focusing on drug impurity analysis (2 reviews), biosourced preservatives (by LC and SFC), phosphate syrup (by IC), insulin and dapagliflozin (by CE).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of Heparin Translocations Through Solid-State Nanopores","authors":"Kexin Zhao,&nbsp;Matthew Waugh,&nbsp;Kyle Briggs,&nbsp;Mohammad P. Jamshidi,&nbsp;Janelle Sauvageau,&nbsp;Vincent Tabard-Cossa","doi":"10.1002/elps.70123","DOIUrl":"10.1002/elps.70123","url":null,"abstract":"<p>Carbohydrates are one of the many biomolecular building blocks of life, with many practical applications in medicine. However, current methods for analysing these biomolecules struggle to operate at the single-molecule level. Carbohydrate sensing with solid-state nanopores has recently been explored but has proved difficult due to their structural complexity and the rapid translocation through the pores in the case of short polysaccharide chains. Among the numerous forms they can take, the best-known example is heparin, a widely used anticoagulant that has a closely related but toxic contaminant. In this work, we study the kinetics of capture and translocation of heparin molecules passing through a solid-state nanopore based on experimental results and signal simulations, seeking to understand the dynamics of passage. We examine the molecular interaction with the pore as a function of pH to elucidate the conformational dynamics of translocation. Despite using state-of-the-art electronics, we find that the durations of translocation through bare silicon nitride pores are too short to provide the resolution needed to reliably distinguish heparin from its contaminants. En route, we shed light on the physical mechanisms that govern translocation and suggest practical means by which future work might overcome these limitations.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"799-808"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148276234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane","authors":"Jia Jia Seet,&nbsp;Spas D. Kolev,&nbsp;Rosanne Guijt,&nbsp;Hong Heng See,&nbsp;Fernando Maya,&nbsp;Mostafa A. Atia,&nbsp;Eddie A. Nagul,&nbsp;Owen P. Missen,&nbsp;Michael Breadmore","doi":"10.1002/elps.70120","DOIUrl":"10.1002/elps.70120","url":null,"abstract":"<p>Electrokinetic extraction (EKE), coupled with polymer inclusion membranes (PIMs) for colourimetric detection, offers a promising approach for extracting metal ions from solid matrices, such as sand, without sample pretreatment. Our EKE system consists of a formic acid gel positioned at the cathode side to neutralise hydroxide ions generated by electrolysis during the EKE of Cu(II), whereas the anode side contains the sample compartment, prepared from a syringe tube into which the sample is placed. The PIM is sandwiched between these compartments to accumulate Cu(II) via EKE through the sand and into the PIM within which it complexes with a colour-forming reagent, 1-(2-pyridylazo)-2-naphthol (PAN), enabling quantification via colourimetric analysis. The system has been optimised to expand the quantification range of Cu(II), thus allowing greater applicability to a wider range of samples with different levels of heavy metals. Increasing the column diameter extended the upper limit of the quantification range from 7.5 mg kg⁻¹ (4.5 mm column diameter) to 30.0 mg kg⁻¹ (13.5 mm column diameter). Concurrently, optimisation of the PIM composition, containing 43.3 wt% cellulose triacetate (CTA)–56.0 wt% di-(2-ethylhexyl) phosphoric acid (D2EHPA)–0.8 wt% PAN, enhanced the overall recovery of Cu(II) from 64% ± 7% to 90% ± 4%. Overall, the optimised system has achieved a quantification range of 2.8–30.0 mg kg⁻¹ of Cu(II), with an LOD of 0.9 and LOQ of 2.8 mg kg⁻¹ of Cu(II).</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"744-753"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148351146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes","authors":"Jiaming Xue,&nbsp;Qiushuo Wu,&nbsp;Mengyu Tan,&nbsp;Yazi Zheng,&nbsp;Guihong Liu,&nbsp;Haoyan Jiang,&nbsp;Yuxuan Tan,&nbsp;Yuanyuan Xiao,&nbsp;Meli Lv,&nbsp;Shengqiu Qu,&nbsp;Weibo Liang,&nbsp;Lin Zhang","doi":"10.1002/elps.70122","DOIUrl":"10.1002/elps.70122","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluated kinship identification using 19 short tandem repeats (STRs) from AGCU EX22 kit, 119 microhaplotypes (52 in Panel A + 67 in Panel B), and a theoretical 9622 genome-wide single nucleotide polymorphisms (SNPs) panel (The 9K). The system power was assessed accounting for linkage on the basis of 198 Chinese Southern Han (CHS) family samples and simulated data. STR + Panels A and B distinguished full-sibling (FS) from unrelated (un), with second-degree effectiveness above 0.96 when log₁₀(LR) threshold was 4 and low false positive rate (&lt;0.06%), but were insufficient for more distant kinship identification alone. The 9K panel was sufficient to separate third-degree from un but not for fourth-degree or more distant relatives. For discrimination among different kinships, STR + Panels A and B successfully distinguished all FS from half-siblings (HS), uncle/aunt–nephew/niece (UN), and grandparents–grandson/granddaughter (GS) in real data, with minimal overlaps (&lt;0.2%). Discriminating second- and third-degree or within second-degree relatives seems very challenging, even for 9K panel. Including a carefully selected additional relative (AR) may improve discrimination between GS and UN/HS, such as AR (GS-un, which is GS for one person and un for the other) and AR (parent/child (PC)-UN). Similarly, an AR (PC-GS) may help distinguish UN from GS/HS, but there was slightly improved efficiency for HS.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"779-787"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Flunixin Meglumine in Veterinary Pharmaceutical Formulations by Capillary Electrophoresis With Capacitively Coupled Contactless Conductivity Detection","authors":"Letícia M. de Araujo,&nbsp;José A. Fracassi da Silva,&nbsp;Dosil P. de Jesus","doi":"10.1002/elps.70114","DOIUrl":"10.1002/elps.70114","url":null,"abstract":"<p>This work describes a novel, simple, fast, reliable, and environmentally friendly analytical method for the determination of the anti-inflammatory and analgesic drug flunixin meglumine (FM) in veterinary pharmaceutical formulations. The method uses capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C⁴D) to quantify meglumine (<i>N</i>-methylglucamine), the counterion of flunixin in FM. This approach allows the use of the less expensive and more readily available meglumine, rather than FM or flunixin, as the standard reagent. Lithium ions were used as an internal standard, and the migration time of the meglumine was 2.2 min using a capillary with a total length of 50 cm (42 cm effective) and 50 µm (i.d.), filled with a background electrolyte (BGE) composed of 2-(<i>N</i>-morpholino)ethanesulfonic acid (MES) and histidine (His), each at 20 mmol L⁻¹. Good linearity was attained (<i>R</i>² = 0.992) in a concentration range of 100–2500 µg mL⁻¹. The limits of detection (LOD) and quantification (LOQ) were calculated as 22.7 and 75.6 µg mL⁻¹, respectively. The accuracy of the method was assessed using recovery tests at three concentration levels, resulting in recoveries ranging from 89% to 104%. The intra- and inter-day precisions ranged from 2.3% to 4.2%. The proposed CE-C⁴D was successfully applied to determine FM in commercial veterinary formulations.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"690-695"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148005018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening CZE–UV Green Method for Acetate, Propionate, Butyrate, and Valerate Analysis in Human Stool for Complementary Gut Dysbiosis Diagnosis","authors":"Olívia B. O. Moreira,&nbsp;Matheus Miranda Fracetti,&nbsp;Eliane B. T. Lemos,&nbsp;Jeanette C. F. Less,&nbsp;Lúcio M. Lemos,&nbsp;Paula Rocha Chellini,&nbsp;Marcone A. L. Oliveira","doi":"10.1002/elps.70117","DOIUrl":"10.1002/elps.70117","url":null,"abstract":"<p>Short-chain fatty acids present relevant function in the human body as fermentation metabolites pathways in the natural intestine microbiota activity. Their evaluations can be useful for indication as biomarkers for gut dysbiosis, provided an important health assessment to physicians by specialized laboratories. In the present work, a capillary zone electrophoresis with indirect ultraviolet (CZE–UV) detection method for simultaneous separation of acetate, propionate, butyrate, and valerate in human stool samples was performed. The background electrolyte consisted of 15 mmol L⁻¹ of tris(hydroxymethyl)aminomethane (TRIS), 12 mmol L⁻¹ of 3,5-dinitrobenzoic acid (3,5-DNB) and 0.5 mmol L⁻¹ of cetyltrimethylammonium bromide (CTAB) as buffer at pH 7.5, within run time of 7.5 min. The proposed method resulted in an accuracy of 105.5%, 90.12%, 95.9%, and 86.6% and precision of 12.2%, 8.96%, 8.84%, and 18.3% relative standard deviation (RSD) for acetate, propionate, butyrate, and valerate, respectively. The optimized CZE–UV method was successfully applied as potential complementary analytical tool for gut dysbiosis diagnostic assay at 98 stool samples from patients under investigation of intestine-related anomalies, combining eco-friendly, quickness, automated, cost-effective, and high-throughput aspects. Furthermore, the method offers educational value by exemplifying how sustainable analytical practices can be integrated into modern laboratory training and the teaching of Green Chemistry principles.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"713-723"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}