ELECTROPHORESIS最新文献_第5页

Mirror-Image β-l-Cyclodextrin as a Chiral Pseudophase in Capillary Electrophoresis 镜像β-l-环糊精作为毛细管电泳中的手性假相。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-12-16 DOI: 10.1002/elps.70061

Saba Aslani, Ryan Jacob Burk, Yong Wu, Daniel W. Armstrong

{"title":"Mirror-Image β-l-Cyclodextrin as a Chiral Pseudophase in Capillary Electrophoresis","authors":"Saba Aslani, Ryan Jacob Burk, Yong Wu, Daniel W. Armstrong","doi":"10.1002/elps.70061","DOIUrl":"10.1002/elps.70061","url":null,"abstract":"<div>\u0000 \u0000 <p>Cyclodextrins (CDs) and their derivatives are among the most versatile and effective pseudophases used for chiral capillary electrophoresis (CE). They provide a variety of interactions that could be further expanded by derivatization. Among the three native CDs used in CE, <span></span><math>\u0000 <semantics>\u0000 <mi>β</mi>\u0000 <annotation>${{beta}}$</annotation>\u0000 </semantics></math>-<span>d</span>-CD and its derivatives have been the most effective chiral selectors and have shown enantioselectivity for a variety of pharmaceutical analytes. The advent of mirror-image CDs has provided great potential in chiral CE applications. In this study, the chiral separation of 38 analytes was investigated with capillary electrophoresis and the enantiomeric selectivity was compared utilizing <span></span><math>\u0000 <semantics>\u0000 <mi>β</mi>\u0000 <annotation>${{beta}}$</annotation>\u0000 </semantics></math>-<span>d</span>-CD and <span></span><math>\u0000 <semantics>\u0000 <mi>β</mi>\u0000 <annotation>${{beta}}$</annotation>\u0000 </semantics></math>-<span>l</span>-CD as CE pseudophases. Additionally, the parameters affecting the CE separation, including pH of the run buffer, capillary voltage, and ionic strength of the buffer, were examined. For the first time, the opposite enantioselectivity of the <span></span><math>\u0000 <semantics>\u0000 <mi>β</mi>\u0000 <annotation>${{beta}}$</annotation>\u0000 </semantics></math>-<span>l</span>-CD was utilized to facilitate challenges in the quantitation of chiral impurities in pharmaceutical compounds.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"22-31"},"PeriodicalIF":2.5,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Intra- and Intercapillary Variability in HbA1c Measurement Using the Sebia Capillarys OCTA3 Analyzer 使用Sebia capillys OCTA3分析仪评估HbA1c测量的毛细管内和毛细管间变异性。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-12-14 DOI: 10.1002/elps.70064

Meriem Belhedi, Wiem Lazzem, Othman Bacha, Afif Ba, Dabboubi Rym, Sonia Chouaieb

{"title":"Assessment of Intra- and Intercapillary Variability in HbA1c Measurement Using the Sebia Capillarys OCTA3 Analyzer","authors":"Meriem Belhedi, Wiem Lazzem, Othman Bacha, Afif Ba, Dabboubi Rym, Sonia Chouaieb","doi":"10.1002/elps.70064","DOIUrl":"10.1002/elps.70064","url":null,"abstract":"<div>\u0000 \u0000 <p>Hemoglobin A1c (HbA1c) measurement is crucial for diabetes monitoring. Validation of analytical methods ensures the reliability of results. We verified the analytical performance of HbA1c measurement in each capillary of the Capillarys OCTA3 automated capillary analyzer, following the French accreditation committee guide. For each capillary and each HbA1c level, we evaluated repeatability, intermediate precision, accuracy, robustness, and measurement uncertainty. Results were compared to acceptance limits from French Society of Clinical Biology (SFBC), European Federation of Clinical Chemistry and Laboratory Medicine (EFLM), Réseau d'identification et de Communication en Oncologie et Sénologie (RICOS), and Clinical Laboratory Improvement Amendments (CLIA). A comparison with the high-performance liquid chromatography (HPLC) method was performed on 37 samples using linear regression and Bland–Altman analysis. Repeatability coefficient of variations (CVs) were less than 1.1% for both levels, consistent with supplier and SFBC (3.8%) thresholds. Precision CVs were 1.24% (normal) and 1.3% (pathological), below SFBC and supplier limits but above the strict EFLM standard. Accuracy showed consistent biases between 0% and 1.02%. Method comparison showed acceptable agreement. Expanded uncertainties were 3.10% (normal) and 2.89% (pathological), exceeding EFLM recommendations (1.8%). Westgard and CLIA sigma indices confirmed satisfactory robustness; EFLM indices were lower for some capillaries, limiting Six Sigma use. The OCTA3 analyzer demonstrated excellent analytical performance. Individual capillary verification yielded satisfactory results. Despite uncertainty exceeding EFLM recommendations and variable robustness, this method is reliable for routine clinical use.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"32-42"},"PeriodicalIF":2.5,"publicationDate":"2025-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green Analytical Techniques for Impurity Determination in Pharmaceuticals 药品中杂质测定的绿色分析技术。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-12-06 DOI: 10.1002/elps.70062

Muneeb Ur Rahman, Habiba Akram, Mubashra Saeed, Erwin Adams

{"title":"Green Analytical Techniques for Impurity Determination in Pharmaceuticals","authors":"Muneeb Ur Rahman, Habiba Akram, Mubashra Saeed, Erwin Adams","doi":"10.1002/elps.70062","DOIUrl":"10.1002/elps.70062","url":null,"abstract":"<div>\u0000 \u0000 <p>Green analytical chemistry (GAC) aims to achieve faster, safer, accurate, and sustainable analysis by minimizing the hazardous impact of organic solvents on the environment. Here, the focus will be on impurity profiling of pharmaceuticals. This can be done by employing green chromatographic techniques using eco-friendly solvents, advanced spectroscopic approaches, microextraction, and miniaturization techniques. Pharmaceutical analysis's greenness can be evaluated using various assessment tools and the advanced analytical greenness metric (AGREE). This review comparatively discusses all these tools by highlighting their eco-friendly nature, methodology, adherence to the 12 major principles of green chemistry, and validation of their results. Furthermore, state-of-the-art case studies utilizing green chemistry principles for impurity profiling of pharmaceuticals have also been discussed, along with their employed techniques and observations, thus offering insights into successful implementation. Still, cost, affordability, scalability, and reproducibility vary across techniques, with, for example, supercritical fluid chromatography (SFC) showing more substantial potential than nuclear magnetic resonance (NMR). Greenness metrics also lack global harmonization, limiting comparability. Challenges like stringent regulatory frameworks and a lack of standardized global policies should be overcome by integrating artificial intelligence (AI) and machine learning (ML), as well as more interdisciplinary collaboration among associated professionals, for the widespread adoption of efficient, sustainable, and eco-friendlier pharmaceutical analysis techniques. For AI/ML, trustworthiness will require validation and regulatory alignment to ensure they complement rather than replace existing protocols.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"87-105"},"PeriodicalIF":2.5,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145687132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning Apparent Peak Efficiency in Capillary Electrophoresis Using Backscatter Interferometry Detection 用后向散射干涉法检测毛细管电泳的视峰效率。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-12-06 DOI: 10.1002/elps.70059

Miyuru De Silva, Stanslaus M. Kariuki, Robert C. Dunn

{"title":"Tuning Apparent Peak Efficiency in Capillary Electrophoresis Using Backscatter Interferometry Detection","authors":"Miyuru De Silva, Stanslaus M. Kariuki, Robert C. Dunn","doi":"10.1002/elps.70059","DOIUrl":"10.1002/elps.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>Backscatter interferometry (BSI) is a refractive index detection method for capillary electrophoresis that is inexpensive, flexible, and easily miniaturized. Interestingly, unlike most detectors that respond exclusively to analyte concentration, the BSI signal is sensitive to both refractive index (analyte concentration) and the separation voltage. The latter is linked to zone conductivity and leads to improved BSI signals and lower detection limits with increasing field strengths. Enhanced BSI signals can also be generated using a photothermal mechanism, where resonantly excited analytes release heat into their surroundings to increase the BSI signal amplitude. Both voltage-based and photothermal signal enhancement mechanisms can lead to a change in the polarity of the BSI signal, which can be either positive or negative depending on the specific analyte, its concentration, and the separation conditions. Here, we show that this leads to a significant increase in apparent peak efficiency. At the transition in peak polarity, both mechanisms result in over a 10-fold increase in apparent peak efficiency, improving from approximately 10<sup>5</sup> plates/m to over a million plates/m. Simultaneously measured BSI and fluorescence electropherograms confirm that the efficiency increase is unique to the BSI signal and not due to changes in zone dispersion, and can be tuned to optimize separation resolution. The origin of the efficiency increase is discussed in terms of the refractive index and zone conductivity contributions to the BSI signal.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"13-21"},"PeriodicalIF":2.5,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145687171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board: Electrophoresis 23F25 编辑委员会：电泳23F25

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-12-02 DOI: 10.1002/elps.70063

引用次数: 0

Analysis of Colloidal Transport Mechanisms in Western Blotting Western Blotting中胶体运输机制分析。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-11-29 DOI: 10.1002/elps.70060

Carlos Vargas, Federico Méndez, Carlos Escobedo

{"title":"Analysis of Colloidal Transport Mechanisms in Western Blotting","authors":"Carlos Vargas, Federico Méndez, Carlos Escobedo","doi":"10.1002/elps.70060","DOIUrl":"10.1002/elps.70060","url":null,"abstract":"<div>\u0000 \u0000 <p>This work investigates electrokinetically driven protein transport in open microfluidic devices with microwells featuring axial shape variations. The results indicate that protein propagation, which is lysed at the surface, depends on two key parameters: the electrical potential ratio <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <mo>(</mo>\u0000 <mrow>\u0000 <mi>α</mi>\u0000 <mo>=</mo>\u0000 <mi>ζ</mi>\u0000 <mo>/</mo>\u0000 <msub>\u0000 <mi>ϕ</mi>\u0000 <mn>0</mn>\u0000 </msub>\u0000 </mrow>\u0000 <mo>)</mo>\u0000 </mrow>\u0000 <annotation>$( {alpha = zeta /{{phi }_0}} )$</annotation>\u0000 </semantics></math>, and the geometric curvature of the microwell. The concave microwell configuration presents the best outcome due to the emergence of a transverse velocity component that confines the cell within the microwell. Lastly, protein concentration can be improved when the negative microwell geometric slope exhibits nondifferentiable behavior (e.g., edges or fractal geometries), while a higher zeta potential can broaden the influence of the Stern layer.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"78-86"},"PeriodicalIF":2.5,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145631471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the Additional Pseudo-Crossover Frequency of Nanoparticles in Low Frequency Dielectrophoresis Regime 低频介质电泳中纳米颗粒附加伪交叉频率的表征。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-11-23 DOI: 10.1002/elps.70058

Tae Joon Kwak, Khaled S. Qananba, Mohammad Rizwen Ur Rahman, Chang-Koo Yun, Seungyeop Choi, Yong-Soo Choi, Sang Woo Lee, Woo-Jin Chang

{"title":"Characterization of the Additional Pseudo-Crossover Frequency of Nanoparticles in Low Frequency Dielectrophoresis Regime","authors":"Tae Joon Kwak, Khaled S. Qananba, Mohammad Rizwen Ur Rahman, Chang-Koo Yun, Seungyeop Choi, Yong-Soo Choi, Sang Woo Lee, Woo-Jin Chang","doi":"10.1002/elps.70058","DOIUrl":"10.1002/elps.70058","url":null,"abstract":"<div>\u0000 \u0000 <p>Dielectrophoresis (DEP) is a powerful tool for manipulating particles using non-uniform electric fields. This study combines numerical simulations and experiments to investigate crossover frequencies (COFs) for micro- and nanoparticles in a 3D microfluidic device with circular traps. MATLAB simulations revealed an inverse relationship between particle size and COF. For microparticles with diameters of 1.03, 2.27, 4.42, and 6.83 µm, the COFs were calculated as 769.10, 352.76, 183.96, and 120.51 kHz, respectively. For nanoparticles measuring 50, 170, and 500 nm, the corresponding COFs were 15.6, 4.62, and 1.57 MHz. These results closely matched experimental data. Notably, additional low-frequency pseudo-COFs emerged in experiments for nanoparticles ranging from 2 to 8 kHz (50 nm), 10 to 50 kHz (170 nm), and 40 to 100 kHz (500 nm). These frequencies proportionally increased with nanoparticle size and corresponded to unexpected negative DEP (nDEP)-like behavior under positive DEP (pDEP) conditions. This effect is attributed to low-frequency alternating current electroosmosis (ACEO), which dominates the DEP response of the nanoparticles smaller than 1 µm. These findings demonstrate strong agreement between numerical simulations and experimental results while also revealing the limitations of traditional models in predicting nanoparticle behavior under DEP. We expect that these results can also be applied to the manipulation of various bioparticles.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"137-147"},"PeriodicalIF":2.5,"publicationDate":"2025-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145586521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board: Electrophoresis 22F25 编辑委员会：电泳22F25

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-11-17 DOI: 10.1002/elps.70057

引用次数: 0

Microfluidic Dielectrophoretic Platform for the Manipulation of Brucella abortus Bacteria: Toward Rapid Diagnostic Solutions 用于流产布鲁氏菌操作的微流控介电泳平台：走向快速诊断解决方案。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-11-13 DOI: 10.1002/elps.70055

Katherine Acuña-Umaña, Estefany García-Martínez, Marco Mairena-Salazar, Nazareth Ruiz-Villalobos, Caterina Guzmán-Verri, Karina Torres-Castro, Leonardo Lesser-Rojas

{"title":"Microfluidic Dielectrophoretic Platform for the Manipulation of Brucella abortus Bacteria: Toward Rapid Diagnostic Solutions","authors":"Katherine Acuña-Umaña, Estefany García-Martínez, Marco Mairena-Salazar, Nazareth Ruiz-Villalobos, Caterina Guzmán-Verri, Karina Torres-Castro, Leonardo Lesser-Rojas","doi":"10.1002/elps.70055","DOIUrl":"10.1002/elps.70055","url":null,"abstract":"<div>\u0000 \u0000 <p>Brucellosis is a neglected zoonotic disease that continues to impact global public health and livestock economies, particularly in regions with limited diagnostic infrastructure. Its causative agent, <i>Brucella abortus</i>, is difficult to detect due to its intracellular lifestyle and the nonspecific symptoms it causes in humans. This study demonstrates the experimental application of dielectrophoresis (DEP) in a microfluidic device for the selective manipulation of polystyrene beads and inactivated <i>B. abortus</i> bacteria. By tuning the frequency and medium conductivity, reliable combined negative dielectrophoretic (nDEP) and hydrodynamic flow responses were achieved, leading to the deflection of bacterial cells across the microchannel within a critical vertical window for particle control. Distinct particle trajectories were observed under varying electric field conditions, confirming effective separation without the need for labels or biochemical markers, except for visual validation. This label-free strategy enables rapid sample processing and has the potential to be integrated into portable platforms for on-site diagnostics. The results highlight the feasibility of DEP-based approaches for pathogen separation and support their future implementation in brucellosis surveillance and point-of-care testing.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"68-77"},"PeriodicalIF":2.5,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145502810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board: Electrophoresis 21F25 编辑委员会：电泳21F25

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-11-07 DOI: 10.1002/elps.70056

引用次数: 0