ELECTROPHORESIS最新文献_第6页

Recent Developments in Capillary and Microchip Electroseparations of Peptides (2023–mid 2025) 毛细管和微芯片电分离多肽的最新进展（2023- 2025年中期）。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-11-06 DOI: 10.1002/elps.70052

Václav Kašička

{"title":"Recent Developments in Capillary and Microchip Electroseparations of Peptides (2023–mid 2025)","authors":"Václav Kašička","doi":"10.1002/elps.70052","DOIUrl":"10.1002/elps.70052","url":null,"abstract":"<p>This review presents a comprehensive overview of the developments and applications of high-performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical and biochemical characterization of peptides in the period from 2023 up to <i>ca</i>. the middle of 2025. Advances in the exploration of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, sorption suppression, EOF regulation, and detection, are described. New developments in the particular CE methods are presented, and several types of their applications are reported. They include qualitative and quantitative analysis of synthetic or isolated peptides, determination of peptides in complex biomatrices, peptide profiling of biofluids and tissue extracts, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They also deal with amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical and biochemical characterizations of peptides and their interactions with other (bio)molecules by the above CE methods are described.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"106-136"},"PeriodicalIF":2.5,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12827229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145458105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board: Electrophoresis 20F25 编辑委员会：电泳20F25

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-11-06 DOI: 10.1002/elps.70054

引用次数: 0

CRISPR-Cas9-Targeted Nanopore Sequencing for STR Typing 靶向crispr - cas9的纳米孔测序用于STR分型。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-11-05 DOI: 10.1002/elps.70051

Ting-Ting Yang, Jia-Rong Zhang, Zi-Han Xie, Zi-Lin Ren, Meng-Yang Zhao, Wen-Jing Hu, Jiang-Wei Yan, Ming Ni

{"title":"CRISPR-Cas9-Targeted Nanopore Sequencing for STR Typing","authors":"Ting-Ting Yang, Jia-Rong Zhang, Zi-Han Xie, Zi-Lin Ren, Meng-Yang Zhao, Wen-Jing Hu, Jiang-Wei Yan, Ming Ni","doi":"10.1002/elps.70051","DOIUrl":"10.1002/elps.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>CRISPR-Cas9-targeted sequencing can enrich DNA regions of interest by directing the Cas9 protein to bind and cleave specific DNA sequences via single-guide RNA (sgRNA). It is interesting to explore the efficacy of using CRISPR-Cas9-targeted nanopore sequencing (referred to as Cas9-seq), a polymerase chain reaction (PCR)-free workflow, for forensic short tandem repeats (STR) profiling, and to compare it with the amplification-based approach. In this pilot study, we constructed a Cas9-seq method for profiling seven STR loci, including D18S51, FGA, TPOX, D16S539, vWA, CSF1PO, and TH01. With 3 µg DNA inputs from human NA12878 and 293T cell lines, we achieved 643.45- and 468.34-fold enrichment ratios of the sgRNA-targeted regions by using Cas9-seq, respectively. Compared to nanopore sequencing of PCR amplicon products (amplicon-seq) of the ForenSeq DNA Signature Prep kit, the Cas9-seq reads had an ultralow strand bias. However, surprisingly, Cas9-seq did not show advantages in allele balance and had higher noise in the reads. At the seven STR loci for the two samples, both Cas9-seq and amplicon-seq had three genotyping errors. Additionally, there were no false-positive single-nucleotide polymorphisms (SNPs) introduced by Cas9-seq, whereas amplicon-seq produced three. In sum, we conclude that the PCR-free Cas9-seq might not be favorable for forensic STR genotyping.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"57-67"},"PeriodicalIF":2.5,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board: Electrophoresis 19F25 编辑委员会：电泳19F25

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-10-29 DOI: 10.1002/elps.70053

引用次数: 0

Polarization-Selective Dynamic Coupling: Electrorotation-Orbital Motion of Twin Colloids in Rotating Fields 偏振-选择动态耦合：旋转场中双胶体的电旋-轨道运动。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-10-25 DOI: 10.1002/elps.70050

Ye Tao, 陶冶, Rui Xue, 薛睿, Qisheng Wu, 武奇生, Binyu Wang, 王彬宇, Miao Fang, 方淼, Qingyu Ruan, 阮庆宇, Weiyu Liu, 刘维宇, Yukun Ren, 任玉坤

{"title":"Polarization-Selective Dynamic Coupling: Electrorotation-Orbital Motion of Twin Colloids in Rotating Fields","authors":"Ye Tao, 陶冶, Rui Xue, 薛睿, Qisheng Wu, 武奇生, Binyu Wang, 王彬宇, Miao Fang, 方淼, Qingyu Ruan, 阮庆宇, Weiyu Liu, 刘维宇, Yukun Ren, 任玉坤","doi":"10.1002/elps.70050","DOIUrl":"10.1002/elps.70050","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigates dynamic electrohydrodynamic (EHD) interactions between two identical colloidal microspheres in rotating electric fields using a fully coupled three-dimensional transient model. Long-range dielectrophoretic (DEP) attraction drives radial convergence; upon near-contact, tangential EHD sliding further induces asynchronous co-field orbital revolution. Crucially, individual electrorotation (ER) not only retains its original direction but also maintains a stable rate—with the rate deviating by <5% from that of isolated particles, matching single-particle behavior. High-frequency DEP force polarity reversal establishes stable noncontact equilibria via short-range repulsion. Spectral analyses reveal collective dynamics (radial mobility, orbital motion) stem from rotating electric field-mediated gap modulation rather than altered particle polarization. This dynamic decoupling—governed by the Kramers–Kronig relationship between real (radial DEP) and imaginary (rotational ER) components of polarizability—enables independent positional and rotational control, opening new avenues for noncontact colloidal manipulation in microfluidic mixers and dynamically reconfigurable active matter systems, where conventional DEP-based approaches are limited by coupled dynamics.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"43-56"},"PeriodicalIF":2.5,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145367782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-Read, High-Resolution Sanger Sequencing by Micelle-Tagging Electrophoresis 长读，高分辨率的Sanger测序胶束标记电泳。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-10-21 DOI: 10.1002/elps.70047

Randall Gamble, H. Michael Wenz, Bashar Mullah, James W. Schneider

{"title":"Long-Read, High-Resolution Sanger Sequencing by Micelle-Tagging Electrophoresis","authors":"Randall Gamble, H. Michael Wenz, Bashar Mullah, James W. Schneider","doi":"10.1002/elps.70047","DOIUrl":"10.1002/elps.70047","url":null,"abstract":"<p>We demonstrate a gel-free electrophoretic separation of Sanger sequencing fragments up to 782 bases in length using nonionic wormlike micelles as drag-tags in micelle-tagging electrophoresis (MTE). This is an increase of 280 bases over previous MTE methods and a nearly three-fold improvement over end-labeled free-solution electrophoresis (ELFSE) methods that use covalently attached drag-tags. For MTE, C18 alkane groups are attached to primers prior to their enzymatic extension. This alkane group provides a binding site for wormlike micelles composed of CiEj-type nonionic surfactants in the running buffer. Transient attachment of micelles to the C18 alkane group provides a highly uniform drag, equal to that of an ssDNA fragment 309 bases long. To account for slight mobility differences among the BigDye chain terminators, we developed a two-parameter time-shifting procedure to align the electropherograms for each termination chemistry. The increase in read length for this low-viscosity buffer (2.1 cP) is attributed to the alignment procedure, the large yet uniform drag, and the small degree of adsorption-based band broadening.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 1","pages":"5-12"},"PeriodicalIF":2.5,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12827225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board: Electrophoresis 18F25 编辑委员会：电泳18F25

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-10-17 DOI: 10.1002/elps.70049

引用次数: 0

Study on Dissociated States of Monocarboxylic Acids and Their Interactions With MCT1 by Capillary Electrophoresis With Interface-Induced Current Detector 毛细管电泳界面感应电流检测器研究单羧酸解离态及其与MCT1相互作用。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-10-17 DOI: 10.1002/elps.70028

Tao Huang, Chunsu Liang, Xiaomei Ling

{"title":"Study on Dissociated States of Monocarboxylic Acids and Their Interactions With MCT1 by Capillary Electrophoresis With Interface-Induced Current Detector","authors":"Tao Huang, Chunsu Liang, Xiaomei Ling","doi":"10.1002/elps.70028","DOIUrl":"10.1002/elps.70028","url":null,"abstract":"<div>\u0000 \u0000 <p>High-performance capillary electrophoresis (CE) has been widely applied in the analysis of organic acids, especially monocarboxylic acids (MAs), but no studies on the CE analysis of dissociated states of MAs have been reported. Here, 15 MAs were analyzed by the newly developed universal interface-induced current detector (IICRD). Two current signal peaks were observed in current electrophoretograms (CR-EGs), whereas only one peak with low sensitivity can be obtained by diode array detector (DAD). The current signal peaks of MAs were accurately identified by adding a charge-neutral marker and calculating by p<i>K</i><sub>a</sub>. The qualitative analysis indicated that the two current signal peaks of MAs were charge-neutral forms [MA<sup>±</sup>] and monovalent anions [MA<sup>−</sup>] in order. Quantitative analysis showed that CE-IICRD can enhance the sensitivity of MA analytes to 40 µM in limit of detection (LOD), with a linear range from 10<sup>−6</sup> to 10<sup>−2</sup> M. Furthermore, interactions between different dissociated states of MAs and monocarboxylate transporter 1 (MCT1) were studied by combined application of nonimmobilized cell CE (NICCE) method for the first time. The binding kinetic parameters and mole number of MCT1 on cell membranes can also be obtained. Besides, competitive binding experiments proved that BAY-8002 (MCT1-specific inhibitor) and lactic acid shared the same binding site. CE-IICRD provides a new method to reveal the interaction between different dissociated forms of MAs or other biomolecules and essential receptors.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 21","pages":"1576-1587"},"PeriodicalIF":2.5,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board: Electrophoresis 17F25 编辑委员会：电泳17F25

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-10-11 DOI: 10.1002/elps.70048

引用次数: 0

Study of Electrophoresis Process in Magnetic Colloids by Magnetic Methods 磁性胶体电泳过程的磁法研究。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-10-07 DOI: 10.1002/elps.70017

Yurii I. Dikansky, Andrey S. Drozdov, Dmitry S. Dorozhko

{"title":"Study of Electrophoresis Process in Magnetic Colloids by Magnetic Methods","authors":"Yurii I. Dikansky, Andrey S. Drozdov, Dmitry S. Dorozhko","doi":"10.1002/elps.70017","DOIUrl":"10.1002/elps.70017","url":null,"abstract":"<div>\u0000 \u0000 <p>Electrokinetic phenomena play a vital role in the study of colloidal nanoparticles, offering significant insights and applications across a wide range of fundamental research and practical uses. It is crucial to recognize the extensive research on various types of nanomaterials, including polymer latexes, quantum dots, and biomolecules. However, there is a significant gap in the study of magnetic systems. Such materials have immense potential and can greatly benefit from both magnetophoretic and electrophoretic techniques. In this work, the electrophoretic behavior of water-based magnetic fluids was investigated, focusing on how additional magnetic field exposure affects their properties. The studies conducted utilized magnetic measurements that emerged from the presence of colloidal particles within the examined systems, which exhibited both charge and magnetic moment. The magnetic susceptibility and magnetization of colloidal particles precipitate formed on one of the electrodes were measured. The thickness of the formed precipitate on the electrode can be confidently estimated through micrometric measurements as well as by analyzing its magnetic susceptibility during electrophoresis. A formula for calculating the electrophoretic velocity based on the results of magnetic measurements was obtained. Estimates of zeta potential and charge of colloidal particles were carried out. The electrophoresis process in these systems can be effectively regulated by an inhomogeneous magnetic field, leading to complete compensation.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 21","pages":"1569-1575"},"PeriodicalIF":2.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0