ELECTROPHORESIS最新文献

{"title":"A Consecutive Separation Strategy Using a 3D-Printed Microfluidic Chip to Achieve High-Purity White Blood Cells From Blood","authors":"Haoyuan Gu,&nbsp;Feng Yang,&nbsp;Chushan Gao,&nbsp;Zheda Zhang,&nbsp;Longlong Wang,&nbsp;Yubin Zhou,&nbsp;Wenlai Tang,&nbsp;Xiang Gao,&nbsp;Shu Zhu","doi":"10.1002/elps.70113","DOIUrl":"10.1002/elps.70113","url":null,"abstract":"<div>\u0000 \u0000 <p>Label-free and direct separation of white blood cells (WBCs) remains one of the major challenges in the field of efficient leukocyte separation. To address this challenge, we propose a WBC sorting strategy based on inertial microfluidics and 3D-printing technology, which employs a single inertial microfluidic chip to perform four consecutive high-throughput separation steps between red blood cells (RBCs) and WBCs. Specifically, the chip features a trapezoidal cross-section spiral channel to achieve size-dependent, stable spatial separation of cells along the inner and outer channels. After the conceptual design, the UV cured 3D printing technology is employed to fabricate the inertial chip and its fixture. Subsequently, mixed polystyrene microparticles are used as test objects to verify the sorting performance of the inertial chip, and the results indicate that the optimal separation flow rate is ∼1700 <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <mrow>\u0000 <mi>μ</mi>\u0000 <mi>L</mi>\u0000 </mrow>\u0000 <mo>/</mo>\u0000 <mi>min</mi>\u0000 </mrow>\u0000 <annotation>${mathrm{mu L}}/{mathrm{min}}$</annotation>\u0000 </semantics></math>. This corresponds to a maximum WBC throughput of ∼1.12 × 10⁷ cells/h during a single-pass separation under the current 1:100 dilution condition, assuming a whole-blood WBC concentration of 1.1 × 10⁷ cells/mL. Ultimately, the diluted whole-blood samples are used to examine the four-cycle consecutive sorting strategy, and the results demonstrated that this strategy, proposed in this work, could directly obtain WBCs from blood samples with a recovery efficiency of ∼94.9% and a purity of ∼85.5%. Related experiments indicate that this strategy which is centered on the 3D-printed chip offers high reliability and sensitivity for label-free WBC sorting from blood cells. With the aid of state-of-art additive manufacturing technology, it is promising that our strategy could be widely applied on fields including cell analysis and disease diagnosis.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"788-798"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148005047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclodextrins as Chiral Selectors in Capillary Electrophoresis—Recent Developments in Understanding Analyte Complexation and Applications in Pharmaceutical Analysis","authors":"Gerhard K. E. Scriba","doi":"10.1002/elps.70116","DOIUrl":"10.1002/elps.70116","url":null,"abstract":"<p>CDs, cyclic oligosaccharides consisting of α-1,4 linked <span>d</span>-glucopyranose units, have been applied in many areas, such as food, cosmetics, environmental, agriculture, textile, pharmaceutical, and chemical industries, due to their ability to form guest–host complexes. Because CDs are chiral compounds, they have also been successfully used as chiral selectors in analytical enantioseparation techniques, especially CE. In the last decades, multidisciplinary approaches have been performed, combining CE with NMR spectroscopy, molecular modeling, and other techniques in order to understand aspects of chiral recognition underlying enantioseparations. The present review focuses on such mechanistic studies published between January 2023 and March 2026 in addition to the application of CD-mediated CE enantioseparations to the determination of the enantiomeric purity of drug substances as well as the analysis of pharmaceutical formulations.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"696-712"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148143344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CMOS Electrokinetic Systems and Fabrication Approaches for On-CMOS 3D Electrodes","authors":"Lewis Keeble","doi":"10.1002/elps.70119","DOIUrl":"10.1002/elps.70119","url":null,"abstract":"<div>\u0000 \u0000 <p>Electrokinetics, including dielectrophoresis, electroosmosis and other phenomena, have become a popular technique for manipulating particles on the microscale using electric fields. Concurrently, CMOS microchips have become the dominant global technology. The combination of electrokinetics with CMOS appears ideal, as both utilise electric potentials, with the ability to make highly functional and scalable actuating systems. Since the first demonstration of electrokinetics implemented as part of a CMOS system in 2003, 49 further studies were published. But whilst the first study led to a commercialised product, those that followed have not achieved the same success. This review surveys the available literature on CMOS-based electrokinetic systems and highlight key trends in design. The obstacles blocking the field from greater success are explored and opportunities are identified, with future perspectives provided throughout. The review shows that, currently, the foremost limitation for CMOS-based electrokinetic systems lies with the front-end—namely, the electrode systems being employed. The need to extend beyond the planar configurations currently being used and fabricate 3D electrodes on-CMOS that can operate within high conductivity environments is put forward. This discussion then leads into a review on fabrication approaches for 3D electrodes with a specific focus on those compatible with a CMOS system.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"754-778"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148263850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board: Electrophoresis 8'26","authors":"","doi":"10.1002/elps.70141","DOIUrl":"https://doi.org/10.1002/elps.70141","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"685-688"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrophoresis of Charged Dielectric Droplet With Ion Concentration Polarization Effect","authors":"Yunfan Huang,&nbsp;Moran Wang","doi":"10.1002/elps.70118","DOIUrl":"10.1002/elps.70118","url":null,"abstract":"<div>\u0000 \u0000 <p>Droplet electrophoresis is one of the most commonly used methods for measuring the charge at two-liquid interfaces. However, there is still a lack of analytical solutions that consider the ion concentration polarization mechanism with asymmetric ion distribution under high surface charge. Based on the thin electric double layer assumption, we construct the asymptotic form of the macroscopic model of dielectric droplet electrophoresis and establish the coarse-grained effective interface condition across the diffuse layer regions through the heuristic treatment extending the method of matched asymptotic expansion, revealing the coupling mechanism between ion concentration polarization and permittivity-induced inhomogeneous charging. An analytical solution under the weak-external-field limit is provided, demonstrating a nonmonotonic dependence of electrophoretic mobility on the <span></span><math>\u0000 <semantics>\u0000 <mi>ζ</mi>\u0000 <annotation>$zeta$</annotation>\u0000 </semantics></math> potential under weak external electric fields. By integrating previous experimental results on droplet electrophoresis, a concise and physically meaningful quantitative model of the charging mechanism for general nonpolar oil is established.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 8","pages":"724-743"},"PeriodicalIF":2.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probing Chiral Recognition on Amylose Tris(3,5-Dimethylphenylcarbamate) Using Cinnamyl 2-Aminoanilides: The Subtle Impact of Aliphatic Substituents","authors":"Selen Gözde Kaya,&nbsp;Alessia Raucci,&nbsp;Clemens Zwergel,&nbsp;Antonello Mai,&nbsp;Sergio Valente,&nbsp;Roberto Cirilli","doi":"10.1002/elps.70109","DOIUrl":"10.1002/elps.70109","url":null,"abstract":"<p>This study investigates the chiral recognition behavior of the polysaccharide‑based chiral stationary phase amylose tris(3,5‑dimethylphenylcarbamate) (Chiralpak AD‑3) under polar organic conditions, using a series of racemic cinnamyl 2‑aminoanilides differing only in the nature of their aliphatic substituents. Remarkably large differences in enantioselectivity were observed among closely related compounds, with enantioseparation factors reaching exceptionally high values (α up to 37) when neat 2‑propanol was employed as the mobile phase. In contrast, replacement of 2‑propanol with 1‑propanol led to a dramatic reduction or complete loss of chiral discrimination, highlighting the crucial role of solvent structure in modulating analyte–selector interactions. Analysis of retention and selectivity trends suggests that chiral recognition primarily arises from hydrogen bonding involving the anilide NH₂ group, combined with other interactions modulated by the aliphatic side chains. These findings emphasize how subtle structural modifications, together with mobile phase composition, can profoundly affect enantioselectivity on polysaccharide‑based chiral stationary phases.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 7","pages":"613-619"},"PeriodicalIF":2.6,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147971375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paper-Based Electrochemical Aptasensor Chip for Ultrasensitive Glyphosate Detection Enabled by MXene–Au and Au@NiCo LDH Nanozyme Amplification","authors":"Yaqi Yang,&nbsp;Hongxiao Sun,&nbsp;Ke Sun,&nbsp;Longwen Fu,&nbsp;Na Zhou,&nbsp;Wenhai Wang,&nbsp;Lingxin Chen,&nbsp;Bowei Li","doi":"10.1002/elps.70106","DOIUrl":"10.1002/elps.70106","url":null,"abstract":"<div>\u0000 \u0000 <p>As one of the world's most widely used herbicides, glyphosate (Gly) poses considerable ecological and human health risks due to its environmental persistence and capacity to bioaccumulate through food webs in both terrestrial and aquatic ecosystems. Thus, developing analytical methods for Gly is critical for environmental monitoring, food safety, and public health protection. In this study, we combine electrochemical detection with paper-based microfluidics to develop a novel rotary paper-based aptasensor for highly selective and sensitive Gly quantification in environmental samples. Thiolated capture DNA (cDNA) is immobilized on the electrode surface via stable Au–S bonds, forming a well-defined recognition interface. An apt-Au@NiCo LDH with intrinsic peroxidase-like activity is then anchored via complementary base pairing. Gly binding to its specific aptamer triggers measurable electrochemical signal changes, enabling precise recognition and quantification. The sensor achieves a wide linear range of 0.1 pg/mL–10 ng/mL, and the limit of detection (LOD) is 0.05 pg/mL. This work provides a novel, efficient, economical, and practical solution for on-site, rapid, and ultrasensitive Gly detection in environmental samples. The platform shows strong promise for real-world applications.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 7","pages":"666-674"},"PeriodicalIF":2.6,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147940735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board: Electrophoresis 7'26","authors":"","doi":"10.1002/elps.70129","DOIUrl":"https://doi.org/10.1002/elps.70129","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 7","pages":"581-584"},"PeriodicalIF":2.6,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148533578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enantioseparation of Chiral Compounds From Traditional Chinese Medicines on a Chiral Packed Capillary Column With UV Detection","authors":"Shiyun Hu,&nbsp;Xiao Hu,&nbsp;Kun Chen,&nbsp;Yingying Liu,&nbsp;Yingying Deng,&nbsp;Lishuang Yu,&nbsp;Lu Huang","doi":"10.1002/elps.70105","DOIUrl":"10.1002/elps.70105","url":null,"abstract":"<div>\u0000 \u0000 <p>A chiral packed capillary column (PCC) was prepared by a photonic crystal fiber (PCF) as the frit and cellulose tris(3,5-dimethylphenylcarbamate)-coated silica particles (CDMPC@SiO₂) as the chiral stationary phase. This column coupled with UV detection was employed for the enantioseparation of chiral compounds derived from traditional Chinese medicines (TCMs). Baseline enantioseparation was achieved for schisandrin B and tetrahydropalmatine, whereas partial separation was observed for anisodamine and corynoxeine. The reproducibility and stability of the column were confirmed, with relative standard deviation (RSD) values for retention time and resolution ranging from 2.5% to 3.2% (intra-day), 2.7% to 3.5% (inter-day), and 4.1% to 4.5% (inter-column). Then, the column was successfully applied to the direct enantioseparation and quantitative determination of tetrahydropalmatine in the extract of Yanhusuo (<i>Corydalis Rhizoma</i>). These results demonstrate that the proposed chiral CDMPC@SiO₂ PCC is not only reproducible and stable but also can be effectively applied to the enantioseparation and content determination of chiral compounds in complex TCMs. Furthermore, density functional theory (DFT) calculations were conducted to investigate intermolecular interactions between CDMPC and chiral analytes, which provides a deeper understanding of the enantioseparation mechanism.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 7","pages":"585-594"},"PeriodicalIF":2.6,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Pentaplex Reverse Transcription Droplet Digital PCR Assay for Simultaneous Detection and Absolute Quantification of HIV-1, HIV-2, HCV, and HBV With Internal Control","authors":"Soo Yeon Lim,&nbsp;Un Na Koh,&nbsp;Ah Leum Kim,&nbsp;Yebin Kim,&nbsp;Ga Eun Kim,&nbsp;Si-Keun Lim","doi":"10.1002/elps.70098","DOIUrl":"10.1002/elps.70098","url":null,"abstract":"<p>This study presents the development of a pentaplex reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay for the simultaneous detection and absolute quantification of human immunodeficiency virus (HIV)-1, HIV-2, hepatitis C virus (HCV), hepatitis B virus (HBV), and an internal control. These viruses are important blood-borne pathogens for diagnostics, blood screening, and the validation of reference materials. Commercial kits used for blood screening allow multiplex detection but rely on relative, cycle threshold-based quantification approaches; consequently, the results may vary depending on reference materials and analytical conditions, which limit interlaboratory comparability. In addition, conventional antigen–antibody assays and single-target quantitative polymerase chain reaction (qPCR) methods have limited multiplexing capability and require standard curves, restricting their broader application. The assay demonstrated 95% limit of detection (LoD) values ranging from 2.42 to 6.65 copies/reaction across targets with high specificity. Validation following the principles outlined in International Organization for Standardization (ISO) 20395:2019 demonstrated the linearity, sensitivity, and precision of the assay, with low coefficients of variation indicating high reproducibility. When evaluated using a nanowell plate-based system, the assay showed comparable performance without statistically significant differences, thereby demonstrating platform scalability. The applicability of the assay was further confirmed using externally sourced pathogen resources, including human plasma–derived HIV-1–positive materials under conditions resembling clinical specimens, demonstrating reliable detection beyond international reference materials. This study introduces a robust multiplex platform for diagnostics, blood screening, and quality control of reference materials. The assay integrates qualitative and quantitative analysis within a single workflow, enabling efficient multi-target detection.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 7","pages":"629-643"},"PeriodicalIF":2.6,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148028430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}