ELECTROPHORESIS最新文献

{"title":"Streaming-Particle Method for Dielectrophoretic Characterization.","authors":"A K M Fazlul Karim Rasel, Eron P Ristich, Mark A Hayes, Sean L Seyler","doi":"10.1002/elps.8146","DOIUrl":"https://doi.org/10.1002/elps.8146","url":null,"abstract":"<p><p>Fully characterizing subtle differences in biologically important particles-including peptides, proteins, protein complexes, exosomes, viruses, organelles, and cells-is essential, as any alteration can impact their function. Detailed bioparticle characterization has broad implications for biomedical engineering, health care, food science, astrobiology, environmental studies, and microbiology. Dielectrophoresis (DEP) generates distinct forces based on subtle structural differences between bioparticles and has the potential to enable full characterization by quantifying the DEP response of a particle. However, current DEP techniques primarily rely on particle trapping, which presents limitations, particularly for nanoparticles. In contrast, streaming-based DEP measurement techniques remain largely unexplored. Here, we introduce a streaming-based microfluidic method inspired by the (inverse) classical scattering problem in physics. Using a custom insulator-based DEP microchannel (iDEP), the DEP susceptibility of a particle is quantified based on its predictable deflection magnitude. We demonstrate the feasibility of this approach for negative DEP using finite element analysis to conduct numerical scattering experiments on representative nanoparticles. To fully capture diffusion effects, we solved the steady-state Smoluchowski advection-diffusion equation to obtain concentration fields in the microchannel and extract realistic scattering profiles. Additionally, deterministic particle trajectories, computed in the absence of diffusion, were analyzed using streamline analysis to support the advection-diffusion results. Our results indicate that, under optimal conditions, the prototype iDEP microchannel approaches the necessary sensitivity for protein DEP characterization, even when diffusion is included. Like existing iDEP devices, a real iDEP scattering instrument is expected to be easy and inexpensive to operate. Combined with the straightforward processing and interpretation of the scattering data, the iDEP scattering technique has the potential to enable high-throughput, accurate bioparticle characterization.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From One Strand Dyed/Undyed Hair With/Without Root to Fast and Successful STR Profiling and Evaluation With Principle Component Analysis.","authors":"Gulten Rayimoglu, Fatma Cavus Yonar, Beril Anılanmert","doi":"10.1002/elps.8142","DOIUrl":"https://doi.org/10.1002/elps.8142","url":null,"abstract":"<p><p>Fallen/shed single hair shafts with/without roots can be encountered as evidence in crime scenes. Identification from hair exhibits serious difficulties, due to the low amount of DNA, PCR inhibitors, and the existence of DNA mostly in degraded forms. Exposure of hair to chemical oxidation, sunlight, etc. brings extra difficulties to DNA analysis. In single hair shafts, no satisfactory nuDNA test method that can be used both for dyed/undyed single hair shafts with/without root exists in the literature. Next-generation systems are expensive, have a labor-intensive workflow, and are not available in many forensic laboratories. Here an STR profiling method has been developed modifying an isolation kit method for an easy, successful DNA for (1) dyed/undyed single hairs with root and (2) without root from 36 individuals and compared with the unmodified method (total 432 analyzed samples). Modified silica-based isolation method, yielded 0.65-1.05 ng/µL DNA. The success of STR typing after capillary electrophoresis was 96%-98% for dyed/undyed hair with roots and 89%-93% for rootless ones, while the results for the unmodified method were 67%-82%. The results were investigated using PCA, t-test, and F-test. This protocol is simple, inexpensive, does not require automation, and can be performed using kits/equipment available in every forensic laboratory for forensic genotyping.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the MC1R Gene Sequence Variation Using Oxford Nanopore Sequencing","authors":"Wojciech Branicki,&nbsp;Aleksandra Pisarek-Pacek,&nbsp;Kamila Marszałek,&nbsp;Agata Jarosz,&nbsp;Magdalena Kukla-Bartoszek,&nbsp;Magdalena Zubańska,&nbsp;Agnieszka Bronikowska,&nbsp;Katarzyna Węgrzyn,&nbsp;Bożena Wysocka,&nbsp;Magdalena Spólnicka,&nbsp;Ewelina Pośpiech","doi":"10.1002/elps.8141","DOIUrl":"10.1002/elps.8141","url":null,"abstract":"<div>\u0000 \u0000 <p>The <i>MC1R</i> gene, which is responsible for most cases of red hair, affects other hair and skin colours and contributes to differences in pain sensitivity and consists of a single exon with a very high level of allelic heterogeneity. In this research, we show that the Oxford Nanopore Technology (ONT) offers a good alternative to study the <i>MC1R</i> sequence variation. MinION was used to sequence the 1590 bp <i>MC1R</i> exon and minimal promoter in a cohort of 126 subjects, including 65 red-haired individuals, using the FLO-MIN106 (R9.4) chemistry. Assigned DNA variants were validated using Ion Torrent technology provided with Ion Xpress Plus Fragment Library Kit and the Personal Genome Machine^TM (PGM^TM). We show that the use of the latest sequencing kit V14 together with the FLO-MIN114 (R10.4.1) flow cell has eliminated the systematic errors observed with the previous chemistry and allowed reliable detection of short indels important for phenotypic inference. Importantly, the use of the algorithm implemented in the EPI2ME software enabled convenient and accurate read-based phase determination which can be useful in data interpretation.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 11-12","pages":"727-731"},"PeriodicalIF":2.5,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Size-Dependent Acoustophoresis in Rectangular Microchannels: Critical Particle Radius and Multidimensional Field Coupling Effects.","authors":"Junjun Lei","doi":"10.1002/elps.8151","DOIUrl":"https://doi.org/10.1002/elps.8151","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Particle manipulation using acoustophoresis has emerged as a pivotal technology in microsystems engineering, garnering significant attention across soft matter physics, biomedical diagnostics, and lab-on-a-chip applications. The operational efficacy of acoustofluidic tweezers platforms hinges on the precise control of the critical particle size threshold that governs the transition between radiation-force-dominated trapping and streaming-mediated transport. Through systematic numerical investigations in rectangular microchannels with two-dimensional (2D) confinement, this study establishes quantitative correlations between critical radius thresholds and geometric parameters in coupled standing-wave fields. The critical radius &lt;math&gt; &lt;semantics&gt;&lt;msubsup&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt; &lt;mi&gt;c&lt;/mi&gt;&lt;/msubsup&gt; &lt;annotation&gt;$r_p^c$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; demarcates two distinct transport regimes: (i) Supercritical particles ( &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;&gt;&lt;/mo&gt; &lt;msubsup&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt; &lt;mi&gt;c&lt;/mi&gt;&lt;/msubsup&gt; &lt;/mrow&gt; &lt;annotation&gt;${r}_p &gt; r_p^c$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) achieve stable nodal trapping via dominant radiation forces, independent of initial spatial distribution; (ii) subcritical particles ( &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;&lt;&lt;/mo&gt; &lt;msubsup&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt; &lt;mi&gt;c&lt;/mi&gt;&lt;/msubsup&gt; &lt;/mrow&gt; &lt;annotation&gt;${r}_p &lt; r_p^c$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) undergo continuous advection through streaming vortices. Our multiphysics framework combines finite element modeling with analytical validation through boundary-layer force equilibrium analysis, revealing three key findings: First, geometric confinement induces characteristic scaling laws ( &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msubsup&gt;&lt;mi&gt;r&lt;/mi&gt; &lt;mi&gt;p&lt;/mi&gt; &lt;mi&gt;c&lt;/mi&gt;&lt;/msubsup&gt; &lt;mo&gt;∝&lt;/mo&gt; &lt;msqrt&gt;&lt;mi&gt;l&lt;/mi&gt;&lt;/msqrt&gt; &lt;/mrow&gt; &lt;annotation&gt;$r_p^c propto sqrt l $&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; with &lt;math&gt;&lt;semantics&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;annotation&gt;$l$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; being the channel size) for one-dimensional (1D) standing waves, confirmed through parametric studies ( &lt;math&gt;&lt;semantics&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;annotation&gt;$l$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; : 0.1-5.1 mm). Second, 1D models maintain predictive accuracy (with discrepancies &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;γ&lt;/mi&gt; &lt;mo&gt;&lt;&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt; &lt;mo&gt;%&lt;/mo&gt;&lt;/mrow&gt; &lt;annotation&gt;$gamma &lt; 5% $&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) for systems with wavelength ratios &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mi&gt;y&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mi&gt;x&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;&gt;&lt;/mo&gt; &lt;mn&gt;4&lt;/mn&gt;&lt;/mrow&gt; &lt;annotation&gt;${lambda }_y/{lambda }_x &gt; 4$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; or &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mo&gt;&lt;&lt;/mo&gt; &lt;mn&gt;0.25&lt;/mn&gt;&lt;/mrow&gt; &lt;annotation&gt;$ &lt; 0.25$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , but fail in coupled-mode fields ( &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mn&gt;0.25&lt;/mn&gt; &lt;mspace&gt;&lt;/mspace&gt; &lt;mfrac&gt;&lt;mo&gt;&lt;&lt;/mo&gt; &lt;mo&gt;∼&lt;/mo&gt;&lt;/mfrac&gt; &lt;mspace&gt;&lt;/mspace&gt; &lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mi&gt;y&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mi&gt;x&lt;/","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Electrophoretic Mobility Determination in Nanoparticle Analysis: Two Parallel Techniques Converging in a Distinctive Parameter","authors":"Carlos Adelantado,&nbsp;Jan Jordens,&nbsp;Stefan Voorspoels,&nbsp;Milica Velimirovic,&nbsp;Kristof Tirez","doi":"10.1002/elps.202400132","DOIUrl":"10.1002/elps.202400132","url":null,"abstract":"<p>A critical comparison of the main parameters playing a role in measurement of electrophoretic mobility of plastic nanoparticles (NPs) by CE and laser Doppler velocimetry (LDV) techniques in NP suspensions is herein presented, accompanied by a discussion about potential impact on different mobility values observed. Capillary material and dynamic or permanent coating of the inner capillary wall, capillary dimensions, EOF variability, BGE temperature, Joule heating, and presence of species potentially interacting with analyzed NPs are underlined as possible causes of the different performance of the above two techniques. It is of importance to get an insight into the reasons behind experimental conditions and operating features to opt for one technique or the other based on research interests. In the end, it is intended to present a knowledge expansion about two parallel paths that converge in a distinctive parameter of an enormous relevance in CE, effective electrophoretic mobility, not achievable by other techniques, and discuss practical considerations in experimental design.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 11-12","pages":"743-751"},"PeriodicalIF":2.5,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contents: Electrophoresis 7–8'25","authors":"","doi":"10.1002/elps.202570042","DOIUrl":"10.1002/elps.202570042","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 7-8","pages":"355-356"},"PeriodicalIF":2.5,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board: Electrophoresis 7–8'25","authors":"","doi":"10.1002/elps.202570041","DOIUrl":"10.1002/elps.202570041","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 7-8","pages":"353-354"},"PeriodicalIF":2.5,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202570041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coupling Capillary Electrophoresis With a Shifted Inlet Potential High-Resolution Ion Mobility Spectrometer","authors":"Klaus Welters,&nbsp;Christian Thoben,&nbsp;Christian-Robert Raddatz,&nbsp;Florian Schlottmann,&nbsp;Stefan Zimmermann,&nbsp;Detlev Belder","doi":"10.1002/elps.8147","DOIUrl":"10.1002/elps.8147","url":null,"abstract":"<p>We present the coupling of capillary electrophoresis to a custom-built high-resolution ion mobility spectrometer (IMS). This system integrates a shifted inlet potential IMS configuration with a customised nanoflow ESI sheath interface. It enables the rapid analysis of quaternary ammonium compounds (QACs) and their impurities in real-world samples. It allowed the detection of six non-chromophoric compounds in about 3 min. The assignment of the IMS signals to compounds was supported by matching experimentally determined collision cross-section (CCS) values with predicted values. The system achieved a detection limit in the single-digit picogram range with IMS resolutions of over 80.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 11-12","pages":"694-701"},"PeriodicalIF":2.5,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.8147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gradient Insulator-Based Dielectrophoresis of Gold Nanoparticles","authors":"Alex J. Ramirez,&nbsp;A. K. M. Fazlul Karim Rasel,&nbsp;Sean L. Seyler,&nbsp;Mark A. Hayes","doi":"10.1002/elps.8119","DOIUrl":"10.1002/elps.8119","url":null,"abstract":"<div>\u0000 \u0000 <p>Various forms of dielectrophoresis and higher order electrokinetic effects are being increasingly investigated and used to precisely and accurately manipulate micro and nanoparticles within microfluidic devices. The types of particles span ∼10 nm to hundreds of microns in diameter and are composed of minerals, polymers, biological materials, and complex mixtures. Some studies focused on the selective isolation and concentration of purified particles countering negative dielectrophoretic forces against flow and electrophoretic effects. Similar studies are presented here examining the behaviors of small inorganic particles (10 nm diameter) where their collective actions are inconsistent with negative dielectrophoretic effects and were consistent overall with positive dielectrophoresis (DEP). Positive DEP can account for some of the observed phenomena, particularly the deflection of large particle aggregates, which are rapidly accelerated through microchannel constrictions and then pulled back toward the constrictions against the direction of electroosmotic flow. Nevertheless, the dynamic complexity of the observed nanoparticle structures suggests that a myriad of electrostatic and possibly hydrodynamic forces, including both particle–particle and particle–device interactions, may be involved.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 11-12","pages":"768-776"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Controllable Cargo Delivery Vehicle Driven by Electrically Actuated Galinstan Droplets","authors":"Qingming Hu,&nbsp;Fengshi Hu,&nbsp;Dandan Sun,&nbsp;Kailiang Zhang","doi":"10.1002/elps.8143","DOIUrl":"10.1002/elps.8143","url":null,"abstract":"<div>\u0000 \u0000 <p>As an emerging functional material, liquid metal has attracted extensive attention due to its unique physical/chemical properties. Particularly, the combination of the intrinsic fluidity with rapidly stimuli-responsiveness to electrical field endows it potential as soft actuators to be employed in soft robots. Herein, we developed a small controllable vehicle driven by electrically actuated Galinstan droplets. A series of experiments were carried out to evaluate the vehicle's performance, including straight translational locomotion at various speeds and rotational motion from different starting angles. And then, the vehicle's excellent mobility is further demonstrated through its ability to follow complex trajectories. More importantly, by redesigning the vehicle's frame, it can be adapted for multiple functions, such as cargo transportation and loading/unloading tasks. The present finding is envisaged to have the potential to expand current research on soft robot and further advance the development of micro-factory.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 11-12","pages":"777-786"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}