ELECTROPHORESIS最新文献

{"title":"Back Cover: Analytical Methods to Evaluate RNA Circularization Efficiency","authors":"Yali Sun,&nbsp;Anis H. Khimani,&nbsp;Yanhong Tong,&nbsp;Zhi-xiang Lu","doi":"10.1002/elps.202470115","DOIUrl":"https://doi.org/10.1002/elps.202470115","url":null,"abstract":"<p>DOI: 10.1002/elps.202400067</p><p>The cover image is based on the Article <i>Analytical Methods to Evaluate RNA Circularization Efficiency</i> by Yali Sun et al., https://doi.org/10.1002/elps.202400067. Created with BioRender.com.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 21-22","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202470115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142868792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inferring Distant Relationships From Dense SNP Data Utilizing Two Genealogy Algorithms.","authors":"Xinyi Lin, Shuang Han, Nan Zhang, Xiaohua Ling, Zhaochen Bai, Xueling Ou","doi":"10.1002/elps.202400208","DOIUrl":"https://doi.org/10.1002/elps.202400208","url":null,"abstract":"<p><p>A highly esteemed method known as investigative genetic genealogy (IGG) has been developed to identify DNA samples from forensic crime scenes and human remains of disaster victims. With the advent of next-generation sequencing, it is now feasible to access information on millions of SNPs typed in a single sequencing run that fulfill the requirements for kinship inference. However, challenges such as the poor quality of forensic samples, the high cost associated with sequencing technology, and privacy concerns regarding large-scale genetic databases remain unresolved in this field. In the present study, we validated the identification of relationships up to the seventh degree using two genealogy algorithms (IBIS and KING) under various parameter settings. This was accomplished through whole genome sequencing data derived from two southern Chinese Han pedigrees during an initial phase, while also exploring workflows adapted for low-quality samples. To achieve this objective, low-coverage whole genome sequencing data were downsampled from high-coverage original datasets; additionally, mimic SNP array data-containing less information but offering greater accessibility-were prepared as reference samples. Through a series of experimental analyses, we not only validate the applicability of selected processing procedures and inference tools for low-coverage samples but also proposed that a meticulously crafted site filtering strategy can significantly improve the accuracy of kinship identification. This acknowledges the necessity for further systematic evidence in future research endeavors.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring Addiction to Pregabalin in Northern Algeria by Using Hair Testing","authors":"Tehami Soumia,&nbsp;Bertaso Anna,&nbsp;Murari Matilde,&nbsp;Djafer Rachid,&nbsp;Federica Bortolotti,&nbsp;Gottardo Rossella","doi":"10.1002/elps.202400161","DOIUrl":"10.1002/elps.202400161","url":null,"abstract":"<p>Pregabalin (PGB) is a novel gamma-aminobutyric acid analog that has been recently approved for the treatment of partial-onset seizures, neuropathic pain, and fibromyalgia. Although PGB presents a low potential for abuse in comparison to other scheduled drugs, the literature reports its potential misuse, especially among individuals with former or current drug addiction. The present study aimed to develop and validate a novel method for the determination of PGB in hair to perform a retrospective observational study on the misuse of this drug in a population of addicted subjects from Northern Algeria. Hair and urine samples were collected from patients (<i>n</i> = 79) with a history of illicit drug use, monitored by the Intermediate Addiction Care Center of Constantine (Algeria). None of the patients had a medical prescription for PGB. Samples had also been tested for the presence of other drugs of abuse with routine LC–MS/MS methods. Hair samples (∼25 mg) were analyzed for PGB after sonication for 2 h in acetonitrile. The organic phase was dried and reconstituted in 100 µL mobile phase and analyzed by LC–MS/MS. The method was fully validated with LOD and LOQ results of 10 and 20 pg/mg, respectively. The linearity was verified in the 20–1000 pg/mg range. PGB was found in the hair samples of 51/79 subjects, with concentrations ranging from traces to 380 pg/mg. The study revealed that about 65% of the subjects used PGB in the absence of any medical prescription, indicating the potential misuse of this drug.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 5-6","pages":"316-321"},"PeriodicalIF":3.0,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Error Suppression for Accurate Detection of Low-Frequency Variants","authors":"Huimin Chen,&nbsp;Fei Yu,&nbsp;Debin Lu,&nbsp;Shiyue Huang,&nbsp;Songrui Liu,&nbsp;Boseng Zhang,&nbsp;Kunxian Shu,&nbsp;Dan Pu","doi":"10.1002/elps.202400202","DOIUrl":"10.1002/elps.202400202","url":null,"abstract":"<div>\u0000 \u0000 <p>The identification of low-frequency variants remains challenging due to the inevitable high error rates of next-generation sequencing (NGS). Numerous promising strategies employ unique molecular identifiers (UMIs) for error suppression. However, their efficiency depends highly on redundant sequencing and quality control, leading to tremendous read waste and cost inefficiency. Here, we describe a novel approach, enhanced error suppression strategy (EES), that addresses these challenges by (1) optimizing data utilization and reducing read waste by utilizing single-read correction that reserves abundant single reads that complement other single reads or single-strand consensus sequences (SSCSs), and (2) effectively enhancing the accuracy of NGS by employing Bayes’ theorem. EES significantly improves variant detection accuracy, achieving a background error rate of less than 4.4 × 10⁻⁵ per base pair. Additionally, the data utilization rate is dramatically increased, with a 22.9-fold enhancement in duplex consensus sequence (DCS) recovery compared to traditional methodologies. Furthermore, EES demonstrates superior error suppression performance across various base substitutions. In conclusion, EES represents a significant advancement in detecting low-frequency variants by improving data utilization and reducing sequencing errors. It potentially enhances the sensitivity and accuracy of NGS applications, proving highly valuable in clinical and research contexts where precise variant detection is critical.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 1-2","pages":"65-75"},"PeriodicalIF":3.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fundamentals of Viscoelastic Particle Focusing in Suddenly Expanded Microfluidic Channels","authors":"Dalin Chen,&nbsp;Di Huang,&nbsp;Zhixian Zhu,&nbsp;Qiang Huang,&nbsp;Zhonghua Ni,&nbsp;Yiduo Wang,&nbsp;Nan Xiang","doi":"10.1002/elps.202400225","DOIUrl":"10.1002/elps.202400225","url":null,"abstract":"<div>\u0000 \u0000 <p>Particles focusing in suddenly expanded microfluidic channels with viscoelastic biofluids have attracted increased interests due to the potential biomedical applications. In this work, three types of suddenly expanded channels were designed and employed for experiments. These suddenly expanded channels contained a certain number of triangular expansion structures on the sides of the straight channel to investigate the effect of secondary flow on particle viscoelastic focusing and we investigated particle focusing behavior in those three channels with 0.1% hyaluronic acid (HA) solution. The effects of various suddenly expanded channel structures on particle focusing were discussed. The results indicated that the viscoelastic focusing effect was enhanced by using the sudden expansion structures. Consequently, particles with multi-peak distributions were successfully focused into a focusing stream by using the secondary flow induced by the suddenly expanded structure. Finally, the viscoelastic focusing of leukocytes was explored using suddenly expanded channels. This work offers a deep understanding on the fundamentals of particle focusing in suddenly expanded channels and the results will enable the design of particles focusing microfluidic devices with suddenly expanded channels for critical applications in biomedicine.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 3-4","pages":"183-191"},"PeriodicalIF":3.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrokinetic Power-Series Solution in Narrow Cylindrical Capillaries for All Zeta Potentials.","authors":"Sam Khalifa, Arturo Villegas, Francisco J Diez","doi":"10.1002/elps.202400183","DOIUrl":"https://doi.org/10.1002/elps.202400183","url":null,"abstract":"<p><p>Work from Rice and Whitehead showed the results of electrokinetic flow in a capillary tube under the assumption of low zeta potential <math><semantics><mo><</mo> <annotation>$<$</annotation></semantics> </math> 25 mV, limiting the approximation's usability. Further research conducted by Philip and Wooding provided an alternative solution that assumes high zeta potentials <math><semantics><mo>></mo> <annotation>$>$</annotation></semantics> </math> 25 mV and relies on Rice and Whitehead's solution for lower ranges. However, this solution is presented as a piecewise function, where the functions change based on the zeta potential and the <math> <semantics><mrow><mi>κ</mi> <mi>a</mi></mrow> <annotation>$kappa a$</annotation></semantics> </math> parameter, introducing infinite values for the zeta potential and discontinuities in the derived functions. This paper aims to provide a singular equation solution to the full Poisson-Boltzmann equation for a long cylindrical capillary for all zeta potentials. This solution is a single, continuous, and finite function that produces exact results instead of approximations for all ranges of zeta potential. This exact solution is compared against published approximate solutions for large zeta potentials shown by comparing the large zeta potential approximation with the new exact solution. Important parameters such as volume transport and apparent viscosity were found to have errors of up to 9.76%-57.4%, respectively. The function <math> <semantics><mrow><mi>f</mi> <mo>(</mo> <mi>κ</mi> <mi>a</mi> <mo>,</mo> <msub><mi>ψ</mi> <mn>0</mn></msub> <mo>,</mo> <mi>β</mi> <mo>)</mo></mrow> <annotation>$f(kappa a, psi _0, beta)$</annotation></semantics> </math> has errors of up to 10.5% compared to our full solution.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-Step Focusing of Ampholytes With Electrophoretic Mobilization: Concepts Assessed by Computer Simulation.","authors":"Wolfgang Thormann, Richard A Mosher","doi":"10.1002/elps.202400215","DOIUrl":"https://doi.org/10.1002/elps.202400215","url":null,"abstract":"<p><p>The dynamics of three one-step focusing protocols described in the literature for IEF-MS analyses of proteins are assessed by computer simulation. Focusing of 101 carrier ampholytes (pI range 3.0-11.0) and 9 analytes (pI range 3.9-10.4) with concurrent electrophoretic mobilization in an electroosmosis-free environment is studied via use of the following: (1) an acid as anolyte and catholyte, (2) a plug of a base between the sample and the acid as catholyte, and (3) a base incorporated into the acid-based catholyte. The data reveal that the first approach is of limited applicability and separation efficiency as it provides separated transient foci of acidic ampholytes only. The second system largely extends the applicability range, separation, and sensitivity and can be applied to ampholytes with pIs as high as 10. The same is true with the base incorporated into the catholyte. Dynamic simulation provides for the first time insight into the complexity of these one-step focusing systems with buffer components that are compatible with MS detection. Simulation is an attractive and simple tool for characterization of the involved electrophoretic processes and for assay optimization.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Endogenous Polyamine Analysis via Capillary Electrophoresis/Mass Spectrometry: Characterization and Practical Considerations","authors":"Cameron J. Kaminsky,&nbsp;Jericha Mill,&nbsp;Thomas Raife,&nbsp;Lingjun Li","doi":"10.1002/elps.202400165","DOIUrl":"10.1002/elps.202400165","url":null,"abstract":"<p>Commonly used analytical techniques for polyamine analysis, including derivatization and mixed-mode liquid chromatography (LC), have inherent disadvantages. Capillary electrophoresis (CE) is uniquely suited to analyze small, highly charged molecules because analytes are separated on the basis of their electrophoretic mobility, not polarity or association with a stationary phase. Microfluidic CE–mass spectrometry (mCE–MS) is a relatively recent addition to commercially available CE offerings that streamlines traditional CE–MS interfacing and has the potential to improve upon classic CE challenges to robustness and reproducibility. MS instrument choice and scanning parameters are strongly influenced by a need for high acquisition rate to adequately sample CE peaks. Alternatively, isotachophoresis on loading can be intentionally avoided to produce sufficiently wide peaks. The mCE platform utilized here performed very well in many metrics; a limit of detection (LOD) as low as 0.25 ng/mL was achieved for spermidine, and endogenous spermidine was easily detected in blood with this method. Both of these are challenging tasks for any separation technique and demonstrate a strong use case for the platform. During experimentation, various idiosyncrasies in the commercial CE–MS interface resulted in extensive chip-to-chip variability in both peak shape and LOD, complicating the application to robust absolute quantitation. Practical guidance for similar analyses is provided.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 5-6","pages":"296-304"},"PeriodicalIF":3.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Low-Cost Microfluidic Device For the On-Line Counting of Microparticle/Bacteria","authors":"Zhen-Yu Xun,&nbsp;Lv Liu,&nbsp;Bai-Chuan Zhang,&nbsp;Liang-Liang Fan,&nbsp;Lu-Wei Zhang,&nbsp;Hong Zhao,&nbsp;Sohail Iqbal,&nbsp;Rana Iqtidar Shakoor,&nbsp;Liang Zhao","doi":"10.1002/elps.202400061","DOIUrl":"10.1002/elps.202400061","url":null,"abstract":"<div>\u0000 \u0000 <p>On-line counting of the microparticle/bacteria in the liquid medium has great potential in the food safety and biomedical fields. A new low-cost microfluidic device is proposed for the on-line counting of the microparticles/bacteria in the liquid medium. The gradually contracted microchannel and the viscoelastic fluid are combined to achieve the efficient elastic focusing of the particle/bacteria, which significantly improves the counting accuracy by aligning all particles/bacteria in a single position at the center of the microchannel. A simple light resistance-based counting method is designed and integrated with the microchannel, where the low-cost elements including the laser pointer, convex lens, diaphragm, and photodiode, are used to build the optical path of detection. The influence of the flow rate, the particle size, the property of fluid, and the channel structure on the focusing of the particle/bacteria are investigated by the experiment, and the counting ability of the integrated microfluidic device is validated by using different-sized microparticles and the <i>bifidobacterium</i>. With its simple structure, low cost, easy operation, and high efficiency, this microfluidic device is suitable for the commercial applications, such as the on-line counting of the plastic microparticle in water or the colony-forming units (CFU) of bacteria in food.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 23-24","pages":"2182-2190"},"PeriodicalIF":3.0,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electromigration of Charged Analytes Through Immiscible Fluids in Multiphasic Electrophoresis","authors":"Md Nazibul Islam,&nbsp;Yang Liu,&nbsp;Amy E. Herr","doi":"10.1002/elps.202400192","DOIUrl":"10.1002/elps.202400192","url":null,"abstract":"<p>Multiphasic buffer systems have been of greatest interest in electrophoresis and liquid–liquid electrotransfer; this study extends that foundation by exploring the interplay of the geometric and viscous properties of an interleaving oil layer on the electrotransfer of a charged analyte from an aqueous solution into a hydrogel. We utilized finite element analysis to examine two complementary configurations: one being electrotransfer of a charged analyte (protein) in an aqueous phase into a surrounding hydrogel layer and another being electrotransfer of the protein from that originating aqueous phase—through an interleaving oil layer of predetermined viscosity and thickness—and into a surrounding hydrogel layer. Results indicate that the presence of an oil layer leads to increased skew of the injected peak. To explain this difference in injection dispersion, we utilize Probstein's framework and compare the Péclet (<i>Pe</i>) number with the ratio between length scales characteristic to the axial and radial dispersion, respectively. The formulation assigns electrotransfer conditions into six different dispersion regimes. We show that the presence or absence of an interleaving oil layer moves the observed peak dispersion into distinct electrotransfer regimes; the presence of an oil layer augments the electrophoretic mobility mismatch among the different phases, resulting in a five-fold increase in <i>Pe</i> and a six-fold increase in the ratio between the axial to radial dispersion characteristic lengths. We further show that oil viscosity significantly influences resultant injection dispersion. A decrease in oil-layer viscosity from 0.08 to 0.02 Pa s results in a &gt;100% decrease in injection dispersion. Our theoretical predictions were experimentally validated by comparing the electrotransfer regimes of three different mineral oil samples. We show that lowering the oil viscosity to 0.0039 Pa s results in an injection regime similar to that of the absence of an oil layer. Understanding of the impact of electrotransfer of charged species across multiple immiscible fluid layers on peak dispersion informs the design of multiphasic electrophoresis systems.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 1-2","pages":"13-21"},"PeriodicalIF":3.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}