ELECTROPHORESIS最新文献

{"title":"Saliva Proteomics Highlights Potential BMI-Related Biomarkers for Forensic and Medical Research","authors":"Shunyi Tian,&nbsp;Qiyan Wang,&nbsp;Xiaolan Huang,&nbsp;Shengjie Zhu,&nbsp;Hongling Zhang,&nbsp;Zheng Ren,&nbsp;Yubo Liu,&nbsp;Meiqing Yang,&nbsp;Jiang Huang,&nbsp;Xiaoye Jin","doi":"10.1002/elps.202400206","DOIUrl":"10.1002/elps.202400206","url":null,"abstract":"<div>\u0000 \u0000 <p>Obesity and underweight pose significant threats to human health as non-communicable diseases. In addition, body shape (like obesity or emaciation) is an important investigative clue in forensic practice. Body mass index (BMI) is a common indicator to reflect body shape of an individual. However, there is a lack of rapid, simple, and effective methods for identifying different BMI individuals. This research aimed to delve into the correlations between salivary proteins and BMI. A total of 418 differential expression proteins (DEPs) were identified through four-dimensional data independent acquisition quantitative proteomics analysis. The Kyoto Encyclopedia of Genes and Genomes analysis revealed that DEPs were primarily involved in oxidative phosphorylation, protein processing in the endoplasmic reticulum, and cholesterol metabolism pathways. Finally, we identified 17 protein markers that were correlated with BMI. Two machine-learning models (random forest and support vector machine) were also built based on these 17 markers. Obtained results demonstrated the efficacy of these 17 protein markers in accurately distinguishing different BMI individuals. In conclusion, our study not only provides potential salivary protein markers for identifying obesity and underweight individuals, it could also present a novel method for inferring BMI of saliva-related samples in forensic case investigation.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 3-4","pages":"250-257"},"PeriodicalIF":3.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surfactant-Based Polymer Microchip Electrophoresis of Ciprofloxacin Hydrochloride Monohydrate in Unfiltered Milk With Fluorescence Detection","authors":"Camille A. Leclerc,&nbsp;Christopher G. D. Ty,&nbsp;Sean S. Worthington,&nbsp;Malley B. Richardson,&nbsp;Abdulla K. AlSawalhi,&nbsp;Larry Wood,&nbsp;Khalid Moomand,&nbsp;Christopher M. Collier","doi":"10.1002/elps.202400079","DOIUrl":"10.1002/elps.202400079","url":null,"abstract":"<p>This work describes a cross-shaped PMMA ME system capable of detecting ciprofloxacin hydrochloride monohydrate (CPFH) in unfiltered milk samples. The cross-shaped PMMA ME system utilizes a BGE consisting primarily of the surface-active agent SDS to solubilize milk fat and improve the zeta potential of the PMMA microchannel surface. A theoretical lumped-element circuit model for cross-shaped ME is introduced in this work to calculate the migration time of CPFH. This manuscript improves the capabilities of PMMA-based ME for CPFH in milk using an SDS-based BGE. The presented ME system has a faster migration time, higher mean output voltage, and thinner full-width at half-maximum than previously reported dairy-based biosensor systems. Most notably, the migration time of the new system is under 10 min, being the time associated with the milking of cattle. The system is also found to be able to detect the presence of milk fat. Discussion is included of potential future integration with existing high-sensitivity methodologies to place the overall ME system's limit of detection below an established target. To the authors’ knowledge, this is the first reported account of a PMMA ME system capable of detecting CPFH in unfiltered milk.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 3-4","pages":"143-151"},"PeriodicalIF":3.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lorenz Model for Chaos in Electrokinetic Instability.","authors":"Prateek Gupta, Supreet Singh Bahga","doi":"10.1002/elps.202400203","DOIUrl":"https://doi.org/10.1002/elps.202400203","url":null,"abstract":"<p><p>We demonstrate that the Lorenz system of equations can approximate the nonlinear flow dynamics of the electrokinetic instability (EKI) in a microchannel driven by an electric field applied parallel to the diffusive interfaces separating the co-flowing centre and sheath streams with mismatched electrical conductivity. Using Galerkin projection, we show that the electrohydrodynamic flow equations can be approximated by the Lorenz equations in the limit of small conductivity difference between the flow streams. The derived dynamical model qualitatively captures the characteristics of EKI in both linear and nonlinear regimes, including the neutral stability criterion and alternating transitions between periodic and aperiodic states with increasing electric Rayleigh numbers. While not quantitatively precise, this simplified dynamical model provides valuable insights into the essential nonlinearities responsible for chaotic behaviour observed in EKI experiments.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Strategy for Authentication and Quantification of Porcine and Water Buffalo Hide Gelatin in Processed Products Using Multiple Reaction Monitoring Mass Spectrometry Approaches","authors":"Bidyut Prava Mishra,&nbsp;Naveena B. Maheswarappa,&nbsp;B. Eswara Rao,&nbsp;Rituparna Banerjee,&nbsp;Prasad M. Govindaiah,&nbsp;Balaji B. Manohar,&nbsp;Prasana Kumar Rath","doi":"10.1002/elps.202400209","DOIUrl":"10.1002/elps.202400209","url":null,"abstract":"<div>\u0000 \u0000 <p>Pig skin and water buffalo hides were partially hydrolyzed to produce a heterogeneous mixture of polypeptides, known as gelatin, a commonly used food additive. Specific peptide markers were explored in the context of authentication and relative quantification of water buffalo hide gelatin (BHG) and porcine skin gelatin (PSG) via multiple reaction monitoring-mass spectrometry (MRM-MS). Tryptic gelatin peptides were separated and analyzed with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and five unique peptide biomarkers were selected for BHG and PSG. Gelatin extraction from BHG and PSG spiked chicken meat patties (CMP) followed by LC-MS/MS MRM-MS revealed two unique bovine gelatin peptides of <i>m</i>/<i>z</i> 781.336 and 852.718 and three unique porcine gelatin peptides of <i>m</i>/<i>z</i> 774.570, 971.776, and 727.436. The optimized MRM-MS protocol was efficient in detecting BHG/PSG from spiked CMP up to 0.5% (w/w). Twenty-five commercial food samples were screened, among which four samples showed the presence of BHG and six samples showed the presence of PSG. The proposed LC-MS/MS MRM strategy provides an efficient and sensitive authentication and traceability of gelatin-containing highly processed food, bakery, and confectionery products.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 5-6","pages":"331-339"},"PeriodicalIF":3.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board: Electrophoresis 1–2'25","authors":"","doi":"10.1002/elps.202570011","DOIUrl":"https://doi.org/10.1002/elps.202570011","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 1-2","pages":"1-2"},"PeriodicalIF":3.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202570011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143119695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contents: Electrophoresis 1–2'25","authors":"","doi":"10.1002/elps.202570012","DOIUrl":"https://doi.org/10.1002/elps.202570012","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 1-2","pages":"3-4"},"PeriodicalIF":3.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143119696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances and Applications of Capillary Electrophoresis Mass Spectrometry in Food Analysis: Strategies for Online and Offline Preconcentration","authors":"Nadhiratul-Farihin Semail,&nbsp;Noorfatimah Yahaya,&nbsp;Ahmad Husaini Mohamed,&nbsp;David Da Yong Chen,&nbsp;Nur Nadhirah Mohamad Zain","doi":"10.1002/elps.202400211","DOIUrl":"10.1002/elps.202400211","url":null,"abstract":"<div>\u0000 \u0000 <p>Advancements in food technology have increased the need for thorough analysis to ensure food safety, quality, and compliance with regulatory requirements. Capillary electrophoresis–mass spectrometry (CE–MS) has emerged as a powerful tool in food analysis due to its high separation efficiency, low sample consumption, and ability to handle complex matrices. However, challenges such as the use of volatile running buffers and maintaining the stability of the electrical circuit connecting the CE and MS systems have been addressed through advancements in interface designs, such as sheathless systems and optimized sheath-liquid compositions. Online and offline preconcentration techniques have significantly enhanced CE–MS sensitivity (up to 1000-fold) through stacking methods such as large volume sample stacking (LVSS) and dynamic pH junction stacking. Meanwhile, offline sample preparation techniques, such as solid-phase extraction (SPE) and liquid-based methods, are essential for removing matrix interferences and preconcentrating targeted analytes. This review explores both online and offline preconcentration methods and emphasizes the importance of CE–MS in helping researchers develop effective strategies for selecting the best preconcentration methods for food analysis.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 5-6","pages":"263-278"},"PeriodicalIF":3.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal Analysis of Electromagnetic Induction Heating for Cylinder-Shaped Objects.","authors":"Amir Komeili Birjandi, Prashanta Dutta","doi":"10.1002/elps.202400216","DOIUrl":"https://doi.org/10.1002/elps.202400216","url":null,"abstract":"<p><p>Induction heating is one of the cleanest and most efficient methods for heating materials, utilizing electromagnetic fields induced through AC electric current. This article reports an analytical solution for transient heat transfer in a three-dimensional (3D) cylindrical object under induction heating. A simplified form of Maxwell's equations is solved to determine the heat generation inside the cylinder by calculating the current density distribution within the body. The temperature within the solid is found from the solution of the unsteady heat equation based on Green's function. Owing to multiple spatial dimensions and time, a separation of variables technique is used to find Green's function. In addition, an innovative algorithm is proposed to take care of the variable material properties in analytical treatment. The analytical solution for temperature is verified with the data obtained from experiments for identical operating conditions. The analytical solution is used to study the impact of heat transfer coefficient and input AC current frequency and amplitude during transient heat diffusion. Our analytical solution suggests that the temperature-dependent material properties significantly affect the thermal response within the solid. Unlike many other conventional heating methods, the thermal boundary condition changes with time in induction heating, which makes our solution much more challenging.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enabling icIEF Peak Identification of AAV Capsid Proteins by Fractionation on MauriceFlex and Subsequent Analysis by LC–MS","authors":"Will McElroy,&nbsp;Sisi Huang,&nbsp;Xiaoping He,&nbsp;Cheng Zhou,&nbsp;Christopher D. Heger,&nbsp;Thomas W. Powers,&nbsp;Melissa M. Anderson,&nbsp;Courtney Sloan,&nbsp;Thomas F. Lerch","doi":"10.1002/elps.202400201","DOIUrl":"10.1002/elps.202400201","url":null,"abstract":"<p>A significant limitation of imaged capillary electric focusing (icIEF) is the inability to identify and characterize specific species in the electropherogram. This has led to the development of complementary ion-exchange chromatography (IEX)-based methods that are amenable to either fraction collection and subsequent characterization or online IEX coupled to mass spectrometry. To overcome this limitation while maintaining the use of icIEF, novel approaches, including an icIEF separation and fractionation technology (MauriceFlex, ProteinSimple), have been developed. This approach enables the fractionation of various icIEF peaks, which can then be characterized by mass spectrometry to confirm the identity of the separated charged species. Herein, the MauriceFlex technology was applied to adeno-associated viral (AAV) gene therapy products, which contain a DNA transgene packaged into a protein capsid and have shown tremendous therapeutic potential in recent years. Utilizing the MauriceFlex system, we developed an approach for the separation of charged species from AAV capsid viral proteins (VP) by icIEF and subsequent characterization by liquid chromatography and mass spectrometry (LC–MS). When applying the same sample preparation, charge profiles of AAV capsid proteins on the MauriceFlex instrument were demonstrated to be consistent with those from the original Maurice platform, the industrial gold standard. Optimization of the VP icIEF fractionation method required the development of a method for low concentration samples, optimization of mobilization conditions, enhancement of fraction recovery, and maintenance of protein stability post fractionation. Herein, we were able to successfully collect charge-separated VP fraction samples and subsequently analyze them by MS analysis. In addition, a workflow for AAV capsid protein characterization based on icIEF separation and fractionation coupled with downstream LC–MS has been established for the confirmation of VP identity and additional characterization of capsid protein heterogeneity.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 1-2","pages":"22-33"},"PeriodicalIF":3.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatio-Temporal Change of Skin and Oral Microbiota: A Longitudinal Study of Microbial Diversity and Stability","authors":"Han Zhang,&nbsp;Anqi Chen,&nbsp;Shilin Li,&nbsp;Kaiqin Chen,&nbsp;Xuechun You,&nbsp;Yingnan Bian,&nbsp;Chengtao Li,&nbsp;Shiquan Liu,&nbsp;Jiang Huang,&nbsp;Suhua Zhang","doi":"10.1002/elps.202400160","DOIUrl":"10.1002/elps.202400160","url":null,"abstract":"<div>\u0000 \u0000 <p>The human skin and oral cavity harbor complex microbial communities, which exist in dynamic equilibrium with the host's physiological state and the external environment. This study investigates the microbial atlas of human skin and oral cavities using samples collected over a 10-month period, aiming to assess how both internal and external factors influence the human microbiome. We examined bacterial community diversity and stability across various body sites, including palm and nasal skin, saliva, and oral epithelial cells, during environmental changes and a COVID-19 pandemic. The skin microbiome was confirmed to display spatial and temporal stability compared to the oral microbiome, particularly the oral epithelium, which was susceptible to changes in the host's physiological state and immune response. Moreover, significant differences in the microbial community structure among the 4 sample types were observed, and 87 distinct bacteria biomarkers were identified. The random forest prediction model achieved an overall prediction accuracy of 95.24% across the four types of samples studied. Additionally, nasal skin samples showed significant promise for individual identification through profiling the skin microbiota. These findings highlight the potential of skin and oral microbiota as forensic markers for inferring body sites and identifying individuals. In summary, despite facing limitations such as a small cohort size and the need for broader validation, this research provides an overall perspective and initial insights for refining experimental designs and conducting in-depth research in various microbial research fields.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 1-2","pages":"92-103"},"PeriodicalIF":3.0,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}