ELECTROPHORESIS最新文献_第7页

Oligo cyc-DEP: On-chip cyclic immunofluorescence profiling of cell-derived nanoparticles Oligo cyc-DEP：细胞衍生纳米颗粒的片上循环免疫荧光分析。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-25 DOI: 10.1002/elps.202400088

Kyle T. Gustafson, Zeynep Sayar, Augusta Modestino, Hillary H. Le, Austin Gower, Fehmi Civitci, Sadik C. Esener, Michael J. Heller, Sebnem Ece Eksi

{"title":"Oligo cyc-DEP: On-chip cyclic immunofluorescence profiling of cell-derived nanoparticles","authors":"Kyle T. Gustafson, Zeynep Sayar, Augusta Modestino, Hillary H. Le, Austin Gower, Fehmi Civitci, Sadik C. Esener, Michael J. Heller, Sebnem Ece Eksi","doi":"10.1002/elps.202400088","DOIUrl":"10.1002/elps.202400088","url":null,"abstract":"We present a follow-on technique for the cyclic-immunofluorescence profiling of suspension particles isolated using dielectrophoresis. The original lab-on-chip technique (“cyc-DEP” [cyclic immunofluorescent imaging on dielectrophoretic chip]) was designed for the multiplex surveillance of circulating biomarkers. Nanoparticles were collected from low-volume liquid biopsies using microfluidic dielectrophoretic chip technology. Subsequent rounds of cyclic immunofluorescent labeling and quenching were imaged and quantified with a custom algorithm to detect multiple proteins. While cyc-DEP improved assay multiplicity, long runtimes threatened its clinical adoption. Here, we modify the original cyc-DEP platform to reduce assay runtimes. Nanoparticles were formulated from human prostate adenocarcinoma cells and collected using dielectrophoresis. Three proteins were labeled on-chip with a mixture of short oligonucleotide-conjugated antibodies. The sample was then incubated with complementary fluorophore-conjugated oligonucleotides, which were dehybridized using an ethylene carbonate buffer after each round of imaging. Oligonucleotide removal exhibited an average quenching efficiency of 98 ± 3% (n = 12 quenching events), matching the original cyc-DEP platform. The presented “oligo cyc-DEP” platform achieved clinically relevant sample-to-answer times, reducing the duration for three rounds of cyclic immunolabeling from approximately 20 to 6.5 h—a 67% decrease attributed to rapid fluorophore removal and the consolidated co-incubation of antibodies.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141757843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and adsorption of florfenicol in milk using bifunctional carbon dot-doped molecularly imprinted polymers. 使用双功能碳点掺杂分子印迹聚合物检测和吸附牛奶中的氟苯尼考。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-22 DOI: 10.1002/elps.202400053

Weiyan Li, Chuansheng Sun, Haiping Wang, Qingyan Bai, Yi Xu, Chunmiao Bo, Junjie Ou

{"title":"Detection and adsorption of florfenicol in milk using bifunctional carbon dot-doped molecularly imprinted polymers.","authors":"Weiyan Li, Chuansheng Sun, Haiping Wang, Qingyan Bai, Yi Xu, Chunmiao Bo, Junjie Ou","doi":"10.1002/elps.202400053","DOIUrl":"https://doi.org/10.1002/elps.202400053","url":null,"abstract":"Detection of florfenicol (FF) residues in animal-derived foods, as one of the most widely used antibiotics, is critically important to food safety. The fluorescent molecularly imprinted polymer (MIP) was synthesized by surface-initiated atom transfer radical polymerization technique with poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) microspheres, 4-vinylpyridine, ethylene glycol dimethacrylate, and FF as the matrix, functional monomer, crosslinker, and template molecule, respectively. Meanwhile, N-S co-doped carbon dot (CD) was synthesized with triammonium citrate and thiourea as precursors under microwave irradiation at 400 W for 2.5 min and then integrated into FF-MIP to obtain CD@FF-MIP. For comparison, non-imprinted polymer (NIP) without FF was also prepared. The adsorption capacity of CD@FF-MIP to FF reached 53.1 mg g-1, which was higher than that of FF-MIP (34.7 mg g-1), whereas the adsorption capacity of NIP was only 17.3 mg g-1. The adsorption equilibrium of three materials was reached within 50 min. Particularly, CD@FF-MIP exhibited an excellent fluorescence quenching response to FF in the concentration range of 3-50 µmol L-1. As a result, CD@FF-MIP was successfully utilized to extract FF in milk samples, which were analyzed by high-performance liquid chromatography. The standard recoveries were 95.8%-98.2%, and the relative standard deviation was 1.6%-4.2%. The method showed the advantages of simple operation, high sensitivity, excellent selectivity, and low cost, and also demonstrated a great application prospect in food detection.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contents: Electrophoresis 13–14'24 内容：电泳 13-14'24

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-12 DOI: 10.1002/elps.202470073

引用次数: 0

Editorial Board: Electrophoresis 13–14'24 编辑委员会：电泳 13-14'24

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-12 DOI: 10.1002/elps.202470072

引用次数: 0

Using rDNA ITS2 barcoding to identify kratom (Mitragyna speciosa) from the genus Mitragyna and Neolamarckia cadamba. 利用 rDNA ITS2 条形码从 Mitragyna 和 Neolamarckia cadamba 属中识别桔梗（Mitragyna speciosa）。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-11 DOI: 10.1002/elps.202400003

Meng-Yi Chen, Yu-Ching Tu, Hsin-Yi Shyu, Ting-An Lin, Chun-Pai Juan, Fang-Chin Wu

{"title":"Using rDNA ITS2 barcoding to identify kratom (Mitragyna speciosa) from the genus Mitragyna and Neolamarckia cadamba.","authors":"Meng-Yi Chen, Yu-Ching Tu, Hsin-Yi Shyu, Ting-An Lin, Chun-Pai Juan, Fang-Chin Wu","doi":"10.1002/elps.202400003","DOIUrl":"https://doi.org/10.1002/elps.202400003","url":null,"abstract":"This study collected 80 samples of suspected kratom plant powder. A polymerase chain reaction sequence analysis was conducted using two sets of DNA barcode primers for plant ribosomal (r)DNA internal transcribed spacers (ITSs), namely, ITS3/ITS4 and ITS-p3/ITS-u4. Among the 80 samples, 40 were analyzed using the ITS3/ITS4 primer pair, and then DNA sequences were subjected to a National Center for Biotechnology Information-Basic Local Alignment Search Tool (NCBI-BLAST) comparison. Results showed that 29 samples had a 100% match (364/364) with Mitragyna speciosa (kratom), and 6 samples had a 99.73% match (363/364) with M. speciosa, whereas 5 samples had disordered and unreadable sequences. The 5 unreadable samples and an additional 40 suspected kratom samples were then analyzed using the ITS-p3/ITS-u4 primer pair, followed by an NCBI-BLAST comparison. Among these, 32 samples had a 100% match (404/404) with M. speciosa, and 11 samples had a 99.75% match (403/404) with M. speciosa. Among the samples with sequences matching M. speciosa, three distinct types were observed (no variance/404, 287M/404, and 287A/404). One sample had a 99.51% match (404/406) with Neolamarckia cadamba, and another sample had a sequencing length of 305 bp, with 25 positions showing mixed base pairs, indicating a mixture of different species. Analysis of the mixed base pair pattern suggested a possible mixture of M. speciosa and N. cadamba. Actually, M. speciosa and N. cadamba have very similar external morphologies. This indicates that the ITS-p3/ITS-u4 primer pair is effective in distinguishing mixtures of M. speciosa and N. cadamba and is thus more suitable than ITS3/ITS4 for identifying and analyzing samples of suspected kratom plant powder.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison between capillary electrophoresis and fluorescence anisotropy competitive immunoassay for glucagon 毛细管电泳与荧光各向异性竞争免疫测定法检测胰高血糖素的比较。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-10 DOI: 10.1002/elps.202400080

Yao Wang, Emily L. Skinner, Michael G. Roper

{"title":"Comparison between capillary electrophoresis and fluorescence anisotropy competitive immunoassay for glucagon","authors":"Yao Wang, Emily L. Skinner, Michael G. Roper","doi":"10.1002/elps.202400080","DOIUrl":"10.1002/elps.202400080","url":null,"abstract":"Glucagon plays a crucial role in regulating glucose homeostasis; unfortunately, the mechanisms controlling its release are still unclear. Capillary electrophoresis (CE)- and fluorescence anisotropy (FA)-immunoassays (IA) have been used for online measurements of hormone secretion on microfluidic platforms, although their use in glucagon assays is less common. We set out to compare a glucagon-competitive IA using these two techniques. Theoretical calibration curves were generated for both CE- and FA–IA and results indicated that CE-IA provided higher sensitivity than FA–IA. These results were confirmed in an experiment where both assays showed limits of detection (LOD) of 30 nM, but the CE-IA had ∼300-fold larger sensitivity from 0 to 200 nM glucagon. However, in online experiments where reagents were mixed within the device, the sensitivity of the CE-IA was reduced ∼3-fold resulting in a higher LOD of 70 nM, whereas the FA–IA remained essentially unchanged. This lowered sensitivity in the online CE-IA was likely due to poor sampling by electroosmotic flow from the high salt solution necessary in online experiments, whereas pressure-based sampling used in FA–IA was not affected. We conclude that FA–IA, despite lowered sensitivity, is more suitable for online mixing scenarios due to the ability to use pressure-driven flow and other practical advantages such as the use of larger channels.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possibility of using the imaged capillary isoelectric method as a multi-attribute method for bispecific antibodies 将成像毛细管等电法用作双特异性抗体多属性方法的可能性。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-08 DOI: 10.1002/elps.202400066

Gang Wu, Jialiang Du, Gangling Xu, Meng Li, Chuanfei Yu

引用次数: 0

Enantiomeric separation of nefopam and cathinone derivatives using a supramolecular deep eutectic solvent as a chiral selector in capillary electrophoresis 在毛细管电泳中使用超分子深共晶溶剂作为手性选择器分离奈福泮和卡西酮衍生物的对映体。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-04 DOI: 10.1002/elps.202400092

Katerina A. Ioannou, Maria N. Georgiou, Georgia D. Ioannou, Atalanti Christou, Ioannis J. Stavrou, Martin G. Schmid, Constantina P. Kapnissi-Christodoulou

{"title":"Enantiomeric separation of nefopam and cathinone derivatives using a supramolecular deep eutectic solvent as a chiral selector in capillary electrophoresis","authors":"Katerina A. Ioannou, Maria N. Georgiou, Georgia D. Ioannou, Atalanti Christou, Ioannis J. Stavrou, Martin G. Schmid, Constantina P. Kapnissi-Christodoulou","doi":"10.1002/elps.202400092","DOIUrl":"10.1002/elps.202400092","url":null,"abstract":"The present study investigates the utilization of a supramolecular deep eutectic solvent (SUPRADES), consisting of sulfated-β-cyclodextrin (S-β-CD) and citric acid (CA), as a chiral selector (CS) in capillary electrophoresis for the enantiomeric separation of nefopam (NEF) and five cathinone derivatives (3-methylmethcathinone [3-MMC], 4-methylmethcathinone [4-MMC], 3,4-dimethylmethcathinone [3,4-DMMC], 4-methylethcathinone [4-MEC], and 3,4-methylendioxycathinone [MDMC]). A significant improvement in enantiomeric separation of the target analytes was observed upon the addition of S-β-CD-CA to the background electrolyte (BGE), leading to a baseline separation of all analytes. In particular, the optimum percentage of S-β-CD-CA, added to the BGE, was determined to be 0.075% v/v for NEF (Rs = 1.5) and 0.050% v/v for three out of five cathinone derivatives (Rs = 1.5, 1.6, and 2.4 for 3-MMC, 4-MEC, and 3,4-DMMC, respectively). In the case of 4-MMC and MDMC, a higher percentage of the CS, equal to 0.075% and 0.10% v/v, respectively, was required to achieve baseline separation (Rs = 1.5, 1.9 for MDMC and 4-MMC, respectively). The outcomes of the present study highlight the potential effectiveness of using SUPRADES as a CS in electrophoretic enantioseparations.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing water hyacinth plants from two South African rivers for the detection of seven pharmaceuticals and their metabolites. 分析南非两条河流中的水葫芦植物，以检测七种药物及其代谢物。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-04 DOI: 10.1002/elps.202400101

Markus Himmelsbach, Franz Mlynek, Wolfgang Buchberger, Lawrence Madikizela, Christian W Klampfl

引用次数: 0

Local nano-electrode fabrication utilizing nanofluidic and nano-electrochemical control. 利用纳米流体和纳米电化学控制制造局部纳米电极。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-04 DOI: 10.1002/elps.202300002

Kyojiro Morikawa, Tomoaki Takeuchi, Takehiko Kitamori

{"title":"Local nano-electrode fabrication utilizing nanofluidic and nano-electrochemical control.","authors":"Kyojiro Morikawa, Tomoaki Takeuchi, Takehiko Kitamori","doi":"10.1002/elps.202300002","DOIUrl":"https://doi.org/10.1002/elps.202300002","url":null,"abstract":"Miniaturized systems have attracted much attention with the recent advances in microfluidics and nanofluidics. From the capillary electrophoresis, the development of glass-based microfluidic and nanofluidic technologies has supported advances in microfluidics and nanofluidics. Most microfluidic systems, especially nanofluidic systems, are still simple, such as systems constructed with simple straight nanochannels and bulk-scale electrodes. One of the bottlenecks to the development of more complicated and sophisticated systems is to develop the locally integrated nano-electrodes. However, there are still issues with integrating nano-electrodes into nanofluidic devices because it is difficult to fit the nano-electrode size into a nanofluidic channel at the nanometer level. In this study, we propose a new method for the fabrication of local nano-electrodes in nanofluidic devices with nanofluidic and nano-electrochemistry-based experiments. An electroplating solution was introduced to a nanochannel with control of the flow and the electroplating reaction, by which nano-electrodes were successfully fabricated. In addition, a nanofluidic device was available for nanofluidic experiments with the application of 200 kPa. This method can be applied to any electroplating material such as gold and copper. The local nano-electrode will make a significant contribution to the development of more complicated and sophisticated nanofluidic electrophoresis systems and to local electric detection methods for various nanofluidic devices.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0