ELECTROPHORESIS最新文献_第7页

Charge variant analysis of monoclonal antibodies by CZE‐MS using a successive multiple ionic‐polymer layer coating based on diethylaminoethyl‐dextran 利用基于二乙氨基乙基葡聚糖的连续多重离子聚合物层涂层，通过 CZE-MS 对单克隆抗体进行电荷变异分析

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-17 DOI: 10.1002/elps.202400084

Alisa Höchsmann, Laura Dhellemmes, Laurent Leclercq, Hervé Cottet, Christian Neusüß

{"title":"Charge variant analysis of monoclonal antibodies by CZE‐MS using a successive multiple ionic‐polymer layer coating based on diethylaminoethyl‐dextran","authors":"Alisa Höchsmann, Laura Dhellemmes, Laurent Leclercq, Hervé Cottet, Christian Neusüß","doi":"10.1002/elps.202400084","DOIUrl":"https://doi.org/10.1002/elps.202400084","url":null,"abstract":"The characterization of the impurities of pharmaceutical monoclonal antibodies (mAbs) is crucial for their function and safety. Capillary zone electrophoresis (CZE) is one of the most efficient tools to separate charge variants of mAbs; however, peak characterization remains difficult, since the hereby used background electrolytes (BGEs) are not compatible with electrospray ionization‐mass spectrometry (ESI‐MS). Here, a method that allows the separation of intact mAb charge variants is presented using CZE‐ESI‐MS, combining a cationic capillary coating and an acidic BGE. Therefore, a successive multiple ionic‐polymer layer coating was developed based on diethylaminoethyl‐dextran–poly(sodium styrene sulfonate). This coating leads to a relatively low reversed electroosmotic flow (EOF) with an absolute mobility slightly higher than that of antibodies, enabling the separation of variants with slightly different mobilities. The potential of the coating is demonstrated using USP mAb003, where it was possible to separate C‐terminal lysine variants from the main form, as well as several acidic variants and monoglycosylated mAb forms. The presented CZE‐MS method can be applied to separate charge variants of a range of other antibodies such as infliximab, NISTmAB (Reference Material from the National Institute of Standards and Technology), adalimumab, and trastuzumab, demonstrating the general applicability for the separation of proteoforms of mAbs.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"16 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streaming electric field, electroviscous effect, and electrokinetic liquid flows in the induced pressure‐driven transport of active liquids in narrow capillaries 窄毛细管中活性液体的诱导压力驱动输送中的流动电场、电粘性效应和电动液体流

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-17 DOI: 10.1002/elps.202400133

Siddhartha Das, Ghansham Rajendrasingh Chandel

{"title":"Streaming electric field, electroviscous effect, and electrokinetic liquid flows in the induced pressure‐driven transport of active liquids in narrow capillaries","authors":"Siddhartha Das, Ghansham Rajendrasingh Chandel","doi":"10.1002/elps.202400133","DOIUrl":"https://doi.org/10.1002/elps.202400133","url":null,"abstract":"In this paper, we develop a theory for studying the electrokinetic effects in a charged nanocapillary filled with active liquid. The active particles present within the active liquid are self‐driven, demonstrate vortex defects, and enforce a circumferentially arranged polarization field. Under such circumstances, there is the development of an induced pressure‐gradient‐driven transport dictated (similar to diffusioosmotic transport) by the presence of an axial gradient in the activity (or the concentration of the active particles). This pressure‐driven transport has a profile different from the standard Hagen–Poiseuille flow in a nanocapillary. Also, this induced pressure‐driven flow drives electrokinetic effects, which are characterized by the generation of a streaming electric field, associated electroosmotic (EOS) transport opposing pressure‐driven flow, and electroviscous effect. We quantify these effects as functions of dimensionless parameters that vary inversely as the strength of the activity‐induced pressure‐driven flow and salt concentrations. Overall, we anticipate that this paper will draw immense attention toward a new type of activity‐induced pressure‐driven flow and associated electrokinetic phenomena in charged nanoconfinements.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"75 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophoresis of polyelectrolyte‐adsorbed soft particle with hydrophobic inner core 具有疏水内核的聚电解质吸附软颗粒的电泳技术

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-17 DOI: 10.1002/elps.202400143

Asim Mahata, Sanjib Kumar Pal, Hiroyuki Ohshima, Partha P. Gopmandal

{"title":"Electrophoresis of polyelectrolyte‐adsorbed soft particle with hydrophobic inner core","authors":"Asim Mahata, Sanjib Kumar Pal, Hiroyuki Ohshima, Partha P. Gopmandal","doi":"10.1002/elps.202400143","DOIUrl":"https://doi.org/10.1002/elps.202400143","url":null,"abstract":"This article deals with the electrophoresis of hydrophobic colloids absorbed by a layer of polymers with an exponential distribution of the polymer segments. The functional groups present in the polymer layer further follow the exponential distribution. We made an extensive mathematical study of the electrophoresis of such core‐shell structured soft particles considering the combined impact of heterogeneity in polymer segment distribution, ion steric effect, and hydrodynamic slippage of the inner core. The mathematical model is based on the flat‐plate formalism and deduced numerical results for electrophoretic mobility are valid for weak to highly charged particles for which the particle size well exceeds the Debye‐layer thickness. In addition, we have derived closed form analytical results for electrophoretic mobility of the particle under several electrohydrodynamic limits. We have further illustrated the results for electrophoretic mobility considering a charged and hydrophobic inner core coated with an uncharged polymer layer or a polymer layer that entraps either positive or negatively charged functional groups. The impact of pertinent parameters on the overall electrophoretic motion is further illustrated.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"40 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrokinetic particle trapping in microfluidic wells using conductive nanofiber mats. 利用导电纳米纤维毡在微流孔中进行电动粒子捕集。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-09-02 DOI: 10.1002/elps.202400051

J Hunter West, Tonoy K Mondal, Stuart J Williams

引用次数: 0

Miniaturized nanoelectrospray interface for coupling capillary electrophoresis with mass spectrometry detection 用于毛细管电泳与质谱检测耦合的微型纳米电喷雾接口。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-08-23 DOI: 10.1002/elps.202400090

Tomáš Václavek, Elizaveta Vereshchagina, Leny Nazareno, Anand Summanwar, František Foret, Roman Řemínek