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Recent Progress and Applications of Advanced Nanomaterials in Solid-Phase Extraction. 先进纳米材料在固相萃取中的最新进展和应用。
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-11-05 DOI: 10.1002/elps.202400152
Pin Chen, Qiuyue Zhang, Hang Yin, Siyuan Di, Huan Liu, Hailan Qin, Ming Liu, Yunkang Liu, Zihan Li, Shukui Zhu
{"title":"Recent Progress and Applications of Advanced Nanomaterials in Solid-Phase Extraction.","authors":"Pin Chen, Qiuyue Zhang, Hang Yin, Siyuan Di, Huan Liu, Hailan Qin, Ming Liu, Yunkang Liu, Zihan Li, Shukui Zhu","doi":"10.1002/elps.202400152","DOIUrl":"https://doi.org/10.1002/elps.202400152","url":null,"abstract":"<p><p>Sample preparation maintains a key bottleneck in the whole analytical procedure. Solid-phase sorbents (SPSs) have garnered increasing attention in sample preparation research due to their crucial roles in achieving high clean-up and enrichment efficiency in the analysis of trace targets present in complex matrices. Novel nanoscale materials with improved characteristics have garnered considerable interest across different scientific disciplines due to the limited capabilities of traditional bulk-scale materials. The purpose of this review is to offer a thorough summary of the latest developments and uses of SPSs in preparing samples for chromatographic analysis, focusing on the years 2020-2024. The techniques for preparing SPSs are examined, such as metal-organic frameworks (MOFs), covalent organic frameworks (COFs), carbon nanoparticles (CNPs), molecularly imprinted polymers (MIPs), and metallic nanomaterials (MNs). Examining the pros and cons of different extraction methods, including solid-phase extraction (SPE), magnetic SPE (MSPE), flow-based SPE (FBA-SPE), solid-phase microextraction (SPME), stir-bar sorptive extraction (SBSE), and dispersive SPE (DSPE), is the main focus. Furthermore, this article presents the utilization of SPE technology for isolating common contaminants in various environmental, biological, and food specimens. We highlight the persistent challenges in SPSs and anticipate future advancements and applications of novel SPSs.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer-Modified Magnetic Beads and Permalloy Micromagnets 使用 Aptamer 改性磁珠和坡莫合金微磁体从混合细胞样本中分离肺癌细胞
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-11-05 DOI: 10.1002/elps.202400147
Shu-Hui Lin, Yun-Jung Tsai, Tzu-Cheng Su, Shih-Lun Lai, Chun-Ping Jen
{"title":"Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer-Modified Magnetic Beads and Permalloy Micromagnets","authors":"Shu-Hui Lin,&nbsp;Yun-Jung Tsai,&nbsp;Tzu-Cheng Su,&nbsp;Shih-Lun Lai,&nbsp;Chun-Ping Jen","doi":"10.1002/elps.202400147","DOIUrl":"10.1002/elps.202400147","url":null,"abstract":"<div>\u0000 \u0000 <p>This study involved the design and fabrication of a microfluidic chip integrated with permalloy micromagnets. The device was used with aptamer-modified magnetic beads (MBs) of various sizes to successfully separate lung cancer cells from a mixture of other cells. The overall separation efficiency was evaluated based on the ratios of cells in the different outlets and inlets of the chip. The results showed efficiencies ranging from 43.4% to 50.2% for MB sizes between 1.36 and 4.50 µm. Interestingly, efficiency slightly decreased as the size of the MBs increased, contrary to predictions. Further examination revealed that larger MBs exerted gravitational force on the cell-bound MBs at low flow rates, causing the targets to settle before reaching the main microchannel region. This was attributed to fluidic resistance caused by a size mismatch between the inlet tube and the microfluidic conduit. An increase in cell accumulation at the inlet was observed with larger MB sizes due to gravity. Therefore, the definition of effective separation efficiency was revised to exclude the effect of cell accumulation at the inlet. Effective separation efficiencies were found to be 71.6%, 76.4%, and 79.4% for MB sizes of 1.36, 3.00, and 4.50 µm, respectively. The study concluded that larger MBs interacted more with the magnetic force, resulting in better separation. However, cells with smaller MBs were more likely to evade the magnetic force. The investigation provides valuable insights into isolating lung cancer cells using this method, with the potential for clinical application in cancer diagnosis and treatment.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 21-22","pages":"2054-2064"},"PeriodicalIF":3.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electroextraction of Large Volume Samples Using Paper Points Coupled With Hollow Fiber Membranes: Study of Parameters and Strategies to Enhance Analytical Performance. 使用纸点和中空纤维膜对大容量样品进行电萃取:提高分析性能的参数和策略研究。
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-10-29 DOI: 10.1002/elps.202400102
Ricardo Mathias Orlando, Miloš Dvořák, Pavel Kubáň
{"title":"Electroextraction of Large Volume Samples Using Paper Points Coupled With Hollow Fiber Membranes: Study of Parameters and Strategies to Enhance Analytical Performance.","authors":"Ricardo Mathias Orlando, Miloš Dvořák, Pavel Kubáň","doi":"10.1002/elps.202400102","DOIUrl":"https://doi.org/10.1002/elps.202400102","url":null,"abstract":"<p><p>Electroextraction (EE) encompasses a range of sample preparation methods whose effectiveness, selectivity, and efficiency are significantly influenced by the physical-chemical characteristics of analytes, samples, and instrumental conditions. This article explores, for the first time, various strategies aimed at enhancing the extraction efficiency of a recent approach of EE utilizing a paper point (PP) combined with a hollow fiber (HF) (abbreviated as PP-HF-EE) to extract various cationic and anionic model compounds from water samples. The study also explores, experimentally, the impact of agitation, organic filter composition, PP diameter, and PP brand on extraction performance, and proves that all these factors are quite important, especially when digital image analysis is utilized for determination. Furthermore, this work demonstrates the ease and feasibility of simultaneously extracting cations and anions using PP-HF-EE and proposes a straightforward method to enhance analyte concentration on the vertex of the PP through a base-to-vertex focusing. Lastly, it is demonstrated, using capillary electrophoresis coupled to a UV-Vis detector, that for PP-HF-EE, the extraction efficiency and pre-concentration factor are less dependent on other parameters when multiple PPs per sample are utilized, with signal enhancement values of up to 111 and 339 for nortriptyline and haloperidol, respectively. All the findings and strategies presented herein constitute significant contributions that can facilitate future research in method development, particularly in the utilization of PP-HF-EE and similar EE approaches.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Scaling Effects of the Weissenberg Number in Electrokinetic Oldroyd-B Fluid Flow Within a Microchannel. 微通道内电动奥尔德罗伊德-B 流体流动的魏森伯格数缩放效应
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-10-29 DOI: 10.1002/elps.202400175
Satwik Mukherjee, Sanjib Kr Pal, Partha P Gopmandal, Sankar Sarkar
{"title":"Scaling Effects of the Weissenberg Number in Electrokinetic Oldroyd-B Fluid Flow Within a Microchannel.","authors":"Satwik Mukherjee, Sanjib Kr Pal, Partha P Gopmandal, Sankar Sarkar","doi":"10.1002/elps.202400175","DOIUrl":"https://doi.org/10.1002/elps.202400175","url":null,"abstract":"&lt;p&gt;&lt;p&gt;This study attempts to extend previous research on electrokinetic turbulence (EKT) in Oldroyd-B fluid by investigating the relationship between the Weissenberg number ( &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;W&lt;/mi&gt; &lt;mi&gt;i&lt;/mi&gt;&lt;/mrow&gt; &lt;annotation&gt;$Wi$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) and the second-order velocity structure function ( &lt;math&gt; &lt;semantics&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt; &lt;mi&gt;v&lt;/mi&gt; &lt;mn&gt;2&lt;/mn&gt;&lt;/msubsup&gt; &lt;annotation&gt;$S_v^2$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; ) under applied electric fields. Inspired by Sasmal's demonstration in Sasmal (2022) of how heterogeneous zeta potentials induce turbulence above a critical &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;W&lt;/mi&gt; &lt;mi&gt;i&lt;/mi&gt;&lt;/mrow&gt; &lt;annotation&gt;$Wi$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , we develop a mathematical framework linking &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;W&lt;/mi&gt; &lt;mi&gt;i&lt;/mi&gt;&lt;/mrow&gt; &lt;annotation&gt;$Wi$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; to turbulent phenomena. Our analysis incorporates recent findings on AC (Zhao & Wang, 2017) and DC (Zhao & Wang 2019) EKT, which have defined scaling laws for velocity and scalar structure functions in the forced cascade region. Our finding shows that &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt; &lt;mi&gt;v&lt;/mi&gt; &lt;mn&gt;2&lt;/mn&gt;&lt;/msubsup&gt; &lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt; &lt;mi&gt;l&lt;/mi&gt; &lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt; &lt;mo&gt;∼&lt;/mo&gt; &lt;msubsup&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt; &lt;mrow&gt;&lt;mn&gt;4&lt;/mn&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;msup&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;mrow&gt;&lt;mn&gt;2&lt;/mn&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt; &lt;/msup&gt; &lt;/mrow&gt; &lt;annotation&gt;$S_v^2(l) sim lambda _1^{4/5} l^{2/5}$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; and &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt; &lt;mi&gt;σ&lt;/mi&gt; &lt;mn&gt;2&lt;/mn&gt;&lt;/msubsup&gt; &lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt; &lt;mi&gt;l&lt;/mi&gt; &lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt; &lt;mo&gt;∼&lt;/mo&gt; &lt;msubsup&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt; &lt;mrow&gt;&lt;mo&gt;-&lt;/mo&gt; &lt;mn&gt;2&lt;/mn&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;msup&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;mrow&gt;&lt;mn&gt;4&lt;/mn&gt; &lt;mo&gt;/&lt;/mo&gt; &lt;mn&gt;5&lt;/mn&gt;&lt;/mrow&gt; &lt;/msup&gt; &lt;/mrow&gt; &lt;annotation&gt;$S_sigma ^2(l) sim lambda _1^{-2/5} l^{4/5}$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , for a length scale &lt;math&gt;&lt;semantics&gt;&lt;mi&gt;l&lt;/mi&gt; &lt;annotation&gt;$l$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , and &lt;math&gt; &lt;semantics&gt;&lt;mrow&gt;&lt;mi&gt;W&lt;/mi&gt; &lt;mi&gt;i&lt;/mi&gt; &lt;mo&gt;=&lt;/mo&gt; &lt;mfrac&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt; &lt;msub&gt;&lt;mi&gt;u&lt;/mi&gt; &lt;mi&gt;l&lt;/mi&gt;&lt;/msub&gt; &lt;/mrow&gt; &lt;mi&gt;l&lt;/mi&gt;&lt;/mfrac&gt; &lt;/mrow&gt; &lt;annotation&gt;$Wi = frac{lambda _1 u_l}{l}$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; , where &lt;math&gt; &lt;semantics&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;u&lt;/mi&gt; &lt;mi&gt;l&lt;/mi&gt;&lt;/msub&gt; &lt;mo&gt;=&lt;/mo&gt; &lt;msqrt&gt; &lt;mrow&gt;&lt;msubsup&gt;&lt;mi&gt;S&lt;/mi&gt; &lt;mi&gt;u&lt;/mi&gt; &lt;mn&gt;2&lt;/mn&gt;&lt;/msubsup&gt; &lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt; &lt;mi&gt;l&lt;/mi&gt; &lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt; &lt;/mrow&gt; &lt;/msqrt&gt; &lt;/mrow&gt; &lt;annotation&gt;$u_l = sqrt {S_u^2(l)}$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; is a velocity fluctuations quantity and &lt;math&gt; &lt;semantics&gt;&lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt; &lt;annotation&gt;$lambda _1$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; denotes the time relaxation parameter. This work establishes a positive correlation between &lt;math&gt; &lt;semantics&gt;&lt;msub&gt;&lt;mi&gt;λ&lt;/mi&gt; &lt;mn&gt;1&lt;/mn&gt;&lt;/msub&gt; &lt;annotation&gt;$lambda _1$&lt;/annotation&gt;&lt;/semantics&gt; &lt;/math&gt; and turbulent flow phenomena through a rigorous analysis of velocity structure functions, thereby offe","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pressurized liquid extraction of sulfite for quantitation by capillary zone electrophoresis. 利用毛细管区带电泳对亚硫酸盐进行加压液体萃取定量。
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-10-29 DOI: 10.1002/elps.202400115
Samantha Gonçalves, Luciano Molognoni, Heitor Daguer, Ana Paula Fabichak Ribeiro, Carolina Turnes Pasini Deolindo, Luciano Vitali, Rodrigo Hoff
{"title":"Pressurized liquid extraction of sulfite for quantitation by capillary zone electrophoresis.","authors":"Samantha Gonçalves, Luciano Molognoni, Heitor Daguer, Ana Paula Fabichak Ribeiro, Carolina Turnes Pasini Deolindo, Luciano Vitali, Rodrigo Hoff","doi":"10.1002/elps.202400115","DOIUrl":"10.1002/elps.202400115","url":null,"abstract":"<p><p>Sulfite is an additive used in shrimp processing to prevent discoloration. However, sulfite can cause health issues for sensitive consumers, making its monitoring necessary. Determining sulfite concentrations is complex because the Monier-Williams reference method is laborious and has low analytical throughput. Additionally, new techniques are needed to stabilize sulfite during the extraction process because this analyte undergoes rapid oxidation. A new method involving extraction and derivatization of sulfite with formaldehyde through automated pressurized liquid extraction (PLE), followed by quantitation by capillary zone electrophoresis with diode-array detector in indirect mode, was developed and optimized using multivariate planning. The PLE procedure was compared to another solid-liquid extraction method. The new method successfully stabilized and extracted sulfite from shrimp in few steps with adequate precision (CV < 3.8%), producing extracts that were stable for 10 days. Recovery was satisfactory (97%-99%), and the limits of detection (4.6 mg kg<sup>-1</sup>) and quantitation (15.4 mg kg<sup>-1</sup>) were suitable for the intended purpose.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Special issue on “Foodomics and Advanced Food Analysis,” Autumn 2024 食品组学与高级食品分析 "特刊,2024 年秋。
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-10-29 DOI: 10.1002/elps.202400222
Alejandro Cifuentes, Miguel Herrero
{"title":"Special issue on “Foodomics and Advanced Food Analysis,” Autumn 2024","authors":"Alejandro Cifuentes,&nbsp;Miguel Herrero","doi":"10.1002/elps.202400222","DOIUrl":"10.1002/elps.202400222","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"46 3-4","pages":"133-134"},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Method Validation for the Determination of Ciprofloxacin in Lucilia sericata Larvae via Capillary Electrophoresis Combined With Mass Spectrometry 通过毛细管电泳结合质谱法测定蚕蛾幼虫体内环丙沙星的方法验证
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-10-25 DOI: 10.1002/elps.202400118
Magdalena Czuma-Pokusa, Maria Walczak
{"title":"Method Validation for the Determination of Ciprofloxacin in Lucilia sericata Larvae via Capillary Electrophoresis Combined With Mass Spectrometry","authors":"Magdalena Czuma-Pokusa,&nbsp;Maria Walczak","doi":"10.1002/elps.202400118","DOIUrl":"10.1002/elps.202400118","url":null,"abstract":"<div>\u0000 \u0000 <p>Substances derived from insects can serve therapeutic functions due to their diverse biological properties. This article focuses on the species <i>Lucilia sericata</i> and the benefits of larval therapy in patients who, due to hospitalization, have developed pressure ulcers and other difficult-to-heal wounds. Larval therapy, also known as maggot debridement therapy, employs sterile fly larvae to treat chronic, non-healing wounds by enzymatically degrading necrotic tissue and decreasing bacterial colonization. The larvae are applied to the wound for a period of 48–72 h, during which they effectively clean the wound and stimulate tissue regeneration. This therapeutic approach is particularly efficacious for recalcitrant wounds, such as diabetic foot ulcers and pressure sores, which have not responded to conventional treatments. Larvae may also constitute an alternative material in entomotoxicological studies to detect substances ingested at not only toxic but also therapeutic doses. The present work describes a method for assaying ciprofloxacin in <i>L. sericata</i> larvae using capillary electrophoresis coupled to mass spectrometry. The developed method features high sensitivity with a limit of quantification of 100 ± 0.018 ng/mL, as well as accuracy and precision estimated within 87%–103% and 1%–4%, respectively. An application of a simple and fast precipitation of proteins procedure for sample cleaning resulted in a highly satisfactory recovery of the analyte (90%–104%). The method was linear in a range of 100–1000 ng/mL with a determination coefficient higher than 0.9973. The method was used to determine ciprofloxacin in larval homogenate after antibiotic administration to the patient at a dose of 500 mg twice daily per os during application of the larvae dressing. Ciprofloxacin was shown to distribute from the patient's circulation to the larvae at a concentration of 150 ng/mL (750 ng/g).</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 21-22","pages":"1939-1945"},"PeriodicalIF":3.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel Applications of CE-ICP-MS/MS: Monitoring of Antiaging GHK–Cu Cosmetic Component Encapsulation in Liposomes CE-ICP-MS/MS 的新应用:监测脂质体中封装的抗衰老 GHK-Cu 化妆品成分。
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-10-25 DOI: 10.1002/elps.202400047
Joanna Zajda, Emilia Wadych, Karolina Ogórek, Marcin Drozd, Magdalena Matczuk
{"title":"Novel Applications of CE-ICP-MS/MS: Monitoring of Antiaging GHK–Cu Cosmetic Component Encapsulation in Liposomes","authors":"Joanna Zajda,&nbsp;Emilia Wadych,&nbsp;Karolina Ogórek,&nbsp;Marcin Drozd,&nbsp;Magdalena Matczuk","doi":"10.1002/elps.202400047","DOIUrl":"10.1002/elps.202400047","url":null,"abstract":"<div>\u0000 \u0000 <p>The hyphenation of the separation technique with the high-sensitive mass spectrometry detection is one of the driving forces of modern analysis enabling measurements in complex matrices. In particular, capillary electrophoresis coupled to inductively coupled plasma tandem mass spectrometry allows for speciation analysis of selected analytes with a superior resolution. The mild, physiological-friendly conditions of this separation technique offer the unique advantage of analyzing chemical entities in their intact form, which has been successfully exploited in various areas. Herein, we report the pioneering application of such a hyphenated technique in the cosmetic field to investigate the encapsulation of copper tripeptide complex (GHK–Cu) in liposomes. By monitoring copper and phosphorus signals, the formation of liposomes via a simple ethanol injection method was confirmed, and the concentration of GHK–Cu in the liposomes was assessed. The application of coupling of capillary electrophoresis with inductively coupled plasma tandem mass spectrometry (CE-ICP-MS/MS) in cosmetic studies could lead to the development of diverse liposomal formulations with preferential properties and expand their accessibility.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 21-22","pages":"1946-1954"},"PeriodicalIF":3.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial Board: Electrophoresis 19–20'24 编辑委员会:电泳 19-20'24
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-10-24 DOI: 10.1002/elps.202470102
{"title":"Editorial Board: Electrophoresis 19–20'24","authors":"","doi":"10.1002/elps.202470102","DOIUrl":"https://doi.org/10.1002/elps.202470102","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 19-20","pages":"1659"},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202470102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142555359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Contents: Electrophoresis 19–20'24 内容:电泳 19-20'24
IF 3 3区 生物学
ELECTROPHORESIS Pub Date : 2024-10-24 DOI: 10.1002/elps.202470103
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