Investigation of Fatty Acids in Biological Fluid Samples and Analysis by Capillary Electrophoresis: State of the Art and Applications.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-03-18 DOI:10.1002/elps.8134

Patrícia Abranches Geraldo, Maria Patrícia do Nascimento, Bruna Marchiori Berlande, Jéssica Cordeiro Queiroz de Souza, Luiz Henrique Cantarino Adriano, Marcone Augusto Leal de Oliveira

{"title":"Investigation of Fatty Acids in Biological Fluid Samples and Analysis by Capillary Electrophoresis: State of the Art and Applications.","authors":"Patrícia Abranches Geraldo, Maria Patrícia do Nascimento, Bruna Marchiori Berlande, Jéssica Cordeiro Queiroz de Souza, Luiz Henrique Cantarino Adriano, Marcone Augusto Leal de Oliveira","doi":"10.1002/elps.8134","DOIUrl":null,"url":null,"abstract":"<p><p>Fatty acids are vital to various physiological processes, making their analysis crucial for understanding metabolic, nutritional, and pathological conditions. Traditional methods for its analysis in biological samples, such as gas chromatography and high-performance liquid chromatography, often require complex sample preparation, including derivatization and extraction steps. Capillary electrophoresis has emerged as a promising alternative, offering simpler sample preparation, fast analysis times, and reduced consumption of solvents and reagents, which is in line with the principles of green chemistry. Despite its potential, capillary electrophoresis remains underutilized in fatty acid analysis in biological samples. In this regard, this review discusses the state of the art in capillary electrophoresis application for fatty acid analysis in biological samples, highlighting the simplified sample preparation protocols and the technique's advantages over others.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ELECTROPHORESIS","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/elps.8134","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Fatty acids are vital to various physiological processes, making their analysis crucial for understanding metabolic, nutritional, and pathological conditions. Traditional methods for its analysis in biological samples, such as gas chromatography and high-performance liquid chromatography, often require complex sample preparation, including derivatization and extraction steps. Capillary electrophoresis has emerged as a promising alternative, offering simpler sample preparation, fast analysis times, and reduced consumption of solvents and reagents, which is in line with the principles of green chemistry. Despite its potential, capillary electrophoresis remains underutilized in fatty acid analysis in biological samples. In this regard, this review discusses the state of the art in capillary electrophoresis application for fatty acid analysis in biological samples, highlighting the simplified sample preparation protocols and the technique's advantages over others.

查看原文本刊更多论文

生物流体样品中脂肪酸的研究及毛细管电泳分析：现状及应用。

脂肪酸对各种生理过程至关重要，因此对其分析对于理解代谢、营养和病理状况至关重要。传统的生物样品分析方法，如气相色谱法和高效液相色谱法，通常需要复杂的样品制备，包括衍生化和提取步骤。毛细管电泳作为一种很有前途的替代方法，具有样品制备简单、分析时间短、溶剂和试剂消耗少等优点，符合绿色化学的原则。尽管有潜力，毛细管电泳在生物样品的脂肪酸分析中仍未得到充分利用。在这方面，本文综述了毛细管电泳在生物样品中脂肪酸分析中的应用现状，强调了简化的样品制备方案和该技术相对于其他技术的优势。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.