Domestic animal endocrinology最新文献

{"title":"Granulosa cell function in domestic animals: A review on the in vitro effects of FSH, insulin and insulin-like growth factor 1","authors":"Leon J. Spicer ,&nbsp;Excel Rio S. Maylem ,&nbsp;Luis Fernando Schütz","doi":"10.1016/j.domaniend.2025.106919","DOIUrl":"10.1016/j.domaniend.2025.106919","url":null,"abstract":"<div><div>Ovarian granulosa cells produce a variety of biologically active compounds in addition to steroid hormones that include numerous families of growth factors, cytokines and adipokines. Many of these function as endocrine, paracrine and autocrine hormones to regulate ovarian activity. The goal of this review is to provide an update on the evidence in domestic animals on how FSH, insulin and IGF1 regulate the function of granulosa cells with a focus on ovarian steroidogenesis and cell proliferation with comparisons across six domestic animals: pigs, cattle, horses, water buffalo, goats and sheep. In most species, FSH was not a mitogenic stimulus to granulosa cells whereas insulin and IGF1 were stimulatory to cell proliferation in the species it was evaluated. FSH, insulin and IGF1 were all stimulatory to granulosa cell steroidogenesis in the species it was studied. More research is needed to evaluate the role of insulin in the regulation of cell proliferation and steroidogenesis in water buffalo and goats. The role of IGF1 in regulating granulosa cell function in horses also needs further study. Most granulosa-cell secreted factors have direct effects (either positive or negative) on FSH-, insulin- and IGF1-induced steroid production in ovarian cells, but how they all work together to create a cumulative effect to regulate fertility will require further research.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106919"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish oil a source of omega-3 fatty acids affects hypothalamus heat resistance genes expressions and fatty acid composition in heat-stressed chicks","authors":"Hatem M. El-Tahan ,&nbsp;Chun Ik Lim ,&nbsp;Ahmad R. Alhimaidi ,&nbsp;Aiman A. Ammari ,&nbsp;Sungbo Cho ,&nbsp;In Ho Kim ,&nbsp;Hossam M. El-Tahan","doi":"10.1016/j.domaniend.2025.106915","DOIUrl":"10.1016/j.domaniend.2025.106915","url":null,"abstract":"<div><div>This study investigated the effects of fish oil (FO) supplementation on the hypothalamus heat resistance gene expressions and fatty acid composition of chicks under acute high-temperature stress, for treating Cholestasis. A total of 48 chicks (Ross 308) at age of 14 days were acclimatized to corn oil or FO (n = 24 for each) by oral gavaging for 10 days, and then subjected to heat stress (35 ± 1 °C, HT) for 3 h or maintained at the normal temperature (26 ± 1 °C, NT) as grouped as NT and FO-NT control and HT and FO-HT (n = 12 for each).. The results showed that FO supplementation had no significant (P &gt; 0.05) effect on feed intake or body weight. The FO-HT group exhibited (<em>P</em> <em>&lt;</em> <em>0.05</em>) a lower rectal temperature, and plasma interleukin-6 (IL-6), triglyceride and corticosterone levels, in accompany with lower expressions of hypothalamic adenine nucleotide translocators (ANT) and uncoupling protein (UCP) but increased (<em>P</em> <em>&lt;</em> <em>0.05</em>) plasma superoxide dismutase activity and hypothalamic neuropeptide-Y (NPY) and heat shock protein-70 (HSP-70) expressions.. Additionally, the FO-HT group (P &lt; 0.05) demonstrated a higher unsaturated fatty acid/saturated fatty acid (UFA/SFA) ratio in the breast muscle. These findings suggest that FO supplementation can enhance the heat resistance of broiler chicks under acute heat stress and alter the fatty acid composition of their breast muscle. However, further studies are needed to determine whether desirable fatty acids can cross the blood-brain barrier and their implications for human health.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106915"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active immunization with a novel recombinant GnRH vaccine inhibits reproductive function in male goats","authors":"Moyan Di ,&nbsp;Xinbao Gong ,&nbsp;Yanyun Zhu ,&nbsp;Shibao Guo ,&nbsp;Zhihao Pan ,&nbsp;Mengxian Li ,&nbsp;Zhuoya Wu ,&nbsp;Wei Zhang ,&nbsp;Xuelan Liu ,&nbsp;Ya Liu ,&nbsp;Yunsheng Li ,&nbsp;Jian Li ,&nbsp;Fugui Fang","doi":"10.1016/j.domaniend.2024.106908","DOIUrl":"10.1016/j.domaniend.2024.106908","url":null,"abstract":"<div><div>Gonadotropin-releasing hormone (GnRH) vaccines have been widely used to effectively inhibit gonadal development and reproductive function. To improve the immunogenicity of GnRH, we developed and evaluated the effects of GnRH6-kisspeptin-CRM197 immunization on the reproductive function in male goats. Thirty 3-month-old male goats (n = 30) were randomly assigned to control, surgical, and immunized groups. The immunized group received a 2 mL injection of the GnRH6-kisspeptin-CRM197 with a booster administered four weeks later. The control group was administered a white oil adjuvant. Blood samples were collected at regular intervals, and at week 20, the animals were euthanized for tissue collection. Serum antibody titers and testosterone levels were measured using ELISA and CLIA, respectively. Testicular parameters and histology were evaluated. The mRNA levels of reproductive-related genes in the HPG axis were measured using RT-qPCR. The results showed that the immunized goats had significantly increased serum GnRH and kisspeptin antibodies (<em>P</em> &lt; 0.05) but decreased testosterone concentrations (<em>P</em> &lt; 0.05) compared to the control group. Testicular size and histology were significantly affected in the immunized group, with notable reductions in testicular weight and dimensions (<em>P</em> &lt; 0.01), and evidence of vacuolar degeneration and suppressed sperm production. The mRNA levels of <em>FSHβ</em> and <em>LHβ</em> in the pituitary, as well as <em>FSHR, LHR, 3βHSD</em>, and <em>17βHSD</em> in the testis, were significantly lower in the immunized group compared to controls (<em>P</em> &lt; 0.05). These findings suggest that GnRH6-kisspeptin-CRM197 is a safe antigen and a promising immunocastration vaccine with enhanced efficacy.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106908"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in serum concentrations of visfatin and betatrophin in dogs with diabetes mellitus","authors":"Alexandra Slon,&nbsp;Michal Mazaki-Tovi","doi":"10.1016/j.domaniend.2025.106914","DOIUrl":"10.1016/j.domaniend.2025.106914","url":null,"abstract":"<div><div>Canine diabetes mellitus (DM) is associated with hyperglycemia and hyperlipidemia. Visfatin and betatrophin are adipokines involved in the pathophysiology of insulin resistance and deranged lipid metabolism, and are also altered in obesity. We hypothesized that visfatin and betatrophin serum concentrations are altered in diabetic dogs, irrespective of their body condition. The study included 23 newly-diagnosed DM (NDDM) dogs, 34 insulin-treated DM (ITDM) dogs and 24 healthy dogs. Body condition score was determined and fasted serum samples were collected for measurement of betatrophin, visfatin and insulin serum concentrations. Visfatin concentrations (mean, 95% CI) were lower in overweight NDDM (4.5 ng/mL, 2.5-8.1, P=0.05) and ITDM (2.8 ng/mL 1.8-4.3, P=0.006) than healthy (7.7 ng/mL, 5.0-11.7) dogs, but were not different among lean dogs, and were negatively correlated to betahydroxybutyric acid in ITDM dogs (r=-0.59, P=0.05). Betatrophin concentrations were lower in NDDM (69 pg/mL, 43-112, P&lt;0.001) and ITDM (53 pg/mL, 34-84, P&lt;0.001) than healthy (267 pg/mL, 167-426) dogs. Among NDDM dogs, betatrophin concentrations were higher in those with concurrent liver disease (203 pg/mL, 49-844) than those with no evidence of liver disease (19 pg/mL, 4-90, P=0.007), and were negatively correlated with glucose concentrations (r=-0.44, P=0.04) and creatinine (r=-0.60, P=0.003). Insulin concentrations were not different among NDDM and healthy dogs. Among NDDM dogs, insulin concentrations were negatively correlated to concentrations of betahydroxybutyric acid (r=-0.65, P=0.002) and were lower in dogs with concurrent kidney disease (5.6 mU/L, 2.1-15.3) compared to those with no evidence of kidney disease (20.6 mU/L, 8.0-53.2, P=0.018). In conclusion, DM in dogs is associated with alteration in visfatin and betatrophin concentrations that are not resolved by insulin treatment.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106914"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum insulin-like growth factor 1 concentrations in non-diabetic cats with chronic kidney disease","authors":"J.P. Rey Amunategui ,&nbsp;E.M. Molina ,&nbsp;G.A. Pompili ,&nbsp;J. Mas ,&nbsp;O.P. Pignataro ,&nbsp;D.D. Miceli","doi":"10.1016/j.domaniend.2024.106898","DOIUrl":"10.1016/j.domaniend.2024.106898","url":null,"abstract":"<div><div>Feline hypersomatotropism (HST) can develop in both diabetic and non-diabetic cats, but studies evaluating the prevalence of HST in cats without diabetes mellitus (DM) are lacking. The aims of the study were to evaluate circulating insulin-like growth factor 1 (IGF-1) in non-diabetic cats with chronic kidney disease (CKD), to assess whether there is a correlation between general test of renal function and IGF-1 concentration in cats with CKD, and to screen this population for the presence of HST. In this prospective study, one hundred fifty-four non-diabetic cats (<em>n</em> = 154) with CKD from referral centers in Buenos Aires (Argentina) were evaluated. Serum IGF-1 concentration was measured as part of the routine tests for CKD and compared with a healthy control group of 50 cats without CKD. The median serum IGF-1 concentration in the total population of cats with CKD was 500 ng/mL (range 34–1593 ng/mL). Median serum IGF-1 concentrations of cats with IRIS stage 1 (<em>n</em> = 13), stage 2 (<em>n</em> = 86), stage 3 (<em>n</em> = 40) and stage 4 (<em>n</em> = 15) of CKD were 230 ng/mL (range 58–951 ng/mL), 473 ng/mL (range 34–1456 ng/mL), 597 ng/mL (range 123–1593 ng/mL), 569 ng/mL (range 123–1045 ng/mL), respectively. None of the cats in the control group had IGF-1 concentration &gt;1000 ng/mL (median 505 ng/mL, range 114–720 ng/mL). There was a positive linear correlation between serum IGF-1 and creatinine concentrations in cats with CKD (r= 0.22, 95% confidence interval 0.06–0.37 <em>P</em>=0.005). A proportion of 5.8% (95% confidence interval 2.7-10.8%) of non-diabetic cats with CKD had markedly increased IGF-1 concentrations (cut-off IGF-1 &gt;1000 ng/mL). Pituitary enlargement was detected on computed tomography in 3/4 of these cases. Eighteen cats (11.6%) had serum IGF-1 concentrations in the “grey zone” between 800 and 1000 ng/mL. A small proportion of non-diabetic cats with CKD had an IGF-1 concentration in a range that is consistent with HST in diabetic cats. Likewise, the progression of CKD in cats without DM correlates with increases in serum IGF-1 concentrations.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106898"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of Anti-Müllerian hormone serum concentration measured in proestrus and estrus with the litter size as a fertility marker in bitches","authors":"Theresa Hornberger ,&nbsp;Sven Reese ,&nbsp;Klaus Perbandt ,&nbsp;Andrea Meyer-Lindenberg ,&nbsp;Beate Walter","doi":"10.1016/j.domaniend.2024.106897","DOIUrl":"10.1016/j.domaniend.2024.106897","url":null,"abstract":"<div><div>The use of the anti-Müllerian hormone (AMH) serum concentration as a fertility marker has been shown in cows, sheep and mares and has been indicated in one study in female dogs. The aim of this study was to investigate the connection between the AMH serum concentration, taken at two defined time points during the bitch`s heat, and the litter size to investigate whether AMH can be used as a practical measurement for the individual breeding bitch to predict litter size. The study was carried out on 27 healthy female dogs presented for pre-breeding examination, considering all previously known influencing factors on AMH in the bitch such as age, body weight and estrous cycle phase at the time the sample was taken. Due to the AMH increase in early proestrus and its drop around ovulation, AMH was measured in blood samples taken within the first three days of heat (AMH1) and near ovulation (AMH2) with AMH1 being significantly higher than AMH 2 (p &lt; 0.001). There was a highly significant negative correlation of body weight and AMH at both sampling times (p &lt; 0.001). There were no significant results when the dogs were paired and grouped according to high or low AMH concentrations, but a significant effect of AMH serum concentration on litter size was found in the multifactorial analysis when the dogs were matched according to their body weight (AMH1: p = 0.022; AMH2: p = 0.030). In conclusion, a significant effect of the AMH concentration and the litter size among female dogs with matching body weight could be found, but a much larger sample collection is needed to evaluate reference intervals for AMH for bitches of different weight to predict the fertility of an individual bitch in the future.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106897"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadotropins, local factors and a variety of adipokines contribute to regulate swine granulosa cell function","authors":"G. Basini,&nbsp;F. Grasselli","doi":"10.1016/j.domaniend.2025.106918","DOIUrl":"10.1016/j.domaniend.2025.106918","url":null,"abstract":"<div><div>This paper reviews the role of endocrine regulators in swine ovarian cell functions, highlighting the intricate hormonal interactions that drive reproductive and metabolic processes. The pig represents a valuable model for human biology due to physiological and anatomical similarities. Understanding the endocrine mechanisms in swine can provide insights about human reproductive health and metabolic disorders. The present review describes some key hormones involved, including gonadotropins, sex steroids, and adipokines, and their impacts on ovarian folliculogenesis and steroidogenesis. Emphasis is placed on the crosstalk between the ovary and adipose tissue, which is critical for maintaining reproductive and metabolic homeostasis.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106918"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pregnancy-induced mechanisms regulating central and peripheral leptin sensitivity: lessons from sheep","authors":"Malgorzata Szczesna,&nbsp;Katarzyna Kirsz,&nbsp;Dorota A. Zieba","doi":"10.1016/j.domaniend.2024.106910","DOIUrl":"10.1016/j.domaniend.2024.106910","url":null,"abstract":"<div><div>This review describes various aspects of the leptin resistance phenomenon and related physiological mechanisms that occur in pregnant sheep. Its main aim is to analyze the mechanisms that determine the occurrence of pregnancy-induced leptin resistance and to investigate the accompanying processes that affect the physiology of pregnancy and lactation in livestock. The main purpose of this analysis was to comprehensively understand the phenomenon of leptin resistance, including the causes of its emergence and its effects on nonrodent organisms.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106910"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From spermatogenesis to fertilisation: the role of melatonin on ram spermatozoa","authors":"Adriana Casao,&nbsp;Victoria Peña-Delgado,&nbsp;Rosaura Pérez-Pe","doi":"10.1016/j.domaniend.2025.106916","DOIUrl":"10.1016/j.domaniend.2025.106916","url":null,"abstract":"<div><div>This review presents recent findings on the effect of melatonin on ram spermatozoa. This hormone regulates seasonal reproduction in the ovine species through the hypothalamic-pituitary-gonadal axis, but it also exerts direct effects on spermatogenesis, seminal quality and fertility. In the testis, melatonin stimulates blood flow to this organ, but it also appears to be involved in the differentiation of spermatogonial stem cells and the secretion of testosterone through the MT1 and MT2 receptors. In the epididymis, this hormone modulates sperm maturation and the secretory activity of epidydimal epithelial cells. In addition, the antioxidant activity of melatonin may protect spermatozoa from oxidative damage during their formation in the testis and their maturation in the epididymis. After ejaculation, the melatonin present in seminal plasma may also protect sperm from oxidative damage and premature capacitation and may improve seminal quality. Finally, once the sperm begins its transit through the female genital tract, melatonin may modulate sperm capacitation. Thus, melatonin could have a bimodal activity in ram sperm capacitation, so high concentrations, such as those in seminal plasma, have a decapacitating effect. In contrast, low concentrations, such as those present in the female reproductive tract, may promote it, likely through interaction with MT2 receptors. In addition, melatonin could also be involved in chemotaxis and fertilisation, although further studies are needed to elucidate the specific role of melatonin in these processes. Finally, the effect of latitude and melatonin receptor gene polymorphisms in ram reproduction is also discussed.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106916"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utero-placental adaptations in response to intrauterine growth restriction in swine","authors":"Morgan S. Clemens ,&nbsp;Maria F. Tyree ,&nbsp;Claire Stenhouse","doi":"10.1016/j.domaniend.2025.106917","DOIUrl":"10.1016/j.domaniend.2025.106917","url":null,"abstract":"<div><div>Intrauterine growth restriction (IUGR) is a common condition in swine associated with high piglet mortality and morbidity that develops in early gestation. This review article explores differences in uterine and placental tissues associated with IUGR fetuses compared to their normally-grown littermates at different stages of gestation. Specifically, we will review the available knowledge to date describing differences in 1) structure, 2) cellular apoptosis and proliferation, 3) adhesion, and 4) angiogenesis in endometrial and placental tissues associated with IUGR fetuses across gestation. Improved understanding of the mechanisms regulating IUGR is essential for the development of strategies to minimize the impact of IUGR in swine operations, thus improving reproductive efficiency and animal welfare.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106917"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}