Domestic animal endocrinology最新文献

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Association between post-ACTH cortisol and trilostane dosage in dogs with pituitary-dependent hypercortisolism 垂体依赖性皮质醇过多症犬的 ACTH 后皮质醇与曲托司坦用量之间的关系
IF 1.9 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-07-04 DOI: 10.1016/j.domaniend.2024.106871
Fernanda Nastri Gouvêa , Alessandra Martins Vargas , Ednaldo Carvalho Guimarães , Leandro Zuccolotto Crivellenti , Caio Santos Pennacchi , Hévila Dutra Barbosa de Cerqueira , Luana de Oliveira Branco , Natani Silva Reis , Sofia Borin-Crivellenti
{"title":"Association between post-ACTH cortisol and trilostane dosage in dogs with pituitary-dependent hypercortisolism","authors":"Fernanda Nastri Gouvêa ,&nbsp;Alessandra Martins Vargas ,&nbsp;Ednaldo Carvalho Guimarães ,&nbsp;Leandro Zuccolotto Crivellenti ,&nbsp;Caio Santos Pennacchi ,&nbsp;Hévila Dutra Barbosa de Cerqueira ,&nbsp;Luana de Oliveira Branco ,&nbsp;Natani Silva Reis ,&nbsp;Sofia Borin-Crivellenti","doi":"10.1016/j.domaniend.2024.106871","DOIUrl":"10.1016/j.domaniend.2024.106871","url":null,"abstract":"<div><p>Trilostane is the current treatment of choice for managing pituitary-dependent hypercortisolism (PDH) in dogs. While prescribing higher initial doses may elevate the risk of iatrogenic hypocortisolism, opting for more conservative approach could result in delayed disease control, since most individuals end up requiring dosage increases. The adrenocorticotrophin stimulation test (ACTHst), a widely recognized hormonal test for assessing adrenal function, is an essential tool for monitoring the pharmacological treatment of canine hypercortisolism (CH) that can also be used for diagnostic purposes. The aim of this study was to investigate the relationship between post-ACTH cortisol (cpACTH) at PDH diagnosis and the required trilostane dose for sign control and endogenous cortisol regulation in dogs, considering a hypothesis that higher serum cpACTH concentration would necessitate a higher trilostane dosage for disease management. Data for 43 dogs with PDH had their diagnostic cpACTH recorded and correlated to the trilostane dosage necessary to control clinical signs and achieve satisfactory cortisol levels (ideally 2-7 μg/dL). The odds ratio (p=0.042) suggests that dogs with cpACTH ≥ 27 μg/dL at diagnosis are 96% more likely to need a higher trilostane dosage for achieving satisfactory control of PDH. Thus, cpACTH was found to be associated with the final trilostane dose for controlling PDH in dogs.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106871"},"PeriodicalIF":1.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141710070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modulation of PI3K/AKT/mTOR signaling pathway in the ovine liver and duodenum during early pregnancy 妊娠早期绵羊肝脏和十二指肠中 PI3K/AKT/mTOR 信号通路的调节
IF 1.9 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-06-27 DOI: 10.1016/j.domaniend.2024.106870
Hongxu Fang , Xinxin Wang , Zhongyue Wang , Xiaoxin Ma, Leying Zhang, Ling Yang
{"title":"Modulation of PI3K/AKT/mTOR signaling pathway in the ovine liver and duodenum during early pregnancy","authors":"Hongxu Fang ,&nbsp;Xinxin Wang ,&nbsp;Zhongyue Wang ,&nbsp;Xiaoxin Ma,&nbsp;Leying Zhang,&nbsp;Ling Yang","doi":"10.1016/j.domaniend.2024.106870","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106870","url":null,"abstract":"<div><p>The liver and intestine play a critical role in nutrient absorption, storage, and metabolism. The aim of this study was to evaluate expression pattern of phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of the rapamycin (mTOR) signaling pathway that included PI3K, AKT1, mTOR, FoxO1, SREBP-1, PPARα, PTEN and FXR in the maternal liver and duodenum. Ovine livers and duodenums were sampled at day 16 of the estrous cycle, and at days 13, 16 and 25 of gestation, and RT-qPCR, western blot and immunohistochemistry analysis were used to detect mRNA and protein expression. The results showed that expression of PI3K, AKT1, p-mTOR, FoxO1, SREBP-1 and PTEN upregulated in the maternal liver, and PPARα upregulated in the duodenum. However, expression of FoxO1, SREBP-1 and PTEN in the duodenum downregulated during early pregnancy. In addition, expression levels of SREBP-1, PTEN and PPARα in the maternal liver, and PI3K in the duodenum peaked at day 13 of pregnancy. In addition, expression levels of PI3K, p-mTOR and FoxO1 in the liver, and AKT1 and p-mTOR in the duodenum peaked at day 16 of pregnancy. Nevertheless, expression levels of FXR both in the maternal liver duodenum downregulated at days 13 and 16 of pregnancy. In conclusion, early pregnancy regulated expression pattern of PI3K/AKT/mTOR signaling pathway in the ovine liver and duodenum in a pregnancy stage-specific and tissue-specific manner, which may be necessary for the adaptations in maternal hepatic nutrient metabolism and intestinal nutrient absorption early pregnancy.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106870"},"PeriodicalIF":1.9,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chronic inflammation decreases arcuate kisspeptin expression in male sheep 慢性炎症会降低雄性绵羊弓状吻肽的表达。
IF 2.1 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-06-09 DOI: 10.1016/j.domaniend.2024.106868
AN Renwick , BK Whitlock , CC Nestor , JA Daniel , L Strickland , AS Lear , M Adkins , C Griffin , A Esteller-Vico
{"title":"Chronic inflammation decreases arcuate kisspeptin expression in male sheep","authors":"AN Renwick ,&nbsp;BK Whitlock ,&nbsp;CC Nestor ,&nbsp;JA Daniel ,&nbsp;L Strickland ,&nbsp;AS Lear ,&nbsp;M Adkins ,&nbsp;C Griffin ,&nbsp;A Esteller-Vico","doi":"10.1016/j.domaniend.2024.106868","DOIUrl":"10.1016/j.domaniend.2024.106868","url":null,"abstract":"<div><p>Lipopolysaccharide (LPS) from Gram-negative bacteria induces an immune response and impairs reproduction through suppression of gonadotropin releasing hormone (GnRH), subsequently luteinizing hormone (LH) secretion. While there is evidence that acute inflammation inhibits kisspeptin, little is known about the impact of chronic inflammation on this key reproductive neuropeptide in livestock species. Thus, we sought to examine a central mechanism whereby LPS suppresses LH secretion in sheep. Twenty wethers were randomly assigned to one of five treatment groups: control (CON; n=4), single acute IV LPS dose (SAD; n=4), daily acute IV LPS dose (DAD; n=4), daily increasing IV LPS dose (DID; n=4), and chronic subcutaneous LPS dose (CSD; n=4). On Days 1 and 7, blood samples were collected every 12 minutes for 360 minutes using jugular venipuncture. Following blood collection on Day 7, all animals were euthanized, brain tissue was perfused with 4% paraformaldehyde, and hypothalamic blocks were removed and processed for immunohistochemistry. On Day 1, LH pulse frequency was significantly lower (p=0.02) in SAD (0.25 ± 0.1 pulses/hour), DAD (0.25 ± 0.1 pulses/hour), DID (0.35 ± 0.1 pulses/hour), and CSD (0.40 ± 0.1 pulses/hour) compared to CON (0.70 ±0.1 pulses/hour). On Day 7, only DID animals (0.35 ± 0.1 pulses/hour) had significantly lower (p=0.049) LH pulse frequency compared to controls (0.85 ± 0.1 pulse/hour). Furthermore, only DID animals (33.3 ± 10.9 cells/section/animal) had significantly fewer (p=0.001) kisspeptin-immunopositive cells compared to controls (82.6 ± 13.6 cells/section/animal). Taken together, we suggest that daily increasing doses of LPS is a powerful inhibitor of kisspeptin neurons in young male sheep and a physiologically relevant model to examine the impact of chronic inflammation on the reproductive axis in livestock.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106868"},"PeriodicalIF":2.1,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141414968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Incretin therapy in feline diabetes mellitus – A review of the current state of research 猫科动物糖尿病的胰岛素疗法--研究现状综述
IF 2.1 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-06-07 DOI: 10.1016/j.domaniend.2024.106869
Nina Haller , Thomas A. Lutz
{"title":"Incretin therapy in feline diabetes mellitus – A review of the current state of research","authors":"Nina Haller ,&nbsp;Thomas A. Lutz","doi":"10.1016/j.domaniend.2024.106869","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106869","url":null,"abstract":"<div><p>Incretin hormones potentiate the glucose-induced insulin secretion following enteral nutrient intake. The best characterised incretin hormones are glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) which are produced in and secreted from the gut in response to nutrient ingestion. The property of incretins to enhance endogenous insulin secretion only at elevated blood glucose levels makes them interesting therapeutics for type 2 diabetes mellitus with a better safety profile than exogenous insulin. While incretin therapeutics (especially GLP-1 agonists, and more recently also GLP-1 / GIP dual agonists and other drugs that influence the incretin metabolism (e.g., dipeptidyl peptidase-4 (DPP-4) inhibitors)) are already widely used treatment options for human type 2 diabetes, these drugs are not yet approved for the therapy of feline diabetes mellitus. This review provides an introduction to incretins and feline diabetes mellitus in general and summarises the current study situation on incretins as therapeutics for feline diabetes mellitus to assess their possible future potential in feline medicine. Studies to date on the use of GLP-1 receptor agonists (GLP-1RA) in healthy cats largely confirm their insulinotropic effect known from other species. In diabetic cats, GLP-1RAs appear to significantly reduce glycaemic variability (GV, an indicator for the quality of glycaemic control), which is important for the management of the disease and prevention of long-term complications. However, for widespread use in feline diabetes mellitus, further studies are required that include larger numbers of diabetic cats, and that consider and test a possible need for dose adjustments to overweight and diabetic cats. Also evaluation of the outcome of GLP-1RA monotherapy will be neceessary.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106869"},"PeriodicalIF":2.1,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141313796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Obesity and gestation in Criollo mares: endocrine and metabolic profiles Criollo 母马的肥胖和妊娠:内分泌和代谢特征。
IF 2.1 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-05-28 DOI: 10.1016/j.domaniend.2024.106857
Mateo Pardié , Irene Kalpokas , Francesca Freccero , Carolina Castagnetti , Ana Meikle
{"title":"Obesity and gestation in Criollo mares: endocrine and metabolic profiles","authors":"Mateo Pardié ,&nbsp;Irene Kalpokas ,&nbsp;Francesca Freccero ,&nbsp;Carolina Castagnetti ,&nbsp;Ana Meikle","doi":"10.1016/j.domaniend.2024.106857","DOIUrl":"10.1016/j.domaniend.2024.106857","url":null,"abstract":"<div><p>The aim of this study was to determine the effect of body condition score (BCS) on metabolic and endocrine parameters in pregnant Criollo mares (<em>n</em>=41), which were categorized according to their BCS as obese (7 to 9 BCS, <em>n</em>=26) or normal (5 to 7, <em>n</em>=15). Blood samples were taken during gestation in 3 periods: between 3.5 and 5 months (I), 8 and 9 months (II) and in the last month of gestation (III). The data was analyzed in the statistical model by mixed procedures, including BCS, gestational period and their interaction as fixed effects. BCS was only different in period I, as normal mares increased their BCS in the later periods. Leptin concentrations were greater in obese mares when compared to non-obese mares during all sampling periods (<em>P</em>&lt;0.01), while glucose concentrations were also greater in the former group (<em>P</em>&lt;0.01) but only during the first sampling period. Insulin concentrations and homeostatic model assessment for insulin resistance (HOMA-IR) index were greater also in obese mares in periods I and III (<em>P</em>&lt;0.05). Adiponectin concentrations in period I were lower in obese mares (<em>P</em>&lt;0.05). Cholesterol concentrations increased during gestation, and obese mares tended to have greater concentrations than nonobese mares (<em>P</em>&lt;0.1). Triglyceride concentrations were not affected by group or gestational period. This study revealed adaptations in carbohydrate and lipid metabolism during gestation in mares. Several parameters are dependent on the degree of body fat reserves, which are reflected in the concentrations of biomarkers such as leptin and adiponectin. Insulin concentration in obese mares was higher than non-obese mares at the end of gestation, a similar profile was observed for HOMA-IR although cutoff values are yet still to be validated.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106857"},"PeriodicalIF":2.1,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GATA4: Regulation of expression and functions in goat granulosa cells GATA4:山羊颗粒细胞中的表达和功能调控
IF 2.1 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-05-23 DOI: 10.1016/j.domaniend.2024.106859
Kexin Gao , Yeda Chen , Peijie Wang , Wenlin Chang , Binyun Cao , Liqiong Luo
{"title":"GATA4: Regulation of expression and functions in goat granulosa cells","authors":"Kexin Gao ,&nbsp;Yeda Chen ,&nbsp;Peijie Wang ,&nbsp;Wenlin Chang ,&nbsp;Binyun Cao ,&nbsp;Liqiong Luo","doi":"10.1016/j.domaniend.2024.106859","DOIUrl":"10.1016/j.domaniend.2024.106859","url":null,"abstract":"<div><p>GATA4 plays a pivotal role in the reproductive processes of mammals. However, the research on GATA4 in goat ovary is limited. This study aimed to study the expression and function of GATA4 in goat ovary. Utilizing real-time PCR and western blot analysis, we studied the expression and regulatory mechanisms of GATA4 in goat ovary and granulosa cells (GCs). We found that GATA4 was expressed in all follicle types in the goat ovary, with significantly higher levels in GCs of larger follicles (&gt;3 mm) compared to those in smaller follicles (&lt;3 mm). Additionally, we demonstrated that human chorionic gonadotrophin (hCG) induced <em>GATA4</em> mRNA expression via the activation of PKA, MEK, p38 MAPK, PKC, and PI3K pathways <em>in vitro</em>. Our study also showed that hCG suppressed the levels of miR-200b and miR-429, which in turn directly target GATA4, thereby modulating the basal and hCG-induced expression of GATA4. Functionally, we examined the effect of siRNA-mediated <em>GATA4</em> knockdown on cell proliferation and hormone secretion in goat GCs. Our results revealed that knockdown of <em>GATA4</em>, miR-200b, and miR-429 suppressed cell proliferation. Moreover, knockdown of <em>GATA4</em> decreased estradiol and progesterone production by inhibiting the promoter activities of <em>CYP11A1, CYP19A1, HSD3B,</em> and <em>StAR</em>. Collectively, our findings suggest a critical involvement of GATA4 in regulating goat GC survival and steroidogenesis.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106859"},"PeriodicalIF":2.1,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141130677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased insulin-like growth factor 1 concentrations in a population of non-diabetic cats with overweight/obesity 超重/肥胖的非糖尿病猫体内胰岛素样生长因子 1 浓度升高
IF 2.1 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-05-19 DOI: 10.1016/j.domaniend.2024.106858
D.D. Miceli , A. Jaliquias , M.F. Gallelli , J.D. García , C. Vecino , J.P. Rey Amunategui , G.A. Pompili , I.M. Espiñeira , J. Más , O.P. Pignataro
{"title":"Increased insulin-like growth factor 1 concentrations in a population of non-diabetic cats with overweight/obesity","authors":"D.D. Miceli ,&nbsp;A. Jaliquias ,&nbsp;M.F. Gallelli ,&nbsp;J.D. García ,&nbsp;C. Vecino ,&nbsp;J.P. Rey Amunategui ,&nbsp;G.A. Pompili ,&nbsp;I.M. Espiñeira ,&nbsp;J. Más ,&nbsp;O.P. Pignataro","doi":"10.1016/j.domaniend.2024.106858","DOIUrl":"10.1016/j.domaniend.2024.106858","url":null,"abstract":"<div><p>Feline hypersomatotropism (HST) is typically associated with diabetes mellitus (DM), whereas HST without concurrent DM has only been reported in a few cases. Weight gain may be observed in cats with HST. The aims of this study were to evaluate circulating insulin-like growth factor-1 (IGF-1) in non-diabetic cats with overweight/obesity, to screen this population for the presence of HST, and to assess whether there is a correlation between body weight/body condition score (BCS) and serum IGF-1 concentration in overweight/obese cats. In this prospective study, 80 overweight/obese cats from referral centers in Buenos Aires (Argentina) were evaluated. Serum IGF-1 was measured as part of the routine tests for overweight/obesity. Non-diabetic cats were included in the study if they had a BCS&gt;6/9. Twenty-nine cats were classified as overweight (BCS 7/9), whereas 51 were classified as obese (BCS 8-9/9). Median serum IGF-1 concentrations of cats with BCS 7/9, 8/9, and 9/9 were 570 ng/ml (range 123-1456 ng/ml), 634 ng/ml (range 151-1500 ng/ml), and 598 ng/ml (range 284-2450 ng/ml), respectively. There was a positive linear correlation between serum IGF-1 concentrations and body weight (r= 0.24, 95% CI 0.01-0.44 P=0.03), and between IGF-1 and BCS (r= 0.27, 95% CI 0.08-0.44 P=0.004). In total, 8.75% (95% confidence interval 3.6-17.2%) of the cats with overweight/obesity had IGF-1 concentrations &gt;1000 ng/ml. Pituitary enlargement was detected on computed tomography in 4/7 cases. These seven cats showed varying degrees of phenotypic changes consistent with acromegaly. A proportion of 8.75 % of overweight/obese non-diabetic cats from referral centers in Buenos Aires had serum IGF-1 concentration in a range consistent with HST in diabetic cats. Likewise, 5% of overweight/obese cats were likely to be diagnosed with HST, supported by evidence of pituitary enlargement. Serum IGF-1 concentrations were positively correlated with body weight and BCS in this population of cats. This study highlights the relevance of screening different populations of non-diabetic cats to increase the detection of HST/acromegaly.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106858"},"PeriodicalIF":2.1,"publicationDate":"2024-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141138798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Age-specific analysis of anti-müllerian hormone and liver biochemical parameters in freemartin holstein calves and heifers: A pilot study 自由马丁荷斯坦犊牛和小母牛抗苗勒管激素和肝脏生化参数的年龄特异性分析:一项试点研究
IF 2.1 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-05-09 DOI: 10.1016/j.domaniend.2024.106855
Gokhan Bozkurt , Feyyaz Kaya , Mehmet Yildiz , Sultan Sengul , Ismail Ilker Kocaer , Halil Erkan Sarıbas , Orsan Gungor , Ibrahim Tasal , Yigit Can Aslan , Serdar Dursun Ak , Afsin Koker
{"title":"Age-specific analysis of anti-müllerian hormone and liver biochemical parameters in freemartin holstein calves and heifers: A pilot study","authors":"Gokhan Bozkurt ,&nbsp;Feyyaz Kaya ,&nbsp;Mehmet Yildiz ,&nbsp;Sultan Sengul ,&nbsp;Ismail Ilker Kocaer ,&nbsp;Halil Erkan Sarıbas ,&nbsp;Orsan Gungor ,&nbsp;Ibrahim Tasal ,&nbsp;Yigit Can Aslan ,&nbsp;Serdar Dursun Ak ,&nbsp;Afsin Koker","doi":"10.1016/j.domaniend.2024.106855","DOIUrl":"10.1016/j.domaniend.2024.106855","url":null,"abstract":"<div><p>Freemartinism is the most common congenital anomaly among sexual disorders in dairy cows. This syndrome typically occurs in different-sex twin pregnancies and causes vascular anastomoses to form with the placenta in the early stages of fetal development. The study aims to determine the effectiveness of Anti-müllerian hormone (AMH) levels in calves and heifers of different age groups for diagnostic factors and to investigate the potential consequences of different hormone levels in different age groups on some liver biochemical parameters. The study involved 50 cattle from diverse age categories, divided into the freemartin group (FM Group, n=25) and the control group (C Group, n=25). Both FM and control groups were further divided into early-age (3-5 months), middle-aged (5-9 months), and older-aged groups (9-12 months). Serum AMH levels, along with total protein, albumin, and total cholesterol levels, were measured. While no statistically significant difference in AMH levels was observed in the early-age group (P:0.53), significant differences were determined in the middle (P:0.015) and older-age groups (P:0.01), where the FM group exhibited significantly decreased AMH levels compared to the control group. The evaluation of liver biochemistry revealed a statistically significant difference in total protein levels between the FM and control groups in the older age group (P:0.033). Consequently, it is reasonable to suggest that AMH levels may serve as a valid parameter for diagnosing freemartin syndrome in calves aged older than five months. Conversely, particularly in young calves, no significant differences in liver functionality were observed between freemartin-affected and healthy calves.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106855"},"PeriodicalIF":2.1,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141039973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The basic functions of rabbit ovarian granulosa cell are regulated by adipokines monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1 单核细胞趋化蛋白-1和纤溶酶原激活物抑制剂-1调节兔卵巢颗粒细胞的基本功能
IF 2.1 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-05-09 DOI: 10.1016/j.domaniend.2024.106856
Alexander V. Sirotkin , Zuzana Fabová , Barbora Loncová , Abdel Halim Harrath
{"title":"The basic functions of rabbit ovarian granulosa cell are regulated by adipokines monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1","authors":"Alexander V. Sirotkin ,&nbsp;Zuzana Fabová ,&nbsp;Barbora Loncová ,&nbsp;Abdel Halim Harrath","doi":"10.1016/j.domaniend.2024.106856","DOIUrl":"10.1016/j.domaniend.2024.106856","url":null,"abstract":"<div><p>The aim of the present study was to examine the influence of monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1) on ovarian cell functions. Rabbit ovarian granulosa cells were cultured with or without MCP-1 or PAI-1 (at 0, 0.1, 1, or 10 ng/ml). Cell viability, proliferation, cytoplasmic apoptosis and release of progesterone and estradiol were measured by Cell Counting Kit-8 (CCK-8), BrdU incorporation, and cell death detection assays and ELISA. The addition of either MCP-1 or PAI-1 increased cell viability and proliferation and decreased apoptosis. MCP-1 promoted, while PAI-1 suppressed, progesterone release. Both MCP-1 and PAI-1 reduced estradiol output. The present results suggest that MCP-1 or PAI-1 can be physiological promoters of rabbit ovarian cell viability and proliferation, inhibitors of apoptosis and regulators of ovarian steroidogenesis.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106856"},"PeriodicalIF":2.1,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141036737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prostaglandin F2α treatment does not hasten ovulation in weaned sows 前列腺素 F2α 治疗不会加速断奶母猪的排卵
IF 2.1 2区 农林科学
Domestic animal endocrinology Pub Date : 2024-05-07 DOI: 10.1016/j.domaniend.2024.106854
D.P. Fagundes , M.S. Lucca , B.G. Gasperin , D. Missio , M. Quirino , A.P.G. Mellagi , P.B.D. Gonçalves , F.P. Bortolozzo , R.R. Ulguim
{"title":"Prostaglandin F2α treatment does not hasten ovulation in weaned sows","authors":"D.P. Fagundes ,&nbsp;M.S. Lucca ,&nbsp;B.G. Gasperin ,&nbsp;D. Missio ,&nbsp;M. Quirino ,&nbsp;A.P.G. Mellagi ,&nbsp;P.B.D. Gonçalves ,&nbsp;F.P. Bortolozzo ,&nbsp;R.R. Ulguim","doi":"10.1016/j.domaniend.2024.106854","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106854","url":null,"abstract":"<div><p>This study evaluated the efficiency of prostaglandin F2α (PGF) to hasten ovulation in weaned sows. In experiment I, weaned sows detected in estrus (0 h) received: no hormone (Control; <em>n</em> = 56); 0.5 mg PGF IM at 0 h and 2 h (PGF0; <em>n</em> = 56); or 0.5 mg PGF IM at 24 h and 26 h (PGF24; <em>n</em> = 55). In experiment II, weaned sows that did not express estrus signs until 72 h after weaning (0 h) were assigned to: no hormone (Control; <em>n</em> = 45); 10 µg buserelin acetate IM at 0 h (Buserelin; <em>n</em> = 43); 0.5 mg PGF IM at 34 h and 36 h (PGF; <em>n</em> = 44); or 10 µg buserelin acetate IM at 0 h plus 0.5 mg PGF IM at 34 h and 36 h (Buserelin + PGF; <em>n</em> = 45). In experiment I, no effect of PGF on the interval treatment onset to ovulation was observed (<em>P</em> &gt; 0.05), and no treatment effect was observed on the relative or cumulative proportion of females that ovulated post-treatment onset (<em>P</em> &gt; 0.05). In experiment II, treatment onset to ovulation interval was shorter for Buserelin group than for PGF group (<em>P</em> &lt; 0.05), and a higher cumulative percentage of Buserelin treated sows ovulated up to 48 h compared to PGF and Control groups (<em>P</em> &lt; 0.01), with no differences from Buserelin + PGF. Treatments did not affect total number of piglets born in both experiments (<em>P</em> &gt; 0.05). In conclusion, PGF did not hasten ovulation timing or affect litter size in weaned sows.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106854"},"PeriodicalIF":2.1,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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