Domestic animal endocrinology最新文献

{"title":"Age-specific analysis of anti-müllerian hormone and liver biochemical parameters in freemartin holstein calves and heifers: A pilot study","authors":"Gokhan Bozkurt ,&nbsp;Feyyaz Kaya ,&nbsp;Mehmet Yildiz ,&nbsp;Sultan Sengul ,&nbsp;Ismail Ilker Kocaer ,&nbsp;Halil Erkan Sarıbas ,&nbsp;Orsan Gungor ,&nbsp;Ibrahim Tasal ,&nbsp;Yigit Can Aslan ,&nbsp;Serdar Dursun Ak ,&nbsp;Afsin Koker","doi":"10.1016/j.domaniend.2024.106855","DOIUrl":"10.1016/j.domaniend.2024.106855","url":null,"abstract":"<div><p>Freemartinism is the most common congenital anomaly among sexual disorders in dairy cows. This syndrome typically occurs in different-sex twin pregnancies and causes vascular anastomoses to form with the placenta in the early stages of fetal development. The study aims to determine the effectiveness of Anti-müllerian hormone (AMH) levels in calves and heifers of different age groups for diagnostic factors and to investigate the potential consequences of different hormone levels in different age groups on some liver biochemical parameters. The study involved 50 cattle from diverse age categories, divided into the freemartin group (FM Group, n=25) and the control group (C Group, n=25). Both FM and control groups were further divided into early-age (3-5 months), middle-aged (5-9 months), and older-aged groups (9-12 months). Serum AMH levels, along with total protein, albumin, and total cholesterol levels, were measured. While no statistically significant difference in AMH levels was observed in the early-age group (P:0.53), significant differences were determined in the middle (P:0.015) and older-age groups (P:0.01), where the FM group exhibited significantly decreased AMH levels compared to the control group. The evaluation of liver biochemistry revealed a statistically significant difference in total protein levels between the FM and control groups in the older age group (P:0.033). Consequently, it is reasonable to suggest that AMH levels may serve as a valid parameter for diagnosing freemartin syndrome in calves aged older than five months. Conversely, particularly in young calves, no significant differences in liver functionality were observed between freemartin-affected and healthy calves.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106855"},"PeriodicalIF":2.1,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141039973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The basic functions of rabbit ovarian granulosa cell are regulated by adipokines monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1","authors":"Alexander V. Sirotkin ,&nbsp;Zuzana Fabová ,&nbsp;Barbora Loncová ,&nbsp;Abdel Halim Harrath","doi":"10.1016/j.domaniend.2024.106856","DOIUrl":"10.1016/j.domaniend.2024.106856","url":null,"abstract":"<div><p>The aim of the present study was to examine the influence of monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1) on ovarian cell functions. Rabbit ovarian granulosa cells were cultured with or without MCP-1 or PAI-1 (at 0, 0.1, 1, or 10 ng/ml). Cell viability, proliferation, cytoplasmic apoptosis and release of progesterone and estradiol were measured by Cell Counting Kit-8 (CCK-8), BrdU incorporation, and cell death detection assays and ELISA. The addition of either MCP-1 or PAI-1 increased cell viability and proliferation and decreased apoptosis. MCP-1 promoted, while PAI-1 suppressed, progesterone release. Both MCP-1 and PAI-1 reduced estradiol output. The present results suggest that MCP-1 or PAI-1 can be physiological promoters of rabbit ovarian cell viability and proliferation, inhibitors of apoptosis and regulators of ovarian steroidogenesis.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106856"},"PeriodicalIF":2.1,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141036737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prostaglandin F2α treatment does not hasten ovulation in weaned sows","authors":"D.P. Fagundes ,&nbsp;M.S. Lucca ,&nbsp;B.G. Gasperin ,&nbsp;D. Missio ,&nbsp;M. Quirino ,&nbsp;A.P.G. Mellagi ,&nbsp;P.B.D. Gonçalves ,&nbsp;F.P. Bortolozzo ,&nbsp;R.R. Ulguim","doi":"10.1016/j.domaniend.2024.106854","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106854","url":null,"abstract":"<div><p>This study evaluated the efficiency of prostaglandin F2α (PGF) to hasten ovulation in weaned sows. In experiment I, weaned sows detected in estrus (0 h) received: no hormone (Control; <em>n</em> = 56); 0.5 mg PGF IM at 0 h and 2 h (PGF0; <em>n</em> = 56); or 0.5 mg PGF IM at 24 h and 26 h (PGF24; <em>n</em> = 55). In experiment II, weaned sows that did not express estrus signs until 72 h after weaning (0 h) were assigned to: no hormone (Control; <em>n</em> = 45); 10 µg buserelin acetate IM at 0 h (Buserelin; <em>n</em> = 43); 0.5 mg PGF IM at 34 h and 36 h (PGF; <em>n</em> = 44); or 10 µg buserelin acetate IM at 0 h plus 0.5 mg PGF IM at 34 h and 36 h (Buserelin + PGF; <em>n</em> = 45). In experiment I, no effect of PGF on the interval treatment onset to ovulation was observed (<em>P</em> &gt; 0.05), and no treatment effect was observed on the relative or cumulative proportion of females that ovulated post-treatment onset (<em>P</em> &gt; 0.05). In experiment II, treatment onset to ovulation interval was shorter for Buserelin group than for PGF group (<em>P</em> &lt; 0.05), and a higher cumulative percentage of Buserelin treated sows ovulated up to 48 h compared to PGF and Control groups (<em>P</em> &lt; 0.01), with no differences from Buserelin + PGF. Treatments did not affect total number of piglets born in both experiments (<em>P</em> &gt; 0.05). In conclusion, PGF did not hasten ovulation timing or affect litter size in weaned sows.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106854"},"PeriodicalIF":2.1,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early induction of luteolysis in a timed AI protocol increases reproductive performance in beef cows","authors":"Eduardo P. Silva ,&nbsp;Alfredo Q. Antoniazzi ,&nbsp;João B.S. Borges ,&nbsp;Pedro L.J. Monteiro ,&nbsp;Amanda B. Machado ,&nbsp;Marcelo M. Dias ,&nbsp;Valério V.M. Portela Junior ,&nbsp;Milo C. Wiltbank","doi":"10.1016/j.domaniend.2024.106853","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106853","url":null,"abstract":"<div><p>The aim of this study was to produce a longer proestrus by early administration of prostaglandin F2α (PGF) in a timed artificial insemination (TAI) protocol in non-suckling <em>Bos taurus</em> (Angus crossbreed) beef cows. On day 0, cows (n = 489) were treated with an intravaginal 1 g progesterone (P4) device and 2 mg of estradiol benzoate. On day 7, cows were randomized into two groups: PGF7(n = 244; 500 µg of sodium cloprostenol 24 h before P4 device removal) or PFG8 (n = 245; 500 µg of sodium cloprostenol at P4 device removal). On day 8, P4 device was removed and cows received 0.5 mg of estradiol cypionate. All cows were submitted to TAI on day 10 (48–50 hours after P4 device removal). Cows treated with PGF on day 7 had greater expression of estrus (91.3 vs 79.1 %; P = 0.0011), regardless of CL presence at beginning of the protocol. Cows from PGF7 group had lower circulating P4 concentrations on day 8 in comparison with PGF8 treated cows (1.86 vs 2.99 ng/mL; P &lt; 0.001). However, preovulatory follicle diameter did not differ among treatments at TAI (11.9 vs 11.8 mm; P = 0.7881). Pregnancy per TAI (P/TAI) was greater for PGF7 (63.9 vs 50.6 %; P = 0.0114) than PGF8 treated cows. In cows with follicles &lt;8.5 mm at TAI, expression of estrus (33.3 vs 26.6 %; P = 0.6427) and P/TAI (40 vs 26.6 %; P = 0.3657) were low in both PGF7 and PGF8 treated cows, respectively. In cows with medium follicle size (8.5 to 11.9 mm) PGF7 treated cows had greater expression of estrus (90.5 vs 80 %; P = 0.033) and P/TAI (62.2 vs 49 %; P = 0.053). In cows with follicles &gt;12 mm, expression of estrus was greater for PGF7 than PGF8 treated cows (99.1 vs 93.3 %; P = 0.045), however P/TAI did not differ (68.2 vs 59 %; P = 0.149). In cows with P4 &lt; 1.99 ng/mL on day 8, expression of estrus was similar between PGF7 and PGF8 treated cows (92.6 vs 90.4 %; P = 0.53), and P/TAI tended to be greater for PGF7 than PGF8 treated cows (63 vs 52.1 % P = 0.076). However, in cows with P4 &gt; 2 ng/mL PGF7 cows had higher expression of estrus (89 vs 67.5 %; P = 0.0005) and P/TAI (64.8 vs 48.7 %; P = 0.021) than PGF8. Thus, increasing the proestrous period by inducing luteolysis 24 hours earlier than removing the P4 intravaginal device enhanced fertility in non-suckling cyclic beef cows by increasing expression of estrus and P/TAI.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106853"},"PeriodicalIF":2.1,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of somatotrophic hormones in the postpartum regulation of ovarian activity in mares","authors":"Maria Melchert ,&nbsp;Jörg Aurich ,&nbsp;Reinhard Ertl ,&nbsp;Ursula Reichart ,&nbsp;Ingrid Walter ,&nbsp;Camille Gautier ,&nbsp;Martim Kaps ,&nbsp;Christine Aurich","doi":"10.1016/j.domaniend.2024.106852","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106852","url":null,"abstract":"<div><p>Mares resume ovarian activity rapidly after foaling. Besides follicle-stimulating hormone (FSH) and luteinizing hormone (LH), the pituitary synthesizes prolactin and growth hormone which stimulate insulin-like growth factor (IGF) synthesis in the liver. We tested the hypothesis that follicular growth is initiated already antepartum, mares with early and delayed ovulation differ in IGF-1 release and that there is an additional IGF-1 synthesis in the placenta. Plasma concentrations of LH, FSH, IGF-1, IGF-2, activin and prolactin. IGF-1, IGF-2, prolactin and their receptors in placental tissues were analyzed at the mRNA and protein level. Follicular growth was determined from 15 days before to 15 days after foaling in 14 pregnancies. Mares ovulating within 15 days postpartum formed group OV (n=5) and mares not ovulating within 15 days group NOV (n=9). Before foaling, follicles with a diameter &gt;1 cm were present in all mares and their number increased over time (p&lt;0.05). Follicle growth after foaling was more pronounced in OV mares (day p&lt;0.001, group p&lt;0.05, day x group p&lt;0.05) in parallel to an increase in LH concentration (p&lt;0.001, day x group p&lt;0.001) while FSH increased (p&lt;0.001) similarly in both groups. Plasma concentrations of IGF-1 and prolactin peaked one day after foaling (p&lt;0.001). The IGF-1 mRNA abundance was higher in the allantochorion but lower in the amnion of OV versus NOV mares (group p=0.01, localization x group p&lt;0.01). The IGF-1 receptor mRNA was most abundant in the allantochorion (p&lt;0.001) and IGF-1 protein was expressed in placental tissue without differences between groups. In conclusion, follicular growth in mares is initiated before foaling and placental IGF-1 may enhance resumption of ovulatory cycles.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106852"},"PeriodicalIF":2.1,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140825486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of hormones in the regulation of lactogenic immunity in porcine and bovine species","authors":"Kexin Hua ,&nbsp;Dan Liu ,&nbsp;Qianshuai Xu ,&nbsp;Yuna Peng ,&nbsp;Yu Sun ,&nbsp;Rongrong He ,&nbsp;Rui Luo ,&nbsp;Hui Jin","doi":"10.1016/j.domaniend.2024.106851","DOIUrl":"10.1016/j.domaniend.2024.106851","url":null,"abstract":"<div><p>Colostrum and milk offer a complete diet and vital immune protection for newborn mammals with developing immune systems. High immunoglobulin levels in colostrum serve as the primary antibody source for newborn piglets and calves. Subsequent milk feeding support continued local antibody protection against enteric pathogens, as well as maturation of the developing immune system and provide nutrients for newborn growth. Mammals have evolved hormonal strategies that modulate the levels of immunoglobulins in colostrum and milk to facilitate effective lactational immunity. In addition, hormones regulate the gut-mammary gland-secretory immunoglobulin A (sIgA) axis in pregnant mammals, controlling the levels of sIgA in milk, which serves as the primary source of IgA for piglets and helps them resist pathogens such as PEDV and TGEV. In the present study, we review the existing studies on the interactions between hormones and the gut-mammary-sIgA axis/lactogenic immunity in mammals and explore the potential mechanisms of hormonal regulation that have not been studied in detail, to draw attention to the role of hormones in influencing the immune response of pregnant and lactating mammals and their offspring, and highlight the effect of hormones in regulating sIgA-mediated anti-infection processes in colostrum and milk. Discussion of the relationship between hormones and lactogenic immunity may lead to a better way of improving lactogenic immunity by determining a better injection time and developing new vaccines.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106851"},"PeriodicalIF":2.1,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140760014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial local expressions of kisspeptin in the uterus and uterine tubes and its relationship to the reproductive potential in goats","authors":"Haney Samir ,&nbsp;Ahmed Elfadadny ,&nbsp;Faten Radwan ,&nbsp;Hossam R. El-Sherbiny ,&nbsp;Ayman A. Swelum ,&nbsp;Wael A. Khalil ,&nbsp;Gen Watanabe","doi":"10.1016/j.domaniend.2024.106850","DOIUrl":"10.1016/j.domaniend.2024.106850","url":null,"abstract":"<div><p>Kisspeptins are neuropeptides encoded by the <em>Kiss1</em> gene that was discovered as a metastasis suppressor gene in melanoma and breast cancer. Kisspeptin has pivotal functions for gonadotropin-releasing hormone secretion and plays integrated roles in the hypothalamic-pituitary-gonadal axis. However, little is known about the peripheral expression of kisspeptin in ruminants, especially in the female reproductive tract. Here, the objectives of the current study were to investigate the spatial localization of kisspeptin and mRNA expression of Kiss1 and its receptor (Kiss1r) in the fallopian tubes (FT) and uterus of goats at varied reproductive activity (cyclic versus true anoestrous goats, n=6, each). Specimens of the uterus and FT were collected and fixed using paraformaldehyde to investigate the localizations of kisspeptin in the selected tissues by immunohistochemistry. Another set of samples was snape-frozen to identify the expressions of mRNAs encoding Kiss1 and Kiss1r using real-time PCR. Results revealed immunolocalizations of kisspeptin in the uterus and the FT. The staining of kisspeptin was found mainly in the mucosal epithelium of the uterus the FT, and the endometrial glands. Very intense staining of kisspeptin was found in the uterine and FT specimens in the true anoestrous goats compared to that in cyclic ones. The expression of mRNA encoding <em>Kiss1</em> gene was significantly higher in the uterine specimen of cyclic goats (1.00±0.09) compared to that in the true anoestrous goats (0.62±0.08) (P ˂0.05), while the expression of mRNA encoding <em>Kiss1r</em> was significantly (P ˂0.001) higher in the uterine tissues of true anoestrous goats (1.78±0.17) compared to that in cyclic ones (1.00±0.11). In conclusion, immunohistochemical localization of kisspeptin and the expression of mRNA encoding <em>Kiss1/Kiss1r</em> revealed spatial changes in the uterus and FT of goats according to the reproductive potential of goats (cyclic versus true anoestrous goats). However, the definitive local role of kisspeptin in the uterus and FT need further investigation.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106850"},"PeriodicalIF":2.1,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140608866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Birth weight, growth indices, and seminal parameters in male offspring are resilient features to maternal pre-conceptional dietary manipulation in sheep","authors":"Cesar A. Rosales-Nieto ,&nbsp;Bradford W. Daigneault ,&nbsp;Jennifer N. Roberts ,&nbsp;Rodrigo Sánchez-López ,&nbsp;Barbara Makela ,&nbsp;Yong Pu ,&nbsp;Richard Ehrhardt ,&nbsp;João H. Jabur Bittar ,&nbsp;Almudena Veiga-Lopez","doi":"10.1016/j.domaniend.2024.106849","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106849","url":null,"abstract":"<div><p>Gestational diet manipulation can lead to inadequate fetal nutrient supply resulting in low birth weight, limited postnatal growth, and consequently, reduced reproductive performance in the progeny. However, effects of short-term maternal pre-conceptional dietary manipulation on postnatal growth and reproductive parameters of male offspring in large animals remains unexplored. To determine these consequences, female crossbred (Polypay x Dorset) sheep were allocated to three groups (<em>n</em> = 33/group) of dietary manipulation for 21 days prior to mating under the following conditions: (1) control at 100 % of maintenance energy requirements (40 Kcal of metabolizable energy/kg body weight [BW]), (2) undernutrition (UN) at 50 % of Control intake, and (3) overnutrition (ON) at 200 % of maintenance energy. Singleton ram lambs (UN:9; C:12; ON:6) were monitored from birth until 8 months of age, including birth weight, weekly weights, weight gain, body mass index (BMI), and circulating testosterone. After weaning, monthly scrotal circumference and subcutaneous fat depth were measured. Semen morphology and motility were evaluated at 7 and 8 months of age. Birth weight, weight gain, and BMI at birth and weaning were not significantly different among nutritional treatments. None of the pre-conceptional diets affected body weight change from weaning until 36 weeks of age, BMI, fat depth, or scrotal circumference across the experiment. A sustained rise in plasma testosterone concentrations was detected when ram lambs were, on average, 82 days old and 37 kg. Both testosterone concentrations and scrotal circumference were positively correlated to body weight regardless of treatment group. In addition, seminal parameters did not differ among treatments, but a transient increase in plasma testosterone at 18 weeks of age was observed in ON ram lambs compared to control rams. In conclusion, birth weight, growth indices, and seminal parameters in singleton rams are resilient features in the progeny upon maternal pre-conceptional dietary manipulation in sheep.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106849"},"PeriodicalIF":2.1,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0739724024000122/pdfft?md5=13b68f755ef6eb47d3602f4feb1a29af&pid=1-s2.0-S0739724024000122-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140545764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin interacts with PPARγ agonists to promote bovine adipocyte differentiation","authors":"Pan−Pan Guo ,&nbsp;Xue−Rui Yao ,&nbsp;Yong−Nan Xu ,&nbsp;Xin Jin ,&nbsp;Qiang Li ,&nbsp;Chang−Guo Yan ,&nbsp;Nam−Hyung Kim ,&nbsp;Xiang−Zi Li","doi":"10.1016/j.domaniend.2024.106848","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106848","url":null,"abstract":"<div><p>Insulin is a potent adipogenic hormone that triggers a series of transcription factors that regulate the differentiation of preadipocytes into mature adipocytes. Ciglitazone specifically binds to peroxisome proliferator−activated receptor−<em>γ</em> (<em>PPARγ</em>), thereby promoting adipocyte differentiation. As a natural ligand of <em>PPARγ</em>, oleic acid (OA) can promote the translocation of <em>PPARγ</em> into the nucleus, regulate the expression of downstream genes, and promote adipocyte differentiation. We hypothesized that ciglitazone and oleic acid interact with insulin to enhance bovine preadipocyte differentiation. Preadipocytes were cultured 96 h in differentiation medium containing 10 mg/L insulin (I), 10 mg/L insulin + 10 µM cycloglitazone (IC), 10 mg/L insulin + 100 µM oleic acid (IO), or 10 mg/L insulin + 10 µM cycloglitazone+100 µM oleic acid (ICO). Control preadipocytes (CON) were cultured in differentiation medium (containing 5% fetal calf serum). The effects on the differentiation of Yanbian cattle preadipocytes were examined using molecular and transcriptomic techniques, including differentially expressed genes (DEGs) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis. I, IC, IO, and ICO treatments produced higher concentrations of triglycerides (TAG) and lipid droplet accumulation in preadipocytes compared with CON treatment (<em>P</em> &lt; 0.05). Co−treatment of insulin and <em>PPARγ</em> agonists significantly increased the expression of genes involved in regulating adipogenesis and fatty acid synthesis. (<em>P</em> &lt; 0.05). Differential expression analysis identified 1488, 1764, 1974 and 1368 DEGs in the I, IC, IO and ICO groups, respectively. KEGG pathway analysis revealed DEGs mainly enriched in <em>PPAR</em> signalling, <em>FOXO</em> signaling pathway and fatty acid metabolism. These results indicate that OA, as <em>PPARγ</em> agonist, can more effectively promote the expression of bovine lipogenesis genes and the content of TAG and adiponectin when working together with insulin, and stimulate the differentiation of bovine preadipocytes. These findings provide a basis for further screening of relevant genes and transcription factors in intramuscular fat deposition and meat quality to enhance breeding programs.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106848"},"PeriodicalIF":2.1,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140341348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold exposure affects glucose metabolism, lipid droplet deposition and mitophagy in skeletal muscle of newborn goats","authors":"Duo Su ,&nbsp;Yulong Song ,&nbsp;Die Li ,&nbsp;Shizhong Yang ,&nbsp;Siyuan Zhan ,&nbsp;Tao Zhong ,&nbsp;Jiazhong Guo ,&nbsp;Jiaxue Cao ,&nbsp;Li Li ,&nbsp;Hongping Zhang ,&nbsp;Linjie Wang","doi":"10.1016/j.domaniend.2024.106847","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106847","url":null,"abstract":"<div><p>Cold exposure is a common stressor for newborn goats. Skeletal muscle plays an important role in maintaining whole-body homeostasis of glucose and lipid metabolism. However, the molecular mechanisms underlying regulation of skeletal muscle of newborn goats by cold exposure remains unclear. In this study, we found a significant increase (<em>P</em> &lt; 0.01) in serum glucagon levels after 24 h of cold exposure (COLD, 6°C), while glucose and insulin concentrations were significantly decreased (<em>P</em> &lt; 0.01) compared to room temperature (RT, 25°C). Additionally, we found that cold exposure reduced glycogen content (<em>P</em> &lt; 0.01) in skeletal muscle. Pathway enrichment analysis revealed that cold exposure activated skeletal muscle glucose metabolism pathways (including insulin resistance and the insulin signaling pathway) and mitophagy-related pathways. Cold exposure up-regulated the expression of genes involved in fatty acid and triglyceride synthesis, promoting skeletal muscle lipid deposition. Notably, cold exposure induced mitophagy in skeletal muscle.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106847"},"PeriodicalIF":2.1,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}