The basic functions of rabbit ovarian granulosa cell are regulated by adipokines monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1

IF 1.9 2区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Alexander V. Sirotkin , Zuzana Fabová , Barbora Loncová , Abdel Halim Harrath
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引用次数: 0

Abstract

The aim of the present study was to examine the influence of monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1) on ovarian cell functions. Rabbit ovarian granulosa cells were cultured with or without MCP-1 or PAI-1 (at 0, 0.1, 1, or 10 ng/ml). Cell viability, proliferation, cytoplasmic apoptosis and release of progesterone and estradiol were measured by Cell Counting Kit-8 (CCK-8), BrdU incorporation, and cell death detection assays and ELISA. The addition of either MCP-1 or PAI-1 increased cell viability and proliferation and decreased apoptosis. MCP-1 promoted, while PAI-1 suppressed, progesterone release. Both MCP-1 and PAI-1 reduced estradiol output. The present results suggest that MCP-1 or PAI-1 can be physiological promoters of rabbit ovarian cell viability and proliferation, inhibitors of apoptosis and regulators of ovarian steroidogenesis.

单核细胞趋化蛋白-1和纤溶酶原激活物抑制剂-1调节兔卵巢颗粒细胞的基本功能
本研究旨在探讨单核细胞趋化蛋白-1(MCP-1)和纤溶酶原激活物抑制剂-1(PAI-1)对卵巢细胞功能的影响。用或不用 MCP-1 或 PAI-1(0、0.1、1 或 10 纳克/毫升)培养兔卵巢颗粒细胞。细胞活力、增殖、细胞质凋亡以及孕酮和雌二醇的释放均通过细胞计数试剂盒-8(CCK-8)、BrdU掺入、细胞死亡检测法和酶联免疫吸附法进行测定。添加 MCP-1 或 PAI-1 都会增加细胞活力和增殖,减少细胞凋亡。MCP-1 促进了孕酮的释放,而 PAI-1 则抑制了孕酮的释放。MCP-1 和 PAI-1 都会减少雌二醇的输出。本研究结果表明,MCP-1 或 PAI-1 可作为兔卵巢细胞活力和增殖的生理促进剂、细胞凋亡的抑制剂和卵巢类固醇生成的调节剂。
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来源期刊
Domestic animal endocrinology
Domestic animal endocrinology 农林科学-奶制品与动物科学
CiteScore
5.50
自引率
4.80%
发文量
58
审稿时长
31 days
期刊介绍: Domestic Animal Endocrinology publishes scientific papers dealing with the study of the endocrine physiology of domestic animal species. Those manuscripts utilizing other species as models for clinical or production problems associated with domestic animals are also welcome. Topics covered include: Classical and reproductive endocrinology- Clinical and applied endocrinology- Regulation of hormone secretion- Hormone action- Molecular biology- Cytokines- Growth factors
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