Domestic animal endocrinology最新文献

{"title":"Anti-Müllerian hormone in female dromedary camel and its association with super-ovulatory response in embryo donors","authors":"F. Seyedasgari ,&nbsp;L. Melado Vidales ,&nbsp;A. Souza ,&nbsp;B. Lawrenz ,&nbsp;J. Sibal ,&nbsp;H. Fatemi ,&nbsp;B. Asadi","doi":"10.1016/j.domaniend.2023.106818","DOIUrl":"10.1016/j.domaniend.2023.106818","url":null,"abstract":"<div><p>Anti-Müllerian hormone (AMH) has a conserved role in regulating the reproductive cycle in several species. Its circulating concentration reflects the size of the growing primordial follicle reserve and is a reliable predictor of superovulation response in embryo/oocyte donors. This study investigated the possible application of AMH measurement in dromedary camels (Camelus dromedarius) multiple ovulation embryo transfer programs. In experiment 1, the follicular cycle of synchronized and naturally cycling camels (n = 12) was monitored. Blood was collected at 6 timepoints in 2 consecutive cycles corresponding to emergence, mid-cycle, and dominance in both group and hormonal fluctuations were evaluated for repeatability of measurements within and between cycles. In experiment 2, the correlation between circulating AMH concentrations prior to initiation of superovulation and the outcome of superovulation was evaluated. The results were compared between donors with higher (n = 7) and lower than median (n = 8) AMH values. Mean AMH concentrations in synchronized and non-synchronized camels were 1.46 ± 0.15 and 0.95 ± 0.09, respectively. Intercycle and intracycle values of AMH showed high repeatability in camels of both groups (&gt;96.4% and &gt;92.74%, respectively) with significant correlations between values at different stages of the ovarian cycle (Emergence and mid-cycle: R² = 0.82; emergence and. dominance: R² = 0.86; Mid-cycle and dominance: R² = 0.93, <em>P</em> &lt; 0.05). Total follicles, CLs, and recovered embryos were highly correlated with AMH values prior to superovulation (R² = 0.64, R² = 0.77, and R² = 0.64, respectively, <em>P</em> &lt; 0.05). A greater number of developed follicles prior to mating (17.00 ± 2.09 vs. 7.62 ± 1.06), CLs (12.58 ± 1.36 vs. 5.12 ± 0.93), transferable (10.85 ± 1,31 vs. 3.37 ± 0.82), and spherical embryos (8.14 ± 1.07 vs. 2.62 ± 0.7) were observed in camels with higher than median concentrations of AMH (<em>P</em> &lt; 0.05). Fluctuations in estradiol and progesterone did not affect variations in mean AMH values (r² &lt; 0.19 and r² &lt; 0.24, respectively, <em>P</em> &gt; 0.05). In conclusion, highly consistent AMH values in dromedary camels are a reliable predictor of superovulation response and outcome in dromedary camels.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"86 ","pages":"Article 106818"},"PeriodicalIF":2.1,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41114021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of progesterone and estrone-sulfate in feces of American Bison using liquid chromatography coupled to mass spectrometry: Technical validation and correlation with blood levels","authors":"Patrice Dufour ,&nbsp;Vincent Frisée ,&nbsp;Goulven Rigaux ,&nbsp;Flore Brutinel ,&nbsp;Sophie Egyptien ,&nbsp;Philippe Bossaert ,&nbsp;Jessica Deleersnyder ,&nbsp;Stéfan Deleuze ,&nbsp;Stéphanie Peeters ,&nbsp;Caroline Le Goff ,&nbsp;Jérôme Ponthier ,&nbsp;Etienne Cavalier","doi":"10.1016/j.domaniend.2023.106819","DOIUrl":"10.1016/j.domaniend.2023.106819","url":null,"abstract":"<div><p>American Bison's wild nature limits blood sample availability to study its endocrinology. This report describes progesterone (P4) and estrone-sulfate (E1S) assays in American Bison feces using Liquid Chromatography coupled with Mass Spectrometry (LC-MS). In 2 ranches, samples of feces (n = 73) and serum (n = 93) were collected in pregnant and nonpregnant American Bison. Feces samples (250 mg) were extracted with methanol, purified, and concentrated. Then, feces and serum samples were assayed using LC-MS, according to our previously described technique. Fecal matrix homogeneity was determined by measuring steroids in different areas of the sample and concentration evolutions were evaluated after storage at room temperature. During the validation process, lower limits of quantification were 20 pg/g (E1S) and 4 ng/g (P4) by meeting the following criteria: relative standard deviation &lt;15% and relative bias &lt;15%. By measuring hormones in different spots from the same sample, a moderate variability for E1S (coefficient of variation [CV] up to 21.3%) and a high variability for P4 (CV up to 85.5%) were highlighted. Correlation between concentrations in feces and in serum was higher for E1S (r = 0.77) than for P4 (r = 0.65) and P4 could be assayed in pregnant and nonpregnant animals whereas E1S was only present in pregnant. Feces storage at room temperature induced modification of steroid concentrations. The quantification of E1S and, at a lower level, of P4 in feces is an interesting alternative to serum assay to describe the pregnancy-related evolution of these steroids in American Bisons, with feces ideally stored frozen and mixed before the LC-MS procedures.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"86 ","pages":"Article 106819"},"PeriodicalIF":2.1,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41114020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Is an increase of glucocorticoid concentrations related to the degree of arousal or valence experienced by an animal to a stimulus?” [Domestic Animal Endocrinology 81(2022) 1-7/106752]","authors":"O.S. Iyasere ,&nbsp;S.O. Durosaro ,&nbsp;V.J. Oyeniran ,&nbsp;J.O. Daramola","doi":"10.1016/j.domaniend.2022.106774","DOIUrl":"10.1016/j.domaniend.2022.106774","url":null,"abstract":"","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"84 ","pages":"Article 106774"},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10026826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship among cattle breed and anabolic implant protocol relative to feedlot performance: Growth, temperament, feeding behavior, carcass traits, and economic return","authors":"C.C. Reichhardt ,&nbsp;S.A. Bayles ,&nbsp;R. Feuz ,&nbsp;L.A. Motsinger ,&nbsp;A.F. Alberto ,&nbsp;L.L. Okamoto ,&nbsp;B.L. Brown ,&nbsp;R.K. Briggs ,&nbsp;B.W. Roholt ,&nbsp;B.R. Bowman ,&nbsp;R. Larsen ,&nbsp;M.D. Garcia ,&nbsp;K.J. Thornton","doi":"10.1016/j.domaniend.2023.106806","DOIUrl":"10.1016/j.domaniend.2023.106806","url":null,"abstract":"<div><p>Recent research has suggested that different cattle breed types may respond differently to anabolic implant protocols of varying intensity. Therefore, the purpose of this research was to compare anabolic implant protocols in feedlot steers of 2 different breed types. Sixty steers were stratified by weight and breed in a 2 × 3 factorial design examining 2 different breeds: Angus (AN; n=38) or Santa Gertrudis influenced (SG; n=22), and 3 implant strategies: no implant (CON; n=20), a moderate intensity implant protocol (d0 implant: Revalor-G, d56 implant: Revalor-IS, d112 implant: Revalor-S; MI; n=20), or a high intensity implant protocol (d0 implant: Revalor-IS, d56 implant: Revalor-S, d112 implant: Revalor-200; HI; n=20). Steers were randomly placed into pens equipped with GrowSafe bunks to collect dry matter intake and feeding behavior. All animals were fed the same diet. Weight, chute score, exit velocity, serum, rectal temperature, hip height and 12th rib fat thickness were collected approximately every 28 d over a 196 d period. Serum urea nitrogen (SUN) was evaluated as well. Total average daily gain was increased (<em>P &lt; 0.0001</em>) in both the HI and MI steers compared to the CON steers by 29.4% and 26%, respectively. A treatment × breed interaction was observed (<em>P &lt; 0.0001</em>) for hip height, with AN-CON steers being shorter (<em>P &lt; 0.0007</em>) than AN-HI, SG-CON, SG-MI, and SG-HI steers. A breed × treatment interaction was observed (<em>P &lt; 0.004</em>) for chute score and rectal temperature, with SG-HI and SG-MI steers having increased chute scores (<em>P &lt; 0.001</em>) when compared to AN-HI, AN-MI, AN-CON, and SG-CON throughout the course of the trial. Additionally, SG-HI and SG-MI steers had an increased rectal temperature (<em>P &lt; 0.004</em>) compared to AN-HI, AN-MI, AN-CON, and SG-CON steers. A breed effect was observed (<em>P = 0.002</em>) for SUN with AN steers having increased (<em>P = 0.002</em>) SUN concentration compared to SG sired steers, in addition to a treatment effect (<em>P &lt; 0.0001</em>), with CON steers having a higher (<em>P &lt; 0.0001</em>) SUN concentration than MI and HI steers, regardless of breed. The MI implant protocol increased net return per head, on average, by $97.28, regardless of breed, while the HI implant protocol increased net return by only $80.84. Taken together, despite the cattle breed types responding differently to the different anabolic implant protocols at times, a moderate intensity anabolic implant protocol was optimal in this experiment for steers raised in a temperate climate.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"84 ","pages":"Article 106806"},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10083710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA miR-152 can support ovarian granulosa cell functions and modify apigenin actions","authors":"Z. Fabová ,&nbsp;Z. Kislíková ,&nbsp;B. Loncová ,&nbsp;M. Bauer ,&nbsp;A.H. Harrath ,&nbsp;A.V. Sirotkin","doi":"10.1016/j.domaniend.2023.106805","DOIUrl":"10.1016/j.domaniend.2023.106805","url":null,"abstract":"<div><p>The study aimed to evaluate the involvement of apigenin, microRNA (miR)-152, and their interrelationships in the control of basic ovarian granulosa cell functions. The effects of apigenin (0, 10, and 100 µg/mL), miR-152 analogues or miR-152 inhibitor, and their combinations with apigenin on porcine granulosa cells were examined. Expression levels of miR-152, viability, proliferation, apoptosis, steroid hormones, IGF-I, oxytocin, and prostaglandin E2 release were analyzed. Apigenin increased the expression of miR-152, cell proliferation, and estradiol release and reduced apoptosis, progesterone, and IGF-I output. MicroRNA-152 analogues promoted cell viability and proliferation, as well as the release of progesterone, IGF-I, oxytocin, and prostaglandin E2; however, it inhibited apoptosis and estradiol output. miR-152 inhibitor had the opposite effect. Moreover, miR-152 analogues suppressed the effect of apigenin on cell apoptosis and estradiol release. These observations 1) confirm the involvement of apigenin in the control of basic ovarian cell functions; 2) are the first demonstration of importance of miR-152 in the control of these functions; 3) show the ability of apigenin to promote miR-152 expression and the ability of miR-152 to modify apigenin effects on ovarian cells.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"84 ","pages":"Article 106805"},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10017583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hair cortisol concentration in postpartum dairy cows and its association with parameters of milk production","authors":"Winfried Otten ,&nbsp;Susen Heimbürge ,&nbsp;Armin Tuchscherer ,&nbsp;Ellen Kanitz","doi":"10.1016/j.domaniend.2023.106792","DOIUrl":"10.1016/j.domaniend.2023.106792","url":null,"abstract":"<div><p>Hair cortisol concentration (HCC) is considered as an indicator for a minimally invasive assessment of long-term stress. In dairy cows, in addition to stress influences, changing physiological conditions during gestation and lactation (eg, due to varying energy requirements or fluctuating milk yield) may affect HCCs. Thus, the aim of our study was to investigate HCCs of dairy cows during different stages of lactation and to determine the relationship between milk production traits and hair cortisol levels. Samples of natural hair and regrown hair were collected from 41 multiparous Holstein Friesian cows at 100-d intervals from parturition to 300 d postpartum. All samples were analyzed for cortisol concentration and the association of HCC with milk productions traits was evaluated. Our results show that cortisol concentration in natural hair increased after parturition and was highest 200 d postpartum. Cumulative milk yield from parturition to 300 d showed moderate and positive correlation with HCC in natural hair at 300 d. There was a positive correlation between urea concentration in milk and cortisol levels in regrown hair at 200 d, and between somatic cell count in milk and HCC in natural and regrown hairs 200 d postpartum. Together, these findings suggest that physiological loads during lactation, eg, caused by metabolic stress and/or inflammation, may be associated with increased HCC levels. In addition, the results on hair color confirm previous findings in cattle that black hair has higher cortisol concentrations than white hair. Black hair therefore appears to be more suitable for hair cortisol analysis as it provides higher protection against photodegradation.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"84 ","pages":"Article 106792"},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10024072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-acting injectable progesterone treatment prior to puberty induction in gilts","authors":"P. Baldessar ,&nbsp;C.R.C. de Brito ,&nbsp;H. Johann ,&nbsp;C. Schultz ,&nbsp;V. Peripolli ,&nbsp;F. Moreira ,&nbsp;T. Lucia Jr. ,&nbsp;R.R. Ulguim ,&nbsp;B.G. Gasperin ,&nbsp;I. Bianchi","doi":"10.1016/j.domaniend.2023.106807","DOIUrl":"10.1016/j.domaniend.2023.106807","url":null,"abstract":"<div><p>Progesterone (P4) has a pivotal role on female puberty attainment in most farm animals. However, there are no studies evaluating the effect of P4 treatment previously to boar exposure for puberty induction in gilts. Therefore, serum P4 concentration, estrus expression and reproductive performance after boar stimuli were evaluated in gilts intramuscularly treated with long-acting P4 before boar exposure. In Experiment I, prepubertal gilts received either 1 mL of saline (control) or intramuscular (I.M.) P4 treatment (150 mg, 300 mg or 600 mg; <em>n</em> = 6 per treatment). Serum P4 concentration for P4-treated gilts was greater than for control gilts for at least 8 d for P4300 and P4600 groups (<em>P</em> &lt; 0.05), but greater until after 16 d only for those treated with 600 mg (<em>P</em> &lt; 0.05). In Experiments II (prepubertal) and III (peripubertal), gilts received either saline (control) or 300 mg P4 I.M. and those showing estrus signs were artificially inseminated (AI), whereas gilts without estrus expression were culled. In prepubertal gilts (Exp. II), estrus expression rate did not differ (<em>P</em> &lt; 0.05) for control (79.1%; <em>n</em> = 110) and P4-treated gilts (81.5%; <em>n</em> = 108). In peripubertal gilts (Exp. III), although estrus expression did not differ between control (77.6%; <em>n</em> = 106) and P4-treated (69.6%; <em>n</em> = 102) gilts (<em>P</em> &gt; 0.05), P4-treated gilts presented longer (23.1 ± 1.4 days) interval from treatment to estrus expression than control gilts (17.1 ± 1.3 days; <em>P</em> &lt; 0.05). In Experiments II and III, the proportion of culled gilts with ovarian structures consistent with normal estrous cycles, farrowing rate, and litter size did not differ between treatments (<em>P</em> &gt; 0.05). In conclusion, I.M. treatment with 300 or 600 mg of long-acting P4 was efficient in maintaining high P4 concentrations in prepubertal gilts for at least 8 days. However, P4 treatment over this time interval did not benefit the reproductive performance of prepubertal and peripubertal gilts.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"84 ","pages":"Article 106807"},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10026548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental and hormonal regulation of FBN1 and OR4M1 mRNA in bovine granulosa cells","authors":"E.R.S. Maylem ,&nbsp;L.J. Spicer ,&nbsp;I.M. Batalha ,&nbsp;L.F. Schütz","doi":"10.1016/j.domaniend.2023.106791","DOIUrl":"10.1016/j.domaniend.2023.106791","url":null,"abstract":"<div><p>Recent studies have reported hormonal regulation of expression of <em>fibrillin 1</em> (<em>FBN1)</em>, the gene that encodes asprosin, in bovine theca cells, however, hormonal regulation of gene expression of <em>FBN1</em> and the asprosin receptor, <em>olfactory receptor 4M1</em> (<em>OR4M1),</em> has not been evaluated in granulosa cells (GC). This study was designed to characterize <em>FBN1</em> and <em>OR4M1</em> gene expression in GC during development of bovine dominant ovarian follicles, and to determine the hormonal regulation of <em>FBN1</em> and <em>OR4M1</em> mRNA expression in GC. GC <em>FBN1</em> mRNA abundance was greater <em>(P</em> &lt; 0.05) in medium (5.1–8 mm) estrogen inactive (EI) follicles than in large (&gt;8.1 mm) or small (1–5 mm) EI follicles. In comparison, GC <em>OR4M1</em> mRNA abundance was greater (<em>P</em> &lt; 0.05) in small EI follicles than in large or medium EI follicles. Abundance of <em>OR4M1</em> mRNA in GC of follicles collected on days 3 to 4 (early growth phase) and on days 5 to 6 (late growth phase) was similar, whereas <em>FBN1</em> mRNA abundance was greater (<em>P</em> &lt; 0.05) on days 5 to 6 vs days 3 to 4. Hormonal regulators for <em>FBN1</em> mRNA abundance in cultured small-follicle GC were identified: TGFβ1 causing a 2.45-fold increase, WNT3A causing a 1.45-fold increase, and IGF1 causing a 65% decrease. Steroids, leptin, insulin, growth hormone, follicle stimulating hormone, fibroblast growth factor 9 and epidermal growth factor had no effect on <em>FBN1</em> mRNA abundance. Abundance of <em>OR4M1</em> mRNA in GC was regulated by progesterone with 3.55-fold increase, but other hormones did not affect GC <em>OR4M1</em> mRNA abundance. Findings indicate that both <em>FBN1</em> and <em>OR4M1</em> gene expression are hormonally and developmentally regulated in bovine follicles, and thus may affect asprosin production and its subsequent role in ovarian follicular function in cattle.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"84 ","pages":"Article 106791"},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10029071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of FSH stimulation and age on the ovarian and uterine traits and histomorphometry of prepubertal gilts","authors":"V.A.P. Alfradique ,&nbsp;D.L. Souza Netto ,&nbsp;S.V.P. Alves ,&nbsp;A.F. Machado ,&nbsp;C.M. Novaes ,&nbsp;J.M. Penitente-Filho ,&nbsp;M. Machado-Neves ,&nbsp;M.S. Lopes ,&nbsp;S.E.F. Guimarães","doi":"10.1016/j.domaniend.2023.106786","DOIUrl":"10.1016/j.domaniend.2023.106786","url":null,"abstract":"<div><p>This study investigated the effect of age and follicle stimulating hormone (FSH) treatment on the estradiol (E2) plasma concentration, ovarian follicle development, endometrial histomorphometry, and ultrasonographic parameters of the ovaries and uterus in prepubertal gilts. Thirty-five prepubertal gilts were grouped according to age (140 or 160 d), and within each age, gilts were allotted to receive 100 mg of FSH (treated; G140 + FSH [n = 10] and G160 + FSH [n = 7]) or saline solution (control; G140 + control [n = 10] and G160 + control [n = 8]). The total dose of FSH was divided into 6 similar doses administered every 8 h (days 0–2). Before and after FSH treatment, blood sample was collected, and transabdominal scanning of the ovaries and uterus was performed. Twenty-four hours after the last FSH injection, the gilts were slaughtered and their ovaries and uterus were processed for histological and histomorphometric analysis. The histomorphometric parameters of the uterus differed (<em>P</em> &lt; 0.05) between prepubertal gilts at 160 d and 140 d of age. Moreover, changes (<em>P</em> &lt; 0.05) in uterine and ovarian ultrasound images occurred between 140 and 160 d of age. Age and FSH treatment did not affect (<em>P &gt;</em> 0.05) E2 plasma concentrations. Follicle stimulating hormone treatment did not affect (<em>P</em> &gt; 0.05) the early stage of folliculogenesis in the prepubertal gilts; however, the number of early atretic follicles decreased (<em>P</em> &lt; 0.05) after the FSH treatment. Follicle stimulating hormone administration increased (<em>P</em> &lt; 0.05) the number of medium follicles and decreased (<em>P</em> &lt; 0.05) the number of small follicles in 140 and 160 d old gilts. In the endometrium, luminal/glandular epithelium height and glandular diameter increased (<em>P</em> &lt; 0.05) after FSH treatment. Thus, injections of 100 mg of FSH stimulate the endometrium epithelium and induce follicular growth to a medium follicle size without affecting the preantral stages in prepubertal gilts; also, the uterine macroscopic morphometry does not change from 140 to 160 d of age.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106786"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9577686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between plasma dopamine concentration and temperament in horses","authors":"J. Kim ,&nbsp;H. Jung ,&nbsp;M. Yoon","doi":"10.1016/j.domaniend.2023.106788","DOIUrl":"10.1016/j.domaniend.2023.106788","url":null,"abstract":"<div><p>Dopamine (DA) is a neurotransmitter associated with animal behaviors. Along with other neurotransmitters such as oxytocin (OXT) and serotonin (5-HT), DA is also involved in determining the temperament of animals. However, the involvement of DA in horse temperament has not been well elucidated. Therefore, in this study, we aimed to determine the correlation between plasma DA concentration and OXT and 5-HT concentrations and behavioral temperament (eg, docility and friendliness, fearfulness, dominance, and trainability) of horses. Blood samples were collected from 31 horses and the concentrations of DA, OXT, and 5-HT were measured using enzyme-linked immunosorbent assay. The temperament of horses was assessed and scored by 3 researchers. The correlation between the plasma concentration of DA and OXT or 5-HT was statistically analyzed using SPSS software and linear regression analysis was performed to determine the association between DA concentration and OXT and 5-HT concentrations. Meanwhile, the DA concentration associated with each type of temperament was analyzed via one-way analysis of variance with LSD <em>post hoc</em> analysis as well as Student's <em>t</em>-test (for trainability). Plasma DA concentration was not found to be correlated with either OXT or 5-HT concentrations. Furthermore, we found no correlation between plasma DA concentration and dominance and trainability. However, our results suggest the possibility of predicting the degree of fearfulness of horses using plasma DA concentrations. We conclude that plasma DA concentration has a potentiality to be used as a biomarker to predict the fearfulness of horses.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106788"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9524566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}