Domestic animal endocrinology最新文献

{"title":"Association between post-ACTH cortisol and trilostane dosage in dogs with pituitary-dependent hypercortisolism","authors":"Fernanda Nastri Gouvêa ,&nbsp;Alessandra Martins Vargas ,&nbsp;Ednaldo Carvalho Guimarães ,&nbsp;Leandro Zuccolotto Crivellenti ,&nbsp;Caio Santos Pennacchi ,&nbsp;Hévila Dutra Barbosa de Cerqueira ,&nbsp;Luana de Oliveira Branco ,&nbsp;Natani Silva Reis ,&nbsp;Sofia Borin-Crivellenti","doi":"10.1016/j.domaniend.2024.106871","DOIUrl":"10.1016/j.domaniend.2024.106871","url":null,"abstract":"<div><p>Trilostane is the current treatment of choice for managing pituitary-dependent hypercortisolism (PDH) in dogs. While prescribing higher initial doses may elevate the risk of iatrogenic hypocortisolism, opting for more conservative approach could result in delayed disease control, since most individuals end up requiring dosage increases. The adrenocorticotrophin stimulation test (ACTHst), a widely recognized hormonal test for assessing adrenal function, is an essential tool for monitoring the pharmacological treatment of canine hypercortisolism (CH) that can also be used for diagnostic purposes. The aim of this study was to investigate the relationship between post-ACTH cortisol (cpACTH) at PDH diagnosis and the required trilostane dose for sign control and endogenous cortisol regulation in dogs, considering a hypothesis that higher serum cpACTH concentration would necessitate a higher trilostane dosage for disease management. Data for 43 dogs with PDH had their diagnostic cpACTH recorded and correlated to the trilostane dosage necessary to control clinical signs and achieve satisfactory cortisol levels (ideally 2-7 μg/dL). The odds ratio (p=0.042) suggests that dogs with cpACTH ≥ 27 μg/dL at diagnosis are 96% more likely to need a higher trilostane dosage for achieving satisfactory control of PDH. Thus, cpACTH was found to be associated with the final trilostane dose for controlling PDH in dogs.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106871"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141710070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocrine fibroblast growth factors in domestic animals","authors":"Emily J. Brinker ,&nbsp;Michael R. Hardcastle ,&nbsp;Keren E. Dittmer ,&nbsp;Emily C. Graff","doi":"10.1016/j.domaniend.2024.106872","DOIUrl":"10.1016/j.domaniend.2024.106872","url":null,"abstract":"<div><p>Fibroblast growth factors (FGFs) are a group of structurally homologous yet functionally pleiotropic proteins. Canonical and intracellular FGFs have primarily autocrine or paracrine effects. However, the FGF19 subfamily, composed of FGF15/19, FGF21, and FGF23, act as endocrine hormones that regulate bile acid, metabolic, and phosphorus homeostasis, respectively. Current research in human and rodent models demonstrates the potential of these endocrine FGFs to target various diseases, including disorders of inherited hypophosphatemia, chronic liver disease, obesity, and insulin resistance. Many diseases targeted for therapeutic use in humans have pathophysiological overlaps in domestic animals. Despite the potential clinical and economic impact, little is known about endocrine FGFs and their signaling pathways in major domestic animal species compared with humans and laboratory animals. This review aims to describe the physiology of these endocrine FGFs, discuss their current therapeutic use, and summarize the contemporary literature regarding endocrine FGFs in domestic animals, focusing on potential future directions.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106872"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0739724024000353/pdfft?md5=95b4cc62b1e98552fed88c7c5d9e54c4&pid=1-s2.0-S0739724024000353-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141717001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is it possible to replace eCG by GnRH in the hormonal protocol for timed artificial insemination in ewes during the breeding season?","authors":"Gabriel Maggi ,&nbsp;Otávio S. Pires ,&nbsp;Sérgio F. Vargas Junior ,&nbsp;Fernando C. Oliveira ,&nbsp;Fabiane P. Moraes ,&nbsp;Rogério Ferreira ,&nbsp;Arnaldo D. Vieira ,&nbsp;Monique T. Rovani ,&nbsp;Paulo B.D. Gonçalves ,&nbsp;Rafael G. Mondadori ,&nbsp;Bernardo G. Gasperin","doi":"10.1016/j.domaniend.2024.106878","DOIUrl":"10.1016/j.domaniend.2024.106878","url":null,"abstract":"<div><p>Hormonal protocols based on progestogens and equine chorionic gonadotrophin (eCG) are efficient for estrus and ovulation synchronization in ewes. Although eCG is indispensable during seasonal anestrus, it may not be necessary during the breeding season. Thus, we tested the hypothesis that GnRH is effective in replacing eCG during the breeding season allowing satisfactory ovulation rate, luteal function and conception rates after timed artificial insemination (TAI). Ewes (<em>n</em> = 134) with a minimum body condition score of 2.5 (0–5 scale) were treated with intravaginal devices (IVD) containing 60 mg of medroxyprogesterone acetate (MPA) for seven days and received 0.26 mg of sodium cloprostenol at the time of IVD removal. In Exp. 1, at IVD removal, ewes (<em>n</em> = 29) were allocated to three groups: eCG (200 IU at IVD removal; <em>n</em> = 10); eCG+GnRH (200 IU eCG at IVD removal and 4 µg of buserelin 36 h later; <em>n</em> = 10); or GnRH (buserelin 36 h after IVD removal; <em>n</em> = 9). Blood samples were collected 2, 6 and 12 days after TAI moment (54 h after IVD removal), for progesterone (P4) analysis. In Exp 2, the ewes were allocated to eCG (<em>n</em> = 10) or GnRH (<em>n</em> = 10) groups, as above described, and ovulation moment was evaluated 54, 66 and 78 h after IVD removal. In Exp 3, TAI was performed in ewes from eCG (<em>n</em> = 45) and GnRH (<em>n</em> = 40) groups using 100 × 10⁶ motile spermatozoa from a pool of semen collected from four rams. In Exp. 1, based on P4 levels, we confirmed that all the ewes ovulated (29/29) and there was no significant effect of group (<em>P</em> = 0.89) or group x day (<em>P</em> = 0.18) on P4 concentration, being observed a significant effect of day (<em>P</em> = 0.0001). In Exp. 2, the maximum DF diameter (<em>P</em> = 0.26) and ovulation moment (<em>P</em> = 0.69) did not differ between groups. In Exp. 3, pregnancy rate was significantly lower (<em>P</em> = 0.02) in GnRH (22.5 %; 9/40) compared to eCG (46.7 %; 21/45). The results indicate that, although ovulation and luteal function were not altered after eCG, eCG+GnRH or GnRH treatment, GnRH alone before TAI cannot be used to replace eCG treatment during the breeding season.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106878"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141990865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic inflammation decreases arcuate kisspeptin expression in male sheep","authors":"AN Renwick ,&nbsp;BK Whitlock ,&nbsp;CC Nestor ,&nbsp;JA Daniel ,&nbsp;L Strickland ,&nbsp;AS Lear ,&nbsp;M Adkins ,&nbsp;C Griffin ,&nbsp;A Esteller-Vico","doi":"10.1016/j.domaniend.2024.106868","DOIUrl":"10.1016/j.domaniend.2024.106868","url":null,"abstract":"<div><p>Lipopolysaccharide (LPS) from Gram-negative bacteria induces an immune response and impairs reproduction through suppression of gonadotropin releasing hormone (GnRH), subsequently luteinizing hormone (LH) secretion. While there is evidence that acute inflammation inhibits kisspeptin, little is known about the impact of chronic inflammation on this key reproductive neuropeptide in livestock species. Thus, we sought to examine a central mechanism whereby LPS suppresses LH secretion in sheep. Twenty wethers were randomly assigned to one of five treatment groups: control (CON; n=4), single acute IV LPS dose (SAD; n=4), daily acute IV LPS dose (DAD; n=4), daily increasing IV LPS dose (DID; n=4), and chronic subcutaneous LPS dose (CSD; n=4). On Days 1 and 7, blood samples were collected every 12 minutes for 360 minutes using jugular venipuncture. Following blood collection on Day 7, all animals were euthanized, brain tissue was perfused with 4% paraformaldehyde, and hypothalamic blocks were removed and processed for immunohistochemistry. On Day 1, LH pulse frequency was significantly lower (p=0.02) in SAD (0.25 ± 0.1 pulses/hour), DAD (0.25 ± 0.1 pulses/hour), DID (0.35 ± 0.1 pulses/hour), and CSD (0.40 ± 0.1 pulses/hour) compared to CON (0.70 ±0.1 pulses/hour). On Day 7, only DID animals (0.35 ± 0.1 pulses/hour) had significantly lower (p=0.049) LH pulse frequency compared to controls (0.85 ± 0.1 pulse/hour). Furthermore, only DID animals (33.3 ± 10.9 cells/section/animal) had significantly fewer (p=0.001) kisspeptin-immunopositive cells compared to controls (82.6 ± 13.6 cells/section/animal). Taken together, we suggest that daily increasing doses of LPS is a powerful inhibitor of kisspeptin neurons in young male sheep and a physiologically relevant model to examine the impact of chronic inflammation on the reproductive axis in livestock.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106868"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141414968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal variation of NGF in seminal plasma and expression of NGF and its cognate receptors NTRK1 and p75NTR in the sex organs of rams","authors":"Francesca Mercati ,&nbsp;Gabriella Guelfi ,&nbsp;José Ignacio Martí ,&nbsp;Cecilia Dall'Aglio ,&nbsp;Lucía Calleja ,&nbsp;Domenico Caivano ,&nbsp;Maria Luisa Marenzoni ,&nbsp;Camilla Capaccia ,&nbsp;Polina Anipchenko ,&nbsp;Francesco Alessandro Palermo ,&nbsp;Paolo Cocci ,&nbsp;Mario Rende ,&nbsp;Massimo Zerani ,&nbsp;Margherita Maranesi","doi":"10.1016/j.domaniend.2024.106877","DOIUrl":"10.1016/j.domaniend.2024.106877","url":null,"abstract":"<div><p>Nerve growth factor (NGF) has long been known as the main ovulation-inducing factor in induced ovulation species, however, recent studies suggested the NGF role also in those with spontaneous ovulation. The first aim of this study was to evaluate the presence and gene expression of NGF and its cognate receptors, high-affinity neurotrophic tyrosine kinase 1 receptor (NTRK1) and low-affinity p75 nerve growth factor receptor (p75NTR), in the ram genital tract. Moreover, the annual trend of NGF seminal plasma values was investigated to evaluate the possible relationship between the NGF production variations and the ram reproductive seasonality. The presence and expression of the NGF/receptors system was evaluated in the testis, epididymis, vas deferens ampullae, seminal vesicles, prostate, and bulbourethral glands through immunohistochemistry and real-time PCR (qPCR), respectively. Genital tract samples were collected from 5 adult rams, regularly slaughtered at a local abattoir. Semen was collected during the whole year weekly, from 5 different adult rams, reared in a breeding facility, with an artificial vagina. NGF seminal plasma values were assessed through the ELISA method. NGF, NTRK1 and p75NTR immunoreactivity was detected in all male organs examined. NGF-positive immunostaining was observed in the spermatozoa of the germinal epithelium, in the epididymis and the cells of the secretory epithelium of annexed glands, NTRK1 receptor showed a localization pattern like that of NGF, whereas p75NTR immunopositivity was localized in the nerve fibers and ganglia. NGF gene transcript was highest (<em>p &lt;</em> 0.01) in the seminal vesicles and lowest (<em>p &lt;</em> 0.01) in the testis than in the other tissues. NTRK1 gene transcript was highest (<em>p &lt;</em> 0.01) in the seminal vesicles and lowest (<em>p &lt;</em> 0.05) in all the other tissues examined. Gene expression of p75NTR was highest (<em>p &lt;</em> 0.01) in the seminal vesicles and lowest (<em>p &lt;</em> 0.01) in the testis and bulbourethral glands. NGF seminal plasma concentration was greater from January to May (<em>p &lt;</em> 0.01) than in the other months. This study highlighted that the NGF system was expressed in the tissues of all the different genital tracts examined, confirming the role of NGF in ram reproduction. Sheep are short-day breeders, with an anestrus that corresponds to the highest seminal plasma NGF levels, thus suggesting the intriguing idea that this factor could participate in an inhibitory mechanism of male reproductive activity, activated during the female anestrus.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106877"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0739724024000407/pdfft?md5=cc2af91e236290d3af10d9ffa9be3226&pid=1-s2.0-S0739724024000407-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incretin therapy in feline diabetes mellitus – A review of the current state of research","authors":"Nina Haller ,&nbsp;Thomas A. Lutz","doi":"10.1016/j.domaniend.2024.106869","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106869","url":null,"abstract":"<div><p>Incretin hormones potentiate the glucose-induced insulin secretion following enteral nutrient intake. The best characterised incretin hormones are glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) which are produced in and secreted from the gut in response to nutrient ingestion. The property of incretins to enhance endogenous insulin secretion only at elevated blood glucose levels makes them interesting therapeutics for type 2 diabetes mellitus with a better safety profile than exogenous insulin. While incretin therapeutics (especially GLP-1 agonists, and more recently also GLP-1 / GIP dual agonists and other drugs that influence the incretin metabolism (e.g., dipeptidyl peptidase-4 (DPP-4) inhibitors)) are already widely used treatment options for human type 2 diabetes, these drugs are not yet approved for the therapy of feline diabetes mellitus. This review provides an introduction to incretins and feline diabetes mellitus in general and summarises the current study situation on incretins as therapeutics for feline diabetes mellitus to assess their possible future potential in feline medicine. Studies to date on the use of GLP-1 receptor agonists (GLP-1RA) in healthy cats largely confirm their insulinotropic effect known from other species. In diabetic cats, GLP-1RAs appear to significantly reduce glycaemic variability (GV, an indicator for the quality of glycaemic control), which is important for the management of the disease and prevention of long-term complications. However, for widespread use in feline diabetes mellitus, further studies are required that include larger numbers of diabetic cats, and that consider and test a possible need for dose adjustments to overweight and diabetic cats. Also evaluation of the outcome of GLP-1RA monotherapy will be neceessary.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106869"},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141313796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin interacts with PPARγ agonists to promote bovine adipocyte differentiation","authors":"Pan−Pan Guo ,&nbsp;Xue−Rui Yao ,&nbsp;Yong−Nan Xu ,&nbsp;Xin Jin ,&nbsp;Qiang Li ,&nbsp;Chang−Guo Yan ,&nbsp;Nam−Hyung Kim ,&nbsp;Xiang−Zi Li","doi":"10.1016/j.domaniend.2024.106848","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106848","url":null,"abstract":"<div><p>Insulin is a potent adipogenic hormone that triggers a series of transcription factors that regulate the differentiation of preadipocytes into mature adipocytes. Ciglitazone specifically binds to peroxisome proliferator−activated receptor−<em>γ</em> (<em>PPARγ</em>), thereby promoting adipocyte differentiation. As a natural ligand of <em>PPARγ</em>, oleic acid (OA) can promote the translocation of <em>PPARγ</em> into the nucleus, regulate the expression of downstream genes, and promote adipocyte differentiation. We hypothesized that ciglitazone and oleic acid interact with insulin to enhance bovine preadipocyte differentiation. Preadipocytes were cultured 96 h in differentiation medium containing 10 mg/L insulin (I), 10 mg/L insulin + 10 µM cycloglitazone (IC), 10 mg/L insulin + 100 µM oleic acid (IO), or 10 mg/L insulin + 10 µM cycloglitazone+100 µM oleic acid (ICO). Control preadipocytes (CON) were cultured in differentiation medium (containing 5% fetal calf serum). The effects on the differentiation of Yanbian cattle preadipocytes were examined using molecular and transcriptomic techniques, including differentially expressed genes (DEGs) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis. I, IC, IO, and ICO treatments produced higher concentrations of triglycerides (TAG) and lipid droplet accumulation in preadipocytes compared with CON treatment (<em>P</em> &lt; 0.05). Co−treatment of insulin and <em>PPARγ</em> agonists significantly increased the expression of genes involved in regulating adipogenesis and fatty acid synthesis. (<em>P</em> &lt; 0.05). Differential expression analysis identified 1488, 1764, 1974 and 1368 DEGs in the I, IC, IO and ICO groups, respectively. KEGG pathway analysis revealed DEGs mainly enriched in <em>PPAR</em> signalling, <em>FOXO</em> signaling pathway and fatty acid metabolism. These results indicate that OA, as <em>PPARγ</em> agonist, can more effectively promote the expression of bovine lipogenesis genes and the content of TAG and adiponectin when working together with insulin, and stimulate the differentiation of bovine preadipocytes. These findings provide a basis for further screening of relevant genes and transcription factors in intramuscular fat deposition and meat quality to enhance breeding programs.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106848"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140341348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-specific analysis of anti-müllerian hormone and liver biochemical parameters in freemartin holstein calves and heifers: A pilot study","authors":"Gokhan Bozkurt ,&nbsp;Feyyaz Kaya ,&nbsp;Mehmet Yildiz ,&nbsp;Sultan Sengul ,&nbsp;Ismail Ilker Kocaer ,&nbsp;Halil Erkan Sarıbas ,&nbsp;Orsan Gungor ,&nbsp;Ibrahim Tasal ,&nbsp;Yigit Can Aslan ,&nbsp;Serdar Dursun Ak ,&nbsp;Afsin Koker","doi":"10.1016/j.domaniend.2024.106855","DOIUrl":"10.1016/j.domaniend.2024.106855","url":null,"abstract":"<div><p>Freemartinism is the most common congenital anomaly among sexual disorders in dairy cows. This syndrome typically occurs in different-sex twin pregnancies and causes vascular anastomoses to form with the placenta in the early stages of fetal development. The study aims to determine the effectiveness of Anti-müllerian hormone (AMH) levels in calves and heifers of different age groups for diagnostic factors and to investigate the potential consequences of different hormone levels in different age groups on some liver biochemical parameters. The study involved 50 cattle from diverse age categories, divided into the freemartin group (FM Group, n=25) and the control group (C Group, n=25). Both FM and control groups were further divided into early-age (3-5 months), middle-aged (5-9 months), and older-aged groups (9-12 months). Serum AMH levels, along with total protein, albumin, and total cholesterol levels, were measured. While no statistically significant difference in AMH levels was observed in the early-age group (P:0.53), significant differences were determined in the middle (P:0.015) and older-age groups (P:0.01), where the FM group exhibited significantly decreased AMH levels compared to the control group. The evaluation of liver biochemistry revealed a statistically significant difference in total protein levels between the FM and control groups in the older age group (P:0.033). Consequently, it is reasonable to suggest that AMH levels may serve as a valid parameter for diagnosing freemartin syndrome in calves aged older than five months. Conversely, particularly in young calves, no significant differences in liver functionality were observed between freemartin-affected and healthy calves.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106855"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141039973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prostaglandin F2α treatment does not hasten ovulation in weaned sows","authors":"D.P. Fagundes ,&nbsp;M.S. Lucca ,&nbsp;B.G. Gasperin ,&nbsp;D. Missio ,&nbsp;M. Quirino ,&nbsp;A.P.G. Mellagi ,&nbsp;P.B.D. Gonçalves ,&nbsp;F.P. Bortolozzo ,&nbsp;R.R. Ulguim","doi":"10.1016/j.domaniend.2024.106854","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106854","url":null,"abstract":"<div><p>This study evaluated the efficiency of prostaglandin F2α (PGF) to hasten ovulation in weaned sows. In experiment I, weaned sows detected in estrus (0 h) received: no hormone (Control; <em>n</em> = 56); 0.5 mg PGF IM at 0 h and 2 h (PGF0; <em>n</em> = 56); or 0.5 mg PGF IM at 24 h and 26 h (PGF24; <em>n</em> = 55). In experiment II, weaned sows that did not express estrus signs until 72 h after weaning (0 h) were assigned to: no hormone (Control; <em>n</em> = 45); 10 µg buserelin acetate IM at 0 h (Buserelin; <em>n</em> = 43); 0.5 mg PGF IM at 34 h and 36 h (PGF; <em>n</em> = 44); or 10 µg buserelin acetate IM at 0 h plus 0.5 mg PGF IM at 34 h and 36 h (Buserelin + PGF; <em>n</em> = 45). In experiment I, no effect of PGF on the interval treatment onset to ovulation was observed (<em>P</em> &gt; 0.05), and no treatment effect was observed on the relative or cumulative proportion of females that ovulated post-treatment onset (<em>P</em> &gt; 0.05). In experiment II, treatment onset to ovulation interval was shorter for Buserelin group than for PGF group (<em>P</em> &lt; 0.05), and a higher cumulative percentage of Buserelin treated sows ovulated up to 48 h compared to PGF and Control groups (<em>P</em> &lt; 0.01), with no differences from Buserelin + PGF. Treatments did not affect total number of piglets born in both experiments (<em>P</em> &gt; 0.05). In conclusion, PGF did not hasten ovulation timing or affect litter size in weaned sows.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106854"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early induction of luteolysis in a timed AI protocol increases reproductive performance in beef cows","authors":"Eduardo P. Silva ,&nbsp;Alfredo Q. Antoniazzi ,&nbsp;João B.S. Borges ,&nbsp;Pedro L.J. Monteiro ,&nbsp;Amanda B. Machado ,&nbsp;Marcelo M. Dias ,&nbsp;Valério V.M. Portela Junior ,&nbsp;Milo C. Wiltbank","doi":"10.1016/j.domaniend.2024.106853","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106853","url":null,"abstract":"<div><p>The aim of this study was to produce a longer proestrus by early administration of prostaglandin F2α (PGF) in a timed artificial insemination (TAI) protocol in non-suckling <em>Bos taurus</em> (Angus crossbreed) beef cows. On day 0, cows (n = 489) were treated with an intravaginal 1 g progesterone (P4) device and 2 mg of estradiol benzoate. On day 7, cows were randomized into two groups: PGF7(n = 244; 500 µg of sodium cloprostenol 24 h before P4 device removal) or PFG8 (n = 245; 500 µg of sodium cloprostenol at P4 device removal). On day 8, P4 device was removed and cows received 0.5 mg of estradiol cypionate. All cows were submitted to TAI on day 10 (48–50 hours after P4 device removal). Cows treated with PGF on day 7 had greater expression of estrus (91.3 vs 79.1 %; P = 0.0011), regardless of CL presence at beginning of the protocol. Cows from PGF7 group had lower circulating P4 concentrations on day 8 in comparison with PGF8 treated cows (1.86 vs 2.99 ng/mL; P &lt; 0.001). However, preovulatory follicle diameter did not differ among treatments at TAI (11.9 vs 11.8 mm; P = 0.7881). Pregnancy per TAI (P/TAI) was greater for PGF7 (63.9 vs 50.6 %; P = 0.0114) than PGF8 treated cows. In cows with follicles &lt;8.5 mm at TAI, expression of estrus (33.3 vs 26.6 %; P = 0.6427) and P/TAI (40 vs 26.6 %; P = 0.3657) were low in both PGF7 and PGF8 treated cows, respectively. In cows with medium follicle size (8.5 to 11.9 mm) PGF7 treated cows had greater expression of estrus (90.5 vs 80 %; P = 0.033) and P/TAI (62.2 vs 49 %; P = 0.053). In cows with follicles &gt;12 mm, expression of estrus was greater for PGF7 than PGF8 treated cows (99.1 vs 93.3 %; P = 0.045), however P/TAI did not differ (68.2 vs 59 %; P = 0.149). In cows with P4 &lt; 1.99 ng/mL on day 8, expression of estrus was similar between PGF7 and PGF8 treated cows (92.6 vs 90.4 %; P = 0.53), and P/TAI tended to be greater for PGF7 than PGF8 treated cows (63 vs 52.1 % P = 0.076). However, in cows with P4 &gt; 2 ng/mL PGF7 cows had higher expression of estrus (89 vs 67.5 %; P = 0.0005) and P/TAI (64.8 vs 48.7 %; P = 0.021) than PGF8. Thus, increasing the proestrous period by inducing luteolysis 24 hours earlier than removing the P4 intravaginal device enhanced fertility in non-suckling cyclic beef cows by increasing expression of estrus and P/TAI.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106853"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}