Domestic animal endocrinology最新文献

{"title":"Developmental and hormonal regulation of FBN1 and OR4M1 mRNA in bovine granulosa cells","authors":"E.R.S. Maylem ,&nbsp;L.J. Spicer ,&nbsp;I.M. Batalha ,&nbsp;L.F. Schütz","doi":"10.1016/j.domaniend.2023.106791","DOIUrl":"10.1016/j.domaniend.2023.106791","url":null,"abstract":"<div><p>Recent studies have reported hormonal regulation of expression of <em>fibrillin 1</em> (<em>FBN1)</em>, the gene that encodes asprosin, in bovine theca cells, however, hormonal regulation of gene expression of <em>FBN1</em> and the asprosin receptor, <em>olfactory receptor 4M1</em> (<em>OR4M1),</em> has not been evaluated in granulosa cells (GC). This study was designed to characterize <em>FBN1</em> and <em>OR4M1</em> gene expression in GC during development of bovine dominant ovarian follicles, and to determine the hormonal regulation of <em>FBN1</em> and <em>OR4M1</em> mRNA expression in GC. GC <em>FBN1</em> mRNA abundance was greater <em>(P</em> &lt; 0.05) in medium (5.1–8 mm) estrogen inactive (EI) follicles than in large (&gt;8.1 mm) or small (1–5 mm) EI follicles. In comparison, GC <em>OR4M1</em> mRNA abundance was greater (<em>P</em> &lt; 0.05) in small EI follicles than in large or medium EI follicles. Abundance of <em>OR4M1</em> mRNA in GC of follicles collected on days 3 to 4 (early growth phase) and on days 5 to 6 (late growth phase) was similar, whereas <em>FBN1</em> mRNA abundance was greater (<em>P</em> &lt; 0.05) on days 5 to 6 vs days 3 to 4. Hormonal regulators for <em>FBN1</em> mRNA abundance in cultured small-follicle GC were identified: TGFβ1 causing a 2.45-fold increase, WNT3A causing a 1.45-fold increase, and IGF1 causing a 65% decrease. Steroids, leptin, insulin, growth hormone, follicle stimulating hormone, fibroblast growth factor 9 and epidermal growth factor had no effect on <em>FBN1</em> mRNA abundance. Abundance of <em>OR4M1</em> mRNA in GC was regulated by progesterone with 3.55-fold increase, but other hormones did not affect GC <em>OR4M1</em> mRNA abundance. Findings indicate that both <em>FBN1</em> and <em>OR4M1</em> gene expression are hormonally and developmentally regulated in bovine follicles, and thus may affect asprosin production and its subsequent role in ovarian follicular function in cattle.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"84 ","pages":"Article 106791"},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10029071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of FSH stimulation and age on the ovarian and uterine traits and histomorphometry of prepubertal gilts","authors":"V.A.P. Alfradique ,&nbsp;D.L. Souza Netto ,&nbsp;S.V.P. Alves ,&nbsp;A.F. Machado ,&nbsp;C.M. Novaes ,&nbsp;J.M. Penitente-Filho ,&nbsp;M. Machado-Neves ,&nbsp;M.S. Lopes ,&nbsp;S.E.F. Guimarães","doi":"10.1016/j.domaniend.2023.106786","DOIUrl":"10.1016/j.domaniend.2023.106786","url":null,"abstract":"<div><p>This study investigated the effect of age and follicle stimulating hormone (FSH) treatment on the estradiol (E2) plasma concentration, ovarian follicle development, endometrial histomorphometry, and ultrasonographic parameters of the ovaries and uterus in prepubertal gilts. Thirty-five prepubertal gilts were grouped according to age (140 or 160 d), and within each age, gilts were allotted to receive 100 mg of FSH (treated; G140 + FSH [n = 10] and G160 + FSH [n = 7]) or saline solution (control; G140 + control [n = 10] and G160 + control [n = 8]). The total dose of FSH was divided into 6 similar doses administered every 8 h (days 0–2). Before and after FSH treatment, blood sample was collected, and transabdominal scanning of the ovaries and uterus was performed. Twenty-four hours after the last FSH injection, the gilts were slaughtered and their ovaries and uterus were processed for histological and histomorphometric analysis. The histomorphometric parameters of the uterus differed (<em>P</em> &lt; 0.05) between prepubertal gilts at 160 d and 140 d of age. Moreover, changes (<em>P</em> &lt; 0.05) in uterine and ovarian ultrasound images occurred between 140 and 160 d of age. Age and FSH treatment did not affect (<em>P &gt;</em> 0.05) E2 plasma concentrations. Follicle stimulating hormone treatment did not affect (<em>P</em> &gt; 0.05) the early stage of folliculogenesis in the prepubertal gilts; however, the number of early atretic follicles decreased (<em>P</em> &lt; 0.05) after the FSH treatment. Follicle stimulating hormone administration increased (<em>P</em> &lt; 0.05) the number of medium follicles and decreased (<em>P</em> &lt; 0.05) the number of small follicles in 140 and 160 d old gilts. In the endometrium, luminal/glandular epithelium height and glandular diameter increased (<em>P</em> &lt; 0.05) after FSH treatment. Thus, injections of 100 mg of FSH stimulate the endometrium epithelium and induce follicular growth to a medium follicle size without affecting the preantral stages in prepubertal gilts; also, the uterine macroscopic morphometry does not change from 140 to 160 d of age.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106786"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9577686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between plasma dopamine concentration and temperament in horses","authors":"J. Kim ,&nbsp;H. Jung ,&nbsp;M. Yoon","doi":"10.1016/j.domaniend.2023.106788","DOIUrl":"10.1016/j.domaniend.2023.106788","url":null,"abstract":"<div><p>Dopamine (DA) is a neurotransmitter associated with animal behaviors. Along with other neurotransmitters such as oxytocin (OXT) and serotonin (5-HT), DA is also involved in determining the temperament of animals. However, the involvement of DA in horse temperament has not been well elucidated. Therefore, in this study, we aimed to determine the correlation between plasma DA concentration and OXT and 5-HT concentrations and behavioral temperament (eg, docility and friendliness, fearfulness, dominance, and trainability) of horses. Blood samples were collected from 31 horses and the concentrations of DA, OXT, and 5-HT were measured using enzyme-linked immunosorbent assay. The temperament of horses was assessed and scored by 3 researchers. The correlation between the plasma concentration of DA and OXT or 5-HT was statistically analyzed using SPSS software and linear regression analysis was performed to determine the association between DA concentration and OXT and 5-HT concentrations. Meanwhile, the DA concentration associated with each type of temperament was analyzed via one-way analysis of variance with LSD <em>post hoc</em> analysis as well as Student's <em>t</em>-test (for trainability). Plasma DA concentration was not found to be correlated with either OXT or 5-HT concentrations. Furthermore, we found no correlation between plasma DA concentration and dominance and trainability. However, our results suggest the possibility of predicting the degree of fearfulness of horses using plasma DA concentrations. We conclude that plasma DA concentration has a potentiality to be used as a biomarker to predict the fearfulness of horses.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106788"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9524566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal and immunological responses of Holstein dairy cows from late lactation to the dry period and from the dry period to early lactation","authors":"J. Gao ,&nbsp;T.N. Marins ,&nbsp;J.O.S. Calix ,&nbsp;Z. Qi ,&nbsp;J.K. Bernard ,&nbsp;S. Tao","doi":"10.1016/j.domaniend.2023.106790","DOIUrl":"10.1016/j.domaniend.2023.106790","url":null,"abstract":"<div><p>Parturition and dry-off are challenging events for dairy cows partially due to changes in endocrine responses. The aim of this experiment was to evaluate blood concentrations of cortisol and prolactin and their effects on proliferation of peripheral blood mononuclear cells (PBMC) with or without stimulation by common immune cell mitogens (lipopolysaccharide [LPS], and concanavalin A [ConA]) of multiparous dairy cows from late lactation to the dry period and from the dry period to early lactation. Two groups of cows were enrolled: cows from late lactation to the dry period enrolled at 8 d before dry-off (LTD, n = 6, days in milk at dry-off = 332 ± 41 d) and cows from the dry period to early lactation enrolled at 7 d before expected calving date (DTL, n = 7). Blood was collected on d -8, 3, 7, and 15 relatives to dry-off for LTD cows, and on d -7, 3, 7, and 21 relatives to calving for DTL cows to analyze circulating stress hormones and to isolate PBMC. The PBMC were stimulated in vitro with prolactin (PRL), hydrocortisone (HDC), LPS, ConA, PRL + LPS, PRL + ConA, HDC+LPS, and HDC + ConA to assess proliferative responses. Plasma cortisol and PRL concentrations of LTD and DTL cows were not affected by time. Regardless of time, addition of HDC reduced PBMC proliferation stimulated by LPS, but PRL had no effect. No time effect was observed for proliferation of PBMC collected from LTD cows, but PBMC collected at 21 d after calving had higher proliferative responses to LPS and ConA than those from late dry period or early lactation. In conclusion, results from this experiment confirmed the lower PBMC proliferation during the transition period from the final week of gestation to early lactation and suggested that cows transitioning from late lactation to dry period maintained unchanged cell-mediated immune function.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106790"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9527290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Irisin in domestic animals","authors":"E. Lai,&nbsp;S. Unniappan","doi":"10.1016/j.domaniend.2023.106787","DOIUrl":"10.1016/j.domaniend.2023.106787","url":null,"abstract":"<div><p>Irisin is a 112 amino acid peptide hormone cleaved from the fibronectin type III domain-containing protein. Irisin is highly conserved across vertebrates, suggesting evolutionarily conserved common functions among domestic animals. These functions include the browning of white adipose tissue and increased energy expenditure. Irisin has been detected and studied primarily in plasma, serum, and skeletal muscle, but has also been found in adipose tissue, liver, kidney, lungs, cerebrospinal fluid, breast milk, and saliva. This wider tissue presence of irisin suggests additional functions beyond its role as a myokine in regulating energy use. We are beginning to understand irisin in domestic animals. The goal of this review is to provide an up-to-date commentary on irisin structure, tissue distribution, and functions across vertebrates, especially mammals of importance in veterinary medicine. Irisin could be explored as a potential candidate for developing therapeutic agents and biomarkers in domestic animal endocrinology.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106787"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral administration of domperidone in the first or third week of lactation: Effects on prolactin concentrations and mammary gene expression in sows and piglet growth","authors":"C. Farmer,&nbsp;M.-F. Palin","doi":"10.1016/j.domaniend.2023.106789","DOIUrl":"10.1016/j.domaniend.2023.106789","url":null,"abstract":"<div><p>The scope of the present study is endocrine and metabolic control of sow lactation. This project aimed to determine the impact of increasing prolactin concentrations via oral administration of the dopamine receptor antagonist domperidone in the first or third week of lactation in sows. Effects on sow hormonal and metabolic status, lactational performance, and gene expression in mammary epithelial cells were determined. Primiparous sows were divided in 3 treatments: 1) 10 mL of vehicle (table syrup) per os twice daily during the first and third weeks of lactation (Control, CTL, n = 23), 2) 0.5 mg/kg of domperidone per os twice daily during the first week of lactation (LACT1, n = 23), or 3) 0.5 mg/kg of domperidone given per os twice daily during the third week of lactation (LACT3, n = 22). Treated sows also received 10 mL of the vehicle twice daily during the other treatment period. Litter size was standardized to 12 ± 1 and piglets were weighed at birth, 24 h, and on d 8, 15, 22 (weaning), 35, and 56. Sow feed intake was recorded daily. Representative milk samples were obtained on d 7 and 21 of lactation for compositional analyses, and milk fat globules were used to measure mRNA abundances of various genes. Jugular blood samples were obtained from sows on d 1, 7, 14, and 21 of lactation to measure concentrations of prolactin, IGF-1, insulin, urea, and FFA. Concentrations of prolactin were increased (<em>P</em> &lt; 0.01) at the end of the 7-d treatment period with domperidone, whether imposed in the first (LACT1) or third (LACT 3) week of lactation. No other blood variables were affected by treatments and neither was milk composition (<em>P</em> &gt; 0.10). Sow BW, backfat thickness, or feed intake were not altered by treatments (<em>P</em> &gt; 0.10), but piglet BW tended to be greater in litters from LACT3 compared with CTL sows on d 22 and 35 (<em>P</em> ≤ 0.10). Gene expression of <em>EGF</em> in milk fat globules tended to be (LACT1, <em>P</em> &lt; 0.10) or was increased (LACT3, <em>P</em> &lt; 0.05) after treatment, and the effect in LACT1 sows was maintained until d 21 of lactation. The mRNA abundance of <em>SPP1</em> was increased (<em>P</em> &lt; 0.05) in LACT1 vs CTL sows on d 7, and that of 3 major milk proteins tended to be (<em>CSN1S2</em> and <em>WAP, P &lt; 0.10)</em> or was greater (<em>LALBA, P</em> &lt; 0.05) in LACT3 vs CTL sows on d 21 of lactation. Oral administration of domperidone during the first or third week of lactation increased prolactin concentrations and altered mRNA abundances of selected genes in milk fat globules. Yet, only the LACT 3 treatment positively affected piglet performance.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106789"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9529769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of environmental and nutritional stresses on milk fat synthesis in dairy cows","authors":"A. Razzaghi ,&nbsp;M.H. Ghaffari ,&nbsp;D.E. Rico","doi":"10.1016/j.domaniend.2022.106784","DOIUrl":"10.1016/j.domaniend.2022.106784","url":null,"abstract":"<div><p>Stress reduces milk and milk components synthesis and increases maintenance requirements of cows. The major stress-related alterations involve enhanced secretion of glucocorticoids and increased sympathetic nervous system activity, which results in biochemical and physiologic changes. In dairy cows exposed to social (ie housing conditions, overstocking, regrouping, feed delivery), physiological (ie initiation of lactation and parturition), or physical (ie heat or cold stress) stressors, responses involve alterations in energy balance and nutrient partitioning. The capacity of the animal to synthesize milk fat largely depends on the availability of substrates for lipid synthesis from the diet, ruminal fermentation or adipose tissue stores, all of which can be altered under stress conditions. Indeed, milk fat concentration is particularly responsive to diet and environment modifications, where a wide range of nutritional and non-nutritional factors influence milk fat output. Milk fat synthesis is an energy demanding process, and extremely sensitive to stress factors during lactation and the involvement of multiple organs. Recent studies examining social, physical, and physiological stressors have provided important insights into how differences in milk yield and milk components may be associated with biological responses to stress factors in dairy cows. This review focuses primarily on the role of stress sources and indicators to which the dairy cow is exposed in regulating milk fat synthesis. We will review the role of nutritional and non-nutritional factors on milk fat synthesis in dairy cows under stress conditions.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106784"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9529758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short Communication: Identification of equine corticotropin-like intermediate lobe peptide (CLIP) binding to an adrenocortipcotrophic hormone (ACTH) assay capture antibody","authors":"E.J. Knowles ,&nbsp;C. Hyde ,&nbsp;P.A. Harris ,&nbsp;J. Elliott ,&nbsp;N.J. Menzies-Gow","doi":"10.1016/j.domaniend.2023.106785","DOIUrl":"10.1016/j.domaniend.2023.106785","url":null,"abstract":"<div><p>A chemiluminescent immunoassay is commonly employed to measure adrenocorticotrophic hormone (ACTH) concentrations to assist pituitary <em>pars intermedia</em> dysfunction diagnosis. In a previous study, seasonally-dependent assay cross-reactivity to endogenous equine corticotropin-like intermediate lobe peptide (CLIP, ACTH 18–39) was suspected. The present study aimed to demonstrate binding of endogenous equine CLIP to the capture antibody of the ACTH chemiluminescent immunoassay. Liquid chromatography – mass spectrometry (LCMS) methods were optimised to identify selected ions from synthetic human ACTH, α-melanocyte stimulating hormone (α-MSH, ACTH 1–17) and CLIP. Synthetic ACTH and CLIP bound to the capture antibody of the chemiluminescent ACTH assay, but α-MSH did not. Equine endogenous CLIP was detected by LCMS in pony plasma taken in the autumn and could be eluted from the capture antibody of the ACTH chemiluminescent immunoassay. Further research is required to enable quantification of CLIP. Equine CLIP may alter measured ACTH concentrations <em>in vivo.</em></p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"83 ","pages":"Article 106785"},"PeriodicalIF":2.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9517773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes","authors":"J.H. Dias ,&nbsp;J.D. Gonçalves ,&nbsp;A.M. Arrais ,&nbsp;J.M.G. Souza-Fabjan ,&nbsp;R. Bastos ,&nbsp;R.I.T.P. Batista ,&nbsp;L.G.B. Siqueira ,&nbsp;M.E.F. Oliveira ,&nbsp;J.F. Fonseca","doi":"10.1016/j.domaniend.2022.106751","DOIUrl":"10.1016/j.domaniend.2022.106751","url":null,"abstract":"<div><p>This study investigated the effectiveness of different doses of estradiol benzoate (EB) to promote cervical relaxation and their effects on luteal function and outcomes of non-surgical embryo recovery (NSER) in sheep. Multiparous (MULT) and nulliparous (NULL) crossbred Lacaune X Santa Inês ewes were superovulated and naturally bred. Seven days after progesterone withdrawal, females were randomly assigned to one of three distinct cervical relaxation protocols, consisting of i.m. treatment with 37.5 µg d-cloprostenol and different doses of EB: 0.0 mg (0.0EB group; n = 3 NULL and 14 MULT); 0.5 mg (0.5EB group; n = 4 NULL and 12 MULT) or 1.0 mg (1.0EB group, n = 6 NULL and 11 MULT) 16 h before NSER. All ewes received 50 IU of oxytocin 20 min before NSER (D17). Blood samples were collected and ultrasound exams (B-mode and color Doppler) were performed at two timepoints: immediately before d-cloprostenol and EB treatments and prior to NSER. Estrous behavior, corpora lutea count and NSER success outcomes were not affected by EB treatments nor parity (<em>P</em> &gt; 0.05). Embryo recovery rate was greater for ewes in the 0.5EB group and in the NULL ewes (<em>P</em> &lt; 0.05). Ovarian biometrics differed between the two evaluation timepoints in all groups (<em>P</em> &lt; 0.05). Plasma estradiol increased over time, reaching a significant greater level in 1.0EB ewes compared to controls on D17 (<em>P</em> &lt; 0.05), whereas progesterone concentrations decreased over time in all groups (<em>P</em> &gt; 0.05). In conclusion, treatments did not affect NSER success but they did affect luteal function by altering P4 and E2 concentrations. Therefore, the NSER technique can be successfully performed in ewes with or without prior treatment with EB.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"82 ","pages":"Article 106751"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40555051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a single dose of long-acting FSH on testicular blood flow, testicular echotexture, and circulating testosterone, estradiol, and nitric oxide in rams during the non-breeding season","authors":"H. Samir ,&nbsp;A.S. El-Shalofy,&nbsp;H.R. El-Sherbiny","doi":"10.1016/j.domaniend.2022.106765","DOIUrl":"10.1016/j.domaniend.2022.106765","url":null,"abstract":"<div><p>The present study investigated the effect of a single administration of long-acting follicle simulation hormone (FSH) on testicular blood perfusion as measured by pulsed-wave Doppler ultrasonography, testicular echotexture, and circulating testosterone (T), estradiol (E2), and nitric oxide (NO) in the plasma of rams in the non-breeding season. Twelve Ossimi rams were subjected to either a single administration of long-acting FSH subcutaneously (FSH group; n = 6) or the vehicle (control group; n = 6). Assessment of testicular hemodynamics at the level of the supratesticular artery was performed just before administration (0 h), and at 4, 24, 48, 72, 96, and 168 h after FSH or the vehicle administrations. Testicular volume (TV), and echotexture of testicular parenchyma including pixel intensity and heterogeneity were derived by the computer analysis software. Concentrations of T, E2, and NO were measured using commercial kits. Results revealed significant decreases (<em>P</em> ˂ 0.05) in the values of Doppler indices (resistive index: RI and pulsatility index: PI), especially at 48 h after administration of FSH (RI: 0.42 ± 0.02, PI: 0.56 ± 0.04) compared to their values in the control group (RI: 0.54 ± 0.03, PI: 0.77 ± 0.04). FSH administration induced significant decreases (<em>P</em> ˂ 0.05) in the pixel intensity of testicular parenchyma. Testicular volume and T concentrations were not significantly changed (<em>P</em> ˃ 0.05). Concentrations of E2 increased significantly (<em>P</em> ˂ 0.05) at 48 h and 72 h after FSH administration. (30.07 ± 5.23 pg/ml, 29.93 ± 1.44 pg/ml, respectively) compared to their values before FSH administration (14.63 ± 1.37 pg/ml). Concentrations of NO increased significantly (<em>P</em> ˂ 0.05) in the FSH group between 4 h to 48 h compared to the values in the control one. In conclusion, a single administration of long-acting FSH enhanced testicular blood perfusion as measured by pulsed Doppler ultrasonography in rams during the non-breeding season. Concurrently, significant increases in the concentrations of E2 and NO were found.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"82 ","pages":"Article 106765"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33522517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}