慢性炎症会降低雄性绵羊弓状吻肽的表达。

IF 1.9 2区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
AN Renwick , BK Whitlock , CC Nestor , JA Daniel , L Strickland , AS Lear , M Adkins , C Griffin , A Esteller-Vico
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引用次数: 0

摘要

革兰氏阴性细菌产生的脂多糖(LPS)会诱发免疫反应,并通过抑制促性腺激素释放激素(GnRH)和随后的促黄体生成素(LH)的分泌来影响繁殖。虽然有证据表明急性炎症会抑制吻肽,但人们对慢性炎症对家畜这一关键生殖神经肽的影响知之甚少。因此,我们试图研究 LPS 抑制绵羊 LH 分泌的核心机制。20 只绵羊被随机分配到五个治疗组中的一个:对照组(CON;n=4)、单次急性静脉注射 LPS 剂量组(SAD;n=4)、每日急性静脉注射 LPS 剂量组(DAD;n=4)、每日增加静脉注射 LPS 剂量组(DID;n=4)和慢性皮下注射 LPS 剂量组(CSD;n=4)。在第 1 天和第 7 天,使用颈静脉穿刺每 12 分钟采集一次血样,持续 360 分钟。第7天采血后,对所有动物实施安乐术,用4%多聚甲醛灌注脑组织,取出下丘脑块并进行免疫组化处理。第1天,与CON(0.70±0.1脉冲/小时)相比,SAD(0.25±0.1脉冲/小时)、DAD(0.25±0.1脉冲/小时)、DID(0.35±0.1脉冲/小时)和CSD(0.40±0.1脉冲/小时)的LH脉冲频率明显较低(P=0.02)。在第 7 天,只有 DID 动物(0.35 ± 0.1 脉冲/小时)的 LH 脉冲频率显著低于对照组(0.85 ± 0.1 脉冲/小时)(p=0.049)。此外,与对照组(82.6 ± 13.6 个细胞/切片/只动物)相比,只有 DID 动物(33.3 ± 10.9 个细胞/切片/只动物)的吻肽免疫阳性细胞数量明显较少(p=0.001)。综上所述,我们认为每日增加剂量的 LPS 是年轻雄性绵羊吻肽神经元的强力抑制剂,是研究慢性炎症对家畜生殖轴影响的生理学相关模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chronic inflammation decreases arcuate kisspeptin expression in male sheep

Chronic inflammation decreases arcuate kisspeptin expression in male sheep

Lipopolysaccharide (LPS) from Gram-negative bacteria induces an immune response and impairs reproduction through suppression of gonadotropin releasing hormone (GnRH), subsequently luteinizing hormone (LH) secretion. While there is evidence that acute inflammation inhibits kisspeptin, little is known about the impact of chronic inflammation on this key reproductive neuropeptide in livestock species. Thus, we sought to examine a central mechanism whereby LPS suppresses LH secretion in sheep. Twenty wethers were randomly assigned to one of five treatment groups: control (CON; n=4), single acute IV LPS dose (SAD; n=4), daily acute IV LPS dose (DAD; n=4), daily increasing IV LPS dose (DID; n=4), and chronic subcutaneous LPS dose (CSD; n=4). On Days 1 and 7, blood samples were collected every 12 minutes for 360 minutes using jugular venipuncture. Following blood collection on Day 7, all animals were euthanized, brain tissue was perfused with 4% paraformaldehyde, and hypothalamic blocks were removed and processed for immunohistochemistry. On Day 1, LH pulse frequency was significantly lower (p=0.02) in SAD (0.25 ± 0.1 pulses/hour), DAD (0.25 ± 0.1 pulses/hour), DID (0.35 ± 0.1 pulses/hour), and CSD (0.40 ± 0.1 pulses/hour) compared to CON (0.70 ±0.1 pulses/hour). On Day 7, only DID animals (0.35 ± 0.1 pulses/hour) had significantly lower (p=0.049) LH pulse frequency compared to controls (0.85 ± 0.1 pulse/hour). Furthermore, only DID animals (33.3 ± 10.9 cells/section/animal) had significantly fewer (p=0.001) kisspeptin-immunopositive cells compared to controls (82.6 ± 13.6 cells/section/animal). Taken together, we suggest that daily increasing doses of LPS is a powerful inhibitor of kisspeptin neurons in young male sheep and a physiologically relevant model to examine the impact of chronic inflammation on the reproductive axis in livestock.

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来源期刊
Domestic animal endocrinology
Domestic animal endocrinology 农林科学-奶制品与动物科学
CiteScore
5.50
自引率
4.80%
发文量
58
审稿时长
31 days
期刊介绍: Domestic Animal Endocrinology publishes scientific papers dealing with the study of the endocrine physiology of domestic animal species. Those manuscripts utilizing other species as models for clinical or production problems associated with domestic animals are also welcome. Topics covered include: Classical and reproductive endocrinology- Clinical and applied endocrinology- Regulation of hormone secretion- Hormone action- Molecular biology- Cytokines- Growth factors
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