Evaluation of circulating miRNAs in mares approaching parturition

IF 1.9 2区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Mio Kikuchi , Harutaka Murase , Kenichi Urata , Taichiro Ishige , Shun-ichi Nagata , Teruaki Tozaki , Hironaga Kakoi , Toshina Ishiguro-Oonuma , Keiichiro Kizaki
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引用次数: 0

Abstract

Circulating microRNAs (miRNAs) are stable in body fluids and can serve as biomarkers for various diseases and physiological states. Although pregnancy˗related miRNAs have been identified in various mammals, studies on parturition˗related circulating miRNAs in mares are limited. Therefore, this study aimed to identify parturition˗related miRNAs and examine their potential applications in the prediction of parturition date. miRNAs were extracted from the plasma of Thoroughbred mares 30 days (295–326 days pregnant) and 5 (323–352 days pregnant) – 0 (328–357 days pregnant) days before parturition, followed by small RNA sequencing (small RNA˗seq) and reverse transcription quantitative PCR (RT˗qPCR). Additionally, we measured plasma progestin concentrations in mares using an enzyme-linked immunosorbent assay. Small RNA˗seq data indicated that 18 miRNAs were affected by parturition proximity. Among the 18 miRNAs, two novel miRNAs and three known miRNAs (miR˗361˗3p, miR˗483, and miR˗99a) showed significant changes at 5–0 days before parturition compared with that at 30 days to parturition. Plasma progestin concentrations were higher at 5–3 days to parturition than at 30 days to parturition, and then decreased on the day of parturition. Conclusively, this study provides basic knowledge of parturition˗related circulating miRNAs in mares, and identifies miRNAs that could potentially be used as biomarkers to predict parturition in mares.

评估临近分娩母马的循环 miRNAs
循环微RNA(miRNA)在体液中很稳定,可作为各种疾病和生理状态的生物标志物。虽然已在多种哺乳动物体内发现了与妊娠˗相关的 miRNA,但对母马分娩˗相关的循环 miRNA 的研究还很有限。因此,本研究旨在鉴定与临产相关的 miRNA,并研究它们在预测临产日期方面的潜在应用。从纯血马母马分娩前 30 天(怀孕 295-326 天)和 5 天(怀孕 323-352 天)- 0 天(怀孕 328-357 天)的血浆中提取 miRNA,然后进行小 RNA 测序(small RNA˗seq)和反转录定量 PCR(RT˗qPCR)。此外,我们还使用酶联免疫吸附试验测定了母马血浆中的孕激素浓度。Small RNA˗seq数据表明,18个miRNA受临产影响。在这18个miRNA中,2个新的miRNA和3个已知的miRNA(miR˗361˗3p、miR˗483和miR˗99a)在临产前5-0天与临产前30天相比有显著变化。血浆孕激素浓度在临产前 5-3 天高于临产前 30 天,并在临产当天下降。总之,这项研究提供了母马分娩˗相关循环 miRNA 的基本知识,并确定了可能用作预测母马分娩的生物标记的 miRNA。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Domestic animal endocrinology
Domestic animal endocrinology 农林科学-奶制品与动物科学
CiteScore
5.50
自引率
4.80%
发文量
58
审稿时长
31 days
期刊介绍: Domestic Animal Endocrinology publishes scientific papers dealing with the study of the endocrine physiology of domestic animal species. Those manuscripts utilizing other species as models for clinical or production problems associated with domestic animals are also welcome. Topics covered include: Classical and reproductive endocrinology- Clinical and applied endocrinology- Regulation of hormone secretion- Hormone action- Molecular biology- Cytokines- Growth factors
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