槲皮素对高浓度 CuSO4 诱导的猪卵巢颗粒细胞自噬和凋亡的影响

IF 1.9 2区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
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引用次数: 0

摘要

铜是维持生理功能所必需的重要微量元素。然而,过量的铜会导致器官损伤。高浓度的 CuSO4 会损坏猪卵巢颗粒细胞,从而降低母猪的繁殖能力。槲皮素在减轻重金属的有害影响方面具有显著功效。因此,本研究旨在探讨高浓度 CuSO4 对猪卵巢颗粒细胞自噬和凋亡的影响,以及槲皮素是否能抵消这些毒性作用。经200和400 µM CuSO4处理后,细胞形态以及自噬相关基因(LC3-Ⅰ、ATG5、ATG7、ATG12、Beclin1、mTOR、LC3-Ⅱ和P62)的mRNA表达水平均发生了显著变化。用 200 µM CuSO4 处理可增加 P62 蛋白的表达(P<0.05),促进 LC3-Ⅰ 向 LC3-Ⅱ 转化(P<0.05),降低 PINK1 蛋白的表达和 ATP 含量(P<0.05)。此外,Caspase3 蛋白表达增加,TUNEL 染色表明凋亡细胞数量增加。然而,与 10 µM 槲皮素联合处理可明显降低 P62 的表达和 LC3-Ⅰ 向 LC3-Ⅱ 的转化。此外,流式细胞分析表明,添加 10 µM 槲皮素可明显减少高浓度 CuSO4 诱导的细胞凋亡。 总之,研究结果表明,高浓度 CuSO4 可引发线粒体和自噬功能障碍,激活线粒体凋亡途径,并产生细胞毒性作用。槲皮素能缓解自噬功能障碍,增强自噬过程,减轻细胞凋亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of quercetin on autophagy and apoptosis induced by a high concentration of CuSO4 in porcine ovarian granulosa cells

Copper is a vital micronutrient necessary for the maintenance of physiological functions. However, excessive amounts can lead to organ damage. Porcine ovarian granulosa cells are damaged by a high concentration of CuSO4, which can reduce the reproductive capacity of sows. Quercetin has shown remarkable efficacy in mitigating the harmful effects of heavy metals. Therefore, the aim of this study was to investigate the effects of a high concentration of CuSO4 on autophagy and apoptosis in porcine ovarian granulosa cells and to explore whether quercetin can counteract these toxic effect. Cell morphology, and the mRNA expression levels of autophagy-related genes (LC3-Ⅰ, ATG5, ATG7, ATG12, Beclin1, mTOR, LC3-Ⅱ and P62) were significantly changed upon treatment with 200 and 400 µM CuSO4. Treatment with 200 µM CuSO4 increased expression of P62 protein (P<0.05), promoted LC3-Ⅰ to LC3-Ⅱ conversion (P<0.05), and reduced PINK1 protein expression and the ATP content (P<0.05). In addition, expression of Caspase3 protein was increased and TUNEL staining indicated that the number of apoptotic cells was increased. However, co-treatment with 10 µM quercetin significantly decreased expression of P62 and conversion of LC3-Ⅰ to LC3-Ⅱ. Furthermore, flow cytometric analysis revealed that addition of 10 µM quercetin significantly reduced apoptosis induced by a high concentration of CuSO4. In summary, the results indicate that a high concentration of CuSO4 can trigger mitochondrial and autophagy dysfunction, activate mitochondrial apoptosis pathway, and exert cytotoxic effects. Quercetin can mitigate autophagy dysfunction, enhance autophagic processes, and alleviate apoptosis.

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来源期刊
Domestic animal endocrinology
Domestic animal endocrinology 农林科学-奶制品与动物科学
CiteScore
5.50
自引率
4.80%
发文量
58
审稿时长
31 days
期刊介绍: Domestic Animal Endocrinology publishes scientific papers dealing with the study of the endocrine physiology of domestic animal species. Those manuscripts utilizing other species as models for clinical or production problems associated with domestic animals are also welcome. Topics covered include: Classical and reproductive endocrinology- Clinical and applied endocrinology- Regulation of hormone secretion- Hormone action- Molecular biology- Cytokines- Growth factors
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