Clinical and Experimental Dental Research最新文献

{"title":"RANKL, OPG, and CTS-K Release in Bone Response to Immediate Nonfunctional Loading of a Single Implant in Mandibular Molar Sites During Osseointegration Establishment","authors":"Xiaowen Hu,&nbsp;Yijie Fan,&nbsp;Xuexia Chen","doi":"10.1002/cre2.70193","DOIUrl":"https://doi.org/10.1002/cre2.70193","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To verify that osseointegration maturation under immediate restoration is correlative with active release of cytokines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The participants needing the restoration of a single missing mandibular molar were randomized into immediate restoration (IR) and conventional restoration group (CR). All eligible patients were recalled for collecting peri-implant crevicular fluid (PICF) samples according to the scheduled follow-up time point during osseointegration and functional loading. Detection of receptor activator of nuclear factor-KB ligand, osteoprotegerin, and cathepsin K in PICF was conducted to statistically analyze their difference between IR and CR groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>During the osseointegration period, the overall level of these cytokines in the IR group was statistically higher than that of the corresponding cytokine in the CR group. During functional loading, the overall level of the each cytokine in CR was statistically different from that of the corresponding cytokine during osseointegration, but the overall level of each cytokine was not statistically different between the two groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The rapid osseointegration maturation under immediate restoration is probably correlative with active release of cytokines related with bone metabolism.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144814785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Immediate Dentin Sealing With Various Universal Adhesives on Shear Bond Strength of Dual-Cure Resin Cement","authors":"Malin Janson,&nbsp;Li Sun,&nbsp;Anja Liebermann,&nbsp;Christoph Matthias Schoppmeier","doi":"10.1002/cre2.70186","DOIUrl":"https://doi.org/10.1002/cre2.70186","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study evaluated the effect of universal adhesives (UAs) applied as Immediate Dentin Sealing (IDS) on the shear bond strength (SBS) of dual-cure resin cement, both immediately and after thermocycling aging.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A total of 180 bovine incisors were prepared and randomly assigned to six groups (<i>n</i> = 15): NOC (control), Clearfil Universal Bond Quick (CUBQ), G-Premio Bond (GPB), Peak Universal Bond (PUB), Adhese Universal (AU), and Scotchbond Universal Plus (SBUP). Specimens were cemented with dual-cure resin cement (Panavia V5) and subjected to SBS testing at 24 h and after thermocycling (10,000 cycles, 5°C–55°C). SBS was measured using a universal testing machine, and failure modes were assessed microscopically. Statistical analysis was conducted using a Generalized Linear Model with Bonferroni correction (<i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant differences were found among UAs (<i>p</i> &lt; 0.001) and after aging (<i>p</i> &lt; 0.001). SBUP and GPB had the highest SBS before and after thermocycling, while NOC had the lowest. Thermocycling reduced SBS in all groups, with CUBQ and AU showing the largest declines. Failure mode analysis showed predominantly adhesive failures in NOC, while IDS groups had more cohesive and mixed failures.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>IDS technique with universal adhesives significantly enhances bond strength to dentin compared to conventional cementation. SBUP and GPB showed superior bonding performance, likely due to their monomer formulations containing 10-MDP and acetone, which are known to improve chemical adhesion to dentin and promote effective resin infiltration. Thermocycling led to a reduction in SBS across all groups, highlighting the impact of aging on adhesive durability, underscoring the importance of selecting UAs with lasting adhesive strength for long-term bonding.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70186","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Motivation for Smoking Cessation in Patients With Oral Squamous Cell Carcinoma—A One-Time Survey","authors":"Lennart Johannes Gruber,&nbsp;Matthias Maximilian Bühler,&nbsp;Antonie Spillner,&nbsp;Stefan Andreas,&nbsp;Philipp Kauffmann,&nbsp;Henning Schliephake,&nbsp;Susanne Wolfer","doi":"10.1002/cre2.70154","DOIUrl":"https://doi.org/10.1002/cre2.70154","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Tobacco smoking is one major risk factor in the development of oral squamous cell carcinoma (OSCC). Continuation of smoking after diagnosis and treatment is associated with an increase in recurrence rate and incidence of second tumors, with a shorter lon g-term survival and poorer response to therapy. In the current German guideline for the treatment of OSCC, there is no clear recommendation to participate in structured smoking cessation programs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 202 patients with histologically confirmed OSCC completed a one-time assessment of their smoking behavior using three standardized questionnaires during regular tumor follow-up. In addition to sociodemographic data, patients were asked retrospectively about their smoking habits and motivation to quit smoking before and after diagnosis and treatment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A serious smoking cessation attempt before diagnosis of OSCC were stated in 54.8% of the participants. This number increased up to 82.2% after OSCC diagnosis. However, only 48.5% managed to quit smoking after diagnosis. Professional support was with only 21.92% (<i>n</i> = 16) rarely used. Motivation to quit was significantly lower before (2.75 ± 2.41) than after OSCC diagnosis (7.27 ± 2.41) (<i>p</i> = 0.001) and significantly higher among the participants who finally managed to quit (9.38 ± 1.68) than among those who continued smoking (4.79 ± 3.43) (<i>p</i> = 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The diagnosis of OSCC appears to be an important teachable moment for smoking cessation. To maximize this effect, an early and standardized implementation of systematic smoking cessation programs into the therapeutic concept of patients with OSCC is strongly recommended.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rare Tumors of Oral Cavity: A Case Report and Literature Review on Secretory Carcinoma of Minor Salivary Glands","authors":"Emilio Salerno,&nbsp;Silvio Abati,&nbsp;Gianluigi Arrigoni,&nbsp;Daniela Finocchiaro,&nbsp;Giorgio Gastaldi,&nbsp;Alessandra Lissoni,&nbsp;Andrea Galli,&nbsp;Matteo Trimarchi","doi":"10.1002/cre2.70200","DOIUrl":"https://doi.org/10.1002/cre2.70200","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The primary objective of this study is to report and analyze two rare cases of secretory carcinoma (SC) of the minor salivary glands, focusing on their diagnostic and therapeutic work-up. The study aims to enhance scientific knowledge about SC, which is crucial for developing targeted therapies and ensuring precise diagnosis, especially differentiating it from acinic cell carcinoma (ACC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>The study involved a detailed examination of two patients diagnosed with SC of the minor salivary glands. Clinical examinations, histological investigations, and immunohistochemical analyses were conducted. The treatment approach included surgical excision of the lesions followed by regular follow-ups to monitor for recurrence. Immunohistochemical analysis was performed to identify the presence of markers such as GATA3, SOX-10, NTRK, mammaglobin, and others.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Two cases are shown: a case of a 76-year-old male with a lesion in the hard palate was initially misdiagnosed as leukoplakia. After surgical excision and histological examination, the lesion was identified as SC. The patient underwent follow-up examinations, including MRI and CT scans, which showed no recurrence, and another case of a 39-year-old female with a nodule in the superior left vestibule underwent surgery to remove the nodule. Histological and immunohistochemical analyses confirmed SC, showing a high proliferation index and presence of the ETV6–NTRK3 gene fusion. Follow-up imaging showed no signs of disease recurrence.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The study underscores the importance of precise diagnosis and differentiation of SC from ACC. Surgical excision followed by regular monitoring is effective in managing SC. Immunohistochemical and molecular analyses are crucial for accurate diagnosis. The findings contribute to the growing body of evidence on SC and highlight the potential for developing more targeted therapies. Further research is needed to establish clear guidelines for follow-up duration and treatment protocols.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70200","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cross-Sectional Study of the Quality of Online Information on Periodontal Surgery","authors":"William Weng Nian Mak,&nbsp;Sushil Kaur,&nbsp;Maurice J. Meade","doi":"10.1002/cre2.70195","DOIUrl":"https://doi.org/10.1002/cre2.70195","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To investigate the quality of online information provided by dental-related websites regarding periodontal surgery.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The term “Gum Surgery” was entered into three search engines (Google, Yahoo, and Bing). The content of websites satisfying selection criteria was assessed with five validated quality of information tools (DISCERN, The Patient Education Materials Assessment Tool [PEMAT], Journal of the American Medical Association [JAMA] benchmarks, and HONCode and @TRUST certification). The Simple Measure of Gobbledygook (SMOG) was used to evaluate the readability of content.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 55 websites satisfied selection criteria. The mean (SD) DISCERN score for all website categories was 2.89 (0.57). The quality of information related to the risks of each treatment scored poorly in most websites. The healthcare portals obtained the highest mean PEMAT score of 71.74%, a statistically significant outcome. Healthcare portal websites also recorded the highest mean (SD) JAMA score of 3.72 (0.75) out of 4. The mean (SD) SMOG score was 9.56 (1.07). Cohen's <i>κ</i> inter-rater reliability for DISCERN and PEMAT scores were 0.75 and 0.79, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The information available online about periodontal surgery was variable and difficult to read, falling short of established standards for accuracy, reliability, and credibility. Vital information was often omitted.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of 3D-Printed Polylactic Acid as a Bone Substitute: An Animal Study in a Rat Model","authors":"Velayudhan Ashok,&nbsp;Mohanraj Karthik Ganesh,&nbsp;Subhabrata Maiti,&nbsp;Deepak Nallaswamy,&nbsp;Artak Heboyan","doi":"10.1002/cre2.70201","DOIUrl":"https://doi.org/10.1002/cre2.70201","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Bone repair and regeneration are important processes for treating bone defects and injuries. However, traditional bone grafts like autografts and allografts have limitations, such as complications at the donor site and immune rejection. As a result, there is growing interest in using polylactic acid (PLA), a biodegradable and biocompatible material, as a synthetic bone substitute. This study aims to evaluate the effectiveness of 3D-printed PLA scaffolds as bone substitutes using a rat model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>PLA scaffolds with dimensions of 2 × 2 × 4 mm and 2 × 2 × 8 mm were fabricated using the CUBEX-TRIO 3D printer. Twelve male Wistar rats were divided into four groups based on defect size (4 and 8 mm) and observation period (4 weeks and 8 weeks). The surgical procedures involved creating discontinuity defects in the rats' zygoma and implanting PLA scaffolds that were stabilized with a bio-membrane. Bone regeneration was assessed through radiographic analysis and histological examination.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Radiographic analysis confirmed the formation of bone in the grafted regions. Histological analysis revealed connective tissue formation at the defect edges and scaffold surface at both 4 and 8 weeks. In the 4 mm defect group, the transformation of connective tissue into chondrocytes and endochondral ossification was observed at 8 weeks, indicating successful bone regeneration. However, in the 8 mm defect group, bone formation was not as evident, suggesting limitations in the osteoinductive potential of PLA scaffolds for larger defects.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The 3D-printed PLA scaffolds show promise as bone substitutes for small to moderate-sized defects due to their effective biocompatibility and osteoinductive potential. Further studies are needed to optimize their performance for larger defects, potentially enhancing their clinical application in bone repair and regeneration.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Low-Intensity Electrical Stimulation on External Apical Root Resorption and Periodontal Indices Following En-Masse Retraction of Upper Anterior Teeth in Young Adults: A Randomized Controlled Trial","authors":"Rashad I. Shaadouh,&nbsp;Mohammad Y. Hajeer,&nbsp;Mohammad Khursheed Alam,&nbsp;Samer T. Jaber","doi":"10.1002/cre2.70188","DOIUrl":"https://doi.org/10.1002/cre2.70188","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Objective&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;To evaluate external apical root resorption (EARR) and periodontal indices during en-masse retraction of maxillary anterior teeth stimulated with low-intensity electrical currents.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Trial Design&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;A two-arm randomized controlled trial.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Young adult patients who visited the Department of Orthodontics at Damascus University between November 2023 and March 2024 and met predefined inclusion criteria were randomly allocated into two groups using block randomization. The study included 34 patients, with 17 assigned to the electrically stimulated en-masse retraction (ESER) group and 17 to the conventional en-masse retraction (CER) group. The force for the en-masse retraction technique in both groups was 250 g per side. The force was applied through bilateral closed-coil nickel-titanium springs anchored to an orthodontic mini-screw on each side. In the ESER group, each upper anterior tooth was subjected to a continuous electrical stimulation of 15–20 µA for 5 h daily, utilizing an intraoral removable electrical stimulation device. EARR was assessed using digital panoramic radiographs. Four periodontal indices were also used to assess periodontal status. Blinding was confined to data analysis.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Thirty-four patients (26 females and 8 males) were analyzed with a mean age of 21.12 ± 2.41 years. At the end of the en-masse retraction phase, no significant difference in EARR was observed between the two groups (&lt;i&gt;p&lt;/i&gt; &gt; 0.05). The observed root resorption in the ESER and CER ranged between 0.27 and 0.64 and 0.32 and 0.71 mm, respectively. Also, insignificant differences were found in all periodontal indices studied at all measurement points between the two groups (&lt;i&gt;p&lt;/i&gt; &gt; 0.05).&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusions&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Low-intensity electrical stimulation had no significant effect on root resorption during the en-masse retraction of the six upper anterior teeth. Both groups exhibited comparable slight root resorption without any significant difference between them. Additionally, low-intensity electrical stimulation did not affect the periodontal status during en-masse retraction.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Trail Registration&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Clinical Trials database (NCT06873490).&lt;/p&gt;\u0000 ","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Silver Diamine Fluoride Application Methods on Dentin Microhardness and Durability Under pH Cycling: An In Vitro Study","authors":"Farideh Darabi,&nbsp;Parmida Farzam,&nbsp;Mehrsima Ghavami-Lahiji","doi":"10.1002/cre2.70190","DOIUrl":"https://doi.org/10.1002/cre2.70190","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study investigated the effects of various application methods of silver diamine fluoride (SDF) on dentin microhardness and its durability under a 14-day acid challenge. The primary goal was to identify an optimal conservative SDF application protocol for clinical dentistry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Mid-coronal dentin disc samples from human premolars were prepared through two horizontal cuts and polished. Samples were randomly divided into three groups of ten: (1) SDF-NR (SDF applied for 3 min, excess removed with cotton); (2) SDF-R-R (SDF applied, rinsed, and polished after 24 h); (3) SDF-R (SDF applied for 3 min, then rinsed). Vickers microhardness was measured at three stages: initial, post-demineralization, and post-SDF application. In the second phase, a control group (no SDF) and a test group (SDF-R) underwent a 14-day pH-cycling regimen, with final hardness changes evaluated at a significance level of 0.05.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant microhardness increases were observed in all groups following SDF application compared to demineralized conditions (<i>p</i> = 0.001). Although the decrease in microhardness after acid challenge in the SDF group was less than in the control group, this difference was not statistically significant (<i>p</i> = 0.423).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Different SDF application methods significantly enhance dentin microhardness post-demineralization. Nevertheless, the acid challenge revealed minimal differences between the control and test group, indicating that reapplication of SDF may be necessary for sustained effectiveness.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Antimicrobial Properties of Different Dental Cleaning Tablets Against S. aureus Grown on Two Denture Base Materials","authors":"Sindi Vishaj,&nbsp;Alissa Villhauer,&nbsp;Mandi Morris,&nbsp;Donald M. Belles,&nbsp;Daher Antonio Queiroz,&nbsp;Maria D. Gonzalez,&nbsp;Ransome van der Hoeven","doi":"10.1002/cre2.70189","DOIUrl":"https://doi.org/10.1002/cre2.70189","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to evaluate the antimicrobial efficacy of three commercially available denture cleaning tablets Efferdent, Polident, and Val-clean against <i>Staphylococcus aureus</i> biofilms formed on two denture base materials: milled polymethyl methacrylate (PMMA) and 3D printed denture resin.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Eighty samples were fabricated, with 40 specimens per denture base material: CAD/CAM milled PMMA (Lucitone Digital Fit LT; Dentsply Sirona, Charlotte, NC, USA) and 3D printed resin (Denture Base Resin; Formlabs Inc., Somerville, MA, USA). Each group was divided into four subgroups (<i>n</i> = 10), Efferdent, Polident, Val-clean, and deionized water (control). Surface roughness was measured using a digital profilometer (Digiprofilo I; Digiwork Instruments, Concord, ON, Canada). Samples were exposed to <i>S. aureus</i> in tryptic soy broth (TSB) and subsequently treated with the cleaning solutions. Colony-forming units (CFUs) were quantified using serial dilution plating. To distinguish bactericidal from bacteriostatic effects, normalized <i>S. aureus</i> cultures were incubated with the cleaning agents for 16 h, followed by optical density measurements, LIVE/DEAD staining (Invitrogen, Carlsbad, California), and TSB agar plating. Statistical analysis was performed using one-way ANOVA and Dunnett's multiple comparisons test, with significance set at <i>p</i> &lt; 0.05.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A significant difference in surface roughness was observed between the 3D printed and milled samples before treatment (<i>p</i> &lt; 0.0001). Significantly fewer CFUs were observed in all cleaning solution groups compared to the control (<i>p</i> &lt; 0.05). All denture cleaning solutions demonstrated bactericidal activity against <i>S. aureus</i>, as indicated by the absence of CFU growth on agar plates and the lack of viable cells in LIVE/DEAD staining posttreatment. Both denture materials demonstrated comparable microbial adhesion and response to the cleaning agents.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Efferdent, Polident, and Val-clean denture tablets are effective bactericidal agents against <i>S. aureus</i> in vitro, regardless of the denture base material used. These findings support their use in daily denture hygiene routines, particularly in reducing bacterial colonization on removable prostheses.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Clinical Efficacy of the Application for Articulation Therapy-Thai (AAT-T) for Thai Children With Cleft Lip and Palate","authors":"Benjamas Prathanee,&nbsp;Sumita Duangprasert,&nbsp;Sasalaksamon Chanachai,&nbsp;Patorn Piromchai","doi":"10.1002/cre2.70194","DOIUrl":"https://doi.org/10.1002/cre2.70194","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Mobile applications are becoming essential for speech therapy, especially in areas with limited access to professional speech therapists. This technological intervention is especially pertinent during pandemics, which further restrict access to traditional therapeutic modalities. The objective of this study was to develop and evaluate a mobile application for articulation therapy, specifically designed for pediatric patients with articulation disorders associated with cleft palate.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The articulation exercises incorporated in the application encompassed 27 Thai initial and final consonant sounds, presented through video demonstrations and tabular reading materials. These materials included nonsense syllables, words, phrases, and sentences. The content validity was assessed using the Item-Objective Congruence (IOC) index. Face validity and functional satisfaction were evaluated by a panel comprising four speech-language pathologists (SLPs), five children with cleft palate with or without cleft lip (CP ± L) and their respective caregivers, and an expert in application design. The clinical efficacy of the application for speech correction was subsequently assessed in a cohort of 19 children diagnosed with CP ± L.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The Application for Articulation Therapy-Thai (AAT-T) was finalized after four iterative revisions. The IOC index for the application ranged from 0.80 to 1.00, indicating strong content validity. Functional satisfaction scores ranged from 76% to 100%, suggesting high user acceptability. Nineteen children participated in the clinical efficacy assessment; however, one child was withdrawn due to the inability to complete 6-month follow-up. The results demonstrated that AAT-T significantly reduced articulation errors in connected speech (mean difference = 2.33, SD = 2.40, 95% CI = 1.14–3.53, <i>p</i> &lt; 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The AAT-T application emerged as an accessible, engaging, and motivational tool for articulation practice. Its efficacy in speech correction was demonstrated.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70194","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}