Clinical and Experimental Dental Research最新文献

{"title":"Marginal Gap of Pre-Cemented Endocrowns: A Systematic Review of Measurement Methods and the Influence of Fabrication Method and Crown Material","authors":"James Dudley, Taseef Hasan Farook","doi":"10.1002/cre2.70152","DOIUrl":"https://doi.org/10.1002/cre2.70152","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The aim of this systematic review was to analyze the existing literature on the methods used for in vitro marginal gap measurement of pre-cemented endocrowns and determine whether the fabrication method and material used influenced the marginal gap.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Systematic screening was conducted until January 2025 using EBSCO Host, Scopus, PubMed, and Web of Science databases. This systematic review followed PRISMA guidelines, including in vitro studies on marginal gaps in single-unit pre-cemented endodontic crowns while excluding in vivo and virtual assessments, and studies analyzing preformed and implant-supported crown assessments. The quality of the selected studies was evaluated using the Joanna Briggs Critical Appraisal Checklist. Welch's <i>t</i>-test and ANOVA were used to analyze differences in marginal gaps across the included variables.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Twenty-eight studies were included for analysis. The mean marginal gap for endocrowns across all included studies was 82.75 ± 33.10 µm. Endocrowns fabricated on maxillary teeth demonstrated a mean marginal gap of 77.01 ± 25.46 µm compared with 85.05 ± 36.04 µm for mandibular teeth (<i>t</i> = −0.66, <i>p</i> = 0.513). Endocrowns fabricated using computer-aided design/computer-aided manufacturing (CAD/CAM) (86.65 ± 38.14 µm) had significantly smaller marginal gaps compared with those produced using conventional impressions (109.37 ± 30.05 µm) (<i>t</i> = 2.746, <i>p</i> = 0.038). Seven measurement methods were reported, with significant differences observed in marginal gap values across the different methods (<i>F</i> = 4.61, <i>p</i> = 0.013). Impression replica and stereomicroscopy were the most used marginal gap methods, used collectively in 19 (68%) of the included 28 studies. Impression replica (95.49 ± 31.57 µm) and stereomicroscopy (63.69 ± 26.81 µm) methods produced significantly different marginal gap measurements (<i>p</i> = 0.003). There were no significant differences in marginal gap between endocrown materials (<i>p</i> = 0.122), with marginal gaps ranging from 60.93 ± 26.66 µm in the less explored polymer-infiltrated ceramic to 95.21 ± 34.07 µm in the more frequently studied zirconia. Lithium disilicate remained the most heavily researched material with a pooled marginal gap of 84.04 ± 32.91 µm across all included studies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The choice of measurement technique significantly influenced the reported marginal gap value","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic Reviews Comparing Direct and Indirect Restorations: An Umbrella Review That Examines Restoration Type and Confidence in Results","authors":"Mona Kimmel,&nbsp;Clovis Mariano Faggion Jr.","doi":"10.1002/cre2.70149","DOIUrl":"https://doi.org/10.1002/cre2.70149","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>There are two technologies for restoring individual structurally defective teeth. A direct restoration is applied chairside in one appointment, while an indirect restoration needs to be lab-produced before application. This umbrella review of previous English systematic reviews was conducted to determine if there was any difference between the two restoration types in adults regarding failure and the review methodological quality.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>On November 7, 2023, three databases (PubMed, Web of Science, and Cochrane) were searched for systematic reviews comparing direct and indirect restorations. Two researchers independently selected the studies and extracted the information from the full texts of the articles. The methodological quality of the reviews was assessed with the measurement tool to assess systematic reviews (AMSTAR-2) checklist.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Initially, 436 articles were identified; after screening, a total of 20 systematic reviews were included. Overall, the reviews indicated that both restorations produced similar results. There was a preference for direct restoration of small defects and indirect restoration for teeth with fewer than two remaining walls. Direct restoration was more cost and production-efficient, but indirect restoration offered better long-term results for larger defects. The overall confidence in the systematic review results ranged from critically low to high.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Either restoration is a valid option for restoring damaged teeth. The success of the restoration depends on the patient, operator, remaining tooth structure, and restoration material. Because of the high heterogeneity of patients, teeth, and material factors, there is no recommendation on the restoration type.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gingival Stem Cell-Conditioned Media and Low-Level Laser Therapy Enhance Periodontal Ligament Stem Cells Function by Upregulating Wnt and TGF-β Pathway Components: An In Vitro Study","authors":"Mohammed Y. Aljabri,&nbsp;Yaser A. Alhazmi,&nbsp;Salah A. Elsayyad,&nbsp;Shehabeldin M. Saber,&nbsp;Mohamed Shamel","doi":"10.1002/cre2.70151","DOIUrl":"https://doi.org/10.1002/cre2.70151","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study investigated the synergistic effects of gingival stem cell conditioned media (GSC-CM) and low-level laser therapy (LLLT) on stimulating PDLSCs, explicitly focusing on the molecular basis of enhancing the Wnt and TGF-β signaling pathways.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>PDLSCs were isolated and GSC-CM were prepared and characterized. Five groups were utilized: control, osteogenic induction medium (positive control), GSC-CM, LLLT (980 nm, 1.5 J/cm²), and a combination of GSC-CM with LLLT. Cell viability was assessed using the MTT assay on Days 1, 2, and 3. Osteogenic differentiation of PDLSCs was assessed using mineralization assays employing Alizarin Red staining, and molecular analyses of osteogenic markers (<i>RUNX2, ALP, OCN</i>) and signaling-related genes (<i>CTNNβ1, TGFβ1</i>) conducted via RT‒PCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The findings revealed that the dual application of GSC-CM and LLLT significantly enhanced cell viability and osteogenic differentiation of PDLSCc compared to the effects of the individual treatment modalities. Moreover, the study revealed elevated expression levels of osteogenic markers across all the experimental groups, specially the combination group, which showed the highest levels. Significantly, the combination treatment group displayed superior outcomes in this regard. The results also suggest potential activation of Wnt/β-catenin and TGF-β signaling components.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study highlights the significant effects of using GSC-CM and LLLT to improve PDLSC survival and osteogenic differentiation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computer-Assisted Implant Surgery: Patients' Experience and Perspectives","authors":"Xin Hui Yeo,&nbsp;Lin Jing Uei,&nbsp;Man Yi,&nbsp;Kajorn Kungsadalpipob,&nbsp;Keskanya Subbalehka,&nbsp;Bilal Al-Nawas,&nbsp;Nikos Mattheos","doi":"10.1002/cre2.70143","DOIUrl":"https://doi.org/10.1002/cre2.70143","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Although computer-assisted implant surgery (CAIS) has increased significantly the precision of dental implant placement, documentation of the impact of such technologies in the patient-reported experience and outcomes remains, however, limited. The aim of this white paper was to assess the impact of CAIS on key aspects of the patient experience, such as its potential benefits on (1) patients' understanding and engagement with implant surgery, (2) patient's confidence with treatment outcomes, (3) patients' preferences, (4) intra- and (5) Postoperative experience and (6) long-term patient-reported outcomes and oral health-related quality of life.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A review of the literature compiled existing evidence from clinical studies up to November 2024, which was later discussed and synthesized with expert opinions and the best currently documented experience and practice.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>No evidence was found that CAIS improves patient engagement or confidence with treatment outcomes, while comparative studies showed no difference in the intra- and postoperative experience when CAIS is used. Impact of the cost of CAIS procedures on patients perceptions and preferences has also not been explored, with the majority of studies significantly subsidizing patient costs, in particular randomized trials. At the same time, studies that compare interventions cannot assess the overall benefits of a complex workflow such as immediacy or minimally invasive approaches, to which CAIS is an essential part.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Research on patient outcomes with CAIS might not fully reflect the potential of these technologies when limited to the level of the surgical intervention. Major anticipated benefits of CAIS for the patient such as the potential to reduce complexity and facilitate faster, safer and more predictable execution of digitally designed treatments, could be better approached in the future by studies aimed at assessing patient-reported outcomes from entire treatment workflows.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Intelligence and Hand Hygiene Accuracy: A New Era in Infection Control for Dental Practices","authors":"Salwa A. Aldahlawi,&nbsp;Amr H. Almoallim,&nbsp;Ibtesam K. Afifi","doi":"10.1002/cre2.70150","DOIUrl":"https://doi.org/10.1002/cre2.70150","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The study aimed to assess the efficacy of an artificial intelligence (AI) model in evaluating hand hygiene (HH) performance compared to infection control auditors in dental clinics.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Method</h3>\u0000 \u0000 <p>The AI model utilized a pretrained convolutional neural network (CNN) and was fine-tuned on a custom data set of videos showing dental students performing alcohol-based hand rub (ABHR) procedures. A total of 66 videos were recorded, with 33 used for training and 11 for validating the model. The remaining 22 videos were designated for testing and the AI- infection control auditors comparison experiment. Two infection control auditors assessed the HH performance videos using a standardized checklist. The model's performance was evaluated through precision, recall, and F1 score across various classes. The level of agreement between the auditors and the AI assessments was measured using Cohen's kappa, and the sensitivity and specificity of the AI were compared to those of the infection control auditors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The AI model has learned to differentiate between classes of hand movement, with an overall F1 score of 0.85. Results showed a 90.91% agreement rate between the AI model and infection control auditors in evaluating HH steps, with a sensitivity of 85.7% and specificity of 100% in identifying acceptable HH practices. Step 3 (back of fingers to opposing palm with fingers interlocked) was consistently identified as the most frequently missed step by both the AI model and the infection control auditors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The AI model assessment of HH performance closely matched auditors' evaluations, suggesting its reliability as a tool for evaluating and mentoring HH in dental clinics. Future research should explore the application of AI technology in different dental settings to further validate its feasibility and adaptability.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medication Purchases Are Associated With the Number of Dental Treatments","authors":"Freja Frankenhaeuser,&nbsp;Håkan Källmén,&nbsp;Jukka Meurman,&nbsp;Esa Korpi,&nbsp;Birgitta Söder","doi":"10.1002/cre2.70121","DOIUrl":"https://doi.org/10.1002/cre2.70121","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Little is known whether the number of systemic medications, indicating worsened general health, affects the number of dental appointments needed. The hypothesis is that patients purchasing more systemic medications would have an increased number of dental appointments and respective need for treatments than patients who do not purchase as many medications.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Our cohort consists of 1495 participants from the Stockholm area, Sweden, initially examined in 1985. Using national population and patient registers (2005–2017), the association between the number of medication purchases and dental appointments was analyzed. The Anatomical Therapeutic Chemical Classification System for Medicines (ATC) was used. Descriptive statistics, chi-square tests, and logistic regressions were used with several covariates like gender and socioeconomic status.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Purchases above the median of all medications showed a statistically significant association with more dental appointments and respective treatments. Most of the ATC system's main drug categories were significantly associated with more dental appointments, even when adjusting for covariates. Purchases of medications from 32 different ATC subgroups were significantly associated with the number of dental appointments above the median.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In the group of adult Swedes who were studied, it was found that increased purchasing of nearly all types of drugs was associated with an increased number of dental appointments between the study years 2005–2017.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Evaluation of Flexural Strength, Impact Strength, and Surface Microhardness of Vaccinium macrocarpon Reinforced Polymethyl Methacrylate Denture Base Resin","authors":"Anitha Kuttae Viswanathan,&nbsp;Rajkumar Krishnan","doi":"10.1002/cre2.70145","DOIUrl":"https://doi.org/10.1002/cre2.70145","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The antimicrobial efficacy of cranberry (CA) against oral infections was well evidenced. Influence of cranberry on the mechanical properties of heat-activated polymethyl methacrylate (HA-PMMA) denture base resin (DBR) is unexplored till date and is significant for a better understanding of the reinforcement. This study aimed to evaluate the flexural strength (FS), impact strength (IS), and surface microhardness (VHN) of heat-cure PMMA DBR reinforced with varying concentrations of <i>Vaccinium macrocarpon</i> (cranberry) extract.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A total of 150 samples were categorized into five groups (<i>n</i> = 10) by weight percentage of 0, 0.5, 1.0, 1.5, and 2.0 cranberry extract added into HA-PMMA polymer after the performance of antimicrobial efficacy testing of CA. Three-point bending test for FT, Izod impact testing for IS, and Vickers microhardness test were performed. Fractured sample surface was characterized by a high-resolution scanning electron microscope (HR-SEM). Raw data were statistically analyzed with one-way ANOVA and post hoc Bonferroni test.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A significant improvement in flexural strength of 76.88 ± 0.73 MPa, impact strength of 6.66 ± 0.24 kJ/m², and microhardness of 18.44 ± 0.27 kg/mm² was observed at 2 wt.% (<i>p</i> &lt; 0.0001). Fractured surface topography showed dispersion of cranberry particles as a thin fibrous band intermeshed within resin matrix.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Addition of up to 2 wt.% cranberry improved the FS, IS, and VHN on comparison to 0 wt.% control HA-PMMA.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Methylation of iNOS, eNOS, TP53 and LINE-1 in Gingival Tissues of Cigarette Smokers, Heat-Not-Burn Smokers and Never Smokers: An Exploratory Study","authors":"Giulio Rasperini,&nbsp;Michele Codari,&nbsp;Alessandra Moiana,&nbsp;Letizia Tarantini,&nbsp;Valentina Bollati,&nbsp;Gionata Bellucci,&nbsp;Dario Consonni,&nbsp;Lena Larsson,&nbsp;Farah Asa'ad","doi":"10.1002/cre2.70141","DOIUrl":"https://doi.org/10.1002/cre2.70141","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>In this exploratory study, the influence of traditional cigarette smoking and heat-not-burn device smoking (HnB) on DNA methylation of cancer-associated genes in smokers compared to never smokers was evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials &amp; Methods</h3>\u0000 \u0000 <p>Twenty-eight healthy individuals undergoing dental care were enrolled into three groups: (i) 10 traditional smokers, (ii) 8 HnB smokers, and (iii) 10 never smokers. Gingival biopsies were obtained, and DNA methylation analysis was performed by pyrosequencing for the cancer-associated genes: <i>iNOS</i>, <i>eNOS</i> and <i>TP53</i>. LINE-1 sequence was selected for genome-wide methylation readout.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>HnB smokers exhibited approximately a 13% higher <i>iNOS</i> methylation compared to both traditional smokers and never smokers (<i>p</i> = 0.004 in a crude analysis and <i>p</i> = 0.01 in a multivariable linear regression model adjusted for gender and age). However, HnB smoking did not influence the DNA methylation levels of <i>TP53, eNOS</i>, and LINE-1 in gingival tissues, as these were comparable to the levels observed in traditional smokers and never smokers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>HnB device smoking increased DNA methylation levels of <i>iNOS</i> gene, which might indicate a decreased iNOS expression in HnB smokers, compared to traditional smokers and never smokers. Implications of the observed <i>iNOS</i> methylation status in the development of oral cancer needs to be investigated in future studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond Accuracy: Clinical Outcomes of Computer Assisted Implant Surgery","authors":"Sofya Sadilina,&nbsp;Kay Vietor,&nbsp;Romain Doliveux,&nbsp;Adam Siu,&nbsp;Zhuofan Chen,&nbsp;Bilal Al-Nawas,&nbsp;Nikos Mattheos,&nbsp;Allesandro Pozzi","doi":"10.1002/cre2.70129","DOIUrl":"https://doi.org/10.1002/cre2.70129","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Computer Assisted Implant Surgery (CAIS) with different technologies and modalities is becoming increasingly utilized in clinical practice. The aim of this White Paper was to synthesize evidence, reported experience, and best practices with regard to clinically relevant outcomes of static, dynamic, and robotic CAIS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A review of the literature compiled existing evidence from clinical studies up to November 2024, which was later discussed and synthesized into clinically relevant questions with a panel of international experts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There is overwhelming evidence for the superiority of static, dynamic, and robotic CAIS with regard to the accuracy of implant placement and some limited evidence of superior esthetic outcomes. At the same time, outcomes related to implant primary stability, survival rates, intra- and postoperative complications, marginal bone loss, and peri-implant tissue health appear similar between guided and non-guided implant surgery, while efficiency is poorly defined and studied. The importance of accuracy in the execution of a comprehensive, prosthetically driven treatment plan is not reflected in most studies, which focus mainly on the assessment of procedures rather than entire treatment workflows. Such inherent limitations of available research might conceal some of the potential of guided CAIS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Guided CAIS can achieve at least as good clinical outcomes as non-guided implant surgery. Studies that can assess the benefits of CAIS as part of a treatment workflow, rather than isolated procedures, could improve our understanding of the potential of these technologies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Assessment of Salivary Pellicle Disruption and Biofilm Removal on Titanium: Exploring the Role of Surface Hydrophobicity in Chemical Disinfection","authors":"Wenji Cai,&nbsp;Azam Fayezi Sisi,&nbsp;Mohamed-Nur Abdallah,&nbsp;Ashwaq A. Al-Hashedi,&nbsp;Juan Daniel Gamonal Sánchez,&nbsp;Enrique Bravo,&nbsp;Hasna H. Kunhipurayil,&nbsp;Rubens Albuquerque,&nbsp;Zahi Badran,&nbsp;Mariano Sanz,&nbsp;Faleh Tamimi","doi":"10.1002/cre2.70082","DOIUrl":"https://doi.org/10.1002/cre2.70082","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Peri-implantitis is mostly caused by a pathological biofilm that forms through complex processes, initiated by the formation of the salivary pellicle on implant surfaces. Understanding the nature of these pellicles and biofilm and how to remove them is important for preventing peri-implant infections and improving the success of dental implants. This study explores the characteristics of the salivary pellicle on titanium surfaces and assesses the effectiveness of different decontamination agents in eliminating the salivary pellicle and related microbial contaminations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Titanium surfaces were contaminated with salivary pellicles and pathological biofilms. The nature of the salivary pellicle was characterized using X-ray photoelectron spectroscopy (XPS), surface proteomics, contact angle measurements, and fluorescence microscopy. We tested six commonly used decontamination chemicals (chlorhexidine, essential oil-based mouthwash, citric acid, phosphoric acid, saline, and phosphate buffer saline) as well as newly proposed treatments such as surfactants and solvents (acetone, acetic acid, and Tween 20) for their capability to eliminate salivary pellicles and pathogenic biofilms from titanium surfaces.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The hydrophobic nature of the salivary pellicle on titanium surfaces limits the efficacy of commonly used hydrophilic solutions in removing pellicles and bacteria. Organic solvents and surfactants, particularly acetic acid and Tween 20, demonstrated superior effectiveness in removing the pellicle and biofilm. Acetic acid was notably effective in restoring surface composition, reducing microbial levels, and removing multispecies biofilms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The use of surfactants and solvents could be a promising alternative for the treatment of biofilms on titanium surfaces. However, further studies are needed to explore their clinical applicability.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143926092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}