Clinical and Experimental Dental Research最新文献

{"title":"Effects of Stromal Cell-Derived Factor 1 on Osteoblast Activity During Orthodontic Tooth Movement.","authors":"Yoshiyuki Hamada, Yuji Ishida, Albert Chun-Shuo Huang, Kenta Hamahara, Duangtawan Rintanalert, Kai Li, Kasumi Hatano-Sato, Jun Hosomichi, Risa Usumi-Fujita, Takashi Ono","doi":"10.1002/cre2.70425","DOIUrl":"10.1002/cre2.70425","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to clarify the relationship between stromal cell‑derived factor 1 (SDF‑1) and osteoblast-related responses in periodontal ligament (PDL) during orthodontic tooth movement (OTM) after tooth extraction, based on the hypothesis that local inhibition of SDF‑1/C‑X‑C chemokine receptor type 4 (CXCR4) signaling with the CXCR4 antagonist AMD3100 would attenuate osteoblast differentiation and alveolar bone remodeling during OTM.</p><p><strong>Methods: </strong>Thirty-six 6-week-old Wistar/Sprague-Dawley male rats were randomly divided into control and experimental groups. After the extraction of the maxillary left first molar, the maxillary left second molar (M2) was moved mesially with a 10-gf force following the local injection of phosphate-buffered saline or AMD3100 into the buccal and palatal gingiva adjacent to the M2 in the control and experimental group, respectively. Micro-computed tomography was performed to assess tooth movement and alveolar bone changes. Histological and immunohistochemical analyses were performed to evaluate periodontal tissue morphology and the percentages of SDF-1-, CXCR4-, RUNX2-, and BGLAP-positive cells on the tension side of the PDL. Quantitative reverse transcription PCR was used to assess expression levels of SDF-1, CXCR4, RUNX2, and BGLAP.</p><p><strong>Results: </strong>OTM distance and mesial tilt angle were significantly reduced in the experimental group. RT-PCR showed that SDF-1, CXCR4, and RUNX2 expression levels were significantly downregulated from Day 1 through Day 7, whereas BGLAP expression was significantly decreased only on Day 1. Immunohistochemical analysis of the tension side of the PDL demonstrated significantly reduced SDF-1- and CXCR4-positive cells on Day 3 and RUNX2-positive cells on Day 7 in the experimental group. In contrast, the BGLAP-positive cells did not differ significantly at any time point.</p><p><strong>Conclusion: </strong>Local inhibition of SDF-1/CXCR4 signaling reduced OTM and altered early alveolar bone remodeling after tooth extraction. The combined gene expression and immunohistochemical findings suggest that the SDF-1/CXCR4 axis may contribute to osteoblast differentiation, particularly through RUNX2-related responses during the early phase of OTM.</p><p><strong>Trial registration: </strong>Not applicable.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70425"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13472516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148759304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Ozonated Gels and Oils As Adjunctive or Monotherapy in Periodontitis: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.","authors":"Mahdi Bahrami, Mahsa Etemadi, Reyhaneh Abrishami, Farshad Alipour, Shiva Hadizadeh, Faezeh Jamshidian, Nooshin Abedinzadeh, Abolfazl Akbari, Edris Pordel","doi":"10.1002/cre2.70439","DOIUrl":"10.1002/cre2.70439","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the available clinical evidence on the use of ozonated gels and oils in the management of periodontitis.</p><p><strong>Material and methods: </strong>Embase, SCOPUS, Web of Science, PubMed, and Google Scholar were searched up to May 13, 2025. Original peer-reviewed randomized controlled trials (RCTs) in English that investigated patients with periodontitis were included. Study characteristics, methods of ozone application, conclusions, and reported complications were extracted. Pooled mean differences of clinical indices were compared between the ozone therapy groups (adjunctive or monotherapy) and control groups (placebo or CHX). Primary outcomes included clinical indices such as probing pocket depth (PPD), plaque index (PI), and gingival index (GI), whereas secondary outcomes comprised clinical attachment level (CAL), clinical attachment loss (CAtL), bleeding on probing (BoP), and sulcus bleeding index (SBI).</p><p><strong>Results: </strong>Of the 962 records, 19 clinical studies were included in the qualitative synthesis and 18 RCTs in the quantitative synthesis. For periodontitis, ozonated compounds significantly reduced PPD when used as an adjunct to subgingival instrumentation (MD = -0.780; p < 0.0001) and reduced clinical attachment loss (CAL) (MD = -0.457; p = 0.001) compared with subgingival instrumentation alone. No statistically significant differences were observed in PI or GI outcomes with ozonated compounds as an adjunct to subgingival instrumentation compared with subgingival instrumentation alone, although sensitivity analyses indicated some instability in the results. Follow-up periods ranged from 4 to 12 weeks across outcomes (8-12 weeks for GI), with most studies reporting 12-week follow-up. No consistent differences were found between ozone and CHX in terms of PPD, CAL, PI or BoP. No adverse effects were reported in the studies.</p><p><strong>Conclusions: </strong>Ozonated gels and oils were associated with favorable periodontal outcomes, particularly when used as adjuncts to subgingival instrumentation, and no major adverse effects were reported. However, the evidence is limited by short-term follow-up and methodological heterogeneity.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70439"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13506133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Evaluation of the Antifungal Activity of Salvia Leriifolia Leaf Hydroalcoholic Extract Compared to Nystatin and Fluconazole Against Candida albicans.","authors":"Anahita Ghorbani, Negareh Salehabadi, Mina Ramezani, Iman Haghani, Majid Saeedi, Abolfazl Hosseinnataj, Maede Salehi","doi":"10.1002/cre2.70436","DOIUrl":"10.1002/cre2.70436","url":null,"abstract":"<p><strong>Objectives: </strong>With the rising incidence of fungal infections in recent years, Candida albicans has become the leading cause of oral fungal infections, ranging from superficial to systemic forms. The extensive use of antifungal medications has led to drug resistance and adverse effects, highlighting the growing need to explore medicinal plants. This study aimed to assess the antifungal activity of the hydroalcoholic extract of Salvia leriifolia leaves on laboratory strains of Candida albicans, comparing its effects with those of the standard antifungal drugs Nystatin and Fluconazole.</p><p><strong>Material and methods: </strong>This experimental study used 20 confirmed Candida albicans isolates. Malt extract agar and RPMI-1640 media were prepared according to standard protocols, and Salvia leriifolia extract was obtained via maceration. Fungal suspensions and stock solutions were prepared at defined concentrations, and MICs were determined using the microdilution broth method per CLSI-M27-A3 guidelines. Data were analyzed with SPSS.</p><p><strong>Results: </strong>Salvia leriifolia extract demonstrated antifungal activity against Candida albicans, with MIC values ranging from 500 to 1000 µg/mL and a geometric mean MIC of 901.3 µg/mL. In contrast, nystatin and fluconazole exhibited markedly lower MIC ranges (0.063-16 and 0.063-1 µg/mL, respectively) and geometric mean MICs of 0.297 and 0.308 µg/mL, respectively. Statistical analysis revealed significantly higher MIC values for the extract compared with both antifungal agents (p < 0.001, effect size = 0.87), whereas no significant difference was observed between nystatin and fluconazole (p = 0.638). Despite its lower potency, the extract showed moderate antifungal activity against Candida albicans.</p><p><strong>Conclusions: </strong>This study demonstrated that while the hydroalcoholic extract of Salvia leriifolia is less potent than nystatin and fluconazole, it is still capable of partially inhibiting the growth of Candida albicans. These results underscore the potential role of medicinal plants in the treatment of fungal infections, especially in cases involving drug resistance or undesirable side effects from conventional medications.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70436"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13490917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Body Roundness Index and Periodontitis in Adults With Hypertension: A Cross-Sectional Study.","authors":"Liting Xie, Xiaobin Zhang, Zhiqiang Xu, Zhiyong Pan","doi":"10.1002/cre2.70429","DOIUrl":"10.1002/cre2.70429","url":null,"abstract":"<p><strong>Objectives: </strong>The association between body roundness index (BRI) and periodontitis among US adults with hypertension remains unclear. This study aimed to investigate the relationship between BRI and periodontitis in this population.</p><p><strong>Material and methods: </strong>This cross-sectional study included 4117 adults with hypertension from the National Health and Nutrition Examination Survey (NHANES) 2009-2014. Multivariable logistic regression models were used to assess the association between BRI and periodontitis. Generalized additive models and two-piecewise regression models were applied to explore potential nonlinearity and threshold effects. Stratified analyses across covariate-defined subgroups and sensitivity analyses were conducted to evaluate the robustness of the findings.</p><p><strong>Results: </strong>After adjustment for covariates, BRI was significantly associated with periodontitis. When BRI was analyzed by quartiles, participants in the highest quartile had significantly higher odds of periodontitis than those in the lowest quartile in both Model I (OR = 1.27, 95% CI: 1.05-1.54, p = 0.0122) and Model II (OR = 1.25, 95% CI: 1.03-1.53, p = 0.0258). A nonlinear association was observed between BRI and periodontitis. The log-likelihood ratio test was statistically significant (p < 0.05), and the inflection point was estimated to be approximately 6. At or above this point, the OR for the association between BRI and periodontitis was 1.09 (95% CI: 1.04-1.15, p = 0.0006), whereas below this point, the corresponding OR was 0.94 (95% CI: 0.87-1.02, p = 0.1209). Sensitivity analyses yielded results that were broadly consistent with the primary findings.</p><p><strong>Conclusions: </strong>Among US adults with hypertension, BRI was associated with periodontitis, and the association was nonlinear.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70429"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13472514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Diagnostic Performance of Cone Beam Computed Tomography and Dual-Energy X-Ray Absorptiometry for Low Bone Density Assessment. A Systematic Review and Meta-Analysis","authors":"Mohammad Alrashidi,&nbsp;Haitham Elbishari,&nbsp;May Aljanahi,&nbsp;Fatemeh Amir-Rad,&nbsp;Jahanzeb Chaudhry,&nbsp;Momen Atieh","doi":"10.1002/cre2.70423","DOIUrl":"https://doi.org/10.1002/cre2.70423","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This systematic review evaluated the diagnostic accuracy of CBCT for BMD assessment using DEXA as the reference standard and aimed to quantify its performance through meta-analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This review included prospective and retrospective observational studies assessing CBCT's diagnostic performance in detecting low BMD, with DEXA as the reference. Searches in PubMed, Embase, and Scopus identified 13 eligible studies involving adult participants (≥ 18 years). Two reviewers independently extracted data and assessed study quality using the QUADAS-2 tool. Meta-analysis was conducted using random-effects models.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Pooled sensitivity and specificity of CBCT for detecting low BMD were 0.58 (95% CI: 0.50–0.67) and 0.78 (95% CI: 0.69–0.86), respectively. The summary receiver operating characteristic (SROC) curve showed an area under the curve (AUC) of 0.73 (95% CI: 0.69–0.77), indicating moderate diagnostic accuracy. Subgroup analyses revealed heterogeneity influenced by sample size and anatomical site.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>CBCT demonstrates moderate diagnostic accuracy in detecting low BMD, with better specificity than sensitivity. While promising, standardization of CBCT protocols and larger studies are needed to confirm its role in clinical assessment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> PROSPERO Registration Number</h3>\u0000 \u0000 <p>(CRD42024598958).</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148647784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel FAM83H Truncation Mutation Disrupts Enamel Mineralization in Autosomal Dominant Hypocalcified Amelogenesis Imperfecta.","authors":"Yue Wang, Hongfei Chen, Jiyong Lai, Xueqing Huang, Yanyu Huang","doi":"10.1002/cre2.70431","DOIUrl":"10.1002/cre2.70431","url":null,"abstract":"<p><strong>Objectives: </strong>Autosomal dominant hypocalcified amelogenesis imperfecta (ADHCAI; OMIM#130900) is a hereditary enamel defect caused by truncation mutations in FAM83H, though the underlying pathogenic mechanisms remain incompletely understood. This study aimed to identify novel FAM83H mutations in a Chinese family with ADHCAI and to investigate their functional consequences on enamel formation.</p><p><strong>Materials and methods: </strong>A three-generation ADHCAI family underwent clinical and genetic evaluation. Whole-exome sequencing and Sanger sequencing were used for mutation identification. The proband's enamel was analyzed by scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX). Structural impacts of the mutation were predicted bioinformatically (PSIPRED, SWISS-MODEL). Patient-derived periodontal ligament cells (PDLCs) were assessed by Western blot, immunofluorescence, and quantitative real-time PCR for key enamel matrix proteins (AMELX, AMBN, ENAM) and osteogenic markers (RUNX2, ALP).</p><p><strong>Results: </strong>A novel heterozygous nonsense mutation (c.1819G>T, p.(Glu607*)) in FAM83H was identified, resulting in a C-terminally truncated protein lacking 573 residues that mislocalizes from the cytoplasm to the nucleus. SEM-EDX revealed disorganized enamel ultrastructure, a reduced calcium-to-phosphorus ratio, and increased porosity. Functional analysis in PDLCs showed coordinated downregulation of enamel matrix proteins and osteogenic markers, indicating a dual regulatory role for FAM83H in biomineralization.</p><p><strong>Conclusions: </strong>Our findings are consistent with the \"presecretory pathogenesis\" model for ADHCAI, wherein nuclear-mislocalized truncated FAM83H may disrupt enamel mineralization partly through transcriptional suppression of matrix components. This expands the FAM83H mutational spectrum, offers preliminary mechanistic insight, and suggests potential targets for pre-eruptive therapeutic strategies.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70431"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13472511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148763948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the Clinical Application Value of Immune Function Markers and Cytokines in the Recurrent Aphthous Ulcer.","authors":"Yiwen Chen, Ting Meng, Lahong Zhang, Qiang Ke","doi":"10.1002/cre2.70418","DOIUrl":"10.1002/cre2.70418","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to analyze the alterations in cellular and humoral immune functions, alongside cytokine profiles, in patients with recurrent aphthous ulcer (RAU) compared to those with other oral mucosal diseases and healthy controls (HC), and to investigate their clinical significance.</p><p><strong>Methods: </strong>Anticoagulated whole blood and serum samples were collected from 101 cases of the RAU group, the \"Others\" group, and the HC group. The percentages of lymphocyte subsets, including NKT, NK, CD4⁺T, CD8⁺T, and CD3^-CD19⁺B cells, were detected by flow cytometry. The concentrations of IgG, IgA, IgM, IgE, C3, and C4 were measured using immunoturbidimetry. Additionally, the levels of cytokines IL-6, IL-8, and TNF-α were quantified by chemiluminescence. Statistical analyses were performed to compare the clinical value of these functional markers across the different groups.</p><p><strong>Results: </strong>Non-parametric tests revealed that the percentage of NKT cells in the RAU group was significantly higher than those in both the \"Others\" and HC groups; furthermore, the \"Others\" group showed significantly higher levels than the HC group. The content of IgE in the RAU group was significantly elevated compared to the \"Others\" and HC groups. The levels of IL-6, IL-8, and TNF-α in the RAU group were significantly higher than those in the HC group. ROC analysis revealed that NKT cells, IgE, IL-6, and IL-8 showed AUC values greater than 0.7 in the diagnosis of RAU.</p><p><strong>Conclusion: </strong>This study demonstrates that the combined detection of NKT cells, IgE, and cytokines (IL-6, IL-8, and TNF-α) may potentially hold significant clinical application value in the assessment of RAU and other oral mucosal diseases.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70418"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13393289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping Performance of XP4 versus ProTaper Next in C-Shaped Canals: A Micro-CT Study of 3D-Printed Mandibular Second Molar Replicas.","authors":"Zaidao Xiong, Lu Wang, Wenyuan Zhou, Zhongyang Shan, Chao Liu, Yongchun Gu, Jia Li","doi":"10.1002/cre2.70434","DOIUrl":"10.1002/cre2.70434","url":null,"abstract":"<p><strong>Introduction: </strong>This micro-computed tomography (micro-CT) study aimed to evaluate the shaping ability of two nickel-titanium (NiTi) rotary systems-XP4 and ProTaper Next (PTN)-in 3D-printed replicas of C-shaped canals.</p><p><strong>Methods: </strong>Two extracted mandibular second molars with C-shaped root canals-one continuous (Melton's Category I) and one discontinuous (Melton's Category II/III)-were selected and replicated via 3D printing. Replica accuracy was validated by best‑fit alignment. Sixteen resin replicas per specimen were randomly assigned to PTN or XP4 instrumentation (n = 8 per group) following the manufacturers' protocols. Pre- and post-instrumentation micro-CT scans were performed. Three-dimensional registration and reconstruction enabled quantitative analysis of (1) volume/surface area changes and (2) unprepared canal surfaces.</p><p><strong>Results: </strong>The replicas demonstrated high fabrication fidelity. In continuous C-shaped canals, PTN removed significantly less resin (2.7 mm³, 7.4% of initial volume) than XP4 (4.3 mm³, 11.6%; p < 0.01). Conversely, in discontinuous C-shaped configurations, PTN exhibited greater removal (13.0 mm³, 23.1%) versus XP4 (8.9 mm³, 15.8%; p < 0.01). Unprepared canal surface in continuous C replicas was higher with PTN (42.0%) than XP4 (25.5%; p < 0.01), whereas no significant difference was observed in discontinuous C replicas (PTN: 32.0%, XP4: 31.6%; p > 0.05). Procedural complications were observed exclusively in discontinuous C-shaped canals, with PTN causing two cases of critical wall thinning and XP4 exhibiting two instrument separations.</p><p><strong>Conclusion: </strong>In continuous C-shaped canal replicas, XP4 showed superior shaping capability with significantly lower percentages of unprepared surface compared to PTN. In discontinuous C-shaped configurations, both systems achieved comparable unprepared surface areas, although they differed markedly in resin removal patterns and iatrogenic risks. These results indicate that the relative performance and safety profiles of NiTi systems may vary depending on specific C-shaped canal anatomy. Clinicians should consider canal configuration, curvature severity, and dentin thickness when selecting instruments for C-shaped canal instrumentation.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70434"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13472515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148764313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fracture Resistance of Thermal-Mechanical Aged Resin Composite-Restored Premolars: Effect of Different Application Protocols of a Universal Adhesive.","authors":"Fereshteh Shafiei, Marzieh Moradian, Marziyeh Naseri Mojarad","doi":"10.1002/cre2.70424","DOIUrl":"10.1002/cre2.70424","url":null,"abstract":"<p><strong>Objectives: </strong>This research investigated the effect of different applications of Universal bonding on the fracture resistance (FR) of maxillary premolars restored with mesio-occluso-distal resin composite.</p><p><strong>Materials and methods: </strong>One-hundred and eight extracted maxillary premolars were randomly assigned into nine groups (n = 12): (1) ST, sound teeth; (2) nonrestored teeth; (3) SEE, selective enamel etching; (4) E&R, etch-and-rinse; (5) SDE, selective dentin etching; (6) SEE+resin layer; (7) E&R+resin layer; (8) SDE+resin layer/precuring; (9) SDE+resin layer/co-curing. Following restoration, mechanical loading (100,000 cycles, 50 N), 6-month water storage, and thermocycling (1000 cycles), FR was tested. Failure mode was evaluated. Data were analyzed using one-way ANOVA and Tukey test (α = 0.05).</p><p><strong>Results: </strong>SDE+resin layer/precuring showed the highest mean fracture load (1222.0 ± 58.7 N), with a significant difference with all groups (p < 0.001), except SDE+resin layer/co-curing (1166.5 ± 50.3 N). FR of SEE with the lowest value (894.50 ± 70.41 N) was lower than SDE (p < 0.001) but comparable with E&R. Resin layer significantly improved FR in all etching conditions, except the E&R group (p = 0.272). The highest number of restorable fractures was in two SDE with resin layer groups.</p><p><strong>Conclusion: </strong>Adhesive application protocols affected FR. Combining short-time dentin etching with a HEMA-free hydrophobic resin may be suggested to increase the stable reinforcement potential of universal adhesive-resin composite restorations in premolars and preserve teeth under high loading.</p><p><strong>Clinical relevance: </strong>The adhesive application protocol considerably affected the fracture resistance of MOD resin composite-restored premolars. A short time dentin etching using an ultra-mild universal adhesive, along with increasing hydrophobicity of the adhesive interface, may enhance the mechanical reinforcement of resin composite-restored premolars during simulated thermal-mechanical intraoral conditions. This approach also possibly prevents the risk of catastrophic failure under unexpectedly high loads.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70424"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13490923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood Pressure and Systemic Risk Factors in Patients Presenting With Post-Extraction and Periodontal Bleeding at a Dental Out-of-Hours Clinic.","authors":"Yosuke Iijima, Miki Yamada, Shunsuke Hino, Motohiko Sano, Norio Horie, Takahiro Kaneko","doi":"10.1002/cre2.70446","DOIUrl":"10.1002/cre2.70446","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to verify whether blood pressure was higher in patients presenting to the dental out-of-hours clinic with post-extraction bleeding (PEB) and periodontal disease-related gingival bleeding (PDB) compared to those presenting for other conditions (Control (C) group). Additionally, we examined differences in the prevalence of hypertension, antithrombotic use, and diabetes mellitus among the PEB, PDB, and C groups.</p><p><strong>Materials and methods: </strong>Patients visiting the out-of-hours clinic were retrospectively categorized by sex and age (young (Y)-group: 20-39, middle (M)-group: 40-59, older (O)-group: ≥ 60). Data collected included systolic (SBP) and diastolic (DBP) blood pressure, and the prevalence of treated hypertension, antithrombotic use, and diabetes mellitus. Mean BP values were compared using Welch's t-test. The prevalence of systemic factors was compared using Fisher's exact test. Multivariable logistic regression analyses were performed in the O-group.</p><p><strong>Results: </strong>A total of 416 bleeding patients were included, comprising 216 patients with PEB, 119 with PDB, and 81 with other bleeding. Because of the limited number of cases in the Y and M-groups, multivariable logistic regression analyses were performed only in the O-group (≥ 60 years). According to that, antithrombotic use was independently associated with both PDB and PEB in males and females. Increased SBP was independently associated with PDB in both sexes and with PEB in males. Hypertension remained independently associated with PDB only in females, whereas diabetes mellitus was not independently associated with either bleeding condition.</p><p><strong>Conclusion: </strong>Particularly among the O-group (≥ 60 years), antithrombotic use was independently associated with both PDB and PEB, whereas increased systolic blood pressure was independently associated with PDB and, to a lesser extent, PEB. These findings highlight the importance of systemic assessment, particularly regarding blood pressure and antithrombotic therapy, in patients presenting with acute oral bleeding.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":"e70446"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13519035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148825763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}