Time-Dependent Changes in Effects of Butyrate on Human Gingival Fibroblasts

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2025-04-24 DOI:10.1002/cre2.70120

Haruki Otani, Jumpei Washio, Aoi Kunitomi, Satoko Sato, Yuki Abiko, Shiori Sasaki, Kazumasa Ohashi, Satoru Yamada, Nobuhiro Takahashi

{"title":"Time-Dependent Changes in Effects of Butyrate on Human Gingival Fibroblasts","authors":"Haruki Otani, Jumpei Washio, Aoi Kunitomi, Satoko Sato, Yuki Abiko, Shiori Sasaki, Kazumasa Ohashi, Satoru Yamada, Nobuhiro Takahashi","doi":"10.1002/cre2.70120","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>Butyrate is one of major metabolites of periodontitis-associated bacteria and often detected in periodontal pockets. Butyrate has been considered to affect human gingival fibroblasts (HGFs); however, there was no information on its long-term effect as occurs in periodontitis. Therefore, this study aimed to evaluate the time-dependent effects of butyrate on HGFs.</p>\n </section>\n \n <section>\n \n <h3> Material and Methods</h3>\n \n <p>The effects of butyrate on HGF proliferation, apoptosis, cell morphology, glucose metabolic activity, butyrate metabolic activity, and cell migration ability were evaluated by cell counting, DNA electrophoresis, cell staining, pH-stat system, HPLC, and scratch test, respectively.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>HGF proliferation was temporarily inhibited by 5–10 mM butyrate (<i>p</i> < 0.05); however, it resumed at 24 h with morphological changes from spindle to slightly widened (<i>p</i> < 0.05). HGFs cultured with 10 mM butyrate for 12–24 h shifted the glucose metabolic pathway from oxidative phosphorylation to glycolysis (<i>p</i> < 0.05), and increased butyrate consumption, which returned to control levels over 24 h. HGF migration ability tended to decrease at 72 h.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>HGF cell proliferation and glucose/butyrate metabolism were temporarily inhibited by butyrate and then recovered in a time-dependent manner, accompanied by changes in cell morphology. These time-dependent effects may help to understand the role of butyrate in the pathology of periodontitis.</p>\n </section>\n </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70120","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Dental Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cre2.70120","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

Butyrate is one of major metabolites of periodontitis-associated bacteria and often detected in periodontal pockets. Butyrate has been considered to affect human gingival fibroblasts (HGFs); however, there was no information on its long-term effect as occurs in periodontitis. Therefore, this study aimed to evaluate the time-dependent effects of butyrate on HGFs.

Material and Methods

The effects of butyrate on HGF proliferation, apoptosis, cell morphology, glucose metabolic activity, butyrate metabolic activity, and cell migration ability were evaluated by cell counting, DNA electrophoresis, cell staining, pH-stat system, HPLC, and scratch test, respectively.

Results

HGF proliferation was temporarily inhibited by 5–10 mM butyrate (p < 0.05); however, it resumed at 24 h with morphological changes from spindle to slightly widened (p < 0.05). HGFs cultured with 10 mM butyrate for 12–24 h shifted the glucose metabolic pathway from oxidative phosphorylation to glycolysis (p < 0.05), and increased butyrate consumption, which returned to control levels over 24 h. HGF migration ability tended to decrease at 72 h.

Conclusions

HGF cell proliferation and glucose/butyrate metabolism were temporarily inhibited by butyrate and then recovered in a time-dependent manner, accompanied by changes in cell morphology. These time-dependent effects may help to understand the role of butyrate in the pathology of periodontitis.

Abstract Image

查看原文本刊更多论文

丁酸盐对人牙龈成纤维细胞影响的时间依赖性变化

目的丁酸盐是牙周炎相关细菌的主要代谢物之一，常见于牙周袋。丁酸盐被认为会影响人牙龈成纤维细胞（HGFs）；然而，尚无关于其在牙周炎中的长期效果的信息。因此，本研究旨在评估丁酸盐对hgf的时间依赖性影响。材料与方法分别通过细胞计数、DNA电泳、细胞染色、pH-stat系统、HPLC和划痕试验评价丁酸盐对HGF增殖、凋亡、细胞形态、葡萄糖代谢活性、丁酸盐代谢活性和细胞迁移能力的影响。结果5 ~ 10 mM丁酸盐对HGF增殖有短暂抑制作用（p < 0.05）；24 h后恢复，形态由纺锤体略微变宽（p < 0.05）。用10 mM丁酸盐培养hgf 12-24 h后，葡萄糖代谢途径从氧化磷酸化转变为糖酵解（p < 0.05），并增加丁酸盐消耗，24 h后恢复到对照水平。HGF的迁移能力在72 h时呈下降趋势。结论丁酸盐对HGF细胞增殖和葡萄糖/丁酸代谢有短暂抑制作用，抑制后恢复并伴有细胞形态的改变。这些时间依赖性效应可能有助于理解丁酸盐在牙周炎病理中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.