Clinical and Experimental Dental Research最新文献

{"title":"In Vitro Evaluation of Flexural Strength, Impact Strength, and Surface Microhardness of Vaccinium macrocarpon Reinforced Polymethyl Methacrylate Denture Base Resin","authors":"Anitha Kuttae Viswanathan,&nbsp;Rajkumar Krishnan","doi":"10.1002/cre2.70145","DOIUrl":"https://doi.org/10.1002/cre2.70145","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The antimicrobial efficacy of cranberry (CA) against oral infections was well evidenced. Influence of cranberry on the mechanical properties of heat-activated polymethyl methacrylate (HA-PMMA) denture base resin (DBR) is unexplored till date and is significant for a better understanding of the reinforcement. This study aimed to evaluate the flexural strength (FS), impact strength (IS), and surface microhardness (VHN) of heat-cure PMMA DBR reinforced with varying concentrations of <i>Vaccinium macrocarpon</i> (cranberry) extract.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A total of 150 samples were categorized into five groups (<i>n</i> = 10) by weight percentage of 0, 0.5, 1.0, 1.5, and 2.0 cranberry extract added into HA-PMMA polymer after the performance of antimicrobial efficacy testing of CA. Three-point bending test for FT, Izod impact testing for IS, and Vickers microhardness test were performed. Fractured sample surface was characterized by a high-resolution scanning electron microscope (HR-SEM). Raw data were statistically analyzed with one-way ANOVA and post hoc Bonferroni test.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A significant improvement in flexural strength of 76.88 ± 0.73 MPa, impact strength of 6.66 ± 0.24 kJ/m², and microhardness of 18.44 ± 0.27 kg/mm² was observed at 2 wt.% (<i>p</i> &lt; 0.0001). Fractured surface topography showed dispersion of cranberry particles as a thin fibrous band intermeshed within resin matrix.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Addition of up to 2 wt.% cranberry improved the FS, IS, and VHN on comparison to 0 wt.% control HA-PMMA.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Methylation of iNOS, eNOS, TP53 and LINE-1 in Gingival Tissues of Cigarette Smokers, Heat-Not-Burn Smokers and Never Smokers: An Exploratory Study","authors":"Giulio Rasperini,&nbsp;Michele Codari,&nbsp;Alessandra Moiana,&nbsp;Letizia Tarantini,&nbsp;Valentina Bollati,&nbsp;Gionata Bellucci,&nbsp;Dario Consonni,&nbsp;Lena Larsson,&nbsp;Farah Asa'ad","doi":"10.1002/cre2.70141","DOIUrl":"https://doi.org/10.1002/cre2.70141","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>In this exploratory study, the influence of traditional cigarette smoking and heat-not-burn device smoking (HnB) on DNA methylation of cancer-associated genes in smokers compared to never smokers was evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials &amp; Methods</h3>\u0000 \u0000 <p>Twenty-eight healthy individuals undergoing dental care were enrolled into three groups: (i) 10 traditional smokers, (ii) 8 HnB smokers, and (iii) 10 never smokers. Gingival biopsies were obtained, and DNA methylation analysis was performed by pyrosequencing for the cancer-associated genes: <i>iNOS</i>, <i>eNOS</i> and <i>TP53</i>. LINE-1 sequence was selected for genome-wide methylation readout.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>HnB smokers exhibited approximately a 13% higher <i>iNOS</i> methylation compared to both traditional smokers and never smokers (<i>p</i> = 0.004 in a crude analysis and <i>p</i> = 0.01 in a multivariable linear regression model adjusted for gender and age). However, HnB smoking did not influence the DNA methylation levels of <i>TP53, eNOS</i>, and LINE-1 in gingival tissues, as these were comparable to the levels observed in traditional smokers and never smokers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>HnB device smoking increased DNA methylation levels of <i>iNOS</i> gene, which might indicate a decreased iNOS expression in HnB smokers, compared to traditional smokers and never smokers. Implications of the observed <i>iNOS</i> methylation status in the development of oral cancer needs to be investigated in future studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peri-Implant Diseases: Enhanced Antibacterial Photodynamic Therapy","authors":"Hanna Lähteenmäki,&nbsp;Tommi Pätilä,&nbsp;Ismo T. Räisänen,&nbsp;Rauni Kalliala,&nbsp;Timo Sorsa","doi":"10.1002/cre2.70146","DOIUrl":"https://doi.org/10.1002/cre2.70146","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The number of dental implants is constantly on the rise, and inflammation of their surrounding tissues is an increasing problem. In this randomized controlled trial, we investigated the effects of antibacterial dual-light photodynamic therapy (aPDT) in peri-implant disease in reduction of inflammation in the peri-implant tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Forty patients with newly diagnosed peri-implant disease were recruited. The diagnoses were established clinically and by radiological bone loss (RBL). Dual-light aPDT was provided by indocyanine green mouth rinsing and 50:50, 405 nm, and 810 nm light applicator. The treatment group (<i>n</i> = 20) was randomized to use aPDT treatment at home for 4 weeks. The treatment was carried out once daily for 2 weeks, then twice daily for the next 2 weeks. The control group (<i>n</i> = 20) continued enhanced self-care during the study period. Traditional measurement methods around the implant were measured at the beginning, at 2 weeks and at 4 weeks.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>During the study period, VPI decreased in both groups. In the treatment group BOP decreased from mean (SD) 4.7 ± 1.3 to 1.8 ± 1.6, <i>p</i> &lt; 0.0001, but no change was observed in the control group (3.5 ± 2.3 to 3.0 ± 2.3, <i>p</i> = 0.39). In the aPDT treatment group aMMP-8 decreased from mean (SD) 100 ± 41 to 72 ± 38, <i>p</i> = 0.027, but not in the control group (86 ± 54 to 86 ± 60, <i>p</i> = 0.38).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Regularly applied dual-light aPDT reduces inflammation in the dental implant adjacent tissues. Dual-light aPDT approach holds promise as an effective home care tool for implant patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positional Accuracy of Dental Implants Placed by Means of Fully Guided Technique in Partially Edentulous Patients: A Retrospective Study","authors":"Mariano Tia,&nbsp;Alessia Teresa Guerriero,&nbsp;Antonio Carnevale,&nbsp;Ilaria Fioretti,&nbsp;Gianrico Spagnuolo,&nbsp;Gilberto Sammartino,&nbsp;Roberta Gasparro","doi":"10.1002/cre2.70144","DOIUrl":"https://doi.org/10.1002/cre2.70144","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Computer-aided implant surgery (CAIS) is a fully digital approach that guides the biological and prosthetic ideal implant position. The aim of this retrospective clinical study was to assess the accuracy of implant position using CAIS and clinical outcomes, in partially edentulous patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>This study was designed as retrospective study. Twenty-one patients requiring a maximum of two implants were recruited from 2023 to 2024 at the University of Naples Federico II. For all patients, 3D cone-beam computed tomography (CBCT) and intraoral scans were obtained and superimposed by matching the resulting DICOM and STL data files in a software to create the tooth-supported surgical guide. All implants were placed using a fully guided implant surgery protocol. The accuracy of the technique was measured by the deviation between the actual implant position (mesio-distal deviation, depth error, and axis deviation) obtained from the postoperative CBCT and the preoperative planned implant position. Clinical outcomes assessed included biological complications, implant and prosthetic failures, esthetic outcomes, and patient satisfaction. Descriptive analysis was performed using mean and standard deviation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 37 implants were analyzed. The mean results were as follows: 0.43 ± 0.20 mm of mesio-distal linear deviation at the implant shoulder, 0.24 ± 0.07 mm of depth error, and 1.46° ± 0.31° of axis deviation. At 6 months, healing was uneventful for all patients, with no complications or implant or prosthetic failures reported. Patients with implant-supported restorations expressed high levels of functional and esthetic satisfaction.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The fully guided technique achieved clinically acceptable accuracy positioning of dental implants in partially edentulous patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes of the Occlusal Relationship in Patients With Temporomandibular Disorders (TMD) After Manual Therapy: A Pilot Study","authors":"Tobias Dieter Peter Pohl,&nbsp;Alessia Celine Harhoff,&nbsp;Johannes Ries,&nbsp;Manfred Wichmann,&nbsp;Ragai-Edward Matta","doi":"10.1002/cre2.70147","DOIUrl":"https://doi.org/10.1002/cre2.70147","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Objectives&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Temporomandibular disorders (TMD) are often linked to changes in dental occlusion, yet the impact of therapeutic interventions remains unclear. This pilot study investigates the effects of manual therapy on occlusion in TMD patients through quantitative analysis of intraoral scans.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Material and Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Ten individuals were diagnosed with TMD and underwent a 40-min session of manual therapy (Group MT). Ten subjects were allocated to the healthy control group (Group C) and did not receive any therapy during the 10-week control period. Occlusion measurements were obtained using the TRIOS 3 intraoral scanner (3Shape, Denmark) both before and after the therapy or control period. The digital models were analyzed regarding occlusal changes using the GOM Inspect Professional software (GOM, Germany). The differences in the individual axes &lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 \u0000 &lt;mrow&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;dX&lt;/mi&gt;\u0000 \u0000 &lt;mi&gt;Pat&lt;/mi&gt;\u0000 &lt;/msub&gt;\u0000 \u0000 &lt;mo&gt;,&lt;/mo&gt;\u0000 \u0000 &lt;msub&gt;\u0000 &lt;mi&gt;dY&lt;/mi&gt;\u0000 \u0000 &lt;mi&gt;Pat&lt;/mi&gt;\u0000 &lt;/msub&gt;\u0000 \u0000 &lt;mo&gt;,&lt;/mo&gt;\u0000 \u0000 &lt;msub&gt;\u0000 &lt;mi&gt;dZ&lt;/mi&gt;\u0000 \u0000 &lt;mi&gt;Pat&lt;/mi&gt;\u0000 &lt;/msub&gt;\u0000 \u0000 &lt;mo&gt; &lt;/mo&gt;\u0000 \u0000 &lt;mrow&gt;\u0000 &lt;mo&gt;(&lt;/mo&gt;\u0000 \u0000 &lt;mi&gt;mm&lt;/mi&gt;\u0000 \u0000 &lt;mo&gt;)&lt;/mo&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;annotation&gt; ${{dX}}_{{Pat}},{{dY}}_{{Pat}},{{dZ}}_{{Pat}}{unicode{x02007}}(mathrm{mm})$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt; and Euclidean distance &lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 \u0000 &lt;mrow&gt;\u0000 &lt;msub&gt;\u0000 &lt;mrow&gt;\u0000 &lt;mi&gt;d&lt;/mi&gt;\u0000 ","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond Accuracy: Clinical Outcomes of Computer Assisted Implant Surgery","authors":"Sofya Sadilina,&nbsp;Kay Vietor,&nbsp;Romain Doliveux,&nbsp;Adam Siu,&nbsp;Zhuofan Chen,&nbsp;Bilal Al-Nawas,&nbsp;Nikos Mattheos,&nbsp;Allesandro Pozzi","doi":"10.1002/cre2.70129","DOIUrl":"https://doi.org/10.1002/cre2.70129","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Computer Assisted Implant Surgery (CAIS) with different technologies and modalities is becoming increasingly utilized in clinical practice. The aim of this White Paper was to synthesize evidence, reported experience, and best practices with regard to clinically relevant outcomes of static, dynamic, and robotic CAIS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A review of the literature compiled existing evidence from clinical studies up to November 2024, which was later discussed and synthesized into clinically relevant questions with a panel of international experts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There is overwhelming evidence for the superiority of static, dynamic, and robotic CAIS with regard to the accuracy of implant placement and some limited evidence of superior esthetic outcomes. At the same time, outcomes related to implant primary stability, survival rates, intra- and postoperative complications, marginal bone loss, and peri-implant tissue health appear similar between guided and non-guided implant surgery, while efficiency is poorly defined and studied. The importance of accuracy in the execution of a comprehensive, prosthetically driven treatment plan is not reflected in most studies, which focus mainly on the assessment of procedures rather than entire treatment workflows. Such inherent limitations of available research might conceal some of the potential of guided CAIS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Guided CAIS can achieve at least as good clinical outcomes as non-guided implant surgery. Studies that can assess the benefits of CAIS as part of a treatment workflow, rather than isolated procedures, could improve our understanding of the potential of these technologies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Assessment of Salivary Pellicle Disruption and Biofilm Removal on Titanium: Exploring the Role of Surface Hydrophobicity in Chemical Disinfection","authors":"Wenji Cai,&nbsp;Azam Fayezi Sisi,&nbsp;Mohamed-Nur Abdallah,&nbsp;Ashwaq A. Al-Hashedi,&nbsp;Juan Daniel Gamonal Sánchez,&nbsp;Enrique Bravo,&nbsp;Hasna H. Kunhipurayil,&nbsp;Rubens Albuquerque,&nbsp;Zahi Badran,&nbsp;Mariano Sanz,&nbsp;Faleh Tamimi","doi":"10.1002/cre2.70082","DOIUrl":"https://doi.org/10.1002/cre2.70082","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Peri-implantitis is mostly caused by a pathological biofilm that forms through complex processes, initiated by the formation of the salivary pellicle on implant surfaces. Understanding the nature of these pellicles and biofilm and how to remove them is important for preventing peri-implant infections and improving the success of dental implants. This study explores the characteristics of the salivary pellicle on titanium surfaces and assesses the effectiveness of different decontamination agents in eliminating the salivary pellicle and related microbial contaminations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Titanium surfaces were contaminated with salivary pellicles and pathological biofilms. The nature of the salivary pellicle was characterized using X-ray photoelectron spectroscopy (XPS), surface proteomics, contact angle measurements, and fluorescence microscopy. We tested six commonly used decontamination chemicals (chlorhexidine, essential oil-based mouthwash, citric acid, phosphoric acid, saline, and phosphate buffer saline) as well as newly proposed treatments such as surfactants and solvents (acetone, acetic acid, and Tween 20) for their capability to eliminate salivary pellicles and pathogenic biofilms from titanium surfaces.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The hydrophobic nature of the salivary pellicle on titanium surfaces limits the efficacy of commonly used hydrophilic solutions in removing pellicles and bacteria. Organic solvents and surfactants, particularly acetic acid and Tween 20, demonstrated superior effectiveness in removing the pellicle and biofilm. Acetic acid was notably effective in restoring surface composition, reducing microbial levels, and removing multispecies biofilms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The use of surfactants and solvents could be a promising alternative for the treatment of biofilms on titanium surfaces. However, further studies are needed to explore their clinical applicability.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143926092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predisposing Factors for Erosive Tooth Wear in Permanent Teeth Among Asthmatic Children and Adolescents","authors":"Tomi Ujčič Samec,&nbsp;Janja Jan","doi":"10.1002/cre2.70142","DOIUrl":"https://doi.org/10.1002/cre2.70142","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Epidemiological studies indicate that erosive tooth wear (ETW) is a common threat of tooth surface loss. The etiology of ETW is multifactorial. The prevalence of asthma is increasing in developed countries, especially in children. Studies evaluating ETW in asthmatic children are conflicting. With our study, we aimed to investigate the association between general and asthmatic factors and the presence of ETW.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Population of this cross-sectional observational study consisted of children aged 6–17 years under treatment for asthma at University Medical Centre. ETW was determined using the Basic Erosive Wear Examination index. Questionnaires completed by parents and data from patients' medical records provided information on demographics, medical history, medication use, dietary habits, oral hygiene, fluoride exposure and type, dose, frequency, duration, and mode of asthma medication use.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>379 asthmatic children participated in the study. The prevalence of ETW was 17.2% (<i>n</i> = 379). The mean total BEWE score was 0.76 ± 2.12. A statistically significant higher presence of ETW and higher total BEWE index were found in the group consuming acidic sports drinks (OR = 3.318), in the group aged 12–17 years (OR = 6.233), in the group using asthma medication for more than 3 years (OR = 3.379) and in the group using medication in the dry powder inhaled form (OR = 2.447).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Asthmatic children should avoid drinking acidic drinks since longer duration of asthma medication use is already associating them with higher ETW presence. From the ETW point of view, metered-dose inhaled medications are more tooth-friendly than dry powder inhaled forms.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Let-7e as Potential Diagnostic Biomarkers in Early Detection of Peri-Implant Diseases: A Cross-Sectional Study","authors":"Nurul Latifah Zainal Abidin,&nbsp;Norjehan Latib,&nbsp;Fouad Hussain Al-Bayaty,&nbsp;Mohd Faizal Hafez Hidayat","doi":"10.1002/cre2.70044","DOIUrl":"https://doi.org/10.1002/cre2.70044","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Peri-implant diseases (PID), specifically peri-implant mucositis and peri-implantitis, significantly jeopardize dental implant success. Traditional diagnostic methods, relying on periodontal probing and radiographs, have limitations in precisely diagnosing these conditions. Recent studies suggest that microRNA (miRNA) profiling might be a breakthrough in the early detection of PID. This study aimed to evaluate the expression levels of specific miRNAs—miR-98, miR-145, miR-146a, and let-7e—in individuals with peri-implant mucositis and peri-implantitis compared to a healthy control.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>From January 2022 to October 2023, 19 participants with 38 dental implants were recruited according to established criteria. After clinical and radiographic evaluations, dental implants were grouped as peri-implant health (Group 1), peri-implant mucositis (Group 2), and peri-implantitis (Group 3). Subsequently, peri-implant crevicular fluid (PICF) samples were collected and analyzed through real-time quantitative polymerase chain reaction (qPCR).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Based on the findings, only let-7e biomarkers were consistently detected across all samples, with their expression increased eightfold in peri-implant mucositis and 94-fold in peri-implantitis cases. Kruskal–Wallis's test showed a statistically significant difference in relative gene expression of let-7e between the different groups, <i>H</i>(2) = 25.825, <i>p</i> ≤ 0.001.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our study indicates that miRNA biomarkers, particularly let-7e, may play a significant role in the development and progression of PID, highlighting the need for further in-depth investigation, with PICF offering a practical, noninvasive sampling method.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143909250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Structural, Biological, and Bone Induction Properties of Sol–Gel–Derived Lithium-Doped 68S Bioactive Glass—An in Vitro Study on Human Dental Pulp Stem Cells","authors":"Pejman Janbaz,&nbsp;Faeze Behzadpour,&nbsp;Kiana Ghanadan","doi":"10.1002/cre2.70139","DOIUrl":"https://doi.org/10.1002/cre2.70139","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Calcium silicate–based bioactive glass shows enhanced ion release capabilities and promotes the formation of hydroxyapatite (HA). This study aimed to synthesize a sol–gel–derived 68S bioactive glass (BAG) incorporating lithium (Li) and evaluate its structural, biological, and osteoinductive properties using human dental pulp stem cells (hDPSCs).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Two types of 68S BAG were synthesized using the sol–gel method: one containing 5 mol.% lithium nitrate (BGLi5) and a lithium-free control (BG). Structural characterization and HA formation were assessed using field emission scanning electron microscopy (FESEM) and Fourier-transform infrared spectroscopy (FTIR) before and after immersion in simulated body fluid (SBF) on Days 1, 3, and 7. The dissolution rates of the specimens were evaluated using inductively coupled plasma atomic emission spectroscopy (ICP-AES) and pH analysis. Biological activities were investigated through cell viability (MTT assay), alkaline phosphatase (ALP) enzyme activity, and alizarin red staining to assess mineralization. Additionally, the antimicrobial efficacy of the materials was tested against <i>Streptococcus mutans</i> (SM).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>FTIR and FESEM analyses confirmed the formation of HA crystals in BGLi5 specimens by Day 3 and in BG specimens by Day 7. The MTT assay demonstrated enhanced cell viability in both BG and BGLi5 compared to the control group. ALP activity, a marker of cell differentiation, was significantly elevated in the BGLi5-DM group by Day 14. Alizarin red staining on Day 21 revealed a marked increase in mineralization in both BG and BGLi5, with the BGLi5-DM group showing the highest mineralization levels. Furthermore, both BG and BGLi5 demonstrated significant antimicrobial activity against <i>SM</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The sol–gel–derived 68S BAG containing 5 mol.% Li is a biocompatible material that enhances cell proliferation, differentiation, and mineralization. The combination of BGLi5 with differentiation-specific culture medium synergistically promotes osteogenic differentiation and mineralization, making it a promising candidate for dental and bone tissue engineering applications.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 3","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}