Clinical and Experimental Dental Research最新文献

{"title":"Force Degradation of Intermaxillary Latex Elastics: Comparative In Vitro and In Vivo Study.","authors":"Lucie Ptáčková, Barbora Ličková, Wanda Urbanová, David Sluka, Klaudia Portašíková, Soňa Chamlarová, Iva Voborná, Ivana Dubovská","doi":"10.1002/cre2.70072","DOIUrl":"https://doi.org/10.1002/cre2.70072","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to compare the force degradation of intermaxillary elastics (IE) in vitro and in vivo while stretching the IE to a precise diameter.</p><p><strong>Materials and methods: </strong>IE 3/16″ medium Dentaurum from five different batches of packaging were analyzed. The in vivo study involved 10 volunteers, of which 100 IE were examined. To achieve three times the original diameter of the elastic, the distance between the upper canine and the lower dental arch was measured. Buttons were then placed in the mouth accordingly, and IE and passive aligners were inserted for five sessions of 48 h each. To investigate in vitro, 100 IE were placed in an incubator set at 37°C in a humid environment and stretched three times their diameter. The force of the elastics was measured in both investigations using a force meter at 0, 2, 8, 24, and 48 h.</p><p><strong>Results: </strong>In all patients except one, the three times diameter distance extended from the upper canine to the lower second premolar. The force degradation in vivo at 2, 8, 24, and 48 h was 20.58%, 26.78%, 34.81%, and 38.56% and in vitro was 16.38%, 22.83%, 28.32%, and 30.78%.</p><p><strong>Conclusions: </strong>The amount of stretching of IE varies for each patient when using standard insertion points. The force of IE decreases exponentially, the force degradation in vivo being higher. The clinician must consider the force decrease when advising the patient of the time interval to change the elastics.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":"e70072"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Multimodality Treatment Including Stabilization Splint and Low-Level Laser Therapies on Managing Temporomandibular Disorders: A Pilot Randomized Controlled Trial.","authors":"Zahra Afshari, Nazieh Abdollah Kookhi, Mahdi Shamali, Majid Sedaghat Monfared, Sara Tavakolizadeh","doi":"10.1002/cre2.70038","DOIUrl":"https://doi.org/10.1002/cre2.70038","url":null,"abstract":"<p><strong>Objectives: </strong>Temporomandibular disorder (TMD) encompasses various clinical issues affecting the temporomandibular joint, masticatory muscles, and surrounding structures. Common symptoms include pain, joint and muscle tenderness, and limited jaw movement. Diverse treatment options have been utilized to manage TMD. However, evidence of the combined modality treatment approach is scarce. This pilot trial aimed to evaluate the effectiveness of combining stabilization splint therapy (SST) with low-level laser therapy (LLLT) in managing TMD.</p><p><strong>Material and methods: </strong>This pilot parallel randomized clinical trial included 30 patients with TMD. Patients were randomly allocated equally into three treatment groups: SST, LLLT, and combined treatment (CT). Outcomes, including pain and functional limitations, were measured at baseline, 1, 2, 3, and 4 weeks after the start of each treatment.</p><p><strong>Results: </strong>CT was significantly more effective in reducing pain in patients with TMD compared to LLLT and SST. Although patients in the LLLT group reported significantly reduced pain, they still had higher pain scores compared to the SST group at certain time points, indicating that LLLT was not as effective as SST. Most functional limitations were improved over time, more consistently within the CT group, but without significant differences between the three groups. Patients were more satisfied with CT compared to LLLT and SST.</p><p><strong>Conclusions: </strong>The superior results of the CT group over the SST and LLLT groups were evident in this pilot trial's outcomes. The combined modality treatment approach seems to yield the greatest improvement for TMD patients.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":"e70038"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Occlusal Scheme and Bone-Level Implant Number and Position on Stress Distribution in Kennedy Class II Implant-Assisted Removable Partial Dentures: A 3D Finite Element Analysis.","authors":"Solmaz Barati, Safoura Ghodsi, Somayeh Zeighami","doi":"10.1002/cre2.70078","DOIUrl":"10.1002/cre2.70078","url":null,"abstract":"<p><strong>Objectives: </strong>To assess the effect of occlusion and implant number/position on stress distribution in Kennedy Class II implant-assisted removable partial denture (IARPD).</p><p><strong>Materials and methods: </strong>IARPDs were designed in six models: with one implant (bone level with a platform of 4 mm and length of 10 mm) at the site of (I) canine, (II) between first and second premolars, (III) first molar, (IV) second molar, or two implants at the sites of (V) canine-first molar, and (VI) canine-second molar. A conventional RPD served as control. Loads were applied according to the group function (GF) (500N load was applied to the left canine/premolar/molar teeth in the ratio of 1:1:2) or canine guidance (CG) (125N load was to the canine tooth) occlusions. Maximum displacement and Von Mises Stress in different components were analyzed by finite element analysis (FEA).</p><p><strong>Results: </strong>The control model showed the highest displacement followed by the IARPD with a canine implant in both occlusal schemes. In GF, the maximum and minimum jaw stress were recorded in IARPDs with canine implants (16.45 MPa) and canine-first molar implants (13.47 MPa), respectively. In CG, the maximum and minimum jaw stress was recorded in IARPD with first/second premolar implant (15.91 MPa) and canine-first molar implants (12.38 MPa), respectively. The highest stress in resin, framework, and implant(s) was noted in IARPD with canine implant in both schemes. The lowest stress in the implant(s) was recorded in IARPD with canine-second molar implants in GP and IARPD with canine-first molar implants in CG.</p><p><strong>Conclusion: </strong>Dental implants reduced the total displacement of IARPDs, increased stress in mechanical components, and did not affect stress distribution in biological components. Insertion of two implants decreased implant stress. The GP scheme caused greater stress on mechanical components.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":"e70078"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11746924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Evaluation of the Antibacterial Properties of an Experimental Herbal Gel Against Cariogenic Bacteria.","authors":"Marco Sánchez-Tito, Lidia Yileng Tay, Francisco Zea-Gamboa, Raúl Cartagena-Cutipa, Alysson Flores-Gómez, Bruno Spigno-Paco, Brando Raul Mendoza Salinas, Jose Elias Zuñiga Calcina, Ingrit Elida Collantes Díaz","doi":"10.1002/cre2.70076","DOIUrl":"10.1002/cre2.70076","url":null,"abstract":"<p><strong>Background: </strong>Recently, products with antibacterial properties derived from medicinal plants have increased as an alternative to conventional drugs. Thus, this study aimed to formulate and evaluate the antibacterial activity of an experimental gel based on Grindelia tarapacana essential oil in a bacterial consortium.</p><p><strong>Material and methods: </strong>The composition of the essential oil (EO) was determined using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity of the EO against Streptococcus mutans ATCC 25175, Streptococcus sanguinis ATCC 10556, and Streptococcus salivarius ATCC 13419 was evaluated using an Agar disc diffusion and minimum inhibitory concentration methods. Five formulations of the experimental gel were prepared at 0.25%, 0.5%, 1%, 1.5%, and 2% (v/v). The antibacterial susceptibility test was evaluated using an Agar-Well diffusion assay against a bacterial consortium of S. mutans, S. sanguinis, and S. salivarius. The physical properties, pH, spreadability, gel morphology, phase separation, and drug release were evaluated. The experimental gels were compared with a chlorhexidine gel. Data were analyzed with one-way ANOVA and Kruskal-Wallis tests with a significant level of 5%.</p><p><strong>Results: </strong>The major components of the EO were bornyl acetate, α-isomethyl-E-nerolidol, germacrene B, E-nerolidol, α-cedrene-epoxide, fokienol, and 10-epi-γ-eudesmol. All formulations were effective in inhibiting bacterial growth. The 2% concentration presented inhibition zones (18.14 ± 1.01 mm) similar to those observed for the chlorhexidine gel (p > 0.05). All formulations were stable, without signs of separation, with adequate physical properties, and no significant differences were observed regarding the drug content with the chlorhexidine gel (p > 0.05).</p><p><strong>Conclusions: </strong>The experimental gels based on G. tarapacana EO presented good physicochemical properties and were highly effective in inhibiting the growth of a cariogenic bacterial consortium.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":"e70076"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Correlation Between Langerhans Cells Population and Prognosis of Tongue Squamous Cell Carcinoma.","authors":"Narges Ghazi, Nasrollah Saghravanian, Pooya Saeedi, Mohammad Mahdi Maboudinezhad","doi":"10.1002/cre2.70080","DOIUrl":"https://doi.org/10.1002/cre2.70080","url":null,"abstract":"<p><strong>Background and objective: </strong>Tongue squamous cell carcinoma (TSCC) is the most prevalent oral cancer. Despite considerable advancements in treatment, the 5-year survival rate remains relatively unchanged. Langerhans cells (LCs) play an important role in antitumor immunity. Therefore, we attempt to evaluate the correlation between the LC count and disease prognosis.</p><p><strong>Materials and methods: </strong>Histopathologic slides from 24 cases, with at least 2 years of follow-up, were selected and categorized into early-stage (12 cases) and advanced-stage (12 cases) groups. An additional 12 slides of normal tissue comprised the control group. Immunohistochemical staining with the CD1a marker was performed to analyze the density of LCs. Statistical analysis assessed the impact of CD1a immune expression on patient survival and other variables such as age, gender, stage, and histopathological grade.</p><p><strong>Results: </strong>Comparison of CD1a+ cell counts across the three groups revealed a significant decrease in the advanced group. Furthermore, a lower count of CD1a+ cells correlated with poorer disease-free survival (DFS) (p < 0.001) and overall survival (OS) (p = 0.049). Although the CD1a+ cell count did not independently affect OS significantly (p = 0.210), it did show a significant impact on DFS as an independent variable (p = 0.002).</p><p><strong>Conclusion: </strong>The significant correlation between CD1a expression and patients' prognosis and survival rates suggests that CD1a+ cells could serve as a crucial prognostic factor in the management and treatment of TSCC.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":"e70080"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Self-Reported Oral Health and Dental Fear Among Illicit Drug Users in Northern Finland","authors":"Raija Vainionpää,&nbsp;Antti Tiisanoja,&nbsp;Outi Kokkola,&nbsp;Pirkko Riipinen,&nbsp;Vuokko Anttonen","doi":"10.1002/cre2.70055","DOIUrl":"10.1002/cre2.70055","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>About 10% of adults in northern Finland have severe dental fear, but there is a lack of detailed knowledge about dental fear among illicit drug users. The aim of the study was to evaluate the prevalence of dental fear and its association with self-reported oral health and health behavior as well as background factors of the customers of substance abuse services living in the region of Oulu, Northern Ostrobothnia, Finland.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>One hundred seventeen volunteers, either active or former illicit drug users, were interviewed face-to-face about their background factors, health and health behaviors, and use of illicit drugs. The Modified Dental Anxiety Scale (MDAS) was used to assess a situation-specific level of dental anxiety. For analyses, Pearson's <i>χ</i>² test, Fisher's exact test, ANOVA, and logistic regression analysis were performed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Three out of four participants had at least moderate dental fear (MDAS ≥ 10) and 24% had severe dental fear (MDAS ≥ 19), with the average MDAS being 14.0 (SD 5.7). Participants in drug rehabilitation reported significantly more dental fear than the rest of the participants. Severe dental fear was associated (adjusted) with previous painful experiences OR 10.8 (2.3–52.0) and poor behavior by dental personnel OR 4.1 (1.2–13.9).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Dental fear is common among illicit drug users, and it is, particularly, associated with previous painful experiences and poor behavior by dental personnel.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"10 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a Quantitative Method for the Extraction of Nicotine and Cotinine in Gingival Tissue and Relationship Between Gingival Intoxication With Conventional Smoking Biomarkers: A Pilot Study","authors":"Leila Salhi,&nbsp;Samuel Hazout,&nbsp;Dorien Van hede,&nbsp;France Lambert,&nbsp;Corinne Charlier,&nbsp;Marine Deville","doi":"10.1002/cre2.70022","DOIUrl":"10.1002/cre2.70022","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Smoking is considered a major risk factor for periodontitis genesis and progression. In clinical studies, specific indicators have been used to characterize the smoking status of the patient as the number of cigarettes consumed (NCC), the pack-years (PY), or Fagerström Test for Nicotine Dependence (FTND). However, available literature is missing on the relationship between cotinine gingival intoxication and smoking indicators. First, the development of a quantitative method for the extraction of nicotine and cotinine in gingival tissue. Second, to investigate the relationship between gingival intoxication and conventional smoking biomarkers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Fourteen smoker patients were included in the study. After clinical data collection, salivary and gingival samples collection, toxicological analyses were performed using liquid extraction after enzymatic digestion (subtilisin) and ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS/MS).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Gingival cotinine quantification was successfully performed in 14 samples (100%) with a mean of 0.280 ng/mg (range = 0.094–0.505). Only FTND was statistically associated with gingival cotinine levels (<i>p</i> = 0.0072; <i>r</i>² = 0.60). Gingival nicotine quantification was achieved in 12 of the 14 gingival samples (86%) with a mean of 0.384 ± 1.00 ng/mg (range = 0.03–3.84). Gingival nicotine was statistically associated with NCC (<i>p</i> = 0.032; <i>r</i>² = 0.55), PY (<i>p</i> = 0.0011; <i>r</i>² = 0.76), and FTND (<i>p</i> = 0.016; <i>r</i>² = 0.60). Salivary nicotine and cotinine levels were statistically associated with, respectively, NCC (<i>p</i> = 0.030; <i>r</i>² = 0.34), and NCC (<i>p</i> = 0.0094; <i>r</i>² = 0.63) + PY (<i>p</i> = 0.0078; <i>r</i>² = 0.64).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This pilot study established a quantitative extraction method for nicotine and cotinine from human gingival samples. Additionally, FTND was associated with gingival cotinine. However, further large-scale studies are needed to confirm the relationship between nicotine dependence and gingival intoxication.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"10 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Implant Position Accuracy in Guided Implantology: The Role of Drill Play Under Lateral Forces","authors":"Vasilios Alevizakos,&nbsp;Yannick Stryga,&nbsp;Constantin von See","doi":"10.1002/cre2.70056","DOIUrl":"10.1002/cre2.70056","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study investigates the impact of drill play on the precision of implant positioning under lateral forces in guided implantology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>An in vitro experiment was conducted using artificial bone blocks and the SIC Invent-guided surgery implant system. Custom drill guides were designed, and 3D-printed, using three sleeve types: sleeveless, a big sleeve, and a small sleeve. Drillings were performed with varying lateral forces, and deviations in angle, depth, and position were measured.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Sleeveless guides showed the highest variability in deviations, with a maximum deviation of 3.92 mm under extreme lateral forces. Big sleeve guides provided the most consistent precision, with deviations ranging from 0.42 to 1.33 mm. Small sleeve guides showed moderate precision, with deviations from 0.14 to 2.17 mm. Higher lateral forces generally increased deviations across all guide types.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Drill play significantly affects the precision of guided implant drilling, with lateral forces causing deviations from the planned implant position. Big sleeve guides offer better precision under lateral loads compared to sleeveless and small sleeve guides. Strict adherence to the drilling protocol is essential to minimize errors and ensure optimal implant positioning.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"10 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skeletal and Dental Effects of Forsus Fatigue Resistance Device Versus Twin Block Appliance for Class II Malocclusion Treatment in Growing Patients: A Systematic Review","authors":"Bahaa Aldeen Jeha,&nbsp;Rania Haddad","doi":"10.1002/cre2.70054","DOIUrl":"10.1002/cre2.70054","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study systematically searched the literature and assessed the available evidence to compare the efficacy of Forsus Fatigue Resistance Device (FRD) versus Twin Block Appliance (TBA) in treating class II malocclusion.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The search for published literature was published up to May 28, 2024. The databases were included in the search: MEDLINE, PubMed, EMBASE, Cochrane Oral Trials Register, Tripe, Web of Science, and Scopus. Additionally, unpublished literature was searched on ClinicalTrials.gov, the National Research Register, and ProQuest Dissertations and Theses. All eligible studies were carefully reviewed and two reviewers independently extracted data. In cases of disagreement, an arbiter was consulted for resolution.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Two randomized controlled trials (RCTs) and five non-RCTs were included in this review. The total number of patients included in the studies examining SNA, SNB, and ANB was 254. The studies also looked at the variables Go-Gn, L1-ML, and U1-SN, with 279, 205, and 277 patients included for each variable, respectively. According to the evidence reported, TBA showed greater skeletal effects in terms of mandibular length and advancement. The pooled estimate revealed a statistically significant 1.3° increase in the SNB, and a decrease of −1.34° in the ANB angles for patients treated with TBA compared with those treated with FRD, with no statistically significant differences in the SNA angle. Most studies had a moderate risk of bias, while only two studies had a high risk of bias.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>FRD has been proven to be an effective treatment device for correcting ANB and restricting SNA angle, similar to TBA. However, TBA appears to offer better mandibular length and SNB outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"10 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9","authors":"Marzieh Danaei,&nbsp;Milad Mollaali,&nbsp;Vida Fakharmohialdini,&nbsp;Hamidreza Poureslami,&nbsp;Fatemeh Sadat Sajadi,&nbsp;Elham Farokh Gisour,&nbsp;Fatemeh Jahanimoghadam,&nbsp;Aida Gholampour,&nbsp;Mehrnaz Foroudisefat,&nbsp;Arezoo Mirshekari,&nbsp;Raziyeh Shojaeipour","doi":"10.1002/cre2.70039","DOIUrl":"10.1002/cre2.70039","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Lactobacilli and <i>Streptococcus mutans</i> are stigmatized as cariogenic bacteria, but few studies have simultaneously examined the quantitative and qualitative aspects of lactobacilli and <i>S. mutans</i> in childhood dental caries. Therefore, this study aimed to detect the presence of <i>S. mutans</i> and lactobacilli in dental caries using Snyder's test, colony counting, and PCR in the primary teeth of Iranian children with dental caries.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A cross-sectional study was conducted in Kerman, Iran, from March to Jun 2024. After dental examinations, 120 eligible children aged 5–9 were chosen using simple random sampling and classified into four groups based on their decayed, missing, and filled (primary) teeth (dmft) index: Group 1 (dmft = 0), Group 2 (dmft = 4–6), Group 3 (dmft = 7–9), and Group 4 (dmft = 10–13). The salivary levels of lactobacilli and <i>S. mutans</i> were calculated using colony counting (CFU/ml). Moreover, Snyder's test was applied to evaluate caries activity. PCR was also performed for molecular detection of lactobacilli (<i>16S rRNA</i> gene) and <i>S. mutans (gftB</i> gene). Lastly, the association between bacterial counting, molecular findings, and Snyder's test was estimated through statistical methods using SPSS 27.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant differences were found between the age and the PCR results of lactobacilli and <i>S. mutans</i> among all dmft Groups (<i>p</i> &lt; 0.05). Moreover, positive significant correlations were observed between the counts of lactobacilli and <i>S. mutans</i> in dmft Group 1 compared to other dmft Groups (<i>p</i> &lt; 0.05). Furthermore, the counts of these two bacteria differed significantly in Snyder's test (<i>p</i> &lt; 0.05). However, Snyder's test differed significantly from the <i>S. mutans</i> PCR (<i>p</i> &lt; 0.05), but not from the lactobacilli PCR (<i>p</i> &gt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The results of the study could potentially be considered a promising and cost-effective screening program to identify children who are susceptible to dental caries.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"10 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}