Clinical and Experimental Dental Research最新文献

{"title":"Diagnostic Accuracy of Salivary Thiols Point-of-Care Test for Detecting Subgingival Dysbiosis in Adults: A Cross-Sectional Study","authors":"Ahmed Khocht,&nbsp;Thomas E. Rams,&nbsp;Tsute Chen,&nbsp;Jon B. Suzuki","doi":"10.1002/cre2.70485","DOIUrl":"10.1002/cre2.70485","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Many bacteria implicated in the pathogenesis of periodontitis produce thiol compounds containing sulfhydryl (-SH) groups. This cross-sectional study used a prototype chromogenic test strip to investigate the relationship between whole saliva -SH levels and subgingival bacterial species in subjects with and without periodontal disease.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Ninety-six subjects were enrolled. Full-mouth comprehensive clinical periodontal evaluations were performed. Subjects were divided into 3 groups: periodontally healthy, gingivitis, and periodontitis. Subgingival plaque samples were collected and analyzed for their content of 40 bacterial species using checkerboard DNA–DNA hybridization. Subgingival dysbiosis severity was determined with a modified Subgingival Microbial Dysbiosis Index (mSMDI). Salivary thiol levels were determined semi-quantitatively with a chromogenic strip dipped in pooled saliva on the tongue surface.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Low and high strip scores were uniquely associated with contrasting groups of subgingival bacteria. Individuals with high strip scores harbored increased levels of <i>P. gingivalis, P. intermedia, T. forsythia, P. nigrescens, E. nodatum, and T. denticola</i> as well as higher mSMDI scores than individuals with low strip scores. Furthermore, strip scores showed high specificity (93%) in correctly identifying individuals with normobiotic subgingival microbiota.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Low salivary thiol levels are useful indicators of subgingival normobiosis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fractal Analysis of Jaw Bone Structure in Individuals With Dental Fluorosis: A Retrospective Cross-Sectional Study","authors":"Ozlem Gormez,&nbsp;Hasan Huseyin Yilmaz,&nbsp;Abdulkadir Cakir","doi":"10.1002/cre2.70480","DOIUrl":"10.1002/cre2.70480","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This retrospective cross-sectional study aimed to evaluate differences in maxillary and mandibular trabecular bone structure in individuals with dental fluorosis using fractal dimension (FD) analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>The study included 47 individuals: 24 with dental fluorosis and 23 controls. Fluorosis severity was assessed using the Thylstrup–Fejerskov Index and classified as moderate (<i>n</i> = 13) or severe (<i>n</i> = 11). FD analysis was performed on standardized regions of interest on digital panoramic and digitized analog periapical radiographs using ImageJ. Normality was assessed using the Shapiro–Wilk test. Region-specific FD values were compared using independent-samples <i>t</i>-tests or Mann–Whitney <i>U</i> tests, as appropriate. Generalized estimating equation (GEE) analyses were used to account for within-participant correlation among repeated regional measurements.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In region-specific comparisons, FD was significantly lower in the fluorosis group than in controls in the 33–34 panoramic region (1.46 ± 0.04 vs. 1.48 ± 0.03; <i>p</i> = 0.048). FD was significantly higher in severe than moderate fluorosis in the 13–14 panoramic region (1.36 ± 0.05 vs. 1.28 ± 0.06; <i>p</i> = 0.004) and the 11–12 periapical region (1.63 ± 0.01 vs. 1.61 ± 0.02; <i>p</i> = 0.019). GEE analysis showed a significant average marginal fluorosis-control difference for panoramic radiographs (mean difference = −0.0210, 95% CI: −0.0386 to −0.0034; <i>p</i> = 0.020), without a significant group-by-region interaction (<i>p</i> = 0.840). For fluorosis severity, the severity-by-region interaction was significant for panoramic radiographs (<i>p</i> = 0.004), while average marginal periapical FD was higher in the severe subgroup (<i>p</i> = 0.028).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>FD analysis identified differences in radiographic trabecular pattern complexity associated with dental fluorosis, although findings varied by anatomical region and imaging modality. Given the small sample, multiple regional comparisons, and moderate reproducibility of periapical measurements, these findings should be considered exploratory and require confirmation in larger studies using standardized imaging protocols.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Properties, Color Stability, and Bacterial Adhesion of Implant-Supported Provisional Restorative Materials: In Vitro Study","authors":"Júlia Bárbara Medeiros Galvão,&nbsp;Richelle Nunes Costa,&nbsp;Aliane da Silva Bezerra,&nbsp;Liliane Cristina Nogueira Marinho,&nbsp;Ana Luísa de Barros Pascoal,&nbsp;Pedro Henrique Sette de Souza,&nbsp;André Ulisses Dantas Batista,&nbsp;Patrícia dos Santos Calderon","doi":"10.1002/cre2.70461","DOIUrl":"10.1002/cre2.70461","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To evaluate surface roughness, wettability, color stability, and bacterial adhesion of different materials used in implant-supported provisional restorations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Eighty samples (6 × 6 × 2 mm ± 0.2 mm) were fabricated and distributed into four groups (<i>n</i> = 20): Bis-acryl composite resin (BCR); CAD-CAM PMMA resin (Milled); Auto-polymerized acrylic resin (APR); prefabricated acrylic tooth (PT). Surface roughness was measured using a 3D optical profilometer, wettability by the sessile drop method, and color stability with a spectrophotometer after immersion in distilled water and coffee. Bacterial adhesion was assessed through <i>Streptococcus mutans</i> biofilm formation and quantified spectrophotometrically after crystal violet staining. Data were analyzed using Kruskal–Wallis and one-way ANOVA with Tukey's post hoc test (<i>α</i> = 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Bis-acryl composite resin (BCR) showed the lowest surface roughness, while prefabricated acrylic tooth (PT) exhibited higher roughness and contact angle values (<i>p</i> &lt; 0.001) and the greatest color change (Δ<i>E</i> ≥ 3.3). BCR demonstrated the highest bacterial adhesion, whereas CAD-CAM PMMA resin (Milled) showed the lowest adhesion.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The behavior of provisional restorative materials is influenced by their composition, affecting surface, optical, and biological performance. Lower surface roughness did not result in reduced biofilm formation. CAD/CAM-milled and Auto-polymerized resins showed more favorable overall performance.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Clinical and Histopathological Profiles of Oral Potentially Malignant Disorders With Oral Epithelial Dysplasia: Focus on Subepithelial Lymphoplasmacytic Infiltrate and Its Grading","authors":"Abbas Saleem Khan,&nbsp;Shahzad Ahmad,&nbsp;Hina Shabir,&nbsp;Shazia Iqbal,&nbsp;Tehmina Naushin,&nbsp;Muhammad Nadeem Baig,&nbsp;Aneela Bashir,&nbsp;Maria Hanif,&nbsp;Mohammed Saad Alqarni,&nbsp;Rakhi Issrani","doi":"10.1002/cre2.70482","DOIUrl":"10.1002/cre2.70482","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study was conducted to describe the clinical and histopathological features of OPMDs characterized by OED with a special focus on the subepithelial LPI and its grades.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>This cross-sectional study was conducted on hematoxylin and eosin-stained slides of 82 diagnosed cases of OPMDs with OED. Histologic review was performed based on the WHO and binary grading system for OED. Subepithelial LPI and its grades were also noted and reported. <i>χ</i>² test and Fisher's exact test were used to compare different categorical variables related to OPMDs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean age of OPMD cases with OED was 58.51 ± 12.38, with a male-to-female ratio of 2.28:1. OED lesions were mostly (<i>N</i> = 41; 50%) seen on the buccal mucosa, retromolar area, and vestibule of the oral cavity. Oral leukoplakia was the most (<i>N</i> = 52; 63.4%) commonly diagnosed clinical variant of OPMDs. Drop-shaped rete ridges (<i>N</i> = 78; 95.12%) and hyperchromasia (<i>N</i> = 76; 92.68%) were the most commonly occurring architectural and cellular alterations of OED, respectively. The overall interobserver agreement for various histopathologic variables ranged from moderate to perfect. Histologically, moderate dysplasia was the most frequently occurring grade (<i>N</i> = 37; 45.12%). Most (<i>N</i> = 45; 54.9%) of the OPMDs were marked as high-risk dysplasia. Subepithelial LPI was significantly (<i>p</i> &lt; 0.01) related to the histologic grades of OED lesions. The grades of LPI were significantly (<i>p</i> = 0.018) related to the binary grading system only.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The subgrades of LPI were significantly associated with the binary grading system of OED, suggesting that subepithelial LPI may reflect underlying microenvironmental changes related to dysplasia severity.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13654314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Clinical Effects of Essential Oil–Containing Mouthwash and Chlorhexidine Mouth Wash in Patients Undergoing Fixed Orthodontic Treatment – Three-Month Follow-up Randomized-Controlled Trial","authors":"Fazli Rabi,&nbsp;Naveed Sadiq,&nbsp;Muhammad Jamil,&nbsp;Sadia Asif Samdani,&nbsp;Waqas Naseem,&nbsp;Ferdous Bukhary,&nbsp;Umar Hussain,&nbsp;Alessandra Campobasso,&nbsp;Nikolaos Pandis","doi":"10.1002/cre2.70477","DOIUrl":"10.1002/cre2.70477","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to compare the clinical effects of chlorhexidine (CHX) and essential oil–containing mouthwash (EOM) on periodontal health in patients undergoing fixed orthodontic treatment over a 3-month follow-up period.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>This double-blinded, parallel-arm randomized-controlled clinical trial with a 1:1 allocation ratio was conducted at the Department of Orthodontics, Saidu College of Dentistry, Swat, Pakistan. Sixty participants aged 13–40 years undergoing fixed orthodontic treatment were randomly allocated to receive either 0.12% chlorhexidine mouthwash or Listerine essential oil mouthwash, dispensed in identical coded containers to ensure blinding. Participants were instructed to rinse with 10 mL of the assigned mouthwash twice daily for 30 s for 3 months while maintaining routine oral hygiene practices. The primary outcomes were the plaque index and the gingival index. Tooth staining was assessed as a secondary outcome (harm). Clinical outcomes, including the plaque index, the gingival index, and the stain index, were recorded at baseline, 1, 2, and 3 months by a calibrated examiner. Data were analyzed using generalized estimating equation models to evaluate longitudinal changes between groups, with statistical significance set at <i>p</i> ≤ 0.05.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The gingival index showed a significant change over time (Wald <i>p</i> = 0.02), with no statistically significant overall difference between the CHX and Listerine groups (Wald <i>p</i> = 0.07). The plaque index was significantly higher in the CHX group than in the Listerine group (Wald <i>p</i> &lt; 0.001). The stain index was also significantly higher in the CHX group than in the Listerine group (Wald <i>p</i> &lt; 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Both mouthwashes were associated with changes in periodontal health during fixed orthodontic treatment. Compared with CHX, EOM was associated with significantly lower plaque index and stain index scores, whereas no statistically significant overall difference between groups was observed for the gingival index.</p>\u0000 \u0000 <p><b>Trial Registration:</b> This trial was retrospectively registered at ClinicalTrials.gov (Identifier: NCT07119840).</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13640973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dentofacial Characteristics in Patients With Epidermolysis Bullosa—A Cross-Sectional Study","authors":"Sebastián Véliz,&nbsp;Susanne Krämer,&nbsp;Colomba Besa-Witto,&nbsp;Pedro Diz-Dios","doi":"10.1002/cre2.70478","DOIUrl":"10.1002/cre2.70478","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Objectives&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Epidermolysis bullosa (EB) is a rare genetic disease characterized by skin and mucosal fragility caused by pathogenic variants in structural protein genes. Malocclusions have been previously described in patients with EB, mainly in case reports, with no cohort studies characterizing dentofacial characteristics across all EB types. This study aims to describe and compare the dentofacial characteristics of patients with EB across all types in a nationally representative cohort.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Material and Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;A cross-sectional study examined 101 patients with genetically confirmed EB diagnosis at a national reference center (2023 and 2024). Occlusal and extraoral characteristics were assessed by calibrated examiners (Cohen's Kappa: Intrarater 0.925, Interrater 0.931), including overjet, Angle's molar class, overbite, midline deviation, posterior transverse relationship, dental anomalies, crowding, facial profile, type, and symmetry. Associations were examined using Fisher's exact test with Cramér's &lt;i&gt;V&lt;/i&gt; as effect size measure, and Kruskal–Wallis test with epsilon-squared; &lt;i&gt;p&lt;/i&gt; values were adjusted using the Benjamini–Hochberg false discovery rate procedure.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The sample included: EB simplex (EBS, &lt;i&gt;n&lt;/i&gt; = 26), junctional EB (JEB, &lt;i&gt;n&lt;/i&gt; = 6), dominant dystrophic EB (&lt;i&gt;n&lt;/i&gt; = 18), recessive dystrophic EB (RDEB, &lt;i&gt;n&lt;/i&gt; = 49), and kindler EB (&lt;i&gt;n&lt;/i&gt; = 2). RDEB presented the highest prevalence of posterior transverse anomalies (74.5%, 95% CI 60.5–84.7; Cramér's &lt;i&gt;V&lt;/i&gt; = 0.504, &lt;i&gt;p&lt;/i&gt;[FDR] &lt; 0.001) and altered overbite (Kruskal–Wallis &lt;i&gt;p&lt;/i&gt; = 0.022, &lt;i&gt;ε&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt; = 0.106), with a significant difference compared to EBS (post-hoc adjusted &lt;i&gt;p&lt;/i&gt; = 0.022). All participants with JEB presented dental anomalies (100%, 95% CI 61.0–100.0; Cramér's &lt;i&gt;V&lt;/i&gt; = 0.762, &lt;i&gt;p&lt;/i&gt;[FDR] &lt; 0.001). 53.1% of RDEB participants presented three or more dentofacial features coherent with growth restriction.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusions&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Dentofacial anomalies vary according to the EB type. RDEB showed the highest prevalence of posterior transverse anomalies and altered overbite, and JEB presented universal dental anomalies. These findings support prioritizing and tailoring orthodontic screening by EB type. Future studies should address genotype–phenotype correlations, multicentre designs, and the relationship between dentofacial anomalies and orofacial functional impairments.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 ","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13640968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hantavirus in Dentistry: Occupational Exposure Considerations and a Proposed Tiered Infection Prevention and Control Framework—A Narrative Review","authors":"Parsa Vafaei,&nbsp;Morteza Banakar,&nbsp;Mostafa Alam,&nbsp;Mohammad Javad Askari,&nbsp;Mohsen Golkar","doi":"10.1002/cre2.70481","DOIUrl":"10.1002/cre2.70481","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The aim of this study is to synthesize evidence on hantavirus transmission and occupational exposure pathways relevant to dentistry, and to propose an evidence-informed tiered infection prevention and control (IPC) framework that distinguishes established from theoretical risk.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The initial narrative search covered database inception through May 15, 2026 in MEDLINE/PubMed, Embase, Web of Science, Scopus, Google Scholar, and WHO/CDC/ECDC/OSHA sources, followed by a targeted PubMed/MEDLINE and public-health guidance update through August 18, 2026. Approximately 1450 records were identified. After duplicate removal, 1070 records were subjected to title/abstract screening, 215 full-text records were assessed for eligibility, and 45 sources were included in the final synthesis, comprising 34 peer-reviewed publications and 11 gray-literature or public-health guidance sources.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Environmental exposure to infected-rodent contamination is the established occupational pathway. Limited person-to-person transmission is documented for the Andes virus (ANDV), but no dental transmission has been reported. ANDV RNA and culture-based infectivity have been demonstrated in some oral/respiratory fluids; however, dental-aerosol transmission has not been shown. Dental-specific occupational risk therefore remains unquantified.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>No dental transmission has been documented, and patient-related risk remains uncertain. Standard precautions and rodent control are foundational; suspected or confirmed ANDV warrants transmission-based precautions and airborne precautions for aerosol-generating procedures according to current guidance. The proposed dental tiers are evidence-informed, author-derived, and unvalidated.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13640971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral Seal Composite Barrier (PSCB) for Management of Palatal Donor Sites in Mucogingival Surgery: A Randomized Clinical Trial","authors":"Moein Khojaste,&nbsp;Farid Shiezadeh,&nbsp;Zahra Moslehitabar","doi":"10.1002/cre2.70470","DOIUrl":"10.1002/cre2.70470","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Objectives&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Management of the palatal donor site following periodontal plastic surgery remains challenging because of postoperative pain, bleeding, and patient discomfort. This study aimed to evaluate the efficacy of a chairside-fabricated composite barrier in reducing morbidity at the palatal donor site.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Materials and Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;In this randomized, parallel-group clinical trial, 50 patients undergoing soft tissue augmentation were allocated to either a test group (composite barrier + collagen sponge) or a control group (collagen sponge alone). Intraoperative measurements included palatal tissue thickness, graft dimensions, and donor site management time. Postoperative outcomes (pain, bleeding, discomfort, chewing difficulty, stress, analgesic consumption, and willingness for retreatment) were assessed at prespecified time points during the first 2 postoperative weeks. Donor sites complications were specifically assessed on postoperative day 7.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;All participants completed the study without dropouts. Compared with controls, the composite barrier group required significantly shorter wound management time (210.42 ± 52.04 vs. 445.76 ± 77.43 s; &lt;i&gt;p&lt;/i&gt; &lt; 0.001) and lower analgesic consumption during the first week (1328.00 ± 846.33 vs. 4160.00 ± 1366.26 mg; &lt;i&gt;p&lt;/i&gt; &lt; 0.001). Pain scores were significantly lower in the composite barrier group on postoperative days 1–7 (&lt;i&gt;p&lt;/i&gt; &lt; 0.001), slightly higher on day 10 (&lt;i&gt;p&lt;/i&gt; = 0.007), and similar on day 14. Bleeding scores were lower during the first 4 postoperative days (&lt;i&gt;p&lt;/i&gt; &lt; 0.001). Patient-reported discomfort, chewing difficulty, and stress were significantly reduced in the composite barrier group (&lt;i&gt;p&lt;/i&gt; &lt; 0.001), which also showed greater willingness for retreatment (7.80 ± 1.15 vs. 2.04 ± 1.34; &lt;i&gt;p&lt;/i&gt; &lt; 0.001). No necrosis, infection, or hematoma occurred.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusions&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Application of a composite barrier for palatal donor site management reduces the time required to achieve intraoperative hemostasis and is associated with lower postoperative pain, bleeding, and patient discomfort. This approach may improve patient-reported outcomes following periodontal plastic surgery.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Trial Registration&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;This project was registered in the Iranian Registry of Clinical Trials under the ID (IRCT20250513065721N2","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13640974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical Anesthetics Effectiveness in Management of Orthodontic Pain: A Systematic Review of Randomized Controlled Trials","authors":"Qasim Khalid,&nbsp;Abbas Naseem,&nbsp;Waqas Naseem,&nbsp;Adnan Jehangir,&nbsp;Umar Hussain,&nbsp;Maryam Altuhafy,&nbsp;Junad Khan","doi":"10.1002/cre2.70473","DOIUrl":"10.1002/cre2.70473","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Orthodontic treatment can be associated with pain, particularly during separator placement, appliance insertion, or activation. Topical anesthetics may offer a simple, locally applied alternative to systemic analgesics, but their effectiveness remains uncertain. The objective of this review was to evaluate the efficacy of topical anesthetics, compared to other treatments or no treatment, in reducing orthodontic pain.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Eligible studies were randomized controlled trials (RCTs) comparing topical anesthetics (gels or patches) with placebo, no treatment, or other active pain-control interventions in orthodontic patients. Five databases (PubMed, Scopus, Cochrane CENTRAL, Embase, Web of Science) were searched up to 14th March 2026, with no language or date restrictions. Data were extracted in duplicate, and the risk of bias was assessed using the ROB-2 tool. Due to heterogeneity, only a qualitative synthesis was performed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Nine RCTs (456 participants, mean age 14–35 years; 36.3% male) conducted in four countries were included. Benzocaine was the most investigated, followed by lidocaine, prilocaine/lidocaine, and menthol. Most studies examined separator-induced pain. Eight of nine trials reported significant reductions in pain intensity with topical anesthetics at one or more time points, particularly within the first 24 to 72 h. Benzocaine consistently showed superior outcomes compared with placebo. Lidocaine and prilocaine/lidocaine provided rapid analgesia, especially in patients with high anxiety or low pain thresholds. Menthol patches also showed significant short-term pain reduction in two studies. Risk of bias was moderate in most trials.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Topical anesthetics can be effective in reducing short-term orthodontic pain. Their use may reduce reliance on systemic analgesics and improve patient comfort during the initial stages of treatment. However, evidence quality remains limited, and further high-quality RCTs with standardized protocols are needed.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13640979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Site-Specific Associations Between CBCT-Derived Gray Value and Fractal Dimension and DEXA T-Scores in Postmenopausal Women: A Cross-Sectional Analytical Study","authors":"Golnoush Saeidi,&nbsp;Mohammad Mahdi Maleki,&nbsp;Abbas Shokri,&nbsp;Leili Tapak","doi":"10.1002/cre2.70474","DOIUrl":"10.1002/cre2.70474","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Osteoporosis is a silent systemic skeletal disease characterized by microarchitectural deterioration and increased fracture risk; thus, early case-finding is essential. Although dual-energy X-ray absorptiometry (DEXA) is the reference standard for bone mineral density (BMD) assessment, limited accessibility and cost restrict its use for large-scale screening. Given that cone-beam computed tomography (CBCT) is routinely obtained in dental practice, image-derived parameters—gray value (GV) and fractal dimension (FD)—may provide insight into their association with systemic bone mineral density measured by DEXA in postmenopausal women, without additional radiation exposure.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This cross-sectional study included 63 postmenopausal women aged 50–70 years undergoing dental CBCT (NewTom 3G; standardized acquisition). Six trabecular regions of interest (ROIs) were evaluated: mandibular retromolar, mandibular premolar, mandibular anterior (central), maxillary premolar, maxillary anterior (central), and maxillary tuberosity. GV values were extracted using OnDemand3D-Dental from standardized 25 × 25-pixel ROIs positioned to exclude cortical bone and dental structures. FD was calculated in ImageJ using a box-counting method following Gaussian blur subtraction, binarization, and skeletonization. Lumbar spine and hip BMD were measured by DEXA and categorized according to WHO criteria. Statistical analyses included <i>t</i>-tests/ANOVA, Pearson correlation (<i>α</i> = 0.05). Intra-observer reliability was assessed with intraclass correlation coefficients (ICC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>DEXA classified 49.2% as normal, 34.9% as osteopenic, and 15.9% as osteoporotic. Lumbar assessment identified more non-normal cases than the hip evaluation. Reliability was good (ICC &gt; 0.70). Although mean GV and FD did not differ significantly between groups, site-specific GV–FD correlations were observed. Notably, FD at the maxillary premolar correlated with lumbar T-scores in the non-normal group (<i>p</i> = 0.030).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>While GV and FD did not discriminate BMD categories overall, region-dependent associations between CBCT-derived parameters and systemic BMD were observed, suggesting potential clinical relevance in dental imaging. These exploratory findings should not be interpreted as diagnostic or screening indicators and require confirmation in larger studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13634604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}