Clinical and Experimental Dental Research最新文献

{"title":"Impact of Thermal Treatment on Growth Factor Levels in Injectable Horizontal Platelet-Rich Fibrin: An In Vitro Study.","authors":"Deshetti Ketan, Anita Kulloli, Sharath Shetty, Santosh Martande, Shivani Yerte, Avinash Sanap, Shubham Lawate, Chaitaly Munot, Dileep Sharma","doi":"10.1002/cre2.70353","DOIUrl":"10.1002/cre2.70353","url":null,"abstract":"<p><strong>Background: </strong>Platelet-rich fibrin (PRF) is widely recognized for its regenerative properties in periodontal therapy, particularly through the release of endogenous growth factors. Injectable horizontal PRF (H-PRF), a recent advancement, has gained interest due to its improved cell distribution and structural integrity. The aim of this study was to analyze and compare the levels of growth factors released from injectable H-PRF subjected to different thermal treatments.</p><p><strong>Methods: </strong>Venous blood samples from 16 healthy participants (aged 18-25 years) were collected and divided into four groups: Group I (unheated control), Group II (37°C), Group III (45°C), and Group IV (60°C). Liquid H-PRF was prepared using horizontal centrifugation (700 RCF, 8 min), followed by controlled heating. Growth factor quantification was performed using the LEGENDplex Human Growth Factor Panel. Solidification and degradation profiles were also recorded. Statistical analyses included ANOVA and Bonferroni post hoc tests (significance at p < 0.05).</p><p><strong>Results: </strong>The solidification time decreased significantly with increasing temperature, ranging from 24 min in Group I to 5 min in Group IV. After 24 days, heated H-PRF gels (especially 45°C and 60°C) retained greater mass, indicating slower degradation. Significant intergroup differences were observed for angiopoietin-2 (p < 0.001), PDGF-AA (p = 0.002), TGF-α (p = 0.036), and VEGF (p = 0.011), with Group II showing the highest levels of angiogenic factors. No significant differences were noted for EGF, FGF-basic, HGF, and PDGF-BB.</p><p><strong>Conclusion: </strong>Thermal treatment of H-PRF at moderate temperatures (particularly 37°C and 45°C) enhances the release of angiogenic growth factors while improving the mechanical stability of the gel. However, the heating protocol will need to be customized based on the intended clinical applications.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70353"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13060760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of OMICS Technologies in Our Understanding of the Pathogenesis of Peri-Implantitis.","authors":"Farah Asa'ad, Akira Hasuike, Koki Yoshida, Ronaldo Lira-Junior, Akhilanand Chaurasia, Paula Milena Giraldo-Osorno, Carlos Garaicoa-Pazmino","doi":"10.1002/cre2.70350","DOIUrl":"10.1002/cre2.70350","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the contribution of OMICS technologies to the understanding of peri-implantitis pathogenesis from a host immune perspective.</p><p><strong>Materials and methods: </strong>A narrative review was conducted based on electronic searches of PubMed, MEDLINE, and Google Scholar up to October 2025, complemented by manual screening of reference lists. Search terms combined \"peri-implantitis\" with OMICS-related keywords, including genomics, epigenomics, transcriptomics, proteomics, metabolomics, RNA sequencing, single-cell and spatial transcriptomics, multi-omics, and machine learning. Studies were selected based on clinical relevance and their contribution to understanding peri-implantitis pathogenesis from a host immune perspective.</p><p><strong>Results: </strong>Among the studies included, most focused on transcriptomic analyses, while fewer investigated genomics, epigenomics, proteomics, or metabolomics. Integration across OMICS layers highlights peri-implantitis as a multilayered host-microbiome molecular ecosystem. Genomic variants affecting metal ion binding, cytoskeletal organization, and cell adhesion may predispose tissues to heightened immune sensitivity. Epigenomic analyses revealed differential DNA methylation of immune-regulatory and signaling genes, linking environmental exposures, such as smoking, to altered host responses. Transcriptomic studies, including bulk, single-cell, and spatial approaches, demonstrated dysregulated immune signaling, pro-inflammatory fibroblast-neutrophil interactions, oxidative stress, and dysregulated tissue remodeling. Proteomic profiling of peri-implant crevicular fluid confirmed elevated neutrophil-derived antimicrobial proteins and inflammatory mediators, reflecting active host defense responses. Metabolomic studies identified disease-specific alterations in amino acids, organic acids, and polyamines, which correlate with pathogenic microbial taxa and modulate immune and tissue responses. Collectively, these findings reveal convergent pathways of immune dysregulation, extracellular matrix disruption, tissue remodeling, and host-microbiome crosstalk as central features of peri-implantitis.</p><p><strong>Conclusions: </strong>OMICS analyses show that peri-implantitis is a complex host-microbiome molecular ecosystem. Integrated molecular insights provide a foundation for biomarker development, predictive diagnostics, and targeted interventions. However, future studies with larger cohorts and functional validation are needed to support clinical translation.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70350"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13075810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing Traditional Versus AI-Assisted TMJ Disorder Management Approaches: A Systematic Review and Meta-Analysis.","authors":"Vini Mehta, Annie Vathani, Praveen Kumar Gonuguntla Kamma, Saurabh Chaturvedi, Ankita Mathur, Toufiq Noor","doi":"10.1002/cre2.70370","DOIUrl":"https://doi.org/10.1002/cre2.70370","url":null,"abstract":"<p><strong>Objective: </strong>This systematic review and meta-analysis compared traditional diagnostic approaches with artificial intelligence (AI)-based techniques for temporomandibular joint disorders (TMDs) and evaluated their diagnostic accuracy.</p><p><strong>Methods: </strong>Literature searches across PubMed-MEDLINE, Scopus, Embase, Cochrane, and Google Scholar identified studies published between 1st January 2010 to 20th April 2025. Eligible studies used AI-based algorithms, such as deep learning (DL), machine learning (ML), or computer-aided diagnostic tools, for TMD diagnosis or management, reporting performance metrics including sensitivity, specificity, and accuracy. Traditional approaches, including clinical examinations, radiographic assessments, and standardized diagnostic criteria (DC/TMD), serve as comparators. Risk of bias was assessed using Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2). Meta-analysis was conducted in the form of pooled sensitivity and specificity.</p><p><strong>Results: </strong>Fourteen studies were included, comprising AI models trained on clinical and imaging data including cone beam computed tomography (CBCT), magnetic resonance imaging (MRI), and orthopantomogram (OPG). AI methods showed moderate-to-high diagnostic accuracy, with sensitivity ranging from 0.66 to 0.88 and specificity from 0.72 to 0.86. A meta-analysis of five studies showed pooled sensitivity and specificity estimates within these ranges. Among the included studies, AI models integrated radiomic as well as semantic features to achieve sensitivity from 0.82 to 0.93, and specificity from 0.76 to 0.90; however, evidence showed low certainty because bias risk was high (7/9 studies), sample sizes were small (mean n = 42), and external validation was absent in 8 of 9 studies.</p><p><strong>Conclusion: </strong>AI-assisted techniques offer significant potential to complement traditional TMD diagnostic methods by enhancing the diagnostic precision. However, owing to methodological limitations, further high-quality prospective studies with standardized reporting are needed to validate the use of AI in TMD management.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70370"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147764085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Microtensile Bond Strength of a 3D-Printed Hybrid Material Under Various Adhesive Strategies.","authors":"Patricia Freire-Nieto, Lissethe Peñate-González, Luis Jané-Chimeno, Anaïs Ramírez-Sebastià, Miguel Roig-Cayón, Marta Vallés-Rodríguez","doi":"10.1002/cre2.70363","DOIUrl":"https://doi.org/10.1002/cre2.70363","url":null,"abstract":"<p><strong>Objectives: </strong>Printed dental materials have emerged to overcome the limitations associated with the subtractive CAD/CAM technologies. The aim of the present study was to evaluate the optimal adhesive strategy for Permanent Crown Resin, a printed hybrid material.</p><p><strong>Material and methods: </strong>Eighteen specimens of Permanent Crown Resin were divided into three groups of six blocks each, measuring 30 × 30 × 10 mm. A specific surface pretreatment was performed, involving two groups undergoing hydrofluoric acid etching (60 s) and sandblasting (20 s), whereas the third group remained devoid of any pretreatment. Comprehensive surface characterization was achieved using a scanning electron microscope (SEM) at magnifications of 500× and 1000×. Each of the main groups was further divided into three subgroups, each containing two blocks. The paired blocks within each subgroup were then cemented to each other using different cementation protocols: a dual-cure self-adhesive resin cement RelyX^TM Unicem 2, a dual-cure adhesive ExciTE® F DSC, followed by dual-cure resin cement Variolink® Esthetic DC, and a light-cure universal adhesive Scotchbond^TM Universal in combination with preheated composite Filtek^TM Supreme XTE. Subsequently, the specimens were sectioned, and 20 sound micro-bars were obtained from each specimen (n = 180). Each micro-bar was subjected to microtensile bond strength (μTBS) testing at a crosshead speed of 0.5 mm/min.</p><p><strong>Results: </strong>SEM analysis revealed a smooth surface in the control group, whereas the HF group showed numerous micropores. The sandblasting group showed the roughest surface. Statistical analysis was performed using two-way ANOVA, and a p-value of ≤ 0.05 was considered statistically significant. Regarding the μTBS test, sandblasting pretreatment combined with RelyX^TM Unicem 2 demonstrated higher bond strength than the other groups (60.23 MPa). The predominant mode of failure across all groups was adhesive.</p><p><strong>Conclusions: </strong>Within the limitations of this study, sandblasting in conjunction with RelyX^TM Unicem 2 provided the highest bond strength for Permanent Crown Resin.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70363"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147764106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Suturing and Adhesive Fixation on Free Gingival Graft Stability: An Ex-Vivo Porcine-Model Study.","authors":"Kevimy Agossa, Khushboo Kalani, Parham Hazrati, Chi-Fan Chen, Chen-Lin Tsai, Dumitru Chele, Abdusalam E Alrmali, Hom-Lay Wang","doi":"10.1002/cre2.70345","DOIUrl":"https://doi.org/10.1002/cre2.70345","url":null,"abstract":"<p><strong>Objectives: </strong>Clinician expertise influences approach choices, amidst ongoing research on suturing techniques' biomechanical effects on graft stability. This study evaluated different suturing methods and cyanoacrylate adhesive for FGG fixation with a porcine mandible model.</p><p><strong>Material and methods: </strong>Ninety-four FGG procedures were performed on juvenile pig mandibles. Eight fixation methods were evaluated: seven suturing techniques-Cross Compression (CC), Vertical Circumferential Compression (VCC), Horizontal (H), CC + H, VCC + H, Miller (MIL), Holbrook and Ochsenbein (Chan et al.)-and cyanoacrylate adhesive (GLU). Outcomes measured included marginal tension (vertical displacement force) and lateral tension (horizontal displacement) at the graft margin.</p><p><strong>Results: </strong>The HOC technique demonstrated the highest marginal stability (3.82 (SD 1.35 N) and compressive resistance (0.76 (SD 0.48 N). CC + H and VCC + H also showed significantly enhanced marginal stability. Compared to MIL, HOC exhibited superior compressive resistance (p = 0.001), while both HOC and CC + H achieved significantly greater marginal tension (p < 0.05). Graft dimensions influenced outcomes: greater graft height improved compressive resistance, while increased length reduced marginal stability with horizontal sutures. Mean suturing time was 4.84 min (SD 1.55); CC was faster than MIL (p = 0.03), whereas HOC, CC + H, and VCC + H required significantly more time (p < 0.01). GLU achieved marginal stability (3.45 (SD 3.2 N) comparable to HOC.</p><p><strong>Conclusions: </strong>Within the limitations of this ex vivo study, suturing technique and graft dimensions significantly affect the FGG's biomechanical stability. Holbrook and Ochsenbein's suturing technique achieved the highest marginal stability and compressive resistance. Furthermore, tissue adhesive demonstrated comparable performance to conventional sutures, supporting its potential as an alternative fixation method.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70345"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147764129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of RUNX2 Variants With Craniofacial-Dental Phenotypes in Cleidocranial Dysplasia.","authors":"Pintu-On Chantarawaratit, Sermporn Thaweesapphithak, Pisha Pittayapat, Thantrira Porntaveetus","doi":"10.1002/cre2.70351","DOIUrl":"10.1002/cre2.70351","url":null,"abstract":"<p><strong>Objectives: </strong>Cleidocranial dysplasia (CCD) is a rare RUNX2-related skeletal disorder characterized by craniofacial anomalies and skeletal Class III malocclusion. However, the relationship between RUNX2 variant type and phenotype severity remains unclear. This study aimed to evaluate the association between RUNX2 variant types and the severity of skeletal Class III malocclusion and dental anomalies in CCD.</p><p><strong>Material and methods: </strong>This cross-sectional study included 11 unrelated Thai CCD probands and 14 relatives who underwent exome or genome sequencing. RUNX2 variants were classified as non-truncating or truncating/structural according to ACMG-AMP criteria. Nine genetically confirmed individuals met the inclusion criteria for radiographic analysis. Craniofacial parameters and dental anomalies were assessed using lateral cephalograms and cone-beam computed tomography. Group comparisons were performed using nonparametric tests and correlation analysis.</p><p><strong>Results: </strong>Truncating/structural variants were associated with shorter cranial base length, smaller SNA, and reduced nasolabial angles compared with non-truncating variants. The variant type correlated with these parameters. Skeletal Class III malocclusion was present in both groups, predominantly due to mandibular prognathism. No significant association was found between variant type and the burden or distribution of dental anomalies.</p><p><strong>Conclusions: </strong>RUNX2 variant type influences cranial base and maxillary morphology, contributing to skeletal Class III severity in CCD, whereas dental anomalies appear independent of variant type.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70351"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experience and Preparedness of Primary Care Dentistry Residents in Dental Care Delivery to Rural Communities in South Texas.","authors":"Rochisha Singh Marwaha, Sohini Dhar, Suman Challa","doi":"10.1002/cre2.70331","DOIUrl":"https://doi.org/10.1002/cre2.70331","url":null,"abstract":"<p><strong>Objectives: </strong>To assess dental residents' self-perceived preparedness to provide dental care and their experiences delivering care in rural communities in South Texas.</p><p><strong>Materials and methods: </strong>This cross-sectional study evaluates advanced education in general dentistry (AEGD, n = 19) and pediatric dentistry (PD, n = 20) residents' preparation for rural dentists and experiences providing dental care in rural South Texas. Residents participated in a rural dental practice management workshop, followed by clinical rotations at rural community health centers (CHCs) in South Texas. At the conclusion of each CHC rotation, residents completed a Resident's Online Activity Report (ROAR) reflecting on their preparation for rural dental practice, ability to manage patients with complex healthcare needs, and understanding of perceived barriers to care and access issues in rural communities. Descriptive analyses were conducted to summarize residents' patient encounters, preparation to treat patients with complex healthcare needs, and evaluation of rural clinical rotation experience. Open-ended responses were summarized narratively.</p><p><strong>Results: </strong>Primary care dentistry residents participated in 78 rural rotations and completed 74 ROARs (94.9% rate). AEGD residents self-reported (rating system: 1 = no preparation, 4 = excellent preparation) that they were best prepared to treat dental pain (3.54) and manage patients with tobacco use (3.17), while PD residents cited being well prepared to manage early childhood caries (3.76) and dental and facial trauma (3.68). Overall, PD residents (4.19) had a higher mean rating for clinical rotations at rural CHCs than AEGD (4.01). Residents indicated that the most useful parts of the rotations were the formulation of treatment plans guided by specialist faculty, using available resources to provide dental care, and understanding challenges to receiving dental care in rural areas.</p><p><strong>Conclusions: </strong>Didactic education in rural dental practice combined with clinical rotations at rural CHCs across South Texas, improved primary care dentistry residents' understanding of challenges faced by rural populations and enhanced their clinical and patient management skills, better preparing them to care for populations with high burden of oral disease and complex healthcare needs.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70331"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147764054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning-Enhanced Resonance Frequency Analysis for Dental Implant Stability Assessment.","authors":"Zheng Cao, Bi Zhao","doi":"10.1002/cre2.70342","DOIUrl":"10.1002/cre2.70342","url":null,"abstract":"<p><strong>Objectives: </strong>Accurate assessment of dental implant stability is critical for predicting osseointegration outcomes and guiding clinical decision-making. Resonance frequency analysis (RFA) is a widely adopted non-invasive method for measuring implant stability quotient (ISQ); however, signal acquisition noise frequently compromises measurement reliability, leading to variable ISQ readings. This study aimed to develop and evaluate a deep learning-enhanced RFA framework integrating a denoising convolutional neural network (CNN) with a metadata-aware prediction network to improve ISQ estimation accuracy and signal quality.</p><p><strong>Material and methods: </strong>A retrospective dataset of 100 implants (300 signal samples; three acquisitions per implant) was analyzed. The framework comprised: (1) a denoising CNN to suppress signal contamination and improve signal-to-noise ratio (SNR), and (2) a metadata-aware prediction network estimating ISQ from denoised signals and implant-specific parameters (bone density category and insertion torque). Performance was evaluated on a held-out test set (20 implants, 60 samples) using MAE, RMSE, R², and tolerance accuracy within ±3 ISQ units, and compared against a traditional RFA baseline.</p><p><strong>Results: </strong>The denoising network reduced noise by up to 85% and improved mean SNR from 12.3 dB to 22.8 dB. The proposed model achieved MAE of 1.85 ISQ, RMSE of 2.40 ISQ, R² of 0.91, and tolerance accuracy of 92% within ±3 ISQ, outperforming the traditional baseline (MAE 2.65; RMSE 3.35; R² 0.83; tolerance accuracy 77%).</p><p><strong>Conclusions: </strong>The deep learning-enhanced RFA framework substantially improved signal quality and ISQ prediction accuracy over traditional RFA methods, supporting its potential in clinical implant stability monitoring. The framework should currently be regarded as a proof-of-concept; multi-center prospective validation incorporating real-world noise profiling and clinical outcome assessment is required before clinical deployment.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70342"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13140712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Platelet-Rich Platelet Aggregates (PRFs) in Soft Tissue Repair in Periodontics: A Systematic Review.","authors":"Ilan Hudson Gomes de Santana, Ozawa Brasil de Júnior, Anderson Jara Ferreira, Katia Caetana Pereira, José Marcos Pereira Júnior, Erick Andres Alpaca Zevallos, Gustavo Ramiro Rojas Manrique, Jaime S Gallegos Zanabria, Camila Coelho Guimarães, Ennyo Sobral Crispim da Silva","doi":"10.1002/cre2.70327","DOIUrl":"https://doi.org/10.1002/cre2.70327","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate, through a systematic review, the clinical effectiveness of platelet-rich fibrin (PRF) in periodontal soft tissue repair compared with conventional regenerative techniques.</p><p><strong>Material and methods: </strong>This systematic review followed PRISMA 2020 guidelines and was registered in PROSPERO (CRD42024617061). Searches were conducted in PubMed, Scopus, Web of Science, ScienceDirect, and LILACS, complemented by gray literature sources. Randomized clinical trials evaluating PRF or its derivatives in periodontal soft tissue repair were included. From 996 identified records, 28 studies met the eligibility criteria and were included in the qualitative analysis.</p><p><strong>Results: </strong>PRF demonstrated clinical outcomes comparable to conventional techniques such as connective tissue grafts and other biomaterials for root coverage and periodontal regeneration. In addition, PRF was associated with reduced surgical morbidity, lower postoperative discomfort, and shorter surgical time.</p><p><strong>Conclusions: </strong>PRF represents a promising autologous biomaterial for periodontal soft tissue repair, providing clinical outcomes similar to conventional grafting techniques with lower morbidity. Further well-designed clinical trials with standardized protocols and long-term follow-up are required.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70327"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13122118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Obstructive Sleep Apnea and Periodontitis: A Systematic Umbrella Review.","authors":"Mohammed Khalid Mahmood, Shahen Hiwa Omer, Sarhang Sarwat Gul, Yad Mariwan Mohammed Amin, Herve Tassery, Delphine Tardivo, Romain Lan","doi":"10.1002/cre2.70300","DOIUrl":"https://doi.org/10.1002/cre2.70300","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to evaluate the association between obstructive sleep apnea (OSA) and periodontitis using an umbrella meta-analysis (MA).</p><p><strong>Methods: </strong>PubMed, MEDLINE, Web of Science, Scopus, Embase, and Google Scholar databases were systematically searched before December 2025. MAs reporting odds ratios (OR) for the association between OSA and periodontitis were included. Methodological quality was assessed using AMSTAR-2, and certainty of evidence was evaluated using GRADE. Random-effects MA was performed. Egger's test and trim-and-fill analysis were used to assess the publication bias.</p><p><strong>Results: </strong>Seven MAs encompassing over 225,000 participants were included. A significant association was found between OSA and periodontitis (OR 1.96, 95% CI 1.68-2.29, p < 0.0001), with substantial heterogeneity (I² = 69.5%). After using trim-and-fill method to adjust for publication bias, the association strengthened (OR 2.24, 95% CI 1.92-2.60, p < 0.0001). A dose-response pattern emerged, with severe OSA showing higher odds (OR 2.25) compared to mild-moderate OSA (OR 1.82), though not statistically different (p = 0.44). The association remained consistent among different study qualities and sample sizes. The GRADE assessment rated the certainty of evidence of the main outcome as low due to heterogeneity and publication bias. Further, a high overlap ratio of 26.8% was observed among the included MAs.</p><p><strong>Conclusions: </strong>The results of this review suggest a significant association between OSA and periodontitis, with a greater association of severe OSA with periodontitis. Future studies should examine the impact of periodontal therapy on OSA severity and vice versa.</p><p><strong>Prospero registration: </strong>CRD420251241137.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 2","pages":"e70300"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147764062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}