Zhu Xiufeng, Wang Miao, Zhou Huixia, Xu Boya, Chang Xiaofeng, He Longlong
{"title":"低强度热刺激促进兔植入物早期骨整合的临床前研究。","authors":"Zhu Xiufeng, Wang Miao, Zhou Huixia, Xu Boya, Chang Xiaofeng, He Longlong","doi":"10.1002/cre2.70223","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>This study is the first to integrate 3D finite element modeling, in vitro validation, and preclinical animal experiments to determine the efficacy of low-intensity thermal stimulation (LITS) in enhancing dental implant osseointegration. The study seeks to provide experimental evidence for applying thermal stimulation as a possible approach to enhance osseointegration.</p>\n </section>\n \n <section>\n \n <h3> Material and Methods</h3>\n \n <p>A 3D finite element implant-femur model and in vitro implant-bone system were developed to simulate heat distribution. LITS conditions (50°C/5 s) were validated to avoid exceeding the osteoblast safety threshold (47°C). Eighteen rabbits received femoral implants divided into: control (no heating), T1 (single 50°C/5 s heating cycle), and T2 (three cycles). Outcomes included implant stability (IST), reverse torque, bone volume fraction (BV/TV), and histomorphometric osseointegration rate at 6 weeks.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Finite element and in vitro analyses confirmed 50°C/5 s as the optimal protocol, maintaining implant surface temperatures ≤ 46.3°C and complete thermal recovery within 1 min. T1 significantly increased in vivo rabbit model reverse torque (<i>p</i> < 0.05) and BV/TV (<i>p</i> < 0.05), while T2 showed no BV/TV improvement. Both T1 and T2 exhibited higher osseointegration rates (<i>p</i> < 0.05). Implant stability (IST) remained unchanged across groups (<i>p</i> > 0.05).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>LITS at 50°C/5 s safely enhances early osseointegration in rabbits, increasing biomechanical anchorage and peri-implant bone formation. This study provides preliminary experimental evidence for the potential of thermal application in enhancing implant osseointegration.</p>\n </section>\n </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 5","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70223","citationCount":"0","resultStr":"{\"title\":\"Low Intensity Thermal Stimulation to Enhance Early Osteointegration in Implants: A Preclinical Study in Rabbits\",\"authors\":\"Zhu Xiufeng, Wang Miao, Zhou Huixia, Xu Boya, Chang Xiaofeng, He Longlong\",\"doi\":\"10.1002/cre2.70223\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objectives</h3>\\n \\n <p>This study is the first to integrate 3D finite element modeling, in vitro validation, and preclinical animal experiments to determine the efficacy of low-intensity thermal stimulation (LITS) in enhancing dental implant osseointegration. The study seeks to provide experimental evidence for applying thermal stimulation as a possible approach to enhance osseointegration.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Material and Methods</h3>\\n \\n <p>A 3D finite element implant-femur model and in vitro implant-bone system were developed to simulate heat distribution. LITS conditions (50°C/5 s) were validated to avoid exceeding the osteoblast safety threshold (47°C). Eighteen rabbits received femoral implants divided into: control (no heating), T1 (single 50°C/5 s heating cycle), and T2 (three cycles). Outcomes included implant stability (IST), reverse torque, bone volume fraction (BV/TV), and histomorphometric osseointegration rate at 6 weeks.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Finite element and in vitro analyses confirmed 50°C/5 s as the optimal protocol, maintaining implant surface temperatures ≤ 46.3°C and complete thermal recovery within 1 min. T1 significantly increased in vivo rabbit model reverse torque (<i>p</i> < 0.05) and BV/TV (<i>p</i> < 0.05), while T2 showed no BV/TV improvement. Both T1 and T2 exhibited higher osseointegration rates (<i>p</i> < 0.05). Implant stability (IST) remained unchanged across groups (<i>p</i> > 0.05).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>LITS at 50°C/5 s safely enhances early osseointegration in rabbits, increasing biomechanical anchorage and peri-implant bone formation. This study provides preliminary experimental evidence for the potential of thermal application in enhancing implant osseointegration.</p>\\n </section>\\n </div>\",\"PeriodicalId\":10203,\"journal\":{\"name\":\"Clinical and Experimental Dental Research\",\"volume\":\"11 5\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70223\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and Experimental Dental Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cre2.70223\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Dental Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cre2.70223","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Low Intensity Thermal Stimulation to Enhance Early Osteointegration in Implants: A Preclinical Study in Rabbits
Objectives
This study is the first to integrate 3D finite element modeling, in vitro validation, and preclinical animal experiments to determine the efficacy of low-intensity thermal stimulation (LITS) in enhancing dental implant osseointegration. The study seeks to provide experimental evidence for applying thermal stimulation as a possible approach to enhance osseointegration.
Material and Methods
A 3D finite element implant-femur model and in vitro implant-bone system were developed to simulate heat distribution. LITS conditions (50°C/5 s) were validated to avoid exceeding the osteoblast safety threshold (47°C). Eighteen rabbits received femoral implants divided into: control (no heating), T1 (single 50°C/5 s heating cycle), and T2 (three cycles). Outcomes included implant stability (IST), reverse torque, bone volume fraction (BV/TV), and histomorphometric osseointegration rate at 6 weeks.
Results
Finite element and in vitro analyses confirmed 50°C/5 s as the optimal protocol, maintaining implant surface temperatures ≤ 46.3°C and complete thermal recovery within 1 min. T1 significantly increased in vivo rabbit model reverse torque (p < 0.05) and BV/TV (p < 0.05), while T2 showed no BV/TV improvement. Both T1 and T2 exhibited higher osseointegration rates (p < 0.05). Implant stability (IST) remained unchanged across groups (p > 0.05).
Conclusion
LITS at 50°C/5 s safely enhances early osseointegration in rabbits, increasing biomechanical anchorage and peri-implant bone formation. This study provides preliminary experimental evidence for the potential of thermal application in enhancing implant osseointegration.
期刊介绍：
Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.
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