Clinical and Experimental Dental Research最新文献

{"title":"Oral Health Interventions in Children With Autism Spectrum Disorder: A Systematic Review and Meta-Analysis of Interventional Studies","authors":"Iman Rajaei,&nbsp;Azadeh Babaei,&nbsp;Shirin Djalalinia,&nbsp;Mostafa Qorbani","doi":"10.1002/cre2.70258","DOIUrl":"10.1002/cre2.70258","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Children with autism spectrum disorder (ASD) exhibit a higher prevalence of oral health diseases. These oral health issues significantly impair quality of life and increase caregiver burden. Despite emerging interventions like specialized behavioral guidance and caregiver training, a comprehensive evaluation of their effectiveness remains absent. This systematic review and meta-analysis was designed to pool the effectiveness of Oral health interventions in children with ASD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>In this systematic review, some international databases, including PubMed, ISI/WOS, CENTRAL, and Scopus, were searched via appropriate keywords until January 1, 2025. All clinical trials that assessed the effect of interventions on oral health indices including Simplified Oral Hygiene Index (OHI-S), plaque index (PI), and gingival index (GI) without any language or time restrictions were included in the study. Search strategy process, screening, data extraction, and quality assessment were performed by two experts independently. Heterogeneity between studies was assessed using I² and Q-Cochrane test. Random effect meta-analysis was performed to pool the standardized mean difference (SMD) with 95% confidence intervals (CIs).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Overall, 27 studies with 1918 participants were included in this study. Interventions are categorized into the educational (visual, verbal, booklet/leaflet) and procedural approaches. Random effect meta-analysis showed significant improvements for PI (SMD = −0.73, 95% CI: [−1.02 to −0.44]) and OHI-S (SMD = −1.44, 95% CI: [−2.79 to −0.08]), with video interventions notably effective for PI (SMD = −0.69, 95% CI: [−1.2 to −0.14]). The GI index also improved (SMD = −0.74, 95% CI: [−1.34 to −0.14]).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Visual pedagogy, particularly videos, and parental involvement significantly improved oral health in children with ASD. Video-based interventions should be added to traditional methods such as verbal or picture-based approaches to improve oral health interventions in ASD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI in Root Canal Morphology: A Detailed Bibliometric Analysis of Research Trends and Global Contributions","authors":"Waseem Hassan,&nbsp;Vini Mehta,&nbsp;Niher Tabassum Singdha,&nbsp;Mohmed Isaqali Karobari","doi":"10.1002/cre2.70269","DOIUrl":"10.1002/cre2.70269","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Artificial Intelligence (AI) has emerged as a transformative tool in various scientific disciplines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The study aims to provide a comprehensive bibliometric analysis of the literature on AI and root canal morphology, examining publication trends, author contributions, and thematic focus areas.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Data were retrieved from the Scopus database using the search string “Artificial Intelligence” OR “AI” AND “Root” OR “Root canal morphology,” covering publications from 1997 to 2024. Only articles and reviews were included. Vosviewer software was employed to visualize co-authorship networks and perform co-word analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis encompassed 81 papers, 433 authors, and 274 departmental contributions across 31 countries. The documents were published in 52 journals. The analysis revealed a global collaboration network with significant contributions from diverse institutions and countries. By co-word analysis, the focus of the 81 papers is categorized into 25 distinct research areas. The thematic analysis identified key research areas and emerging trends within the literature. The findings highlight AI applications' increasing interest and interdisciplinary nature in root canal morphology research.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study provides a detailed overview of the research landscape concerning AI and root canal morphology. It identified leading contributors and institutions and presented a structured view of research themes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Pellicle on Biofilm Formation in a Supragingival Biofilm Model","authors":"Shengjile Deari,&nbsp;Monika Gothwal,&nbsp;Kay Gränicher,&nbsp;Thomas Thurnheer,&nbsp;Thomas Attin,&nbsp;Lamprini Karygianni","doi":"10.1002/cre2.70276","DOIUrl":"10.1002/cre2.70276","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Oral biofilms initiate with the formation of an acquired pellicle on dental surfaces, a thin layer of salivary glycoproteins that provides a substrate for microbial adhesion. This study aimed to assess the necessity of a preformed pellicle for biofilm growth in vitro by analyzing the development of a standardized six-species biofilm, comprising <i>Actinomyces oris</i>, <i>Veillonella dispar</i>, <i>Fusobacterium nucleatum</i>, <i>Streptococcus sobrinus</i>, <i>Streptococcus oralis</i>, and <i>Candida albicans</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Biofilms were cultivated on bovine enamel discs under two conditions: (1) precoated with human saliva to simulate a pellicle and (2) without a preformed pellicle. Colony-forming units (CFUs) of each microbial species were quantified after incubation in either human saliva or a NaCl-based medium at 16 and 64 h.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis revealed no significant differences in CFU counts between discs with or without a preformed pellicle, regardless of whether biofilms were grown in human saliva or NaCl medium, with one exception: S. oralis in pellicle/NaCl (6.7 Log₁₀) medium at 16 h showed a slight decrease in the absence of a pellicle (5.9 Log₁₀).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings suggest that microbial adhesion and subsequent biofilm development occurred independently of an initial pellicle. The preformed salivary pellicle does not seem to play a significant role in the initial development of this in vitro biofilm model. Biofilm testing in laboratory settings, especially for studies on antimicrobial efficacy, could be simplified, as pellicle formation may not be an essential requirement. Although no significant differences in biofilm development were observed between pellicle and no-pellicle conditions, the growth medium may have influenced pellicle interactions, warranting further investigation of media effects on pellicle formation. Existing assumptions about pellicle dependence in biofilm formation are challenged, and suggest that in vitro models without a pellicle may still provide valid platforms for studying biofilms and testing antimicrobial agents effectively.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of miR-516a-5p Promotes Erosive Oral Lichen Planus: In Vitro Study Based on Bioinformatics Analyses","authors":"Yan Chen,&nbsp;Chen‑xi Li,&nbsp;Rui Xue,&nbsp;Zhao-xia Cong,&nbsp;Yi Bian,&nbsp;Yuan Liu","doi":"10.1002/cre2.70270","DOIUrl":"10.1002/cre2.70270","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to investigate the differentially expressed microRNAs in erosive oral lichen planus, followed by analyzing how the overexpression of identified miR-516a-5p influences human oral mucosal fibroblasts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>High-throughput sequencing using tissues from patients and healthy individuals identified varying microRNA expression profiles in erosive oral lichen planus tissues. Bioinformatics analysis subsequently revealed the enriched pathways and targeted genes involved. In vitro experiments were performed to confirm the validity of bioinformatic findings.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 82 microRNAs were differentially expressed in erosive oral lichen planus tissues. These microRNAs are mainly linked to T helper cell differentiation that enriched in MAPK signaling pathways, as well as targeting mRNAs like MAPK11. Overexpression of miR-516a-5p resulted in a statistically significant decrease in MAPK11 mRNA level in human oral mucosal fibroblasts. Overexpression of miR-516a-5p increased TNF-α and IFN-γ levels, whereas it decreased IL-4, IL-6, IL-10, and IL-13 levels. Overexpression of miR-516a-5p enhanced both the proliferation and migration of human oral mucosal fibroblasts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The miR-516a-5p overexpression in patients with erosive oral lichen planus may contribute to the imbalance of T helper 1/2 cell-associated inflammatory cytokine expression in human oral mucosal fibroblasts by targeting MAPK11 mRNA, promoting their proliferative and migratory capacities.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FOXP3+ T Cells—An Emerging Evidence in Periodontitis Therapeutics","authors":"Revan Birke Koca Ünsal,&nbsp;Akira Hasuike,&nbsp;Tamer Badawy,&nbsp;Farah Asa'ad,&nbsp;Bruno Špiljak,&nbsp;Monal Yuwanati,&nbsp;Jelena Roganović,&nbsp;Yinli Liu,&nbsp;Carel Brigi,&nbsp;Danilo Milanes Zambrano,&nbsp;Akhilanand Chaurasia","doi":"10.1002/cre2.70263","DOIUrl":"10.1002/cre2.70263","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To review interaction of FOXP3+ regulatory T cells with Th17 cells in determining the progression of periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methodology</h3>\u0000 \u0000 <p>Literature review pertaining to FOXP3+ regulatory T cells, Th17 cells, and periodontitis was analyzed. Descriptive summary is presented.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>FOXP3+ regulatory T cells (Tregs) play an essential role in maintaining immune homeostasis and modulating inflammatory responses. The balance between Tregs and pro-inflammatory Th17 cells is crucial in determining the progression of periodontitis, a chronic immune-mediated inflammatory disease. While Tregs are responsible for suppressing excessive immune activation and preventing tissue destruction, an imbalance favoring Th17 cells leads to increased osteoclastic activity and alveolar bone loss through IL-17 and RANKL signaling. The inflammatory microenvironment in periodontitis compromises FOXP3+ Treg stability and function, thereby allowing unregulated immune responses that exacerbate periodontal tissue breakdown. Recent studies suggest that strategies aimed at enhancing Treg-mediated immune regulation, such as IL-2 supplementation, all-trans retinoic acid (ATRA), IL-33 administration, and CCL22-mediated recruitment, could mitigate periodontal inflammation and preserve alveolar bone integrity. Furthermore, systemic conditions like diabetes and obesity play a significant role in disrupting Treg function by promoting a pro-inflammatory environment, impairing immune regulation, and exacerbating immune dysregulation. This dysfunction weakens the protective role of Tregs, leading to an intensified inflammatory response that accelerates periodontal tissue destruction and alveolar bone loss.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Understanding the mechanisms governing FOXP3+ Treg stability and their interaction with pathogenic Th17 responses is essential for developing targeted immunomodulatory therapies. Future research should focus not only on selectively expanding Tregs but also on translational strategies such as adoptive Treg transfer and IL-17 inhibition, while carefully balancing efficacy and the risk of systemic immunosuppression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Dental Anatomy: An Ex Vivo Comparative Study Between Photon-Counting Detector CT and Cone-Beam CT","authors":"Adib Al-Haj Husain,&nbsp;Fabian Benz,&nbsp;Victor Mergen,&nbsp;Silvio Valdec,&nbsp;Sebastian Winklhofer,&nbsp;Hatem Alkadhi,&nbsp;Harald Essig,&nbsp;Thomas Frauenfelder,&nbsp;Rubens Spin-Neto,&nbsp;Bernd Stadlinger","doi":"10.1002/cre2.70266","DOIUrl":"10.1002/cre2.70266","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aim of this ex vivo study was to assess the performance of photon-counting detector computed tomography (PCD-CT) compared with cone-beam computed tomography (CBCT) at equivalent radiation doses for detecting and evaluating mandibular and dental anatomical structures in porcine cadavers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>This intermodal comparative study evaluated imaging protocols at three radiation dose levels (high: 360 μSv, standard: 145 μSv, low: 20 μSv) in six porcine cadaver heads, analyzing 12 CBCT and 18 PCD-CT volumes. Two blinded observers assessed image quality, artifact susceptibility, and diagnostic interpretability using a 5-point Likert scale (5 = highest, 1 = lowest). Statistical analysis included descriptive statistics and interobserver reliability, assessed by weighted kappa (κ) analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>PCD-CT either matched or outperformed CBCT at standard- and low-dose protocols, demonstrating superior anatomy coverage, density, contrast, and less artifact susceptibility. High- and standard-dose protocols achieved perfect scores 5 (SD = 0). At low-dose levels, PCD-CT showed slightly lower scores but still outperformed CBCT. PCD-CT showed minimal artifacts, with no significant artifacts in high and standard doses. Inter- and intra-reader reliability was higher for PCD-CT (κ: 0.694–1; <i>p</i> &lt; 0.001) compared to CBCT (0.55–0.916; <i>p</i> &lt; 0.001), with the difference being largest at low doses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>PCD-CT outperforms CBCT in diagnostic interpretability and artifact reduction across various radiation dose levels, offering a promising alternative for dentomaxillofacial imaging that aligns with the ALADAIP principle.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning Models for Evaluating the Anatomical Relationship Between Posterior Maxillary Teeth and Maxillary Sinus in Panoramic Radiographs","authors":"Akram Fallah,&nbsp;Parisa Soltani,&nbsp;Mojdeh Mehdizadeh,&nbsp;Mostafa Riahi Farsani,&nbsp;Mahsa Moannaei,&nbsp;Seyed Amir Hossein Ourang,&nbsp;Maryam Hossaini,&nbsp;Gianrico Spagnuolo,&nbsp;Carlo Rengo","doi":"10.1002/cre2.70264","DOIUrl":"10.1002/cre2.70264","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Accurate evaluation of the anatomical relationship between posterior maxillary teeth and the maxillary sinus is critical in dental treatments such as orthodontics, surgery, and implantology. This study investigates the efficacy of deep learning models applied to panoramic images for predicting this anatomical relationship.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 300 panoramic images and 1760 cropped slices were collected and converted to PNG format at a resolution of 512 × 512 pixels. Three convolutional neural network architectures—VGG, ResNet, and ResNeXt—were trained and evaluated. The dataset was split into training (80%), validation (10%), and testing (10%) sets. Performance metrics included accuracy, precision, recall, F1 score, ROC-AUC, and confusion matrices.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The VGG model achieved an accuracy, precision, recall, and F1 score of 0.84, with an ROC-AUC of 0.89. ResNet and ResNeXt demonstrated superior performance with all metrics at 0.88 and ROC-AUC values of 0.93 and 0.94, respectively. Both ResNet and ResNeXt showed signs of overfitting after 30–50 epochs, suggesting the need for early stopping or regularization. False positives mainly occurred in second molars falsely classified as contacting the sinus.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Deep learning models, particularly ResNet and ResNeXt, provide reliable assessment of the anatomical relationship between posterior maxillary teeth and the maxillary sinus using panoramic radiographs. These models can serve as efficient diagnostic aids when CBCT is unavailable, improving clinical decision-making in panoramic images.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Will L-PRF Be the Future of Endodontic Microsurgery? A Series of Case Reports","authors":"Natrah Ahmad Fuad,&nbsp;Panagiotis Pitros,&nbsp;Graeme Brown,&nbsp;Eleni Besi","doi":"10.1002/cre2.70198","DOIUrl":"10.1002/cre2.70198","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This case series aimed to evaluate the healing potential of apical tissues with large periapical radiolucencies (&gt; 10 mm) after apical microsurgery with L-PRF. The secondary objectives were to evaluate L-PRF's benefits and adverse effects as well as to aid in the development of a clinical protocol.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This case series was conducted in accordance with the Preferred Reporting Items for Case Reports in Endodontics (PRICE) 2020 guidelines. Thirteen patients with persistent endodontic infections, unresponsive to nonsurgical root canal treatment/retreatment, were treated at the Restorative and Oral Surgery Departments with endodontic microsurgery. L-PRF preparation followed Choukroun et al. (2001) and the L-PRF 2018 guidelines under the supervision of an experienced consultant. Postoperative follow-up included a phone call at 24 h to assess pain, swelling, and daily functions. Sutures were removed at 7 days, and a 6-month clinical and radiographic review was conducted. The clinical assessment included patient-reported symptoms and extraoral and intraoral examinations. Periapical radiographs were assessed for periapical healing based on Rud et al.'s (1972) radiographic criteria. Radiographs were reviewed by one clinician under standardized conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Histopathological analyses identified 76.9% (<i>n</i> = 10) radicular cysts and 23.0% (<i>n</i> = 3) periapical granulomas from the 13 cases. At the 6-month review, 76.9% (<i>n</i> = 10) showed incomplete healing, 15.4% (<i>n</i> = 2) demonstrated complete healing, and 7.7% (<i>n</i> = 1) had incomplete healing at 4 months. All patients remained asymptomatic with no reported complaints. Radiographic assessments showed a significant reduction in the size of periapical radiolucency in all cases. At 24 h, 69.2% (<i>n</i> = 9) reported no pain, while mild pain was noted in 15.4% (<i>n</i> = 2). Swelling was observed in 69.2% (<i>n</i> = 9) and absent in 15.4% (<i>n</i> = 2), with missing records for 15.4% (<i>n</i> = 2).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>L-PRF appears beneficial in endodontic microsurgery. However, larger, low-bias studies with extended follow-up periods are needed for definitive conclusions on its application.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study on the Fracture Resistance of CAD/CAM–Fabricated Single-Piece Post-Crowns","authors":"Ali Erdem,&nbsp;Mehmet Selim Bilgin,&nbsp;Ibrahim Ersoy,&nbsp;Erhan Dilber,&nbsp;Ebru Nur Işık,&nbsp;Tan Fırat Eyüboğlu,&nbsp;Mutlu Özcan","doi":"10.1002/cre2.70247","DOIUrl":"10.1002/cre2.70247","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Recently, CAD/CAM materials have become popular in dental clinics; however, information about their fracture resistance as post-crowns remains limited. This study compared the initial fracture resistance of potential single-piece post-crown materials made with CAD/CAM milling to sound teeth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Fifty freshly extracted, non-carious human central incisor teeth underwent endodontic treatment. The roots were then randomly divided into five groups based on the post systems: the control group included teeth filled only with gutta-percha. Monoblock post-crowns were made using four different systems for the other groups: LDS (IPS e.max CAD®, lithium disilicate glass-ceramic), YTZP (inCoris ZI®, Yttrium tetragonal zirconia polycrystal), RNC (Lava Ultimate®, resin-based nanoceramic), and PICN (VITA Enamic®, polymer-infiltrated ceramic network). The post-crowns were cemented with resin cement and tested with a universal testing machine at a crosshead speed of 1.0 mm/min. Data analysis used one-way ANOVA and multiple comparison post-hoc Tukey tests (<i>α</i> = 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant differences were found between the groups (<i>p</i> &lt; 0.001). The control group exhibited the highest fracture resistance (749.25 ± 225.02 N). YTZP showed similar resistance to the control (<i>p</i> = 0.99) and LDS (447.28 ± 168.72 N, <i>p</i> = 0.081), but was significantly higher than RNC (343.79 ± 157.08 N, <i>p</i> = 0.0051) and PICN (348.78 ± 157.44 N, <i>p</i> = 0.0059). LDS, RNC, and PICN did not differ significantly. YTZP experienced more non-repairable fractures (5/10), while PICN predominantly failed in a repairable manner (9/10).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>All CAD/CAM post-crowns exceeded functional loads for the anterior region. YTZP and LDS demonstrated greater strength, whereas PICN and RNC, despite being weaker, favored repairable failures—highlighting the importance of balancing strength and clinical manageability.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Raman Spectroscopy and Micro-Indentation to Micro-Map the Path and Boundary of NaOCI-Induced Dentine Collagen Changes in an Ex-Vivo Root Canal Irrigation Model","authors":"Yuan Ng,&nbsp;Michele Recchia,&nbsp;Cristina Pereira,&nbsp;Graham Palmer,&nbsp;Laurent Bozec,&nbsp;Kishor Gulabivala","doi":"10.1002/cre2.70262","DOIUrl":"10.1002/cre2.70262","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To apply Raman spectroscopy and micro-indentation to micro-map the path and boundary of NaOCl-induced dentine collagen changes in an ex-vivo root canal irrigation model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Root canals of extracted single-rooted teeth were prepared and irrigated with NaOCl or saline. Four teeth (NaOCl = 3; saline = 1) embedded in epoxy resin and sectioned transversely into discs were Raman-analyzed on coronal surfaces from <i>inter-</i> and <i>intra-tubular</i> dentine in 4 quadrants over 12, 24, and 48 min-acquisition times. Eight additional teeth stratified by root maturity, irrigated with NaOCl (<i>n</i> = 7) or saline (<i>n</i> = 1), sectioned transversely and then embedded, were Raman-analyzed on apical surfaces at 18 equidistant (50 μm) points/quadrant for Amide bands. Micro-indentation of the corresponding facing sectioned surface was correlated with Amide band changes. Generalized linear and non-linear regression models were used for data analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Spectral quality at 24/48 min was similar and better than at 12 min. Inter-tubular but not intra-tubular spectra were masked by fluorescence. Spectral features near the canal lumen ( &lt; 500 μm) showed more significant collagen alteration and varied by tooth/quadrant but decreasingly towards the cemento-dentinal junction (CDJ) without a clear boundary. Significant (<i>p</i> &lt; 0.0001) changes in Amide I/III bands up to 300 μm from the canal and were accompanied by deeper corresponding indentations upto 200 μm. Canal instrumentation had a significant (<i>p</i> &lt; 0.0001) effect on both Amide-I and Amide-III bands.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>NaOCl altered dentinal collagen and reduced microhardness but varied with quadrants/teeth, without a definable boundary; collagen changes were obvious within 300 μm of the canal and microhardness changes within 200 μm but evident to a decreasing extent up to the CDJ.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}