Let-7e as Potential Diagnostic Biomarkers in Early Detection of Peri-Implant Diseases: A Cross-Sectional Study

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2025-05-05 DOI:10.1002/cre2.70044

Nurul Latifah Zainal Abidin, Norjehan Latib, Fouad Hussain Al-Bayaty, Mohd Faizal Hafez Hidayat

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引用次数: 0

Abstract

Objectives

Peri-implant diseases (PID), specifically peri-implant mucositis and peri-implantitis, significantly jeopardize dental implant success. Traditional diagnostic methods, relying on periodontal probing and radiographs, have limitations in precisely diagnosing these conditions. Recent studies suggest that microRNA (miRNA) profiling might be a breakthrough in the early detection of PID. This study aimed to evaluate the expression levels of specific miRNAs—miR-98, miR-145, miR-146a, and let-7e—in individuals with peri-implant mucositis and peri-implantitis compared to a healthy control.

Methods

From January 2022 to October 2023, 19 participants with 38 dental implants were recruited according to established criteria. After clinical and radiographic evaluations, dental implants were grouped as peri-implant health (Group 1), peri-implant mucositis (Group 2), and peri-implantitis (Group 3). Subsequently, peri-implant crevicular fluid (PICF) samples were collected and analyzed through real-time quantitative polymerase chain reaction (qPCR).

Results

Based on the findings, only let-7e biomarkers were consistently detected across all samples, with their expression increased eightfold in peri-implant mucositis and 94-fold in peri-implantitis cases. Kruskal–Wallis's test showed a statistically significant difference in relative gene expression of let-7e between the different groups, H(2) = 25.825, p ≤ 0.001.

Conclusions

Our study indicates that miRNA biomarkers, particularly let-7e, may play a significant role in the development and progression of PID, highlighting the need for further in-depth investigation, with PICF offering a practical, noninvasive sampling method.

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Let-7e作为早期发现种植体周围疾病的潜在诊断性生物标志物：一项横断面研究

目的种植体周围疾病（PID），特别是种植体周围粘膜炎和种植体周围炎，严重危害种植体的成功。传统的诊断方法，依靠牙周探查和x线片，在精确诊断这些疾病方面有局限性。最近的研究表明，microRNA （miRNA）谱分析可能是PID早期检测的一个突破。本研究旨在评估与健康对照相比，特定mirna - mir -98、miR-145、miR-146a和let-7e在种植体周围粘膜炎和种植体周围炎个体中的表达水平。方法于2022年1月至2023年10月，根据既定标准招募19名受试者，种植体38枚。经过临床和影像学评估，种植体分为种植体周围健康组（1组）、种植体周围粘膜炎组（2组）和种植体周围炎组（3组）。随后，采集种植体周围沟液（PICF）样本，通过实时定量聚合酶链反应（qPCR）进行分析。基于研究结果，在所有样本中只有let-7e生物标志物被一致检测到，它们的表达在种植体周围粘膜炎病例中增加了8倍，在种植体周围炎病例中增加了94倍。Kruskal-Wallis检验显示，不同组间let-7e基因相对表达量差异有统计学意义，H(2) = 25.825, p≤0.001。我们的研究表明，miRNA生物标志物，特别是let-7e，可能在PID的发生和进展中发挥重要作用，强调需要进一步深入研究，PICF提供了一种实用的、无创的采样方法。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.