Cell and Tissue Banking最新文献

{"title":"Determinants of extraction efficiency in human amniotic membrane processing: biological, mechanical, and biochemical factors shaping growth factor yield.","authors":"Ozlem Barut Selver, Anil Seyfi Kanbur, Meltem Kocamanoglu, Ilayda Korkmaz, Yilmaz Kadir Atsatan, Mehmet Gurdal, Nuri Yildirim, Jose Mario Wolosin, Gulinnaz Ercan, Timur Köse, Mesut Arici","doi":"10.1007/s10561-026-10229-z","DOIUrl":"10.1007/s10561-026-10229-z","url":null,"abstract":"<p><p>Human amniotic membrane extract (AME) contains growth factors that contribute to epithelial regeneration and immunomodulation. This review synthesizes current evidence and incorporates our recent preliminary experimental data to identify the critical parameters that influence the extraction efficiency of six key growth factors-EGF, HGF, TGF-β, bFGF, KGF, and NGF-from human amniotic membrane obtained via cesarean delivery. A comprehensive literature analysis was combined with empirical observations from our laboratory using cryogenic pulverization, differential buffer systems, protease inhibition strategies, and post-extraction stabilization techniques. Factor-specific biochemical behavior and matrix interactions were evaluated to outline optimized extraction conditions. Extraction efficiency was strongly dependent on: 1. preservation of epithelial and stromal integrity; 2. cryogenic pulverization for maximal cell lysis; 3. buffer composition, particularly the use of trehalose for protein stabilization; 4. heparin or high ionic strength solutions for releasing matrix-bound factors such as HGF, bFGF, and KGF; 5. EDTA-based protease control for NGF; and 6. sonication-assisted liberation of latent TGF-β complexes. Our practical study data confirm that an extraction protocol incorporating phosphate-buffered saline (PBS), trehalose, and cryo-milling substantially enhances the recovery of EGF, NGF, and TGF-β levels, while the addition of protease inhibitors to PBS and cryo-milling improves the concentrations of HGF, FGF, and TGF-β. AME yield is governed by a constellation of process-dependent variables that can be systematically optimized. Integrating biological tissue properties with precise mechanical and biochemical strategies enables the production of standardized, high potency extracts suitable for clinical and biobanking applications. Establishing these optimized conditions may support the development of GMP-compliant workflows and improve the therapeutic consistency of AME-based products.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of allogenic nasal cartilage storage media in terms of biosafety and viability.","authors":"Gülnihal Bozdağ, Didem Aksu, Olcay Eren, Selim Turan, Gökhan Meriç, Gamze Torun Köse","doi":"10.1007/s10561-026-10228-0","DOIUrl":"10.1007/s10561-026-10228-0","url":null,"abstract":"<p><p>Cartilage allotransplantation has been employed for years as an effective remedy for cartilage-related disorders. The storage conditions and duration play a critical role in preserving the quality of fresh human nasal allografts. In this study, human nasal cartilages were stored in a designated medium for 2, 4, 6, 8, 10, and 12 weeks at 4 °C, and their cell viability, biomechanical properties, structural integrity, and sterility were evaluated. Cell viability remained over 70% for a duration of 12 weeks with only a gradual decline over time. Throughout the duration of the experiment, no microbiological contamination was identified in the storage media. The Young's modulus remained above 15 MPa up to 6 weeks, indicating maintained mechanical integrity during early storage. Histological analysis demonstrated preservation of chondrocyte morphology and extracellular matrix structure up to 8 weeks, with gradual structural changes observed thereafter. These results indicate that the storage medium successfully maintains nasal cartilage viability, sterility, and functional characteristics for prolonged durations, marking the longest reported storage period at 4 °C. The results emphasize the possible use of nasal cartilage as a clinically viable substitute for articular cartilage in transplantation applications.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148027547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A practical and efficient technique for corneal allogenic ring segment implantation: prolene suture-guided insertion.","authors":"Yusuf Koçluk, Muzaffer Talha Albayrak, Burcu Kasım, Burak Özkan","doi":"10.1007/s10561-026-10227-1","DOIUrl":"10.1007/s10561-026-10227-1","url":null,"abstract":"<p><p>To describe a practical and efficient prolene suture-guided technique for corneal allogenic intrastromal ring segment (CAIRS) implantation and to evaluate intraoperative difficulties and complications associated with the procedure. Medical records and surgical videos of patients who underwent CAIRS for keratoconus were retrospectively reviewed. All procedures were performed by the same surgeon using standardized femtosecond laser parameters. Donor stromal rings were prepared from residual corneal tissue obtained during keratoplasty procedures. A 5-0 prolene suture was passed from the Bowman's layer toward the endothelial side to guide ring insertion through a narrow stromal tunnel. Intraoperative challenges, including suture disengagement and tunnel-related issues, were documented and analyzed. Twenty-three eyes of 23 patients were included. Combined CAIRS and collagen cross-linking (CXL) were performed in 78.3% of patients. The median ring width was 1.5 mm, and femtosecond-created tunnels had a median inner diameter of 4.8 mm and outer diameter of 7.8 mm. Intraoperative complications occurred in 4 cases (17.4%), all due to prolene suture disengagement. No statistically significant association was found between these events and surgical parameters. Re-passing the suture from the Bowman's side reliably restored secure guidance and enabled successful implantation in all cases. In all cases, the surgery was successfully completed under prolene suture guidance. CAIRS is a minimally invasive and customizable treatment option for corneal ectasia. The prolene suture-guided technique described in this study may facilitate graft implantation, which is one of the most technically challenging steps of the procedure, by enabling the placement of relatively wide allogenic segments into stromal tunnels without the need for graft dehydration. This approach appears to be a practical and reproducible refinement in CAIRS surgery.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148027532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematomas in musculoskeletal tissue donation: do they really increase microbiological risk?","authors":"Rossella Ravaglia, Andrea Della Valle, Francesco Puglia, Jacopo Menini, Alessandra Menon, Riccardo Compagnoni, Pietro Simone Randelli","doi":"10.1007/s10561-026-10224-4","DOIUrl":"10.1007/s10561-026-10224-4","url":null,"abstract":"<p><p>Hematoma presence in musculoskeletal tissues procured from cadaveric donors is traditionally considered a potential risk factor for microbiological contamination. This perception has led to the frequent exclusion of hematoma-bearing tissues from allograft use, despite limited scientific evidence supporting this practice. We conducted a prospective case-control study on 234 musculoskeletal tissues retrieved from 16 cadaveric donors between September 2023 and December 2024. For each tissue, data on hematoma presence and microbiological culture results were collected. Fisher's exact test was used to assess the association between hematoma presence and microbiological positivity. Odds ratios (OR) and 95% confidence intervals (CI) were calculated. A secondary intra-donor analysis examined concordance of microbiological results in paired bilateral tissues. Hematomas were observed in 25 tissues (10.7%). Overall microbiological positivity was 5.1% (12/234), with 8.0% (2/25) in tissues with hematoma and 4.8% (10/209) in tissues without hematoma. The difference was not statistically significant (OR 1.73; 95% CI: 0.35-8.48; p = 0.62). No bilateral concordance of positivity was observed in paired tissues, and unilateral positivity was found only in tissues without hematoma. Hematoma presence in musculoskeletal tissues from cadaveric donors does not appear to significantly increase microbiological contamination risk. These findings suggest that hematoma alone should not be considered an automatic exclusion criterion. Larger studies are needed to confirm these results and guide evidence-based updates to tissue bank policies.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13212684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148027537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contamination rates in umbilical cord Mesenchymal stromal cell cryopreservation: a report from Vinmec tissue bank.","authors":"Thanh-Truc Nguyen, Chi Quynh Nguyen, Tu Dac Nguyen, Tinh Van Nguyen, Kim-Chi Thi Tran, Xuan-Hung Nguyen, Uyen Thi Trang Than","doi":"10.1007/s10561-026-10221-7","DOIUrl":"10.1007/s10561-026-10221-7","url":null,"abstract":"<p><p>Stromal cells hold immense promise for advancing scientific research and medical innovation. The establishment of a biobank for stem cells, specifically umbilical cord (UC)-derived mesenchymal stromal cells (UCMSCs), is crucial for advancing medical research and regenerative therapies. A biobank provides a centralized and standardized repository of high-quality biological materials, which is essential for researchers and clinicians to explore disease mechanisms, test therapeutic approaches, and develop precision treatments. This study applied microbial testing protocols at Vinmec Hospital, including aerobic and anaerobic culture systems (BacT/ALERT® FA Plus and FN Plus), followed by bacterial identification with the VITEK® 2 Compact system, to ensure contamination-free UCMSC samples suitable for banking. A total of 1,336 UC samples were monitored at three check-points (CPs), including CP1 (UC transfer solution during collection), CP2 (cell washing buffer during isolation), and CP3 (cell culture media at P0 and P1 during expansion). Results showed that 29 cases (2.17 %) were contaminated at CP1, and only four cases (0.299 %) at CP2 showed contamination, which were identified as Candida albicans, Staphylococcus epidermidis, Escherichia coli, and Listeria monocytogenes, and were discarded. No contamination occurred at CP3, ensuring effective control during expansion. These results suggest that contamination primarily occurred during the UC collection and processing stages, while the culture and expansion procedures were not a significant source of microbial risk. Additionally, the careful implementation of these methodologies demonstrates the feasibility of generating a reliable UCMSC biobank, a cornerstone resource for personalized therapies, disease modeling, and drug discovery.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13102930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determinants of awareness and attitudes toward umbilical cord blood donation and banking among pregnant women: the role of socioeconomic and religious factors.","authors":"Sebahat Kuşlu, Beyhan Şimşek, Simge Zeyneloğlu, Hatice Serap Koçak, Nurten Doğan","doi":"10.1007/s10561-026-10223-5","DOIUrl":"10.1007/s10561-026-10223-5","url":null,"abstract":"<p><p>This study assessed pregnant women's awareness and attitudes toward umbilical cord blood donation and banking, and identified independent factors associated with awareness. This study was conducted in Gaziantep Province, Turkey, between March and June 2025, using a descriptive, cross-sectional design involving 204 pregnant women. Participants were recruited using a non-probability convenience sampling method. Data were collected via an online questionnaire that assessed socio-demographic characteristics and knowledge and attitudes towards umbilical cord blood donation and banking. Awareness levels were categorized as \"no knowledge\", \"limited knowledge\", and \"sufficient knowledge\", and dichotomized as \"insufficient\" versus \"partial/sufficient\" for regression analysis. Descriptive statistics and the Chi-square test were used to analyze the data. In addition to descriptive statistics, binary logistic regression analysis was performed to identify independent factors associated with awareness of umbilical cord blood donation and banking. Statistical significance was set at p < 0.05. The average age of the pregnant women was 30.38 ± 4.48. Only 3.9% had sufficient knowledge about UCB donation and banking, while the majority (96.1%) had never received any information about UCB donation, and none had ever donated before. The main reason for not donating was a lack of information (89.7%). The income and education level of the pregnant women were significantly associated with their knowledge and attitudes towards UCB donation and banking (p < 0.05). A family history of disease positively influenced knowledge and attitudes (p < 0.05), whereas religious beliefs negatively influenced donation intentions. Again, most pregnant women (79.9%) stated that they wanted to receive education about UCB donation and banking. Binary logistic regression analysis identified education level as the strongest independent predictor of awareness. In light of these findings, education on UCB donation and banking should be included in prenatal care processes. Involving women, families, and communities in awareness-raising initiatives can increase knowledge and participation in donation programmes.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of post-mortem time and temperature on microbiological safety, structural integrity, and biomaterial properties of human peroneus longus tendons.","authors":"Shangping Wang, Peng Chen, Dustin Mueller, R Glenn Hepfer, Haiyang Ma, Brooke Damon, Lia Campbell, Yongren Wu, Alyce Linthurst Jones, Kelvin G M Brockbank, Hai Yao","doi":"10.1007/s10561-026-10222-6","DOIUrl":"10.1007/s10561-026-10222-6","url":null,"abstract":"<p><p>Current tendon procurement practices follow the American Association of Tissue Banks (AATB) standards (15th Edition). With increasing demand for tendon allografts in the United States, extending the post-mortem interval (PMI) could enhance tissue availability for transplantation. This study evaluates the effects of extending PMIs up to 36 h on microbiological safety, tissue structure, and biomaterial properties of human peroneus longus (PL) tendon tissues, compared to current AATB standards. Our findings indicate that extending PMIs does not increase contamination risk, maintaining microbiological acceptability under extended warm ischemic conditions. Fixed charge density (FCD), an indicator of glycosaminoglycan (GAG) content, remained stable under extended PMIs and temperatures. Sirus red staining revealed a significant reduction in collagen intensity in warm tendons with extended PMIs, whereas cooling effectively preserved collagen content for up to 12 h beyond the standard. This collagen reduction may contribute to a disorganized matrix structure, facilitating increased water infiltration and greater porosity in the warm groups compared to the cooled groups. As microbiological, biomaterial and mechanical integrity remained uncompromised, our results suggest that extending PMI by up to 12 h, particularly under cooled conditions, is a viable approach to expanding the tendon donor pool for transplantation.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of reduced graphene oxide nanoparticles with linalyl acetate and lavender extract nanoemulsion on neural marker expression in human WJ-MSCs.","authors":"Mohsen Ghiasi, Fatemeh Kheirollahzadeh, Ali Zarei, Arash Padash, Hossein Dehghan, Marzieh Ghollasi","doi":"10.1007/s10561-026-10217-3","DOIUrl":"10.1007/s10561-026-10217-3","url":null,"abstract":"<p><p>Wharton's jelly-derived human mesenchymal stem cells (WJ-MSCs) have received much attention in recent years due to their non-invasive isolation method and potential for allogeneic transplantation. This study assessed the differentiation of human WJ-MSCs into neuron-like cells using retinoic acid (RA), 3-isobutyl-1-methylxanthine (IBMX), in combination with reduced graphene oxide nanoparticles (rGO), linalyl acetate (LA), and lavender extract nanoemulsion (LEN). Lavender extract was obtained through sequential extraction using n-hexane, methanol, and ethyl acetate, followed by nanoemulsion preparation. The nanoemulsion properties were analyzed by dynamic light scattering (DLS), zeta potential measurement, and transmission electron microscopy (TEM). Optimal doses were determined via MTT assay for cell viability and Acridine Orange/Ethidium Bromide (AO/EB) staining for cell death detection. The expression of neuron-specific genes (NSE, MAP-2, β-tubulin III, and Oligo-2) was analyzed using quantitative Real-time PCR (qPCR). Furthermore, the protein levels of neuronal markers gamma-enolase (NSE), MAP-2, and β-tubulin III were assessed by immunocytochemistry (ICC) on days 7 and 14. Gene and protein expression analyses demonstrated that rGO-LEN and rGO-LA significantly (p-value ≤ 0.05) enhance the neural differentiation of human WJ-MSCs. These treatments induced a significant increase in the expression of neural markers (both at the gene and protein levels) on day 7 and especially on day 14 compared to the control group and other treatments. These findings can provide initial insights to guide and develop future research to achieve new and effective therapeutic approaches in the field of regenerative medicine and neuroregenerative research.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Optimizing a feasible protocol for acellular nerve allografts: An experimental study.","authors":"Marta de Juan Marín, Marta Pevida, Sara Llames, Juan Argüelles Luís, Daniel Camporro Fernández, Álvaro Meana","doi":"10.1007/s10561-026-10216-4","DOIUrl":"10.1007/s10561-026-10216-4","url":null,"abstract":"","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13035866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioglass nanofibers in a 3D scaffold orchestrate robust bone regeneration via enhanced osteogenesis and mineralization.","authors":"Nasrin Kheradmand, Morteza Alizadeh, Tayebe Artimani, Lobat Tayebi, Mehdi Azizi, Hadi Samadian","doi":"10.1007/s10561-026-10218-2","DOIUrl":"10.1007/s10561-026-10218-2","url":null,"abstract":"<p><p>The main aim of the present study is to fabricate a 3D scaffold loaded with bioglass nanofibers to orchestrate robust bone regeneration via enhanced osteogenesis and mineralization. We applied a sol-gel method to fabricate the bioglass nanofibers and incorporated them into 3-D hydrogel (1%, 5%, and 10% wt.%). The fabricated scaffolds were characterized to determine their morphology, surface functional groups, porosity, swelling capacity, biodegradation profile, cytocompatibility, and hemocompatibility. Finally, the scaffolds were implanted in critical-size bone-defect models in the rabbit calvaria. The results showed that the calcination of precursor nanofibers to fabricate bioglass nanofibers reduced the nanofibers' diameter from around 981 nm to around 566 nm. Hydrogels containing 0%, 1%, and 5% BGNFs demonstrated no cytotoxicity, with viability percentages of 113%, 105.7%, and 112%, respectively. The 10% BGNF formulation exhibited a minor decrease in vitality (84.3%). Notably, the scaffolds promoted osteogenesis to a high degree, as evidenced by a concentration-dependent increase in mineral deposition under Alizarin Red staining. The animal studies showed that scaffold implantation supported new bone formation, as evidenced by histological analysis and micro-CT imaging. A denser, more interconnected bony network with superior mineralization (BMD) was the result of a remarkable rise in bone volume (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N), as well as a decrease in trabecular separation (Tb.Sp). The most significant outcomes were achieved when stem cells were combined with the BGNF scaffold. In summary, these results demonstrate that the bioactive nanocomposite scaffold is an excellent starting point for functional bone repair.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}