Cell and Tissue Banking最新文献_第4页

Jordanian nursing students' knowledge and attitudes of corneal donation. 约旦护理专业学生对角膜捐献的认识和态度。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-26 DOI: 10.1007/s10561-024-10145-0

Sanaa Rawashdeh, Mohammed ALBashtawy, Salam Bani Hani, Bayan ALBashtawy, Ahmad Ayed, Abdullah Alkhawaldeh, Asem Abdalrahim, Haitham Khatatbeh, Mohammad Suliman, Sa'd ALBashtawy, Islam Ali Oweidat, Rasmieh Al-Amer, Zaid ALBashtawy

{"title":"Jordanian nursing students' knowledge and attitudes of corneal donation.","authors":"Sanaa Rawashdeh, Mohammed ALBashtawy, Salam Bani Hani, Bayan ALBashtawy, Ahmad Ayed, Abdullah Alkhawaldeh, Asem Abdalrahim, Haitham Khatatbeh, Mohammad Suliman, Sa'd ALBashtawy, Islam Ali Oweidat, Rasmieh Al-Amer, Zaid ALBashtawy","doi":"10.1007/s10561-024-10145-0","DOIUrl":"10.1007/s10561-024-10145-0","url":null,"abstract":"A high success rate of corneal transplants is evident. However, there is still a lack of corneal grafts available to meet demand, largely because donors are reluctant to donate. Given their critical role in future healthcare teaching and advocacy. There has not been much research on Jordanian nursing students' perspectives on corneal donation, so it's critical to identify and eliminate any obstacles. This study aims to evaluate the knowledge and attitudes of Jordanian nursing students concerning corneal donation. A cross-sectional, descriptive design was used to recruit (n = 440) nursing students from four Jordanian universities. A self-reported questionnaire was used to obtain data on knowledge and attitudes regarding corneal donation. The average age of senior nursing students was (M = 23.07, SD = 3.63) years. Varying levels of understanding were revealed amongst university students toward corneal donation items. Generally, good attitude of nursing students toward corneal donation (M = 34.1, SD = 8.1). Weak positive relationship was found between total knowledge scores and age (r = 0.141, p = 0.003) while there is no significant relationship between age and total attitude score (r = 0.031, p = 0.552). Age was found to be a significant predictor (B = 0.01, Beta = 0.12, t = 2.07, p = 0.04). Also, the educational level of fathers is a significant positive predictor (Beta = 0.128, p = 0.008) for the total attitude scores among nursing students. Limited awareness of corneal donation, highlighting the need for focused educational interventions to improve their comprehension.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"861-872"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freezing does not influence the microarchitectural parameters of the microstructure of the freshly harvested femoral head bone. 冷冻不会影响新采集股骨头的微观结构参数。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-08-05 DOI: 10.1007/s10561-024-10147-y

Virginie Taillebot, Théo Krieger, Aurélien Maurel-Pantel, Youngji Kim, Matthieu Ollivier, Martine Pithioux

{"title":"Freezing does not influence the microarchitectural parameters of the microstructure of the freshly harvested femoral head bone.","authors":"Virginie Taillebot, Théo Krieger, Aurélien Maurel-Pantel, Youngji Kim, Matthieu Ollivier, Martine Pithioux","doi":"10.1007/s10561-024-10147-y","DOIUrl":"10.1007/s10561-024-10147-y","url":null,"abstract":"The femoral head is one of the most commonly used bones for allografts and biomechanical studies. However, there are few reports on the trabecular bone microarchitectural parameters of freshly harvested trabecular bones. To our knowledge, this is the first study to characterize the microstructure of femoral heads tested immediately after surgery and compare it with the microstructure obtained with conventional freezing. This study aims to investigate whether freezing at -80 °C for 6 weeks affects the trabecular microstructure of freshly harvested bone tissue. This study was divided into two groups: one with freshly harvested human femoral heads and the other with the same human femoral heads frozen at -80 °C for 6 weeks. Each femoral head was scanned using an X-ray microcomputed tomography scanner (µCT) to obtain the microarchitectural parameters, including the bone volume fraction (BV/TV), the mean trabecular thickness (Tb.th), the trabecular separation (Tb.sp), the degree of anisotropy (DA), and the connectivity density (Conn.D). There was no statistically significant difference between the fresh and the frozen groups for any of the parameters measured. This study shows that freezing at -80 °C for 6 weeks does not alter bone microstructure compared with freshly harvested femoral heads tested immediately after surgery.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"747-754"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141892988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical evaluation of a sheep tracheal scaffold. 绵羊气管支架的生物力学评估。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI: 10.1007/s10561-024-10130-7

Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi

{"title":"Biomechanical evaluation of a sheep tracheal scaffold.","authors":"Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi","doi":"10.1007/s10561-024-10130-7","DOIUrl":"10.1007/s10561-024-10130-7","url":null,"abstract":"Tissue engineering is a set of techniques for producing or reconstructing tissue that primarily aims to restore or improve the function of tissues in the human body. The aim of the present study was to evaluate the mechanical and histological characteristics of decellularized tracheal scaffolds prepared in comparison with fresh trachea for use in tracheal repair. In order to prepare the scaffold, sheep's trachea was prepared and after cleaning the waste tissues, they were decellularized. Then decellularized scaffolds were evaluated histologically and laboratory and numerical study of the nonlinear mechanical behavior of tracheal tissue and scaffold and their comparison. Examining the results of histological evaluations showed that the decellularization of the scaffolds was completely done. These results were confirmed by hematoxylin-eosin staining. Also, the exact hyperelastic properties of tracheal tissue and scaffold were used in biomechanical models, and according to the presented results, the five-term Mooney-Rivlin strain energy density function became a suitable behavioral model for modeling the hyperelastic behavior of trachea and scaffold. In total, the results of this research showed that the scaffolds obtained from decellularization by preserving the main compositions of the desired tissue can be a suitable platform for investigating cell behaviors.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"765-772"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141075582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of Quercetin on wound healing in the human umbilical vein endothelial cells. 槲皮素对人脐带静脉内皮细胞伤口愈合的影响

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-28 DOI: 10.1007/s10561-024-10144-1

Bahar Kartal, Ebru Alimogullari, Pınar Elçi, Tugba Fatsa, Sema Ören

{"title":"The effects of Quercetin on wound healing in the human umbilical vein endothelial cells.","authors":"Bahar Kartal, Ebru Alimogullari, Pınar Elçi, Tugba Fatsa, Sema Ören","doi":"10.1007/s10561-024-10144-1","DOIUrl":"10.1007/s10561-024-10144-1","url":null,"abstract":"An injury that affects the integrity of the skin, either inside or externally, is called a wound. Damaged tissue is repaired by a set of cellular and molecular mechanisms known as wound healing. Quercetin, a naturally occurring flavonoid, may hasten the healing of wounds. The study's objective was to investigate any potential impacts of quercetin on the wound-healing process. Human umbilical vein endothelial cells (HUVECs) were treated to varying dose ranges of quercetin (5-320 nM) for 24 and 48 h. Cultured cells were evaluated by using the MTT analysis, wound scratch assay and vascular tube formation. Furthermore the gene expression of VEGF and FGF were evaluated by qRT-PCR to determine the effects of quercetin on angiogenezis and wound repair. Positive effects of quercetin on cellular viability were demonstrated by the MTT experiment. In HUVECs quercetin promoted tube formation, migration, and proliferation while also averting wound breakage. Moreover, quercetin increased the expression of the FGF and VEGF genes, which aid in the healing of wounds in HUVECs. Quercetin may be bioactive molecule that successfully speeds up wound healing by regulating the vasculogenezis and healing cells.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"851-860"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing a bone bank within a hospital setting in India: early insights from a tertiary care center in Northern India-a review article. 在印度医院环境中建立骨库：印度北部一家三级医疗中心的早期启示--综述文章。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-07-16 DOI: 10.1007/s10561-024-10146-z

Anil Regmi, Bishwa Bandhu Niraula, Vikas Maheshwari, Hawaibam Nongdamba, Rahul Karn, Parshwanath Bondarde, Utsav Anand, Mohit Dhingra, Pankaj Kandwal

引用次数: 0

Comparison of the structural integrity and quality of corneal endothelium stored in organ culture storage medium versus Eusol-C. 比较器官培养储存介质与 Eusol-C 中储存的角膜内皮的结构完整性和质量。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-31 DOI: 10.1007/s10561-024-10138-z

Qin Wang, Yonghuan Liu, Yang Gao, Ming Zhang, Lihong Yang, Jian Hu, Yi Ren, Qizhi Zhou, Meidong Zhu

{"title":"Comparison of the structural integrity and quality of corneal endothelium stored in organ culture storage medium versus Eusol-C.","authors":"Qin Wang, Yonghuan Liu, Yang Gao, Ming Zhang, Lihong Yang, Jian Hu, Yi Ren, Qizhi Zhou, Meidong Zhu","doi":"10.1007/s10561-024-10138-z","DOIUrl":"10.1007/s10561-024-10138-z","url":null,"abstract":"In this experimental study, we compared the structural integrity and cell quality of corneal endothelium stored in organ culture medium (OCS) and Eusol-C. The experiment included rabbit and human cornea experiments in vitro. Thirty rabbit corneas and thirty-two human corneas were collected and divided into two groups. All right corneas were allocated in experiment group and left corneas were placed in control group. The corneas in experimental group were stored in OCS at 34 °C, and the corneas in control group were stored in Eusol-C at 4 °C for 7, 14, 21, 28, and 35 days, respectively. Endothelial cell morphology, cell count, and trypan blue staining for viability were assessed before storage (Day 0) and at days 7, 14, 21, 28 and 35. The structural integrity of human corneal endothelial cell was analyzed using immunohistochemistry. The samples of storage solution for microbial culture were collected on the third day and at the end of storage. The results show that no bacterial and fungal infections were found in both groups. After 14 days of storage, the morphology of endothelial cell was better in the experimental group than in the control group. The endothelial cell stored in OCS were better than those stored in Eusol-C at the end of storage times, except human cornea 14 days storage group. The ZO-1 protein staining showed the typical polygonal morphology of endothelial cell stored in the OCS. Corneal endothelial cells stored in the OCS had better quality up to 28 days. It can be applied to Chinese eye banks as a method of corneal preservation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"839-850"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of nanographene oxide on adipose-derived stem cell cryopreservation. 纳米氧化物对脂肪干细胞冷冻的影响

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-06 DOI: 10.1007/s10561-024-10140-5

Zifei Li, Jun Qi, Su Fu, Jie Luan, Qian Wang

{"title":"Effects of nanographene oxide on adipose-derived stem cell cryopreservation.","authors":"Zifei Li, Jun Qi, Su Fu, Jie Luan, Qian Wang","doi":"10.1007/s10561-024-10140-5","DOIUrl":"10.1007/s10561-024-10140-5","url":null,"abstract":"Cryoinjury mitigation is key in cell cryopreservation. Here, we aimed to assess the effectiveness of nanographene oxide (nano-GO) for improving cryoprotectant agents (CPAs) in human adipose stem cell (hADSC) cryopreservation. For in vitro experiments, nano-GO (5 μg/mL) was added to the CPAs in the control, and passage (P) 2 hADSCs were collected and cryopreserved for around two weeks. We compared cytotoxicity, cell viability, immunophenotypes, proliferation, cell apoptosis, and tri-lineage differentiation. In vivo, studies used lipoaspirate to create non-enriched or hADSC-enriched fat tissues by combining it with PBS or hADSCs cryopreserved with the aforementioned CPAs. Each nude mouse received a 0.3 mL subcutaneous injection of the graft. At 12 weeks, the grafts were harvested. Histology, adipocyte-associated genes and protein, vascular density and angiogenic cytokines, macrophage infiltration, and inflammatory cytokines were analyzed. Nano-GO CPA contributed to increased cell viability, improved cell recovery, and lowered levels of early apoptosis. Nano GO at concentrations of 0.01-100 μg/mL caused no cytotoxicity to hADSCs. The absence of nano GOs in the intracellular compartments of the cells was confirmed by transmission electron microscopy. The fat grafts from the CPA-GO group showed more viable adipocytes and significantly increased angiogenesis compared to the PBS and CPA-C groups. Adding hADSCs from the CPA-GO group to the graft reduced macrophage infiltration and MCP-1 expression. Nano-GO plays an anti-apoptotic role in the cryopreservation of hADSCs, which could improve the survival of transplanted fat tissues, possibly via improved angiogenesis and lower inflammatory response in the transplanted adipose tissue.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"805-830"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of acellular scaffold loaded with Wharton's jelly-derived stem cells and mineral pitch on healing of burn model in rat. 含有沃顿果冻干细胞和矿物沥青的细胞支架对大鼠烧伤模型愈合的影响。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-13 DOI: 10.1007/s10561-024-10143-2

Linda Mohammadzadeh Boukani, Maryam Ezzati, Ahad Ferdowsi Khosroshahi, Raziyeh Kheirjou

{"title":"The effect of acellular scaffold loaded with Wharton's jelly-derived stem cells and mineral pitch on healing of burn model in rat.","authors":"Linda Mohammadzadeh Boukani, Maryam Ezzati, Ahad Ferdowsi Khosroshahi, Raziyeh Kheirjou","doi":"10.1007/s10561-024-10143-2","DOIUrl":"10.1007/s10561-024-10143-2","url":null,"abstract":"Severe burns often result in an exacerbated inflammatory response, which can contribute to further injury. This inflammatory response may lead to an increased risk of infection, multiple organ failure, and death. This study aimed to investigate the potential of reducing inflammation to enhance burn wound healing in rats using ovine's small intestinal submucosa as a carrier for Wharton's jelly mesenchymal stem cells (WJ-MSCs) and Mineral Pitch (MP). A rat burn model was developed, and the animals were divided into four groups: control group: burn, placebo group: scaffold-treated burn, cell experimental group: WJ-MSCs seeded scaffold-treated burn, and cell and MP experimental group: scaffolds loaded with WJ-MSCs and MP-treated burn. After treating the wounds in the relevant groups and sampling them on days 5, 14 and 21, histological and pathological parameters, and the expression of genes involved in angiogenesis and epithelialization were evaluated. The study results revealed several findings in the burn wounds. These included changes in mast cell populations, a decrease in inflammatory neutrophils and lymphocytes, an increase in fibroblasts and blood vessels, and upregulation of angiogenesis and epithelialization genes. These changes collectively contributed to enhanced wound healing in cell and MP experimental group compared to the other groups. The findings suggest that scaffolds loaded with Wharton's jelly-derived stem cells and MP can serve as engineered tools to modulate inflammatory conditions during the burn wound healing process. These interventions can improve burn wound management and promote better outcomes.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"785-804"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Background, establishment and initial experiences of the Danish cardiovascular homograft biobank 丹麦心血管同种移植生物库的背景、建立和初步经验

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-07-27 DOI: 10.1007/s10561-024-10137-0

Margrete Stenehjem, Dorte Kinggaard Holm, Lars Riber, Christian Nielsen, Sara Schødt Riber, Cengiz Akgül, Jes S. Lindholt

{"title":"Background, establishment and initial experiences of the Danish cardiovascular homograft biobank","authors":"Margrete Stenehjem, Dorte Kinggaard Holm, Lars Riber, Christian Nielsen, Sara Schødt Riber, Cengiz Akgül, Jes S. Lindholt","doi":"10.1007/s10561-024-10137-0","DOIUrl":"https://doi.org/10.1007/s10561-024-10137-0","url":null,"abstract":"Odense University Hospital is a major tertiary vascular hospital in Scandinavia, performing approx. 200 aortic repairs annually. This article presents the rationale behind this endeavor and the early outcomes of the initial implantation of locally processed homografts. All patients receiving a homograft were identified from the established homograft biobank database and their medical records were reviewed after obtaining consent. All surgeons in charge of homograft implantations were semi structured interviewed regarding the harvesting procedure, the tools for detecting available homografts, their quality and delivery. The National board of Health approved the biobank fulling the EU Directive of Tissues and Cells after 18 months of preparation. From May 6, 2021, to March 1, 2023, 26 patients had a homograft implantation, with 7 for mycotic aneurysms, 10 for aorto-iliac graft infection, 6 for infra-inguinal graft infection, and 3 for graft infection in thoracic aorta. Six (23%) were emergently performed. Two (7.7%) died within 30 days postoperatively, both following in situ replacement of an infected aortoiliac graft, corresponding to a 20% mortality in this subgroup. The incidence of reinfections was 19.2%; one each in the mycotic aneurysm group, the aortoiliac graft infection group, and the thoracic graft infection group. After 90 days, two patients were diagnosed with aorto-enteric fistula. All involved surgeons could easily identify available suitable homografts, and within 2 h have homografts of acceptable quality and requested dimensions. The establishment of the Danish Cardiovascular Homograft Biobank was straightforward and effectively serves cardiovascular procedures performed 24/7. Additionally, the initial experiences seem comparable to others experiences.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"138 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141773073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical and histological comparison of two suture configurations for soft tissue grafts: speedtrap™ versus krackow stitch. 用于软组织移植的两种缝合配置的生物力学和组织学比较：Speedtrap™ 与 Krackow 缝合。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2024-05-10 DOI: 10.1007/s10561-024-10133-4

Anell Olivos-Meza, Edgar Manuel Hernández-Espinoza, Víctor Manuel Domínguez-Hernández, Víctor Manuel Araujo-Monsalvo, Eréndira Georgina Estrada-Villaseñor, Raúl Pichardo-Bahena, Luis Ángel Murguía-González, Juan Diego Rodríguez-Reinoso, Jonatan de Jesus Hernández-León, Carlos Landa-Solis

{"title":"Biomechanical and histological comparison of two suture configurations for soft tissue grafts: speedtrap™ versus krackow stitch.","authors":"Anell Olivos-Meza, Edgar Manuel Hernández-Espinoza, Víctor Manuel Domínguez-Hernández, Víctor Manuel Araujo-Monsalvo, Eréndira Georgina Estrada-Villaseñor, Raúl Pichardo-Bahena, Luis Ángel Murguía-González, Juan Diego Rodríguez-Reinoso, Jonatan de Jesus Hernández-León, Carlos Landa-Solis","doi":"10.1007/s10561-024-10133-4","DOIUrl":"10.1007/s10561-024-10133-4","url":null,"abstract":"To compare 2 different graft preparation techniques to determine biomechanical strength and resultant tissue trauma evaluated by histology. Twelve common flexors of the finger's tendons were prepared with either tubulization (SpeedTrap™) or transtendon stiches (Orthocord™). The stiffness, resistance and energy at maximum load were tested for biomechanical assessment in both groups. After load testing, Samples were stained with hematoxylin and eosin (HE) to evaluate histological damage. We observe that the time to prepare tendons with SpeedTrap™ was 8.3 times faster (1:25 min) than traditional ones (15:02 min). In all cases, the mean values for SpeedTrap™ were higher in terms of strength, stiffness and energy at maximum load than for traditional suture but without significant difference (p > 0.05). The Krackow stitch produces greater structural damage to the collagen fibers while SpeedTrap™ maintains better organized arrangement of the fibers after tubulization preparation. With the results obtained, we can conclude that the tubulization technique allows faster graft preparation with less structural damage to the manipulated tissue without altering the biomechanical resistance provided by the transtendon suture technique.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"705-712"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0