Cell and Tissue Banking最新文献_第4页

Role of secretomes in cell-free therapeutic strategies in regenerative medicine. 分泌物在再生医学无细胞治疗策略中的作用。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-02-01 DOI: 10.1007/s10561-023-10073-5

Aida Karimian, Seyedeh Mahdieh Khoshnazar, Tahmineh Kazemi, Asadollah Asadi, Arash Abdolmaleki

{"title":"Role of secretomes in cell-free therapeutic strategies in regenerative medicine.","authors":"Aida Karimian, Seyedeh Mahdieh Khoshnazar, Tahmineh Kazemi, Asadollah Asadi, Arash Abdolmaleki","doi":"10.1007/s10561-023-10073-5","DOIUrl":"10.1007/s10561-023-10073-5","url":null,"abstract":"After an injury, peripheral nervous system neurons have the potential to rebuild their axons by generating a complicated activation response. Signals from the damaged axon are required for this genetic transition to occur. Schwann cells (SCs) near a damaged nerve's distal stump also play a role in the local modulation of axonal programs, not only via cell-to-cell contacts but also through secreted signals (the secretome). The secretome is made up of all the proteins that the cell produces, such as cytokines, growth factors, and extracellular vesicles. The released vesicles may carry signaling proteins as well as coding and regulatory RNAs, allowing for multilayer communication. The secretome of SCs is now well understood as being critical for both orchestrating Wallerian degeneration and maintaining axonal regeneration. As a consequence, secretome has emerged as a feasible tissue regeneration alternative to cell therapy. Separate SC secretome components have been used extensively in the lab to promote peripheral nerve regeneration after injury. However, in neurological therapies, the secretome generated by mesenchymal (MSC) or other derived stem cells has been the most often used. In fact, the advantages of cell treatment have been connected to the release of bioactive chemicals and extracellular vesicles, which make up MSCs' secretome.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"411-426"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9153692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decellularized cryopreserved human pericardium: a validation study towards tissue bank practice. 脱细胞低温保存人心包：面向组织库实践的验证研究。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-01-25 DOI: 10.1007/s10561-023-10072-6

Giulia Montagner, Antonia Barbazza, Andrea Tancredi Lugas, Mara Terzini, Gianpaolo Serino, Cristina Bignardi, Matilde Cacciatore, Vladimiro L Vida, Massimo A Padalino, Diletta Trojan

{"title":"Decellularized cryopreserved human pericardium: a validation study towards tissue bank practice.","authors":"Giulia Montagner, Antonia Barbazza, Andrea Tancredi Lugas, Mara Terzini, Gianpaolo Serino, Cristina Bignardi, Matilde Cacciatore, Vladimiro L Vida, Massimo A Padalino, Diletta Trojan","doi":"10.1007/s10561-023-10072-6","DOIUrl":"10.1007/s10561-023-10072-6","url":null,"abstract":"Pericardial patches are currently used as reconstructive material in cardiac surgery for surgical treatment of cardiac septal defects. Autologous pericardial patches, either treated with glutaraldehyde or not, can be used as an alternative to synthetic materials or xenograft in congenital septal defects repair. The availability of an allogenic decellularized pericardium could reduce complication during and after surgery and could be a valid alternative. Decellularization of allogenic tissues aims at reducing the immunogenic reaction that might trigger inflammation and tissue calcification over time. The ideal graft for congenital heart disease repair should be biocompatible, mechanically resistant, non-immunogenic, and should have the ability to growth with the patients. The aim of the present study is the evaluation of the efficacy of a new decellularization protocol of homologous pericardium, even after cryopreservation. The technique has proven to be suitable as a tissue bank procedure and highly successful in the removal of cells and nucleic acids content, but also in the preservation of collagen and biomechanical properties of the human pericardium.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"401-410"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11142958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10619623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of caffeine intake and passive smoking on umbilical cord blood unit's quality parameters. 咖啡因摄入和被动吸烟对脐血单位质量参数的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-10-15 DOI: 10.1007/s10561-023-10111-2

Walid Almashaqbeh, Husam Ardah, Amal Alasmari, Anadel Hakeem, Shahad Alsaigh, Amairah Aloushan, Dunia Jawdat

{"title":"The effect of caffeine intake and passive smoking on umbilical cord blood unit's quality parameters.","authors":"Walid Almashaqbeh, Husam Ardah, Amal Alasmari, Anadel Hakeem, Shahad Alsaigh, Amairah Aloushan, Dunia Jawdat","doi":"10.1007/s10561-023-10111-2","DOIUrl":"10.1007/s10561-023-10111-2","url":null,"abstract":"Today cord blood (CB) is a valuable source of hematopoietic stem cells to treat many hematological disorders. One of the limitations of CB utilization is the reduced number of nucleated cells including stem cells. Therefore, CB banks around the world have developed strategies in an attempt to improve donor selection and the quality of the CB inventory. This study aimed to determine the impact of passive smoking and caffeine consumption on CB quality. CBs were obtained from mothers who gave birth at King Abdulaziz Medical City. All mothers gave their informed consent. Personal interviews about the mother's demographics, smoking status and exposure, and caffeine consumption executed, followed by a chart review to analyze maternal and neonatal factors. Laboratory testing was performed on all collected CB units. Using descriptive statistics, maternal and newborn factors were analyzed. T-test or Mann-Whitney U Test, as appropriate, for continuous variables analysis to study the effect of second hand smoking and coffee consumption for the primary outcome. Our study demonstrated a reduction in CB MNC, including lymphocytes, in caffeine consumers among pregnant donors, as well as a reduction in cell potency activities, including total CFU and BFU-E. The effect of passive cigarette smoking on the same cohort was insignificant. Outcome of this study will help in optimizing the quality and quantity of stem cell harvesting from CB to get the maximum benefit and such knowledge will raise the awareness among pregnant women.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"541-547"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts. 膝关节不同区域和部位关节软骨的生物力学特性：骨软骨异体移植的获得。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2024-02-06 DOI: 10.1007/s10561-024-10126-3

Yongsheng Ma, Qitai Lin, Xueding Wang, Yang Liu, Xiangyang Yu, Zhiyuan Ren, Yuanyu Zhang, Li Guo, Xiaogang Wu, Xiangyu Zhang, Pengcui Li, Wangping Duan, Xiaochun Wei

{"title":"Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts.","authors":"Yongsheng Ma, Qitai Lin, Xueding Wang, Yang Liu, Xiangyang Yu, Zhiyuan Ren, Yuanyu Zhang, Li Guo, Xiaogang Wu, Xiangyu Zhang, Pengcui Li, Wangping Duan, Xiaochun Wei","doi":"10.1007/s10561-024-10126-3","DOIUrl":"10.1007/s10561-024-10126-3","url":null,"abstract":"Osteochondral allograft (OCA) transplantation involves grafting of natural hyaline cartilage and supporting subchondral bone into the cartilage defect area to restore its biomechanical and tissue structure. However, differences in biomechanical properties and donor-host matching may impair the integration of articular cartilage (AC). This study analyzed the biomechanical properties of the AC in different regions of different sites of the knee joint and provided a novel approach to OCA transplantation. Intact stifle joints from skeletally mature pigs were collected from a local abattoir less than 8 h after slaughter. OCAs were collected from different regions of the joints. The patella and the tibial plateau were divided into medial and lateral regions, while the trochlea and femoral condyle were divided into six regions. The OCAs were analyzed and compared for Young's modulus, the compressive modulus, and cartilage thickness. Young's modulus, cartilage thickness, and compressive modulus of OCA were significantly different in different regions of the joints. A negative correlation was observed between Young's modulus and the proportion of the subchondral bone (r = - 0.4241, P < 0.0001). Cartilage thickness was positively correlated with Young's modulus (r = 0.4473, P < 0.0001) and the compressive modulus (r = 0.3678, P < 0.0001). During OCA transplantation, OCAs should be transplanted in the same regions, or at the closest possible regions to maintain consistency of the biomechanical properties and cartilage thickness of the donor and recipient, to ensure smooth integration with the surrounding tissue. A 7 mm depth achieved a higher Young's modulus, and may represent the ideal length.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"633-648"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139691322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preanalytical variables influencing the interpretation and reporting of biological tests on blood samples of living and deceased donors for human body materials. 影响活体和已故人体材料捐献者血液样本生物检测的解释和报告的分析前变量。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-08-25 DOI: 10.1007/s10561-023-10106-z

Elizaveta Padalko, Luc Colenbie, Alain Delforge, Nadine Ectors, Johan Guns, Romain Imbert, Hilde Jansens, Jean-Paul Pirnay, Marie-Pierre Rodenbach, Ivan Van Riet, Anne Vansteenbrugge, Gilbert Verbeken, Muriel Baltes, Hilde Beele

{"title":"Preanalytical variables influencing the interpretation and reporting of biological tests on blood samples of living and deceased donors for human body materials.","authors":"Elizaveta Padalko, Luc Colenbie, Alain Delforge, Nadine Ectors, Johan Guns, Romain Imbert, Hilde Jansens, Jean-Paul Pirnay, Marie-Pierre Rodenbach, Ivan Van Riet, Anne Vansteenbrugge, Gilbert Verbeken, Muriel Baltes, Hilde Beele","doi":"10.1007/s10561-023-10106-z","DOIUrl":"10.1007/s10561-023-10106-z","url":null,"abstract":"With the present paper, the Working Group on Cells, Tissues and Organs and other experts of the Superior Health Council of Belgium aimed to provide stakeholders in material of human origin with advice on critical aspects of serological and nucleic acid test (NAT) testing, to improve virological safety of cell- and tissue and organ donation. The current paper focusses on a number of preanalytical variables which can be critical for any medical biology examination: (1) sampling related variables (type of samples, collection of the samples, volume of the sample, choice of specific tubes, identification of tubes), (2) variables related to transport, storage and processing of blood samples (transport, centrifugation and haemolysis, storage before and after centrifugation, use of serum versus plasma), (3) variables related to dilution (haemodilution, pooling of samples), and (4) test dependent variables (available tests and validation). Depending on the type of donor (deceased donor (heart-beating or non-heart beating) versus living donor (allogeneic, related, autologous), and the type of donated human material (cells, tissue or organs) additional factors can play a role: pre- and post-mortem sampling, conditions of sampling (e.g. morgue), haemodilution, possibility of retesting.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"509-520"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10065489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activated platelet-rich plasma accelerate endometrial regeneration and improve pregnancy outcomes in murine model of disturbed endometrium. 激活的富血小板血浆可加速子宫内膜再生，改善子宫内膜紊乱小鼠模型的妊娠结局。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-07-19 DOI: 10.1007/s10561-023-10101-4

Jeevitaa Kshersagar, Akshay A Kawale, Kishor Tardalkar, Mrunal N Damle, Leena R Chaudhari, Rakesh Sharma, Meghnad G Joshi

{"title":"Activated platelet-rich plasma accelerate endometrial regeneration and improve pregnancy outcomes in murine model of disturbed endometrium.","authors":"Jeevitaa Kshersagar, Akshay A Kawale, Kishor Tardalkar, Mrunal N Damle, Leena R Chaudhari, Rakesh Sharma, Meghnad G Joshi","doi":"10.1007/s10561-023-10101-4","DOIUrl":"10.1007/s10561-023-10101-4","url":null,"abstract":"Platelet Rich Plasma (PRP) contains high concentrations of growth factors, therefore, PRP activation results in their release, stimulating the process of healing and regeneration. The study was conducted to check whether activated platelet-rich plasma (aPRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced disturbed endometrium. Seventy-two female Wistar rats were randomly assigned into the control group, disturbed endometrium (DE) group and aPRP treated group. Activation of PRP was performed by adding thrombin. All the animals were sacrificed on day 1, day 3, day 6 and day 9 and samples were taken from the miduterine horn. Quantification of Cytokine and chemokine profiles of activated and non-activated PRP for CCL2, TNF- α, IL-1β, CXCL8, CXCL10, IL2, IL4, IL-6 IL-10, IL-12, IL-17A, TGF- β, IFN-γ was carried out. Functional and structural recovery of the endometrium was analyzed by hematoxylin-eosin (HE) and immunohistochemical (IHC) analyses. HE confirmed proliferated epithelial lining and stromal reconstruction with decreased fibrosis in PRP treated group compared to the DE group. Epithelial thickness in aPRP treated on day 1, day 3, day 6 and day 9 revealed an significant increase (p ≤ 0.05). Significantly stronger IHC expression of alpha smooth muscle actin, Cytokeratin 18, Cytokeratin 19, Connexin-40, E-Cadherin, Claudin-1, Zona Occludin-1was found in the aPRP treated group compared to the DE group. Furthermore, aPRP treatment was associated with birth of live pups. Our results suggest that intrauterine administration of aPRP stimulated and accelerated the regeneration of endometrium in the murine model of disturbed endometrium.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"453-461"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9838199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Investigation of the mechanism of gamma irradiation effect on bovine bone. 撤回说明：伽马射线对牛骨作用机制的研究。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 DOI: 10.1007/s10561-023-10108-x

Noor Rahman, Rafiullah Khan, Tanvir Hussain, Nabi Ahmed

引用次数: 0

Development and validation of cryopreserved or freeze-dried decellularized human dermis for transplantation. 开发和验证用于移植的低温保存或冻干脱细胞人真皮。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2024-02-21 DOI: 10.1007/s10561-024-10131-6

Giulia Montagner, Antonia Barbazza, Manvi Pant, Andrea Tancredi Lugas, Gianpaolo Serino, Cristina Bignardi, Mara Terzini, Andrea Vantini, Jacopo Stefanelli, Diletta Trojan

{"title":"Development and validation of cryopreserved or freeze-dried decellularized human dermis for transplantation.","authors":"Giulia Montagner, Antonia Barbazza, Manvi Pant, Andrea Tancredi Lugas, Gianpaolo Serino, Cristina Bignardi, Mara Terzini, Andrea Vantini, Jacopo Stefanelli, Diletta Trojan","doi":"10.1007/s10561-024-10131-6","DOIUrl":"10.1007/s10561-024-10131-6","url":null,"abstract":"For decades, dermal tissue grafts have been used in various regenerative, reconstructive, and augmentative procedures across the body. To eliminate antigenicity and immunogenic response while still preserving the individual components and collective structural integrity of the extracellular matrix (ECM), dermis can be decellularized. Acellular dermal matrix (ADM) products like such are produced to accurately serve diverse clinical purposes. The aim of the present study is to evaluate the efficacy of a novel decellularization protocol of the human dermis, which eliminates residual human genetic material without compromising the biomechanical integrity and collagenous content of the tissue. Moreover, a freeze-drying protocol was validated. The results showed that though our decellularization protocol, human dermis can be decellularized obtaining a biocompatible matrix. The procedure is completely realized in GMP aseptic condition, avoiding tissue terminal sterilization.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"685-695"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allografts use in orthopedic surgery: trend change over the past 11 years from a regional tissue bank. 骨科手术中异体移植物的使用：一个地区组织库过去 11 年的趋势变化。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2024-02-22 DOI: 10.1007/s10561-024-10134-3

Andrea Della Valle, Riccardo Compagnoni, Francesco Puglia, Daniele Priano, Alessandra Menon, Lorenzo Teani, Giovanni Andrea La Maida, Tullia De Feo, Marco Sacchi, Federico Ambrogi, Alfonso Cicatelli, Pietro Simone Randelli

{"title":"Allografts use in orthopedic surgery: trend change over the past 11 years from a regional tissue bank.","authors":"Andrea Della Valle, Riccardo Compagnoni, Francesco Puglia, Daniele Priano, Alessandra Menon, Lorenzo Teani, Giovanni Andrea La Maida, Tullia De Feo, Marco Sacchi, Federico Ambrogi, Alfonso Cicatelli, Pietro Simone Randelli","doi":"10.1007/s10561-024-10134-3","DOIUrl":"10.1007/s10561-024-10134-3","url":null,"abstract":"Allografts are the second most transplanted tissue in medicine after blood and are now increasingly used for both primary and revision surgery. Allografts have the advantages of lower donor site morbidity, availability of multiple grafts, and shorter operative time. The Banks represents the bridge between Donor and Recipient and guarantees the quality and safety of the distributed allografts Given the increasing interest in these tissues, a retrospective analysis of data collected from the Regional Musculoskeletal Tissue Bank registry over an 11-year period (2009-2019) was conducted. The statistical analyses used were the Shapiro-Wilk normality test and a Poisson regression model. From January 2009 to December 2019, a total of 14,199 musculoskeletal tissues stored in the Regional Musculoskeletal Tissue Bank were provided for surgical allograft procedures. In 2009, the number of allografts performed was 925; this figure has steadily increased to 1599 in 2019. Epiphyses were taken as the reference tissue with an almost constant trend over the period, while a significant increase was denoted for extensor mechanism allograft, ligaments, tendons and long bone corticals (p < 0.001), processed bone tissues had no change in trend (p = 0.841). There was also a gradual decrease in the rate of microbiological positivity, as determined by bacteriological and serological tests performed on the collected tissues. This phenomenon is due to improved sampling techniques and the training of a dedicated team. Thus, we have seen how the use of allografts in orthopedic surgery has increased over the past 11 years, uniformly in terms of tissue type, except for the noticeable increase in ligamentous tissue.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"713-720"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-rich plasma alleviates knee arthritis in rats by inhibiting p65. 富血小板血浆通过抑制 p65 减轻大鼠膝关节炎。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-07-27 DOI: 10.1007/s10561-023-10102-3

Feng Zhuo, Xiaojing Jia, Zongru Wang, Yeyong Zhang, Xinfeng Yan

{"title":"Platelet-rich plasma alleviates knee arthritis in rats by inhibiting p65.","authors":"Feng Zhuo, Xiaojing Jia, Zongru Wang, Yeyong Zhang, Xinfeng Yan","doi":"10.1007/s10561-023-10102-3","DOIUrl":"10.1007/s10561-023-10102-3","url":null,"abstract":"Knee osteoarthritis (KOA) is a chronic joint disease characterized by the degeneration of articular cartilage. In this study, we explored the potential therapeutic effects of platelet-rich plasma (PRP) and identified molecular targets for treating KOA. A rat model of KOA was established via the Hulth method and primary knee joint chondrocytes were isolated to evaluate the effects of PRP and shRNA targeting p65 (sh-p65). ELISA was used to detect inflammatory factors, including IL-6, IL-1β, and TNF-α. HE staining, Safranin O/Fast Green staining and Masson staining were performed to evaluate the morphology of articular cartilage, followed by detection of p65, COL2A1, ACAN, MMP13, and ADAMTS5 expression. The proliferation and apoptosis of primary knee chondrocytes were detected by the CCK-8 assay and TUNEL staining, respectively. Treatment with either PRP or sh-p65 decreased IL-6, IL-1β, and TNF-α levels in the peripheral blood of KOA rats and chondrocyte culture supernatants, increased COL2A1 and ACAN levels, and decreased MMP13 and ADAMTS5 expression. Furthermore, administration of PRP or sh-p65 exerted protective effects on articular cartilage, enhanced the vitality of knee joint chondrocytes, and inhibited apoptosis. Collectively, PRP inhibited inflammation, promoted chondrocyte proliferation and cartilage matrix secretion, and induced cartilage regeneration by suppressing p65 expression; these effects allow PRP to alleviate KOA progression. P65-based targeted therapy administered in combination with PRP might be a promising strategy for treating KOA.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"463-473"},"PeriodicalIF":1.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9883321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0