Cell and Tissue Banking最新文献

Histological evaluation of decellularization of freeze dried and chemically treated indigenously prepared bovine pericardium membrane. 对冷冻干燥和化学处理的本土制备的牛心包膜脱细胞进行组织学评估。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-23 DOI: 10.1007/s10561-024-10139-y

Chander Gupt, Arundeep Kaur Lamba, Farrukh Faraz, Shruti Tandon, Archita Datta, Sachin Dhingra

{"title":"Histological evaluation of decellularization of freeze dried and chemically treated indigenously prepared bovine pericardium membrane.","authors":"Chander Gupt, Arundeep Kaur Lamba, Farrukh Faraz, Shruti Tandon, Archita Datta, Sachin Dhingra","doi":"10.1007/s10561-024-10139-y","DOIUrl":"10.1007/s10561-024-10139-y","url":null,"abstract":"Decellularization is regarded as a xenogenic antigen-reduction technique because it effectively eliminates all cellular and nuclear components while mitigating any negative impact on the composition, biological functionality, and structural integrity of the remaining extracellular matrix. This study aimed to histologically evaluate native, freeze dried and chemically decellularized bovine pericardium membrane. Also, this study focused on preservation of extracellular matrix after decellularization. Bovine pericardium membrane was decellularized by freeze thaw cycle followed by freeze drying and 1% sodium dodecyl sulphate. Unprocessed pericardium was used as control. The effectiveness of Decellularization was assessed based on the reduction of histologically visible nuclei. Decellularization by freeze thaw cycle followed by freeze drying resulted in 17.84% reduction in nuclei content and decellularization by sodium dodecyl sulphate results in 92% reduction in nuclei content compare to control group. Picrosirius red staining for freeze dried group displayed loosely organised, thin collagen bundles that exhibit reddish-yellow birefringence and sodium dodecyl sulfate group revealed dense collagen bundles that are parallelly organised and compact, exhibiting reddish-yellow birefringence and showed good structural integrity. These results suggested that the sodium do decyl sulfate showed optimal decellularization results with better extracellular matrix preservation. It may be a suitable protocol for producing a suitable scaffold for periodontal tissue regeneration.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dose-dependent effects of gamma radiation sterilization on the collagen matrix of human cortical bone allograft and its influence on fatigue crack propagation resistance. 伽马射线灭菌对人皮质骨异体移植物胶原基质的剂量依赖效应及其对抗疲劳裂纹扩展的影响

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-15 DOI: 10.1007/s10561-024-10135-2

Dylan B Crocker, Thomas M Hering, Ozan Akkus, Megan E Oest, Clare M Rimnac

{"title":"Dose-dependent effects of gamma radiation sterilization on the collagen matrix of human cortical bone allograft and its influence on fatigue crack propagation resistance.","authors":"Dylan B Crocker, Thomas M Hering, Ozan Akkus, Megan E Oest, Clare M Rimnac","doi":"10.1007/s10561-024-10135-2","DOIUrl":"10.1007/s10561-024-10135-2","url":null,"abstract":"Fatigue crack propagation resistance and high-cycle S-N fatigue life of cortical bone allograft tissue are both negatively impacted in a radiation dose-dependent manner from 0 to 25 kGy. The standard radiation sterilization dose of 25-35 kGy has been shown to induce cleavage of collagen molecules into smaller peptides and accumulation of stable crosslinks within the collagen matrix, suggesting that these mechanisms may influence radiation-induced losses in cyclic fracture resistance. The objective of this study was to determine the radiation dose-dependency of collagen chain fragmentation and crosslink accumulation within the dose range of 0-25 kGy. Previously, cortical bone compact tension specimens from two donor femoral pairs were divided into four treatment groups (0 kGy, 10 kGy, 17.5 kGy, and 25 kGy) and underwent cyclic loading fatigue crack propagation testing. Following fatigue testing, collagen was isolated from one compact tension specimen in each treatment group from both donors. Radiation-induced collagen chain fragmentation was assessed using SDS-PAGE (n = 5), and accumulation of pentosidine, pyridinoline, and non-specific advanced glycation end products were assessed using a fluorometric assay (n = 4). Collagen chain fragmentation increased progressively in a dose-dependent manner (p < 0.001). Crosslink accumulation at all radiation dose levels increased relative to the 0 kGy control but did not demonstrate dose-dependency (p < 0.001). Taken together with our previous findings on fatigue crack propagation behavior, these data suggest that while collagen crosslink accumulation may contribute to reduced notched fatigue behavior with irradiation, dose-dependent losses in fatigue crack propagation resistance are mainly influenced by radiation-induced chain fragmentation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Basic study on cryopreservation of rat calvarial osteoblasts with different cryoprotectants. 使用不同低温保护剂低温保存大鼠钙化成骨细胞的基础研究

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-07-08 DOI: 10.1007/s10561-024-10142-3

Xu Jiang, Tan Zhijian, Cao Min, Yu Rong, Tan Xinghui, Xin Gong

{"title":"Basic study on cryopreservation of rat calvarial osteoblasts with different cryoprotectants.","authors":"Xu Jiang, Tan Zhijian, Cao Min, Yu Rong, Tan Xinghui, Xin Gong","doi":"10.1007/s10561-024-10142-3","DOIUrl":"10.1007/s10561-024-10142-3","url":null,"abstract":"Cryopreservation is a method adopted for storage of autologous skulls. Herein, this current research sought to explore the effects of different cryoprotectants on the biological characteristics of rat calvarial osteoblasts after cryopreservation. Neonatal Sprague-Dawley rats were selected and their skull tissues were isolated. The skull tissues were allocated into the refrigerating-3M, refrigerating-6M, M199-3M, M199-6M, povidone iodine-3M, and povidone iodine-6M groups according to the usage of cryoprotectants and treatment time (month) and the fresh group. Osteoblasts were isolated from skull tissues in each group through digestion. The histomorphology of the skull was evaluated by H&E staining and cell morphology was observed by microscopy. The viability, proliferation, apoptosis, and osteogenic activity of osteoblasts were assessed by trypan blue staining, MTT, flow cytometry, and alkaline phosphatase (ALP) staining. The skull histomorphology and osteoblast morphology were similar between the fresh and refrigerating groups. Osteoblast viability was weakened after cryopreservation. The longer the refrigeration time, the lower the number of living cells and the higher the apoptosis rate. However, cryopreservation using different cryoprotectants did not evidently affect osteoblast proliferation and ALP activity. Different cryoprotectants show no apparent effect on the osteogenic activity of rat calvarial osteoblasts after cryopreservation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of cell therapy with adipose-derived stem cells in the treatment of acute rupture of the Achilles tendon in humans. 用脂肪来源干细胞进行细胞疗法治疗人类跟腱急性断裂的效果。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-21 DOI: 10.1007/s10561-024-10141-4

Maria Helena Costa Vieira, Laynna de Carvalho Schweich-Adami, Rodrigo Juliano Oliveira, Andréia Conceição Milan Brochado Antoniolli-Silva

引用次数: 0

Jordanian nursing students' knowledge and attitudes of corneal donation. 约旦护理专业学生对角膜捐献的认识和态度。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-26 DOI: 10.1007/s10561-024-10145-0

Sanaa Rawashdeh, Mohammed ALBashtawy, Salam Bani Hani, Bayan ALBashtawy, Ahmad Ayed, Abdullah Alkhawaldeh, Asem Abdalrahim, Haitham Khatatbeh, Mohammad Suliman, Sa'd ALBashtawy, Islam Ali Oweidat, Rasmieh Al-Amer, Zaid ALBashtawy

{"title":"Jordanian nursing students' knowledge and attitudes of corneal donation.","authors":"Sanaa Rawashdeh, Mohammed ALBashtawy, Salam Bani Hani, Bayan ALBashtawy, Ahmad Ayed, Abdullah Alkhawaldeh, Asem Abdalrahim, Haitham Khatatbeh, Mohammad Suliman, Sa'd ALBashtawy, Islam Ali Oweidat, Rasmieh Al-Amer, Zaid ALBashtawy","doi":"10.1007/s10561-024-10145-0","DOIUrl":"10.1007/s10561-024-10145-0","url":null,"abstract":"A high success rate of corneal transplants is evident. However, there is still a lack of corneal grafts available to meet demand, largely because donors are reluctant to donate. Given their critical role in future healthcare teaching and advocacy. There has not been much research on Jordanian nursing students' perspectives on corneal donation, so it's critical to identify and eliminate any obstacles. This study aims to evaluate the knowledge and attitudes of Jordanian nursing students concerning corneal donation. A cross-sectional, descriptive design was used to recruit (n = 440) nursing students from four Jordanian universities. A self-reported questionnaire was used to obtain data on knowledge and attitudes regarding corneal donation. The average age of senior nursing students was (M = 23.07, SD = 3.63) years. Varying levels of understanding were revealed amongst university students toward corneal donation items. Generally, good attitude of nursing students toward corneal donation (M = 34.1, SD = 8.1). Weak positive relationship was found between total knowledge scores and age (r = 0.141, p = 0.003) while there is no significant relationship between age and total attitude score (r = 0.031, p = 0.552). Age was found to be a significant predictor (B = 0.01, Beta = 0.12, t = 2.07, p = 0.04). Also, the educational level of fathers is a significant positive predictor (Beta = 0.128, p = 0.008) for the total attitude scores among nursing students. Limited awareness of corneal donation, highlighting the need for focused educational interventions to improve their comprehension.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freezing does not influence the microarchitectural parameters of the microstructure of the freshly harvested femoral head bone. 冷冻不会影响新采集股骨头的微观结构参数。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-08-05 DOI: 10.1007/s10561-024-10147-y

Virginie Taillebot, Théo Krieger, Aurélien Maurel-Pantel, Youngji Kim, Matthieu Ollivier, Martine Pithioux

{"title":"Freezing does not influence the microarchitectural parameters of the microstructure of the freshly harvested femoral head bone.","authors":"Virginie Taillebot, Théo Krieger, Aurélien Maurel-Pantel, Youngji Kim, Matthieu Ollivier, Martine Pithioux","doi":"10.1007/s10561-024-10147-y","DOIUrl":"10.1007/s10561-024-10147-y","url":null,"abstract":"The femoral head is one of the most commonly used bones for allografts and biomechanical studies. However, there are few reports on the trabecular bone microarchitectural parameters of freshly harvested trabecular bones. To our knowledge, this is the first study to characterize the microstructure of femoral heads tested immediately after surgery and compare it with the microstructure obtained with conventional freezing. This study aims to investigate whether freezing at -80 °C for 6 weeks affects the trabecular microstructure of freshly harvested bone tissue. This study was divided into two groups: one with freshly harvested human femoral heads and the other with the same human femoral heads frozen at -80 °C for 6 weeks. Each femoral head was scanned using an X-ray microcomputed tomography scanner (µCT) to obtain the microarchitectural parameters, including the bone volume fraction (BV/TV), the mean trabecular thickness (Tb.th), the trabecular separation (Tb.sp), the degree of anisotropy (DA), and the connectivity density (Conn.D). There was no statistically significant difference between the fresh and the frozen groups for any of the parameters measured. This study shows that freezing at -80 °C for 6 weeks does not alter bone microstructure compared with freshly harvested femoral heads tested immediately after surgery.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141892988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical evaluation of a sheep tracheal scaffold. 绵羊气管支架的生物力学评估。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI: 10.1007/s10561-024-10130-7

Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi

{"title":"Biomechanical evaluation of a sheep tracheal scaffold.","authors":"Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi","doi":"10.1007/s10561-024-10130-7","DOIUrl":"10.1007/s10561-024-10130-7","url":null,"abstract":"Tissue engineering is a set of techniques for producing or reconstructing tissue that primarily aims to restore or improve the function of tissues in the human body. The aim of the present study was to evaluate the mechanical and histological characteristics of decellularized tracheal scaffolds prepared in comparison with fresh trachea for use in tracheal repair. In order to prepare the scaffold, sheep's trachea was prepared and after cleaning the waste tissues, they were decellularized. Then decellularized scaffolds were evaluated histologically and laboratory and numerical study of the nonlinear mechanical behavior of tracheal tissue and scaffold and their comparison. Examining the results of histological evaluations showed that the decellularization of the scaffolds was completely done. These results were confirmed by hematoxylin-eosin staining. Also, the exact hyperelastic properties of tracheal tissue and scaffold were used in biomechanical models, and according to the presented results, the five-term Mooney-Rivlin strain energy density function became a suitable behavioral model for modeling the hyperelastic behavior of trachea and scaffold. In total, the results of this research showed that the scaffolds obtained from decellularization by preserving the main compositions of the desired tissue can be a suitable platform for investigating cell behaviors.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141075582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of Quercetin on wound healing in the human umbilical vein endothelial cells. 槲皮素对人脐带静脉内皮细胞伤口愈合的影响

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-28 DOI: 10.1007/s10561-024-10144-1

Bahar Kartal, Ebru Alimogullari, Pınar Elçi, Tugba Fatsa, Sema Ören

{"title":"The effects of Quercetin on wound healing in the human umbilical vein endothelial cells.","authors":"Bahar Kartal, Ebru Alimogullari, Pınar Elçi, Tugba Fatsa, Sema Ören","doi":"10.1007/s10561-024-10144-1","DOIUrl":"10.1007/s10561-024-10144-1","url":null,"abstract":"An injury that affects the integrity of the skin, either inside or externally, is called a wound. Damaged tissue is repaired by a set of cellular and molecular mechanisms known as wound healing. Quercetin, a naturally occurring flavonoid, may hasten the healing of wounds. The study's objective was to investigate any potential impacts of quercetin on the wound-healing process. Human umbilical vein endothelial cells (HUVECs) were treated to varying dose ranges of quercetin (5-320 nM) for 24 and 48 h. Cultured cells were evaluated by using the MTT analysis, wound scratch assay and vascular tube formation. Furthermore the gene expression of VEGF and FGF were evaluated by qRT-PCR to determine the effects of quercetin on angiogenezis and wound repair. Positive effects of quercetin on cellular viability were demonstrated by the MTT experiment. In HUVECs quercetin promoted tube formation, migration, and proliferation while also averting wound breakage. Moreover, quercetin increased the expression of the FGF and VEGF genes, which aid in the healing of wounds in HUVECs. Quercetin may be bioactive molecule that successfully speeds up wound healing by regulating the vasculogenezis and healing cells.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing a bone bank within a hospital setting in India: early insights from a tertiary care center in Northern India-a review article. 在印度医院环境中建立骨库：印度北部一家三级医疗中心的早期启示--综述文章。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-07-16 DOI: 10.1007/s10561-024-10146-z

Anil Regmi, Bishwa Bandhu Niraula, Vikas Maheshwari, Hawaibam Nongdamba, Rahul Karn, Parshwanath Bondarde, Utsav Anand, Mohit Dhingra, Pankaj Kandwal

引用次数: 0

Comparison of the structural integrity and quality of corneal endothelium stored in organ culture storage medium versus Eusol-C. 比较器官培养储存介质与 Eusol-C 中储存的角膜内皮的结构完整性和质量。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-31 DOI: 10.1007/s10561-024-10138-z

Qin Wang, Yonghuan Liu, Yang Gao, Ming Zhang, Lihong Yang, Jian Hu, Yi Ren, Qizhi Zhou, Meidong Zhu

{"title":"Comparison of the structural integrity and quality of corneal endothelium stored in organ culture storage medium versus Eusol-C.","authors":"Qin Wang, Yonghuan Liu, Yang Gao, Ming Zhang, Lihong Yang, Jian Hu, Yi Ren, Qizhi Zhou, Meidong Zhu","doi":"10.1007/s10561-024-10138-z","DOIUrl":"10.1007/s10561-024-10138-z","url":null,"abstract":"In this experimental study, we compared the structural integrity and cell quality of corneal endothelium stored in organ culture medium (OCS) and Eusol-C. The experiment included rabbit and human cornea experiments in vitro. Thirty rabbit corneas and thirty-two human corneas were collected and divided into two groups. All right corneas were allocated in experiment group and left corneas were placed in control group. The corneas in experimental group were stored in OCS at 34 °C, and the corneas in control group were stored in Eusol-C at 4 °C for 7, 14, 21, 28, and 35 days, respectively. Endothelial cell morphology, cell count, and trypan blue staining for viability were assessed before storage (Day 0) and at days 7, 14, 21, 28 and 35. The structural integrity of human corneal endothelial cell was analyzed using immunohistochemistry. The samples of storage solution for microbial culture were collected on the third day and at the end of storage. The results show that no bacterial and fungal infections were found in both groups. After 14 days of storage, the morphology of endothelial cell was better in the experimental group than in the control group. The endothelial cell stored in OCS were better than those stored in Eusol-C at the end of storage times, except human cornea 14 days storage group. The ZO-1 protein staining showed the typical polygonal morphology of endothelial cell stored in the OCS. Corneal endothelial cells stored in the OCS had better quality up to 28 days. It can be applied to Chinese eye banks as a method of corneal preservation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0