Cell and Tissue Banking最新文献

{"title":"20-year analysis of protocol-driven quality improvement in cardiac homograft banking.","authors":"Muhammad Ashraff Baruji, Sharifah Noraida Syed Nong, Khairul Anuar Abdul Aziz, Mohd Nazeri Nordin, Mohamed Ezani Md Taib, Intan Fariza Gaaffar, Aini Syakirin Kepli, Ahmad Sallehuddin","doi":"10.1007/s10561-026-10258-8","DOIUrl":"https://doi.org/10.1007/s10561-026-10258-8","url":null,"abstract":"<p><p>Cardiac homografts are indispensable in complex cardiac surgery, particularly in the paediatric population. Optimising procurement and minimising discard rates are important for maintaining graft availability, especially in resource-limited settings. This study evaluated 20 years of cardiac homograft procurement at a tertiary cardiovascular centre, focusing on discard rates, causes of discard, microbiological profiles, and the impact of protocol refinements. A retrospective analysis was conducted using procurement records from the Cardiac Homograft Tissue Bank at the Institut Jantung Negara, Malaysia, between January 2004 and December 2024. The data collected included annual procurement volume, reasons for discarding, and microbiological culture results. In 2015, a revised procurement protocol was introduced, which incorporated instrument and operative field segregation, aseptic redraping, and enhanced donor screening. A total of 190 hearts were procured (mean 9.05 ± 4.18 hearts/year), with an overall discard rate of 13.7% (of 26/190). Prior to 2015, the discard rate was 17.2% (20/116), with environmental contamination accounting for the majority of the discards (75%; n=15). Fifteen microbial isolates were identified in the pre-revision period: Candida (n=6), Klebsiella (n=4), Acinetobacter (n=2), Streptococcus spp. (n=1), Streptococcus viridans (n=1), and Pseudomonas spp. (n=1). Following the 2015 protocol revision, the overall discard rate decreased to 8.1% (6/74), representing a 53% reduction, although this difference was not statistically significant (p=0.074). Environmental contamination-related discards decreased significantly from 12.9% (15/116) to 1.4% (1/74) (89% reduction; p=0.005), with only one Candida isolate identified post-revision. Protocol refinement has therefore produced a statistically significant reduction in contamination-related discards and a shift in the microbiological spectrum.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149227398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of adipose tissue for nanofat production: biological principles, workflow standardisation, and regulated biobanking.","authors":"Ahsan A Khan, Olivier D Amar, Sumila Rathnayaka-Mudiyanselage, Sophie Menkes, Shaheel Chummun, Aurora Almadori","doi":"10.1007/s10561-026-10256-w","DOIUrl":"https://doi.org/10.1007/s10561-026-10256-w","url":null,"abstract":"<p><p>Nanofat, produced through mechanical processing of autologous adipose tissue, contains stromal vascular fraction (SVF), extracellular matrix components, and perivascular cell populations associated with paracrine, angiogenic, and immunomodulatory signalling. Cryopreservation before emulsification allows material from a single harvest to be stored and used across multiple treatments, supporting staged clinical, reconstructive, and tissue-support applications. This reduces patient burden and the morbidity of repeated liposuction, and potentially enables treatment schedules to align with tissue remodelling cycles.The cryobiology of adipose tissue and the biological properties of SVF-derived preparations are each well characterised in isolation, but the literature lacks a coherent account of how they combine in a workflow that is both clinically practical and compatible with regulatory oversight. Most published approaches describe same-day preparation, offering little structure for staged treatments without repeated harvest. This article synthesises current evidence on nanofat biology, cryobiology principles for adipose-derived preparations, mechanical processing and delivery, and governance requirements for regulated cryostorage. A generic Human Tissue Authority (HTA)-licensed cryopreservation workflow model is presented to illustrate how validated controlled-rate freezing, cryopreservation of whole lipoaspirate, thawing, and mechanical emulsification to produce nanofat, supported by traceability and a documented quality management system (QMS), can support regulated clinical use. This article addresses a gap in the literature by providing a structured pathway for consistent autologous treatments from a single harvest while meeting regulatory expectations for human tissue storage. This article is a narrative synthesis, not a systematic review; it focuses on workflow, cryobiological, and governance conditions rather than grading therapeutic efficacy.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of thermodisinfection on the mechanical properties of individual human trabeculae from femoral heads.","authors":"Katharina Biebl, Alexander Jahnke, Torben Harz, Jacob Siemssen, Sarah Szopko, Kristina Reinfandt, Markus Rickert, Christian Fölsch","doi":"10.1007/s10561-026-10254-y","DOIUrl":"https://doi.org/10.1007/s10561-026-10254-y","url":null,"abstract":"<p><p>Allogeneic bone grafts are frequently used in reconstructive orthopaedics and trauma surgery and require appropriate processing to ensure biological safety. Thermodisinfection represents an established procedure; however, its influence on the mechanical properties of individual trabeculae has not yet been sufficiently investigated. Single trabeculae were prepared from human femoral heads and assigned within a paired study design to either native or thermodisinfected conditions. Mechanical characterisation was performed using a two-point bending test. The elastic modulus (E) was determined from the linear region of the force-displacement curve, and the maximum fracture force ( <math><msub><mi>F</mi> <mtext>max</mtext></msub> </math> ) was recorded as a measure of load-bearing capacity until structural failure. Furthermore, the influence of anatomical location within the femoral head was analysed. No significant difference in elastic modulus was found between native and thermodisinfected trabeculae. The maximum fracture force was significantly increased in the thermodisinfected group. No consistent differences were observed for either parameter with respect to anatomical location within the femoral head. Thermodisinfection did not significantly impair the elastic properties of individual human trabeculae but significantly affected failure-related behaviour. No consistent influence of anatomical location was observed. These findings indicate that thermodisinfection largely preserves the intrinsic mechanical properties of individual trabeculae while also influencing failure-related behaviour. They complement previous predominantly macroscopic investigations by providing a microstructural perspective on the biomechanical effects of thermodisinfection. Furthermore, the present findings may provide a biomechanical rationale for future bone graft processing strategies involving smaller bone fragments or granule-based graft preparation, although the clinical feasibility of such applications requires further investigation.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research hotspots and emerging trends of 3D printing in bone defects: a bibliometric analysis based on Web of Science core collection from 2006 to March 2026.","authors":"Xiongjie Shen, Wenling Zhang","doi":"10.1007/s10561-026-10255-x","DOIUrl":"10.1007/s10561-026-10255-x","url":null,"abstract":"<p><p>Bone defects continue to pose a significant challenge in orthopedic and reconstructive surgery, especially when presenting as large, irregular, infected, or load-bearing lesions. The advent of three-dimensional (3D) printing has opened new avenues for customized bone repair, offering precise anatomical restoration, tunable porous architectures, mechanical support, and biological functionalization. Nevertheless, the global research landscape, intellectual framework, and emerging frontiers of 3D printing applied to bone defects have yet to be systematically characterized.Bibliometric records were retrieved from the Science Citation Index Expanded (SCI-EXPANDED) within the Web of Science Core Collection for publications from January 1, 2006, to March 15, 2026. PubMed was searched separately as a supplementary source to identify relevant clinical trials and randomized controlled trials. Bibliometric and visualization analyses were subsequently performed using VOSviewer, R Software, and CiteSpace. The analyses focused on publication output over time, country and institutional contributions, author productivity, journal impact, keyword evolution, and trends in clinical translation. A total of 7233 relevant publications were identified in this study, revealing a consistent upward trend in annual output. China and the United States were the predominant contributors in this research domain. Leading institutions included Shanghai Jiao Tong University and Sichuan University. Wu, CT emerged as the most prolific author with 62 publications, while Bose, S demonstrated the highest co-citation frequency (1,290 citations). Regarding journals, Frontiers in Bioengineering and Biotechnology recorded the largest number of publications, and Biomaterials ranked first in co-citation count. Analysis of research hotspots indicated that clinical trials primarily evaluated the efficacy of 3D-printed bone materials for the reconstruction of various bone defects. Meanwhile, basic science investigations focused on artificial intelligence, oxidative stress, antibacterial properties, macrophage polarization, and advanced scaffold functionalization. Over the past two decades, recent research attention has increasingly shifted from scaffold fabrication and material optimization toward intelligent design, immune-related research, antibacterial functionalization, and more application-specific scaffold development. Future efforts should prioritize mechanistic studies, clinically relevant defect models, multicenter trials, and problem-oriented scaffold engineering to facilitate reliable clinical translation.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149179688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of false-positive and invalid results in postmortem blood testing of tissue donors: a case-control study.","authors":"A de Graaf, M Bakker, M Koot, B Hogema, N Holsboer","doi":"10.1007/s10561-026-10248-w","DOIUrl":"https://doi.org/10.1007/s10561-026-10248-w","url":null,"abstract":"<p><p>Postmortem blood screening for infectious diseases is crucial for safe tissue transplantation. However, false-positive and invalid results occur more often than in living donors, potentially leading to unnecessary graft rejection, based on the acceptance criteria of the Dutch Transplant Foundation. This study aimed to identify factors associated with unreliable postmortem test outcomes. A matched case-control study was conducted using data from Dutch tissue donors screened between 2018 and 2023. Cases included 174 donors with false-positive or invalid results, and controls included 460 donors with reliable results. Logistic regression assessed associations between test unreliability and donor factors, including age, body mass index (BMI), sepsis, and malignancy, and procedural factors, including postmortem interval (PMI) and method of blood collection. Prolonged PMI and pathologist-collected samples with delayed processing were significantly associated with unreliable test results. Each additional hour of PMI increased the odds by 10 percent (adjusted OR 1.10, 95% CI: 1.06-1.13, p < 0.001). Blood collection prior autopsy showed over fourfold higher odds of an unreliable result (adjusted OR 4.54, 95% CI: 1.61-13.9, p = 0.005). Donor-related characteristics showed no significant associations. Unreliable postmortem test results are primarily associated with procedural factors. Reducing the PMI and standardizing sampling methods may improve test accuracy.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vancomycin elution from gamma-irradiated cancellous bone allograft: an in vitro study linking soaking concentration to clinically relevant antimicrobial thresholds.","authors":"Azura Mansor, Kah Sui Tan, Cindy Shuan Ju Teh, Zhi Xian Kong, Hai Yen Lee","doi":"10.1007/s10561-026-10257-9","DOIUrl":"https://doi.org/10.1007/s10561-026-10257-9","url":null,"abstract":"<p><p>Antibiotic-impregnated bone grafts can deliver high local antimicrobial concentrations while restoring structural defects, but wider adoption is held back by inconsistent preparation methods and elution data that are difficult to translate into intraoperative decisions. Gamma-irradiated cancellous femoral head allografts were morselised and soaked for 30 min in vancomycin solutions ranging from 0 to 100 mg/mL, using a standardised graft-to-solution ratio. Elution was assessed qualitatively by agar diffusion against Staphylococcus aureus and quantitatively by high-performance liquid chromatography (HPLC). Daily eluates were collected for up to 30 days and compared with the minimal inhibitory concentration (MIC) for S. aureus and with experimentally derived anti-biofilm reference values. A 1:1 (g:mL) graft-to-solution ratio gave mass-independent loading. Release followed a burst-then-decay profile across all concentrations. A 10 mg/mL soak sustained eluate concentrations above the S. aureus MIC for 14 days, consistent with a prophylactic use case. Soaks at 60-100 mg/mL produced Day 1 eluate concentrations of 1043-1920 mg/L, exceeding the experimental anti-biofilm reference range; these high levels were not sustained beyond the burst phase. Vancomycin elution from gamma-irradiated cancellous allograft is concentration-dependent. Pairing standardised soaking conditions with clinically relevant antimicrobial thresholds offers a practical basis for selecting graft preparation strategies-lower concentrations for prophylaxis and higher concentrations for adjunctive use after debridement. In vivo and biofilm-model validation is needed before clinical translation.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149135234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ensuring sustainable availability of frozen bone allografts: experiences and lessons from a university hospital bone bank.","authors":"Azura Mansor, Saravana Ramalingam, Suhaili Mohd, Norimah Yusof","doi":"10.1007/s10561-026-10252-0","DOIUrl":"https://doi.org/10.1007/s10561-026-10252-0","url":null,"abstract":"<p><p>University hospital bone banks are vital to tissue transplantation, providing safe bone allografts for surgical procedures. The bone bank at Universiti Malaya Medical Centre (UMMC) initially sourced femoral heads and knee slices from living donors, which were radiation-sterilized and frozen for internal orthopaedic surgeries. In 2015, through collaboration with the Ministry of Health, the bank obtained long bones from deceased donors. A 10-year retrospective analysis (2013-2022) evaluated the bank's nationwide distribution, which totalled 661 frozen bone allografts across 384 despatch batches. The supply comprised 505 femoral heads and 61 knee slices from living donors, alongside 95 deceased-donor long bones. These allografts were supplied to 57 healthcare facilities: two internal UMMC wings (public and private), 2 university hospitals, 32 government hospitals, and 22 private medical centres. To accommodate rising demand and optimize operations, the bank implemented targeted quality interventions during a remedial phase (2017-2018). Consequently, bone rejections plummeted from 50.8% during the verification phase (2013-2016) to 20.2% in the post-remedial phase (2019-2022). This significant reduction was driven by two major updates: A streamlined consent process that enhanced donor screening for surgeons, and a new bone donor kit with optimized nursing procedures improved collecting and handling bone and blood specimens. Implementing structured procedural interventions effectively mitigated tissue rejection rates and secured a steady supply of high-quality allografts. Furthermore, monitoring national transplantation data and feedback on graft quality served as an invaluable benchmark for continuous improvement in bone banking activities.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of human amniotic membrane (HAM) in tympanoplasty: a systematic review and meta-analysis.","authors":"Chamod Danosha Perera, Jharna Chhetri Chuwan, Kasun Lakmal, Dineshani Hettiarachchi","doi":"10.1007/s10561-026-10251-1","DOIUrl":"10.1007/s10561-026-10251-1","url":null,"abstract":"<p><p>Tympanic membrane perforation is a common otologic disorder. In cases where spontaneous closure does not occur, or when the perforation is associated with clinically significant symptoms that adversely affect quality of life, surgical repair is often indicated. This review and meta-analysis aimed to synthesize and critically appraise the current evidence on the use of human amniotic membrane (HAM) as a graft material in tympanoplasty. A systematic literature search was conducted in multiple databases for studies published up to November 2025. The following search terms were used in titles and abstracts (with corresponding MeSH terms applied in PubMed): \"Amnion\" OR \"Placenta\" AND \"Tympanoplasty.\" Single-arm meta-analysis using a random effects model was performed to assess improvement in the air-bone gap (ABG) and anatomical closure rates. Standardized methodological criteria were applied to assess study quality and risk of bias. Out of 630 identified records, eight studies evaluating 265 tympanoplasties (264 patients) using amniotic membrane grafting (AMG) were included. The majority of non-randomized studies carried a serious risk of bias. A single-arm meta-analysis of 4 studies demonstrated a pooled complete anatomical closure rate of 92% (95% CI 0.79-0.98; I² = 44.1%). Functional assessment via single-arm meta-analysis of 4 studies showed a statistically non-significant pooled mean difference in air-bone gap (ABG) improvement of 12.66 dB (95% CI-0.22 to 25.54), though between-study heterogeneity was high (I² = 99.4%). Operative times for AMG ranged widely across 2 reporting studies, but one comparative study noted significantly shorter times for AMG compared to temporalis fascia grafting (54.3 vs. 75.3 min). Postoperative graft failure rates were low, primarily driven by sparse instances of infection or graft lateralization. Publication bias could not be formally assessed due to the small number of included studies. HAM is a promising alternative to conventional graft material with excellent anatomical closure, improved but non-significant ABG, and lower operative times. Although current findings support HAM as a viable alternative to conventional grafts in tympanoplasty, the predominance of non-randomized studies, small sample sizes, and methodological heterogeneity constrain the strength of the conclusions. High-quality, adequately powered RCTs are required to better define the optimal indications, techniques, and long-term outcomes associated with HAM use in middle ear surgery.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osseointegration of dental implants: a systematic review and meta-analysis of evidence from preclinical animal models and human studies.","authors":"Nishita Bhosale, Vidya Dodwad, Vivek Sunil Nair, Vikrant Sane, Adhishree Joshi","doi":"10.1007/s10561-026-10250-2","DOIUrl":"10.1007/s10561-026-10250-2","url":null,"abstract":"&lt;p&gt;&lt;p&gt;The long-term success of dental implants depends on rapid and stable osseointegration. Numerous surface treatments and biologic adjuncts have been proposed to enhance early bone anchorage, but the strength and consistency of the available preclinical and clinical evidence remain uncertain. To synthesise evidence from preclinical animal models and human clinical studies evaluating the influence of titanium implant surface modifications, biologic adjuncts, and systemic factors on osseointegration-related outcomes. A protocol was established following the PRISMA 2020 guidelines and subsequently registered with PROSPERO (CRD420251153021). A comprehensive search was conducted across PubMed, Scopus, Web of Science, Cochrane Library, and Google Scholar from their inception through December 2025. The studies included in this review comprised both in vivo animal research and human clinical trials that examined modified titanium implant surfaces, supplementary biological therapies or systemic modifiers, and provided quantitative outcomes related to osseointegration. The primary outcomes measured were bone-to-implant contact percentage (BIC%), implant stability quotient (ISQ), changes in marginal bone levels, radiographic bone density, and bone volume fraction. The risk of bias was evaluated using the SYRCLE tool, Cochrane RoB 2.0, and the JBI checklist where applicable. Animal and human evidence were synthesized qualitatively, whereas random-effects meta-analysis was performed only for clinically comparable human studies reporting common outcomes. Fourteen studies met the inclusion criteria (eight animal and six human). Roughened, hydrophilic, nanostructured, UV- or plasma-activated surfaces, as well as platelet-derived concentrates and concentrated growth factors, generally demonstrated favourable effects on osseointegration-related outcomes, including higher BIC, bone volume, and earlier implant stability, whereas diabetes and pharmacologic VEGF blockade were associated with impaired osseointegration. Pooled analysis of four human trials showed higher ISQ values for test interventions (SMD 1.37; 95% CI 1.02-1.73; P &lt; 0.00001; I&lt;sup&gt;2&lt;/sup&gt; = 5%). Two human trials reporting radiographic bone density also favoured the experimental protocols (SMD -1.26; 95% CI -1.74 to -0.78; P &lt; 0.00001; I&lt;sup&gt;2 &lt;/sup&gt;= 4%). However, interpretation of the radiographic bone density analysis is limited because only two studies contributed quantitative data. Human studies generally demonstrated low-to-moderate risk of bias, whereas several animal studies showed unclear randomisation and blinding. The certainty of evidence ranged from low to moderate across the primary outcomes. The available evidence suggests that surface-optimised and biologically augmented titanium implants may improve early osseointegration and implant stability, whereas adverse systemic conditions may compromise these benefits The quality of evidence is not very good because of methodological limita","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging the gap between knowledge and barriers: evaluating healthcare professionals' perspectives on hematopoietic stem cell transplantation.","authors":"Haitham M H Qutob, Raed Alserihi","doi":"10.1007/s10561-026-10253-z","DOIUrl":"https://doi.org/10.1007/s10561-026-10253-z","url":null,"abstract":"<p><p>Hematopoietic stem cell transplantation (HSCT) is an essential treatment for various malignant and non-malignant hematological disorders. Healthcare professionals play a vital role in this process through their attitudes toward donation and their support of patients and their relatives in the decision-making process. This study was performed to examine the correlation between the knowledge and attitudes of healthcare professionals regarding HSC donation. A cross-sectional survey was conducted between September 2025 and January 2026 after ethical approval was obtained from the Council of the Faculty of Applied Medical Sciences in Rabigh at King Abdulaziz University. Data were collected via online surveys administered to medical students and healthcare professionals in both government and private tertiary hospitals. Of the 507 validated respondents, 44.8% expressed willingness to donate HSCs, whereas 55.2% indicated unwillingness. Participants willing to donate had higher knowledge scores (mean = 75.7%) and attitude scores (mean = 3.12) than those who were unwilling. A strong association was observed between higher knowledge, direct clinical exposure to HSCT, and completion of formal training (p < 0.05). The main barriers to HSC donation were time commitment, lack of knowledge, and fear of the process, with mean scores of 3.88, 3.80, and 3.79, respectively. Knowledge is an important factor but is not sufficient on its own to motivate donation. The findings indicate that direct clinical exposure and targeted educational training play a key role in fostering positive attitudes by addressing concerns related to time commitment and fear of the donation process.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}