Cell and Tissue Banking最新文献

{"title":"Contamination rates in umbilical cord Mesenchymal stromal cell cryopreservation: a report from Vinmec tissue bank.","authors":"Thanh-Truc Nguyen, Chi Quynh Nguyen, Tu Dac Nguyen, Tinh Van Nguyen, Kim-Chi Thi Tran, Xuan-Hung Nguyen, Uyen Thi Trang Than","doi":"10.1007/s10561-026-10221-7","DOIUrl":"https://doi.org/10.1007/s10561-026-10221-7","url":null,"abstract":"<p><p>Stromal cells hold immense promise for advancing scientific research and medical innovation. The establishment of a biobank for stem cells, specifically umbilical cord (UC)-derived mesenchymal stromal cells (UCMSCs), is crucial for advancing medical research and regenerative therapies. A biobank provides a centralized and standardized repository of high-quality biological materials, which is essential for researchers and clinicians to explore disease mechanisms, test therapeutic approaches, and develop precision treatments. This study applied microbial testing protocols at Vinmec Hospital, including aerobic and anaerobic culture systems (BacT/ALERT® FA Plus and FN Plus), followed by bacterial identification with the VITEK® 2 Compact system, to ensure contamination-free UCMSC samples suitable for banking. A total of 1,336 UC samples were monitored at three check-points (CPs), including CP1 (UC transfer solution during collection), CP2 (cell washing buffer during isolation), and CP3 (cell culture media at P0 and P1 during expansion). Results showed that 29 cases (2.17 %) were contaminated at CP1, and only four cases (0.299 %) at CP2 showed contamination, which were identified as Candida albicans, Staphylococcus epidermidis, Escherichia coli, and Listeria monocytogenes, and were discarded. No contamination occurred at CP3, ensuring effective control during expansion. These results suggest that contamination primarily occurred during the UC collection and processing stages, while the culture and expansion procedures were not a significant source of microbial risk. Additionally, the careful implementation of these methodologies demonstrates the feasibility of generating a reliable UCMSC biobank, a cornerstone resource for personalized therapies, disease modeling, and drug discovery.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13102930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determinants of awareness and attitudes toward umbilical cord blood donation and banking among pregnant women: the role of socioeconomic and religious factors.","authors":"Sebahat Kuşlu, Beyhan Şimşek, Simge Zeyneloğlu, Hatice Serap Koçak, Nurten Doğan","doi":"10.1007/s10561-026-10223-5","DOIUrl":"https://doi.org/10.1007/s10561-026-10223-5","url":null,"abstract":"<p><p>This study assessed pregnant women's awareness and attitudes toward umbilical cord blood donation and banking, and identified independent factors associated with awareness. This study was conducted in Gaziantep Province, Turkey, between March and June 2025, using a descriptive, cross-sectional design involving 204 pregnant women. Participants were recruited using a non-probability convenience sampling method. Data were collected via an online questionnaire that assessed socio-demographic characteristics and knowledge and attitudes towards umbilical cord blood donation and banking. Awareness levels were categorized as \"no knowledge\", \"limited knowledge\", and \"sufficient knowledge\", and dichotomized as \"insufficient\" versus \"partial/sufficient\" for regression analysis. Descriptive statistics and the Chi-square test were used to analyze the data. In addition to descriptive statistics, binary logistic regression analysis was performed to identify independent factors associated with awareness of umbilical cord blood donation and banking. Statistical significance was set at p < 0.05. The average age of the pregnant women was 30.38 ± 4.48. Only 3.9% had sufficient knowledge about UCB donation and banking, while the majority (96.1%) had never received any information about UCB donation, and none had ever donated before. The main reason for not donating was a lack of information (89.7%). The income and education level of the pregnant women were significantly associated with their knowledge and attitudes towards UCB donation and banking (p < 0.05). A family history of disease positively influenced knowledge and attitudes (p < 0.05), whereas religious beliefs negatively influenced donation intentions. Again, most pregnant women (79.9%) stated that they wanted to receive education about UCB donation and banking. Binary logistic regression analysis identified education level as the strongest independent predictor of awareness. In light of these findings, education on UCB donation and banking should be included in prenatal care processes. Involving women, families, and communities in awareness-raising initiatives can increase knowledge and participation in donation programmes.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of post-mortem time and temperature on microbiological safety, structural integrity, and biomaterial properties of human peroneus longus tendons.","authors":"Shangping Wang, Peng Chen, Dustin Mueller, R Glenn Hepfer, Haiyang Ma, Brooke Damon, Lia Campbell, Yongren Wu, Alyce Linthurst Jones, Kelvin G M Brockbank, Hai Yao","doi":"10.1007/s10561-026-10222-6","DOIUrl":"10.1007/s10561-026-10222-6","url":null,"abstract":"<p><p>Current tendon procurement practices follow the American Association of Tissue Banks (AATB) standards (15th Edition). With increasing demand for tendon allografts in the United States, extending the post-mortem interval (PMI) could enhance tissue availability for transplantation. This study evaluates the effects of extending PMIs up to 36 h on microbiological safety, tissue structure, and biomaterial properties of human peroneus longus (PL) tendon tissues, compared to current AATB standards. Our findings indicate that extending PMIs does not increase contamination risk, maintaining microbiological acceptability under extended warm ischemic conditions. Fixed charge density (FCD), an indicator of glycosaminoglycan (GAG) content, remained stable under extended PMIs and temperatures. Sirus red staining revealed a significant reduction in collagen intensity in warm tendons with extended PMIs, whereas cooling effectively preserved collagen content for up to 12 h beyond the standard. This collagen reduction may contribute to a disorganized matrix structure, facilitating increased water infiltration and greater porosity in the warm groups compared to the cooled groups. As microbiological, biomaterial and mechanical integrity remained uncompromised, our results suggest that extending PMI by up to 12 h, particularly under cooled conditions, is a viable approach to expanding the tendon donor pool for transplantation.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of reduced graphene oxide nanoparticles with linalyl acetate and lavender extract nanoemulsion on neural marker expression in human WJ-MSCs.","authors":"Mohsen Ghiasi, Fatemeh Kheirollahzadeh, Ali Zarei, Arash Padash, Hossein Dehghan, Marzieh Ghollasi","doi":"10.1007/s10561-026-10217-3","DOIUrl":"https://doi.org/10.1007/s10561-026-10217-3","url":null,"abstract":"<p><p>Wharton's jelly-derived human mesenchymal stem cells (WJ-MSCs) have received much attention in recent years due to their non-invasive isolation method and potential for allogeneic transplantation. This study assessed the differentiation of human WJ-MSCs into neuron-like cells using retinoic acid (RA), 3-isobutyl-1-methylxanthine (IBMX), in combination with reduced graphene oxide nanoparticles (rGO), linalyl acetate (LA), and lavender extract nanoemulsion (LEN). Lavender extract was obtained through sequential extraction using n-hexane, methanol, and ethyl acetate, followed by nanoemulsion preparation. The nanoemulsion properties were analyzed by dynamic light scattering (DLS), zeta potential measurement, and transmission electron microscopy (TEM). Optimal doses were determined via MTT assay for cell viability and Acridine Orange/Ethidium Bromide (AO/EB) staining for cell death detection. The expression of neuron-specific genes (NSE, MAP-2, β-tubulin III, and Oligo-2) was analyzed using quantitative Real-time PCR (qPCR). Furthermore, the protein levels of neuronal markers gamma-enolase (NSE), MAP-2, and β-tubulin III were assessed by immunocytochemistry (ICC) on days 7 and 14. Gene and protein expression analyses demonstrated that rGO-LEN and rGO-LA significantly (p-value ≤ 0.05) enhance the neural differentiation of human WJ-MSCs. These treatments induced a significant increase in the expression of neural markers (both at the gene and protein levels) on day 7 and especially on day 14 compared to the control group and other treatments. These findings can provide initial insights to guide and develop future research to achieve new and effective therapeutic approaches in the field of regenerative medicine and neuroregenerative research.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Optimizing a feasible protocol for acellular nerve allografts: An experimental study.","authors":"Marta de Juan Marín, Marta Pevida, Sara Llames, Juan Argüelles Luís, Daniel Camporro Fernández, Álvaro Meana","doi":"10.1007/s10561-026-10216-4","DOIUrl":"10.1007/s10561-026-10216-4","url":null,"abstract":"","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13035866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioglass nanofibers in a 3D scaffold orchestrate robust bone regeneration via enhanced osteogenesis and mineralization.","authors":"Nasrin Kheradmand, Morteza Alizadeh, Tayebe Artimani, Lobat Tayebi, Mehdi Azizi, Hadi Samadian","doi":"10.1007/s10561-026-10218-2","DOIUrl":"https://doi.org/10.1007/s10561-026-10218-2","url":null,"abstract":"<p><p>The main aim of the present study is to fabricate a 3D scaffold loaded with bioglass nanofibers to orchestrate robust bone regeneration via enhanced osteogenesis and mineralization. We applied a sol-gel method to fabricate the bioglass nanofibers and incorporated them into 3-D hydrogel (1%, 5%, and 10% wt.%). The fabricated scaffolds were characterized to determine their morphology, surface functional groups, porosity, swelling capacity, biodegradation profile, cytocompatibility, and hemocompatibility. Finally, the scaffolds were implanted in critical-size bone-defect models in the rabbit calvaria. The results showed that the calcination of precursor nanofibers to fabricate bioglass nanofibers reduced the nanofibers' diameter from around 981 nm to around 566 nm. Hydrogels containing 0%, 1%, and 5% BGNFs demonstrated no cytotoxicity, with viability percentages of 113%, 105.7%, and 112%, respectively. The 10% BGNF formulation exhibited a minor decrease in vitality (84.3%). Notably, the scaffolds promoted osteogenesis to a high degree, as evidenced by a concentration-dependent increase in mineral deposition under Alizarin Red staining. The animal studies showed that scaffold implantation supported new bone formation, as evidenced by histological analysis and micro-CT imaging. A denser, more interconnected bony network with superior mineralization (BMD) was the result of a remarkable rise in bone volume (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N), as well as a decrease in trabecular separation (Tb.Sp). The most significant outcomes were achieved when stem cells were combined with the BGNF scaffold. In summary, these results demonstrate that the bioactive nanocomposite scaffold is an excellent starting point for functional bone repair.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microenvironment-directed chondrogenesis of adipose-derived mesenchymal stem cells in hyaline, elastic, and fibrocartilage in vivo.","authors":"Yi Han, Hao Ma, Jingyi Chen, Liuhanghang Cheng, Ke Li, Baolin Zhang, Peiru Min","doi":"10.1007/s10561-026-10219-1","DOIUrl":"https://doi.org/10.1007/s10561-026-10219-1","url":null,"abstract":"<p><p>Cartilage exhibits remarkable subtype diversity-hyaline, fibrocartilage, and elastic-each with unique extracellular matrix architecture and mechanical function. Despite the clinical promise of adipose-derived mesenchymal stem cells (ADMSCs) for cartilage regeneration, whether distinct in vivo cartilage microenvironments can instruct ADMSC differentiation toward corresponding subtypes remains poorly defined. Green fluorescent protein-labeled human ADMSCs were encapsulated in a fibrin hydrogel and implanted into three representative cartilage sites-auricular (elastic), articular (hyaline), and meniscal (fibrocartilage)-in an immunodeficient rat model. Regenerated tissues were harvested at 4 weeks for histology (H&E), immunofluorescence analysis of hyaline (COL II, aggrecan), fibrocartilage (COL I, tenomodulin), and elastic (fibrillin-1, elastin) markers, and RT-PCR quantification of lineage-associated gene expression. ADMSCs survived and engrafted within all three microenvironments. Articular cartilage implants exhibited strong hyaline-like differentiation, characterized by intense COL II and aggrecan expression and upregulation of SOX9, COL2A1, and ACAN. Meniscal implants instead displayed a fibrocartilage-like profile with robust COL I and tenomodulin expression and elevated COL1A1, TNMD, and SCX transcripts. In contrast, auricular cartilage implants showed negligible expression of hyaline, fibrocartilage, or elastic markers, accompanied by global downregulation of chondrogenic and elastogenic genes. These results demonstrate that local cartilage niches exert distinct instructive effects on ADMSC fate: articular and meniscal environments effectively guide hyaline- and fibrocartilage-like differentiation, whereas the auricular niche under the tested conditions fails to support elastic or hyaline lineage commitment. Our findings highlight the decisive role of the tissue microenvironment in modulating ADMSC differentiation and underscore the need for bioengineered, niche-mimetic scaffolds to achieve subtype-specific cartilage regeneration-particularly for complex elastic tissues such as the auricle.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Willingness to bone allograft donation and transplantation in sub-Saharan Africa: a multi-country cross-sectional study.","authors":"Cédric Dongmo Mayopa, Aya Adelaïde Natacha Kouassi, Randy Buzisa Mbuku, André Claude Mbaga, Samuel Nkubamanu, Richard Ndambo, Gaspary Fodjeu, Jeholive Munoko, Loïc Fonkoue, Freddy Bombah, Pierre Louis Docquier, Thomas Schubert, Olivier Cornu","doi":"10.1007/s10561-026-10220-8","DOIUrl":"https://doi.org/10.1007/s10561-026-10220-8","url":null,"abstract":"<p><p>Bone is the second most frequently transplanted human tissue worldwide. In sub-Saharan Africa, donor and recipient consent for bone allograft donation and transplantation remains uncertain due to diverse sociocultural, religious, and socioeconomic contexts. This study assessed public attitudes toward bone allograft donation and transplantation in Cameroon, the Democratic Republic of Congo, and the Republic of Côte d'Ivoire to support the planning and implementation of bone banking programs. A multicountry cross-sectional survey was conducted between January and December 2024. Six hundred adults were recruited using a non-probabilistic, stratified convenience sampling in urban and rural settings. After informed consent, participants completed a structured questionnaire, self-administered whenever possible capturing sociodemographic data, willingness to donate and receive bone allografts, and perceived reasons for refusal. Descriptive analyses and univariate and multivariate logistic regression models were used to identify factors associated with willingness. The mean age of participants was 36.2 years. Overall, 72.5% reported willingness to donate bone allografts and 72.8% reported willingness to accept transplantation. The main reasons for refusal included belief-related or religious considerations, fear of trafficking, and expectations of financial compensation. In multivariable analyses, willingness to donate was independently associated with country of residence, religious affiliation, and occupation, while willingness to accept transplantation was associated with country and religious affiliation. Willingness to donate and receive bone allografts in the surveyed sub-Saharan Africa settings was relatively high although marked heterogeneity existed between countries. Key barriers include religious beliefs, mistrust of health systems, and socioeconomic vulnerability. The development of bone banking programs in the region will require culturally sensitive education, transparent governance, and active community engagement to transform expressed willingness into effective donation practices.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification and characterization of collagen in experimentally induced wounds treated with different types of platelet-rich plasma gel.","authors":"G Haro de Melo, F M Moreira, G G Mori, L F Bento, D V Barrionuevo, C B Laposy, R Giuffrida, A F F Rodrigues, G A T Ozaki, D A F da Silva, F L Pacagnelli, R M B Nogueira","doi":"10.1007/s10561-026-10215-5","DOIUrl":"10.1007/s10561-026-10215-5","url":null,"abstract":"<p><p>Due to its high concentration of growth factors, platelet-rich plasma (PRP) has become the subject of much research, either as an isolated or adjuvant treatment for wound repair. The fractal dimension has been used as an innovative method for the characterization and quantification of tissue structural alterations without any interference from the evaluator. The objective of this study was characterizing collagen types I and III in experimentally induced wounds and treated with different PRP sources by means of fractal dimension and other histological analyzes using blades stained with picrocirius-red and hematoxylin-eosin (HE). In statistical analysis, the variables presented a non-parametric distribution; thus, Mann-Whitney and Kruskall-Wallis tests (p < 0,05) were performed using the Student-Newman-Keuls method. It was observed an increase of the collagen fibers by measuring the area with Threshold Color in the different treatments, especially with autologous PRP (p = 0.009) and heterologous PRP (p = 0.016). The fractal dimension of type I collagen had a significant increase for the autologous group (p = 0.028) in relation to the control, but, on average (p < 0.05), type III collagen was higher than type I. Fractal dimension was efficient to separately characterize type I and type III collagen fibers. In this study, collagenization was higher in autologous when compared to heterologous and homologous PRP treatments.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12960424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing cadaver preparation protocol to reduce bacterial contamination in musculoskeletal allografts: a comparative study of battlefield fatalities and general population donors.","authors":"Helit Cohen, Moti Harats, Ron Burshtein, Alina Levi, Daniel Dothan, Marina BenShoshan, Michelle Cleary, Rachel Kornhaber, Josef Haik, Ayelet Di Segni","doi":"10.1007/s10561-026-10208-4","DOIUrl":"10.1007/s10561-026-10208-4","url":null,"abstract":"<p><p>Musculoskeletal allografts are becoming increasingly essential in orthopaedic and reconstructive surgery. A significant source of these allografts is tissues retrieved from deceased donors. While contributing substantially to the pool of allografts, these tissues are at increased risk of bacterial contamination. This risk is further amplified when tissues are procured from field casualties. In this study, we performed a side-by-side retrospective comparison of contamination rates in tissues retrieved from the general population and battlefield mortalities, representing field casualties. In response to the rising incidence of contaminated tissues obtained from field casualty donors, we implemented a more rigorous pre-operative washing protocol designed to maximize sterility. This protocol modification led to a significant decrease in the contamination ratio of tissues retrieved from field casualties. Our findings suggest that current pre-operative cadaver preparation guidelines may need to be reconsidered for tissue donations following unsanitary condition mortalities. Furthermore, these findings strongly suggest that field surgeries would benefit from a surgical pre-operative preparation designed specifically for incidents such as battlefields, accidents or mass casualty incident areas to minimize post-operative infections and improve surgical outcomes.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12935800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}