Cell and Tissue Banking最新文献_第10页

Manufacturing of CD34 + HPC-enriched, high-purity mononuclear cell products from umbilical cord blood. CD34的制造 + 来自脐带血的HPC富集的高纯度单核细胞产物。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-02-03 DOI: 10.1007/s10561-023-10070-8

Liam Wynn, Marnie Grace Wilson, Christopher Leonforte

{"title":"Manufacturing of CD34 + HPC-enriched, high-purity mononuclear cell products from umbilical cord blood.","authors":"Liam Wynn, Marnie Grace Wilson, Christopher Leonforte","doi":"10.1007/s10561-023-10070-8","DOIUrl":"10.1007/s10561-023-10070-8","url":null,"abstract":"The purpose of this study was to explore methods of selectively enriching CD34 + haematopoietic progenitor cells (HPC) in mononuclear cell (MNC) preparations, and to outline a procedure for cryopreservation and thawing of manufactured material. Density gradient centrifugation of umbilical cord blood was achieved using Ficoll-Paque™ media at 1.077 g/mL and 1.065 g/mL densities and Leucosep preparation tubes. Post-process samples were analysed for CD34 + and MNC content. Finally, MNCs were frozen down at a concentration of 8.5 × 106 cells/mL in CryoStor CS10 using an Asymptote VIAFreeze controlled rate freezer at a rate of - 2 °C per minute, then thawed and analysed for viability and recovery. Processing with 1.065 g/mL media selectively depleted non-HPC cell types, producing an approximately fourfold increase in CD34 + frequency (M ± 1SD = 1.4 ± 1.3%, P < 0.01) relative to the pre-process sample (M ± 1SD = 0.4 ± 0.3%), whereas 1.077 g/mL media produced only a twofold enrichment (0.7 ± 0.6, P < 0.01). This was not accompanied by any significant forfeit of CD34 + recovery (79 ± 32% vs. 78 ± 32% respectively; P = 0.87). The MNCs generated by the 1.065 g/mL procedure were of greater purity (96 ± 2%) than in the 1.077 g/mL procedure (80 ± 7%, P < 0.01). Post-thaw, MNC viability was 95 ± 1% and CD34 + viability was 98 ± 1%. Ultra-pure MNCs rich in CD34 + HPCs can be generated with a simple, inexpensive modification to Ficoll-Paque™ media. These products can be easily cryopreserved using a simple controlled rate freezing procedure.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"685-691"},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9209607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insufficiency of collagenases in establishment of primary chondrocyte culture from cartilage of elderly patients receiving total joint replacement. 胶原酶在从接受全关节置换术的老年患者软骨中建立原代软骨细胞培养中的不足。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-05-03 DOI: 10.1007/s10561-023-10094-0

Jiamin Mao, Lexi Huang, Yiyang Ding, Xiaoyu Ma, Quanming Wang, Lei Ding

{"title":"Insufficiency of collagenases in establishment of primary chondrocyte culture from cartilage of elderly patients receiving total joint replacement.","authors":"Jiamin Mao, Lexi Huang, Yiyang Ding, Xiaoyu Ma, Quanming Wang, Lei Ding","doi":"10.1007/s10561-023-10094-0","DOIUrl":"10.1007/s10561-023-10094-0","url":null,"abstract":"Background Collagenases are frequently used in chondrocyte isolation from articular cartilage. However, the sufficiency of this enzyme in establishing primary human chondrocyte culture remains unknown. Methods Cartilage slices shaved from femoral head or tibial plateau of patients receiving total joint replacement surgery (16 hips, 8 knees) were subjected to 0.02% collagenase IA digestion for 16 h with (N = 19) or without (N = 5) the pre-treatment of 0.4% pronase E for 1.5 h. Chondrocyte yield and viability were compared between two groups. Chondrocyte phenotype was determined by the expression ratio of collagen type II to I. The morphology of cultured chondrocytes was monitored with a light microscope.Results Cartilage with pronase E pre-treatment yielded significantly higher chondrocytes than that without the pre-treatment (3,399 ± 1,637 cells/mg wet cartilage vs. 1,895 ± 688 cells/mg wet cartilage; P = 0.0067). Cell viability in the former group was also significantly higher than that in the latter (94% ± 2% vs. 86% ± 6%; P = 0.03). When cultured in monolayers, cells from cartilage with pronase E pre-treatment grew in a single plane showing rounded shape while cells from the other group grew in multi-planes and exhibited irregular shape. The mRNA expression ratio of collagen type II to I was 13.2 ± 7.5 in cells isolated from cartilage pre-treated with pronase E, indicating a typical chondrocyte phenotype. Conclusions Collagenase IA was not sufficient in establishing primary human chondrocyte culture. Cartilage must be treated with pronase E prior to collagenase IA application.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"759-768"},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9411290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of MRI on stemness properties of Wharton's jelly-derived mesenchymal stem cells. MRI对Wharton氏胶状间充质干细胞干性的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10052-2

Mahnaz Tashakori, Fatemeh Asadi, Faezeh-Sadat Khorram, Azita Manshoori, Ali Hosseini-Chegeni, Fatemeh Mohseni Moghadam, Mahdieh Ahmadi Kamalabadi, Aliakbar Yousefi-Ahmadipour

{"title":"Effects of MRI on stemness properties of Wharton's jelly-derived mesenchymal stem cells.","authors":"Mahnaz Tashakori, Fatemeh Asadi, Faezeh-Sadat Khorram, Azita Manshoori, Ali Hosseini-Chegeni, Fatemeh Mohseni Moghadam, Mahdieh Ahmadi Kamalabadi, Aliakbar Yousefi-Ahmadipour","doi":"10.1007/s10561-022-10052-2","DOIUrl":"https://doi.org/10.1007/s10561-022-10052-2","url":null,"abstract":"Mesenchymal stem cells (MSCs), derived from various tissues, are served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton's jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. The aim of this study was to evaluate the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. Additionally, we assessed cell viability, growth kinetics and apoptosis of WJ-MSCs after MRI treatment. The quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) data showed that transcript levels of SOX-2, NANOG in MRI-treated WJ-MSCs were increased 32- and 213-fold, respectively. MTT assay was performed at 24, 48, and 72 h post-treatment and the viability was not significantly different between the two groups. The doubling time of the MRI group was markedly higher than the control group. In addition, the colony formation ability of WJ-MSCs after MRI treatment significantly increased. Furthermore, no change in apoptosis was seen before or after MRI treatment. Our results suggest that the use of MRI can improve the quality of MSCs and enhance the efficacy of mesenchymal stem cell-based therapies.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"24 3","pages":"523-533"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10106304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ophthalmic surface anesthetic alcaine on the proliferation and apoptosis of human corneal endothelial cells through HIF-1α regulation. 眼表面麻醉碱通过HIF-1α调控对人角膜内皮细胞增殖和凋亡的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10057-x

Quan Wang, Zhao Zhang, Xuesong Gao

{"title":"Effects of ophthalmic surface anesthetic alcaine on the proliferation and apoptosis of human corneal endothelial cells through HIF-1α regulation.","authors":"Quan Wang, Zhao Zhang, Xuesong Gao","doi":"10.1007/s10561-022-10057-x","DOIUrl":"https://doi.org/10.1007/s10561-022-10057-x","url":null,"abstract":"The corneal endothelium is a monolayer, which mediates solute and water flux across the posterior corneal surface. Alcaine's main component proparacaine is paramount in human corneal endothelium (HCE) cell regulation. This study explored the mechanism of alcaine in regulating HCE cells. HCE cell morphology under gradient concentrations was observed by an optical microscope. Cell proliferation and viability were detected by MTT assay to determine the half inhibitory concentration (IC 50). Cell apoptosis rate, HIF-1α mRNA expression, and HIF-1α, p/t-JNK and Caspase-3 protein levels were detected by flow cytometry, RT-qPCR, and Western blot. After treatment with alcaine at 0.625-5 g/L concentration range for 24 h, HCE cells showed cytoplasmic vacuolation, cell shrinkage, separation from culture matrix, and eventual death. Alcaine treated-HCE cell proliferation was decreased in a dose-dependent manner. The IC 50 of alcaine was 1.26 g/L. After alcaine treatment, HCE cell apoptosis rate was promoted and HIF-1α levels in HCE cells were stimulated. Knockdown of HIF-1α partially annulled the effects of alcaine on inhibiting HCE cell proliferation and facilitating apoptosis. Alcaine might activate the JNK/caspase-3 pathway by increasing HIF-1α. The inhibition of the JNK/caspase-3 pathway partially abrogated the effects of alcaine on inhibiting HCE cell proliferation and promoting apoptosis. Alcaine might affect HCE cell proliferation and apoptosis by upregulating HIF-1α and activating the JNK/caspase-3 pathway.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"24 3","pages":"561-570"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10115795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone marrow mesenchymal stem cells' osteogenic potential: superiority or non-superiority to other sources of mesenchymal stem cells? 骨髓间充质干细胞的成骨潜能:与其他来源的间充质干细胞相比优势还是劣势?

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10066-w

Mohammad Sadegh Gholami Farashah, Amirhossein Mohammadi, Maryam Javadi, Jafar Soleimani Rad, Seyed Kazem Shakouri, Shahla Meshgi, Leila Roshangar

{"title":"Bone marrow mesenchymal stem cells' osteogenic potential: superiority or non-superiority to other sources of mesenchymal stem cells?","authors":"Mohammad Sadegh Gholami Farashah, Amirhossein Mohammadi, Maryam Javadi, Jafar Soleimani Rad, Seyed Kazem Shakouri, Shahla Meshgi, Leila Roshangar","doi":"10.1007/s10561-022-10066-w","DOIUrl":"https://doi.org/10.1007/s10561-022-10066-w","url":null,"abstract":"Skeletal problems are an increasing issue due to the increase in the global aging population. Different statistics reports show that today, the global population is aging that results in skeletal problems, increased health system costs, and even higher mortality associated with skeletal problems. Common treatments such as surgery and bone grafts are not always effective and in some cases, they can even cause secondary problems such as infections or improper repair. Cell therapy is a method that can be utilized along with common treatments independently. Mesenchymal stem cells (MSCs) are a very important and efficient source in terms of different diseases, especially bone problems. These cells are present in different tissues such as bone marrow, adipose tissue, umbilical cord, placenta, dental pulp, peripheral blood, amniotic fluid and others. Among the types of MSCs, bone marrow mesenchymal stem cells (BMMSCs) are the most widely used source of these cells, which have appeared to be very effective and promising in terms of skeletal diseases, especially compared to the other sources of MSCs. This study focuses on the specific potential and content of BMMSCs from which the specific capacity of these cells originates, and compares their osteogenic potential with other types of MSCs, and also the future directions in the application of BMMSCs as a source for cell therapy.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"24 3","pages":"663-681"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10488884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Regenerative medicine improve neurodegenerative diseases. 再生医学改善神经退行性疾病。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10062-0

Seyedeh Sara Ashraf, Vahid Hosseinpour Sarmadi, Ghazaleh Larijani, Soheila Naderi Garahgheshlagh, Sara Ramezani, Soraya Moghadamifar, Seyedeh Lena Mohebi, Peiman Brouki Milan, Seyed Mohammad Amin Haramshahi, Nooshin Ahmadirad, Naser Amini

{"title":"Regenerative medicine improve neurodegenerative diseases.","authors":"Seyedeh Sara Ashraf, Vahid Hosseinpour Sarmadi, Ghazaleh Larijani, Soheila Naderi Garahgheshlagh, Sara Ramezani, Soraya Moghadamifar, Seyedeh Lena Mohebi, Peiman Brouki Milan, Seyed Mohammad Amin Haramshahi, Nooshin Ahmadirad, Naser Amini","doi":"10.1007/s10561-022-10062-0","DOIUrl":"https://doi.org/10.1007/s10561-022-10062-0","url":null,"abstract":"Regenerative medicine is a subdivision of medicine that improves methods to regrow, repair or replace unhealthy cells and tissues to return to normal function. Cell therapy, gene therapy, nanomedicine as choices used to cure neurodegenerative disease. Recently, studies related to the treatment of neurodegenerative disorders have been focused on stem cell therapy and Nano-drugs beyond other than regenerative medicine. Hence, by data from experimental models and clinical trials, we review the impact of stem cell therapy, gene therapy, and nanomedicine on the treatment of Alzheimer's disease (AD), Parkinson's disease (PD), and Amyotrophic lateral sclerosis (ALS). Indeed, improved knowledge and continued research on gene therapy and nanomedicine in treating Alzheimer's disease, Parkinson's disease, and Amyotrophic lateral sclerosis lead to advancements in effective and practical treatments for neurodegenerative diseases.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"24 3","pages":"639-650"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10106309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Vascular allografts for clinical application in Europe: assessment of 30 years of experience with vascular tissue banking in Brussels. 同种异体血管移植在欧洲的临床应用:评估30年的经验与血管组织银行在布鲁塞尔。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10063-z

Ramadan Jashari, Vanessa Bouzet, Maria-Josee Alcaraz Blanco, Alison Oleffe, Emilie Lecocq, Stefano Mastrobuoni

{"title":"Vascular allografts for clinical application in Europe: assessment of 30 years of experience with vascular tissue banking in Brussels.","authors":"Ramadan Jashari, Vanessa Bouzet, Maria-Josee Alcaraz Blanco, Alison Oleffe, Emilie Lecocq, Stefano Mastrobuoni","doi":"10.1007/s10561-022-10063-z","DOIUrl":"https://doi.org/10.1007/s10561-022-10063-z","url":null,"abstract":"Vascular tissue banking has been carried out in Brussels for over 30 years in compliance with EU and Swiss tissue banking regulations. A total of 2.765 vascular tissue donations were performed in Belgian, French, Netherlands and Suisse transplant centres: 547(20%), 1.013(37%) and 1.205(43%) during the first, second and third periods, respectively. 85% and 18% increase in donations during the second and third decades compared to previous one, were remarkable. Of the 7.066 evaluated vascular tissues, 2.407(227, 921 and 1.259) were discarded (34.1%), whereas 4.659(523, 1.861 and 2.275) accepted (65.9%) during the respective period. Of the 92 donated veins, 44(47.8%) were discarded and 48(52.2%) accepted. Allografts available for clinical application were stored in vapours of liquid nitrogen. A total of 4.636 allografts were delivered and transplanted for cases of infection (58%), critical limb ischaemia (16%) and congenital cardiac surgery (15%). Thirty veins were implanted. The progressive increases in donations of 20%, 37% and 43% and in transplantations of 20.8%, 34.6% and 45% during the first, second and third periods, respectively, were remarkable. Complications were reported after transplantation and these included acute rejection of two femoral arteries one month after transplantation. We conclude that the donation and transplantation of cryopreserved vascular allografts was stable with a progressive increase over time. Allografts were used predominantly for the treatment of infection, limb salvage for critical ischaemia and for neonates and infants with congenital cardiac malformation. Immune related rejection was observed. This should be a subject of future investigation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"24 3","pages":"613-625"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9809507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10106313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cord blood derived NK cells activated in counter with tumor cells. 脐带血NK细胞活化与肿瘤细胞对抗。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10056-y

Behnaz Valipour, Ghazal Majidi, Khadijeh Dizaji Asl, Hojjatollah Nozad Charoudeh

引用次数: 0

Drivers of consumers' behavioral intention toward private umbilical cord blood banking: a review. 消费者对私人脐带血银行行为意向的驱动因素:综述。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10064-y

Neda Saraygord-Afshari, Sonia Ghayem, Reza Foudazi, Majid Safa

{"title":"Drivers of consumers' behavioral intention toward private umbilical cord blood banking: a review.","authors":"Neda Saraygord-Afshari, Sonia Ghayem, Reza Foudazi, Majid Safa","doi":"10.1007/s10561-022-10064-y","DOIUrl":"https://doi.org/10.1007/s10561-022-10064-y","url":null,"abstract":"Immunitary bioeconomy encompasses a significant share of the bioeconomy that is accompanied by a ‎high degree of complexity and various religious and ethical controversies for both customers and the ‎service providers. Compared to blood banking, these complexities are more substantial for the ‎new state-of-the-art technology of umbilical cord blood (UCB) banking, in which the viable ‎therapeutically active substance of cord blood (i.e., cord blood stem cells (CBSCs)) is banked for much ‎less likely future demand. It became even more complicated when we knew that the main three types ‎of cord blood banking industry (i.e., private, public, or hybrid models) are not the same regarding ‎economic, ‎ethical, and even social considerations. The present paper aims to review and discuss the ‎main drivers of behavioral intention among the customers of private UCB banking. We focused on ‎private UCB banking because, although there is a low likelihood of childs' future need for their siblings' ‎CBSCs, there is an unnecessary growing demand for using private UCB banking services. Based on the ‎previously published pieces of research, we discussed five main influential factors (i.e., awareness, ‎reference group, usability, disease history, and price) that can affect the customers' risk perception ‎‎(and further their behavioral intention) to preserve their child UCB for private applications. Finally, we ‎concluded that private UCB banking must not be considered a commercial activity, and ethically healthcare ‎managers must be more actively involved in ‎facilitating the proper flow of information among the ‎customers.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"24 3","pages":"651-661"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10115040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low rate of detection of SARS-CoV-2 RNA in deceased tissue donors. 死亡组织供体中SARS-CoV-2 RNA检出率低。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10054-0

Melissa A Greenwald, Eduard Grebe, Valerie Green, Alyce Linthurst Jones, Jeffrey M Linnen, Phillip Williamson, Michael P Busch, Matthew J Kuehnert

{"title":"Low rate of detection of SARS-CoV-2 RNA in deceased tissue donors.","authors":"Melissa A Greenwald, Eduard Grebe, Valerie Green, Alyce Linthurst Jones, Jeffrey M Linnen, Phillip Williamson, Michael P Busch, Matthew J Kuehnert","doi":"10.1007/s10561-022-10054-0","DOIUrl":"https://doi.org/10.1007/s10561-022-10054-0","url":null,"abstract":"Given the possibility for disease transmission, this study was performed to determine whether there is detectable SARS-CoV-2 viral RNA in the blood of deceased tissue donors. A retrospective analysis of blood samples from eligible deceased tissue donors from Oct 2019 through June 2020 was performed. Plasma aliquots were initially tested with a SARS-CoV-2 NAT Assay; positive samples were further tested using an alternate NAT and an antibody assay. The proportion of donors with confirmed RNAemia and 95% confidence intervals were computed. Of donor samples collected in 2019, 894 yielded valid results, with 6 initially positive, none of which confirmed positive by alternate NAT. Of donor samples collected in 2020, 2562 yielded valid initial NAT results, with 21 (0.8%) initially positive. Among those, 3 were confirmed by alternate NAT, 17 were not confirmed, and 1 had an invalid alternate NAT result. The rate of SARS-CoV-2 RNAemia in deceased tissue donors is approximately 1 per 1000, and it is unknown whether this RNAemia reflects the presence of infectious virus. Given these results, the risk of transmission through tissue is thought likely to be low.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"24 3","pages":"585-596"},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10170249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1