Cell and Tissue Banking最新文献_第10页

How to counteract the lack of donor tissue in cardiac surgery? Initial experiences with a newly established homograft procurement program. 如何解决心脏手术中供体组织缺乏的问题？新建立的同种移植组织采购项目的初步经验。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2023-04-25 DOI: 10.1007/s10561-023-10087-z

Martin O Schmiady, Ramadan Jashari, Renato Lenherr, Stefan Regenscheit, Dave Hitendu, Martin Wendt, Stefanie Schiess, Martin Schweiger, Michael Hofmann, Juri Sromicki, Andreas Flammer, Markus J Wilhelm, Robert Cesnjevar, Thierry Carrel, Paul R Vogt, Carlos A Mestres

{"title":"How to counteract the lack of donor tissue in cardiac surgery? Initial experiences with a newly established homograft procurement program.","authors":"Martin O Schmiady, Ramadan Jashari, Renato Lenherr, Stefan Regenscheit, Dave Hitendu, Martin Wendt, Stefanie Schiess, Martin Schweiger, Michael Hofmann, Juri Sromicki, Andreas Flammer, Markus J Wilhelm, Robert Cesnjevar, Thierry Carrel, Paul R Vogt, Carlos A Mestres","doi":"10.1007/s10561-023-10087-z","DOIUrl":"10.1007/s10561-023-10087-z","url":null,"abstract":"Homograft heart valves may have significant advantages and are preferred for the repair of congenital valve malformations, especially in young women of childbearing age, athletes and in patients with active endocarditis. A growing problem, however, is the mismatch between tissue donation and the increasing demand. The aim of this paper is to describe the initiation process of a homograft procurement program to attenuate the shortage of organs. A comprehensive description of the infrastructure and procedural steps required to initiate a cardiac and vascular tissue donation program combined with a prospective follow-up of all homografts explanted at our institution. Between January 2020 and May 2022, 28 hearts and 12 pulmonary bifurcations were harvested at our institution and delivered to the European homograft bank. Twenty-seven valves (19 pulmonary valves, 8 aortic valves) were processed and allocated for implantation. The reasons for discarding a graft were either contamination (n = 14), or morphology (n = 13) or leaflet damage (n = 2). Five homografts (3 PV, 2 AV) have been cryopreserved and stored while awaiting allocation. One pulmonary homograft with a leaflet cut was retrieved by bicuspidization technique and awaits allocation, as a highly requested small diameter graft. The implementation of a tissue donation program in cooperation with a homograft bank can be achieved with reasonable additional efforts at a transplant center with an in-house cardiac surgery department. Challenging situations with a potential risk of tissue injury during procurement include re-operation, harvesting by a non-specialist surgeon and prior central cannulation for mechanical circulatory support.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"1-10"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9356765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of storage time prior to cryopreservation on mechanical properties of aortic homografts. 低温保存前的储存时间对主动脉同种移植物机械性能的影响

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2023-02-27 DOI: 10.1007/s10561-023-10079-z

Ida Axelsson, Anna Gustafsson, Hanna Isaksson, Johan Nilsson, Torsten Malm

{"title":"Impact of storage time prior to cryopreservation on mechanical properties of aortic homografts.","authors":"Ida Axelsson, Anna Gustafsson, Hanna Isaksson, Johan Nilsson, Torsten Malm","doi":"10.1007/s10561-023-10079-z","DOIUrl":"10.1007/s10561-023-10079-z","url":null,"abstract":"Optimal time spans in homograft procurement are still debatable among tissue banks and needs to be further investigated. Cell viability decreases at longer preparation intervals, but the effect on collagen and elastic fibers has not been investigated to the same extent. These fibers are of importance to the homograft elasticity and strength. The objective of this study was to analyze the mechanical properties of homograft tissue at different time spans in the procurement process. Ten aortic homografts were collected at the Tissue Bank in Lund. Twelve samples were obtained from each homograft, cryopreserved in groups of three after 2-4 days, 7-9 days, 28-30 days, and 60-62 days in antibiotic decontamination. Mechanical testing was performed with uniaxial tensile tests, calculating elastic modulus, yield stress and energy at yield stress. Two randomly selected samples were assessed with light microscopy. Procurement generated a total of 120 samples, with 30 samples in each time group. Elastic modulus and yield stress was significantly higher in samples cryopreserved after 2-4 days (2.7 MPa (2.5-5.0) and 0.78 MPa (0.68-1.0)) compared to 7-9 days (2.2 MPa (2.0-2.6) and 0.53 MPa (0.46-0.69)), p = 0.008 and 0.011 respectively. Light microscopy did not show any difference in collagen and elastin at different time spans. There was a significant decrease in elastic modulus and yield stress after 7 days of decontamination at 4 °C compared to 2-4 days. This could indicate some deterioration of elastin and collagen at longer decontamination intervals. Clinical significance of these findings remains to be clarified.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"27-37"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10902001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9663361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Impact of storage time prior to cryopreservation on mechanical properties of aortic homografts. 更正：低温保存前的储存时间对主动脉同种移植物机械性能的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 DOI: 10.1007/s10561-023-10085-1

Ida Axelsson, Anna Gustafsson, Hanna Isaksson, Johan Nilsson, Torsten Malm

引用次数: 0

Hyaluronic acid/silicon nanoparticle scaffold induces proliferation and differentiation of mouse spermatogonial stem cells transplanted to epididymal adipose tissue. 透明质酸/硅纳米颗粒支架可诱导移植到附睾脂肪组织的小鼠精原干细胞增殖和分化。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2023-09-07 DOI: 10.1007/s10561-023-10093-1

Saber Saharkhiz, Zohreh Abdolmaleki, Mohammad Amin Eslampour

{"title":"Hyaluronic acid/silicon nanoparticle scaffold induces proliferation and differentiation of mouse spermatogonial stem cells transplanted to epididymal adipose tissue.","authors":"Saber Saharkhiz, Zohreh Abdolmaleki, Mohammad Amin Eslampour","doi":"10.1007/s10561-023-10093-1","DOIUrl":"10.1007/s10561-023-10093-1","url":null,"abstract":"Spermatogonia stem cells (SSCs) are a unique cell population maintaining male spermatogenesis during life, through their potential for proliferation and differentiation. The application of silicon nanoparticles (SNs) and hyaluronic acid (HA) to induce the differentiation of SSCs seems promising. Herein, we investigate the effect of SN and HA scaffolds on the progression of SSCs spermatogenesis in mice. Initially SSCs were isolated from healthy immature mice and cultured on prepared scaffolds (HA, SN, and HA/SN) in a 3D culture system. Then viability of SSCs cultured on scaffolds was examined using MTT assay and Acridine Orange staining. Then SSCs cultured on scaffolds were transplanted into epididymal adipose tissue (EAT) in mature mice and the result was studied by H&E and IHC staining 8 weeks after transplantation. MTT and Acridine Orange analysis revealed that among three different scaffolds HA/SN based scaffold causes considerable toxicity on SSCs (P < 0.05) while H&E staining showed that culture of SSCs on HA, SN, and HA/SN scaffolds has a positive effect on the progression of SSCs spermatogenesis after transplantation into EAT. IHC staining identified TP1, TEKT1, and PLZF as crucial biomarkers in the spermatogenesis development of SSCs transplanted to EAT. According to the presence of these biomarkers in different experimental groups, we found the most spermatogenesis development in SSCs cultured on HA/SN scaffold (PLZF, P < 0.01) (TEKT1, P < 0.01) (TP1, P < 0.001). Our study showed that, although the cytotoxic effect of the HA/SN scaffold decreases the viability rate of SSCs; however, SSCs that survive on HA/SN scaffold showed more ability to progress in spermatogenesis after transplantation into EAT.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"231-243"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10171856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreserved amniotic membrane in chronic nonhealing wounds: a series of case reports. 冷冻保存羊膜在慢性不愈合伤口中的应用：一系列病例报告。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2023-11-09 DOI: 10.1007/s10561-023-10100-5

Vojtech Horvath, Alzbeta Svobodova, Joao Victor Cabral, Petr Stadler, Jaroslav Lindner, Miluse Berka Mrstinova, Lukas Balogh, Katerina Jirsova

{"title":"Cryopreserved amniotic membrane in chronic nonhealing wounds: a series of case reports.","authors":"Vojtech Horvath, Alzbeta Svobodova, Joao Victor Cabral, Petr Stadler, Jaroslav Lindner, Miluse Berka Mrstinova, Lukas Balogh, Katerina Jirsova","doi":"10.1007/s10561-023-10100-5","DOIUrl":"10.1007/s10561-023-10100-5","url":null,"abstract":"A case series of the use of amniotic membrane (AM) for treating chronic nonhealing wounds. It presents five cases of polymorbid patients with a total of nine chronic nonhealing wounds. The patient group consisted of four men and one woman with various comorbidities, aged 45-72 years. The mean initial wound size was 15.8 cm2, and the mean time from the onset of the wound to the first application of AM was 122 weeks. The wounds were caused by chronic venous insufficiency and/or peripheral arterial disease. Wounds were treated in a standardized protocol. AM was applied weekly in the first month and then every two weeks. Photo documentation of the wound and microbiological colonization was carried out at each visit. In three out of five patients, the AM treatment effectively promoted healing up to complete wound closure. In two cases, the wounds stayed unhealed despite numerous AM applications. Pain relief was noted in all patients. The success of the treatment was closely tied to patient factors, such as adherence to the prescribed treatment regimen and individual patient characteristics. In some cases, treatment failure was observed, possibly due to underlying comorbidities, wound parameters, or poor patient compliance. AM treatment has the potential to become a viable treatment option for these nonhealing wounds. However, the effectiveness of the treatment may be influenced by various patient factors and the underlying cause of the wound. Therefore, it is crucial to have an individualized treatment plan that considers these particular factors.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"325-337"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10901998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72013635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and characterization of a bovine collagen: GAG scaffold with Uruguayan raw material for tissue engineering. 牛胶原蛋白的合成与表征：用乌拉圭原料制成的组织工程用 GAG 支架。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2021-09-18 DOI: 10.1007/s10561-021-09960-6

L Pereira, L Echarte, M Romero, G Grazioli, H Pérez-Campos, A Francia, W Vicentino, A W Mombrú, R Faccio, I Álvarez, C Touriño, H Pardo

{"title":"Synthesis and characterization of a bovine collagen: GAG scaffold with Uruguayan raw material for tissue engineering.","authors":"L Pereira, L Echarte, M Romero, G Grazioli, H Pérez-Campos, A Francia, W Vicentino, A W Mombrú, R Faccio, I Álvarez, C Touriño, H Pardo","doi":"10.1007/s10561-021-09960-6","DOIUrl":"10.1007/s10561-021-09960-6","url":null,"abstract":"Tissue engineering (TE) and regenerative medicine offer strategies to improve damaged tissues by using scaffolds and cells. The use of collagen-based biomaterials in the field of TE has been intensively growing over the past decades. Mesenchymal stromal cells (MSCs) and dental pulp stem cells (DPSCs) are promising cell candidates for development of clinical composites. In this study, we proposed the development of a bovine collagen type I: chondroitin-6-sulphate (CG) scaffold, obtained from Uruguayan raw material (certified as free bovine spongiform encephalopathy), with CG crosslinking enhancement using different gamma radiation doses. Structural, biomechanical and chemical characteristics of the scaffolds were assessed by Scanning Electron Microscopy, axial tensile tests, FT-IR and Raman Spectroscopy, respectively. Once we selected the most appropriate scaffold for future use as a TE product, we studied the behavior of MSCs and DPSCs cultured on the scaffold by cytotoxicity, proliferation and differentiation assays. Among the diverse porous scaffolds obtained, the one with the most adequate properties was the one exposed to 15 kGy of gamma radiation. This radiation dose contributed to the crosslinking of molecules, to the formation of new bonds and/or to the reorganization of the collagen fibers. The selected scaffold was non-cytotoxic for the tested cells and a suitable substrate for cell proliferation. Furthermore, the scaffold allowed MSCs differentiation to osteogenic, chondrogenic, and adipogenic lineages. Thus, this work shows a promising approach to the synthesis of a collagen-scaffold suitable for TE.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"123-142"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39427537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of oxygen tension in cell fate and regenerative medicine: implications of hypoxia/hyperoxia and free radicals. 氧张力在细胞命运和再生医学中的作用：缺氧/过氧和自由基的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2023-06-26 DOI: 10.1007/s10561-023-10099-9

Mehdi Rasouli, Roya Fattahi, Ghader Nuoroozi, Zeinab Zarei-Behjani, Maliheh Yaghoobi, Zeinab Hajmohammadi, Simzar Hosseinzadeh

{"title":"The role of oxygen tension in cell fate and regenerative medicine: implications of hypoxia/hyperoxia and free radicals.","authors":"Mehdi Rasouli, Roya Fattahi, Ghader Nuoroozi, Zeinab Zarei-Behjani, Maliheh Yaghoobi, Zeinab Hajmohammadi, Simzar Hosseinzadeh","doi":"10.1007/s10561-023-10099-9","DOIUrl":"10.1007/s10561-023-10099-9","url":null,"abstract":"Oxygen pressure plays an integral role in regulating various aspects of cellular biology. Cell metabolism, proliferation, morphology, senescence, metastasis, and angiogenesis are some instances that are affected by different tensions of oxygen. Hyperoxia or high oxygen concentration, enforces the production of reactive oxygen species (ROS) that disturbs physiological homeostasis, and consequently, in the absence of antioxidants, cells and tissues are directed to an undesired fate. On the other side, hypoxia or low oxygen concentration, impacts cell metabolism and fate strongly through inducing changes in the expression level of specific genes. Thus, understanding the precise mechanism and the extent of the implication of oxygen tension and ROS in biological events is crucial to maintaining the desired cell and tissue function for application in regenerative medicine strategies. Herein, a comprehensive literature review has been performed to find out the impacts of oxygen tensions on the various behaviors of cells or tissues.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"195-215"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10045418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and biocompatibility evaluation of acellular rat skin scaffolds for skin tissue engineering applications. 用于皮肤组织工程应用的细胞大鼠皮肤支架的特性和生物相容性评估。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2023-09-03 DOI: 10.1007/s10561-023-10109-w

Leila Taghizadeh Momen, Arash Abdolmaleki, Asadollah Asadi, Saber Zahri

{"title":"Characterization and biocompatibility evaluation of acellular rat skin scaffolds for skin tissue engineering applications.","authors":"Leila Taghizadeh Momen, Arash Abdolmaleki, Asadollah Asadi, Saber Zahri","doi":"10.1007/s10561-023-10109-w","DOIUrl":"10.1007/s10561-023-10109-w","url":null,"abstract":"Utilization of acellular scaffolds, extracellular matrix (ECM) without cell content, is growing in tissue engineering, due to their high biocompatibility, bioactivity ad mechanical support. Hence, the purpose of this research was to study the characteristics and biocompatibility of decellularized rat skin scaffolds using the osmotic shock method. First, the skin of male Wistar rats was harvested and cut into 1 × 1 cm2 pieces. Then, some of the harvested parts were subjected to the decellularization process by applying osmotic shock. Comparison of control and scaffold samples was conducted in order to assure cell elimination and ECM conservation by means of histological evaluations, quantification of biochemical factors, measurement of DNA amount, and photographing the ultrastructure of the samples by scanning electron microscopy (SEM). In order to evaluate stem cell viability and adhesion to the scaffold, adipose-derived mesenchymal stem cells (AD-MSCs) were seeded on the acellular scaffolds. Subsequently, MTT test and SEM imaging of the scaffolds containing cultured cells were applied. The findings indicated that in the decellularized scaffolds prepared by osmotic shock method, not only the cell content was removed, but also the ECM components and its ultrastructure were preserved. Also, the 99% viability and adhesion of AD-MSCs cultured on the scaffolds indicate the biocompatibility of the decellularized skin scaffold. In conclusion, decellularized rat skin scaffolds are biocompatible and appropriate scaffolds for future investigations of tissue engineering applications.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"217-230"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10148386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of healthcare professionals to improve organ donation and transplantation outcome: a national study. 医护人员在改善器官捐赠和移植结果方面的作用：一项全国性研究。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2023-01-28 DOI: 10.1007/s10561-023-10071-7

Shadnoush Mahdi, Latifi Marzieh, Rahban Habib, Pourhosein Elahe, Dehghani Sanaz

{"title":"The role of healthcare professionals to improve organ donation and transplantation outcome: a national study.","authors":"Shadnoush Mahdi, Latifi Marzieh, Rahban Habib, Pourhosein Elahe, Dehghani Sanaz","doi":"10.1007/s10561-023-10071-7","DOIUrl":"10.1007/s10561-023-10071-7","url":null,"abstract":"Background: Improve the quality of donor coordination activities caused by an increased organ donation rate. The aim of this study was to assess the influence of organ donation coordinators' characteristics on rate of donation and family consent rate in Recognition Centers (RCs) and Organ Procurement Units (OPUs) in Iran by analyzing the organ procurement and transplantation data.Materials and methods: Based on a questionnaire, this retrospective study evaluated the number of confirmed brain deaths, family consent rate, organ recoveries, rate of expired brain death cases before and after family consent in Iran.Results: According to results, the overall family consent rate in the entire country is equal to 60.63%. The work experience had a significant effect on the number of procured organs (P < 0.004), death rate after family consent (P < 0.04), and eligible donor death before family consent (P < 0.03). The type of unit (RCs or OPUs) had significant difference on death after family consent (P < 0.023), the death before family consent (P < 0.014), the sum of procured organ (P < 0.04).Conclusion: The consent rate and donor management in the cases of brain death are unacceptable. The coordinators need training to increase their efficiency in terms of family approach and maintenance of brain death. Only by improving the level of family consent and increasing the coordinators' maintenance skills for brain death cases can the amount of organ donation in Iran be doubled to the current amount.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"159-165"},"PeriodicalIF":1.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10584641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of CM-Dil-labeled Muse cells in culture and in skin wounds in rats. CM-Dil 标记的 Muse 细胞在大鼠培养和皮肤伤口中的特性。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-03-01 Epub Date: 2023-01-08 DOI: 10.1007/s10561-022-10067-9

Yan-Yun Cao, Jing Ning, Ru-Zhi Zhang, Kang Ge, Ting-Ting Huang

{"title":"Characterization of CM-Dil-labeled Muse cells in culture and in skin wounds in rats.","authors":"Yan-Yun Cao, Jing Ning, Ru-Zhi Zhang, Kang Ge, Ting-Ting Huang","doi":"10.1007/s10561-022-10067-9","DOIUrl":"10.1007/s10561-022-10067-9","url":null,"abstract":"To investigate the characteristics of multilineage-differentiating stress-enduring (Muse) cells labeled with chloromethyl dialkylcarbocyanine (CM-Dil) in culture and in skin wounds of rats. Normal human dermal fibroblasts (NHDFs) were obtained from foreskins and were confirmed by immunocytochemistry with vimentin. Muse cells were derived from NHDFs using long-term trypsinization (LTT), were confirmed using immunocytochemistry with antibodies against stage specific embryonic antigen-3 (SSEA-3) and CD105 and were expanded in suspension cultures. The Muse cells were labeled with CM-Dil and were further evaluated with respect to their biological properties using CCK-8 assays and scratch tests. One hundred µl CM-Dil-labeled Muse cells at a concentration of 5 × 103/µl were injected subcutaneously at the edges of skin wounds in adult male SD rats. At weeks 1, 3 and 5 after the injection, the distribution of CM-Dil-labeled Muse cells in skin tissues was observed using immunofluorescence microscopy. Muse cells were double-positive for CD105 and SSEA-3. ALP staining of the M-clusters were positive and they displayed orange-red fluorescence after labelling with CM-Dil, which had no adverse effects on their viability, migration or differentiation capacity. One week after the subcutaneous injection of CM-Dil-labeled Muse cells, many cells with orange-red fluorescence were observed at the edges of the skin injuries; those fluorescent spots gradually decreased over time, and only a few Muse cells with fluorescence could be detected by week 5. CM-Dil can be used to label Muse cells without affecting their proliferation, migration or differentiation, and can be used for short-term tracking of Muse cells for the treatment of skin wounds in a rat model.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"285-294"},"PeriodicalIF":1.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10844249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0