Cell and Tissue Banking最新文献_第9页

Characterization of three washing/decellularization procedures for the production of bioactive human micronized neural tissue (hMINT). 用于生产具有生物活性的人类微粉化神经组织（hMINT）的三种洗涤/脱细胞程序的表征。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-02-28 DOI: 10.1007/s10561-023-10075-3

Gaëtan J-R Delcroix, Amber Hackett, Paul C Schiller, H Thomas Temple

{"title":"Characterization of three washing/decellularization procedures for the production of bioactive human micronized neural tissue (hMINT).","authors":"Gaëtan J-R Delcroix, Amber Hackett, Paul C Schiller, H Thomas Temple","doi":"10.1007/s10561-023-10075-3","DOIUrl":"10.1007/s10561-023-10075-3","url":null,"abstract":"Background: We developed a novel, injectable and decellularized human peripheral nerve-based scaffold, named Micronized Human Neural Tissue (hMINT), designed to be used as a supportive matrix for stem cell transplantation in the context of spinal cord injury (SCI).Materials and methods: Human donated sciatic nerves were micronized at liquid nitrogen temperature prior to decellularization using 3 different procedures of various harshness. hMINT were characterized in terms of particle size, DNA, sulfated glycosaminoglycans (sGAG) and growth factors content. To test the biocompatibility and bioactivity of the various preparations, we used a type of mesenchymal stromal cells (MSCs), termed MIAMI cells, which were placed in contact with hMINT to monitor cell attachment by confocal microscopy and gene expression by RT-qPCR in vitro.Results: The content of DNA, sGAG and growth factors left in the product after processing was highly dependent on the decellularization procedure used. We demonstrated that hMINT are biocompatible and promoted the attachment and long-term survival of MIAMI cells in vitro. Finally, combination with hMINT increased MIAMI cells mRNA expression of pro-survival and anti-inflammatory factors. Importantly, the strongest bioactivity on MIAMI cells was observed with the hMINT decellularized using the mildest decellularization procedure, therefore emphasizing the importance of achieving an adequate decellularization without losing the hMINT's bioactivity.Perspectives and clinical significance: The capacity of hMINT/stem cells to facilitate protection of injured neural tissue, promote axon re-growth and improve functional recovery will be tested in an animal model of SCI and other neurodegenerative disorders in the future.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10803027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of hypoxia on the expression of microRNA in extracellular vesicles of human umbilical cord stem cells in vitro. 缺氧对体外培养的人脐带干细胞胞外小泡中微小RNA表达的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-05-24 DOI: 10.1007/s10561-023-10095-z

Huifen Zang, Zhaohui Wang, Qingqing Wu, Lei Shi, Ge Chen

{"title":"Effect of hypoxia on the expression of microRNA in extracellular vesicles of human umbilical cord stem cells in vitro.","authors":"Huifen Zang, Zhaohui Wang, Qingqing Wu, Lei Shi, Ge Chen","doi":"10.1007/s10561-023-10095-z","DOIUrl":"10.1007/s10561-023-10095-z","url":null,"abstract":"Mesenchymal stem cells (MSCs) derived extracellular vesicles, which have been shown to possess therapeutic effects for many diseases. However, how hypoxic conditions would affect exosomal microRNA expression in human umbilical cord MSCs (hUC-MSCs) is currently not investigated. This study aims to investigate the potential function of in vitro microRNAs of hUC-MSC cultured under normoxic and hypoxic conditions. Extracellular vesicles secreted from hUC-MSCs cultured in normoxic (21% O2) and hypoxic (5% O2) conditions were collected for microRNA identification. Zeta View Laser Scattering and transmission electron microscopy were used to observe the size and morphology of extracellular vesicles. qRT-PCR was performed to measure the expression of related microRNAs. The Gene Ontology and KEGG pathway were used to predict the function of microRNAs. Finally, the effects of hypoxia on the expression of related mRNAs and cellular activity were examined. This study identified 35 upregulated and 8 downregulated microRNAs in the hypoxia group. We performed target genes analysis to explore the potential function of these microRNA upregulated in the hypoxia group. Significant enrichment of the cell proliferation, pluripotency of stem cells, MAPK, Wnt, and adherens junction pathways were observed in the GO and KEGG pathways. Under hypoxic conditions, the expression levels of 7 target genes were lower than that of the normal environment. In conclusion, this study demonstrated for the first time that microRNA expression in extracellular vesicles of human umbilical vein stem cells cultured under hypoxia is different from that under normal conditions, and these microRNAs may be markers for detecting hypoxia.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9510085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of demineralized bone matrix with different cycling crushing times in posterolateral fusion model of athymic rats. 无胸腺大鼠后外侧融合模型中不同循环粉碎时间脱矿骨基质的比较。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-05-03 DOI: 10.1007/s10561-023-10086-0

Shao-Lin Ji, Xiao-Dan Zhao, Li-Min Wang, Cheng-Gang Pang, Wen-Jing Li, Kun-Xiu Song, Rong-Xing Ma, Rui-Feng Li, Jing-Yu Zhang, Yong-Cheng Hu

{"title":"Comparison of demineralized bone matrix with different cycling crushing times in posterolateral fusion model of athymic rats.","authors":"Shao-Lin Ji, Xiao-Dan Zhao, Li-Min Wang, Cheng-Gang Pang, Wen-Jing Li, Kun-Xiu Song, Rong-Xing Ma, Rui-Feng Li, Jing-Yu Zhang, Yong-Cheng Hu","doi":"10.1007/s10561-023-10086-0","DOIUrl":"10.1007/s10561-023-10086-0","url":null,"abstract":"Decalcified bone matrix (DBM) is a widely used alternative material for bone transplantation. In the DBM production process, an effective particle size and the highest utilization rate of raw materials can be achieved only through multiple high-speed circulating comminution. The rat posterolateral lumbar fusion model (PLF) is the most mature small animal model for the initial evaluation of the efficacy of graft materials for bone regeneration and spinal fusion. To evaluate the differences in the in vivo osteogenic effects of DBM pulverization through 1, 5, 9, and 14 high-speed cycles, sixty athymic rats were divided into six groups: single cycling crushing (CC1), 5 cycles of crushing (CC5), 9 cycles of crushing (CC9), 13 cycles of crushing (CC13), autogenous bone graft (ABG) and negative control (NC). Posterolateral lumbar fusion was performed. Six weeks after surgery, the bilateral lumbar fusion of athymic rats was evaluated through manual palpation, X-ray, micro-CT and histological sections. Rank data were tested by the rank-sum test, and nonparametric data were tested by the Kruskal‒Wallis H test. The manual palpation and X-ray results showed that the fusion rate did not significantly differ between the CC1, CC5, CC9, CC13 and ABG groups. However, cavities appeared in CC9 and CC13 on the micro-CT image. The bone mass (BV/TV) of CC1, CC5, CC9 and CC13 was better than that of the ABG group, while almost no osteogenesis was observed in the NC group. Histologically, there was no obvious difference between the four groups except that the CC9 group and CC13 group had more fibrous tissues in the new bone. In conclusion, DMB with different cycling crushing times has no obvious difference in fusion rate of PLF, but it is slightly better than the ABG group.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9769509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of autologous platelet-rich plasma for long-term glucocorticoids caused chronic wound: a case report. 自体富血小板血浆治疗长期糖皮质激素所致慢性创伤的疗效：一例报告。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-03-10 DOI: 10.1007/s10561-023-10083-3

Ziang Zhang, Jie Long, Jian Geng, Wensen Xia

引用次数: 0

Manufacturing of CD34 + HPC-enriched, high-purity mononuclear cell products from umbilical cord blood. CD34的制造 + 来自脐带血的HPC富集的高纯度单核细胞产物。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-02-03 DOI: 10.1007/s10561-023-10070-8

Liam Wynn, Marnie Grace Wilson, Christopher Leonforte

{"title":"Manufacturing of CD34 + HPC-enriched, high-purity mononuclear cell products from umbilical cord blood.","authors":"Liam Wynn, Marnie Grace Wilson, Christopher Leonforte","doi":"10.1007/s10561-023-10070-8","DOIUrl":"10.1007/s10561-023-10070-8","url":null,"abstract":"The purpose of this study was to explore methods of selectively enriching CD34 + haematopoietic progenitor cells (HPC) in mononuclear cell (MNC) preparations, and to outline a procedure for cryopreservation and thawing of manufactured material. Density gradient centrifugation of umbilical cord blood was achieved using Ficoll-Paque™ media at 1.077 g/mL and 1.065 g/mL densities and Leucosep preparation tubes. Post-process samples were analysed for CD34 + and MNC content. Finally, MNCs were frozen down at a concentration of 8.5 × 106 cells/mL in CryoStor CS10 using an Asymptote VIAFreeze controlled rate freezer at a rate of - 2 °C per minute, then thawed and analysed for viability and recovery. Processing with 1.065 g/mL media selectively depleted non-HPC cell types, producing an approximately fourfold increase in CD34 + frequency (M ± 1SD = 1.4 ± 1.3%, P < 0.01) relative to the pre-process sample (M ± 1SD = 0.4 ± 0.3%), whereas 1.077 g/mL media produced only a twofold enrichment (0.7 ± 0.6, P < 0.01). This was not accompanied by any significant forfeit of CD34 + recovery (79 ± 32% vs. 78 ± 32% respectively; P = 0.87). The MNCs generated by the 1.065 g/mL procedure were of greater purity (96 ± 2%) than in the 1.077 g/mL procedure (80 ± 7%, P < 0.01). Post-thaw, MNC viability was 95 ± 1% and CD34 + viability was 98 ± 1%. Ultra-pure MNCs rich in CD34 + HPCs can be generated with a simple, inexpensive modification to Ficoll-Paque™ media. These products can be easily cryopreserved using a simple controlled rate freezing procedure.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9209607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insufficiency of collagenases in establishment of primary chondrocyte culture from cartilage of elderly patients receiving total joint replacement. 胶原酶在从接受全关节置换术的老年患者软骨中建立原代软骨细胞培养中的不足。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-05-03 DOI: 10.1007/s10561-023-10094-0

Jiamin Mao, Lexi Huang, Yiyang Ding, Xiaoyu Ma, Quanming Wang, Lei Ding

{"title":"Insufficiency of collagenases in establishment of primary chondrocyte culture from cartilage of elderly patients receiving total joint replacement.","authors":"Jiamin Mao, Lexi Huang, Yiyang Ding, Xiaoyu Ma, Quanming Wang, Lei Ding","doi":"10.1007/s10561-023-10094-0","DOIUrl":"10.1007/s10561-023-10094-0","url":null,"abstract":"Background Collagenases are frequently used in chondrocyte isolation from articular cartilage. However, the sufficiency of this enzyme in establishing primary human chondrocyte culture remains unknown. Methods Cartilage slices shaved from femoral head or tibial plateau of patients receiving total joint replacement surgery (16 hips, 8 knees) were subjected to 0.02% collagenase IA digestion for 16 h with (N = 19) or without (N = 5) the pre-treatment of 0.4% pronase E for 1.5 h. Chondrocyte yield and viability were compared between two groups. Chondrocyte phenotype was determined by the expression ratio of collagen type II to I. The morphology of cultured chondrocytes was monitored with a light microscope.Results Cartilage with pronase E pre-treatment yielded significantly higher chondrocytes than that without the pre-treatment (3,399 ± 1,637 cells/mg wet cartilage vs. 1,895 ± 688 cells/mg wet cartilage; P = 0.0067). Cell viability in the former group was also significantly higher than that in the latter (94% ± 2% vs. 86% ± 6%; P = 0.03). When cultured in monolayers, cells from cartilage with pronase E pre-treatment grew in a single plane showing rounded shape while cells from the other group grew in multi-planes and exhibited irregular shape. The mRNA expression ratio of collagen type II to I was 13.2 ± 7.5 in cells isolated from cartilage pre-treated with pronase E, indicating a typical chondrocyte phenotype. Conclusions Collagenase IA was not sufficient in establishing primary human chondrocyte culture. Cartilage must be treated with pronase E prior to collagenase IA application.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9411290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of MRI on stemness properties of Wharton's jelly-derived mesenchymal stem cells. MRI对Wharton氏胶状间充质干细胞干性的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10052-2

Mahnaz Tashakori, Fatemeh Asadi, Faezeh-Sadat Khorram, Azita Manshoori, Ali Hosseini-Chegeni, Fatemeh Mohseni Moghadam, Mahdieh Ahmadi Kamalabadi, Aliakbar Yousefi-Ahmadipour

{"title":"Effects of MRI on stemness properties of Wharton's jelly-derived mesenchymal stem cells.","authors":"Mahnaz Tashakori, Fatemeh Asadi, Faezeh-Sadat Khorram, Azita Manshoori, Ali Hosseini-Chegeni, Fatemeh Mohseni Moghadam, Mahdieh Ahmadi Kamalabadi, Aliakbar Yousefi-Ahmadipour","doi":"10.1007/s10561-022-10052-2","DOIUrl":"https://doi.org/10.1007/s10561-022-10052-2","url":null,"abstract":"Mesenchymal stem cells (MSCs), derived from various tissues, are served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton's jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. The aim of this study was to evaluate the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. Additionally, we assessed cell viability, growth kinetics and apoptosis of WJ-MSCs after MRI treatment. The quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) data showed that transcript levels of SOX-2, NANOG in MRI-treated WJ-MSCs were increased 32- and 213-fold, respectively. MTT assay was performed at 24, 48, and 72 h post-treatment and the viability was not significantly different between the two groups. The doubling time of the MRI group was markedly higher than the control group. In addition, the colony formation ability of WJ-MSCs after MRI treatment significantly increased. Furthermore, no change in apoptosis was seen before or after MRI treatment. Our results suggest that the use of MRI can improve the quality of MSCs and enhance the efficacy of mesenchymal stem cell-based therapies.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10106304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regenerative medicine improve neurodegenerative diseases. 再生医学改善神经退行性疾病。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10062-0

Seyedeh Sara Ashraf, Vahid Hosseinpour Sarmadi, Ghazaleh Larijani, Soheila Naderi Garahgheshlagh, Sara Ramezani, Soraya Moghadamifar, Seyedeh Lena Mohebi, Peiman Brouki Milan, Seyed Mohammad Amin Haramshahi, Nooshin Ahmadirad, Naser Amini

{"title":"Regenerative medicine improve neurodegenerative diseases.","authors":"Seyedeh Sara Ashraf, Vahid Hosseinpour Sarmadi, Ghazaleh Larijani, Soheila Naderi Garahgheshlagh, Sara Ramezani, Soraya Moghadamifar, Seyedeh Lena Mohebi, Peiman Brouki Milan, Seyed Mohammad Amin Haramshahi, Nooshin Ahmadirad, Naser Amini","doi":"10.1007/s10561-022-10062-0","DOIUrl":"https://doi.org/10.1007/s10561-022-10062-0","url":null,"abstract":"Regenerative medicine is a subdivision of medicine that improves methods to regrow, repair or replace unhealthy cells and tissues to return to normal function. Cell therapy, gene therapy, nanomedicine as choices used to cure neurodegenerative disease. Recently, studies related to the treatment of neurodegenerative disorders have been focused on stem cell therapy and Nano-drugs beyond other than regenerative medicine. Hence, by data from experimental models and clinical trials, we review the impact of stem cell therapy, gene therapy, and nanomedicine on the treatment of Alzheimer's disease (AD), Parkinson's disease (PD), and Amyotrophic lateral sclerosis (ALS). Indeed, improved knowledge and continued research on gene therapy and nanomedicine in treating Alzheimer's disease, Parkinson's disease, and Amyotrophic lateral sclerosis lead to advancements in effective and practical treatments for neurodegenerative diseases.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10106309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Vascular allografts for clinical application in Europe: assessment of 30 years of experience with vascular tissue banking in Brussels. 同种异体血管移植在欧洲的临床应用:评估30年的经验与血管组织银行在布鲁塞尔。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10063-z

Ramadan Jashari, Vanessa Bouzet, Maria-Josee Alcaraz Blanco, Alison Oleffe, Emilie Lecocq, Stefano Mastrobuoni

{"title":"Vascular allografts for clinical application in Europe: assessment of 30 years of experience with vascular tissue banking in Brussels.","authors":"Ramadan Jashari, Vanessa Bouzet, Maria-Josee Alcaraz Blanco, Alison Oleffe, Emilie Lecocq, Stefano Mastrobuoni","doi":"10.1007/s10561-022-10063-z","DOIUrl":"https://doi.org/10.1007/s10561-022-10063-z","url":null,"abstract":"Vascular tissue banking has been carried out in Brussels for over 30 years in compliance with EU and Swiss tissue banking regulations. A total of 2.765 vascular tissue donations were performed in Belgian, French, Netherlands and Suisse transplant centres: 547(20%), 1.013(37%) and 1.205(43%) during the first, second and third periods, respectively. 85% and 18% increase in donations during the second and third decades compared to previous one, were remarkable. Of the 7.066 evaluated vascular tissues, 2.407(227, 921 and 1.259) were discarded (34.1%), whereas 4.659(523, 1.861 and 2.275) accepted (65.9%) during the respective period. Of the 92 donated veins, 44(47.8%) were discarded and 48(52.2%) accepted. Allografts available for clinical application were stored in vapours of liquid nitrogen. A total of 4.636 allografts were delivered and transplanted for cases of infection (58%), critical limb ischaemia (16%) and congenital cardiac surgery (15%). Thirty veins were implanted. The progressive increases in donations of 20%, 37% and 43% and in transplantations of 20.8%, 34.6% and 45% during the first, second and third periods, respectively, were remarkable. Complications were reported after transplantation and these included acute rejection of two femoral arteries one month after transplantation. We conclude that the donation and transplantation of cryopreserved vascular allografts was stable with a progressive increase over time. Allografts were used predominantly for the treatment of infection, limb salvage for critical ischaemia and for neonates and infants with congenital cardiac malformation. Immune related rejection was observed. This should be a subject of future investigation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9809507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10106313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of ophthalmic surface anesthetic alcaine on the proliferation and apoptosis of human corneal endothelial cells through HIF-1α regulation. 眼表面麻醉碱通过HIF-1α调控对人角膜内皮细胞增殖和凋亡的影响。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2023-09-01 DOI: 10.1007/s10561-022-10057-x

Quan Wang, Zhao Zhang, Xuesong Gao

{"title":"Effects of ophthalmic surface anesthetic alcaine on the proliferation and apoptosis of human corneal endothelial cells through HIF-1α regulation.","authors":"Quan Wang, Zhao Zhang, Xuesong Gao","doi":"10.1007/s10561-022-10057-x","DOIUrl":"https://doi.org/10.1007/s10561-022-10057-x","url":null,"abstract":"The corneal endothelium is a monolayer, which mediates solute and water flux across the posterior corneal surface. Alcaine's main component proparacaine is paramount in human corneal endothelium (HCE) cell regulation. This study explored the mechanism of alcaine in regulating HCE cells. HCE cell morphology under gradient concentrations was observed by an optical microscope. Cell proliferation and viability were detected by MTT assay to determine the half inhibitory concentration (IC 50). Cell apoptosis rate, HIF-1α mRNA expression, and HIF-1α, p/t-JNK and Caspase-3 protein levels were detected by flow cytometry, RT-qPCR, and Western blot. After treatment with alcaine at 0.625-5 g/L concentration range for 24 h, HCE cells showed cytoplasmic vacuolation, cell shrinkage, separation from culture matrix, and eventual death. Alcaine treated-HCE cell proliferation was decreased in a dose-dependent manner. The IC 50 of alcaine was 1.26 g/L. After alcaine treatment, HCE cell apoptosis rate was promoted and HIF-1α levels in HCE cells were stimulated. Knockdown of HIF-1α partially annulled the effects of alcaine on inhibiting HCE cell proliferation and facilitating apoptosis. Alcaine might activate the JNK/caspase-3 pathway by increasing HIF-1α. The inhibition of the JNK/caspase-3 pathway partially abrogated the effects of alcaine on inhibiting HCE cell proliferation and promoting apoptosis. Alcaine might affect HCE cell proliferation and apoptosis by upregulating HIF-1α and activating the JNK/caspase-3 pathway.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10115795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0