Effect of hypoxia on the expression of microRNA in extracellular vesicles of human umbilical cord stem cells in vitro.

IF 1.4 4区医学 Q4 CELL BIOLOGY

Cell and Tissue Banking Pub Date : 2023-12-01 Epub Date: 2023-05-24 DOI:10.1007/s10561-023-10095-z

Huifen Zang, Zhaohui Wang, Qingqing Wu, Lei Shi, Ge Chen

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引用次数: 1

Abstract

Mesenchymal stem cells (MSCs) derived extracellular vesicles, which have been shown to possess therapeutic effects for many diseases. However, how hypoxic conditions would affect exosomal microRNA expression in human umbilical cord MSCs (hUC-MSCs) is currently not investigated. This study aims to investigate the potential function of in vitro microRNAs of hUC-MSC cultured under normoxic and hypoxic conditions. Extracellular vesicles secreted from hUC-MSCs cultured in normoxic (21% O₂) and hypoxic (5% O₂) conditions were collected for microRNA identification. Zeta View Laser Scattering and transmission electron microscopy were used to observe the size and morphology of extracellular vesicles. qRT-PCR was performed to measure the expression of related microRNAs. The Gene Ontology and KEGG pathway were used to predict the function of microRNAs. Finally, the effects of hypoxia on the expression of related mRNAs and cellular activity were examined. This study identified 35 upregulated and 8 downregulated microRNAs in the hypoxia group. We performed target genes analysis to explore the potential function of these microRNA upregulated in the hypoxia group. Significant enrichment of the cell proliferation, pluripotency of stem cells, MAPK, Wnt, and adherens junction pathways were observed in the GO and KEGG pathways. Under hypoxic conditions, the expression levels of 7 target genes were lower than that of the normal environment. In conclusion, this study demonstrated for the first time that microRNA expression in extracellular vesicles of human umbilical vein stem cells cultured under hypoxia is different from that under normal conditions, and these microRNAs may be markers for detecting hypoxia.

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缺氧对体外培养的人脐带干细胞胞外小泡中微小RNA表达的影响。

间充质干细胞衍生的细胞外小泡已被证明对许多疾病具有治疗作用。然而，缺氧条件如何影响人脐带间充质干细胞（hUC-MSCs）中外泌体微小RNA的表达目前尚未研究。本研究旨在研究在常氧和缺氧条件下培养的hUC MSC的体外微小RNA的潜在功能。收集在常氧（21%O2）和缺氧（5%O2）条件下培养的hUC-MSCs分泌的细胞外小泡用于microRNA鉴定。利用Zeta View激光散射和透射电子显微镜观察细胞外小泡的大小和形态。进行qRT-PCR以测量相关微小RNA的表达。利用基因本体论和KEGG通路预测微小RNA的功能。最后，研究了缺氧对相关信使核糖核酸表达和细胞活性的影响。这项研究在缺氧组中鉴定了35个上调和8个下调的微小RNA。我们进行了靶基因分析，以探索这些微小RNA在缺氧组中上调的潜在功能。在GO和KEGG途径中观察到细胞增殖、干细胞多能性、MAPK、Wnt和粘附分子连接途径的显著富集。在缺氧条件下，7个靶基因的表达水平低于正常环境。总之，本研究首次证明了缺氧培养的人脐静脉干细胞胞外小泡中微小RNA的表达与正常条件下不同，这些微小RNA可能是检测缺氧的标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell and Tissue Banking CELL BIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

3.10

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Cell and Tissue Banking provides a forum for disseminating information to scientists and clinicians involved in the banking and transplantation of cells and tissues. Cell and Tissue Banking is an international, peer-reviewed journal that publishes original papers in the following areas: basic research concerning general aspects of tissue banking such as quality assurance and control of banked cells/tissues, effects of preservation and sterilisation methods on cells/tissues, biotechnology, etc.; clinical applications of banked cells/tissues; standards of practice in procurement, processing, storage and distribution of cells/tissues; ethical issues; medico-legal issues.