Cell and Tissue Banking最新文献_第2页

Comparison of the structural integrity and quality of corneal endothelium stored in organ culture storage medium versus Eusol-C. 比较器官培养储存介质与 Eusol-C 中储存的角膜内皮的结构完整性和质量。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-31 DOI: 10.1007/s10561-024-10138-z

Qin Wang, Yonghuan Liu, Yang Gao, Ming Zhang, Lihong Yang, Jian Hu, Yi Ren, Qizhi Zhou, Meidong Zhu

{"title":"Comparison of the structural integrity and quality of corneal endothelium stored in organ culture storage medium versus Eusol-C.","authors":"Qin Wang, Yonghuan Liu, Yang Gao, Ming Zhang, Lihong Yang, Jian Hu, Yi Ren, Qizhi Zhou, Meidong Zhu","doi":"10.1007/s10561-024-10138-z","DOIUrl":"10.1007/s10561-024-10138-z","url":null,"abstract":"In this experimental study, we compared the structural integrity and cell quality of corneal endothelium stored in organ culture medium (OCS) and Eusol-C. The experiment included rabbit and human cornea experiments in vitro. Thirty rabbit corneas and thirty-two human corneas were collected and divided into two groups. All right corneas were allocated in experiment group and left corneas were placed in control group. The corneas in experimental group were stored in OCS at 34 °C, and the corneas in control group were stored in Eusol-C at 4 °C for 7, 14, 21, 28, and 35 days, respectively. Endothelial cell morphology, cell count, and trypan blue staining for viability were assessed before storage (Day 0) and at days 7, 14, 21, 28 and 35. The structural integrity of human corneal endothelial cell was analyzed using immunohistochemistry. The samples of storage solution for microbial culture were collected on the third day and at the end of storage. The results show that no bacterial and fungal infections were found in both groups. After 14 days of storage, the morphology of endothelial cell was better in the experimental group than in the control group. The endothelial cell stored in OCS were better than those stored in Eusol-C at the end of storage times, except human cornea 14 days storage group. The ZO-1 protein staining showed the typical polygonal morphology of endothelial cell stored in the OCS. Corneal endothelial cells stored in the OCS had better quality up to 28 days. It can be applied to Chinese eye banks as a method of corneal preservation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of nanographene oxide on adipose-derived stem cell cryopreservation. 纳米氧化物对脂肪干细胞冷冻的影响

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-06 DOI: 10.1007/s10561-024-10140-5

Zifei Li, Jun Qi, Su Fu, Jie Luan, Qian Wang

{"title":"Effects of nanographene oxide on adipose-derived stem cell cryopreservation.","authors":"Zifei Li, Jun Qi, Su Fu, Jie Luan, Qian Wang","doi":"10.1007/s10561-024-10140-5","DOIUrl":"10.1007/s10561-024-10140-5","url":null,"abstract":"Cryoinjury mitigation is key in cell cryopreservation. Here, we aimed to assess the effectiveness of nanographene oxide (nano-GO) for improving cryoprotectant agents (CPAs) in human adipose stem cell (hADSC) cryopreservation. For in vitro experiments, nano-GO (5 μg/mL) was added to the CPAs in the control, and passage (P) 2 hADSCs were collected and cryopreserved for around two weeks. We compared cytotoxicity, cell viability, immunophenotypes, proliferation, cell apoptosis, and tri-lineage differentiation. In vivo, studies used lipoaspirate to create non-enriched or hADSC-enriched fat tissues by combining it with PBS or hADSCs cryopreserved with the aforementioned CPAs. Each nude mouse received a 0.3 mL subcutaneous injection of the graft. At 12 weeks, the grafts were harvested. Histology, adipocyte-associated genes and protein, vascular density and angiogenic cytokines, macrophage infiltration, and inflammatory cytokines were analyzed. Nano-GO CPA contributed to increased cell viability, improved cell recovery, and lowered levels of early apoptosis. Nano GO at concentrations of 0.01-100 μg/mL caused no cytotoxicity to hADSCs. The absence of nano GOs in the intracellular compartments of the cells was confirmed by transmission electron microscopy. The fat grafts from the CPA-GO group showed more viable adipocytes and significantly increased angiogenesis compared to the PBS and CPA-C groups. Adding hADSCs from the CPA-GO group to the graft reduced macrophage infiltration and MCP-1 expression. Nano-GO plays an anti-apoptotic role in the cryopreservation of hADSCs, which could improve the survival of transplanted fat tissues, possibly via improved angiogenesis and lower inflammatory response in the transplanted adipose tissue.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of acellular scaffold loaded with Wharton's jelly-derived stem cells and mineral pitch on healing of burn model in rat. 含有沃顿果冻干细胞和矿物沥青的细胞支架对大鼠烧伤模型愈合的影响。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-13 DOI: 10.1007/s10561-024-10143-2

Linda Mohammadzadeh Boukani, Maryam Ezzati, Ahad Ferdowsi Khosroshahi, Raziyeh Kheirjou

{"title":"The effect of acellular scaffold loaded with Wharton's jelly-derived stem cells and mineral pitch on healing of burn model in rat.","authors":"Linda Mohammadzadeh Boukani, Maryam Ezzati, Ahad Ferdowsi Khosroshahi, Raziyeh Kheirjou","doi":"10.1007/s10561-024-10143-2","DOIUrl":"10.1007/s10561-024-10143-2","url":null,"abstract":"Severe burns often result in an exacerbated inflammatory response, which can contribute to further injury. This inflammatory response may lead to an increased risk of infection, multiple organ failure, and death. This study aimed to investigate the potential of reducing inflammation to enhance burn wound healing in rats using ovine's small intestinal submucosa as a carrier for Wharton's jelly mesenchymal stem cells (WJ-MSCs) and Mineral Pitch (MP). A rat burn model was developed, and the animals were divided into four groups: control group: burn, placebo group: scaffold-treated burn, cell experimental group: WJ-MSCs seeded scaffold-treated burn, and cell and MP experimental group: scaffolds loaded with WJ-MSCs and MP-treated burn. After treating the wounds in the relevant groups and sampling them on days 5, 14 and 21, histological and pathological parameters, and the expression of genes involved in angiogenesis and epithelialization were evaluated. The study results revealed several findings in the burn wounds. These included changes in mast cell populations, a decrease in inflammatory neutrophils and lymphocytes, an increase in fibroblasts and blood vessels, and upregulation of angiogenesis and epithelialization genes. These changes collectively contributed to enhanced wound healing in cell and MP experimental group compared to the other groups. The findings suggest that scaffolds loaded with Wharton's jelly-derived stem cells and MP can serve as engineered tools to modulate inflammatory conditions during the burn wound healing process. These interventions can improve burn wound management and promote better outcomes.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of Bowman's layer structure, function, and transplantation. 鲍曼层结构、功能和移植回顾。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-08-30 DOI: 10.1007/s10561-024-10148-x

Zuzana Sirolova, Martina Polachova, Alina-Dana Baxant, Pavel Studeny, Katarina Krivosheev, Magdalena Netukova

引用次数: 0

Background, establishment and initial experiences of the Danish cardiovascular homograft biobank 丹麦心血管同种移植生物库的背景、建立和初步经验

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-07-27 DOI: 10.1007/s10561-024-10137-0

Margrete Stenehjem, Dorte Kinggaard Holm, Lars Riber, Christian Nielsen, Sara Schødt Riber, Cengiz Akgül, Jes S. Lindholt

{"title":"Background, establishment and initial experiences of the Danish cardiovascular homograft biobank","authors":"Margrete Stenehjem, Dorte Kinggaard Holm, Lars Riber, Christian Nielsen, Sara Schødt Riber, Cengiz Akgül, Jes S. Lindholt","doi":"10.1007/s10561-024-10137-0","DOIUrl":"https://doi.org/10.1007/s10561-024-10137-0","url":null,"abstract":"Odense University Hospital is a major tertiary vascular hospital in Scandinavia, performing approx. 200 aortic repairs annually. This article presents the rationale behind this endeavor and the early outcomes of the initial implantation of locally processed homografts. All patients receiving a homograft were identified from the established homograft biobank database and their medical records were reviewed after obtaining consent. All surgeons in charge of homograft implantations were semi structured interviewed regarding the harvesting procedure, the tools for detecting available homografts, their quality and delivery. The National board of Health approved the biobank fulling the EU Directive of Tissues and Cells after 18 months of preparation. From May 6, 2021, to March 1, 2023, 26 patients had a homograft implantation, with 7 for mycotic aneurysms, 10 for aorto-iliac graft infection, 6 for infra-inguinal graft infection, and 3 for graft infection in thoracic aorta. Six (23%) were emergently performed. Two (7.7%) died within 30 days postoperatively, both following in situ replacement of an infected aortoiliac graft, corresponding to a 20% mortality in this subgroup. The incidence of reinfections was 19.2%; one each in the mycotic aneurysm group, the aortoiliac graft infection group, and the thoracic graft infection group. After 90 days, two patients were diagnosed with aorto-enteric fistula. All involved surgeons could easily identify available suitable homografts, and within 2 h have homografts of acceptable quality and requested dimensions. The establishment of the Danish Cardiovascular Homograft Biobank was straightforward and effectively serves cardiovascular procedures performed 24/7. Additionally, the initial experiences seem comparable to others experiences.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141773073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Approaches to reducing the toxic exposure hazard on the sheep population. 撤回说明：减少绵羊群体毒性暴露危害的方法。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 DOI: 10.1007/s10561-023-10113-0

Mynbay Umitzhanov, Asiya K Musaeva, Abdikalyk A Abishov, Toktar M Zhamansarin, Urzhan Zh Omarbekova, Sholpan Zh Turyspayeva, Sarsenbek T Siyabekov

引用次数: 0

Remaining microtia tissue as a source for 3D bioprinted elastic cartilage tissue constructs, potential use for surgical microtia reconstruction. 剩余的小体组织作为3D生物打印弹性软骨组织结构的来源，可能用于外科小体重建。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-12-01 DOI: 10.1007/s10561-023-10118-9

Cristina Velasquillo, Yaaziel Melgarejo-Ramírez, Julieta García-López, Claudia Gutiérrez-Gómez, Hugo Lecona, Maykel González-Torres, José Iván Sánchez-Betancourt, Clemente Ibarra, Sang Jin Lee, James J Yoo

{"title":"Remaining microtia tissue as a source for 3D bioprinted elastic cartilage tissue constructs, potential use for surgical microtia reconstruction.","authors":"Cristina Velasquillo, Yaaziel Melgarejo-Ramírez, Julieta García-López, Claudia Gutiérrez-Gómez, Hugo Lecona, Maykel González-Torres, José Iván Sánchez-Betancourt, Clemente Ibarra, Sang Jin Lee, James J Yoo","doi":"10.1007/s10561-023-10118-9","DOIUrl":"10.1007/s10561-023-10118-9","url":null,"abstract":"The absence of ears in children is a global problem. An implant made of costal cartilage is the standard procedure for ear reconstruction; however, side effects such as pneumothorax, loss of thoracic cage shape, and respiratory complications have been documented. Three-dimensional (3D) printing allows the generation of biocompatible scaffolds that mimic the shape, mechanical strength, and architecture of the native extracellular matrix necessary to promote new elastic cartilage formation. We report the potential use of a 3D-bioprinted poly-ε-caprolactone (3D-PCL) auricle-shaped framework seeded with remaining human microtia chondrocytes for the development of elastic cartilage for autologous microtia ear reconstruction. An in vivo assay of the neo-tissue formed revealed the generation of a 3D pinna-shaped neo-tissue, and confirmed the formation of elastic cartilage by the presence of type II collagen and elastin with histological features and a protein composition consistent with normal elastic cartilage. According to our results, a combination of 3D-PCL auricle frameworks and autologous microtia remnant tissue generates a suitable pinna structure for autologous ear reconstruction.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical and histological comparison of two suture configurations for soft tissue grafts: speedtrap™ versus krackow stitch. 用于软组织移植的两种缝合配置的生物力学和组织学比较：Speedtrap™ 与 Krackow 缝合。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2024-05-10 DOI: 10.1007/s10561-024-10133-4

Anell Olivos-Meza, Edgar Manuel Hernández-Espinoza, Víctor Manuel Domínguez-Hernández, Víctor Manuel Araujo-Monsalvo, Eréndira Georgina Estrada-Villaseñor, Raúl Pichardo-Bahena, Luis Ángel Murguía-González, Juan Diego Rodríguez-Reinoso, Jonatan de Jesus Hernández-León, Carlos Landa-Solis

{"title":"Biomechanical and histological comparison of two suture configurations for soft tissue grafts: speedtrap™ versus krackow stitch.","authors":"Anell Olivos-Meza, Edgar Manuel Hernández-Espinoza, Víctor Manuel Domínguez-Hernández, Víctor Manuel Araujo-Monsalvo, Eréndira Georgina Estrada-Villaseñor, Raúl Pichardo-Bahena, Luis Ángel Murguía-González, Juan Diego Rodríguez-Reinoso, Jonatan de Jesus Hernández-León, Carlos Landa-Solis","doi":"10.1007/s10561-024-10133-4","DOIUrl":"10.1007/s10561-024-10133-4","url":null,"abstract":"To compare 2 different graft preparation techniques to determine biomechanical strength and resultant tissue trauma evaluated by histology. Twelve common flexors of the finger's tendons were prepared with either tubulization (SpeedTrap™) or transtendon stiches (Orthocord™). The stiffness, resistance and energy at maximum load were tested for biomechanical assessment in both groups. After load testing, Samples were stained with hematoxylin and eosin (HE) to evaluate histological damage. We observe that the time to prepare tendons with SpeedTrap™ was 8.3 times faster (1:25 min) than traditional ones (15:02 min). In all cases, the mean values for SpeedTrap™ were higher in terms of strength, stiffness and energy at maximum load than for traditional suture but without significant difference (p > 0.05). The Krackow stitch produces greater structural damage to the collagen fibers while SpeedTrap™ maintains better organized arrangement of the fibers after tubulization preparation. With the results obtained, we can conclude that the tubulization technique allows faster graft preparation with less structural damage to the manipulated tissue without altering the biomechanical resistance provided by the transtendon suture technique.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture of cerebrospinal fluid-contacting neurons from neonatal mouse spinal cord. 从新生小鼠脊髓中培养脑脊液接触神经元。

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-06-27 DOI: 10.1007/s10561-023-10098-w

Liang Cao, Hui-Qian Zhang, Yu-Qi He, Ping-Jiang An, Lei-Luo Yang, Wei Tan, Gang Liu, Chun-Qing Wang, Xiao-Wei Dou, Qing Li

引用次数: 0

Effect of storage media on chondrocyte viability during cold storage of osteochondral dowels. 骨软骨榫冷藏过程中储存介质对软骨细胞活力的影响

IF 1.5 4区医学

Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2023-08-08 DOI: 10.1007/s10561-023-10104-1

Noor Abdelwahab, Mohammadhamed Shahsavari, Kezhou Wu, Leila Laouar, Tamara D Skene-Arnold, Janet A W Elliott, Nadr M Jomha

{"title":"Effect of storage media on chondrocyte viability during cold storage of osteochondral dowels.","authors":"Noor Abdelwahab, Mohammadhamed Shahsavari, Kezhou Wu, Leila Laouar, Tamara D Skene-Arnold, Janet A W Elliott, Nadr M Jomha","doi":"10.1007/s10561-023-10104-1","DOIUrl":"10.1007/s10561-023-10104-1","url":null,"abstract":"Osteochondral allograft transplantation is a successfully proven method to repair articular cartilage defects and prevent the degenerative effects of osteoarthritis. The number of osteochondral transplantations that can be performed each year is limited by availability of donor cartilage tissue and storage time constraints. Osteochondral transplantation success has been linked to high chondrocyte viability of the donor cartilage tissue at the time of implantation. Determining optimal storage conditions for donor cartilage is essential for tissue banks to safely provide quality cartilage tissue. In this study, we compared three tissue/cell media (DMEM/F12, RPMI-1640 and X-VIVO 10) for their ability to maintain chondrocyte viability during hypothermic storage for 28 days. Porcine osteochondral dowels were stored in each media for 28 days and cell viability was assessed every 7 days. Over the 28 day storage period, the chondrocyte viability of dowels stored in DMEM/F12, RPMI-1640, and X-VIVO 10 media all declined in a similar fashion. Our results show that all three media were equivalent in their ability to maintain cell viability of the cartilage tissue and provides rationale for the use of lower cost cell media (DMEM/F12 and RPMI-1640) for hypothermic storage of articular cartilage tissue.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9954389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0