Cell and Tissue Banking最新文献

{"title":"Scaffold Design: A Review of Material and Immune Modulation in Bone Tissue Engineering.","authors":"Mohamed Selim, Sleem A Farag, Gamal T Abdel-Jaber, Abdalla Abdal-Hay, Hamouda M Mousa","doi":"10.1007/s10561-026-10212-8","DOIUrl":"10.1007/s10561-026-10212-8","url":null,"abstract":"<p><p>With the growing population and increased life expectancy, there has been a significant rise in orthopedic fractures and pathologies, leading to a heightened demand for effective orthopedic solutions. Bone tissue engineering (BTE) has emerged as a promising approach, employing scaffolds to regenerate bone tissue. This review highlights that successful material design for BTE requires a comprehensive understanding of the composition, structure, and biomechanics of natural bone. It also necessitates the careful selection of biomimetic natural or tunable synthetic materials, including polymers, bioceramics, metals, and composites. Furthermore, optimizing the physical, mechanical, and chemical properties of scaffolds is crucial, as these factors influence cell adhesion, proliferation, and differentiation. Special attention is given to the interaction between scaffolds and the host immune system, including the strategic incorporation of bioactive molecules and immunoregulatory cells. This holistic approach aims to engineer scaffolds that not only meet structural and functional demands but also foster an immune-compatible environment to enhance bone regeneration effectively. Careful selection of effective immunomodulation strategies for 3D scaffolds is crucial for creating a supportive immune microenvironment without negative effects. Various approaches can enhance the immune response, including incorporating smart nanomaterials into the surface of scaffolds, which contribute to immunomodulation, angiogenesis, and osteogenesis. Using stem cells for regenerating damaged bone tissue also improves the scaffold's immune response. Moreover, ionic and molecular doping are effective methods used to enhance immune response of scaffold in (BET), where specific ions like magnesium, zinc, and silicon are added to improve bioactivity and immune modulation capabilities. Finally, Wnt/β-catenin signaling pathway can be activated by integrating lithium into the scaffold surface, as lithium has anti-inflammatory properties and promotes bone formation by activating these pathways.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146257463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of the microbiological quality control validation of corneal medium using a clinical C. acnes isolate.","authors":"Philipp Maximilian Maurer, Gefion Franke, Johannes K Knobloch","doi":"10.1007/s10561-026-10211-9","DOIUrl":"10.1007/s10561-026-10211-9","url":null,"abstract":"<p><p>The microbiological quality of corneal tissue for transplantation must be monitored in a validated procedure complying with the European Pharmacopoeia (Ph. Eur.). An automated culture system is used to detect test strains in the presence of the medium. The Ph. Eur. mentioned C. acnes ATCC 11827 strain has allegedly been isolated from skin. This strain is known to display variable growth in routine matrix validation. As the Ph. Eur. also opens the use of clinical isolates, this study analyzed the use of clinical isolates for matrix validation.We compared the time-to-detection (TTD) in absence and presence of cornea organ culture medium (cocm) of 24 C. acnes strains, preferring clinical isolates, in an automated culture system.In the first step clinical strains (n=23) were examined, thus 4 slow-growing (TTD 305,1-336 h), 5 intermediate-fast-growing (TTD 250,1-305 h), 5 fast-growing strains (TTD 164,3-250 h) and nine non-blood-culture-detectable strains (TTD>336 h) were identified. Seven strains showed a reproducible growth signal in cocm-absence. In presence of cocm, three strains (CA02, CA10, CA22) showed a reproducible growth signal, while the reference strain was not blood-culture-detectable (bcd) in four of five experiments. The strain with the preferred growth characteristics (DSM 117854) was tested according to the Ph. Eur. conditions and showed bcd growth in all measurements (n=5) in presence of medium, in contrast to the reference strain.The use of a clinical isolate of C. acnes could improve matrix validation of cocm quality control testing and thus the care of cornea-transplant-patients. C. acnes DSM 117854 provides an isolate that promises more reliable results in matrix validation.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"12"},"PeriodicalIF":2.0,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global research trends in allogenic bone and bone banking in orthopaedics: a 25-year bibliometric analysis (2000-2025).","authors":"Anil Regmi, Surakshya Baral","doi":"10.1007/s10561-026-10214-6","DOIUrl":"10.1007/s10561-026-10214-6","url":null,"abstract":"<p><p>Allogenic bone grafting and bone banking have become vital components of modern orthopaedic reconstruction, addressing bone loss following trauma, infection, tumor resection, and revision arthroplasty. Despite expanding clinical use, a comprehensive overview of global research productivity and collaboration in this domain has not been previously undertaken. A bibliometric analysis was conducted using the Scopus database (2000-2025) with defined search terms related to bone banking and allogenic bone grafting in orthopaedics. Data were analyzed using Scopus analytics and VOSviewer for publication trends, source impact, geographic distribution, authorship, funding patterns, and keyword co-occurrence networks. A total of 3497 documents were identified, showing steady publication growth. The majority were original research articles (79.2%). The United States led in publication output (37.7%), followed by China and Italy. Clinical Orthopaedics and Related Research and Spine were the most productive journals. Keyword mapping revealed core themes in revision arthroplasty, spinal fusion, limb reconstruction, and bone defect management. Global research on allogenic bone and bone banking demonstrates robust growth, interdisciplinary collaboration, and emerging regional contributions. Future efforts should emphasize standardization, outcome-based studies, and integration of biomaterials and regenerative technologies to enhance the safety and sustainability of bone banking worldwide.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"11"},"PeriodicalIF":2.0,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered exosomes for targeted bone regeneration: design, delivery, and functionalization.","authors":"Asrin Emami, Iman Menbari Oskouie","doi":"10.1007/s10561-026-10213-7","DOIUrl":"10.1007/s10561-026-10213-7","url":null,"abstract":"<p><p>Bone regeneration remains a major clinical challenge due to the limited healing capacity of large bone defects and the limitations of conventional grafting or cell-based therapies. Exosomes, nanosized extracellular vesicles secreted by diverse cell types, have emerged as promising cell-free mediators of osteogenesis, angiogenesis, and immune regulation. However, the therapeutic efficacy of native exosomes is constrained by low yield, rapid clearance, and limited targeting. Because effective bone regeneration is inherently multi-factorial-requiring biomechanical stability, vascularization, and an instructive ECM and cellular microenvironment-engineered exosomes should be regarded as enabling components within integrated regenerative systems rather than a standalone solution. Recent advances in engineered exosomes (EExos) have opened new frontiers in bone tissue regeneration by enabling precise design, biofunctionalization, and targeted delivery. Engineering strategies-ranging from genetic modification of donor cells to chemical conjugation, hybrid nanocarrier formation, and controlled cargo loading-have been employed to enhance the osteoinductive and osteoconductive potential of exosomes. Furthermore, incorporation of EExos into smart delivery systems, such as hydrogel scaffolds, 3D-printed matrices, and bone-targeting ligands, offers sustained release and localized therapeutic effects within the bone microenvironment. This review comprehensively summarizes the latest developments in the design, delivery, and functional optimization of EExos for bone regeneration. Mechanistic insights into their roles in promoting bone remodeling, angiogenesis, and immune modulation are discussed alongside current translational progress, manufacturing challenges, and regulatory considerations. Finally, emerging directions-such as AI-assisted exosome engineering, CRISPR-based programming, and bioprinting-integrated therapies-are highlighted as transformative pathways toward personalized and clinically translatable bone regenerative medicine.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"9"},"PeriodicalIF":2.0,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of donor-recipient gender mismatch on outcomes in Descemet membrane endothelial keratoplasty (DMEK).","authors":"Zoe Eckly, Andre M Trouvain, Maria Della Volpe-Waizel, Clara E Englisch, Peter Szurman, Berthold Seitz, Fabian Fries, Annekatrin Rickmann","doi":"10.1007/s10561-026-10210-w","DOIUrl":"10.1007/s10561-026-10210-w","url":null,"abstract":"<p><strong>Purpose: </strong>Previous studies have suggested that H-Y antigens may influence immunological outcomes in corneal transplantation. This study analyses the influence of donor-recipient gender mismatch in DMEK on postoperative outcomes.</p><p><strong>Methods: </strong>This single-center retrospective study included 2521 eyes (1712 patients) that underwent DMEK between January 2012 and December 2021. Patients were divided into four groups according to donor-to-recipient gender combinations (male-to-female, male-to-male, female-to-male, female-to-female) and were compared in terms of graft failure rate, visual acuity (VA), central corneal thickness (CCT), and endothelial cell count (ECC) preoperatively and at 1, 12 and 36 months postoperatively, as well as in terms of postoperative complications.</p><p><strong>Results: </strong>Of the 2521 cases, 1647 (65%) were sex-mismatched (male-to-female or female-to-male) and 874 (35%) were sex-matched (female-to-female or male-to-male). Intraoperative preparation time of the grafts (p = 0.78) and difficulties in the intraoperative handling (p = 0.43) were comparable in all groups. Re-bubbling or re-keratoplasty was required with comparable frequency (p = 0.84; p = 0.61 respectively). The occurence of primary or secondary graft failure was not statistically significant different between the groups (p = 0.57). No confirmed case of immunological graft rejection occurred. Functional postoperative outcomes for ECC (p = 0.18 after 1 month, p = 0.13 after 12 months, p = 0.27 after 36 months), CCT (p = 0.58 after 4-6 weeks, p = 0.82 after 12 months, p = 0.78 after 36 months) and VA (p = 0.64 after 4-6 weeks, p = 0.47 after 12 months, p = 0.39 after 36 months) were comparable in all groups at the follow-up.</p><p><strong>Conclusions: </strong>In this retrospective cohort, no documented immune graft rejection was observed, and sex mismatch did not influence intraoperative handling or postoperative interventions. However, prospective studies with standardized immunologic assessment are needed to clarify the role of donor-recipient sex mismatch in DMEK outcomes.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"10"},"PeriodicalIF":2.0,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimethyl fumarate as an immunomodulatory enhancer of adipose-derived mesenchymal stem cells: therapeutic implications.","authors":"Leila Abdullahi-Keivani, Mina Montazeri, Hossein Rahavi, Mohammad Taher Tahoori","doi":"10.1007/s10561-026-10209-3","DOIUrl":"10.1007/s10561-026-10209-3","url":null,"abstract":"<p><p>Adipose-drive stem cells (ASCs) are similar to Mesenchymal stem cells (MSCs), which are considered as multipotent progenitors that have capable of immunomodulation, self-renewal and differentiation into multiple cell lineages that can be used in cancer therapy or immune diseases. Dimethyl fumarate (DMF) is one of the important drugs with modulating function for treatment of various immune disease. The primary aim of this study was to investigate how dimethyl fumarate influences the immunomodulatory profile and functional properties of adipose‑derived mesenchymal stem cells under inflammatory conditions. Adipose-stimulating stem cells (ASCs) were isolated from the abdominal adipose tissue of ten patients via liposuction, and DMF was added after inducing inflammatory conditions with inflammatory cytokines such as IFN-γ and TNF-α. Cell culture and RT-PCR were performed to measure the expression of immunomodulatory factors such as Galectin-1/3, HGF, HO-1, CXCL-8, and IL-6. Flow cytometry for mesenchymal stem cell nature and confirmation was done. Fat and bone staining was performed to test the functional differentiation of these cells. ASCs treated with DMF at 0.01-100 μM concentration at 2 days showed the enhancement of CXCL-8 and IL-6 gene expression notably, whereas, HGF, and Galectin-1 reduced. Moreover, Galectin-3 and HO-1 depicted no significant difference compared to the control group. Further, Alizarin Red and Oil Red staining verified the change of MSCs toward adipose and osteogenic differentiation. ASCs are positive for markers including CD73+, CD90+, and CD105+ antigens by abs staining conjugated to fluorescence dye. Our study confirmed that ASCs combined with DMF have increasingly important roles through immune-modulatory properties by secreting mediators, which can suppress, improve, alter, or change the microenvironment of disease. Despite ongoing progress, the underlying stimulatory and regulatory mechanisms remain incompletely understood. The gap between pre‑clinical findings and clinical applications highlights the need for further investigation. Extensive research is still required to elucidate how therapeutic interventions achieve efficacy, ensure safety, and identify optimal strategies for targeting different organs in combination with other pharmacological agents.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"8"},"PeriodicalIF":2.0,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of corneal allograft rejection in post-penetrating keratoplasty patients: a retrospective analysis.","authors":"Miklós Ágoston Lukács, Mariann Fodor, László Módis","doi":"10.1007/s10561-025-10205-z","DOIUrl":"10.1007/s10561-025-10205-z","url":null,"abstract":"<p><p>To evaluate the treatment success and reversal rate of corneal allograft rejections in post-penetrating keratoplasty patients. Retrospective cohort study was performed in post-penetrating keratoplasty patients treated for corneal allograft rejection between September 2015 and August 2024 in a tertiary eye center in Debrecen, Hungary. Indication of keratoplasty, onset of the allograft rejection, best corrected visual acuity (BCVA), corneal transparency on slit lamp examination and applied treatment were recorded. In our institution's patient registry, 68 allograft rejection episodes were found. Before the rejection, all grafts were completely transparent. The rejection episodes occurred median 21 months after surgery. Diagnosis and treatment took place median 5 days after the onset of symptoms. Allograft rejection could be successfully reversed in 48 of the total 68 cases (70.6%). Treatment was adjusted individually and all patients received local treatment; 16 received only local and 52 received combined systemic and local corticosteroids resulting in a similar rejection reversal rate (13/16 vs. 35/52; p = 0.359). Comparing first (47) and repeat grafts (21), there were no significant differences between the treatment success rate (33/47 vs. 15/21; P = 1.000) and the frequency of combined local-plus-systemic treatment (34/47 vs. 18/21; p = 0.355). Before the rejection episodes, BCVA was 0.40 ± 0.30, which decreased following the rejection (0.28 ± 0.28; p < 0.001). This reduction in BCVA was observed in successfully treated cases as well (0.44 ± 0.28 vs. 0.38 ± 0.27; p = 0.008). Our dexamethasone-based local treatment demonstrated similar effectiveness in reversing corneal allograft rejections in patients underwent penetrating keratoplasty compared to data on prednisolone by other studies. However, systemic steroid augmentation might be needed more frequently when using topical dexamethasone.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"7"},"PeriodicalIF":2.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the surgical potential of Descemet's membrane: a standardized decellularization protocol.","authors":"Stefania D'Agostino, Elena Daniele, Diego Ponzin, Stefano Ferrari","doi":"10.1007/s10561-025-10207-x","DOIUrl":"10.1007/s10561-025-10207-x","url":null,"abstract":"<p><p>Descemet's membrane is the specialized extracellular matrix located between corneal stroma and endothelium. This basement membrane provides the biomechanical cues that sustain endothelium viability and function, making it an optimal cell scaffold. The present work describes a new decellularization protocol to remove the cellular components and obtain an acellular scaffold from Descemet's membrane. To induce cell lysis and eliminate all cytoplasmic and nuclear material, Descemet's membranes isolated from donor corneas were subjected to osmotic (hypotonic) shock. The efficiency of the decellularization process was evaluated by the quantification of total residual DNA and analysis by gel electrophoresis. Nuclei removal and extracellular matrix integrity after treatment were verified by histological analysis. In particular, the maintenance of collagen, glycoproteins, perlecan and elastin was analyzed in decellularized tissues compared to untreated controls. DNA quantification showed a 99% reduction of total DNA amount in decellularized Descemet's membranes compared to control ones, with only 28.9 ± 9.86 ng DNA/mg dry tissue residual. Furthermore, the agarose gel electrophoresis and the absence of visible nuclei after decellularization confirmed the efficiency of the process. Histological analyses showed that the composition of the extracellular matrix was not modified by the process. The decellularization protocol is effective in obtaining a Descemet's membrane that is depleted of donor DNA. Furthermore, the treatment preserves tissue matrix components. Descemet's membrane is already prestripped and provided by eye banks; therefore, a decellularized Descemet's membrane represents a valid candidate as a safe scaffold for intraocular surgery as, for example, in the treatment of refractory macular holes.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"5"},"PeriodicalIF":2.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a growth factor bioavailability enhanced allograft (GFBA) for bone regeneration.","authors":"Marie-Soleil R Smith, Sowmya Shivanna, Yakup Kohen, Shiva Naseri, Yasmin Mawani, Sean A F Peel","doi":"10.1007/s10561-025-10206-y","DOIUrl":"10.1007/s10561-025-10206-y","url":null,"abstract":"<p><p>Bone morphogenetic protein (BMP) stimulated osteoinduction is critical for bone regeneration. Human demineralized bone matrix (DBM) has been one of the most widely used bone graft substitutes, but its osteoinductive potential is weak and clinical effectiveness limited in part due to ineffective processing methods. Here, we describe a novel process designed to enhance allograft bioactivity by increasing the bioavailability of the native BMPs present within the matrix the product of which we call Natural Matrix Protein® (NMP®). BMP-7 release was quantified from NMP and DBM prepared from both bovine and human cortical bone. In both species, NMP significantly increased BMP-7 levels in acidic and physiologic extracts compared to DBM. NMP also demonstrated sustained release of BMP-7 and total protein for up to 12 weeks in simulated body fluid. Osteoinductive potential was evaluated in vitro using C2C12 cells and osteoinductivity in vivo in the athymic rat model. Direct treatment of cells with NMP in vitro produced a greater than tenfold increase in alkaline phosphatase activity at 40 mg/mL. In vivo, human NMP showed increased osteoinductivity compared to human DBM histologically, and the recovered NMP explants had significantly more mineralized bone and a higher bone volume fraction compared to DBM and to Infuse® Bone Graft (105 µg rhBMP-2 on an absorbable collagen sponge) as measured by microCT. These findings demonstrate that the novel NMP process reproducibly increases BMP-7 bioavailability, that NMP implants produce sustained BMP and protein release and have marked increase in osteoinductive activity.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"6"},"PeriodicalIF":2.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12827305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue engineering as a tool in a novel approach to the comprehensive treatment and management of a deeply and extensively burned patient: case report.","authors":"Katarzyna Czerny-Bednarczyk, Anna Słaboń, Karolina Ziółkowska, Agnieszka Klama-Baryła, Artur Wielgórecki, Marcin Gierek, Przemysław Strzelec, Wojciech Łabuś","doi":"10.1007/s10561-025-10203-1","DOIUrl":"10.1007/s10561-025-10203-1","url":null,"abstract":"<p><p>Deep and extensive thermal burns with concurrent inhalation injuries can be associated with a high mortality rate, especially among elderly patients. Injuries of this type require treatment in highly. specialized centers. Early excision and autografting are the standard of care for extensive, deep burns. To achieve a functionally and aesthetically satisfactory burn scar, allogeneic acellular dermal matrices (ADMs) can be used as co-grafts alongside autologous split-thickness skin grafts (STSGs). Additionally, the application of in vitro-cultured autologous keratinocytes and fibroblasts has been shown to accelerate burn wound healing. Allogeneic amnion transplantation can also be performed to promote healing at donor sites. This paper presents a case report of a 65 year-old patient with thermal burns covering 26% total body surface area (TBSA) with third-degree burns affecting the thorax, abdomen, back, right shoulder, right elbow, and right thigh, as well as airway involvement. The patient underwent multistage surgical treatment, including deep excision of necrotic tissues. The wound was treated using a combination of ADM, free STSG, in vitro-cultured skin cells, and local negative pressure wound therapy (NPWT). Allogeneic amnion grafts were applied to the donor sites, which were used multiple times after healing. Healing progress was monitored using laser speckle contrast analysis (LASCA). Additionally, scar viscoelasticity, transepidermal water loss, melanin content, epidermal thickness, and temperature were examined post-healing. Selected skin parameters were also assessed using high-frequency ultrasound. The patient was discharged on day 77, having spent 41 days in the surgical ward and 36 days in the rehabilitation ward, with fully healed wounds. It is important to note that rehabilitation began on the first day of hospitalization. Follow-up visits documented gradual improvement in the evaluated scar parameters.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 1","pages":"2"},"PeriodicalIF":2.0,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145586235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}