Cell and Tissue Banking最新文献

{"title":"Struggling with a cefazolin impregnation protocol of bone chips.","authors":"K Dendoncker, G Putzeys, T Nieuwenhuizen, P Voet, S Lambrecht, M Bertrand, H Valster, K Croes","doi":"10.1007/s10561-025-10164-5","DOIUrl":"10.1007/s10561-025-10164-5","url":null,"abstract":"<p><p>Antibiotics released locally through a carrier is a commonly used technique to prevent infection in orthopaedic procedures. Antibiotic-impregnated bone chips are an interesting carrier in bone reconstructive surgery. Cefazolin is a potentially interesting antibiotic given its proven efficiency in preventing surgical site infection when administered systemically. Preliminary in vitro studies with fresh frozen or processed bone chips impregnated with cefazolin solution showed rapid complete release within a few hours, questioning its potential for local infection prophylaxis. On the other hand, commercially available bone chips impregnated after purification using supercritical CO₂ have been shown to be an efficient carrier for the antibiotics vancomycin or tobramycin. With this in vitro study we wanted to investigate whether this specific type of processing protocol would improve the release pattern of cefazolin. In addition we investigated the impact of the timing of impregnation during the different steps of the processing protocol on the release of cefazolin.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 2","pages":"11"},"PeriodicalIF":1.4,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma-rich fibrin gel and adipose-derived allogeneic mesenchymal stem cells: innovation in the treatment of second-degree deep burn wound; characterization and in-vivo study.","authors":"Kimia Didehvar, Najmeh Kamali, Mehdi Haghshenas, Reyhaneh Yarmohammadi, Ghazaleh Larijani, Seyedeh Lena Mohebbi, Mohammad Amir Amirkhani, Naser Amini","doi":"10.1007/s10561-025-10158-3","DOIUrl":"10.1007/s10561-025-10158-3","url":null,"abstract":"<p><p>A biocompatible and readily available wound dressing for emergencies has been shown to be more cost-effective, while also reducing the risk of immune system-mediated reactions. In this project, we investigated the use of a fresh blood-derived matrix as a wound dressing, based on a 3D drug-loaded Plasma-rich Fibrin (PRF) scaffold, to support the transplantation of autologous stem cells for regenerating skin tissues lost due to burns. PRF scaffold was prepared from venous blood, and adipose tissue-derived stem cells (ADSCs) were isolated from the visceral fat tissue of rats. Following in vitro analysis, PRF gel and ADSCs were transplanted onto second-degree deep burn wounds on the backs of rats. Histopathological analysis and wound size measurements were conducted on days 5, 10, 15, and 21. The findings revealed that PRF gel, as a cyto-compatible scaffold with the potential for antibacterial drug release (sustained for up to 3 days, with up to 89.7% release), significantly enhanced the healing process in the treatment group. On day 15, a reduced wound size, mature skin cells, and well-organized, thicker collagen fibers were observed in the histopathology of the PRF-treated groups, which scored an average of (2.83  ±  0.04) out of 3 for overall histopathological parameters. The greatest wound contraction was seen in the scaffold-treated groups (5.32  ±   0.61 mm²), compared with the control group (7.96 ±  0.82 mm²) (p < 0.05). PRF scaffold and ADSCs have the potential to serve as an effective biological wound dressing for burn wounds, accelerating the healing process and offering an alternative to traditional skin grafting.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 2","pages":"10"},"PeriodicalIF":1.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using an acellular dermal matrix as a neuroprotective wrap-around for treating recurrent nerve entrapment syndromes: a proof of concept.","authors":"Till Wagner, Dietmar J O Ulrich","doi":"10.1007/s10561-025-10159-2","DOIUrl":"10.1007/s10561-025-10159-2","url":null,"abstract":"<p><p>Recurrent nerve entrapment syndrome is a well-known complication in peripheral nerve surgery that often leads to multiple reoperations and increases the risk of unfavorable outcomes due to scarring. In our outpatient clinic, we found two patients with recurrent nerve entrapment syndrome with significant symptoms such as complete sensory loss and chronic pain who were willing to undergo re-exploration of their entrapped nerves and cover them with a human acellular dermal matrix (ADM) as a protective shield against recurrence. Both patients had complete recovery of the nerve entrapment syndrome with satisfactory clinical results. The use of a human ADM appears to be a promising tool for recurrent nerve entrapment surgery without adding morbidity to the procedure.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"9"},"PeriodicalIF":1.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of human amniotic membrane, tilapia skin collagen, and Centella asiatica derived gel to treat burn wound in rat model.","authors":"Rabeya Akter, Md Liakat Hossain, Tusher- Al-Arafat, Polash Chandra Karmakar, Md Hasib Adnan, Farzana Diba, Nurul Karim, Naznin Akhtar, S M Asaduzzaman","doi":"10.1007/s10561-025-10157-4","DOIUrl":"10.1007/s10561-025-10157-4","url":null,"abstract":"<p><p>In the quest for an ideal wound healing material, human amniotic membrane (AM), tilapia skin collagen (TSC), and Centella asiatica (CA) have been studied separately for their healing potential. In this study, we formulated AM, TSC, and CA gel and studied their competency and wound healing efficacy in vivo. Gel was formulated using AM, TSC, CA, Carbopol 934, acrylic acid, glycerine, and triethanolamine and physicochemical properties e.g.,pH, water absorption, swelling variation, and nuclear magnetic resonance (NMR) spectroscopy were determined. Biological properties were determined by skin irritation study, brine shrimp lethality, andantibacterial activity. Wound healing potential was determined by applying gel to second-degree burnsin vivoby observing wound contracture, epithelialization period, and histological features. The gel was non-lethal to brine shrimp and had anti-bacterial activity and showed no edema or erythema after 7 days of topical application. After 21 days of treatment, the AM + TSC + CA group significantly (P < 0.001) accelerated wound contraction (95.75 ± 0.44%)whereasthenegative control had the lowest healing rate (40.32 ± 2.11%). Wound contraction rates of AM + TSC and TSC + CA groups were (68.12 ± 1.46%) and (82.52 ± 1.74%) respectively.Epithelialization period for AM + TSC + CA was only 22.7days whereas AM, TSC, CA, AM + TSC, AM + CA, TSC + CA, positive control, and negative control needed 29.3, 30.7, 31.3, 27.3, 26, 26.6, 25.3 and 36.6 days respectively. Histological analysis showed better healing potential for AM + TSC + CA regarding epidermal regeneration, blood vessel formation, and collagen deposition. The gel was biocompatible and in vivostudies with Wistar rats exhibited better wound healing capabilities than individual components of the gel alone.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"8"},"PeriodicalIF":1.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-temperature vacuum evaporation as a novel dehydration process for the long-term preservation of transplantable human corneal tissue.","authors":"Owen D McIntosh, Emily R Britchford, Lydia J Beeken, Andrew Hopkinson, Laura E Sidney","doi":"10.1007/s10561-024-10155-y","DOIUrl":"10.1007/s10561-024-10155-y","url":null,"abstract":"<p><p>Globally there is a shortage of available donor corneas with only 1 cornea available for every 70 needed. A large limitation to corneal transplant surgery is access to quality donor tissue due to inadequate eye donation services and infrastructure in many countries, compounded by the fact that there are few available long-term storage solutions for effectively preserving spare donor corneas collected in countries with a surplus. In this study, we describe a novel technology termed low-temperature vacuum evaporation (LTVE) that can effectively dry-preserve surplus donor corneal tissue, allowing it to be stored for approximately 5 years, shipped at room temperature, and stored on hospital shelves before rehydration prior to ophthalmic surgery. The dry-preserved corneas demonstrate equivalent biological characteristics to non-dried donor tissue, with the exception that epithelial and endothelial cells are removed and keratocytes are rendered non-viable and encapsulated within the preserved extracellular matrix. Structure and composition of the dried and rehydrated corneas remained identical to that of non-dried control corneas. Matrix-bound cytokines and growth factors were not affected by the drying and rehydration of the corneas. The ability to preserve human donor corneas using LTVE will have considerable impact on global corneal supply; utilisation of preserved corneas in lamellar keratoplasties, corneal perforations, ulcers, and tectonic support, will allow non-preserved donor tissue to be reserved for where it is truly required.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"7"},"PeriodicalIF":1.4,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11711135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose MSCs response to breast cancer cell-derived factors in conditioned media and extracts.","authors":"Fatemeh Sadeghian, Faezeh Kazemi, Ali Pirsadeghi, Fatemeh Asadi, Mahnaz Tashakori, Aliakbar Yousefi-Ahmadipour","doi":"10.1007/s10561-024-10156-x","DOIUrl":"10.1007/s10561-024-10156-x","url":null,"abstract":"<p><p>Interactions between MSCs and cancer cells are complex and multifaceted and have been shown to exhibit both pro-tumor and antitumor effects. This study investigated the effects of conditioned medium (CM) and cell extract (CE) from two different ERα statuses, MCF-7 and MDA-MB-231 breast cancer cell lines, on adipose-derived mesenchymal stem cells (ASCs). Findings showed that CM and CE increased cellular metabolic activity and viability of ASCs, upregulated angiogenic factors VEGF and HIF-1α, and cytokine TGF-β expression levels. However, CM and CE treatment did not significantly affect the clonogenicity of ASCs. In addition, apoptosis-related genes caspase-3 and 9 showed differential expression patterns among the treatment groups. The findings suggest that breast cancer cell-derived factors can modulate the behavior of ASCs, highlighting their potential as a therapeutic tool in breast cancer treatment and tissue regeneration. However, it is essential to consider the potential risks associated with CM and CE treatment on ASCs, as well as the potential recruitment of ASCs by cancer tumors and the risks associated with this recruitment. Further research is needed to elucidate these potential risks and benefits.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"6"},"PeriodicalIF":1.4,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of autologous chondrocyte transplantation for the treatment of osteoarthritis: a systematic review of clinical trials.","authors":"Mohamad Y Fares, Mohammad Daher, Peter Boufadel, Emil Haikal, Tarek Haj Shehade, Jonathan Koa, Adam Z Khan, Joseph A Abboud","doi":"10.1007/s10561-024-10154-z","DOIUrl":"10.1007/s10561-024-10154-z","url":null,"abstract":"<p><p>Tissue engineering and cartilage transplantation constitute an evolving field in the treatment of osteoarthritis, with therapeutic and clinical promise shown in autologous chondrocyte implantation. The aim of this systematic review is to explore current clinical trials that utilized autologous chondrocyte transplantation (ACT) and assess its efficacy in the treatment of osteoarthritis. PubMed, Ovid MEDLINE, and Google-Scholar (pages 1-20) were searched up until February 2023. Inclusion criteria consisted of clinical trials that involve autologous cartilage transplantation for the treatment of osteoarthritis. Clinical, imaging, arthroscopic, and histologic outcomes were assessed. A total of 15 clinical trials, involving 851 participants, were included in the study. All trials utilized ACT in the treatment of knee osteoarthritis through varying scaffolds: collagen-based (10 trials), polymer-based (2 trials), hyaluronic-acid based (2 trials), and spheroid technology (1 trial). Clinical improvement of patients undergoing ACT was noted in 14 trials; five showed superior clinical outcomes compared to the control group, while one showed inferiority compared to mesenchymal stem cells. Postoperative imaging was utilized to assess the degree of cartilage regeneration in 11 trials. Ten trials showed signs of cartilage recovery with ACT, four trials showed no difference, and two showed worse outcomes when compared to controls. Second-look-arthroscopy was performed in three trials, which reported varying degrees of improvement in cartilage regeneration. Histologic analysis was performed in four trials and generally showed promising results. While improved clinical outcomes were demonstrated, conflicting findings in postoperative outcome analysis raise questions about the unequivocal utility of ACT. Additional research with control groups, randomization, and appropriate blinding is required.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"5"},"PeriodicalIF":1.4,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a sterilization process for amniotic membrane allograft tissue using supercritical carbon dioxide and NovaKill.","authors":"Jennifer O'Connell, Komali Pentakota, Donny Villeareal, Jose Faz, Xiaoli Li, Anthony Trinh, Rachel Beddard, Scott Jones, Anand Srinivasan","doi":"10.1007/s10561-024-10152-1","DOIUrl":"10.1007/s10561-024-10152-1","url":null,"abstract":"<p><p>Amniotic membrane is arguably one of the most popular biological wound dressings on the market today. Various growth factors and cytokines inherent to amniotic membrane tissue have been recognized as key mediators in wound healing and tissue regeneration, giving the tissue its clinical utility. Sterilization methodologies using irradiation are recognized as the gold standard in the field and routinely used to prepare tissue allografts, including amniotic membrane for transplantation. However, irradiation is not always compatible in preserving the physical structure or biochemical factors of biological materials and can potentially result in detrimental effects to the critical quality attributes of allograft tissues. Alternatively, a novel sterilization technique involving supercritical carbon dioxide (SCCO₂) has been shown to have minimal effect on the inherent biophysical properties of sensitive biological tissues and tissue-derived products. At BioBridge Global, we have developed a process utilizing SCCO₂ technology for the sterilization of an amniotic membrane tissue allograft product. This process, first and foremost, meets industry standards for sterilization while simultaneously maintaining the biochemical composition of the tissue. Our results show that upon SCCO₂ sterilization, most of the growth factors tested were conserved, with many at quantities significantly greater than commercially available gamma and electron beam irradiated tissue. The SCCO₂-sterilized amniotic membrane allograft is unique in that it is designed to overcome limitations associated with traditional tissue sterilization methodologies, namely, the conservation of key biological factors inherent to native amniotic membrane tissue. It is anticipated that by retaining these biological factors, clinical outcomes associated with the use of SCCO₂-sterilized amniotic membrane will be improved.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"4"},"PeriodicalIF":1.4,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in preparation of acellular human dermis for tissue banking and transplantation.","authors":"Irit Stern, Valentina Barrera, Michael Randles, Paul Rooney","doi":"10.1007/s10561-024-10153-0","DOIUrl":"10.1007/s10561-024-10153-0","url":null,"abstract":"<p><p>Non-healing wounds cost the National Health Service over £5.6 billion annually in wound management. Skin allografts are used to treat non-healing wounds, ulcers and burns, offering the best protection against infection. In order to allow host cells to repopulate and to avoid immunogenicity, cell components are removed through decellularisation. Decellularisation of human dermis has so far been performed in NHS Blood and Transplant using a combination of two enzymes (RNase T1 and the recombinant human DNase Pulmozyme)®. This study aims at validating a new method to remove DNA from donated dermis via the use of a single enzyme, Benzonase, known for its effectiveness of DNA digestion. Skin samples were decellularised by removing the epidermis, lysing of dermal cells, removal of cellular fragments by a detergent wash and removal of nucleic acids by a nuclease incubation with either Benzonase or Pulmozyme + RNase T1. DNA quantification with PicoGreen, as well as histology on wax-embedded biopsies, stained with DAPI and haemotoxylin and eosin, were performed. In vitro toxicity test on human osteosarcoma immortalised cells and skin fibroblasts, and biomechanical (tensile) testing, were also performed. The effectiveness of DNA digestion with the new methodology was comparable to previous procedure. Mean DNA removal percentage following decellularisation with Pulmozyme + RNase was 99.9% (3.83 ng/mg). Mean DNA removal percentage with Benzonase was 99.8% (9.97 ng/mg). Histology staining showed complete decellularisation following either method. Benzonase was proven to be non-toxic to both cell lines used, and a one-way Anova test showed no significant difference in neither stress nor strain between acellular dermal matrix decellularised with either Benzonase or Pulmozyme + RNase T1. Benzonase was able to effectively decellularise dermis after prior removal of epidermis. It performed just as well as the combination of Pulmozyme + RNase T1, but represents significant advantages in terms of cost effectiveness, procurement and storage; Benzonase has been successfully used in the decellularisation of other tissues, thus would be better for Tissue Banking use. Switching to this combined DNase/RNase can have far-reaching consequences in the production of acellular human dermal matrix by NHSBT and in the treatment of patients requiring it.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"3"},"PeriodicalIF":1.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11628444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-mesenchymal stem cell-derived extracellular vesicles enhance angiogenesis and skin wound healing via bFGF-mediated VEGF expression.","authors":"Yonghu Ding, Mengsheng Song, Rong Huang, Weiting Chen","doi":"10.1007/s10561-024-10150-3","DOIUrl":"10.1007/s10561-024-10150-3","url":null,"abstract":"<p><p>This study aimed to investigate whether extracellular vesicles (EVs) derived from adipose-derived mesenchymal stem cells (ASCs) promote skin wound healing by delivering basic fibroblast growth factor (bFGF) to enhance vascular endothelial growth factor (VEGF) expression. ASCs were isolated and transfected with either a bFGF knockdown lentivirus (Lv-sh-bFGF) or a control lentivirus (Lv-sh-NC). EVs were extracted from ASCs cultures and characterized by transmission electron microscopy, nanoparticle tracking analysis, and Western blotting for surface markers. EVs were extracted from the conditioned mediums of ASCs and subjected to different treatments. These EVs or control treatments were injected at the wound edges. Wound healing was assessed using histological techniques, including H&E and Masson's trichrome staining to evaluate tissue regeneration, collagen organization, and immunohistochemistry for CD31 to quantify microvessel density. Protein expression of bFGF and VEGF was measured by Western blotting. ASC-derived EVs significantly promoted angiogenesis and improved skin wound healing. EVs encapsulating bFGF enhanced VEGF expression in the wound tissue, while knockdown of bFGF reduced both bFGF and VEGF expression, leading to delayed wound healing. Further knockdown of VEGF partially reversed the pro-angiogenic and wound-healing effects of bFGF-encapsulated EVs. This study demonstrates that ASC-derived EVs promoted skin wound repair by enhancing angiogenesis and accelerating tissue regeneration through the bFGF/VEGF axis. These findings highlight the therapeutic potential of ASCs-derived EVs in improving skin wound healing.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"2"},"PeriodicalIF":1.4,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}