Cell and Tissue Banking最新文献_第3页

A review of Bowman's layer structure, function, and transplantation. 鲍曼层结构、功能和移植回顾。

IF 16.4 4区医学

Cell and Tissue Banking Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI: 10.1007/s10561-024-10148-x

Zuzana Sirolova, Martina Polachova, Alina-Dana Baxant, Pavel Studeny, Katarina Krivosheev, Magdalena Netukova

引用次数: 0

A local, non-commercial tissue bank connected to an organ donor program can produce musculoskeletal allografts of uniform quality at very low costs - ten years' experience. 一个与器官捐献计划相连的非商业性地方组织库可以以非常低的成本生产出质量统一的肌肉骨骼同种异体移植物--十年的经验证明了这一点。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-11-23 DOI: 10.1007/s10561-024-10151-2

Helia Azkia, Lene H Harritshøj, Connie Nielsen, Niels Agerlin, Mette G Jensen, Jens G Hillingsø, Pia C Andersen, Michael R Krogsgaard

{"title":"A local, non-commercial tissue bank connected to an organ donor program can produce musculoskeletal allografts of uniform quality at very low costs - ten years' experience.","authors":"Helia Azkia, Lene H Harritshøj, Connie Nielsen, Niels Agerlin, Mette G Jensen, Jens G Hillingsø, Pia C Andersen, Michael R Krogsgaard","doi":"10.1007/s10561-024-10151-2","DOIUrl":"10.1007/s10561-024-10151-2","url":null,"abstract":"It is common practice that allograft tissues for knee multiligament reconstruction, meniscus transplantation, cartilage replacement and other advanced procedures are made available through commercial banks. The aim was to present the 10-year experience with a local, non-commercial tissue bank, established in 2014. The allograft bank was connected to an existing organ donor program. Following organ procurement, the musculoskeletal tissue is removed and stored immediately, fresh frozen to - 80 degrees Celsius (except hyaline cartilage, which is stored at 5 degrees Celsius). The donor is tested for contagious disease and the grafts for bacteria. When all results are negative, the grafts are released. When thawed before use a swap is cultured. Consecutive, prospectively collected data were analyzed. There were 31 donations, resulting in 1160 grafts. Only 40 grafts (3.4%) had a positive bacteria culture and were discarded. 552 recipients have been treated by use of these allografts. All grafts had negative bacterial cultures in swaps obtained before thawing, and there were no recorded transplantation related complications. The expenses for local grafts were 10-15% of the costs for grafts obtained from foreign banks. Through the local donation program, it was possible to establish a tissue bank with controlled quality grafts at minimal costs, minimizing the need for transportation of frozen grafts retrieved in other countries. Centers for specialized orthopedic surgery using allograft tissue can be self-providing and reduce costs by retrieving and handling allograft tissues locally.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"26 1","pages":"1"},"PeriodicalIF":1.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological evaluation of decellularization of freeze dried and chemically treated indigenously prepared bovine pericardium membrane. 对冷冻干燥和化学处理的本土制备的牛心包膜脱细胞进行组织学评估。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-23 DOI: 10.1007/s10561-024-10139-y

Chander Gupt, Arundeep Kaur Lamba, Farrukh Faraz, Shruti Tandon, Jeyaseelan Augustine, Archita Datta, Sachin Dhingra

{"title":"Histological evaluation of decellularization of freeze dried and chemically treated indigenously prepared bovine pericardium membrane.","authors":"Chander Gupt, Arundeep Kaur Lamba, Farrukh Faraz, Shruti Tandon, Jeyaseelan Augustine, Archita Datta, Sachin Dhingra","doi":"10.1007/s10561-024-10139-y","DOIUrl":"10.1007/s10561-024-10139-y","url":null,"abstract":"Decellularization is regarded as a xenogenic antigen-reduction technique because it effectively eliminates all cellular and nuclear components while mitigating any negative impact on the composition, biological functionality, and structural integrity of the remaining extracellular matrix. This study aimed to histologically evaluate native, freeze dried and chemically decellularized bovine pericardium membrane. Also, this study focused on preservation of extracellular matrix after decellularization. Bovine pericardium membrane was decellularized by freeze thaw cycle followed by freeze drying and 1% sodium dodecyl sulphate. Unprocessed pericardium was used as control. The effectiveness of Decellularization was assessed based on the reduction of histologically visible nuclei. Decellularization by freeze thaw cycle followed by freeze drying resulted in 17.84% reduction in nuclei content and decellularization by sodium dodecyl sulphate results in 92% reduction in nuclei content compare to control group. Picrosirius red staining for freeze dried group displayed loosely organised, thin collagen bundles that exhibit reddish-yellow birefringence and sodium dodecyl sulfate group revealed dense collagen bundles that are parallelly organised and compact, exhibiting reddish-yellow birefringence and showed good structural integrity. These results suggested that the sodium do decyl sulfate showed optimal decellularization results with better extracellular matrix preservation. It may be a suitable protocol for producing a suitable scaffold for periodontal tissue regeneration.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"773-784"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of cell therapy with adipose-derived stem cells in the treatment of acute rupture of the Achilles tendon in humans. 用脂肪来源干细胞进行细胞疗法治疗人类跟腱急性断裂的效果。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-21 DOI: 10.1007/s10561-024-10141-4

Maria Helena Costa Vieira, Laynna de Carvalho Schweich-Adami, Rodrigo Juliano Oliveira, Andréia Conceição Milan Brochado Antoniolli-Silva

引用次数: 0

Basic study on cryopreservation of rat calvarial osteoblasts with different cryoprotectants. 使用不同低温保护剂低温保存大鼠钙化成骨细胞的基础研究

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-07-08 DOI: 10.1007/s10561-024-10142-3

Xu Jiang, Tan Zhijian, Cao Min, Yu Rong, Tan Xinghui, Xin Gong

{"title":"Basic study on cryopreservation of rat calvarial osteoblasts with different cryoprotectants.","authors":"Xu Jiang, Tan Zhijian, Cao Min, Yu Rong, Tan Xinghui, Xin Gong","doi":"10.1007/s10561-024-10142-3","DOIUrl":"10.1007/s10561-024-10142-3","url":null,"abstract":"Cryopreservation is a method adopted for storage of autologous skulls. Herein, this current research sought to explore the effects of different cryoprotectants on the biological characteristics of rat calvarial osteoblasts after cryopreservation. Neonatal Sprague-Dawley rats were selected and their skull tissues were isolated. The skull tissues were allocated into the refrigerating-3M, refrigerating-6M, M199-3M, M199-6M, povidone iodine-3M, and povidone iodine-6M groups according to the usage of cryoprotectants and treatment time (month) and the fresh group. Osteoblasts were isolated from skull tissues in each group through digestion. The histomorphology of the skull was evaluated by H&E staining and cell morphology was observed by microscopy. The viability, proliferation, apoptosis, and osteogenic activity of osteoblasts were assessed by trypan blue staining, MTT, flow cytometry, and alkaline phosphatase (ALP) staining. The skull histomorphology and osteoblast morphology were similar between the fresh and refrigerating groups. Osteoblast viability was weakened after cryopreservation. The longer the refrigeration time, the lower the number of living cells and the higher the apoptosis rate. However, cryopreservation using different cryoprotectants did not evidently affect osteoblast proliferation and ALP activity. Different cryoprotectants show no apparent effect on the osteogenic activity of rat calvarial osteoblasts after cryopreservation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"755-764"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dose-dependent effects of gamma radiation sterilization on the collagen matrix of human cortical bone allograft and its influence on fatigue crack propagation resistance. 伽马射线灭菌对人皮质骨异体移植物胶原基质的剂量依赖效应及其对抗疲劳裂纹扩展的影响

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-15 DOI: 10.1007/s10561-024-10135-2

Dylan B Crocker, Thomas M Hering, Ozan Akkus, Megan E Oest, Clare M Rimnac

{"title":"Dose-dependent effects of gamma radiation sterilization on the collagen matrix of human cortical bone allograft and its influence on fatigue crack propagation resistance.","authors":"Dylan B Crocker, Thomas M Hering, Ozan Akkus, Megan E Oest, Clare M Rimnac","doi":"10.1007/s10561-024-10135-2","DOIUrl":"10.1007/s10561-024-10135-2","url":null,"abstract":"Fatigue crack propagation resistance and high-cycle S-N fatigue life of cortical bone allograft tissue are both negatively impacted in a radiation dose-dependent manner from 0 to 25 kGy. The standard radiation sterilization dose of 25-35 kGy has been shown to induce cleavage of collagen molecules into smaller peptides and accumulation of stable crosslinks within the collagen matrix, suggesting that these mechanisms may influence radiation-induced losses in cyclic fracture resistance. The objective of this study was to determine the radiation dose-dependency of collagen chain fragmentation and crosslink accumulation within the dose range of 0-25 kGy. Previously, cortical bone compact tension specimens from two donor femoral pairs were divided into four treatment groups (0 kGy, 10 kGy, 17.5 kGy, and 25 kGy) and underwent cyclic loading fatigue crack propagation testing. Following fatigue testing, collagen was isolated from one compact tension specimen in each treatment group from both donors. Radiation-induced collagen chain fragmentation was assessed using SDS-PAGE (n = 5), and accumulation of pentosidine, pyridinoline, and non-specific advanced glycation end products were assessed using a fluorometric assay (n = 4). Collagen chain fragmentation increased progressively in a dose-dependent manner (p < 0.001). Crosslink accumulation at all radiation dose levels increased relative to the 0 kGy control but did not demonstrate dose-dependency (p < 0.001). Taken together with our previous findings on fatigue crack propagation behavior, these data suggest that while collagen crosslink accumulation may contribute to reduced notched fatigue behavior with irradiation, dose-dependent losses in fatigue crack propagation resistance are mainly influenced by radiation-induced chain fragmentation.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"735-745"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jordanian nursing students' knowledge and attitudes of corneal donation. 约旦护理专业学生对角膜捐献的认识和态度。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-26 DOI: 10.1007/s10561-024-10145-0

Sanaa Rawashdeh, Mohammed ALBashtawy, Salam Bani Hani, Bayan ALBashtawy, Ahmad Ayed, Abdullah Alkhawaldeh, Asem Abdalrahim, Haitham Khatatbeh, Mohammad Suliman, Sa'd ALBashtawy, Islam Ali Oweidat, Rasmieh Al-Amer, Zaid ALBashtawy

{"title":"Jordanian nursing students' knowledge and attitudes of corneal donation.","authors":"Sanaa Rawashdeh, Mohammed ALBashtawy, Salam Bani Hani, Bayan ALBashtawy, Ahmad Ayed, Abdullah Alkhawaldeh, Asem Abdalrahim, Haitham Khatatbeh, Mohammad Suliman, Sa'd ALBashtawy, Islam Ali Oweidat, Rasmieh Al-Amer, Zaid ALBashtawy","doi":"10.1007/s10561-024-10145-0","DOIUrl":"10.1007/s10561-024-10145-0","url":null,"abstract":"A high success rate of corneal transplants is evident. However, there is still a lack of corneal grafts available to meet demand, largely because donors are reluctant to donate. Given their critical role in future healthcare teaching and advocacy. There has not been much research on Jordanian nursing students' perspectives on corneal donation, so it's critical to identify and eliminate any obstacles. This study aims to evaluate the knowledge and attitudes of Jordanian nursing students concerning corneal donation. A cross-sectional, descriptive design was used to recruit (n = 440) nursing students from four Jordanian universities. A self-reported questionnaire was used to obtain data on knowledge and attitudes regarding corneal donation. The average age of senior nursing students was (M = 23.07, SD = 3.63) years. Varying levels of understanding were revealed amongst university students toward corneal donation items. Generally, good attitude of nursing students toward corneal donation (M = 34.1, SD = 8.1). Weak positive relationship was found between total knowledge scores and age (r = 0.141, p = 0.003) while there is no significant relationship between age and total attitude score (r = 0.031, p = 0.552). Age was found to be a significant predictor (B = 0.01, Beta = 0.12, t = 2.07, p = 0.04). Also, the educational level of fathers is a significant positive predictor (Beta = 0.128, p = 0.008) for the total attitude scores among nursing students. Limited awareness of corneal donation, highlighting the need for focused educational interventions to improve their comprehension.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"861-872"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freezing does not influence the microarchitectural parameters of the microstructure of the freshly harvested femoral head bone. 冷冻不会影响新采集股骨头的微观结构参数。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-08-05 DOI: 10.1007/s10561-024-10147-y

Virginie Taillebot, Théo Krieger, Aurélien Maurel-Pantel, Youngji Kim, Matthieu Ollivier, Martine Pithioux

{"title":"Freezing does not influence the microarchitectural parameters of the microstructure of the freshly harvested femoral head bone.","authors":"Virginie Taillebot, Théo Krieger, Aurélien Maurel-Pantel, Youngji Kim, Matthieu Ollivier, Martine Pithioux","doi":"10.1007/s10561-024-10147-y","DOIUrl":"10.1007/s10561-024-10147-y","url":null,"abstract":"The femoral head is one of the most commonly used bones for allografts and biomechanical studies. However, there are few reports on the trabecular bone microarchitectural parameters of freshly harvested trabecular bones. To our knowledge, this is the first study to characterize the microstructure of femoral heads tested immediately after surgery and compare it with the microstructure obtained with conventional freezing. This study aims to investigate whether freezing at -80 °C for 6 weeks affects the trabecular microstructure of freshly harvested bone tissue. This study was divided into two groups: one with freshly harvested human femoral heads and the other with the same human femoral heads frozen at -80 °C for 6 weeks. Each femoral head was scanned using an X-ray microcomputed tomography scanner (µCT) to obtain the microarchitectural parameters, including the bone volume fraction (BV/TV), the mean trabecular thickness (Tb.th), the trabecular separation (Tb.sp), the degree of anisotropy (DA), and the connectivity density (Conn.D). There was no statistically significant difference between the fresh and the frozen groups for any of the parameters measured. This study shows that freezing at -80 °C for 6 weeks does not alter bone microstructure compared with freshly harvested femoral heads tested immediately after surgery.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"747-754"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141892988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical evaluation of a sheep tracheal scaffold. 绵羊气管支架的生物力学评估。

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI: 10.1007/s10561-024-10130-7

Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi

{"title":"Biomechanical evaluation of a sheep tracheal scaffold.","authors":"Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi","doi":"10.1007/s10561-024-10130-7","DOIUrl":"10.1007/s10561-024-10130-7","url":null,"abstract":"Tissue engineering is a set of techniques for producing or reconstructing tissue that primarily aims to restore or improve the function of tissues in the human body. The aim of the present study was to evaluate the mechanical and histological characteristics of decellularized tracheal scaffolds prepared in comparison with fresh trachea for use in tracheal repair. In order to prepare the scaffold, sheep's trachea was prepared and after cleaning the waste tissues, they were decellularized. Then decellularized scaffolds were evaluated histologically and laboratory and numerical study of the nonlinear mechanical behavior of tracheal tissue and scaffold and their comparison. Examining the results of histological evaluations showed that the decellularization of the scaffolds was completely done. These results were confirmed by hematoxylin-eosin staining. Also, the exact hyperelastic properties of tracheal tissue and scaffold were used in biomechanical models, and according to the presented results, the five-term Mooney-Rivlin strain energy density function became a suitable behavioral model for modeling the hyperelastic behavior of trachea and scaffold. In total, the results of this research showed that the scaffolds obtained from decellularization by preserving the main compositions of the desired tissue can be a suitable platform for investigating cell behaviors.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"765-772"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141075582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of Quercetin on wound healing in the human umbilical vein endothelial cells. 槲皮素对人脐带静脉内皮细胞伤口愈合的影响

IF 1.4 4区医学

Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-06-28 DOI: 10.1007/s10561-024-10144-1

Bahar Kartal, Ebru Alimogullari, Pınar Elçi, Tugba Fatsa, Sema Ören

{"title":"The effects of Quercetin on wound healing in the human umbilical vein endothelial cells.","authors":"Bahar Kartal, Ebru Alimogullari, Pınar Elçi, Tugba Fatsa, Sema Ören","doi":"10.1007/s10561-024-10144-1","DOIUrl":"10.1007/s10561-024-10144-1","url":null,"abstract":"An injury that affects the integrity of the skin, either inside or externally, is called a wound. Damaged tissue is repaired by a set of cellular and molecular mechanisms known as wound healing. Quercetin, a naturally occurring flavonoid, may hasten the healing of wounds. The study's objective was to investigate any potential impacts of quercetin on the wound-healing process. Human umbilical vein endothelial cells (HUVECs) were treated to varying dose ranges of quercetin (5-320 nM) for 24 and 48 h. Cultured cells were evaluated by using the MTT analysis, wound scratch assay and vascular tube formation. Furthermore the gene expression of VEGF and FGF were evaluated by qRT-PCR to determine the effects of quercetin on angiogenezis and wound repair. Positive effects of quercetin on cellular viability were demonstrated by the MTT experiment. In HUVECs quercetin promoted tube formation, migration, and proliferation while also averting wound breakage. Moreover, quercetin increased the expression of the FGF and VEGF genes, which aid in the healing of wounds in HUVECs. Quercetin may be bioactive molecule that successfully speeds up wound healing by regulating the vasculogenezis and healing cells.","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":" ","pages":"851-860"},"PeriodicalIF":1.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0