Cell and Tissue Banking最新文献

{"title":"Smart and stimuli-responsive hydrogels for controlled exosome delivery in bone tissue engineering: from passive carriers to intelligent therapeutic platforms.","authors":"Asrin Emami, Iman Menbari Oskouie","doi":"10.1007/s10561-026-10243-1","DOIUrl":"10.1007/s10561-026-10243-1","url":null,"abstract":"<p><p>Bone regeneration follows a tightly coordinated biological sequence in which inflammatory resolution, vascular formation, and matrix mineralization occur within distinct temporal windows. However, most current exosome delivery strategies primarily extend retention rather than synchronize therapeutic signaling with these dynamic healing stages. This mismatch between release kinetics and biological demand may limit the regenerative potential of extracellular vesicle therapies. This review proposes a biology-driven framework that reinterprets stimuli-responsive hydrogel systems according to their capacity to coordinate stage-specific exosome delivery throughout bone healing. Recent literature on hydrogel-based exosome delivery systems was critically analyzed and interpreted according to their ability to regulate therapeutic timing rather than stimulus type, encompassing endogenous-responsive, externally triggered, and hybrid platforms. Particular emphasis was placed on release kinetics, osteoimmunomodulation, angiogenic coupling, translational feasibility, and emerging adaptive delivery concepts. Current preclinical evidence suggests that stimuli-responsive hydrogels capable of synchronizing exosome release with successive stages of bone healing may improve inflammatory regulation, vascularization, and osteogenesis compared with passive delivery systems. However, these biological effects remain dependent on hydrogel formulation, exosome source, and experimental model. Multi-responsive and adaptive platforms appear particularly promising for achieving sequential therapeutic signaling, although most remain at the proof-of-concept or preclinical stage. Rather than functioning solely as sustained-release carriers, future exosome delivery systems are likely to evolve toward adaptive therapeutic platforms capable of integrating microenvironment sensing with programmable release behavior. The biology-driven framework proposed in this review provides a complementary perspective for the rational design of next-generation regenerative biomaterials while highlighting the key experimental and translational challenges that must be addressed before clinical implementation.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early clinical outcomes of keratoplasty using donor corneas preserved in sinasol (ZiSol) versus optisol-GS.","authors":"Kiana Hassanpour, Mohamadjavad Babaki, Mohammad Ali Javadi, Mozhgan Rezaei Kanavi","doi":"10.1007/s10561-026-10239-x","DOIUrl":"10.1007/s10561-026-10239-x","url":null,"abstract":"<p><p>To compare early postoperative outcomes of penetrating keratoplasty (PKP) and Descemet stripping automated endothelial keratoplasty (DSAEK) using donor corneas preserved in ZiSol (formerly Sinasol) versus Optisol-GS. Prospective, double-masked, randomized clinical trial. Ninety-seven eligible eyes undergoing PKP or DSAEK were randomized to receive corneas preserved in ZiSol or Optisol-GS at 4 °C. Preoperative assessments included uncorrected distance visual acuity (UDVA) and corneal edema grading. Postoperative visits at 1 week, 1, 3, and 6 months assessed UDVA, corrected distance visual acuity (CDVA), corneal edema grading, re-epithelialization, microbial keratitis, suture-related complications, and graft attachment (for DSAEK). The PKP cohort comprised 32 ZiSol and 20 Optisol-GS cases; the DSAEK cohort included 13 ZiSol and 23 Optisol-GS cases. At 6 months, mean CDVA was 0.52 ± 0.28 LogMAR (ZiSol) versus 0.41 ± 0.13 LogMAR (Optisol-GS) after PKP (P = 0.132) and 0.47 ± 0.26 versus 0.63 ± 0.40 LogMAR after DSAEK (P = 0.272). Final graft clarity was achieved in 93.3% (ZiSol) and 95.3% (Optisol-GS) of recipients. Complete re-epithelialization by day 7 occurred in 96.9% versus 100% of PKP cases (P > 0.999). Microbial keratitis occurred in 6.3% (ZiSol) and 0% (Optisol-GS) after PKP (P = 0.517). Complete graft attachment after DSAEK occurred in 100% (ZiSol) versus 87% (Optisol-GS) (P = 0.288). Early postoperative outcomes of PKP and DSAEK using ZiSol-preserved donor corneas were statistically comparable to those with Optisol-GS. ZiSol appears to be a safe, effective, and locally accessible alternative for mid-term hypothermic storage in resource-limited settings.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148419118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-derived mesenchymal stromal cells and their acellular derivatives in cutaneous wound healing and pathological scarring: a narrative review.","authors":"Nazanin Akbari, Banafsheh Heidari, Mehrangiz Totonchi","doi":"10.1007/s10561-026-10238-y","DOIUrl":"10.1007/s10561-026-10238-y","url":null,"abstract":"<p><p>Cutaneous wound healing is a tightly regulated biological process that restores tissue integrity following injury. Dysregulation of inflammation, fibroblast activity, extracellular matrix remodeling, and angiogenesis can result in delayed healing or pathological scarring, including hypertrophic scars and keloids. Conventional scar-management strategies, such as intralesional corticosteroids, surgical excision, radiotherapy, laser therapy, cryotherapy, silicone-based products, and pressure therapy, remain limited by variable efficacy, recurrence, adverse effects, and inconsistent long-term outcomes. Consequently, regenerative approaches based on adipose-derived mesenchymal stromal cells (ASCs) and ASC-derived acellular products have attracted increasing attention This narrative review synthesizes current evidence regarding ASC-based therapies and ASC-derived acellular products, including conditioned medium, soluble factors, ASC-derived nanovesicle therapy (extracellular vesicle preparations), and apoptotic extracellular vesicles, in cutaneous wound healing and pathological scar modulation. Particular emphasis is placed on scar-relevant mechanisms, including regulation of inflammation and macrophage polarization, modulation of fibroblast and myofibroblast activity, collagen remodeling, angiogenesis, re-epithelialization, transforming growth factor-β/Smad signaling, α-smooth muscle actin expression, and matrix metalloproteinase/tissue inhibitor of metalloproteinase balance. The review also positions ASC-derived products in relation to extracellular vesicles obtained from other sources, including placental, milk-derived, and plant-derived vesicles, and discusses emerging engineering strategies involving genetically modified ASCs, engineered extracellular vesicles, biomaterial-assisted delivery systems, and controlled-release platforms. Current evidence, which remains predominantly preclinical and methodologically heterogeneous, suggests that ASC-based therapies and ASC-derived acellular products may support tissue repair and attenuate pathways associated with pathological scar formation. However, substantial translational barriers remain, including donor-related variability, product heterogeneity, incomplete standardization of isolation and characterization methods, uncertain dose definitions, storage limitations, long-term safety concerns, and regulatory challenges. Well-designed clinical studies and standardized manufacturing frameworks are required before these approaches can be routinely integrated into wound-care and scar-management practice.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148419161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue donation in forensic cases: a retrospective study from Sweden.","authors":"Kajsa Kepplerus, Malin Pauli, Astrid Ohlin Åstrand, Jesper Greby, Ali-Reza Rezaie, Kristoffer Björkstrand, Brita Zilg, Nina Heldring","doi":"10.1007/s10561-026-10232-4","DOIUrl":"10.1007/s10561-026-10232-4","url":null,"abstract":"<p><p>Loss of a loved one under traumatic circumstances is challenging and often requires families to make difficult decisions. Forensic autopsy cases frequently involve sudden or traumatic deaths but do not preclude tissue donation or contact with next-of-kin. Many deceased individuals in these contexts are young and medically suitable for donation, making it important to understand when such donations are feasible to ultimately expand the pool of available tissues. This study investigates how next-of-kin interpret the deceased's presumed donation wishes based on manner of death, age, and sex. Using registry data from the Swedish National Board of Forensic Medicine, we analyzed demographics and consent outcomes was used to assess factors associated with next-of-kin consent. Of 1159 donation assessments, 691 cases involved next-of-kin contact. In cases without prior official donor declaration (556 individuals), consent was about twice as likely after natural death compared with accidents (OR 2.42) or suicide (OR 1.73). Among children aged 0-17, next-of-kin were markedly more likely to interpret boys' wishes as positive compared with girls (OR 7.59), while no sex differences were seen in adults. These findings show that tissue donation remains feasible in forensic cases and that both manner of death and age shape family interpretations, underscoring the need for tailored communication.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13346273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of PRF alone versus PRF plus biphasic calcium phosphate as a scaffold for gingival mesenchymal stem cells in induced vertical alveolar ridge defects.","authors":"Aya Hussien, Ashraf M Abu-Seida, Hala Abuel-Ela, Laila Rashed, Khaled Abdel-Ghaffar","doi":"10.1007/s10561-026-10236-0","DOIUrl":"10.1007/s10561-026-10236-0","url":null,"abstract":"<p><p>This research evaluated the effectiveness of platelet-rich fibrin (PRF) membrane alone versus the combined use of PRF membrane and biphasic calcium phosphate (BCP) block as a scaffold for gingival mensenchymal stem cells (GMSCs) in treatment of induced vertical alveolar ridge defects. Three standardized saddle-type defects were created in 10 adult mongrel dogs. Following a 4-week recuperation period, the defects were randomly assigned to one of three groups based on the treatment protocol: group I: defects augmented with only PRF membranes loaded with GMSCs, group II: treated with a combination of PRF membranes loaded with GMSCs and BCP block, and group III: treated with PRF membranes not loaded with GMSCs in addition to BCP block. After four months of augmentation procedures, histological and histochemical examinations were done. Two dogs were excluded from the study, resulting in a total sample size of 8 mongrel dogs. The data were statistically examined. Group II had the largest amount of bone growth inside the defect. However, evidence of bone maturation was more visible in group I. The difference in bone formation inside the defect between groups I and II was not statistically significant (P = 0.3395). However, there were significant differences between groups I and III and groups II and III (P < 0.0001). Histomorphometry revealed that group II had the highest mean area percentage of bone matrix, whereas group III had the lowest mean value. In conclusion, the combination of PRF loaded with MSCs and BCP block is a promising therapy option for vertical alveolar ridge defects in the future.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel-based strategies for dentin-pulp complex regeneration: a comprehensive review.","authors":"Roya Amani, Mina Rafiee, Erkan Karatas, Seher Hasanzade, Vahid Rahimkhoei, Ali Akbari","doi":"10.1007/s10561-026-10234-2","DOIUrl":"10.1007/s10561-026-10234-2","url":null,"abstract":"<p><p>The clinical management of dental caries encompasses a spectrum of therapeutic approaches, ranging from conservative restorative procedures to complex endodontic interventions. In cases involving pulp exposure or reversible pulpal injury, vital pulp therapy procedures are considered essential strategies for preserving pulp vitality and maintaining tooth function. This explains the focus of some recent works on developing tissue engineering (TE) approaches, particularly those involving dental stem or progenitor cells, to produce functional dental tissues. Hydrogel-based scaffolds, highly biocompatible three-dimensional (3D) bio/polymeric networks, are particularly attractive because of their ability to mimic the native extracellular matrix and thus serve as suitable scaffolds for tissue engineering, especially for dental pulp regeneration. Their versatility makes them a good candidate for a range of tissue engineering models, and hydrogel-based scaffolds are proving particularly valuable in regenerative medicine. This work reviews both natural and synthetic polymers, focusing on their structures and emerging uses in the regeneration of the dentin-pulp complex.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148350959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic properties of Mesenchymal stromal cells in burn wound infections caused by Pseudomonas aeruginosa.","authors":"Shabnam Razavi, Negar Narimisa, Davood Darban-Sarokhalil, Mohammad Esmaeil Amini","doi":"10.1007/s10561-026-10231-5","DOIUrl":"10.1007/s10561-026-10231-5","url":null,"abstract":"<p><p>Burn injuries cause significant damage to the skin and underlying tissues. This damage can lead to life-threatening complications due to the loss of the body's natural protective barrier. In severe cases, doctors often treat burns by surgically removing dead tissue and performing skin grafts, usually along with antibiotic therapy. However, burn wounds are very vulnerable to infection. Pseudomonas aeruginosa is one of the most common germs found in burn wound infections. Its growing resistance to antibiotics, especially in hospitals, creates a major challenge for treatment. Mesenchymal stromal cells (MSCs) have emerged as a promising option for managing burn wounds. Besides their ability to promote healing, MSCs can also regulate the immune response and fight infections. At the injury site, MSCs manage inflammation, encourage the growth of new blood vessels, and aid tissue repair through signaling molecules like growth factors, cytokines, and chemokines. Additionally, MSCs help the immune system by releasing antimicrobial peptides and proteins (AMPs) and by boosting the activity of immune cells such as phagocytes. This review elucidates the therapeutic potential of MSCs in managing burn wound infections caused by P. aeruginosa. By synthesizing findings from recent studies, it underscores the multifaceted role of MSCs in combating P. aeruginosa infections through their antibacterial, antibiofilm, and regenerative properties, offering a promising approach to address the challenges of antibiotic-resistant burn wounds.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148359553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of fresh versus short-term preserved stromal tissues obtained from DMEK in DALK surgery.","authors":"Ali Osman Gündogan, Yusuf Koçluk, Necip Kara, Refik Oltulu","doi":"10.1007/s10561-026-10233-3","DOIUrl":"10.1007/s10561-026-10233-3","url":null,"abstract":"<p><p>To evaluate the outcomes of deep anterior lamellar keratoplasty (DALK) using stromal tissues obtained during Descemet membrane endothelial keratoplasty (DMEK) and to compare the effectiveness of fresh versus three-day-stored grafts. This two-center retrospective study included 42 eyes of 42 patients who underwent DALK using DMEK-derived stromal tissues. Patients were divided into two groups: Group 1 (n = 23), in which fresh stromal tissue was used on the day of surgery, and Group 2 (n = 19), in which stromal tissue stored under cold-chain conditions-defined as transportation with the tissue temperature consistently maintained at approximately 4 °C-for three days was used. Preoperative and postoperative evaluations included best-corrected visual acuity (BCVA), intraocular pressure, slit-lamp and fundus examination, and corneal tomography (Pentacam HR; Oculus, Germany). Demographic data, intraoperative characteristics, postoperative graft transparency, and complications were compared between groups. The groups were similar regarding demographic features, surgical indications, anesthesia type, and suture technique (all p > 0.05). Graft transparency did not differ significantly between the groups on postoperative day 1 or at the last visit (p = 0.597, p = 0.890). Double anterior chamber formation occurred in 3 eyes (15.8%) in Group 1 and 6 eyes (26.1%) in Group 2 (p = 0.418). Other complications, including glaucoma, cataract, epithelial defects, and keratitis, occurred at similar rates (all p > 0.05). BCVA, keratometric, pachymetric, and densitometric parameters significantly improved postoperatively in both groups, with no intergroup difference (all p > 0.05). Stromal tissue obtained during DMEK is a safe and effective graft source for DALK, whether used fresh or after short-term storage. This technique optimizes donor cornea utilization and may help alleviate the global donor shortage.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seven-year experience (2018-2025) of a hospital-based cardiovascular tissue bank in Israel: operational insights and clinical impact.","authors":"Alina Levy, Helit Cohen, Michael Groysman, Alexandra Natanilov, Meytal Neeman-Azulay, Ehud Raanani, David Mishali, Rachel Kornhaber, Michelle Cleary, Ayelet Di Segni","doi":"10.1007/s10561-026-10230-6","DOIUrl":"10.1007/s10561-026-10230-6","url":null,"abstract":"<p><p>Cryopreserved human heart valves and cardiovascular tissues (homografts) are essential for the surgical treatment of congenital and acquired valvular diseases, particularly in pediatric patients, where prosthetic options are limited. To address the clinical demand for high-quality grafts in Israel, Sheba Medical Center, the largest tertiary care hospital in Israel, established a Good Manufacturing Practice (GMP) compliant Cardiovascular Tissue Bank within the public health system. This report presents an overview of the bank's operation within the Israeli public health system over seven years, covering donor selection criteria, tissue processing procedures, microbiological safety measures, cryopreservation and distribution, as well as protocol optimization and validation. Between 2018 and early 2025, the tissue bank successfully transitioned from basic sterile techniques to full GMP-grade cleanroom operations, implementing validated protocols for decontamination, packaging, and cryopreservation. During this period, 142 donor hearts were processed, yielding 390 cardiovascular tissues, of which 338 were approved for clinical use and distributed to cardiac surgery centers across Israel. This paper provides a comprehensive operational overview of the Sheba Cardiovascular Tissue Bank from 2018 to 2025, describing protocol refinement, tissue procurement, processing, preservation, regulatory compliance, quality control, and distribution. By combining clinical collaboration, rigorous quality control, and GMP-based infrastructure, the bank plays a pivotal role in ensuring the availability of high-quality cryopreserved homografts for Israeli patients, especially pediatric cases with complex congenital heart disease, and provides a sustainable model for hospital-based cardiovascular tissue banking.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13236731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clay/chitosan/poly(vinyl alcohol) nanofiber scaffold promotes differentiation of human dental pulp stem cells toward cholinergic and motor neuron subtypes.","authors":"Maryam Nazm Bojnordi, Hatef Ghasemi Hamidabadi, Saeid Bagheri-Mohammadi, Sara Saeednia, Kiana Kazemi","doi":"10.1007/s10561-026-10225-3","DOIUrl":"10.1007/s10561-026-10225-3","url":null,"abstract":"<p><p>This scientific research aimed to study the cholinergic and motor neuron-like cells differentiation potential of human dental pulp stem cells (hDPSCs) within a novel scaffold montmorillonite/poly (vinyl alcohol) (OMMT/PVA) nanofibrous mesh. The chitosan combined with different concentration of OMMT in PVA was synthesized and the main functional groups within OMMT/PVA nanofibers structure were then identified using ATR-FTIR. The probable cytotoxicity of the scaffolds was tested using MTT and LDH assays. hDPSCs underwent the differentiation process within the nanofibers with different concentrations of OMMT (1%, 3%, and 5%) in two separated phases. The differentiation capability of the cells was examined by analyzing the expression profiling of OCT-4, Nestin, GFAP, MBP, ChAT, MAP2, and Islet1, and Cresyl violet staining. Our findings proved that the scaffold could improve the viability and the proliferation rate of all experimental cell groups over the whole period of preinduction and induction phases. Moreover, the expression profile of genes indicated that the number of cells expressing neural-specific markers for those groups in the induction phase was substantially higher than that of the control group. The percentage of neural-specific markers positive cells increased in induced cells within 5% OMMT in PVA, which indicates that scaffolds provide hDPSCs with a suitable microenvironment for growing and differentiating toward desired neural cell lineages as well. Our finding shows that the OMMT/PVA fibrous scaffolds have a promising neuroprotective property and suitable potential for guiding of differentiation in the hDPSCs into motor neuron and cholinergic subtypes.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":"27 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}