The effects of Quercetin on wound healing in the human umbilical vein endothelial cells.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-01 Epub Date: 2024-06-28 DOI:10.1007/s10561-024-10144-1

Bahar Kartal, Ebru Alimogullari, Pınar Elçi, Tugba Fatsa, Sema Ören

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Abstract

An injury that affects the integrity of the skin, either inside or externally, is called a wound. Damaged tissue is repaired by a set of cellular and molecular mechanisms known as wound healing. Quercetin, a naturally occurring flavonoid, may hasten the healing of wounds. The study's objective was to investigate any potential impacts of quercetin on the wound-healing process. Human umbilical vein endothelial cells (HUVECs) were treated to varying dose ranges of quercetin (5-320 nM) for 24 and 48 h. Cultured cells were evaluated by using the MTT analysis, wound scratch assay and vascular tube formation. Furthermore the gene expression of VEGF and FGF were evaluated by qRT-PCR to determine the effects of quercetin on angiogenezis and wound repair. Positive effects of quercetin on cellular viability were demonstrated by the MTT experiment. In HUVECs quercetin promoted tube formation, migration, and proliferation while also averting wound breakage. Moreover, quercetin increased the expression of the FGF and VEGF genes, which aid in the healing of wounds in HUVECs. Quercetin may be bioactive molecule that successfully speeds up wound healing by regulating the vasculogenezis and healing cells.

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槲皮素对人脐带静脉内皮细胞伤口愈合的影响

影响皮肤内部或外部完整性的损伤称为伤口。受损组织通过一系列细胞和分子机制进行修复，这就是所谓的伤口愈合。槲皮素是一种天然类黄酮，可加速伤口愈合。这项研究的目的是调查槲皮素对伤口愈合过程的潜在影响。研究人员用不同剂量范围的槲皮素（5-320 nM）对人脐静脉内皮细胞（HUVECs）进行了 24 小时和 48 小时的处理。此外，还通过 qRT-PCR 评估了血管内皮生长因子（VEGF）和成纤维细胞生长因子（FGF）的基因表达，以确定槲皮素对血管生成和伤口修复的影响。MTT 实验证明了槲皮素对细胞活力的积极影响。在 HUVECs 中，槲皮素能促进管形成、迁移和增殖，同时还能避免伤口破裂。此外，槲皮素还能增加有助于 HUVECs 伤口愈合的 FGF 和 VEGF 基因的表达。槲皮素可能是一种生物活性分子，它能通过调节血管和愈合细胞成功地加速伤口愈合。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.