Histological evaluation of decellularization of freeze dried and chemically treated indigenously prepared bovine pericardium membrane.

IF 1.4 4区 医学 Q4 CELL BIOLOGY
Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-05-23 DOI:10.1007/s10561-024-10139-y
Chander Gupt, Arundeep Kaur Lamba, Farrukh Faraz, Shruti Tandon, Archita Datta, Sachin Dhingra
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Abstract

Decellularization is regarded as a xenogenic antigen-reduction technique because it effectively eliminates all cellular and nuclear components while mitigating any negative impact on the composition, biological functionality, and structural integrity of the remaining extracellular matrix. This study aimed to histologically evaluate native, freeze dried and chemically decellularized bovine pericardium membrane. Also, this study focused on preservation of extracellular matrix after decellularization. Bovine pericardium membrane was decellularized by freeze thaw cycle followed by freeze drying and 1% sodium dodecyl sulphate. Unprocessed pericardium was used as control. The effectiveness of Decellularization was assessed based on the reduction of histologically visible nuclei. Decellularization by freeze thaw cycle followed by freeze drying resulted in 17.84% reduction in nuclei content and decellularization by sodium dodecyl sulphate results in 92% reduction in nuclei content compare to control group. Picrosirius red staining for freeze dried group displayed loosely organised, thin collagen bundles that exhibit reddish-yellow birefringence and sodium dodecyl sulfate group revealed dense collagen bundles that are parallelly organised and compact, exhibiting reddish-yellow birefringence and showed good structural integrity. These results suggested that the sodium do decyl sulfate showed optimal decellularization results with better extracellular matrix preservation. It may be a suitable protocol for producing a suitable scaffold for periodontal tissue regeneration.

Abstract Image

对冷冻干燥和化学处理的本土制备的牛心包膜脱细胞进行组织学评估。
脱细胞被认为是一种异种抗原还原技术,因为它能有效消除所有细胞和核成分,同时减轻对剩余细胞外基质的成分、生物功能和结构完整性的负面影响。本研究旨在从组织学角度评估原生、冷冻干燥和化学脱细胞牛心包膜。此外,本研究还重点关注脱细胞后细胞外基质的保存情况。牛心包膜通过冻融循环脱细胞,然后冷冻干燥并用 1%十二烷基硫酸钠进行处理。未经处理的心包作为对照。根据组织学上可见细胞核的减少情况来评估脱细胞的效果。与对照组相比,冻融循环脱细胞后再冷冻干燥可使细胞核含量减少17.84%,十二烷基硫酸钠脱细胞可使细胞核含量减少92%。冷冻干燥组的毕赤染色显示组织松散、胶原纤维束稀疏并呈现红黄色双折射,而十二烷基硫酸钠组显示胶原纤维束致密,平行组织且紧密,呈现红黄色双折射,显示出良好的结构完整性。这些结果表明,十二烷基硫酸钠具有最佳的脱细胞效果,能更好地保存细胞外基质。它可能是制作牙周组织再生支架的合适方案。
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来源期刊
Cell and Tissue Banking
Cell and Tissue Banking CELL BIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
3.10
自引率
13.30%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Cell and Tissue Banking provides a forum for disseminating information to scientists and clinicians involved in the banking and transplantation of cells and tissues. Cell and Tissue Banking is an international, peer-reviewed journal that publishes original papers in the following areas: basic research concerning general aspects of tissue banking such as quality assurance and control of banked cells/tissues, effects of preservation and sterilisation methods on cells/tissues, biotechnology, etc.; clinical applications of banked cells/tissues; standards of practice in procurement, processing, storage and distribution of cells/tissues; ethical issues; medico-legal issues.
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