Basic study on cryopreservation of rat calvarial osteoblasts with different cryoprotectants.

IF 1.4 4区 医学 Q4 CELL BIOLOGY
Cell and Tissue Banking Pub Date : 2024-09-01 Epub Date: 2024-07-08 DOI:10.1007/s10561-024-10142-3
Xu Jiang, Tan Zhijian, Cao Min, Yu Rong, Tan Xinghui, Xin Gong
{"title":"Basic study on cryopreservation of rat calvarial osteoblasts with different cryoprotectants.","authors":"Xu Jiang, Tan Zhijian, Cao Min, Yu Rong, Tan Xinghui, Xin Gong","doi":"10.1007/s10561-024-10142-3","DOIUrl":null,"url":null,"abstract":"<p><p>Cryopreservation is a method adopted for storage of autologous skulls. Herein, this current research sought to explore the effects of different cryoprotectants on the biological characteristics of rat calvarial osteoblasts after cryopreservation. Neonatal Sprague-Dawley rats were selected and their skull tissues were isolated. The skull tissues were allocated into the refrigerating-3M, refrigerating-6M, M199-3M, M199-6M, povidone iodine-3M, and povidone iodine-6M groups according to the usage of cryoprotectants and treatment time (month) and the fresh group. Osteoblasts were isolated from skull tissues in each group through digestion. The histomorphology of the skull was evaluated by H&E staining and cell morphology was observed by microscopy. The viability, proliferation, apoptosis, and osteogenic activity of osteoblasts were assessed by trypan blue staining, MTT, flow cytometry, and alkaline phosphatase (ALP) staining. The skull histomorphology and osteoblast morphology were similar between the fresh and refrigerating groups. Osteoblast viability was weakened after cryopreservation. The longer the refrigeration time, the lower the number of living cells and the higher the apoptosis rate. However, cryopreservation using different cryoprotectants did not evidently affect osteoblast proliferation and ALP activity. Different cryoprotectants show no apparent effect on the osteogenic activity of rat calvarial osteoblasts after cryopreservation.</p>","PeriodicalId":9723,"journal":{"name":"Cell and Tissue Banking","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and Tissue Banking","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10561-024-10142-3","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/8 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Cryopreservation is a method adopted for storage of autologous skulls. Herein, this current research sought to explore the effects of different cryoprotectants on the biological characteristics of rat calvarial osteoblasts after cryopreservation. Neonatal Sprague-Dawley rats were selected and their skull tissues were isolated. The skull tissues were allocated into the refrigerating-3M, refrigerating-6M, M199-3M, M199-6M, povidone iodine-3M, and povidone iodine-6M groups according to the usage of cryoprotectants and treatment time (month) and the fresh group. Osteoblasts were isolated from skull tissues in each group through digestion. The histomorphology of the skull was evaluated by H&E staining and cell morphology was observed by microscopy. The viability, proliferation, apoptosis, and osteogenic activity of osteoblasts were assessed by trypan blue staining, MTT, flow cytometry, and alkaline phosphatase (ALP) staining. The skull histomorphology and osteoblast morphology were similar between the fresh and refrigerating groups. Osteoblast viability was weakened after cryopreservation. The longer the refrigeration time, the lower the number of living cells and the higher the apoptosis rate. However, cryopreservation using different cryoprotectants did not evidently affect osteoblast proliferation and ALP activity. Different cryoprotectants show no apparent effect on the osteogenic activity of rat calvarial osteoblasts after cryopreservation.

Abstract Image

使用不同低温保护剂低温保存大鼠钙化成骨细胞的基础研究
低温保存是一种用于储存自体头骨的方法。本研究旨在探讨不同冷冻保护剂对大鼠颅骨骨细胞冷冻保存后生物学特性的影响。研究人员选择了新生的 Sprague-Dawley 大鼠,并分离了它们的头骨组织。根据冷冻保护剂的使用和处理时间(月),将头骨组织分为冷冻-3M、冷冻-6M、M199-3M、M199-6M、聚维酮碘-3M、聚维酮碘-6M 组和新鲜组。各组头骨组织经消化后分离出成骨细胞。头骨的组织形态由 H&E 染色法评估,细胞形态由显微镜观察。通过胰蓝染色、MTT、流式细胞术和碱性磷酸酶(ALP)染色评估成骨细胞的活力、增殖、凋亡和成骨活性。新鲜组和冷藏组的头骨组织形态和成骨细胞形态相似。冷冻保存后,成骨细胞的活力减弱。冷藏时间越长,活细胞数量越少,凋亡率越高。然而,使用不同的冷冻保护剂进行冷冻并没有明显影响成骨细胞的增殖和 ALP 活性。不同的冷冻保护剂对大鼠犊骨成骨细胞冷冻保存后的成骨活性无明显影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Cell and Tissue Banking
Cell and Tissue Banking CELL BIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
3.10
自引率
13.30%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Cell and Tissue Banking provides a forum for disseminating information to scientists and clinicians involved in the banking and transplantation of cells and tissues. Cell and Tissue Banking is an international, peer-reviewed journal that publishes original papers in the following areas: basic research concerning general aspects of tissue banking such as quality assurance and control of banked cells/tissues, effects of preservation and sterilisation methods on cells/tissues, biotechnology, etc.; clinical applications of banked cells/tissues; standards of practice in procurement, processing, storage and distribution of cells/tissues; ethical issues; medico-legal issues.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信