Cell and Tissue Research最新文献_第3页

Involvement of vimentin- and BLBP-positive glial cells and their MMP expression in axonal regeneration after spinal cord transection in goldfish. 波形蛋白和 BLBP 阳性神经胶质细胞及其 MMP 表达参与金鱼脊髓横断后的轴突再生

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI: 10.1007/s00441-024-03907-5

Akihito Takeda, Minami Teshima, Kengo Funakoshi

{"title":"Involvement of vimentin- and BLBP-positive glial cells and their MMP expression in axonal regeneration after spinal cord transection in goldfish.","authors":"Akihito Takeda, Minami Teshima, Kengo Funakoshi","doi":"10.1007/s00441-024-03907-5","DOIUrl":"10.1007/s00441-024-03907-5","url":null,"abstract":"In goldfish, spinal cord injury triggers the formation of a fibrous scar at the injury site. Regenerating axons are able to penetrate the scar tissue, resulting in the recovery of motor function. Previous findings suggested that regenerating axons enter the scar through tubular structures surrounded by glial elements with laminin-positive basement membranes and that glial processes expressing glial fibrillary acidic protein (GFAP) are associated with axonal regeneration. How glia contribute to promoting axonal regeneration, however, is unknown. Here, we revealed that glial processes expressing vimentin or brain lipid-binding protein (BLBP) also enter the fibrous scar after spinal cord injury in goldfish. Vimentin-positive glial processes were more numerous than GFAP- or BLBP-positive glial processes in the scar tissue. Regenerating axons in the scar tissue were more closely associated with vimentin-positive glial processes than GFAP-positive glial processes. Vimentin-positive glial processes co-expressed matrix metalloproteinase (MMP)-14. Our findings suggest that vimentin-positive glial processes closely associate with regenerating axons through tubular structures entering the scar after spinal cord injury in goldfish. In intact spinal cord, ependymo-radial glial cell bodies express BLBP and their radial processes express vimentin, suggesting that vimentin-positive glial processes derive from migrating ependymo-radial glial cells. MMP-14 expressed in vimentin-positive glial cells and their processes might provide a beneficial environment for axonal regeneration.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"15-25"},"PeriodicalIF":3.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy accompanying the developmental process of male germline stem cells. 自噬伴随着男性生殖干细胞的发育过程。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-10-01 Epub Date: 2024-08-14 DOI: 10.1007/s00441-024-03910-w

Zhuofei Jiang, Liji Chen, Tao Wang, Jie Zhao, Shuxian Liu, Yating He, Liyun Wang, Hongfu Wu

{"title":"Autophagy accompanying the developmental process of male germline stem cells.","authors":"Zhuofei Jiang, Liji Chen, Tao Wang, Jie Zhao, Shuxian Liu, Yating He, Liyun Wang, Hongfu Wu","doi":"10.1007/s00441-024-03910-w","DOIUrl":"10.1007/s00441-024-03910-w","url":null,"abstract":"Germline stem cells are a crucial type of stem cell that can stably pass on genetic information to the next generation, providing the necessary foundation for the reproduction and survival of organisms. Male mammalian germline stem cells are unique cell types that include primordial germ cells and spermatogonial stem cells. They can differentiate into germ cells, such as sperm and eggs, thereby facilitating offspring reproduction. In addition, they continuously generate stem cells through self-renewal mechanisms to support the normal function of the reproductive system. Autophagy involves the use of lysosomes to degrade proteins and organelles that are regulated by relevant genes. This process plays an important role in maintaining the homeostasis of germline stem cells and the synthesis, degradation, and recycling of germline stem cell products. Recently, the developmental regulatory mechanism of germline stem cells has been further elucidated, and autophagy has been shown to be involved in the regulation of self-renewal and differentiation of germline stem cells. In this review, we introduce autophagy accompanying the development of germline stem cells, focusing on the autophagy process accompanying the development of male spermatogonial stem cells and the roles of related genes and proteins. We also briefly outline the effects of autophagy dysfunction on germline stem cells and reproduction.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"1-14"},"PeriodicalIF":3.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innexin expression and localization in the Drosophila antenna indicate gap junction or hemichannel involvement in antennal chemosensory sensilla. 果蝇触角中 Innexin 的表达和定位表明缝隙连接或半通道参与了触角化学感觉小体的活动。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-10-01 Epub Date: 2024-08-23 DOI: 10.1007/s00441-024-03909-3

Sinisa Prelic, Ian W Keesey, Sofia Lavista-Llanos, Bill S Hansson, Dieter Wicher

{"title":"Innexin expression and localization in the Drosophila antenna indicate gap junction or hemichannel involvement in antennal chemosensory sensilla.","authors":"Sinisa Prelic, Ian W Keesey, Sofia Lavista-Llanos, Bill S Hansson, Dieter Wicher","doi":"10.1007/s00441-024-03909-3","DOIUrl":"10.1007/s00441-024-03909-3","url":null,"abstract":"Odor detection in insects is largely mediated by structures on antennae called sensilla, which feature a strongly conserved architecture and repertoire of olfactory sensory neurons (OSNs) and various support cell types. In Drosophila, OSNs are tightly apposed to supporting cells, whose connection with neurons and functional roles in odor detection remain unclear. Coupling mechanisms between these neuronal and non-neuronal cell types have been suggested based on morphological observations, concomitant physiological activity during odor stimulation, and known interactions that occur in other chemosensory systems. For instance, it is not known whether cell-cell coupling via gap junctions between OSNs and neighboring cells exists, or whether hemichannels interconnect cellular and extracellular sensillum compartments. Here, we show that innexins, which form hemichannels and gap junctions in invertebrates, are abundantly expressed in adult drosophilid antennae. By surveying antennal transcriptomes and performing various immunohistochemical stainings in antennal tissues, we discover innexin-specific patterns of expression and localization, with a majority of innexins strongly localizing to glial and non-neuronal cells, likely support and epithelial cells. Finally, by injecting gap junction-permeable dye into a pre-identified sensillum, we observe no dye coupling between neuronal and non-neuronal cells. Together with evidence of non-neuronal innexin localization, we conclude that innexins likely do not conjoin neurons to support cells, but that junctions and hemichannels may instead couple support cells among each other or to their shared sensillum lymph to achieve synchronous activity. We discuss how coupling of sensillum microenvironments or compartments may potentially contribute to facilitate chemosensory functions of odor sensing and sensillum homeostasis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"35-62"},"PeriodicalIF":3.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11424723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A century of andrology in Cell & Tissue Research: looking back while moving forward 细胞与组织研究领域的一个世纪：回顾过去，展望未来

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-09-18 DOI: 10.1007/s00441-024-03916-4

Andreas Meinhardt, Peter Sutovsky

{"title":"A century of andrology in Cell & Tissue Research: looking back while moving forward","authors":"Andreas Meinhardt, Peter Sutovsky","doi":"10.1007/s00441-024-03916-4","DOIUrl":"https://doi.org/10.1007/s00441-024-03916-4","url":null,"abstract":"This article commemorates the 100th anniversary of the first issue of Cell & Tissue Research (CTR), the longest-running active journal dedicated to cell biology. Reflecting the significant contributions of spermatology and embryology to the early days of cell biology, the majority of articles in CTR’s inaugural issue centered on plant and animal sperm cells. A brief synopsis of these articles provides a launching point for revisiting 100 years of research on the male germ cells and fertility in humans and animals and offers a perspective on the current state and future directions of the andrology field. Early technological advances in light and electron microscopy enabled descriptive studies that ushered in the era of mechanistic, biochemistry-based inquiry focused on the understanding of physiological sperm processes such as sperm capacitation, acrosomal exocytosis, and sperm-egg interactions. In the last 20 years, progress in flow cytometry, cell imaging, and omics revealed new information on sperm proteome, transcriptome, metabolome, and overall phenome of fertile and infertile spermatozoa. Going back to the journal’s roots, recent advances in male germ cell isolation, transplantation, modification, and cryopreservation have been discussed on the pages of CTR. Newest trends such as gene editing and artificial intelligence/machine learning are now making inroads into andrological inquiry and assisted reproductive therapy of male infertility.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"17 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoneuroendocrine, circadian and seasonal systems: from photoneuroendocrinology to circadian biology and medicine 光神经内分泌、昼夜节律和季节系统：从光神经内分泌学到昼夜节律生物学和医学

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-09-12 DOI: 10.1007/s00441-024-03913-7

Horst-Werner Korf

{"title":"Photoneuroendocrine, circadian and seasonal systems: from photoneuroendocrinology to circadian biology and medicine","authors":"Horst-Werner Korf","doi":"10.1007/s00441-024-03913-7","DOIUrl":"https://doi.org/10.1007/s00441-024-03913-7","url":null,"abstract":"This contribution highlights the scientific development of two intertwined disciplines, photoneuroendocrinology and circadian biology. Photoneuroendocrinology has focused on nonvisual photoreceptors that translate light stimuli into neuroendocrine signals and serve rhythm entrainment. Nonvisual photoreceptors first described in the pineal complex and brain of nonmammalian species are luminance detectors. In the pineal, they control the formation of melatonin, the highly conserved hormone of darkness which is synthesized night by night. Pinealocytes endowed with both photoreceptive and neuroendocrine capacities function as “photoneuroendocrine cells.” In adult mammals, nonvisual photoreceptors controlling pineal melatonin biosynthesis and pupillary reflexes are absent from the pineal and brain and occur only in the inner layer of the retina. Encephalic photoreceptors regulate seasonal rhythms, such as the reproductive cycle. They are concentrated in circumventricular organs, the lateral septal organ and the paraventricular organ, and represent cerebrospinal fluid contacting neurons. Nonvisual photoreceptors employ different photopigments such as melanopsin, pinopsin, parapinopsin, neuropsin, and vertebrate ancient opsin. After identification of clock genes and molecular clockwork, circadian biology became cutting-edge research with a focus on rhythm generation. Molecular clockworks tick in every nucleated cell and, as shown in mammals, they drive the expression of more than 3000 genes and are of overall importance for regulation of cell proliferation and metabolism. The mammalian circadian system is hierarchically organized; the central rhythm generator is located in the suprachiasmatic nuclei which entrain peripheral circadian oscillators via multiple neuronal and neuroendocrine pathways. Disrupted molecular clockworks may cause various diseases, and investigations of this interplay will establish a new discipline: circadian medicine.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"106 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of animal-welfare compliant and sustainably sourced serum from pig slaughter blood. 从猪屠宰血液中提取符合动物福利标准且可持续的血清的潜力。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-09-01 Epub Date: 2024-07-11 DOI: 10.1007/s00441-024-03904-8

Olga Hahn, Kirsten Peters, Alexander Hartmann, Dirk Dannenberger, Claudia Kalbe

{"title":"Potential of animal-welfare compliant and sustainably sourced serum from pig slaughter blood.","authors":"Olga Hahn, Kirsten Peters, Alexander Hartmann, Dirk Dannenberger, Claudia Kalbe","doi":"10.1007/s00441-024-03904-8","DOIUrl":"10.1007/s00441-024-03904-8","url":null,"abstract":"The animal product most used as a stimulatory additive for cell cultivation is still fetal bovine serum (FBS). Besides the ethical concerns regarding serum collection, the main problems of FBS are batch-to-batch variability and the resulting risk of lower reproducibility, the differences between species, the presence of undefined/unknown components, and the risk of contamination. In contrast, pig blood, which is a by-product of slaughter, is a sufficiently available and sustainable resource with a high degree of standardization in terms of donor age, weight, and genetics. The variations in preparations from pig slaughter blood seem to be comparatively low, and consequently, batch effects might be much smaller, suggesting that the reproducibility of the research data obtained may be increased. Our pilot study aimed to investigate, as a proof of concept, whether adult human and porcine stem cells of different tissue origins proliferate and differentiate adequately when FBS is completely or partially replaced by porcine serum (PS). We could show that the human and porcine stem cells were vital and proliferated under partial and full PS supplementation. Furthermore, using PS, the two cell types studied showed tissue-specific differentiation (i.e., lipid vacuoles as a sign of adipogenic or myotubes as a sign of myogenic differentiation). In conclusion, the pig slaughter blood-derived serum has promising potential to be a replacement for FBS in adult stem cell cultures. Therefore, it could serve as a basis for the development of new cell culture supplements.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"205-214"},"PeriodicalIF":3.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the role of protein kinase A (PKA) activity in bovine oviductal epithelial cells: implications on apoptotic signaling pathways during the estrous cycle. 揭示牛输卵管上皮细胞中蛋白激酶 A (PKA) 活性的作用：对发情周期中凋亡信号通路的影响。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-09-01 Epub Date: 2024-08-06 DOI: 10.1007/s00441-024-03911-9

Juan Manuel Teijeiro

{"title":"Unveiling the role of protein kinase A (PKA) activity in bovine oviductal epithelial cells: implications on apoptotic signaling pathways during the estrous cycle.","authors":"Juan Manuel Teijeiro","doi":"10.1007/s00441-024-03911-9","DOIUrl":"10.1007/s00441-024-03911-9","url":null,"abstract":"The complex interactome crucial for successful pregnancy is constituted by the intricate network of endocrine and paracrine signaling pathways, involving gametes, embryos, and the female reproductive tract. Specifically, the oviduct exhibits distinct responses to gametes and early embryos during particular phases of the estrus cycle, a process tightly regulated by reproductive hormones. Moreover, these hormones play a pivotal role in orchestrating cyclical changes within oviductal epithelial cells. To unravel the molecular mechanisms underlying these dynamic changes, our study aimed to investigate the involvement of protein kinase A (PKA) in oviductal epithelial cells throughout the estrus cycle and in advanced pregnancy, extending our studies to oviductal epithelial cell in primary culture. By a combination of 2D-gel electrophoresis, Western blotting, and mass spectrometry, we identified 17 proteins exhibiting differential phosphorylation status mediated by PKA. Among these proteins, we successfully validated the phosphorylation status of heat shock 70 kDa protein (HSP70), aconitase 2 (ACO2), and lamin B1 (LMNB1). Our findings unequivocally demonstrate the dynamic regulation of PKA throughout the estrus cycle in oviductal epithelial cells. Also, analysis by bioinformatics tools suggest its pivotal role in mediating cyclical changes possibly through modulation of apoptotic pathways. This research sheds light on the intricate molecular mechanisms underlying reproductive processes, with implications for understanding fertility and reproductive health.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"275-285"},"PeriodicalIF":3.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141892987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined effect of naringin and adipose tissue-derived mesenchymal stem cell on cisplatin nephrotoxicity through Sirtuin1/Nrf-2/HO-1 signaling pathway: a promising nephroprotective candidate. 柚皮苷和脂肪组织间充质干细胞通过Sirtuin1/Nrf-2/HO-1信号通路对顺铂肾毒性的联合作用：一种有希望的肾保护候选物质。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-09-01 Epub Date: 2024-07-02 DOI: 10.1007/s00441-024-03902-w

Negin Amini, Fereshteh Nejaddehbashi, Mohammad Badavi, Vahid Bayati, Zahra Basir

{"title":"Combined effect of naringin and adipose tissue-derived mesenchymal stem cell on cisplatin nephrotoxicity through Sirtuin1/Nrf-2/HO-1 signaling pathway: a promising nephroprotective candidate.","authors":"Negin Amini, Fereshteh Nejaddehbashi, Mohammad Badavi, Vahid Bayati, Zahra Basir","doi":"10.1007/s00441-024-03902-w","DOIUrl":"10.1007/s00441-024-03902-w","url":null,"abstract":"Cisplatin nephrotoxicity is a well-known emergency clinical condition caused by oxidative stress and inflammation. Naringin (NAR) is considered an antioxidant agent with renoprotective effects capable of removing reactive oxygen species. Adipose tissue-derived mesenchymal stem cells (AD-MSCs) are reported to have anti-inflammatory and antioxidant properties. The present research examined the renoprotective effect of the combination of NAR and AD-MSCs as opposed to each one alone on cisplatin-induced nephrotoxicity through SIRT-1/Nrf-2/HO-1 pathway. This study included five groups (n = 8 each) of male Sprague-Dawley rats (200 - 220 g): sham, cisplatin: rats receiving cisplatin (6.5 mg/kg, i.p.) on the 4th day; NAR+cisplatin: rats pretreated with NAR (1 week, i.p.) + cisplatin on the 4th day; AD-MSCs: rats receiving AD-MSCs (1 × 106) by injection through the tail vein on the 5th day + cisplatin on the 4th day; and NAR+AD-MSCs+cisplatin. On the 8th day, the animals were anesthetized to obtain tissue and blood samples. Biochemical factors, inflammation, oxidative stress, and gene expression were explored. Cisplatin increased blood urea nitrogen, creatinine, inflammation, and oxidative stress. Moreover, mRNA expression of Sirtuin1, nuclear factor erythroid 2-related factor 2 (Nrf-2), and heme oxygenase-1 (HO-1) remarkably reduced. Furthermore, cisplatin led to a disturbance in kidney structure (glomerular atrophy, cell infiltrations, and tubular dysfunction) as confirmed by histology findings. However, NAR pretreatment, AD-MSC administration, or a combination of both significantly reversed these changes. Overall, when used together, NAR and AD-MSCs had stronger cisplatin-induced effects on kidney dysfunction by inhibiting inflammation, reducing oxidative stress, and increasing the Sirtuin1/Nrf-2/HO-1 pathway.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"193-204"},"PeriodicalIF":3.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-depth histological, lectin-histochemical, immunohistochemical and ultrastructural description of the olfactory rosettes and olfactory bulbs of turbot (Scophthalmus maximus). 大菱鲆嗅喙和嗅球的深入组织学、凝集素组织化学、免疫组织化学和超微结构描述。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-09-01 Epub Date: 2024-08-08 DOI: 10.1007/s00441-024-03906-6

Dorinda Torres, Paula R Villamayor, Albina Román, Pablo García, Paulino Martínez, Pablo Sanchez-Quinteiro

{"title":"In-depth histological, lectin-histochemical, immunohistochemical and ultrastructural description of the olfactory rosettes and olfactory bulbs of turbot (Scophthalmus maximus).","authors":"Dorinda Torres, Paula R Villamayor, Albina Román, Pablo García, Paulino Martínez, Pablo Sanchez-Quinteiro","doi":"10.1007/s00441-024-03906-6","DOIUrl":"10.1007/s00441-024-03906-6","url":null,"abstract":"Chemical communication through olfaction is crucial for fish behaviours, mediating in socio-sexual behaviours as reproduction. Turbot, a flatfish with significant aquaculture production, possesses a well-developed olfactory system from early developmental stages. After metamorphosis, flatfish acquire their characteristic bilateral asymmetry with an ocular side facing the open water column, housing the dorsal olfactory rosette, and a blind side in contact with the sea bottom where the ventral rosette is located. This study aimed to address the existing gap in specific histological, ultrastructural, lectin-histochemical and immunohistochemical studies of the turbot olfactory rosettes and olfactory bulbs. We examined microdissected olfactory organs of adult turbots and premetamorphic larvae by using routine histological staining techniques, and a wide array of lectins and primary antibodies against G-proteins and calcium-binding proteins. We observed no discernible structural variations in the olfactory epithelium between rosettes, except for the dorsal rosette being larger in size compared to the ventral rosette. Additionally, the use of transmission electron microscopy significantly improved the characterization of the adult olfactory epithelium, exhibiting high cell density, small cell size, and a wide diversity of cell types. Moreover, specific immunopositivity in sensory and non-sensory cells provided us of essential information regarding their olfactory roles. The results obtained significantly enriched the scarce morphological and neurochemical information available on the turbot olfactory system, revealing a highly complex olfactory epithelium with distinct features compared to other teleost species, especially with regard to olfactory cell distribution and immunolabelling patterns.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"215-239"},"PeriodicalIF":3.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal analysis of multi-scale cell structure in spheroid culture reveals hypertrophic chondrocyte differentiation. 球形培养物中多尺度细胞结构的时空分析揭示了肥大软骨细胞的分化。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-09-01 Epub Date: 2024-07-23 DOI: 10.1007/s00441-024-03905-7

Kosei Tomida, Jeonghyun Kim, Eijiro Maeda, Taiji Adachi, Takeo Matsumoto

{"title":"Spatiotemporal analysis of multi-scale cell structure in spheroid culture reveals hypertrophic chondrocyte differentiation.","authors":"Kosei Tomida, Jeonghyun Kim, Eijiro Maeda, Taiji Adachi, Takeo Matsumoto","doi":"10.1007/s00441-024-03905-7","DOIUrl":"10.1007/s00441-024-03905-7","url":null,"abstract":"3D cell culture has emerged as a promising approach to replicate the complex behaviors of cells within living organisms. This study aims to analyze spatiotemporal behavior of the morphological characteristics of cell structure at multiscale in 3D scaffold-free spheroids using chondrogenic progenitor ATDC5 cells. Over a 14-day culture period, it exhibited cell hypertrophy in the spheroids regarding cellular and nuclear size as well as changes in morphology. Moreover, biological analysis indicated a signification up-regulation of normal chondrocyte as well as hypertrophic chondrocyte markers, suggesting early hypertrophic chondrocyte differentiation. Cell nuclei underwent changes in volume, sphericity, and distribution in spheroid over time, indicating alterations in chromatin organization. The ratio of chromatin condensation volume to cell nuclear volume decreased as the cell nuclei enlarged, potentially signifying changes in chromatin state during hypertrophic chondrocyte differentiation. Our image analysis techniques in this present study enabled detailed morphological measurement of cell structure at multi-scale, which can be applied to various 3D culture models for in-depth investigation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"263-274"},"PeriodicalIF":3.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0