Cell and Tissue Research最新文献_第3页

Involvement of connexin 43 in myocardial ischemia-reperfusion injury. 连接蛋白43在心肌缺血再灌注损伤中的作用。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-06-19 DOI: 10.1007/s00441-025-03987-x

Marisol Ruiz-Meana, Javier Inserte, Marta Consegal, Antonio Rodríguez-Sinovas

{"title":"Involvement of connexin 43 in myocardial ischemia-reperfusion injury.","authors":"Marisol Ruiz-Meana, Javier Inserte, Marta Consegal, Antonio Rodríguez-Sinovas","doi":"10.1007/s00441-025-03987-x","DOIUrl":"10.1007/s00441-025-03987-x","url":null,"abstract":"Connexin 43 (Cx43) is the principal connexin isoform expressed in the ventricular myocardium, where it is critically involved in the pathophysiology of cardiac ischemia-reperfusion injury. Its functions in this pathological condition span at least three different fronts. First, Cx43-mediated gap junctional channels contribute to the spread of cellular damage during reperfusion, allowing the transfer of sodium ions between injured and surviving cardiomyocytes. Further, under ischemic conditions, unapposed Cx43 hemichannels exacerbate injury by promoting calcium overload, metabolite losses, and membrane potential instability. Additionally, recent evidence suggest that mitochondrial Cx43 influences oxidative stress by modulating reactive oxygen species generation through the regulation of reverse electron transfer (RET) at the mitochondrial electron transport chain. These detrimental roles of Cx43 in acute myocardial ischemia-reperfusion injury, together with its previously described involvement in ischemic preconditioning, emphasize the dual functionality and importance of Cx43 in the context of acute myocardial infarction. The scope of this review is to summarize the current knowledge on the different mechanisms by which Cx43 promotes cell damage during myocardial infarction, with special emphasis on the regulation of RET.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"297-309"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical relationships of huntingtin-associated protein 1 with choline acetyltransferase in the forebrain cholinergic nuclei of adult mice. 亨廷顿蛋白相关蛋白1与成年小鼠前脑胆碱能核胆碱乙酰转移酶的免疫组化关系。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-07-19 DOI: 10.1007/s00441-025-03996-w

Mirza Mienur Meher, Md Nabiul Islam, Akie Yanai, Marya Afrin, Mir Rubayet Jahan, Kanako Nozaki, Koh-Hei Masumoto, Koh Shinoda

{"title":"Immunohistochemical relationships of huntingtin-associated protein 1 with choline acetyltransferase in the forebrain cholinergic nuclei of adult mice.","authors":"Mirza Mienur Meher, Md Nabiul Islam, Akie Yanai, Marya Afrin, Mir Rubayet Jahan, Kanako Nozaki, Koh-Hei Masumoto, Koh Shinoda","doi":"10.1007/s00441-025-03996-w","DOIUrl":"10.1007/s00441-025-03996-w","url":null,"abstract":"Huntingtin-associated protein 1 (HAP1) is a core component of the stigmoid body (STB) and a neuroprotective interactor with the causative agents of several neurodegenerative diseases (NDs). The cholinergic system is often affected by NDs. Our previous studies suggest that cholinergic brainstem/spinal cord motoneurons are more vulnerable to neurodegeneration due to a lack of STB/HAP1 protectivity. The forebrain cholinergic nuclei are also major neurodegenerative/psychotic targets; however, the relationships of HAP1 with choline acetyltransferase (ChAT) have yet to be determined there. This study used western blotting and immunohistochemistry to evaluate the comparative distribution of HAP1 with ChAT and their immunohistochemical relationships in the adult mouse forebrain cholinergic nuclei. The results showed that HAP1-immunoreactive (ir) neurons were highly distributed in the basal forebrain cholinergic nuclei, including medial septal nucleus (MS), nucleus of vertical limb of the diagonal band of Broca (VDB), nucleus of horizontal limb of the diagonal band of Broca (HDB), and substantia innominata basal part (SIB). HAP1-ir neurons were sporadically scattered in the striatum. The significantly highest co-expression ratio of HAP1 with ChAT was observed in MS and VDB. In contrast, the ChAT-ir neurons never contained HAP1 in the caudate putamen of the striatum. These suggest that, due to having putative HAP1 protectivity, the cholinergic neurons in MS and VDB that are mainly projected to the hippocampal/parahippocampal regions might be protected to regulate social memory formation, emotion, and other psychological functions. Consequently, the lack of HAP1 protectivity might make cholinergic neurons in the striatum more prone to neurodegeneration in certain NDs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"237-263"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Male reproductive system in Aegla (Decapoda: Aeglidae) and its anatomic-histological and ultrastructural relationship with other anomuran crabs. 海蟹（十足目：海蟹科）雄性生殖系统及其与其他异形蟹的解剖组织学和超微结构关系。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-07-18 DOI: 10.1007/s00441-025-03993-z

Bárbara Paiva, Caio Santos Nogueira, Gustavo Monteiro Teixeira, Fernando José Zara

{"title":"Male reproductive system in Aegla (Decapoda: Aeglidae) and its anatomic-histological and ultrastructural relationship with other anomuran crabs.","authors":"Bárbara Paiva, Caio Santos Nogueira, Gustavo Monteiro Teixeira, Fernando José Zara","doi":"10.1007/s00441-025-03993-z","DOIUrl":"10.1007/s00441-025-03993-z","url":null,"abstract":"The male reproductive system (MRS) of decapods in the genus Aegla remains poorly understood from both histological and ultrastructural perspectives. This study provides a comparative description of the anatomy, histology, and ultrastructure of the MRS in multiple Aegla species, with the aim of exploring their phylogenetic relationships with representatives of the superfamilies Lomisoidea and Chirostyloidea. Anatomically, the MRS of Aegla is located in the cephalothorax and consists of a bilateral structure. The testes are connected by a central commissure and independently open into each vas deferens. The vas deferens is a translucent tube subdivided into proximal, medial, and distal regions. Across all regions, the seminal fluid contains few free spermatozoa, and spermatophores are absent. This fluid comprises two types of secretion: type I (basophilic) and type II (strongly basophilic), both composed mainly of proteins and acidic polysaccharides, with interspecific variation in acidic polysaccharide content. Aegla spermatozoa exhibit a standard organization, organized into two hemispheres-cytoplasmic and nuclear-and possess an acrosome vesicle with two concentric layers. Comparatively, their ultrastructure closely resembles that of Lomis hirta, whereas members of Chirostyloidea lack similar features, indicating an evolutionary divergence. These findings contribute important insights into the evolutionary history of Anomura, highlighting the absence of spermatophores in Aegla-a condition typical of this infraorder-and underscoring the similarity in spermatozoa ultrastructure between Aegla and Lomis, likely reflecting a shared ancestral trait.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"319-340"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue. 脂肪组织去泛素化酶CYLD失活小鼠的表型分析。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-07-02 DOI: 10.1007/s00441-025-03989-9

Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos

{"title":"Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue.","authors":"Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos","doi":"10.1007/s00441-025-03989-9","DOIUrl":"10.1007/s00441-025-03989-9","url":null,"abstract":"The cylindromatosis tumor suppressor (CYLD) is a deubiquitinating enzyme that has been implicated in lipid metabolism. More specifically, CYLD has been associated with lipid homeostasis in Drosophila melanogaster, and CYLD deficiency in mammals has been linked to dysregulation of lipid metabolism in the liver. Comprehensive tissue RNA expression analyses have revealed comparable levels of Cyld mRNA expression in the adipose tissue and liver, the organs that, together with skeletal muscle, primarily regulate lipid homeostasis. In the present study, the role of CYLD in mammalian adipose tissue homeostasis and function was investigated, utilizing a relevant conditional mouse model of CYLD inactivation that permits tissue-specific elimination of the catalytic domain of CYLD. Mutant mice displayed reduced weight-gain rate compared to controls when fed a normal or high-fat diet. Histological analysis of crown-like structures (CLS) indicated a reduced inflammatory response in the white adipose tissue of mutants. Our data collectively demonstrate that CYLD plays a pivotal role in regulating key metabolic parameters and modulating inflammatory responses within adipose tissue.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"311-317"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Aqp1 expression by osmotic balance in fenestrated endothelial cells of the posterior lobe of the pituitary. 垂体后叶开孔内皮细胞渗透平衡对Aqp1表达的调节。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-07-22 DOI: 10.1007/s00441-025-03995-x

Takashi Nakakura, Takeshi Suzuki

{"title":"Regulation of Aqp1 expression by osmotic balance in fenestrated endothelial cells of the posterior lobe of the pituitary.","authors":"Takashi Nakakura, Takeshi Suzuki","doi":"10.1007/s00441-025-03995-x","DOIUrl":"10.1007/s00441-025-03995-x","url":null,"abstract":"The posterior lobe (PL) of the vertebrate pituitary is richly vascularized with a dense network of fenestrated capillaries. In this study, we found that the expression of Aqp1, which encodes a plasma membrane-localized water channel protein, was significantly higher in endothelial fractions isolated from the rat PL than in those isolated from the anterior lobe (AL). Immunohistochemistry revealed aquaporin 1 (AQP1)-positive signals in fenestrated endothelial cells of the PL. Furthermore, immunoelectron microscopy demonstrated the presence of AQP1 signals on both the luminal and abluminal plasma membranes of these cells. AQP1 plays a pivotal role in facilitating water movement across the plasma membrane in response to changes in osmotic pressure on a cell. To investigate the effect of hyperosmolarity, we examined the expression levels of Aqp1 in the PL of water-deprived rats as well as in isolated endothelial cells of the PL cultured in a hyperosmotic medium supplemented with raffinose. Immunohistochemical analysis showed no changes in the proportion of AQP1-positive endothelial cells or in subcellular localization of AQP1 in cultured endothelial cells of the PL under hyperosmotic conditions. In contrast, analysis using quantitative real-time PCR revealed that hyperosmolar conditions significantly downregulated Aqp1 expression in cultured endothelial cells. These findings suggest that Aqp1expression in fenestrated capillaries in the PL is regulated by osmotic pressure of the interstitial fluid. Our results indicate that AQP1 is selectively expressed in fenestrated capillaries of the PL and plays a crucial role in maintaining water homeostasis in this region.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"265-274"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enteroepithelial stages of Toxoplasma gondii in the ileum of cats by serial block face scanning electron microscopy. 用连续块面扫描电镜观察猫回肠刚地弓形虫肠上皮分期。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-07-01 DOI: 10.1007/s00441-025-03988-w

Marcia Attias, Wanderley de Souza, Jitender P Dubey

引用次数: 0

Retraction Note: Guanabenz mitigates the neuropathological alterations and cell death in Alzheimer's disease. 备注：瓜纳苯可减轻阿尔茨海默病的神经病理改变和细胞死亡。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 DOI: 10.1007/s00441-025-03998-8

Abhishek Singh, Parul Gupta, Shubhangini Tiwari, Amit Mishra, Sarika Singh

引用次数: 0

Proteomic analysis of secreted proteins derived from amniotic fluid stem cells. 羊水干细胞分泌蛋白的蛋白质组学分析。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-06-07 DOI: 10.1007/s00441-025-03984-0

Tatsanee Phermthai, Puttachart Chuaynarong, Suparat Wichitwiengrat, Kamonpat Phermthai, Sittiruk Roytrakul, Thanuch Chitthira, Sasiprapa Thongbopit

{"title":"Proteomic analysis of secreted proteins derived from amniotic fluid stem cells.","authors":"Tatsanee Phermthai, Puttachart Chuaynarong, Suparat Wichitwiengrat, Kamonpat Phermthai, Sittiruk Roytrakul, Thanuch Chitthira, Sasiprapa Thongbopit","doi":"10.1007/s00441-025-03984-0","DOIUrl":"10.1007/s00441-025-03984-0","url":null,"abstract":"Mesenchymal stem cells (MSCs) show promising therapeutic effects due to the proteins they secrete. However, MSCs from different sources exhibit only 60% similarity of proteins they secrete, suggesting that unique proteins may offer distinct therapeutic properties based on their origin. Amniotic fluid-derived MSCs (AFSCs) are promising for treating degenerative diseases and are unique in providing sufficient cells for fetal therapies. Nevertheless, their proteomic profiles remain largely undefined. This study investigated the proteomic profiles of bioactive molecules secreted by AFSCs (AFSC-se) using liquid chromatography and mass spectrometry, along with bioinformatics tools for protein function analysis. We identified over 2000 proteins in the AFSC-se that are involved in various mechanisms supporting organ development and function. The top three proteins identified were associated with organelle fusion, forebrain morphogenesis, and response to parathyroid hormone. Our findings indicate that AFSC-se has the ability to inhibit inflammation and apoptosis, which corresponds to 7.8% of the identified proteins involved in pathways related to these therapeutic effects. Furthermore, we discovered that 20% of identified proteins are associated with brain functions including synaptogenesis, neurogenesis, and neuroprotection. In conclusion, the proteomic profile of AFSC-se indicates its potential therapeutic effects. The significant presence of neuro-related proteins in AFSC-se suggests that AFSC-se may be a promising candidate for treating neurological diseases. Our work addresses existing knowledge gaps in this field.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"275-286"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smarter stem cells: how AI is supercharging iPSC technology. 更智能的干细胞：人工智能如何强化iPSC技术。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-14 DOI: 10.1007/s00441-025-03999-7

Hany E Marei

{"title":"Smarter stem cells: how AI is supercharging iPSC technology.","authors":"Hany E Marei","doi":"10.1007/s00441-025-03999-7","DOIUrl":"https://doi.org/10.1007/s00441-025-03999-7","url":null,"abstract":"Integrated with artificial intelligence (AI), induced pluripotent stem cell (iPSC) technology could enhance disease modeling, cellular biology, regenerative medicine, and pharmaceutical development. AI has enhanced iPSC differentiation, cultural conditions, and speed of disease-specific model development. Furthermore, AI-based massive omics database analysis exposes hidden biological tendencies, enhancing customized treatment. Investigating new AI algorithms will enable one to solve problems, including interpretability and data quality, resulting from AI's interaction with iPSC technology. These advances fundamentally alter stem cell research and therapeutic applications, therefore facilitating the emergence of regenerative medicine and precision healthcare. AI has evolved in biomedical research into a transformational technology unique in great data analysis, predictive modeling, and automation capacity. AI integration increases the development of patient-specific cell types for disease modeling, pharmacological research, and regenerative medicine by substantially improving IPSC-based technologies. Emphasizing changes in disease models, alternative methodologies, and cellular reprogramming, this work examines current advancements in the use of AI in iPSC technology. The argument on significant obstacles and possibilities reveals how AI could alter the objectives of iPSC research and implementation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Folliculogenesis and follicular growth in the endangered fish Brycon opalinus, with emphasis on ovarian matrix remodeling. 濒危鱼类卵泡发生和卵泡生长，重点是卵巢基质重塑。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 Epub Date: 2025-05-19 DOI: 10.1007/s00441-025-03982-2

Camila Ferreira Sales, Bruna Aquino Araújo, Sarah Morais de Souza, Elizete Rizzo, Rafael Magno Costa Melo

{"title":"Folliculogenesis and follicular growth in the endangered fish Brycon opalinus, with emphasis on ovarian matrix remodeling.","authors":"Camila Ferreira Sales, Bruna Aquino Araújo, Sarah Morais de Souza, Elizete Rizzo, Rafael Magno Costa Melo","doi":"10.1007/s00441-025-03982-2","DOIUrl":"10.1007/s00441-025-03982-2","url":null,"abstract":"Ovarian development of fishes involves significant functional and structural changes during the process of mature egg formation. In this study, we report the folliculogenesis and follicular growth in the endangered species Brycon opalinus as well as the dynamics of the proteins Foxl2, Col4 and MMP-9 during ovarian maturation. Seventeen females were collected in the Doce River basin and their ovaries were subjected to histological techniques, electron microscopy, immunohistochemistry and Western blotting. In ovaries of B. opalinus, isolated oogonia and oogonia grouped in niches were identified, both surrounded by prefollicle cells. Primary growth initiated when the ovarian follicle was formed by the oocyte and follicle cells, in addition to the formation of zona radiata. Secondary growth was characterized by the incorporation of yolk into the oocyte and development of the zona radiata and follicle cells. Proportion of oogonia, perinucleolar follicles, and ovarian stroma was higher during resting stage, while previtellogenic and vitellogenic follicles were more frequent in the early and advanced maturation stages. Foxl2 was expressed mainly in prefollicle cells in oogonia and follicle cells in young follicles during reproductive resting. Col4 was expressed in basement membrane and extracellular matrix, primarily during resting and early maturation of ovaries. MMP-9 showed discrete labeling in ovarian matrix, but theca cells and zona radiata of secondary growth follicles, as well as granulocytes in matrix, showed significant immunolabeling during advanced maturation. We conclude that during ovarian development of B. opalinus there is intense participation and interaction of Foxl2, Col4 and MMP-9 with matrix components, somatic and germ cells for formation and follicular growth.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"167-179"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0