Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue.

IF 3.2 3区 生物学 Q3 CELL BIOLOGY
Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos
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引用次数: 0

Abstract

The cylindromatosis tumor suppressor (CYLD) is a deubiquitinating enzyme that has been implicated in lipid metabolism. More specifically, CYLD has been associated with lipid homeostasis in Drosophila melanogaster, and CYLD deficiency in mammals has been linked to dysregulation of lipid metabolism in the liver. Comprehensive tissue RNA expression analyses have revealed comparable levels of Cyld mRNA expression in the adipose tissue and liver, the organs that, together with skeletal muscle, primarily regulate lipid homeostasis. In the present study, the role of CYLD in mammalian adipose tissue homeostasis and function was investigated, utilizing a relevant conditional mouse model of CYLD inactivation that permits tissue-specific elimination of the catalytic domain of CYLD. Mutant mice displayed reduced weight-gain rate compared to controls when fed a normal or high-fat diet. Histological analysis of crown-like structures (CLS) indicated a reduced inflammatory response in the white adipose tissue of mutants. Our data collectively demonstrate that CYLD plays a pivotal role in regulating key metabolic parameters and modulating inflammatory responses within adipose tissue.

脂肪组织去泛素化酶CYLD失活小鼠的表型分析。
圆筒状瘤病肿瘤抑制因子(CYLD)是一种与脂质代谢有关的去泛素化酶。更具体地说,CYLD与黑腹果蝇的脂质稳态有关,而哺乳动物的CYLD缺乏与肝脏脂质代谢失调有关。综合组织RNA表达分析显示,Cyld mRNA在脂肪组织和肝脏中的表达水平相当,这两个器官与骨骼肌一起主要调节脂质稳态。在本研究中,我们研究了CYLD在哺乳动物脂肪组织稳态和功能中的作用,利用CYLD失活的相关条件小鼠模型,允许组织特异性消除CYLD的催化结构域。与喂食正常或高脂肪食物的对照组相比,突变小鼠的增重率较低。冠状结构(CLS)的组织学分析表明突变体白色脂肪组织的炎症反应减少。我们的数据共同表明,CYLD在调节脂肪组织的关键代谢参数和调节炎症反应中起着关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell and Tissue Research
Cell and Tissue Research 生物-细胞生物学
CiteScore
7.00
自引率
2.80%
发文量
142
审稿时长
1 months
期刊介绍: The journal publishes regular articles and reviews in the areas of molecular, cell, and supracellular biology. In particular, the journal intends to provide a forum for publishing data that analyze the supracellular, integrative actions of gene products and their impact on the formation of tissue structure and function. Submission of papers with an emphasis on structure-function relationships as revealed by recombinant molecular technologies is especially encouraged. Areas of research with a long-standing tradition of publishing in Cell & Tissue Research include: - neurobiology - neuroendocrinology - endocrinology - reproductive biology - skeletal and immune systems - development - stem cells - muscle biology.
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