Cell and Tissue Research最新文献

Sucrose- and fat-related metabolic states influence the adaptation of the pulmonary lipid metabolism to hypoxia.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 DOI: 10.1007/s00441-025-03968-0

Sophia Pankoke, Lea Gerling, Matthias Ochs, Christian Mühlfeld, Julia Schipke

{"title":"Sucrose- and fat-related metabolic states influence the adaptation of the pulmonary lipid metabolism to hypoxia.","authors":"Sophia Pankoke, Lea Gerling, Matthias Ochs, Christian Mühlfeld, Julia Schipke","doi":"10.1007/s00441-025-03968-0","DOIUrl":"https://doi.org/10.1007/s00441-025-03968-0","url":null,"abstract":"Pulmonary surfactant is essential for lung function and consists mainly of lipids, almost half of which in adult mammals originate from de novo synthesis in alveolar epithelial type-2 (AE2) cells. Obesogenic nutrition and hypoxia coexist in obese patients with chronic lung diseases. This study tested the hypothesis that diet-induced obesity and chronic hypoxia alter lipid metabolism and thereby deteriorate surfactant homeostasis. Male C57BL/6N mice were fed control diet (4% fat, 6% sucrose; CD), high-sucrose diet (4% fat, 46% sucrose; HSD) or high-fat diet (35% fat, 7% sucrose; HFD). After 27 weeks, half of each diet group was exposed to hypoxia (13% O2, Hyp) for 3 weeks. After 30 weeks, lung mechanics were assessed, and the blood, livers, and lungs were analyzed. In CD-fed mice, hypoxia induced lung mechanical changes indicative of reduced elastic recoil properties, as well as smaller lamellar bodies (LBs) and higher composite body volumes, suggesting an increased surfactant precursor formation. HSD and HFD induced lipid accumulation in liver and AE2 cells. In HSD-Hyp and HFD-Hyp, LB volumes per alveolar surface area were elevated, indicating compensatory increases in intracellular surfactant pools which were absent in CD-Hyp. Additionally, hypoxia-related lung mechanics alterations were less pronounced in HSD-Hyp and HFD-Hyp. Lung proteome analysis revealed that only a few lipid metabolism-associated proteins were similarly regulated within diet groups under hypoxia, with the most prominent changes in sucrose-fed hypoxic animals. Thus, individual diet-related metabolic states specifically affect the adaptation of the pulmonary lipid metabolism and intracellular surfactant assembly to chronic hypoxia.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity-driven musculotendinous remodeling impairs tissue resilience to mechanical damage.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-31 DOI: 10.1007/s00441-025-03967-1

Cesanelli L, Minderis P, Balnyte I, Ratkevicius A, Degens H, Satkunskiene D

{"title":"Obesity-driven musculotendinous remodeling impairs tissue resilience to mechanical damage.","authors":"Cesanelli L, Minderis P, Balnyte I, Ratkevicius A, Degens H, Satkunskiene D","doi":"10.1007/s00441-025-03967-1","DOIUrl":"https://doi.org/10.1007/s00441-025-03967-1","url":null,"abstract":"Obesity has been associated with lower muscle strength-to-body mass ratio. Here, we evaluated the effects of diet-induced obesity on the mechano-structural properties of isolated muscles and tendons. Thirty 10-week-old male C57BL/6 J mice were randomly assigned to either an obesogenic high-fat diet group (OB) for 24 weeks or a control group (CN) maintained on a standard chow diet. Soleus muscle (SOL) and Achilles tendon (AT) specimens were isolated and subjected either to failure testing, 300 cycles of passive stretch-destretch, or isometric twitch contractions. Morpho-structural and protein expression analyses were conducted to assess collagen and adipose tissue accumulation, concentrations of cross-linking factors, and any alterations in the POSTN-TGFβ1-Akt signaling pathway. OB SOL and AT tissues were more fragile than those from CN (p < 0.05). A piecewise linear regression model revealed a tendency for OB tissues to exhibit steeper mechanical property changes within the first 20 cycles compared to CN, followed by a similar plateau phase in both groups. OB SOL-AT complexes showed a slower twitch-contraction-relaxation pattern than CN (p < 0.05). OB tendons and muscles were larger than those of the CN, with muscles featuring bigger fibers, and higher collagen area fraction (p < 0.05). Elevated TGFβ1 and POSTN concentrations were observed in OB tissues (p < 0.05), alongside increased P-Akt and P-4EBP1 expression (p < 0.05). These findings highlight the detrimental effects of obesity on the structural integrity of muscle and tendon tissues and suggest a significant role of POSTN-TGFβ1-Akt signaling in obesity-associated musculotendinous remodeling.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attenuation of intestinal peristalsis with age is attributed to decreased sensitivity of receptors in the enteric nervous system.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-27 DOI: 10.1007/s00441-025-03969-z

Tsukasa Kobayashi, Yuko Takeba, Masanori Ootaki, Yuki Ohta, Keisuke Kida, Taroh Iiri, Naoki Matsumoto

{"title":"Attenuation of intestinal peristalsis with age is attributed to decreased sensitivity of receptors in the enteric nervous system.","authors":"Tsukasa Kobayashi, Yuko Takeba, Masanori Ootaki, Yuki Ohta, Keisuke Kida, Taroh Iiri, Naoki Matsumoto","doi":"10.1007/s00441-025-03969-z","DOIUrl":"https://doi.org/10.1007/s00441-025-03969-z","url":null,"abstract":"Constipation and other digestive disorders are common in older adults. The autonomic nervous system plays a critical role in regulating digestive motility in the intestinal tract. However, studies on age-related changes in autonomic function and receptor expression in the intestinal tract are limited. In this study, we examined the expression of neurotransmitter receptors in the autonomic nervous system and the effects of acetylcholine and β-agonists on intestinal contraction and relaxation in the jejunum of aged rats. Jejunal sections collected from male and female Wistar/ST rats aged 4, 11, and 18 months were analyzed. Immunohistochemical staining and enzyme-linked immunosorbent assay were used to measure the expression of muscarinic acetylcholine receptors (CHRM2 and CHRM3) and β-adrenergic receptors (β2-ADR and β3-ADR). The effects of acetylcholine, isoproterenol, and mirabegron were assessed in the isolated jejunum for each age group. There was no significant difference in CHRM2 receptor expression among the age groups; however, CHRM3 receptor expression decreased with age. Additionally, the sensitivity to acetylcholine-induced contractile responses decreased with age. Although β2-ADR receptor expression did not differ among the age groups, β3-ADR receptor expression increased with age. Despite this, the relaxation response to isoproterenol and mirabegron decreased with age. Our study revealed an age-related decrease in CHRM3 expression and the contractile response to acetylcholine in the small intestine of rats. Although β-ADR expression, particularly β3-ADR, increased with age, the relaxation response to β-adrenergic agonists gradually decreased.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The large milkweed bugs' Na,K-ATPase β-subunits colocalize with septate junction proteins in a tissue-specific manner.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-26 DOI: 10.1007/s00441-025-03965-3

Marlena Herbertz, Christian Lohr, Susanne Dobler

{"title":"The large milkweed bugs' Na,K-ATPase β-subunits colocalize with septate junction proteins in a tissue-specific manner.","authors":"Marlena Herbertz, Christian Lohr, Susanne Dobler","doi":"10.1007/s00441-025-03965-3","DOIUrl":"https://doi.org/10.1007/s00441-025-03965-3","url":null,"abstract":"The Na,K-ATPase is a vital transmembrane enzyme, which is important for maintaining cell membrane potentials and the general functionality of animal cells. The enzyme's minimal functional unit consists of one α and one β-subunit, whereas the number of existing paralogs varies in different insect species. The functional roles of different β-subunits, which can account for their diversity within a single species, are so far only partially explained. The emphasis of this study was to specifically elucidate the involvement in septate junctions of the four β-subunits of the new model system Oncopeltus fasciatus. Septate junctions function as a paracellular barrier controlling the flow of solutes across epithelia. So far, studies in Drosophila revealed that nervana2, the β2 homolog of Drosophila, is involved in septate junction formation. In O. fasciatus, we demonstrate that most of the Na,K-ATPase subunits colocalize with septate junction proteins. This agrees with our previous findings implying a role of β2 in the control of tracheal tube size in O. fasciatus, which according to the findings in Drosophila appears to be dependent on a stable formation of septate junctions. Finally, our data suggest a connection between the septate junction protein coracle and the enigmatic, N-terminally strongly truncated βx, which has no obvious homologs in other insects. Our study proposes that the four β-subunits form functional units with septate junction proteins, either allowing tissue-adjusted formation of cell-cell contacts or other yet unknown functions.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered morphology of mucosa-associated lymphoid tissues and epithelium in the nasal cavity and lacrimal apparatus in autoimmune disease-prone MRL/MpJ-Fas^lpr/lpr mice.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-25 DOI: 10.1007/s00441-025-03966-2

Masaya Hiraishi, Takashi Namba, Teppei Nakamura, Md Zahir Uddin Rubel, Yasuhiro Kon, Osamu Ichii

{"title":"Altered morphology of mucosa-associated lymphoid tissues and epithelium in the nasal cavity and lacrimal apparatus in autoimmune disease-prone MRL/MpJ-Faslpr/lpr mice.","authors":"Masaya Hiraishi, Takashi Namba, Teppei Nakamura, Md Zahir Uddin Rubel, Yasuhiro Kon, Osamu Ichii","doi":"10.1007/s00441-025-03966-2","DOIUrl":"https://doi.org/10.1007/s00441-025-03966-2","url":null,"abstract":"The mucosal epithelium and the mucosa-associated lymphoid tissues (MALTs) protect the mucosa, such as eye and nose, from constant exposure to foreign antigens. As autoimmune disorders can target both epithelium and MALTs, we morphologically investigated the head of MRL/MpJ-Faslpr/lpr, an autoimmune disease-prone mouse, to discuss the pathological crosstalk among autoimmune disorders, mucosal epithelium, and MALTs. Compared to healthy control MRL/MpJ mice, MRL/MpJ-Faslpr/lpr mice had more lymphoid tissues that were diffusely localized beneath the mucosa epithelium in their lacrimal tracts and nasal cavity. Particularly, lacrimal duct- and nasopharynx-associated lymphoid tissues (LDALT, NALT) were identified as the major MALTs in the mouse head. In the nasal mucosa, MRL/MpJ-Faslpr/lpr mice exhibited larger NALT, more frequent non-ciliated epithelial cells, and more goblet cells than in MRL/MpJ mice. NALT in MRL/MpJ-Faslpr/lpr mice contained more proliferating cells than in MRL/MpJ mice. Moreover, LDALT in some MRL/MpJ-Faslpr/lpr mice developed by being directly connected to the bone marrow surrounding the nasolacrimal duct. These data suggested systemic autoimmune disorders could alter the mucosal immunity of the head by affecting both mucosal epithelium and MALTs. These findings are crucial for understanding the pathology of the head mucosal immunity.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of hydroxyapatite nanoparticles on the cellular processes of stem cells derived from dental tissue sources.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-18 DOI: 10.1007/s00441-025-03962-6

Mais Emad, Mohammad Alnatour, Walhan Alshaer, Jennifer L Gibbs, Benoît Michot, Dana Alqudah, Alaa A A Aljabali, Mairvat Al-Mrahleh, Abdolelah Jaradat, Duaa Abuarqoub

{"title":"Impact of hydroxyapatite nanoparticles on the cellular processes of stem cells derived from dental tissue sources.","authors":"Mais Emad, Mohammad Alnatour, Walhan Alshaer, Jennifer L Gibbs, Benoît Michot, Dana Alqudah, Alaa A A Aljabali, Mairvat Al-Mrahleh, Abdolelah Jaradat, Duaa Abuarqoub","doi":"10.1007/s00441-025-03962-6","DOIUrl":"https://doi.org/10.1007/s00441-025-03962-6","url":null,"abstract":"Hydroxyapatite nanoparticle (HANPs) utilization has recently been notable in bone tissue engineering. This surge owes itself to the biocompatibility of HANPs and their striking resemblance to the minerals found in natural bone. Furthermore, dental pulp-derived stem cells (DPSCs) have garnered attention due to their remarkable differentiation potential into multilineages, thus positioning them as a pivotal cell reservoir for regenerative medicine. This study aims to investigate the impact of HANPs on DPSCs cellular processes. The HANPs have been synthesized using the wet chemical precipitation method followed by freeze-drying and characterization using dynamic light scattering (DLS) and transmission electron microscopy (TEM). The size of HANPs was reported to be in the range of 55-67 nm. Our dataset divulges that DPSCs can endure concentrations of HANPs up to ≤ 0.81 mg/mL without incurring any conspicuous alterations in their morphology or the pace of proliferation. Furthermore, the self-renewal potency of HANPs was upheld at concentrations ≤ 0.20 mg/mL. Flow cytometric analysis affirms a significant divergence in cell distribution across all cell cycle phases in DPSCs treated with 0.81 mg/mL HANPs. Intriguingly, no variance surfaced in the migratory capacity of DPSCs exposed to HANPs of ≤ 0.40 mg/mL. For osteogenic differentiation, HANPs at concentrations of ≤ 0.40 mg/mL demonstrated the aptitude to incite osteogenic differentiation within DPSCs, facilitating the formation of calcium deposits. In conclusion, combining HANPs and DPSCs shows promise for restoring damaged hard tissues, like bone and teeth, and enhancing regenerative therapies.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Microtubule organization and tubulin post‑translational modifications in intact tissues and during regeneration in calcareous sponges.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-14 DOI: 10.1007/s00441-025-03964-4

Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov

引用次数: 0

Long-term consumption of moderate amounts of sucrose-sweetened drinks disrupts intestinal barrier function by impairing goblet cell differentiation.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-12 DOI: 10.1007/s00441-025-03961-7

Sachiko Sato, Arif U Hasan, Mami Obara, Yukiko Kondo, Eiichi Taira

{"title":"Long-term consumption of moderate amounts of sucrose-sweetened drinks disrupts intestinal barrier function by impairing goblet cell differentiation.","authors":"Sachiko Sato, Arif U Hasan, Mami Obara, Yukiko Kondo, Eiichi Taira","doi":"10.1007/s00441-025-03961-7","DOIUrl":"https://doi.org/10.1007/s00441-025-03961-7","url":null,"abstract":"While the prolonged consumption of sucrose-containing beverages is known to impact many organs, their specific effects on the small intestine remain elusive. This study aimed to evaluate how regular intake of sucrose, in amounts typically consumed, affects goblet cells, which play a critical role in regulating the mucosal barrier and innate immune defenses in the small intestine. Ten-week-old male ddY mice, a model of diet-induced obesity, were given a regular diet with either plain water or 7% sucrose water. Caloric intake was monitored weekly through food and drink measurements. After 8 weeks, glucose and insulin responses were evaluated following an oral gavage of glucose or sucrose. At 14 weeks, plasma, whole small intestine, and liver samples were collected. Despite achieving an isocaloric state, mice drinking sucrose water showed approximately a 1.5-fold increase in body weight and impaired glucose tolerance. In the small intestine, genes involved in sucrose digestion and absorption (Sis, Sglt1, Glut2, and Glut5) were upregulated, while genes essential for maintaining the intestinal barrier and function (Epcam, Fabp2, Cldn1, Ocln, and Tjp1) were downregulated. Serum levels and mRNA expression of the inflammatory cytokine, interleukin-18 were elevated. Genes responsible for goblet cell differentiation and function (Hes1, Gfi1, Spdef, and Klf4) were downregulated, leading to an increase in immature goblet cells and a decrease in mucin-producing markers (Muc2, Muc4, and Muc13) in the jejunum. The findings underscore that besides obesity, long-term intake of sucrose-containing drinks provokes localized inflammation and disrupts small intestinal barrier function by impairing goblet cell differentiation and activity.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-10 DOI: 10.1007/s00441-025-03955-5

Kaaj Pala, Kevin Xueying Sun, Lars Krogvold, Knut Dahl-Jørgensen, Shiva Reddy

{"title":"Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease.","authors":"Kaaj Pala, Kevin Xueying Sun, Lars Krogvold, Knut Dahl-Jørgensen, Shiva Reddy","doi":"10.1007/s00441-025-03955-5","DOIUrl":"https://doi.org/10.1007/s00441-025-03955-5","url":null,"abstract":"During type 1 diabetes (T1D), oxidative stress in beta cells may cause early beta cell dysfunction and initiate autoimmunity. Mouse islets express lower levels of reactive oxygen species (ROS) clearing enzymes, such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase than several other tissues. It remains unclear if human beta cells show a similar deficiency during T1D or exhibit a higher degree of intrinsic resistance to oxidative stress. We compared islet cell distributions and determined graded intensities of glutathione peroxidase1 (GPX1), a key enzymatic mediator involved in detoxifying hydrogen peroxide, by applying combined immunohistochemistry for GPX1, insulin and glucagon, in pancreatic sections from new-onset T1D (group 1), non-diabetic autoantibody-negative (group 2), non-diabetic autoantibody-positive (group 3) and long-term diabetic (group 4) donors. Islets from all study groups demonstrated either uniform but graded staining intensities for GPX1 in almost all islet cells or strong staining in selective islet cells with weaker intensities in the remaining cells. GPX1 was present in selective glucagon cells and insulin cells, including in cells negative for both hormones, with stronger intensities in a higher percentage of glucagon than insulin cells. It was absent in a higher percentage of beta cells than glucagon cells independent of disease or autoantibody positivity. We conclude that a proportion of human beta cells and glucagon cells express GPX1 but show heterogeneity in its distribution and intensities, independent of disease or autoantibody status. Our studies highlight important differences in the expression of GPX1 in islet cell-types between mice and humans.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-05 DOI: 10.1007/s00441-025-03960-8

Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov

{"title":"Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.","authors":"Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov","doi":"10.1007/s00441-025-03960-8","DOIUrl":"10.1007/s00441-025-03960-8","url":null,"abstract":"Microtubules are the principal cytoskeletal component in cells, integral to various morphogenetic processes in Metazoa, including cell migration, adhesion, and polarity. Their dynamics and functions are modulated by tubulin post-translational modifications (PTMs). While studies on model species have provided insights into microtubule functions, understanding their evolutionary aspects necessitates exploring non-model organisms. Sponges (phylum Porifera) are an early-branching metazoan group with outstanding regenerative capacities. This research presents the first comprehensive analysis of microtubule organization and tubulin PTMs in calcareous sponges. The intact sponge cells show various but typical types of microtubule organization, while detected tubulin PTMs are associated with certain cell types, indicating specific functions in particular cellular contexts. During regeneration, relying on the coordinated movement of epithelial-like cell sheets, microtubule networks in exopinacocytes and choanocytes undergo significant reorganization. These rearranged microtubules potentially stabilize cellular migration direction and facilitate cargo transport, essential for cell contact and polarity establishment. This study enhances our understanding of microtubule functionality and regulation in early-diverging metazoans, contributing to the broader evolutionary context of cytoskeletal dynamics.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0