Cell and Tissue Research最新文献

Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue. 脂肪组织去泛素化酶CYLD失活小鼠的表型分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-02 DOI: 10.1007/s00441-025-03989-9

Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos

{"title":"Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue.","authors":"Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos","doi":"10.1007/s00441-025-03989-9","DOIUrl":"https://doi.org/10.1007/s00441-025-03989-9","url":null,"abstract":"The cylindromatosis tumor suppressor (CYLD) is a deubiquitinating enzyme that has been implicated in lipid metabolism. More specifically, CYLD has been associated with lipid homeostasis in Drosophila melanogaster, and CYLD deficiency in mammals has been linked to dysregulation of lipid metabolism in the liver. Comprehensive tissue RNA expression analyses have revealed comparable levels of Cyld mRNA expression in the adipose tissue and liver, the organs that, together with skeletal muscle, primarily regulate lipid homeostasis. In the present study, the role of CYLD in mammalian adipose tissue homeostasis and function was investigated, utilizing a relevant conditional mouse model of CYLD inactivation that permits tissue-specific elimination of the catalytic domain of CYLD. Mutant mice displayed reduced weight-gain rate compared to controls when fed a normal or high-fat diet. Histological analysis of crown-like structures (CLS) indicated a reduced inflammatory response in the white adipose tissue of mutants. Our data collectively demonstrate that CYLD plays a pivotal role in regulating key metabolic parameters and modulating inflammatory responses within adipose tissue.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid-liquid phase separation in normal hematopoiesis and hematological diseases. 液-液相分离在正常造血和血液系统疾病中的应用。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-05-17 DOI: 10.1007/s00441-025-03974-2

Lexuan Lin, Fang He, Xu Jin, Xiaoru Zhang, Yue Li, Di Wang, Jingwei Wang, Lingyue Zheng, Haoze Song, Xu Zhu, Qimei Cheng, Yitong Zhao, Jing Liang, Jinfa Ma, Jie Gao, Jingyuan Tong, Lihong Shi

{"title":"Liquid-liquid phase separation in normal hematopoiesis and hematological diseases.","authors":"Lexuan Lin, Fang He, Xu Jin, Xiaoru Zhang, Yue Li, Di Wang, Jingwei Wang, Lingyue Zheng, Haoze Song, Xu Zhu, Qimei Cheng, Yitong Zhao, Jing Liang, Jinfa Ma, Jie Gao, Jingyuan Tong, Lihong Shi","doi":"10.1007/s00441-025-03974-2","DOIUrl":"10.1007/s00441-025-03974-2","url":null,"abstract":"Liquid-liquid phase separation (LLPS) is an emerging research field in cellular biology. LLPS-driven biomolecular condensates act as reaction chambers and regulatory hubs for critical processes, including chromatin architecture, gene expression, and metabolism. The dysregulation of these processes frequently impedes the proper execution of physiological functions. Current research indicates that abnormal phase separation plays a significant role in the pathogenesis of diseases and aging. This review briefly overviews the fundamental concepts and research methods related to phase separation. We also summarize studies concerning its physiological functions, particularly emphasizing its role in hematopoiesis. We further discuss how abnormal phase separation can lead to hematological disorders, specifically summarizing its involvement in the pathogenesis of leukemia. Despite recent advancements, elucidating LLPS mechanisms in hematopoiesis remains challenging due to the intricate interplay between biomolecular condensates and cellular function. Future research efforts aiming to reveal the role of LLPS in hematological diseases hold promise for novel therapeutic interventions and a deeper understanding of hematopoietic processes.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"15-27"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection. COVID-19急性感染患者呼吸肌组织病理学分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-05-05 DOI: 10.1007/s00441-025-03973-3

Laura Steingruber, Simona Handtke, Franziska Schweiger, Stefan Schiele, Bruno Märkl, Marco Koch

{"title":"Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection.","authors":"Laura Steingruber, Simona Handtke, Franziska Schweiger, Stefan Schiele, Bruno Märkl, Marco Koch","doi":"10.1007/s00441-025-03973-3","DOIUrl":"10.1007/s00441-025-03973-3","url":null,"abstract":"Coronavirus-disease 2019 (COVID-19) affects the respiratory system with high morbidity in elderly and comorbid patients. Acute COVID-19 infection (CI) primarily leads to respiratory failure, long-term effects on respiratory skeletal muscle however remain vague. Thus, histopathological marker expression of oxidative stress, inflammation, satellite cell activity, myosin fiber composition, and cellular senescence were analyzed in intercostal muscle and diaphragm to compare respiratory muscle degeneration (RMD) in deceased CI-positive and control patients. Beside CI, the impact of BMI, age, sex, ventilation status, and duration of hospitalization on RMD were evaluated. CI-positive patients exhibited higher numbers of regenerative stem cells, but no association between CI status and RMD was observed. Interestingly, ventilation support and lung-associated comorbidities had no effect on expression of RMD markers (p > 0.05). However, intercostal muscle showed BMI-dependent changes in expression of RMD markers, regardless of the CI status, with increased cytokine expression (p = 0.04), reduced antioxidative capacity (p = 0.05), and low stem cell prevalence (p = 0.02) in patients with high BMI. Moreover, elderly patients demonstrated increased oxidative stress (p = 0.001) and cell senescence (p = 0.03) independent of CI status. Notably, immobility drives muscle fiber transformation to Myosin ST (p = 0.03), since prolonged hospitalization correlated with muscle fiber type shift. Limitations included incomplete retrospective data collection and absence of adequate samples for molecular analyses. Together, RMD is influenced by BMI, age and immobility rather than the CI status alone. Future studies including larger cohorts, molecular analyses, and evaluation of patient data in addition to CI status alone, will further support meaningful analyses and interpretation of RMD and its impact on post CI recovery.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"59-68"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain morphology in the peracarid crustacean Neomysis integer (Leach, 1814) with an emphasis on sexual dimorphism of the olfactory pathway. 过硬壳类新解虫的脑形态学（Leach, 1814），重点研究嗅觉通路的两性二态性。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-05-14 DOI: 10.1007/s00441-025-03978-y

Katja Kümmerlen, Johanna Blatt, Lena Hoffmann, Steffen Harzsch

{"title":"Brain morphology in the peracarid crustacean Neomysis integer (Leach, 1814) with an emphasis on sexual dimorphism of the olfactory pathway.","authors":"Katja Kümmerlen, Johanna Blatt, Lena Hoffmann, Steffen Harzsch","doi":"10.1007/s00441-025-03978-y","DOIUrl":"10.1007/s00441-025-03978-y","url":null,"abstract":"Our current understanding of brain organization in malacostracan crustaceans is strongly biased towards representatives of the Decapoda (\"ten legged\" crustaceans) such as crayfish, crabs, clawed lobsters and spiny lobsters. However, to understand aspects of brain evolution in crustaceans, a broader taxonomic sampling is essential. The peracarid crustaceans are a species-rich group that embraces representatives of, e.g. the Isopoda, Amphipoda and Mysida (\"opossum shrimps\"), taxa whose neuroanatomy has not been carefully examined. The current study sets out to analyze brain morphology of the mysid Neomysis integer (Leach, 1814; Peracarida, Mysida) using immunohistochemistry against the presynaptic protein synapsin and the neuropeptides RFamide, SIFamide and allatostatin combined with three-dimensional reconstruction of elements of the central olfactory pathway. Furthermore, we studied the inventory of sensilla on the first pair of antennae using cuticular autofluorescence. Anterograde filling with neuronal tracers allowed visualisation the central projections of the sensilla on the first pair of antennae. This species is known to display a sexual dimorphism in both the peripheral and central olfactory pathway. We focussed our analysis on this aspect because in contrast to Hexapoda, reports on a sexual dimorphism of the olfactory system are extremely rare in malacostracan crustaceans. We provide a detailed description of the sensilla associated with a male-specific structure on the pair of first antenna the \"lobus masculinus\". Furthermore, we analyzed the projection patterns of theses sensilla into the \"male-specific neuropil\" in the deutocerebrum and critically discuss our results in comparison to examples of sexual dimorphism in the chemosensory pathways in other malacostracan crustaceans and hexapods.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"95-115"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of chorionic mesenchymal stromal cells, their conditioned medium, and membrane particles on neutrophil functionality. 绒毛膜间充质间质细胞及其条件介质和膜颗粒对中性粒细胞功能的影响。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-04-22 DOI: 10.1007/s00441-025-03970-6

Monique Maria Franco-da-Silva, Mariana Rauback Aubin, Alessandra Amaral de Vasconcellos, Dienifer Hermann Sirena, Giovana Bangel Marchaki, Laíza Rief Ruggeri, André Ferreira Hennigen, Thaís Muradás, Ana Beatriz Tittoni da Silveira, Elizandra Braganhol, Roselena Silvestri Schuh, Guilherme Baldo, Anelise Bergmann Araújo, Ana Helena Paz

{"title":"Effects of chorionic mesenchymal stromal cells, their conditioned medium, and membrane particles on neutrophil functionality.","authors":"Monique Maria Franco-da-Silva, Mariana Rauback Aubin, Alessandra Amaral de Vasconcellos, Dienifer Hermann Sirena, Giovana Bangel Marchaki, Laíza Rief Ruggeri, André Ferreira Hennigen, Thaís Muradás, Ana Beatriz Tittoni da Silveira, Elizandra Braganhol, Roselena Silvestri Schuh, Guilherme Baldo, Anelise Bergmann Araújo, Ana Helena Paz","doi":"10.1007/s00441-025-03970-6","DOIUrl":"10.1007/s00441-025-03970-6","url":null,"abstract":"Mesenchymal stromal cells (MSC) are multipotent cells that can modulate immune cells, affecting macrophages, monocytes, and lymphocytes. Neutrophils are circulating leucocytes responsible for the first line of defense and can assume different phenotypes depending on their environment: N0, the naïve form, N1 (inflammatory), N2 (anti-inflammatory). This study explores the potentially protective roles of chorionic membrane MSCs and their products-conditioned medium and pre-conditioned cMSC-derived membrane microparticles (MP-cMSC)-on neutrophils. Conditioned medium treatment reduced the rate of apoptosis and enhanced the immunosuppressive potential consistent with an anti-inflammatory profile. MP-cMSC are a noteworthy cell-free therapy, consisting of artificially generated circular lipid bilayer structures with no cargo and approximately 200 nm in size. When added to neutrophil culture, MPs increased neutral red uptake, suggesting an enhanced phagocytic activity. In the MSC co-culture group, a reduced rate of apoptosis, increased neutral red uptake, and elevated programed death-ligand 1 (PD-L1) expression were observed. These findings suggest that the distinct effects elicited by conditioned media, microparticles, and co-culture are likely influenced by the specific nature of the interactions involved-whether purely paracrine, mediated through direct cell-to-cell contact, or a combination of both.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"43-57"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ETS1 promotes the expression of Ctsb and Mmp13 during the differentiation of septoclasts from pericytes. 在中隔细胞向周细胞分化过程中，ETS1促进Ctsb和Mmp13的表达。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-05-19 DOI: 10.1007/s00441-025-03979-x

Yasuhiko Bando, Kenjiro Bandow, Koji Sakiyama, Arata Nagasaka, Kaito Suzuki, Miyuki Toda-Fujii, Yuji Owada, Osamu Amano

{"title":"ETS1 promotes the expression of Ctsb and Mmp13 during the differentiation of septoclasts from pericytes.","authors":"Yasuhiko Bando, Kenjiro Bandow, Koji Sakiyama, Arata Nagasaka, Kaito Suzuki, Miyuki Toda-Fujii, Yuji Owada, Osamu Amano","doi":"10.1007/s00441-025-03979-x","DOIUrl":"10.1007/s00441-025-03979-x","url":null,"abstract":"Septoclasts (SCs), which express both fatty acid-binding protein 5 and platelet-derived growth factor beta, are mononuclear cartilage-resorbing cells predominantly located at the chondro-osseous junction of the growth plate (GP). These cells originate from pericytes (PCs). Cathepsin B (CTSB) and matrix metalloproteinase-13 (MMP13), expressed in SCs, participate in the degradation of collagen and other cartilage matrices. This study aimed to investigate the involvement of the ETS proto-oncogene 1 (ETS1) in the transcription of Ctsb and Mmp13 during the differentiation of SCs from PCs. ETS1 was localized in SCs and a small number of PCs during development and postnatal stages. Upregulation of Ets1, Mmp13, Ctsb, and the Ets1-related genes, specificity protein 1 (Sp-1), jun proto-oncogene (c-Jun), and cAMP response element-binding protein-binding protein (Crebbp) in SCs compared with those in PCs was shown by RNA-seq analysis of samples isolated from the tibiae of 3-week-old postnatal mice. The Ets1-related proteins were localized ubiquitously in SCs and PCs in the GP. In primary SC cultures, the expression levels of Ctsb and Mmp13 were significantly reduced following treatment with Ets1 siRNA. Thus, our results revealed that ETS1 promoted the expression of Ctsb and Mmp13 in SCs during the differentiation of SCs from PCs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"29-42"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sucrose- and fat-related metabolic states influence the adaptation of the pulmonary lipid metabolism to hypoxia. 蔗糖和脂肪相关的代谢状态影响肺脂质代谢对缺氧的适应。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-04-01 DOI: 10.1007/s00441-025-03968-0

Sophia Pankoke, Lea Gerling, Matthias Ochs, Christian Mühlfeld, Julia Schipke

{"title":"Sucrose- and fat-related metabolic states influence the adaptation of the pulmonary lipid metabolism to hypoxia.","authors":"Sophia Pankoke, Lea Gerling, Matthias Ochs, Christian Mühlfeld, Julia Schipke","doi":"10.1007/s00441-025-03968-0","DOIUrl":"10.1007/s00441-025-03968-0","url":null,"abstract":"Pulmonary surfactant is essential for lung function and consists mainly of lipids, almost half of which in adult mammals originate from de novo synthesis in alveolar epithelial type-2 (AE2) cells. Obesogenic nutrition and hypoxia coexist in obese patients with chronic lung diseases. This study tested the hypothesis that diet-induced obesity and chronic hypoxia alter lipid metabolism and thereby deteriorate surfactant homeostasis. Male C57BL/6N mice were fed control diet (4% fat, 6% sucrose; CD), high-sucrose diet (4% fat, 46% sucrose; HSD) or high-fat diet (35% fat, 7% sucrose; HFD). After 27 weeks, half of each diet group was exposed to hypoxia (13% O2, Hyp) for 3 weeks. After 30 weeks, lung mechanics were assessed, and the blood, livers, and lungs were analyzed. In CD-fed mice, hypoxia induced lung mechanical changes indicative of reduced elastic recoil properties, as well as smaller lamellar bodies (LBs) and higher composite body volumes, suggesting an increased surfactant precursor formation. HSD and HFD induced lipid accumulation in liver and AE2 cells. In HSD-Hyp and HFD-Hyp, LB volumes per alveolar surface area were elevated, indicating compensatory increases in intracellular surfactant pools which were absent in CD-Hyp. Additionally, hypoxia-related lung mechanics alterations were less pronounced in HSD-Hyp and HFD-Hyp. Lung proteome analysis revealed that only a few lipid metabolism-associated proteins were similarly regulated within diet groups under hypoxia, with the most prominent changes in sucrose-fed hypoxic animals. Thus, individual diet-related metabolic states specifically affect the adaptation of the pulmonary lipid metabolism and intracellular surfactant assembly to chronic hypoxia.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"69-81"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Olfactory receptors and human diseases. 嗅觉受体和人类疾病。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-04-25 DOI: 10.1007/s00441-025-03971-5

Zhong-Qi Yuan, Xiao-Chun Peng, Lian Liu, Fu-Yuan Yang, Feng Qian

{"title":"Olfactory receptors and human diseases.","authors":"Zhong-Qi Yuan, Xiao-Chun Peng, Lian Liu, Fu-Yuan Yang, Feng Qian","doi":"10.1007/s00441-025-03971-5","DOIUrl":"10.1007/s00441-025-03971-5","url":null,"abstract":"Olfaction plays a crucial role in distinguishing odors, enabling organisms to seek benefits and evade hazards. Olfactory receptors (ORs), characterized by highly variable binding pockets, facilitate the detection of diverse odorants from both external and internal environments. Nasal ORs, expressed in olfactory sensory neurons (OSNs), are critical for olfactory cognition and associated neuronal plasticity. In contrast, extra-nasal ORs, expressed in extra-olfactory tissues, detect specific chemicals and modulate cellular processes such as proliferation, migration, inflammation, and apoptosis. Aberrant OR expression or dysfunction has been implicated in numerous human diseases, including anosmia, dementia, dermatopathies, obesity, infertility, cancers, respiratory disorders, atherosclerosis and viral infections. Olfactory training, such as aromatherapy, demonstrates significant therapeutic potential for anosmia, dementia and psychological distress. Natural or synthetic odorants have been applied for promoting hair regeneration and cutaneous wound healing. Conversely, overexpression of specific ORs in cancer cells may drive tumor progression. Additionally, ORs may mediate virus-host interactions during infection, owing to their structural variability. Collectively, OR-targeted agonists and antagonists (odorants) represent promising candidates for treating OR-associated pathologies.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"1-14"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRAMEY enhances sperm-egg binding and modulates epigenetic dynamics in bovine embryogenesis. PRAMEY增强精子-卵子结合和调节牛胚胎发生的表观遗传动力学。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-05-14 DOI: 10.1007/s00441-025-03975-1

Chandlar Kern, Wan-Sheng Liu

{"title":"PRAMEY enhances sperm-egg binding and modulates epigenetic dynamics in bovine embryogenesis.","authors":"Chandlar Kern, Wan-Sheng Liu","doi":"10.1007/s00441-025-03975-1","DOIUrl":"10.1007/s00441-025-03975-1","url":null,"abstract":"Infertility and subfertility are significant reproductive challenges in cattle, often linked to genetic factors. Among these genetic factors, the bovine Y-linked gene family, PRAMEY, has emerged as a candidate due to its involvement in germ cell formation, fertilization, and embryonic development. This study investigates PRAMEY's role in sperm-egg binding, acrosome integrity, and epigenetic modifications during fertilization and early embryogenesis. Using IVF with bovine spermatozoa treated with either PRAMEY antibody (ab) or rabbit IgG control, we assessed sperm-egg binding and acrosome integrity at 2, 4, and 6 h post-fertilization (hpf). PRAMEY ab treatment doubled sperm binding per oocyte across all time points, with a significant increase at 6 hpf (P ≤ 0.05), although no differences in acrosome integrity were observed (P > 0.05). To explore PRAMEY's role in epigenetic regulation, we analyzed DNA (5-methylcytosine (5-mC)) and histone (H3K9me3 and H3K27me3) methylation in zygotes and embryos using immunofluorescent staining techniques. Zygotes derived from PRAMEY ab-treated spermatozoa showed significantly reduced DNA methylation in paternal pronuclei at 10 hpf and maternal pronuclei at 25 hpf (P ≤ 0.01). Histone methylation analysis revealed no significant differences in H3K9me3 methylation between groups, but H3K27me3 methylation was significantly lower in embryos produced using PRAMEY ab-treated spermatozoa at the 8-cell and morula stages (P ≤ 0.05). In summary, PRAMEY inhibition enhances sperm-egg binding and influences DNA and histone methylation dynamics in bovine embryos, underscoring its potential role in fertilization and early embryonic epigenetic regulation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"83-94"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enteroepithelial stages of Toxoplasma gondii in the ileum of cats by serial block face scanning electron microscopy. 用连续块面扫描电镜观察猫回肠刚地弓形虫肠上皮分期。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 DOI: 10.1007/s00441-025-03988-w

Marcia Attias, Wanderley de Souza, Jitender P Dubey

引用次数: 0