Cell and Tissue Research最新文献

Liquid-liquid phase separation in normal hematopoiesis and hematological diseases. 液-液相分离在正常造血和血液系统疾病中的应用。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-17 DOI: 10.1007/s00441-025-03974-2

Lexuan Lin, Fang He, Xu Jin, Xiaoru Zhang, Yue Li, Di Wang, Jingwei Wang, Lingyue Zheng, Haoze Song, Xu Zhu, Qimei Cheng, Yitong Zhao, Jing Liang, Jinfa Ma, Jie Gao, Jingyuan Tong, Lihong Shi

{"title":"Liquid-liquid phase separation in normal hematopoiesis and hematological diseases.","authors":"Lexuan Lin, Fang He, Xu Jin, Xiaoru Zhang, Yue Li, Di Wang, Jingwei Wang, Lingyue Zheng, Haoze Song, Xu Zhu, Qimei Cheng, Yitong Zhao, Jing Liang, Jinfa Ma, Jie Gao, Jingyuan Tong, Lihong Shi","doi":"10.1007/s00441-025-03974-2","DOIUrl":"https://doi.org/10.1007/s00441-025-03974-2","url":null,"abstract":"Liquid-liquid phase separation (LLPS) is an emerging research field in cellular biology. LLPS-driven biomolecular condensates act as reaction chambers and regulatory hubs for critical processes, including chromatin architecture, gene expression, and metabolism. The dysregulation of these processes frequently impedes the proper execution of physiological functions. Current research indicates that abnormal phase separation plays a significant role in the pathogenesis of diseases and aging. This review briefly overviews the fundamental concepts and research methods related to phase separation. We also summarize studies concerning its physiological functions, particularly emphasizing its role in hematopoiesis. We further discuss how abnormal phase separation can lead to hematological disorders, specifically summarizing its involvement in the pathogenesis of leukemia. Despite recent advancements, elucidating LLPS mechanisms in hematopoiesis remains challenging due to the intricate interplay between biomolecular condensates and cellular function. Future research efforts aiming to reveal the role of LLPS in hematological diseases hold promise for novel therapeutic interventions and a deeper understanding of hematopoietic processes.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain morphology in the peracarid crustacean Neomysis integer (Leach, 1814) with an emphasis on sexual dimorphism of the olfactory pathway. 过硬壳类新解虫的脑形态学（Leach, 1814），重点研究嗅觉通路的两性二态性。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-14 DOI: 10.1007/s00441-025-03978-y

Katja Kümmerlen, Johanna Blatt, Lena Hoffmann, Steffen Harzsch

{"title":"Brain morphology in the peracarid crustacean Neomysis integer (Leach, 1814) with an emphasis on sexual dimorphism of the olfactory pathway.","authors":"Katja Kümmerlen, Johanna Blatt, Lena Hoffmann, Steffen Harzsch","doi":"10.1007/s00441-025-03978-y","DOIUrl":"https://doi.org/10.1007/s00441-025-03978-y","url":null,"abstract":"Our current understanding of brain organization in malacostracan crustaceans is strongly biased towards representatives of the Decapoda (\"ten legged\" crustaceans) such as crayfish, crabs, clawed lobsters and spiny lobsters. However, to understand aspects of brain evolution in crustaceans, a broader taxonomic sampling is essential. The peracarid crustaceans are a species-rich group that embraces representatives of, e.g. the Isopoda, Amphipoda and Mysida (\"opossum shrimps\"), taxa whose neuroanatomy has not been carefully examined. The current study sets out to analyze brain morphology of the mysid Neomysis integer (Leach, 1814; Peracarida, Mysida) using immunohistochemistry against the presynaptic protein synapsin and the neuropeptides RFamide, SIFamide and allatostatin combined with three-dimensional reconstruction of elements of the central olfactory pathway. Furthermore, we studied the inventory of sensilla on the first pair of antennae using cuticular autofluorescence. Anterograde filling with neuronal tracers allowed visualisation the central projections of the sensilla on the first pair of antennae. This species is known to display a sexual dimorphism in both the peripheral and central olfactory pathway. We focussed our analysis on this aspect because in contrast to Hexapoda, reports on a sexual dimorphism of the olfactory system are extremely rare in malacostracan crustaceans. We provide a detailed description of the sensilla associated with a male-specific structure on the pair of first antenna the \"lobus masculinus\". Furthermore, we analyzed the projection patterns of theses sensilla into the \"male-specific neuropil\" in the deutocerebrum and critically discuss our results in comparison to examples of sexual dimorphism in the chemosensory pathways in other malacostracan crustaceans and hexapods.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRAMEY enhances sperm-egg binding and modulates epigenetic dynamics in bovine embryogenesis. PRAMEY增强精子-卵子结合和调节牛胚胎发生的表观遗传动力学。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-14 DOI: 10.1007/s00441-025-03975-1

Chandlar Kern, Wan-Sheng Liu

{"title":"PRAMEY enhances sperm-egg binding and modulates epigenetic dynamics in bovine embryogenesis.","authors":"Chandlar Kern, Wan-Sheng Liu","doi":"10.1007/s00441-025-03975-1","DOIUrl":"https://doi.org/10.1007/s00441-025-03975-1","url":null,"abstract":"Infertility and subfertility are significant reproductive challenges in cattle, often linked to genetic factors. Among these genetic factors, the bovine Y-linked gene family, PRAMEY, has emerged as a candidate due to its involvement in germ cell formation, fertilization, and embryonic development. This study investigates PRAMEY's role in sperm-egg binding, acrosome integrity, and epigenetic modifications during fertilization and early embryogenesis. Using IVF with bovine spermatozoa treated with either PRAMEY antibody (ab) or rabbit IgG control, we assessed sperm-egg binding and acrosome integrity at 2, 4, and 6 h post-fertilization (hpf). PRAMEY ab treatment doubled sperm binding per oocyte across all time points, with a significant increase at 6 hpf (P ≤ 0.05), although no differences in acrosome integrity were observed (P > 0.05). To explore PRAMEY's role in epigenetic regulation, we analyzed DNA (5-methylcytosine (5-mC)) and histone (H3K9me3 and H3K27me3) methylation in zygotes and embryos using immunofluorescent staining techniques. Zygotes derived from PRAMEY ab-treated spermatozoa showed significantly reduced DNA methylation in paternal pronuclei at 10 hpf and maternal pronuclei at 25 hpf (P ≤ 0.01). Histone methylation analysis revealed no significant differences in H3K9me3 methylation between groups, but H3K27me3 methylation was significantly lower in embryos produced using PRAMEY ab-treated spermatozoa at the 8-cell and morula stages (P ≤ 0.05). In summary, PRAMEY inhibition enhances sperm-egg binding and influences DNA and histone methylation dynamics in bovine embryos, underscoring its potential role in fertilization and early embryonic epigenetic regulation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection. COVID-19急性感染患者呼吸肌组织病理学分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-05 DOI: 10.1007/s00441-025-03973-3

Laura Steingruber, Simona Handtke, Franziska Schweiger, Stefan Schiele, Bruno Märkl, Marco Koch

{"title":"Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection.","authors":"Laura Steingruber, Simona Handtke, Franziska Schweiger, Stefan Schiele, Bruno Märkl, Marco Koch","doi":"10.1007/s00441-025-03973-3","DOIUrl":"https://doi.org/10.1007/s00441-025-03973-3","url":null,"abstract":"Coronavirus-disease 2019 (COVID-19) affects the respiratory system with high morbidity in elderly and comorbid patients. Acute COVID-19 infection (CI) primarily leads to respiratory failure, long-term effects on respiratory skeletal muscle however remain vague. Thus, histopathological marker expression of oxidative stress, inflammation, satellite cell activity, myosin fiber composition, and cellular senescence were analyzed in intercostal muscle and diaphragm to compare respiratory muscle degeneration (RMD) in deceased CI-positive and control patients. Beside CI, the impact of BMI, age, sex, ventilation status, and duration of hospitalization on RMD were evaluated. CI-positive patients exhibited higher numbers of regenerative stem cells, but no association between CI status and RMD was observed. Interestingly, ventilation support and lung-associated comorbidities had no effect on expression of RMD markers (p > 0.05). However, intercostal muscle showed BMI-dependent changes in expression of RMD markers, regardless of the CI status, with increased cytokine expression (p = 0.04), reduced antioxidative capacity (p = 0.05), and low stem cell prevalence (p = 0.02) in patients with high BMI. Moreover, elderly patients demonstrated increased oxidative stress (p = 0.001) and cell senescence (p = 0.03) independent of CI status. Notably, immobility drives muscle fiber transformation to Myosin ST (p = 0.03), since prolonged hospitalization correlated with muscle fiber type shift. Limitations included incomplete retrospective data collection and absence of adequate samples for molecular analyses. Together, RMD is influenced by BMI, age and immobility rather than the CI status alone. Future studies including larger cohorts, molecular analyses, and evaluation of patient data in addition to CI status alone, will further support meaningful analyses and interpretation of RMD and its impact on post CI recovery.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special issue 100th anniversary Cell and Tissue Research. 《细胞与组织研究》100周年特刊。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-01 DOI: 10.1007/s00441-025-03972-4

Horst-Werner Korf, David P Kelsell, Vera Kozjak-Pavlovic

引用次数: 0

Olfactory receptors and human diseases. 嗅觉受体和人类疾病。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-25 DOI: 10.1007/s00441-025-03971-5

Zhong-Qi Yuan, Xiao-Chun Peng, Lian Liu, Fu-Yuan Yang, Feng Qian

{"title":"Olfactory receptors and human diseases.","authors":"Zhong-Qi Yuan, Xiao-Chun Peng, Lian Liu, Fu-Yuan Yang, Feng Qian","doi":"10.1007/s00441-025-03971-5","DOIUrl":"https://doi.org/10.1007/s00441-025-03971-5","url":null,"abstract":"Olfaction plays a crucial role in distinguishing odors, enabling organisms to seek benefits and evade hazards. Olfactory receptors (ORs), characterized by highly variable binding pockets, facilitate the detection of diverse odorants from both external and internal environments. Nasal ORs, expressed in olfactory sensory neurons (OSNs), are critical for olfactory cognition and associated neuronal plasticity. In contrast, extra-nasal ORs, expressed in extra-olfactory tissues, detect specific chemicals and modulate cellular processes such as proliferation, migration, inflammation, and apoptosis. Aberrant OR expression or dysfunction has been implicated in numerous human diseases, including anosmia, dementia, dermatopathies, obesity, infertility, cancers, respiratory disorders, atherosclerosis and viral infections. Olfactory training, such as aromatherapy, demonstrates significant therapeutic potential for anosmia, dementia and psychological distress. Natural or synthetic odorants have been applied for promoting hair regeneration and cutaneous wound healing. Conversely, overexpression of specific ORs in cancer cells may drive tumor progression. Additionally, ORs may mediate virus-host interactions during infection, owing to their structural variability. Collectively, OR-targeted agonists and antagonists (odorants) represent promising candidates for treating OR-associated pathologies.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of chorionic mesenchymal stromal cells, their conditioned medium, and membrane particles on neutrophil functionality. 绒毛膜间充质间质细胞及其条件介质和膜颗粒对中性粒细胞功能的影响。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-22 DOI: 10.1007/s00441-025-03970-6

Monique Maria Franco-da-Silva, Mariana Rauback Aubin, Alessandra Amaral de Vasconcellos, Dienifer Hermann Sirena, Giovana Bangel Marchaki, Laíza Rief Ruggeri, André Ferreira Hennigen, Thaís Muradás, Ana Beatriz Tittoni da Silveira, Elizandra Braganhol, Roselena Silvestri Schuh, Guilherme Baldo, Anelise Bergmann Araújo, Ana Helena Paz

{"title":"Effects of chorionic mesenchymal stromal cells, their conditioned medium, and membrane particles on neutrophil functionality.","authors":"Monique Maria Franco-da-Silva, Mariana Rauback Aubin, Alessandra Amaral de Vasconcellos, Dienifer Hermann Sirena, Giovana Bangel Marchaki, Laíza Rief Ruggeri, André Ferreira Hennigen, Thaís Muradás, Ana Beatriz Tittoni da Silveira, Elizandra Braganhol, Roselena Silvestri Schuh, Guilherme Baldo, Anelise Bergmann Araújo, Ana Helena Paz","doi":"10.1007/s00441-025-03970-6","DOIUrl":"https://doi.org/10.1007/s00441-025-03970-6","url":null,"abstract":"Mesenchymal stromal cells (MSC) are multipotent cells that can modulate immune cells, affecting macrophages, monocytes, and lymphocytes. Neutrophils are circulating leucocytes responsible for the first line of defense and can assume different phenotypes depending on their environment: N0, the naïve form, N1 (inflammatory), N2 (anti-inflammatory). This study explores the potentially protective roles of chorionic membrane MSCs and their products-conditioned medium and pre-conditioned cMSC-derived membrane microparticles (MP-cMSC)-on neutrophils. Conditioned medium treatment reduced the rate of apoptosis and enhanced the immunosuppressive potential consistent with an anti-inflammatory profile. MP-cMSC are a noteworthy cell-free therapy, consisting of artificially generated circular lipid bilayer structures with no cargo and approximately 200 nm in size. When added to neutrophil culture, MPs increased neutral red uptake, suggesting an enhanced phagocytic activity. In the MSC co-culture group, a reduced rate of apoptosis, increased neutral red uptake, and elevated programed death-ligand 1 (PD-L1) expression were observed. These findings suggest that the distinct effects elicited by conditioned media, microparticles, and co-culture are likely influenced by the specific nature of the interactions involved-whether purely paracrine, mediated through direct cell-to-cell contact, or a combination of both.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Long noncoding RNA XIST inhibition promotes Leydig cell apoptosis by acting as a competing endogenous RNA for microRNA‑145a‑5p that targets SIRT1 in late‑onset hypogonadism. 更正：长链非编码RNA XIST抑制通过作为microRNA - 145a - 5p的竞争内源性RNA促进间质细胞凋亡，microRNA - 145a - 5p在晚发性性腺功能减退中靶向SIRT1。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-03 DOI: 10.1007/s00441-025-03963-5

Jing Wang, Yiqiong Yang, Yang Xu, Zhipeng Xu, Xiaozhi Zhao, Ruipeng Jia, Yutian Dai

引用次数: 0

Retraction Note to: Preconditioning with melatonin improves therapeutic outcomes of bone marrow-derived mesenchymal stem cells in targeting liver fibrosis induced by CCl4. 注：褪黑素预处理可改善骨髓间充质干细胞靶向CCl4诱导的肝纤维化的治疗效果。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 DOI: 10.1007/s00441-025-03959-1

Keywan Mortezaee, Neda Khanlarkhani, Fatemeh Sabbaghziarani, Saeid Nekoonam, Jamal Majidpoor, Amir Hosseini, Parichehr Pasbakhsh, Iraj Ragerdi Kashani, Adib Zendedel

引用次数: 0

Gross anatomy of the visual processing centers of Hieroglyphus banian. 猴形猿视觉加工中心的大体解剖。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-02-12 DOI: 10.1007/s00441-025-03956-4

Sivaraju C, Joby Joseph

引用次数: 0