Cell and Tissue Research最新文献

Colonizing bacteria around aggregated lymphoid tissue of the rat ascending colon change diurnally and affect the host local transcriptome. 大鼠升结肠聚集淋巴组织周围的定殖细菌每天都在变化，并影响宿主的局部转录组。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-26 DOI: 10.1007/s00441-025-04000-1

Asaka Shimada, Naoto Kubota, Sika Zheng, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani

{"title":"Colonizing bacteria around aggregated lymphoid tissue of the rat ascending colon change diurnally and affect the host local transcriptome.","authors":"Asaka Shimada, Naoto Kubota, Sika Zheng, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani","doi":"10.1007/s00441-025-04000-1","DOIUrl":"10.1007/s00441-025-04000-1","url":null,"abstract":"The settlement levels of indigenous bacteria show circadian rhythms in various regions of the rat alimentary tract. Numerous bacteria colonize between the mucosal folds of the ascending colon in rodents; however, the rhythm of bacteria colonizing the ascending colon remains to be clarified. Therefore, we first aimed to examine the diurnal changes in bacteria colonizing in the rat ascending colon. The settlement levels of indigenous bacteria were significantly higher at zeitgeber time (ZT) 18 (dark phase) than at ZT6 (light phase) in the region encompassing the aggregated lymphoid tissue in the ascending colon (ALT-AC). The bacterial composition around the ALT-AC was dominated by the phylum Firmicutes and the family Lachnospiraceae, displaying notable distinctions from the compositions found in cecal contents and feces. The relative abundance of some bacterial species around the ALT-AC, such as Mucispirillum schaedleri, changed significantly between ZT6 and ZT18. Furthermore, we explored the effect of bacterial expansion on gene expression in the ALT-AC at ZT18 by administrating antibiotics for 1 day to inhibit bacterial growth. The antibiotic-treated group exhibited significant downregulation of multiple genes, including those associated with cell proliferation (Plk3), differentiation into goblet cells (Spdef, Atoh1, Bhlha15), and Golgi organization (Gorasp2). These results suggested that indigenous bacteria around the rat ALT-AC undergo diurnal changes in both settlement levels, peaking at the dark phase, and bacterial composition. In addition, bacterial expansion during the dark phase can induce changes in the expression of diverse genes, including genes associated with goblet cell differentiation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"97-108"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary cilium disappearance in podocytes during vertebrate phylogeny revealed by array tomography. 阵列断层扫描揭示脊椎动物系统发育过程中足细胞初级纤毛的消失。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-29 DOI: 10.1007/s00441-025-04002-z

Takayuki Miyaki, Jingyuan Xu, Makoto Sugiura, Hisako Kaneda, Juan Alejandro Oliva Trejo, Yuto Kawasaki, Takuya Omotehara, Takako Negishi-Koga, Muneaki Ishijima, Junji Yamaguchi, Soichiro Kakuta, Koichiro Ichimura

{"title":"Primary cilium disappearance in podocytes during vertebrate phylogeny revealed by array tomography.","authors":"Takayuki Miyaki, Jingyuan Xu, Makoto Sugiura, Hisako Kaneda, Juan Alejandro Oliva Trejo, Yuto Kawasaki, Takuya Omotehara, Takako Negishi-Koga, Muneaki Ishijima, Junji Yamaguchi, Soichiro Kakuta, Koichiro Ichimura","doi":"10.1007/s00441-025-04002-z","DOIUrl":"10.1007/s00441-025-04002-z","url":null,"abstract":"Podocytes are a type of epithelial cells that form the kidney nephron and are essential for ultrafiltration in the glomerulus. The majority of nephron constituent cells have primary cilia, which play an important role in the maintenance of normal tubular architecture of nephron. However, whether podocytes have primary cilia was only partially understood. In general, immunohistochemistry with an anti-acetylated α-tubulin antibody is often used to visualize primary cilia. α-Tubulin is highly acetylated throughout podocytes, and this antibody is not suitable to determine the presence or absence of primary cilia in podocytes, which is one reason why the presence of primary cilia in podocytes has remained unclear. In the present study, we determined the presence or absence of primary cilia in mature podocytes of six vertebrate species using a recently reported array tomography workflow optimized for whole glomerulus analysis. The proportion of podocytes with primary cilia tended to decrease with evolution as follows: 89.6% in river lampreys (Agnatha), 42.1% in zebrafish (Osteichthyes), 43.3% in African clawed frogs (Amphibia), 17.3% in Reeves' turtles (Reptilia), 10.4% in common quails (Aves), and 0.0% in Wistar rats (Mammalia). Our previous study has reported that, in rats, primary cilia are present in podocytes during development and disappear in mature podocytes. In other words, primary cilia disappear from podocytes during both phylogeny and ontogeny in vertebrates. We discuss the triggers and significance of primary cilium disappearance from vertebrate podocytes.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"51-63"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical relevance of the compartments and lymphocyte subsets in the human spleen. 人脾细胞间室和淋巴细胞亚群的临床相关性。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-29 DOI: 10.1007/s00441-025-04001-0

Reinhard Pabst

引用次数: 0

Functional role of circadian clock system in steroidogenesis and cell death pathways during corpus luteum regression in cattle. 生理时钟系统在黄体退化过程中甾体生成和细胞死亡途径中的功能作用。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-07-24 DOI: 10.1007/s00441-025-03997-9

Jing Zhang, Hanako Bai, Manabu Kawahara, Ahmed Z Balboula, Masashi Takahashi

{"title":"Functional role of circadian clock system in steroidogenesis and cell death pathways during corpus luteum regression in cattle.","authors":"Jing Zhang, Hanako Bai, Manabu Kawahara, Ahmed Z Balboula, Masashi Takahashi","doi":"10.1007/s00441-025-03997-9","DOIUrl":"10.1007/s00441-025-03997-9","url":null,"abstract":"The corpus luteum (CL) is an ovarian structure that secretes progesterone (P4) following ovulation, playing a crucial role in regulating the estrous cycle and maintaining pregnancy. Luteolysis, the structural and functional degradation of the CL, occurs through apoptosis and autophagy. Recent studies suggest that the circadian clock (CC) system, particularly the gene NR1D1, is involved in these processes. This study investigated the role of NR1D1 in bovine CL regression using an ex vivo model treated with prostaglandin F2α (PGF2α), the NR1D1 agonist GSK4112, and the antagonist SR8278. CL samples were classified into four estrous cycle stages based on ovarian morphology and analyzed for P4 secretion, as well as gene and protein expression related to steroid synthesis, the CC system, autophagy, and apoptosis. P4 levels, steroid synthesis-related genes, and CC system-related genes, including NR1D1, were highly expressed in the mid and late stages of the CL, whereas autophagy- and apoptosis-related genes peaked during regression. Western blotting and immunofluorescence revealed increased expression of NR1D1 and BMAL1 in the mid and late stages, while LC3 and CTSB were most prominent during regression. PGF2α treatment reduced NR1D1 and BMAL1 expression, along with decreased P4 levels and increased apoptosis markers. GSK4112 suppressed steroid synthesis while upregulating autophagy- and apoptosis-related genes. Conversely, SR8278 reversed PGF2α-induced luteal regression, restoring P4 and steroidogenic gene expression while suppressing CTSB. These findings suggest that NR1D1 interacts with PGF2α to regulate CL regression, highlighting the CC system as a potential target for improving reproductive efficiency in cattle.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"65-78"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanobody-based Pannexin1 channel inhibitors increase survival after cardiac ischemia/reperfusion. 基于纳米体的Pannexin1通道抑制剂增加心脏缺血/再灌注后的生存。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-07-23 DOI: 10.1007/s00441-025-03994-y

Olga M Rusiecka, Filippo Molica, Linda Clochard, Raf Van Campenhout, Timo W M De Groof, Viviane Bes, Nick Devoogdt, Serge Muyldermans, Mathieu Vinken, Brenda R Kwak

{"title":"Nanobody-based Pannexin1 channel inhibitors increase survival after cardiac ischemia/reperfusion.","authors":"Olga M Rusiecka, Filippo Molica, Linda Clochard, Raf Van Campenhout, Timo W M De Groof, Viviane Bes, Nick Devoogdt, Serge Muyldermans, Mathieu Vinken, Brenda R Kwak","doi":"10.1007/s00441-025-03994-y","DOIUrl":"10.1007/s00441-025-03994-y","url":null,"abstract":"Reperfusion following myocardial infarction salvages the ischemic heart but paradoxically exacerbates injury. Yet, efficient treatment for cardiac ischemia/reperfusion injury is still missing in clinics. ATP release through Pannexin1 (PANX1) channels facilitates recruitment of leukocytes to the injured myocardium. Thus, PANX1 channel inhibition might confer cardioprotection. Currently available PANX1 channel blockers lack specificity or in vivo stability. Nanobodies offer a new therapeutic modality given their high target affinity, small size, and deep tissue penetration. Nanobodies targeting Panx1 were recently introduced. Here, their target specificity and selective PANX1 channel inhibition for cardiovascular purposes were validated in vitro. The two most promising candidates were further examined in the context of cardiac ischemia/reperfusion injury. Nanobody-1 (Nb1) and Nb9 reduced neutrophil adhesion to an endothelial monolayer. Nb1 did not affect left ventricular function ex vivo; however, Nb9 tended to diminish the performance of isolated hearts. Finally, in vivo application of Nb1, but not of Nb9 or a control Nb, at the onset of reperfusion increased the survival rate of mice. However, the infarct size observed after treatment with Nb1 was similar than the one found after treatment with the control Nb. In conclusion, Nb1 efficiently and specifically inhibits ATP release from endothelial cells thereby limiting leukocyte adhesion and improving the outcome of cardiac ischemia/reperfusion in mice. This warrants further studies to unveil the detailed molecular mechanism underlying the beneficial effects of Nb1.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"121-137"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-derived growth factor signaling in porcine testicular tubular somatic cells. 猪睾丸小管体细胞的血小板衍生生长因子信号传导。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-21 DOI: 10.1007/s00441-025-04004-x

Nathalia de Lima E Martins Lara, Maryam Ahmadi Jeyhoonabadi, Rkia Dardari, Anja Elsenhans, Lin Su, Sadman Sakib, Ina Dobrinski

{"title":"Platelet-derived growth factor signaling in porcine testicular tubular somatic cells.","authors":"Nathalia de Lima E Martins Lara, Maryam Ahmadi Jeyhoonabadi, Rkia Dardari, Anja Elsenhans, Lin Su, Sadman Sakib, Ina Dobrinski","doi":"10.1007/s00441-025-04004-x","DOIUrl":"10.1007/s00441-025-04004-x","url":null,"abstract":"Mechanisms governing somatic cell interactions in the testis are not well defined. The platelet-derived growth factor (PDGF) pathway mediates epithelial-mesenchymal interactions and is involved in testicular morphogenesis in rodents. However, its roles in the testis of higher mammals remain largely unknown. Here, we investigated how PDGF signaling inhibition affects immature (1-week-old) porcine testicular tubular somatic cells (TTSCs), including cell-cell communication and morphogenesis. From scRNA-seq data, we established the PDGF pathway signatures in crosstalk between testicular cells, identifying Sertoli cells as the primary source and peritubular myoid cells as the main recipients of PDGF. Further, we demonstrated that PDGF inhibition by CP673451 affects TTSC functions, proliferation and cytoskeleton, with reduced cell area, focal adhesion size and fibronectin production, and decreased expression of peritubular myoid cell-specific genes. PDGF inhibition did not impair testicular organoid formation and tubule morphogenesis in vitro, but it correlated with ablation of cytoplasmic extensions from the tubule surface, potentially related to interactions between TTSCs and the extracellular matrix. PDGF signaling can be transduced by primary cilia, sensory organelles that respond to environmental stimuli, and PDGF inhibition increased the percentage of ciliated cells and ciliary length in TTSCs. Comprehensive morphological characterization of primary cilia in the porcine testis indicated that these remain submerged in the cytoplasm. In conclusion, the PDGF signaling pathway is active in the immature pig testis and may influence testis morphogenesis by affecting cell-extracellular matrix interaction, cytoskeleton and primary cilia. However, the role of primary cilia-modulated PDGF signaling in the testis remains to be determined.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"79-96"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZO-2 is a scaffold at the centriole and mitotic spindle poles that enhances microtubule stability and supports the proper development of mitotic spindles and cilia. ZO-2是中心粒和有丝分裂纺锤体两极的支架，增强微管稳定性，支持有丝分裂纺锤体和纤毛的正常发育。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-07-29 DOI: 10.1007/s00441-025-03992-0

Sara Vega-Torreblanca, Diana Cristina Pinto-Dueñas, Christian Hernández-Guzmán, Dolores Martín-Tapia, Lourdes Alarcón, Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Sirenia González-Pozos, Josué David Hernández-Varela, Leticia Ramírez-Martínez, Esther López-Bayghen, José Jorge Chanona-Pérez, Lorenza González-Mariscal

{"title":"ZO-2 is a scaffold at the centriole and mitotic spindle poles that enhances microtubule stability and supports the proper development of mitotic spindles and cilia.","authors":"Sara Vega-Torreblanca, Diana Cristina Pinto-Dueñas, Christian Hernández-Guzmán, Dolores Martín-Tapia, Lourdes Alarcón, Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Sirenia González-Pozos, Josué David Hernández-Varela, Leticia Ramírez-Martínez, Esther López-Bayghen, José Jorge Chanona-Pérez, Lorenza González-Mariscal","doi":"10.1007/s00441-025-03992-0","DOIUrl":"10.1007/s00441-025-03992-0","url":null,"abstract":"Previous studies revealed the presence of several tight junction (TJ) proteins in the centrosome and their interaction with various centriolar proteins, prompting us to analyze whether this also applies to the TJ protein ZO-2. Here, we found that ZO-2 colocalizes with CEP164 in the distal appendage of the mother centriole and is also present in the pericentriolar region, mitotic spindle poles, the basal body of primary cilia, and the tail of spermatozoa. The absence of ZO-2 altered the cellular content of centriolar proteins CEP164, centriolin, and CEP135, but did not change the morphology of centrioles. ZO-2 depletion inhibits the development of astral and mitotic spindle microtubules expressing EB1. At the spindle poles, ZO-2 depletion increases the accumulation of NuMA while reducing the levels of kinesin KIF14 and the TPX2 scaffold, and the accumulation of the kinase p-Aurora, leading to a decrease in mitotic spindle length, microtubule instability, and abnormal chromosome congression. KIF14, NuMA, and p-Aurora co-immunoprecipitate with ZO-2, and NuMA and Aurora-A bind to different segments of ZO-2. At the ciliary basal body, ZO-2 depletion reduces the content of CEP164, KIF14, and IFT-B protein IFT57, while increasing the expression of p-Aurora and pAKT. These changes block primary cilium development and the response to Sonic Hedgehog signaling pathway stimulation. These results suggest that, rather than being a centrosomal architectural component, ZO-2 enhances microtubule stability and serves as a scaffold that facilitates the adequate accumulation of spindle pole and centriole proteins, allowing proper poleward spindle microtubule flux and cilia development.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"21-50"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression of villin and advillin by neuroendocrine and tuft cells in the murine lower airways. 绒毛蛋白和肾上腺素在小鼠下气道神经内分泌和簇状细胞中的差异表达。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-09-01 DOI: 10.1007/s00441-025-04003-y

Wafaa Mahmoud, Alexander Perniss, Krupali Poharkar, Maryam Keshavarz, Ulrich Gärtner, Johannes Oberwinkler, Burkhard Schütz, Thomas Worzfeld, Stefan Offermanns, Wolfgang Kummer

{"title":"Differential expression of villin and advillin by neuroendocrine and tuft cells in the murine lower airways.","authors":"Wafaa Mahmoud, Alexander Perniss, Krupali Poharkar, Maryam Keshavarz, Ulrich Gärtner, Johannes Oberwinkler, Burkhard Schütz, Thomas Worzfeld, Stefan Offermanns, Wolfgang Kummer","doi":"10.1007/s00441-025-04003-y","DOIUrl":"10.1007/s00441-025-04003-y","url":null,"abstract":"Previous studies identified a rare cell type in the mouse tracheal epithelium with immunoreactivity to the microvillus protein villin (Vil1), which persisted in mice lacking tuft cells due to deletion of the transcription factor Pou2f3. This study aimed to clarify the identity of this ill-defined cell type. Ultrastructurally, all cells with tightly packed microvilli observed in the tracheal epithelium of Pou2f3-/--mice contained basally located dense core vesicles, a characteristic feature of neuroendocrine cells (NEC). Accordingly, immunofluorescence double-labeling utilizing NEC markers revealed villin-labeling in two thirds of NEC in the trachea, a reporter mouse strain showed Cre recombinase activity driven by the Vil1 promoter in a subpopulation of tracheal NEC, and analysis of single cell RNA sequencing data revealed Vil1-mRNA expression by tracheal NEC. Notably, only a minimal fraction (≈1%) of bronchopulmonary NEC (solitary and clustered in neuroepithelial bodies) displayed villin-immunoreactivity, despite nearly half of them having a history of Vil1 promoter activity. Microvilli of tuft cells differed ultrastructurally from those of NEC, and the majority of tuft cells were immunoreactive to advillin (Avil), showed Avil promoter activity as indicated by a reporter mouse strain, and expressed Avil-mRNA in the sequencing data set. This study uncovers villin-expressing cells in the lower airways as a cell population hidden among NEC. Advillin, not villin, is identified as a marker for airway tuft cells. This should be considered in interpreting findings based on the use of villin as a marker or Cre-driver when investigating rare cells in the murine airways.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"1-20"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-29b targets ADAM12 and 19 to regulate the extracellular matrix in lamina cribrosa cells. MicroRNA-29b靶向ADAM12和adam19调控筛板细胞的细胞外基质。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-22 DOI: 10.1007/s00441-025-04006-9

Aoife Smyth, Breedge Callaghan, Mustapha Irnaten, Darrell Andrews, Colin E Willoughby, Colm O'Brien

{"title":"MicroRNA-29b targets ADAM12 and 19 to regulate the extracellular matrix in lamina cribrosa cells.","authors":"Aoife Smyth, Breedge Callaghan, Mustapha Irnaten, Darrell Andrews, Colin E Willoughby, Colm O'Brien","doi":"10.1007/s00441-025-04006-9","DOIUrl":"https://doi.org/10.1007/s00441-025-04006-9","url":null,"abstract":"Glaucoma remains the leading cause of irreversible blindness worldwide, with elevated intraocular pressure (IOP) being the only modifiable risk factor in primary open-angle glaucoma (POAG). Despite adequate IOP control, many patients continue to progress to irreversible optic neuropathy, emphasising the need for alternate treatments. Transforming growth factor-beta (TGF-β) promotes extracellular matrix (ECM) production and fibrosis at the optic nerve head (ONH) in glaucoma. A disintegrin and metalloprotease-12 and metalloprotease-19 (ADAM12 and ADAM19) are implicated in fibrosis. Recent studies have explored miRNA-based manipulation of the TGF-β signalling pathway as a potential therapeutic strategy in fibrosis. This study investigates whether miR-29b modulation affects ADAM12, ADAM19, and ECM gene expression in human lamina cribrosa (LC) cells. Primary human normal lamina cribrosa (NLC) and glaucoma LC (GLC) cells were treated with TGF-β1 and transfected with either a miR-29b mimic or control. Gene expression levels of ADAM12, ADAM19, miR-29b, and several ECM genes were quantified using real-time RT-qPCR, and protein expression levels by Western blotting. ADAM12 and ADAM19 expression was elevated in untreated GLC cells, and treatment with TGF-β1 in both NLC and GLC cells increased ADAM12 and ADAM19 expression. The expression of miR-29b was significantly reduced in both GLC- and TGF-β1-treated NLC and GLC cells. Transfection with miR-29b resulted in a marked reduction in ADAM12 and ADAM19 mRNA expression in TGF-β1-treated NLC and GLC cells. Additionally, miR-29b transfection reduced ECM gene expression in both NLC and GLC under TGF-β1 stimulation. Our results demonstrate that miR-29b plays a crucial role in fibrotic remodelling at the LC by antagonising the effects of TGF-β1 on ADAM and ECM gene expression, representing a novel therapeutic target in glaucoma.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental changes in the Aedes aegypti mosquito endocrine gland complex. 埃及伊蚊内分泌腺复合体的发育变化。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-22 DOI: 10.1007/s00441-025-04012-x

Lenka Rouhová, Gabriela Krejčová, Ana Beatriz Barletta Ferreira, Houda Ouns Maaroufi, Fernando G Noriega, Carolina Barillas-Mury, Hana Sehadová, Marcela Nouzova

{"title":"Developmental changes in the Aedes aegypti mosquito endocrine gland complex.","authors":"Lenka Rouhová, Gabriela Krejčová, Ana Beatriz Barletta Ferreira, Houda Ouns Maaroufi, Fernando G Noriega, Carolina Barillas-Mury, Hana Sehadová, Marcela Nouzova","doi":"10.1007/s00441-025-04012-x","DOIUrl":"https://doi.org/10.1007/s00441-025-04012-x","url":null,"abstract":"In the larvae of the mosquito Aedes aegypti, the three most important endocrine glands, the corpora allata (CA), the corpora cardiaca (CC), and the prothoracic gland (PG), together form the glandular complexes (GC). Using confocal laser scanning microscopy in combination with immunohistochemistry, in situ hybridization, ultrastructural expansion microscopy, and apoptosis studies, we were able to identify the different cell types of the GC and follow their fate during metamorphosis. Our studies revealed that the CC is not a well-defined organ but consists of individual cells randomly distributed within the GC and CA-CC complexes. Furthermore, imaging and in situ hybridization show that the CA is a compact organ composed of a single cell type. We observed that CA and CC survive during the larval-to-adult transition, while PG undergoes apoptosis and disappears within the first 24 h of adult life. This study lays the foundation for a more detailed understanding of the structure and changes in the major endocrine organs of mosquitoes, which are vectors of several important infectious diseases.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0