Cell and Tissue Research最新文献

Disruption of Myd88 in a salmonid epithelioid cell line reveals its contribution to bacterial detection and immune response. 在鲑鱼上皮样细胞系中破坏Myd88揭示了其对细菌检测和免疫反应的贡献。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-23 DOI: 10.1007/s00441-025-04015-8

Alexander Rebl, Mathilde Peruzzi, Catherine Collins, Niccolò Vendramin, Pierre Boudinot, Niels Lorenzen, Bertrand Collet

{"title":"Disruption of Myd88 in a salmonid epithelioid cell line reveals its contribution to bacterial detection and immune response.","authors":"Alexander Rebl, Mathilde Peruzzi, Catherine Collins, Niccolò Vendramin, Pierre Boudinot, Niels Lorenzen, Bertrand Collet","doi":"10.1007/s00441-025-04015-8","DOIUrl":"https://doi.org/10.1007/s00441-025-04015-8","url":null,"abstract":"Toll-like receptors (TLRs) are a class of pattern-recognition receptors that recognize pathogen- and damage-associated molecular patterns and initiate immune responses. TLRs selectively recruit distinct adapter molecules such as the myeloid differentiation primary response protein 88 (MyD88) that mediates signaling downstream of all TLRs, with the exception of TLR3. To investigate TLR signaling pathways in fish, we engineered a knockout clonal epitheliod fish cell line, named MYD88C2, using CRISPR/Cas9-mediated genome editing to disrupt the myd88 gene. We characterized the phenotype of this cell line alongside a wild-type cell line through gene-expression profiling and reporter-gene analyses in the context of stimulation with heat-killed Vibrio anguillarum, heat-killed Escherichia coli, flagellin, zymosan, and inoculation with a panel of viruses. We demonstrate that the proinflammatory response to zymosan and flagellin, as measured through the induction of proinflammatory genes, was greatly reduced in the MYD88C2 cell line. The responsiveness to zymosan was found to be partially restored by transfecting the MYD88C2 cell line with a myd88-expression plasmid. In contrast, the loss of the myd88 gene had no impact on the cytopathic effect associated with the replication of viral haemorrhagic septicemia virus (VHSV), infectious haematopoietic necrosis virus (IHNV), infectious pancreatic necrosis virus (IPNV), spring viraemia of carp virus (SVCV) or infectious salmon anaemia virus (ISAV). These findings highlight the critical role of MyD88 in mediating specific proinflammatory responses to bacterial and fungal stimuli, while its absence has no detectable impact on viral replication or cytopathogenicity in epithelioid fish cells under the conditions tested.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D reconstruction of the ovariole in Nilaparvata lugens (Hemiptera: Delphacidae) by FIB-SEM. 用FIB-SEM对褐飞虱卵巢三维重建（半翅目：褐飞虱科）

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-22 DOI: 10.1007/s00441-025-04017-6

Ke-Qi Ye, Guan Wang, Jian-Sheng Guo, Chuan-Xi Zhang, Xiao-Ping Yu, Dan-Ting Li

{"title":"3D reconstruction of the ovariole in Nilaparvata lugens (Hemiptera: Delphacidae) by FIB-SEM.","authors":"Ke-Qi Ye, Guan Wang, Jian-Sheng Guo, Chuan-Xi Zhang, Xiao-Ping Yu, Dan-Ting Li","doi":"10.1007/s00441-025-04017-6","DOIUrl":"https://doi.org/10.1007/s00441-025-04017-6","url":null,"abstract":"The brown planthopper (Nilaparvata lugens) has a high reproductive rate, posing a significant challenge to biological control of rice pests. This is largely due to the physiological and functional traits of its telotrophic ovarioles, which feature a centralized nutrient supply to ensure each oocyte obtains essential basic nutrients. Current studies on insect ovarioles primarily focus on hormonal regulation and gene expression; the cellular composition and spatial relationships within N. lugens ovariole tissues remain poorly understood. To illustrate the internal architecture of ovarioles and the intricate oogenesis process at nanoscale resolution, this study employed focused ion beam-scanning electron microscopy for 3D volume reconstruction, overcoming limitations of traditional 2D electron microscopy. Using this advanced imaging technique, we systematically characterized key ovariole components including terminal filaments, trophocytes, germ cell clusters, three types of follicular cells, nutritive cords, and oocytes. Our findings reveal that rather than maintaining a continuous linkage, the nutritive cord transiently connects to the oocytes during specific developmental stages to facilitate nutrient transfer. An assembly of unique, cake-like phospholipoglycoproteins was identified within the oocytes, suggesting specialized energy storage mechanisms. The detailed 3D model elucidates the spatial relationship and relative position between various parts of the ovariole, offering novel insights into the mechanisms of oocyte growth and nutrient acquisition. These findings advance foundational knowledge of insect reproductive biology and provide a valuable framework for future research on pest control strategies targeting N. lugens reproduction.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LOXHD1b knockout alters swimming behavior in zebrafish. 敲除LOXHD1b改变斑马鱼的游泳行为。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-10 DOI: 10.1007/s00441-025-04005-w

Yoichi Asaoka, Shunsuke Tarumoto, Yoshinobu Hirose, Aya Iwamoto, Masayuki Tokunaga, Takafumi Matsuura, Yosuke Takemoto, Hiroshi Yamashita, Makoto Furutani-Seiki, Kazuma Sugahara

{"title":"LOXHD1b knockout alters swimming behavior in zebrafish.","authors":"Yoichi Asaoka, Shunsuke Tarumoto, Yoshinobu Hirose, Aya Iwamoto, Masayuki Tokunaga, Takafumi Matsuura, Yosuke Takemoto, Hiroshi Yamashita, Makoto Furutani-Seiki, Kazuma Sugahara","doi":"10.1007/s00441-025-04005-w","DOIUrl":"https://doi.org/10.1007/s00441-025-04005-w","url":null,"abstract":"Since human inner ear hair cells do not regenerate, the current treatments of hereditary deafness depend on hearing aids or cochlear implant. However, uncovering the functions of genes responsible for hereditary hearing loss is not only useful for their diagnosis but also for developing therapies. The pathogenetic mechanism of human non-syndromic deafness DFNB77 without morphological defects in the inner year caused by LOXHD1 mutations is not fully understood. We introduced zebrafish because the lateral line hair cells are structurally and physiologically similar to the human inner ear hair cells and mutations involved in non-symptomatic hearing loss can be assessed by their swimming behavior. The knock-out (KO) of LOXHD1b gene which is expressed in the lateral line hair cells was generated using the CRISPR-Cas9 system in zebrafish, and its morphological and functional changes were evaluated. As with human patients the LOXHD1b KO zebrafish larvae did not exhibit detectable morphological defects, but showed prolonged water flow sensing time. These results suggest that LOXHD1b plays pivotal roles for the hair cell neural activity and its KO zebrafish mutant serves as a useful model for revealing the molecular mechanisms linking LOXHD with hair cell function and for a drug screening to rescue the swimming phenotype.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phagophores originate from endoplasmic reticulum membranes in vasopressin neurons in a mouse model of familial neurohypophysial diabetes insipidus. 家族性神经垂体性尿崩症小鼠模型中抗利尿激素神经元的吞噬细胞起源于内质网膜。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-04 DOI: 10.1007/s00441-025-04013-w

Takashi Miyata, Daisuke Hagiwara, Ryosei Ashida, Satoshi Naito, Yohei Kawaguchi, Tomoko Handa, Tomoko Kobayashi, Mariko Sugiyama, Takeshi Onoue, Shintaro Iwama, Hidetaka Suga, Ryoichi Banno, Mami Matsumoto, Hidetoshi Urakubo, Nobuhiko Ohno, Hiroshi Arima

{"title":"Phagophores originate from endoplasmic reticulum membranes in vasopressin neurons in a mouse model of familial neurohypophysial diabetes insipidus.","authors":"Takashi Miyata, Daisuke Hagiwara, Ryosei Ashida, Satoshi Naito, Yohei Kawaguchi, Tomoko Handa, Tomoko Kobayashi, Mariko Sugiyama, Takeshi Onoue, Shintaro Iwama, Hidetaka Suga, Ryoichi Banno, Mami Matsumoto, Hidetoshi Urakubo, Nobuhiko Ohno, Hiroshi Arima","doi":"10.1007/s00441-025-04013-w","DOIUrl":"https://doi.org/10.1007/s00441-025-04013-w","url":null,"abstract":"Familial neurohypophysial diabetes insipidus (FNDI) is an autosomal dominant disorder caused by mutations in the arginine vasopressin (AVP) gene. In AVP neurons in a mouse model of FNDI, aggregates of mutant AVP precursors accumulate within a specific compartment of the endoplasmic reticulum (ER). However, as FNDI mice aged, or were exposed to repeated water deprivation, the ER lumen dilated and mutant aggregates dispersed throughout the ER. Meanwhile, autophagic isolation membranes, known as phagophores, emerged to envelop ER containing these aggregates, indicating induction of ER-phagy. Previous in vitro studies showed that phagophores originate from ER membranes, but the structural relationship between phagophores and the ER membrane in vivo remains unknown. In this study, we used serial block-face scanning electron microscopy to investigate the structural relationship between phagophores, ER membranes, and protein aggregates within dilated ER of AVP neurons from FNDI mice subjected to intermittent water deprivation for 4 weeks. Three-dimensional analysis revealed that phagophores enveloped aggregates located within the dilated ER. Serial imaging further demonstrated a physical connection between these phagophores and intact ER membranes. This study provides the first in vivo evidence of the structural continuity between phagophores and the ER membrane in AVP neurons in a mouse model of FNDI.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colonizing bacteria around aggregated lymphoid tissue of the rat ascending colon change diurnally and affect the host local transcriptome. 大鼠升结肠聚集淋巴组织周围的定殖细菌每天都在变化，并影响宿主的局部转录组。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-26 DOI: 10.1007/s00441-025-04000-1

Asaka Shimada, Naoto Kubota, Sika Zheng, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani

{"title":"Colonizing bacteria around aggregated lymphoid tissue of the rat ascending colon change diurnally and affect the host local transcriptome.","authors":"Asaka Shimada, Naoto Kubota, Sika Zheng, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani","doi":"10.1007/s00441-025-04000-1","DOIUrl":"10.1007/s00441-025-04000-1","url":null,"abstract":"The settlement levels of indigenous bacteria show circadian rhythms in various regions of the rat alimentary tract. Numerous bacteria colonize between the mucosal folds of the ascending colon in rodents; however, the rhythm of bacteria colonizing the ascending colon remains to be clarified. Therefore, we first aimed to examine the diurnal changes in bacteria colonizing in the rat ascending colon. The settlement levels of indigenous bacteria were significantly higher at zeitgeber time (ZT) 18 (dark phase) than at ZT6 (light phase) in the region encompassing the aggregated lymphoid tissue in the ascending colon (ALT-AC). The bacterial composition around the ALT-AC was dominated by the phylum Firmicutes and the family Lachnospiraceae, displaying notable distinctions from the compositions found in cecal contents and feces. The relative abundance of some bacterial species around the ALT-AC, such as Mucispirillum schaedleri, changed significantly between ZT6 and ZT18. Furthermore, we explored the effect of bacterial expansion on gene expression in the ALT-AC at ZT18 by administrating antibiotics for 1 day to inhibit bacterial growth. The antibiotic-treated group exhibited significant downregulation of multiple genes, including those associated with cell proliferation (Plk3), differentiation into goblet cells (Spdef, Atoh1, Bhlha15), and Golgi organization (Gorasp2). These results suggested that indigenous bacteria around the rat ALT-AC undergo diurnal changes in both settlement levels, peaking at the dark phase, and bacterial composition. In addition, bacterial expansion during the dark phase can induce changes in the expression of diverse genes, including genes associated with goblet cell differentiation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"97-108"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary cilium disappearance in podocytes during vertebrate phylogeny revealed by array tomography. 阵列断层扫描揭示脊椎动物系统发育过程中足细胞初级纤毛的消失。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-29 DOI: 10.1007/s00441-025-04002-z

Takayuki Miyaki, Jingyuan Xu, Makoto Sugiura, Hisako Kaneda, Juan Alejandro Oliva Trejo, Yuto Kawasaki, Takuya Omotehara, Takako Negishi-Koga, Muneaki Ishijima, Junji Yamaguchi, Soichiro Kakuta, Koichiro Ichimura

{"title":"Primary cilium disappearance in podocytes during vertebrate phylogeny revealed by array tomography.","authors":"Takayuki Miyaki, Jingyuan Xu, Makoto Sugiura, Hisako Kaneda, Juan Alejandro Oliva Trejo, Yuto Kawasaki, Takuya Omotehara, Takako Negishi-Koga, Muneaki Ishijima, Junji Yamaguchi, Soichiro Kakuta, Koichiro Ichimura","doi":"10.1007/s00441-025-04002-z","DOIUrl":"10.1007/s00441-025-04002-z","url":null,"abstract":"Podocytes are a type of epithelial cells that form the kidney nephron and are essential for ultrafiltration in the glomerulus. The majority of nephron constituent cells have primary cilia, which play an important role in the maintenance of normal tubular architecture of nephron. However, whether podocytes have primary cilia was only partially understood. In general, immunohistochemistry with an anti-acetylated α-tubulin antibody is often used to visualize primary cilia. α-Tubulin is highly acetylated throughout podocytes, and this antibody is not suitable to determine the presence or absence of primary cilia in podocytes, which is one reason why the presence of primary cilia in podocytes has remained unclear. In the present study, we determined the presence or absence of primary cilia in mature podocytes of six vertebrate species using a recently reported array tomography workflow optimized for whole glomerulus analysis. The proportion of podocytes with primary cilia tended to decrease with evolution as follows: 89.6% in river lampreys (Agnatha), 42.1% in zebrafish (Osteichthyes), 43.3% in African clawed frogs (Amphibia), 17.3% in Reeves' turtles (Reptilia), 10.4% in common quails (Aves), and 0.0% in Wistar rats (Mammalia). Our previous study has reported that, in rats, primary cilia are present in podocytes during development and disappear in mature podocytes. In other words, primary cilia disappear from podocytes during both phylogeny and ontogeny in vertebrates. We discuss the triggers and significance of primary cilium disappearance from vertebrate podocytes.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"51-63"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical relevance of the compartments and lymphocyte subsets in the human spleen. 人脾细胞间室和淋巴细胞亚群的临床相关性。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-29 DOI: 10.1007/s00441-025-04001-0

Reinhard Pabst

引用次数: 0

Functional role of circadian clock system in steroidogenesis and cell death pathways during corpus luteum regression in cattle. 生理时钟系统在黄体退化过程中甾体生成和细胞死亡途径中的功能作用。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-07-24 DOI: 10.1007/s00441-025-03997-9

Jing Zhang, Hanako Bai, Manabu Kawahara, Ahmed Z Balboula, Masashi Takahashi

{"title":"Functional role of circadian clock system in steroidogenesis and cell death pathways during corpus luteum regression in cattle.","authors":"Jing Zhang, Hanako Bai, Manabu Kawahara, Ahmed Z Balboula, Masashi Takahashi","doi":"10.1007/s00441-025-03997-9","DOIUrl":"10.1007/s00441-025-03997-9","url":null,"abstract":"The corpus luteum (CL) is an ovarian structure that secretes progesterone (P4) following ovulation, playing a crucial role in regulating the estrous cycle and maintaining pregnancy. Luteolysis, the structural and functional degradation of the CL, occurs through apoptosis and autophagy. Recent studies suggest that the circadian clock (CC) system, particularly the gene NR1D1, is involved in these processes. This study investigated the role of NR1D1 in bovine CL regression using an ex vivo model treated with prostaglandin F2α (PGF2α), the NR1D1 agonist GSK4112, and the antagonist SR8278. CL samples were classified into four estrous cycle stages based on ovarian morphology and analyzed for P4 secretion, as well as gene and protein expression related to steroid synthesis, the CC system, autophagy, and apoptosis. P4 levels, steroid synthesis-related genes, and CC system-related genes, including NR1D1, were highly expressed in the mid and late stages of the CL, whereas autophagy- and apoptosis-related genes peaked during regression. Western blotting and immunofluorescence revealed increased expression of NR1D1 and BMAL1 in the mid and late stages, while LC3 and CTSB were most prominent during regression. PGF2α treatment reduced NR1D1 and BMAL1 expression, along with decreased P4 levels and increased apoptosis markers. GSK4112 suppressed steroid synthesis while upregulating autophagy- and apoptosis-related genes. Conversely, SR8278 reversed PGF2α-induced luteal regression, restoring P4 and steroidogenic gene expression while suppressing CTSB. These findings suggest that NR1D1 interacts with PGF2α to regulate CL regression, highlighting the CC system as a potential target for improving reproductive efficiency in cattle.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"65-78"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanobody-based Pannexin1 channel inhibitors increase survival after cardiac ischemia/reperfusion. 基于纳米体的Pannexin1通道抑制剂增加心脏缺血/再灌注后的生存。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-07-23 DOI: 10.1007/s00441-025-03994-y

Olga M Rusiecka, Filippo Molica, Linda Clochard, Raf Van Campenhout, Timo W M De Groof, Viviane Bes, Nick Devoogdt, Serge Muyldermans, Mathieu Vinken, Brenda R Kwak

{"title":"Nanobody-based Pannexin1 channel inhibitors increase survival after cardiac ischemia/reperfusion.","authors":"Olga M Rusiecka, Filippo Molica, Linda Clochard, Raf Van Campenhout, Timo W M De Groof, Viviane Bes, Nick Devoogdt, Serge Muyldermans, Mathieu Vinken, Brenda R Kwak","doi":"10.1007/s00441-025-03994-y","DOIUrl":"10.1007/s00441-025-03994-y","url":null,"abstract":"Reperfusion following myocardial infarction salvages the ischemic heart but paradoxically exacerbates injury. Yet, efficient treatment for cardiac ischemia/reperfusion injury is still missing in clinics. ATP release through Pannexin1 (PANX1) channels facilitates recruitment of leukocytes to the injured myocardium. Thus, PANX1 channel inhibition might confer cardioprotection. Currently available PANX1 channel blockers lack specificity or in vivo stability. Nanobodies offer a new therapeutic modality given their high target affinity, small size, and deep tissue penetration. Nanobodies targeting Panx1 were recently introduced. Here, their target specificity and selective PANX1 channel inhibition for cardiovascular purposes were validated in vitro. The two most promising candidates were further examined in the context of cardiac ischemia/reperfusion injury. Nanobody-1 (Nb1) and Nb9 reduced neutrophil adhesion to an endothelial monolayer. Nb1 did not affect left ventricular function ex vivo; however, Nb9 tended to diminish the performance of isolated hearts. Finally, in vivo application of Nb1, but not of Nb9 or a control Nb, at the onset of reperfusion increased the survival rate of mice. However, the infarct size observed after treatment with Nb1 was similar than the one found after treatment with the control Nb. In conclusion, Nb1 efficiently and specifically inhibits ATP release from endothelial cells thereby limiting leukocyte adhesion and improving the outcome of cardiac ischemia/reperfusion in mice. This warrants further studies to unveil the detailed molecular mechanism underlying the beneficial effects of Nb1.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"121-137"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-derived growth factor signaling in porcine testicular tubular somatic cells. 猪睾丸小管体细胞的血小板衍生生长因子信号传导。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-21 DOI: 10.1007/s00441-025-04004-x

Nathalia de Lima E Martins Lara, Maryam Ahmadi Jeyhoonabadi, Rkia Dardari, Anja Elsenhans, Lin Su, Sadman Sakib, Ina Dobrinski

{"title":"Platelet-derived growth factor signaling in porcine testicular tubular somatic cells.","authors":"Nathalia de Lima E Martins Lara, Maryam Ahmadi Jeyhoonabadi, Rkia Dardari, Anja Elsenhans, Lin Su, Sadman Sakib, Ina Dobrinski","doi":"10.1007/s00441-025-04004-x","DOIUrl":"10.1007/s00441-025-04004-x","url":null,"abstract":"Mechanisms governing somatic cell interactions in the testis are not well defined. The platelet-derived growth factor (PDGF) pathway mediates epithelial-mesenchymal interactions and is involved in testicular morphogenesis in rodents. However, its roles in the testis of higher mammals remain largely unknown. Here, we investigated how PDGF signaling inhibition affects immature (1-week-old) porcine testicular tubular somatic cells (TTSCs), including cell-cell communication and morphogenesis. From scRNA-seq data, we established the PDGF pathway signatures in crosstalk between testicular cells, identifying Sertoli cells as the primary source and peritubular myoid cells as the main recipients of PDGF. Further, we demonstrated that PDGF inhibition by CP673451 affects TTSC functions, proliferation and cytoskeleton, with reduced cell area, focal adhesion size and fibronectin production, and decreased expression of peritubular myoid cell-specific genes. PDGF inhibition did not impair testicular organoid formation and tubule morphogenesis in vitro, but it correlated with ablation of cytoplasmic extensions from the tubule surface, potentially related to interactions between TTSCs and the extracellular matrix. PDGF signaling can be transduced by primary cilia, sensory organelles that respond to environmental stimuli, and PDGF inhibition increased the percentage of ciliated cells and ciliary length in TTSCs. Comprehensive morphological characterization of primary cilia in the porcine testis indicated that these remain submerged in the cytoplasm. In conclusion, the PDGF signaling pathway is active in the immature pig testis and may influence testis morphogenesis by affecting cell-extracellular matrix interaction, cytoskeleton and primary cilia. However, the role of primary cilia-modulated PDGF signaling in the testis remains to be determined.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"79-96"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0