Cell and Tissue Research最新文献

Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-10 DOI: 10.1007/s00441-025-03955-5

Kaaj Pala, Kevin Xueying Sun, Lars Krogvold, Knut Dahl-Jørgensen, Shiva Reddy

{"title":"Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease.","authors":"Kaaj Pala, Kevin Xueying Sun, Lars Krogvold, Knut Dahl-Jørgensen, Shiva Reddy","doi":"10.1007/s00441-025-03955-5","DOIUrl":"https://doi.org/10.1007/s00441-025-03955-5","url":null,"abstract":"During type 1 diabetes (T1D), oxidative stress in beta cells may cause early beta cell dysfunction and initiate autoimmunity. Mouse islets express lower levels of reactive oxygen species (ROS) clearing enzymes, such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase than several other tissues. It remains unclear if human beta cells show a similar deficiency during T1D or exhibit a higher degree of intrinsic resistance to oxidative stress. We compared islet cell distributions and determined graded intensities of glutathione peroxidase1 (GPX1), a key enzymatic mediator involved in detoxifying hydrogen peroxide, by applying combined immunohistochemistry for GPX1, insulin and glucagon, in pancreatic sections from new-onset T1D (group 1), non-diabetic autoantibody-negative (group 2), non-diabetic autoantibody-positive (group 3) and long-term diabetic (group 4) donors. Islets from all study groups demonstrated either uniform but graded staining intensities for GPX1 in almost all islet cells or strong staining in selective islet cells with weaker intensities in the remaining cells. GPX1 was present in selective glucagon cells and insulin cells, including in cells negative for both hormones, with stronger intensities in a higher percentage of glucagon than insulin cells. It was absent in a higher percentage of beta cells than glucagon cells independent of disease or autoantibody positivity. We conclude that a proportion of human beta cells and glucagon cells express GPX1 but show heterogeneity in its distribution and intensities, independent of disease or autoantibody status. Our studies highlight important differences in the expression of GPX1 in islet cell-types between mice and humans.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-05 DOI: 10.1007/s00441-025-03960-8

Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov

{"title":"Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.","authors":"Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov","doi":"10.1007/s00441-025-03960-8","DOIUrl":"https://doi.org/10.1007/s00441-025-03960-8","url":null,"abstract":"Microtubules are the principal cytoskeletal component in cells, integral to various morphogenetic processes in Metazoa, including cell migration, adhesion, and polarity. Their dynamics and functions are modulated by tubulin post-translational modifications (PTMs). While studies on model species have provided insights into microtubule functions, understanding their evolutionary aspects necessitates exploring non-model organisms. Sponges (phylum Porifera) are an early-branching metazoan group with outstanding regenerative capacities. This research presents the first comprehensive analysis of microtubule organization and tubulin PTMs in calcareous sponges. The intact sponge cells show various but typical types of microtubule organization, while detected tubulin PTMs are associated with certain cell types, indicating specific functions in particular cellular contexts. During regeneration, relying on the coordinated movement of epithelial-like cell sheets, microtubule networks in exopinacocytes and choanocytes undergo significant reorganization. These rearranged microtubules potentially stabilize cellular migration direction and facilitate cargo transport, essential for cell contact and polarity establishment. This study enhances our understanding of microtubule functionality and regulation in early-diverging metazoans, contributing to the broader evolutionary context of cytoskeletal dynamics.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DjsoxP-1 and Djsox5 are essential for tissue homeostasis and regeneration in Dugesia japonica. DjsoxP-1和Djsox5对杜鹃花的组织稳态和再生至关重要。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1007/s00441-024-03939-x

Weiyun Guo, Xiao Liu, Lina Pang, Zhihong Kong, Ziyi Lin, Jing Ren, Zimei Dong, Guangwen Chen, Dezeng Liu

{"title":"DjsoxP-1 and Djsox5 are essential for tissue homeostasis and regeneration in Dugesia japonica.","authors":"Weiyun Guo, Xiao Liu, Lina Pang, Zhihong Kong, Ziyi Lin, Jing Ren, Zimei Dong, Guangwen Chen, Dezeng Liu","doi":"10.1007/s00441-024-03939-x","DOIUrl":"https://doi.org/10.1007/s00441-024-03939-x","url":null,"abstract":"Sox genes encode a family of transcription factors that regulate multiple biological processes during metazoan development, including embryogenesis, tissue homeostasis, nervous system specification, and stem cell maintenance. The planarian Dugesia japonica contains a reservoir of stem cells that grow and divide continuously to support cellular turnover. However, whether SOX proteins retain these conserved functions in planarians remains to be determined. In this study, three sox gene homologs, DjsoxP-1, DjsoxP-5, and Djsox5, were identified in the planarian transcriptome, and their roles were investigated. The results showed that the amino acids deduced from the three sox genes all contained high-mobility group (HMG) domain sequences, which are highly conserved in sox family members. Whole-mount in situ hybridization (WISH) and real-time quantitative PCR (RT-qPCR) results indicated that the three sox genes were mainly expressed in parenchymal tissues and regenerative blastema. Additionally, X-ray irradiation assay and dFISH suggested that the three Djsox genes were expressed in neoblasts and other cell types. Head regression in intact planarian and smaller blastemas in both head or tail fragments of regenerating planarians were exhibited with DjsoxP-1 and Djsox5 RNA interference (RNAi) compared to the control animals, suggesting that DjsoxP-1 and Djsox5 have essential roles during cellular turnover and regeneration in planarians; conversely, there was no obvious phenotypic abnormalities or regeneration defect in DjsoxP-5 RNAi animals. Knockdown of DjsoxP-1 or Djsox5 decreased neoblast proliferation and promoted cell apoptosis. In conclusion, our findings demonstrate that DjsoxP-1 and Djsox5 are involved in cellular turnover and regeneration in planarians by modulating coordination between cell proliferation and apoptosis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"399 3","pages":"337-350"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics. 大鼠垂体中间叶的CD9/ sox2阳性细胞表现出间充质干细胞的特征。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-14 DOI: 10.1007/s00441-024-03947-x

Ayano Shindo, Morio Azuma, Ken Fujiwara, Saishu Yoshida, Kotaro Horiguchi

{"title":"CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics.","authors":"Ayano Shindo, Morio Azuma, Ken Fujiwara, Saishu Yoshida, Kotaro Horiguchi","doi":"10.1007/s00441-024-03947-x","DOIUrl":"10.1007/s00441-024-03947-x","url":null,"abstract":"Adult tissue stem cells of the anterior pituitary gland, CD9/SOX2-positive cells, are believed to exist in the marginal cell layer (MCL) bordering the residual lumen of the Rathke's pouch. These cells migrate from the intermediate lobe side of the MCL (IL-MCL) to the anterior lobe side of the MCL and may be involved in supplying hormone-producing cells. Previous studies reported that some SOX2-positive cells of the anterior lobe differentiate into skeletal muscle cells. These findings suggest that CD9/SOX2-positive cells in the anterior pituitary have mesenchymal stem cell (MSC) properties. To substantiate this hypothesis, we examined whether CD9-positive cells isolated from IL-MCL of adult male rats differentiate into mesenchymal cells, such as endothelial cells, adipocytes, chondrocytes, and osteocytes. Immunohistochemical analysis revealed that the CD9-positive cells were positive for the MSC markers, CD349, CD105, CD271, and CD273 and were detected in the early postnatal period at the boundary between the posterior and intermediate lobes but not in the embryonic period. In addition, some adult tissue stem cells derived from neural crest cells and bone marrow haematopoietic stem cells were positive for both CD9 and MSC markers, indicating that several CD9/SOX2-positive cells in the IL-MCL of the pituitary gland are MSCs that invaded from external tissues during pituitary development in the early postnatal period and exist in the adult tissue stem cells as suppliers of hormone-producing and endothelial cells in the anterior lobe. These findings should have implications for the application of CD9/SOX2-positive cells in regenerative therapy of the pituitary.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"277-290"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis. 淡水小龙虾造血组织的组织与表达分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-04 DOI: 10.1007/s00441-024-03943-1

Thanapong Kruangkum, Kenneth Söderhäll, Irene Söderhäll

{"title":"The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis.","authors":"Thanapong Kruangkum, Kenneth Söderhäll, Irene Söderhäll","doi":"10.1007/s00441-024-03943-1","DOIUrl":"10.1007/s00441-024-03943-1","url":null,"abstract":"The hematopoietic tissue (HPT) and anterior proliferation center (APC) are the main hemocyte-producing organs of the freshwater crayfish, Pacifastacus leniusculus. To deepen our understanding of immune responses to various pathogens, it is essential to identify distinct hemocyte subpopulations with specific functions and to further explore how these cells are generated. Here we provide an in-depth histological study of the HPT and APC in order to localize cell types in different developmental stages, and to provide some information regarding the hemocyte differentiation in the crayfish. We localized mRNA expression of previously identified genes in the HPT/APC and hemocytes by RNA-FISH. The expression of hemolectin and transglutaminase 1 was shown to be co-localized in a high number of the HPT cells, while transglutaminase 2 was expressed in different cell types mainly associated with epithelium or endothelium. Furthermore, by double RNA-FISH for hemolectin and a previously unidentified PDGF-like factor, combined with immunostaining for prophenoloxidase, we could identify several different subtypes of hemocytes, indicating that the immune function of hemocytes in crayfish is more diversified and complex than previously appreciated.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"303-322"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and histochemical characteristics of the foregut, midgut, and hindgut, and their alterations during ovarian development in female freshwater prawn, Macrobrachium rosenbergii. 罗氏沼虾雌虾前、中、后肠形态和组织化学特征及其在卵巢发育过程中的变化。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-13 DOI: 10.1007/s00441-024-03948-w

Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul

{"title":"Morphological and histochemical characteristics of the foregut, midgut, and hindgut, and their alterations during ovarian development in female freshwater prawn, Macrobrachium rosenbergii.","authors":"Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul","doi":"10.1007/s00441-024-03948-w","DOIUrl":"10.1007/s00441-024-03948-w","url":null,"abstract":"The anatomical, histological, and histochemical characteristics of the foregut (FG), midgut (MG), and hindgut (HG), as well as their alterations during the ovarian cycle in female prawns, Macrobrachium rosenbergii, were investigated. The esophagus (ESO), cardia (CD), and pylorus (PY) are the main components of the FG. An epithelium (Ep) with thick cuticle (Cu) layers lining the ESO, and the ESO is encircled by the ESO glands. The CD has a thick musculature, whereas the Ep of the PY are characterized by numerous villi and columnar Ep cells with a thinner layer of Cu. The inner longitudinal (LM) and the outer circular (CM) muscles were both present in the PY. The MG is lined by Ep cells which are connected to the basement membrane, and it lacks Cu. Microvilli, and subapical vacuoles are visible on the apical surface of Ep cells of the MG. The outermost layer is characterized by a dense strip of elastic fibers and a cluster of collagen fibers. The HG has the Ep cells with a thin Cu layer, and the HG glands form a rosette-like structure. The HG is surrounded by the CM and the LM fibers. The reactivities of Periodic Acid Schiff and Alcian Blue in these digestive organs altered throughout the ovarian cycle, and this was supported by the increased expression of mucin levels as ovarian maturation progressed. Our results offer novel and significant insights into the anatomical and histochemical structures of these digestive organs, and demonstrate a significant correlation between ovarian development and feeding in the female prawn, M. rosenbergii.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"351-375"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic memory as crucial contributing factor in directing the differentiation of human iPSC into pancreatic β-cells in vitro.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-30 DOI: 10.1007/s00441-025-03952-8

Abdoulaye Diane, Razik Bin Abdul Mu-U-Min, Heba Hussain Al-Siddiqi

{"title":"Epigenetic memory as crucial contributing factor in directing the differentiation of human iPSC into pancreatic β-cells in vitro.","authors":"Abdoulaye Diane, Razik Bin Abdul Mu-U-Min, Heba Hussain Al-Siddiqi","doi":"10.1007/s00441-025-03952-8","DOIUrl":"10.1007/s00441-025-03952-8","url":null,"abstract":"Impaired insulin secretion contributes to the pathogenesis of type 1 diabetes mellitus through autoimmune destruction of pancreatic β-cells and the pathogenesis of severe forms of type 2 diabetes mellitus through β-cell dedifferentiation and other mechanisms. Replenishment of malfunctioning β-cells via islet transplantation has the potential to induce long-term glycemic control in the body. However, this treatment option cannot widely be implemented in clinical due to healthy islet donor shortage. Emerging β-cell replacement with human-induced pluripotent stem cell (iPSC) provides high remedial therapy hopes. Thus, tremendous progress has been made in developing β-cell differentiation protocols in vitro; however, most of the differentiated iPSC-derived β-cells showed immature phenotypes associated with low efficiency depending on the iPSC lines used, creating a crucial barrier for their clinical implementation. Multiple mechanisms including differences in genetic, cell cycle patterns, and mitochondrial dysfunction underlie the defective differentiation propensity of iPSC into insulin-producing β-cells. Accumulating evidence recently indicated that, following the reprogramming, epigenetic memory inherited from parental cells substantially affects the differentiation capacity of many iPSC lines. Therefore, differences in epigenetic signature are likely to be essential contributing factors influencing the propensity of iPSC differentiation. In this review, we will document the impact of the epigenome on the reprogramming efficacy and differentiation potential of iPSCs and how targeting the epigenetic residual memory could be an additional strategy to improve the differentiation efficiency of existing protocols to generate fully functional hPSC-derived pancreatic β-cells for diabetes therapy and drug screening.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"267-276"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles support increased expansion of mesenchymal stromal cells on fetal membrane-derived decellularized extracellular matrix. 细胞外囊泡支持胚胎膜来源的脱细胞细胞外基质上间充质间质细胞的增加。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2024-12-24 DOI: 10.1007/s00441-024-03946-y

Aida Shakouri-Motlagh, Andrea J O'Connor, Shaun P Brennecke, Daniel E Heath, Bill Kalionis

{"title":"Extracellular vesicles support increased expansion of mesenchymal stromal cells on fetal membrane-derived decellularized extracellular matrix.","authors":"Aida Shakouri-Motlagh, Andrea J O'Connor, Shaun P Brennecke, Daniel E Heath, Bill Kalionis","doi":"10.1007/s00441-024-03946-y","DOIUrl":"10.1007/s00441-024-03946-y","url":null,"abstract":"Decidual mesenchymal stromal cells (DMSC) were the source of extracellular vesicles (DMSC_EV). The xCELLigence real-time cell growth assay revealed increasing concentrations of EVs decreased DMSC attachment in the early growth phase but stimulated DMSC proliferation at day 7 when grown on tissue culture plastic (TCP). DMSC attachment and proliferation varied depending on the growth surface and DMSC_EV supplementation. DMSC attachment increased on decellularized and solubilized amniotic (s-dAM) whether or not EVs were added. Only Matrigel substrate increased DMSC attachment with added EVs. The addition of EVs increased DMSC proliferation only on the s-dAM substrate. DMSCs were more motile on s-dAM and decellularized and solubilized chorionic (s-dCM) membranes following EV addition. The osteogenic potential of DMSCs was improved on s-dAM substrates when supplanted with EVs. Finally, the levels of reactive oxygen species in DMSCs varied depending on the substrate but not on added EVs. We show that the addition of in vitro EVs isolated from the source being expanded (i.e., DMSCs) and the presence of ECM improve DMSC behaviours during ex vivo expansion. The inclusion of two key components of the MSC niche, EVs and ECM, benefitted the ex vivo expansion of MSCs. Added in vitro EVs increased the proliferation of DMSCs when grown on s-dAM but not on s-dCM, whereas they improved DMSC mobility on both surfaces. Testing different ECMs could be used to promote specific desired characteristics of DMSCs, and different combinations of EVs and ECM may enhance desirable MSC characteristics for specific therapeutic settings.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"323-336"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform. 使用Xenium平台对人类骨骼肌进行原位空间转录组学分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-09 DOI: 10.1007/s00441-024-03945-z

Nejc Umek, Marija Meznarič, Žiga Šink, Kaja Blagotinšek Cokan, Uršula Prosenc Zmrzljak, Simon Horvat

{"title":"In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform.","authors":"Nejc Umek, Marija Meznarič, Žiga Šink, Kaja Blagotinšek Cokan, Uršula Prosenc Zmrzljak, Simon Horvat","doi":"10.1007/s00441-024-03945-z","DOIUrl":"10.1007/s00441-024-03945-z","url":null,"abstract":"Traditional transcriptomic studies often overlook the complex heterogeneity of skeletal muscle, as they typically isolate RNA from mixed muscle fibre and cell populations, resulting in an averaged transcriptomic profile that obscures fibre type-specific differences. This study assessed the potential of the recently developed Xenium platform for high-resolution spatial transcriptomic analysis of human skeletal muscle histological sections. Human vastus lateralis muscle samples from two individuals were analysed using the Xenium platform and Human Multi-Tissue and Cancer Panel targeting 377 genes complemented by staining of successive sections for Myosin Heavy Chain isoforms to differentiate between type 1 and type 2 muscle fibres. Manual segmentation of muscle fibres allowed accurate comparisons of transcript densities across fibre types and subcellular regions, overcoming limitations in the platform's automated segmentation. The analysis revealed higher transcript density in type 1 fibres, particularly in nuclear and perinuclear areas, and identified 191 out of 377 genes with differential expression between muscle fibres and perimysium. Genes such as PROX1, S100A1, LGR5, ACTA2, and LPL exhibited higher expression in type 1 fibres, whereas PEBP4, CAVIN1, GATM, and PVALB in type 2 fibres. We demonstrated that the Xenium platform is capable of high-resolution spatial in situ transcriptomic analysis of skeletal muscle histological sections. This study demonstrates that, with manual segmentation, the Xenium platform effectively performs fibre type-specific transcriptomic analysis, providing new insights into skeletal muscle biology.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"291-302"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recruitment of pulmonary intravascular macrophages in SARS-CoV-2 infected hamsters.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-27 DOI: 10.1007/s00441-025-03958-2

Carolina Rego Rodrigues, Gurpreet Kaur Aulakh, Andrea Kroeker, Swarali S Kulkarni, Jocelyne Lew, Darryl Falzarano, Baljit Singh

{"title":"Recruitment of pulmonary intravascular macrophages in SARS-CoV-2 infected hamsters.","authors":"Carolina Rego Rodrigues, Gurpreet Kaur Aulakh, Andrea Kroeker, Swarali S Kulkarni, Jocelyne Lew, Darryl Falzarano, Baljit Singh","doi":"10.1007/s00441-025-03958-2","DOIUrl":"https://doi.org/10.1007/s00441-025-03958-2","url":null,"abstract":"The mechanisms by which severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe lung inflammation and mortality remain unclear. While the role of alveolar macrophages in COVID-19 is known, data on pulmonary intravascular macrophages (PIMs) is lacking. PIMs are key inflammatory cells present in species like cattle and pigs. Though constitutively absent in humans and rodents, their recruitment in rodents triggers exaggerated inflammation. We investigated the recruitment of PIMs and other immune cells, using immunofluorescence, hematoxylin and eosin (H&E) staining, and immunogold labeling in a hamster model of SARS-CoV-2 infection. Syrian golden hamsters were divided into 6 groups: uninfected control, unvaccinated-infected at 2-, 5-, and 14-days post infection (dpi) and vaccinated-infected at 5- and 14-dpi. Lung tissues were analyzed for neutrophils (myeloperoxidase), monocytes/macrophages (CCR2, CX3CR1), macrophages (IBA-1), and T cells (CD3). Septal macrophages increased at 2-, 5-, and 14-dpi in infected animals vs. control. CX3CR1 + cells decreased at 14-dpi in unvaccinated animals, but CX3CR1/CCR2 double positive cells were higher at 5-dpi, indicating a pro-inflammatory macrophage phenotype. PIMs were confirmed by transmission electron microscopy. These are the first data showing recruitment of pro-inflammatory PIMs in SARS-CoV-2 infected lungs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0