Cell and Tissue Research最新文献

Proteomic analysis of secreted proteins derived from amniotic fluid stem cells. 羊水干细胞分泌蛋白的蛋白质组学分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-07 DOI: 10.1007/s00441-025-03984-0

Tatsanee Phermthai, Puttachart Chuaynarong, Suparat Wichitwiengrat, Kamonpat Phermthai, Sittiruk Roytrakul, Thanuch Chitthira, Sasiprapa Thongbopit

{"title":"Proteomic analysis of secreted proteins derived from amniotic fluid stem cells.","authors":"Tatsanee Phermthai, Puttachart Chuaynarong, Suparat Wichitwiengrat, Kamonpat Phermthai, Sittiruk Roytrakul, Thanuch Chitthira, Sasiprapa Thongbopit","doi":"10.1007/s00441-025-03984-0","DOIUrl":"https://doi.org/10.1007/s00441-025-03984-0","url":null,"abstract":"Mesenchymal stem cells (MSCs) show promising therapeutic effects due to the proteins they secrete. However, MSCs from different sources exhibit only 60% similarity of proteins they secrete, suggesting that unique proteins may offer distinct therapeutic properties based on their origin. Amniotic fluid-derived MSCs (AFSCs) are promising for treating degenerative diseases and are unique in providing sufficient cells for fetal therapies. Nevertheless, their proteomic profiles remain largely undefined. This study investigated the proteomic profiles of bioactive molecules secreted by AFSCs (AFSC-se) using liquid chromatography and mass spectrometry, along with bioinformatics tools for protein function analysis. We identified over 2000 proteins in the AFSC-se that are involved in various mechanisms supporting organ development and function. The top three proteins identified were associated with organelle fusion, forebrain morphogenesis, and response to parathyroid hormone. Our findings indicate that AFSC-se has the ability to inhibit inflammation and apoptosis, which corresponds to 7.8% of the identified proteins involved in pathways related to these therapeutic effects. Furthermore, we discovered that 20% of identified proteins are associated with brain functions including synaptogenesis, neurogenesis, and neuroprotection. In conclusion, the proteomic profile of AFSC-se indicates its potential therapeutic effects. The significant presence of neuro-related proteins in AFSC-se suggests that AFSC-se may be a promising candidate for treating neurological diseases. Our work addresses existing knowledge gaps in this field.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hsa-MiR-483 -3p regulates the extracellular matrix proteins via TGFβ2/SMAD4 signaling in the glucocorticoid-responsive human trabecular meshwork cells. 在糖皮质激素应答的人小梁网细胞中，Hsa-MiR-483 -3p通过tgf - β2/SMAD4信号通路调节细胞外基质蛋白。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-05 DOI: 10.1007/s00441-025-03985-z

Ravinarayanan Haribalaganesh, Rajendrababu Sharmila, Ramasamy Krishnadas, Colin E Willoughby, Srinivasan Senthilkumari

{"title":"Hsa-MiR-483 -3p regulates the extracellular matrix proteins via TGFβ2/SMAD4 signaling in the glucocorticoid-responsive human trabecular meshwork cells.","authors":"Ravinarayanan Haribalaganesh, Rajendrababu Sharmila, Ramasamy Krishnadas, Colin E Willoughby, Srinivasan Senthilkumari","doi":"10.1007/s00441-025-03985-z","DOIUrl":"https://doi.org/10.1007/s00441-025-03985-z","url":null,"abstract":"The purpose of this study was to investigate the role of hsa-miR-483-3p on the regulation of extracellular matrix (ECM) in cultured human trabecular meshwork (HTM) cells with known steroid response. Primary cultures of HTM cells with known steroid responsiveness [GC-responder (GC-R) and GC-Non-responder (GC-NR) cells] were grown on coverslip in 12-well plate until 80% confluence and treated with 100 nM dexamethasone (DEX) for 24 h and transfected with different concentrations of synthetic miRNA 483-3p mimic or inhibitor. After 24 h or 72 h post transfection, the cells were harvested for the following experiments: (i) percentage transfection efficiency, (ii) RNA isolation for qPCR analysis, (iii) immunofluorescence staining, and (iv) protein isolation for Western blotting, respectively. All experiments were performed in triplicate with three biological samples (n = 3). GC-R HTM cells showed significantly higher expression of SMAD4 as compared to GC-NR HTM cells. Similarly, DEX treatment up-regulated the SMAD4-dependent ECM proteins. The presence of a miR-483-3p mimic down-regulated SMAD4 expression and SMAD4-dependent ECM production in a dose-dependent manner by negatively down-regulating SMAD4/TGF-β2 signaling. The inhibition of SMAD4-dependent ECM production by miR-483-3p was more pronounced in GC-R HTM cells as compared to GC-NR cells. The down-regulation in ECM production by miR-483-3p is SMAD4-dependent and may play a protective role in mitigating steroid response in GC-R HTM cells. Using miR-483-3p mimics demonstrates therapeutic potential for the management of steroid-induced ocular hypertension and glaucoma.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Local inflammation at the salmon louse (Lepeophtheirus salmonis) attachment site contributes to copepodid rejection in coho salmon (Oncorhynchus kisutch). 鲑鱼虱（Lepeophtheirus salmonis）附着部位的局部炎症有助于鲑（Oncorhynchus kisutch）的桡足类排斥反应。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-04 DOI: 10.1007/s00441-025-03976-0

Lene Sveen, Mark D Fast, Torstein Tengs, Rachel A Kline, Judit Aguilar Marti, Dominic Kurian, Gerrit Timmerhaus, Marianne Vaadal, Ross D Houston, James E Bron, Sean J Monaghan, Haitham H Mohammed, Rose Ruiz Daniels, Sarah Salisbury, Diego Robledo, Mark Braceland, Miroslava Hansen, Nicholas Robinson

{"title":"Local inflammation at the salmon louse (Lepeophtheirus salmonis) attachment site contributes to copepodid rejection in coho salmon (Oncorhynchus kisutch).","authors":"Lene Sveen, Mark D Fast, Torstein Tengs, Rachel A Kline, Judit Aguilar Marti, Dominic Kurian, Gerrit Timmerhaus, Marianne Vaadal, Ross D Houston, James E Bron, Sean J Monaghan, Haitham H Mohammed, Rose Ruiz Daniels, Sarah Salisbury, Diego Robledo, Mark Braceland, Miroslava Hansen, Nicholas Robinson","doi":"10.1007/s00441-025-03976-0","DOIUrl":"https://doi.org/10.1007/s00441-025-03976-0","url":null,"abstract":"The study investigates the susceptibility of Atlantic salmon (Salmo salar) and Pacific salmon species (pink salmon, Oncorhynchus gorbuscha; coho salmon, Oncorhynchus kisutch; and chum salmon, Oncorhynchus keta) to the parasitic salmon lice (Lepeophtheirus salmonis). The research had two main objectives: to characterize the morphology of the scaly skin in four salmonid species and to compare the cellular response at the louse attachment site in coho salmon and Atlantic salmon. Three consecutive challenge trials were conducted, with successful louse infestation only achieved across all four species in the third trial using mild anesthesia with tricaine methanesulfonate. Skin and fin samples were collected at 12, 24, 36, 48, 60, and 168 h post-infestation (hpi) for histological, proteomic, and spatial transcriptomic analyses. Results showed that chum salmon had significantly higher mucous cell coverage (30-40%) in the epithelium of scaly skin compared to Atlantic salmon (10%). At the louse attachment site in coho salmon, there was a greater influx of inflammatory cells at 36-48 hpi compared to Atlantic salmon. Proteomic analysis at 12 hpi and 36 hpi in coho salmon showed upregulation of neutrophil degranulation and formyl-methionyl-leucyl-phenylalanine signaling. Additionally, spatial transcriptomics at the attachment site showed local upregulation of inflammatory gene markers. These findings suggest that coho salmon mount a rapid and large-scale inflammatory response driven by neutrophils to louse attachment within the first 48 hpi. Overall, the study emphasizes the significance of local changes at the host-parasite interface for resistance mechanisms against salmon lice.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The large milkweed bugs' Na,K-ATPase β-subunits colocalize with septate junction proteins in a tissue-specific manner. 大型乳草虫的Na， k - atp酶β-亚基以组织特异性的方式与分离的连接蛋白共定位。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-26 DOI: 10.1007/s00441-025-03965-3

Marlena Herbertz, Christian Lohr, Susanne Dobler

{"title":"The large milkweed bugs' Na,K-ATPase β-subunits colocalize with septate junction proteins in a tissue-specific manner.","authors":"Marlena Herbertz, Christian Lohr, Susanne Dobler","doi":"10.1007/s00441-025-03965-3","DOIUrl":"10.1007/s00441-025-03965-3","url":null,"abstract":"The Na,K-ATPase is a vital transmembrane enzyme, which is important for maintaining cell membrane potentials and the general functionality of animal cells. The enzyme's minimal functional unit consists of one α and one β-subunit, whereas the number of existing paralogs varies in different insect species. The functional roles of different β-subunits, which can account for their diversity within a single species, are so far only partially explained. The emphasis of this study was to specifically elucidate the involvement in septate junctions of the four β-subunits of the new model system Oncopeltus fasciatus. Septate junctions function as a paracellular barrier controlling the flow of solutes across epithelia. So far, studies in Drosophila revealed that nervana2, the β2 homolog of Drosophila, is involved in septate junction formation. In O. fasciatus, we demonstrate that most of the Na,K-ATPase subunits colocalize with septate junction proteins. This agrees with our previous findings implying a role of β2 in the control of tracheal tube size in O. fasciatus, which according to the findings in Drosophila appears to be dependent on a stable formation of septate junctions. Finally, our data suggest a connection between the septate junction protein coracle and the enigmatic, N-terminally strongly truncated βx, which has no obvious homologs in other insects. Our study proposes that the four β-subunits form functional units with septate junction proteins, either allowing tissue-adjusted formation of cell-cell contacts or other yet unknown functions.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"347-363"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term consumption of moderate amounts of sucrose-sweetened drinks disrupts intestinal barrier function by impairing goblet cell differentiation. 长期饮用适量的含糖饮料会损害杯状细胞的分化，从而破坏肠道屏障功能。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-12 DOI: 10.1007/s00441-025-03961-7

Sachiko Sato, Arif U Hasan, Mami Obara, Yukiko Kondo, Eiichi Taira

{"title":"Long-term consumption of moderate amounts of sucrose-sweetened drinks disrupts intestinal barrier function by impairing goblet cell differentiation.","authors":"Sachiko Sato, Arif U Hasan, Mami Obara, Yukiko Kondo, Eiichi Taira","doi":"10.1007/s00441-025-03961-7","DOIUrl":"10.1007/s00441-025-03961-7","url":null,"abstract":"While the prolonged consumption of sucrose-containing beverages is known to impact many organs, their specific effects on the small intestine remain elusive. This study aimed to evaluate how regular intake of sucrose, in amounts typically consumed, affects goblet cells, which play a critical role in regulating the mucosal barrier and innate immune defenses in the small intestine. Ten-week-old male ddY mice, a model of diet-induced obesity, were given a regular diet with either plain water or 7% sucrose water. Caloric intake was monitored weekly through food and drink measurements. After 8 weeks, glucose and insulin responses were evaluated following an oral gavage of glucose or sucrose. At 14 weeks, plasma, whole small intestine, and liver samples were collected. Despite achieving an isocaloric state, mice drinking sucrose water showed approximately a 1.5-fold increase in body weight and impaired glucose tolerance. In the small intestine, genes involved in sucrose digestion and absorption (Sis, Sglt1, Glut2, and Glut5) were upregulated, while genes essential for maintaining the intestinal barrier and function (Epcam, Fabp2, Cldn1, Ocln, and Tjp1) were downregulated. Serum levels and mRNA expression of the inflammatory cytokine, interleukin-18 were elevated. Genes responsible for goblet cell differentiation and function (Hes1, Gfi1, Spdef, and Klf4) were downregulated, leading to an increase in immature goblet cells and a decrease in mucin-producing markers (Muc2, Muc4, and Muc13) in the jejunum. The findings underscore that besides obesity, long-term intake of sucrose-containing drinks provokes localized inflammation and disrupts small intestinal barrier function by impairing goblet cell differentiation and activity.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"273-285"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease. 1型糖尿病供者和有无胰岛细胞自身抗体的非糖尿病供者胰岛中谷胱甘肽过氧化物酶-1免疫反应细胞的分布是可变的，与疾病无关。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-10 DOI: 10.1007/s00441-025-03955-5

Kaaj Pala, Kevin Xueying Sun, Lars Krogvold, Knut Dahl-Jørgensen, Shiva Reddy

{"title":"Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease.","authors":"Kaaj Pala, Kevin Xueying Sun, Lars Krogvold, Knut Dahl-Jørgensen, Shiva Reddy","doi":"10.1007/s00441-025-03955-5","DOIUrl":"10.1007/s00441-025-03955-5","url":null,"abstract":"During type 1 diabetes (T1D), oxidative stress in beta cells may cause early beta cell dysfunction and initiate autoimmunity. Mouse islets express lower levels of reactive oxygen species (ROS) clearing enzymes, such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase than several other tissues. It remains unclear if human beta cells show a similar deficiency during T1D or exhibit a higher degree of intrinsic resistance to oxidative stress. We compared islet cell distributions and determined graded intensities of glutathione peroxidase1 (GPX1), a key enzymatic mediator involved in detoxifying hydrogen peroxide, by applying combined immunohistochemistry for GPX1, insulin and glucagon, in pancreatic sections from new-onset T1D (group 1), non-diabetic autoantibody-negative (group 2), non-diabetic autoantibody-positive (group 3) and long-term diabetic (group 4) donors. Islets from all study groups demonstrated either uniform but graded staining intensities for GPX1 in almost all islet cells or strong staining in selective islet cells with weaker intensities in the remaining cells. GPX1 was present in selective glucagon cells and insulin cells, including in cells negative for both hormones, with stronger intensities in a higher percentage of glucagon than insulin cells. It was absent in a higher percentage of beta cells than glucagon cells independent of disease or autoantibody positivity. We conclude that a proportion of human beta cells and glucagon cells express GPX1 but show heterogeneity in its distribution and intensities, independent of disease or autoantibody status. Our studies highlight important differences in the expression of GPX1 in islet cell-types between mice and humans.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"255-271"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of glutamate-related disease-dependent alterations in subventricular NSCs of the 3xTg Alzheimer's disease model, could they be involved in attempting damage repair? 鉴定3xTg阿尔茨海默病模型脑室下NSCs中谷氨酸相关疾病依赖性改变，它们是否可能参与损伤修复？

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-02-17 DOI: 10.1007/s00441-025-03954-6

Giorgia Cerqueni, Valentina Terenzi, Alessandra Preziuso, Tiziano Serfilippi, Silvia Piccirillo, Mariangela Di Vincenzo, Patrizia Ambrogini, Salvatore Amoroso, Monia Orciani, Vincenzo Lariccia, Simona Magi

{"title":"Identification of glutamate-related disease-dependent alterations in subventricular NSCs of the 3xTg Alzheimer's disease model, could they be involved in attempting damage repair?","authors":"Giorgia Cerqueni, Valentina Terenzi, Alessandra Preziuso, Tiziano Serfilippi, Silvia Piccirillo, Mariangela Di Vincenzo, Patrizia Ambrogini, Salvatore Amoroso, Monia Orciani, Vincenzo Lariccia, Simona Magi","doi":"10.1007/s00441-025-03954-6","DOIUrl":"10.1007/s00441-025-03954-6","url":null,"abstract":"Alzheimer's disease (AD) is an age-related neurodegenerative disorder characterised by several factors, such as impaired glutamate neurotransmission affecting crucial functions. Neural stem cells (NSCs) are present in the adult brains of all mammalian species and contribute to the continuous generation of neural cells throughout life. The disruption of glutamate levels during the development of AD could impact NSCs' functionality, influencing their response to the microenvironment. In this work, we isolated adult neural stem cells from both triple transgenic (3xTg)-AD mice and age-matched wild type (WT) mice in order to gather information on any differences between them, particularly concerning the potential mechanisms involved in the internalisation of glutamate and its utilisation for energy production. The 3xTg model offers the ability to recapitulate human pathology with both plaque and tangle hallmarks that are involved in the process of glutamate release. In vitro culture 3xTg NSCs showed a slight morphological difference compared to WT cells and a massive reduction of proliferation and viability. Furthermore, 3xTg NSCs displayed an increase in the expression of glutamate transporters and glutamine synthetase, while glutamate dehydrogenase did not show any reduction, which is typical in AD brains. Data obtained from this basic research study suggest a possible involvement of glutamate in the cellular energy balance, indicating an attempted response of NSCs to the cytotoxic microenvironment in the early stage of AD pathology. This finding is of great interest, as it corroborates the hypothesis that targeting the glutamatergic system could be an extremely promising strategy for new therapeutics in AD.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"241-253"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges. 钙质海绵中完整组织和再生过程中的微管组织和微管蛋白翻译后修饰。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-05 DOI: 10.1007/s00441-025-03960-8

Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov

{"title":"Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.","authors":"Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov","doi":"10.1007/s00441-025-03960-8","DOIUrl":"10.1007/s00441-025-03960-8","url":null,"abstract":"Microtubules are the principal cytoskeletal component in cells, integral to various morphogenetic processes in Metazoa, including cell migration, adhesion, and polarity. Their dynamics and functions are modulated by tubulin post-translational modifications (PTMs). While studies on model species have provided insights into microtubule functions, understanding their evolutionary aspects necessitates exploring non-model organisms. Sponges (phylum Porifera) are an early-branching metazoan group with outstanding regenerative capacities. This research presents the first comprehensive analysis of microtubule organization and tubulin PTMs in calcareous sponges. The intact sponge cells show various but typical types of microtubule organization, while detected tubulin PTMs are associated with certain cell types, indicating specific functions in particular cellular contexts. During regeneration, relying on the coordinated movement of epithelial-like cell sheets, microtubule networks in exopinacocytes and choanocytes undergo significant reorganization. These rearranged microtubules potentially stabilize cellular migration direction and facilitate cargo transport, essential for cell contact and polarity establishment. This study enhances our understanding of microtubule functionality and regulation in early-diverging metazoans, contributing to the broader evolutionary context of cytoskeletal dynamics.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"365-381"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of hydroxyapatite nanoparticles on the cellular processes of stem cells derived from dental tissue sources. 羟基磷灰石纳米颗粒对牙组织来源干细胞细胞过程的影响。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-18 DOI: 10.1007/s00441-025-03962-6

Mais Emad, Mohammad Alnatour, Walhan Alshaer, Jennifer L Gibbs, Benoît Michot, Dana Alqudah, Alaa A A Aljabali, Mairvat Al-Mrahleh, Abdolelah Jaradat, Duaa Abuarqoub

{"title":"Impact of hydroxyapatite nanoparticles on the cellular processes of stem cells derived from dental tissue sources.","authors":"Mais Emad, Mohammad Alnatour, Walhan Alshaer, Jennifer L Gibbs, Benoît Michot, Dana Alqudah, Alaa A A Aljabali, Mairvat Al-Mrahleh, Abdolelah Jaradat, Duaa Abuarqoub","doi":"10.1007/s00441-025-03962-6","DOIUrl":"10.1007/s00441-025-03962-6","url":null,"abstract":"Hydroxyapatite nanoparticle (HANPs) utilization has recently been notable in bone tissue engineering. This surge owes itself to the biocompatibility of HANPs and their striking resemblance to the minerals found in natural bone. Furthermore, dental pulp-derived stem cells (DPSCs) have garnered attention due to their remarkable differentiation potential into multilineages, thus positioning them as a pivotal cell reservoir for regenerative medicine. This study aims to investigate the impact of HANPs on DPSCs cellular processes. The HANPs have been synthesized using the wet chemical precipitation method followed by freeze-drying and characterization using dynamic light scattering (DLS) and transmission electron microscopy (TEM). The size of HANPs was reported to be in the range of 55-67 nm. Our dataset divulges that DPSCs can endure concentrations of HANPs up to ≤ 0.81 mg/mL without incurring any conspicuous alterations in their morphology or the pace of proliferation. Furthermore, the self-renewal potency of HANPs was upheld at concentrations ≤ 0.20 mg/mL. Flow cytometric analysis affirms a significant divergence in cell distribution across all cell cycle phases in DPSCs treated with 0.81 mg/mL HANPs. Intriguingly, no variance surfaced in the migratory capacity of DPSCs exposed to HANPs of ≤ 0.40 mg/mL. For osteogenic differentiation, HANPs at concentrations of ≤ 0.40 mg/mL demonstrated the aptitude to incite osteogenic differentiation within DPSCs, facilitating the formation of calcium deposits. In conclusion, combining HANPs and DPSCs shows promise for restoring damaged hard tissues, like bone and teeth, and enhancing regenerative therapies.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"319-330"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered morphology of mucosa-associated lymphoid tissues and epithelium in the nasal cavity and lacrimal apparatus in autoimmune disease-prone MRL/MpJ-Fas^lpr/lpr mice. 自身免疫性疾病易发MRL/MpJ-Faslpr/lpr小鼠鼻腔和泪器粘膜相关淋巴组织和上皮形态的改变

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-25 DOI: 10.1007/s00441-025-03966-2

Masaya Hiraishi, Takashi Namba, Teppei Nakamura, Md Zahir Uddin Rubel, Yasuhiro Kon, Osamu Ichii

{"title":"Altered morphology of mucosa-associated lymphoid tissues and epithelium in the nasal cavity and lacrimal apparatus in autoimmune disease-prone MRL/MpJ-Faslpr/lpr mice.","authors":"Masaya Hiraishi, Takashi Namba, Teppei Nakamura, Md Zahir Uddin Rubel, Yasuhiro Kon, Osamu Ichii","doi":"10.1007/s00441-025-03966-2","DOIUrl":"10.1007/s00441-025-03966-2","url":null,"abstract":"The mucosal epithelium and the mucosa-associated lymphoid tissues (MALTs) protect the mucosa, such as eye and nose, from constant exposure to foreign antigens. As autoimmune disorders can target both epithelium and MALTs, we morphologically investigated the head of MRL/MpJ-Faslpr/lpr, an autoimmune disease-prone mouse, to discuss the pathological crosstalk among autoimmune disorders, mucosal epithelium, and MALTs. Compared to healthy control MRL/MpJ mice, MRL/MpJ-Faslpr/lpr mice had more lymphoid tissues that were diffusely localized beneath the mucosa epithelium in their lacrimal tracts and nasal cavity. Particularly, lacrimal duct- and nasopharynx-associated lymphoid tissues (LDALT, NALT) were identified as the major MALTs in the mouse head. In the nasal mucosa, MRL/MpJ-Faslpr/lpr mice exhibited larger NALT, more frequent non-ciliated epithelial cells, and more goblet cells than in MRL/MpJ mice. NALT in MRL/MpJ-Faslpr/lpr mice contained more proliferating cells than in MRL/MpJ mice. Moreover, LDALT in some MRL/MpJ-Faslpr/lpr mice developed by being directly connected to the bone marrow surrounding the nasolacrimal duct. These data suggested systemic autoimmune disorders could alter the mucosal immunity of the head by affecting both mucosal epithelium and MALTs. These findings are crucial for understanding the pathology of the head mucosal immunity.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"331-345"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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