Cell and Tissue Research最新文献

Functional spatial organization of antennal hair plate sensilla and their development in crickets. 蟋蟀触角毛板感受器的功能空间组织及其发育。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-05-08 DOI: 10.1007/s00441-026-04073-6

Hui Lyu, Hiroto Ogawa

{"title":"Functional spatial organization of antennal hair plate sensilla and their development in crickets.","authors":"Hui Lyu, Hiroto Ogawa","doi":"10.1007/s00441-026-04073-6","DOIUrl":"https://doi.org/10.1007/s00441-026-04073-6","url":null,"abstract":"Antennae of insects are essential mechanosensory organs that facilitate active tactile exploration and spatial navigation. Hair plate sensilla at the base of the antenna flagellum provide proprioceptive inputs to detect the antennae's position. In hemimetabolous insects, such as crickets, the first instar immediately after hatching also possesses antennae; however, the developmental dynamics and spatial organization of antennal hair plates remain poorly understood. We present a comprehensive three-dimensional analysis of the antennal hair plates in crickets (Gryllus bimaculatus) across developmental stages, from the first instar to the adult stage. We demonstrated that hair plate sensilla were present from the first instar and maintained a highly stereotyped spatial arrangement throughout development. Three-dimensional quantification revealed that new sensilla added during molting were formed at specific sites within the hair plate clusters that had existed at the previous stage, maintaining the spatial pattern despite the substantial growth of the antenna. Multidimensional analyses indicated that the spatial arrangement of sensilla was consistent across individuals, suggesting that their organization was under genetic control. Anterograde labeling of sensory afferents showed that sensory neurons in the hair plates converged their axons, extended axon collaterals into the ipsilateral region of the antennal mechanosensory and motor center, and ultimately projected to the subesophageal ganglion. There was no apparent difference in the projection sites among the hair plates, suggesting no clear evidence of topographic organization. Our findings highlight the conserved spatial organization of hair plate sensilla in crickets, suggesting a robust proprioceptive system that provides reliable feedback on antennal position throughout development.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The matrisome, methods, analytical evaluation and potential of decellularized porcine ovaries for fertility preservation. 脱细胞猪卵巢的母体、方法、分析评价及保存生育能力的潜力。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-05-05 DOI: 10.1007/s00441-026-04068-3

Cecibel M León-Félix, Rita L Sousa, Peter E J Bols, Ruth Appeltant

{"title":"The matrisome, methods, analytical evaluation and potential of decellularized porcine ovaries for fertility preservation.","authors":"Cecibel M León-Félix, Rita L Sousa, Peter E J Bols, Ruth Appeltant","doi":"10.1007/s00441-026-04068-3","DOIUrl":"https://doi.org/10.1007/s00441-026-04068-3","url":null,"abstract":"The extracellular matrix (ECM) is being investigated as an innovative artificial ovary 3D scaffold for fertility preservation in humans and animals who are unable to utilise traditional reproductive biotechnologies. This review provides an overview of the ovarian matrisome in native and decellularized ECM (dECM) of pigs, mice and humans. A substantial proportion of components identified in porcine ovarian dECM were also reported in native human ECM, particularly collagens and proteoglycans, while comparisons with murine ovarian ECM (native and decellularized) reveal shared components in these groups as well as in glycoproteins. This indicates a considerable degree of shared core ECM proteins across species and positions porcine ECM as a promising matrix for interspecies applications, including those aimed at humans. The ovarian ECM may provide a favourable environment for the development of oocytes, follicles, or cells from different species during follicle culture or in vitro gametogenesis. However, its composition can vary depending on the ovarian region, the age of the organ, and the decellularization protocol applied. Despite these variations, studies using porcine ovarian dECM have demonstrated follicular growth, oestradiol secretion, embryo formation, and live births in murine models, supporting its potential role in ovarian function recovery. Nevertheless, optimisation of decellularization protocols and rigorous analytical evaluation remain necessary to assess process efficiency and ECM preservation, improving the estimation of its functional efficacy in fertility preservation. In conclusion, porcine ovarian dECM represents a promising natural alternative to synthetic support matrices for fertility preservation techniques in patients and animals.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of asprosin and OLFR734 in reproductive tissues of polycystic ovary syndrome mice: insights into metabolic and reproductive dysfunction. asprosin和OLFR734在多囊卵巢综合征小鼠生殖组织中的表达：对代谢和生殖功能障碍的见解

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-04-25 DOI: 10.1007/s00441-026-04070-9

Sana Khan, Saima Syeda, Anju Shrivastava

{"title":"Expression of asprosin and OLFR734 in reproductive tissues of polycystic ovary syndrome mice: insights into metabolic and reproductive dysfunction.","authors":"Sana Khan, Saima Syeda, Anju Shrivastava","doi":"10.1007/s00441-026-04070-9","DOIUrl":"https://doi.org/10.1007/s00441-026-04070-9","url":null,"abstract":"Asprosin, a fasting-induced adipokine, has been reported to exhibit altered circulating levels in women with polycystic ovary syndrome (PCOS); however, its tissue-specific regulation and the role of its receptor, OLFR734, in PCOS remain poorly understood. In this study, we present the first histological evidence of asprosin and OLFR734 expression in reproductive tissues using dehydroepiandrosterone-induced PCOS mouse models maintained on chow or a high-fat diet (HFD). Metabolic profiling revealed distinct phenotypes, with HFD-fed PCOS mice showing pronounced metabolic disturbances, including increased body weight, glucose intolerance, dyslipidemia, and elevated serum asprosin levels, compared with chow-fed PCOS mice. Asprosin was immunodetected in adipose tissue, ovary, oviduct, and uterus, with increased expression in both PCOS groups. In control ovaries, asprosin was restricted to the theca layer of Graafian follicles. In contrast, PCOS ovaries showed distinct asprosin expression patterns in the theca, granulosa cells, and the antrum of cystic follicles. Asprosin-positive cells were also observed in the oviduct and uterus, with distinct uterine localization in PCOS. OLFR734 showed a similar broad tissue distribution to asprosin, with upregulated expression in both PCOS diet groups. Notably, androgen excess and dietary fat exposure did not produce uniform or additive effects on tissue-level asprosin-OLFR734 expression but instead revealed tissue-specific response patterns under different dietary conditions. Collectively, our findings suggest a potential association of the asprosin-OLFR734 axis with reproductive changes in PCOS, linking metabolic and reproductive dysfunction under diet-induced stress. This work provides a histological and comparative framework, highlighting asprosin and OLFR734 as emerging molecular candidates in PCOS that warrant further investigation for their potential roles in disease pathophysiology.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the hidden anatomy: synchrotron micro-tomography of the stomach, midgut, and organs in Penaeus vannamei and the potential route of Enterocytozoon hepatopaenaei (EHP) infection. 揭开隐藏的解剖结构：凡纳滨对虾胃、中肠和器官的同步微断层扫描和肝乙肝肠胞虫（EHP）感染的潜在途径

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-04-24 DOI: 10.1007/s00441-026-04067-4

Thanapong Kruangkum, Phakkhananan Pakawanit, Kornchanok Jaiboon, Piyachat Sanguanrut, Sukanya Saedan, Kallaya Sritunyalucksana, Siripong Thitamadee, Rapeepun Vanichviriyakit

{"title":"Unlocking the hidden anatomy: synchrotron micro-tomography of the stomach, midgut, and organs in Penaeus vannamei and the potential route of Enterocytozoon hepatopaenaei (EHP) infection.","authors":"Thanapong Kruangkum, Phakkhananan Pakawanit, Kornchanok Jaiboon, Piyachat Sanguanrut, Sukanya Saedan, Kallaya Sritunyalucksana, Siripong Thitamadee, Rapeepun Vanichviriyakit","doi":"10.1007/s00441-026-04067-4","DOIUrl":"https://doi.org/10.1007/s00441-026-04067-4","url":null,"abstract":"Due to the structural complexity of the crustacean digestive system, this study characterizes the three-dimensional (3D) morphology and histology of the juvenile Penaeus vannamei alimentary tract using synchrotron radiation X-ray tomographic microscopy (SR-XTM) and H&E staining. SR-XTM volumetric imaging effectively reveals the structural interconnections among the pyloric stomach (PS), hepatopancreas (HP), and midgut (MG). Histological analysis further distinguishes the regional processing of fine and coarse chyme, which are segregated by a pair of lateral pyloric valves (LPVs). These valves function as mechanical barriers to prevent mixing of coarse and fine chyme within the anterior MG. Furthermore, this study identifies a secondary communication pathway, a sub-LPV channel, that enables a direct connection between the MG and the HP. In situ hybridization of EHP-specific transcripts identifies significant Enterocytozoon hepatopenaei (EHP) infection in cells lining the primary hepatopancreatic chamber and proximal regions of the secondary and tertiary HP tubules. Given that the gastric sieve restricts pathogen transmission by particle size, our findings suggest the sub-LPV channel serves as a critical alternative route for the oral transmission of EHP spores. This study provides a foundational structural framework for understanding shrimp digestive anatomy and host-pathogen internalization.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13106256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homozygous overexpression of ATP6AP2 in female mice and its impact on running-induced modulation of the adult hippocampus. 雌性小鼠ATP6AP2纯合子过表达及其对跑步诱导的成年海马调节的影响。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-04-23 DOI: 10.1007/s00441-026-04071-8

Katharina Bracke, Friederike Klempin, Ulrich Wenzel, Michael Bader, Jörg Peters, Oliver von Bohlen Und Halbach

{"title":"Homozygous overexpression of ATP6AP2 in female mice and its impact on running-induced modulation of the adult hippocampus.","authors":"Katharina Bracke, Friederike Klempin, Ulrich Wenzel, Michael Bader, Jörg Peters, Oliver von Bohlen Und Halbach","doi":"10.1007/s00441-026-04071-8","DOIUrl":"https://doi.org/10.1007/s00441-026-04071-8","url":null,"abstract":"ATP6AP2 (ATPase H⁺ Transporting Accessory Protein 2) also known as (pro)renin receptor (P)PR is expressed in the central nervous system (CNS). In humans, variants of ATP6AP2 have been linked to neurodegenerative disorders and mental retardation and to changes in the volume of the hippocampus-a limbic brain structure that is capable of adult hippocampal neurogenesis. Cell proliferation in the adult dentate gyrus (DG) can be positively modulated by voluntary exercise. Somewhat surprisingly, we have recently shown that ATP6AP2 overexpression does not show any significant effects on the number of newly generated cells in the DG in transgene-heterozygous mice. Therefore, we now analyzed the effects of voluntary exercise on cell proliferation in the adult hippocampus in female mice overexpressing ATP6AP2 on both alleles. ATP6AP2 mRNA levels in forebrain and hippocampus were increased dose-dependently compared with wild-type mice, with homozygous exhibiting stronger effects than heterozygous mice. Full-length ATP6AP2 protein levels were also increased dose-dependently in forebrain, but the protein fragment representing the soluble prorenin receptor was not. In total hippocampus extracts, no significant differences in ATP6AP2 protein levels were found. However, in the DG, ATP6AP2 immunoreactivity was markedly increased. While the running pattern of mice with access to a running wheel was similar between the groups, cell proliferation, as indicated by BrdU staining, was increased in wild-type mice as expected, but failed to increase in homozygous ATP6AP2 mice. This finding indicates that ATP6AP2 overexpression does not affect basal adult cell proliferation per se but inhibits plastic changes in the rate of cell proliferation. Thus, ATP6AP2 appears to selectively limit exercise-dependent regulation in the hippocampus rather than baseline neurogenic capacity.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Articular cartilage thickness alterations in hind limb of young and aged PAC1 gene-deficient mice. 年轻和老年PAC1基因缺陷小鼠后肢关节软骨厚度的改变。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-04-20 DOI: 10.1007/s00441-026-04066-5

Csaba Fillér, Lili Sarolta Kovács, Kálmán Rácz, Yonatan Segal, Judit Vágó, Anna Tóth, Vince Szegeczki, Adél Jüngling, Péter Gergely, Róza Zákány, Dóra Reglődi, Tamás Juhász

{"title":"Articular cartilage thickness alterations in hind limb of young and aged PAC1 gene-deficient mice.","authors":"Csaba Fillér, Lili Sarolta Kovács, Kálmán Rácz, Yonatan Segal, Judit Vágó, Anna Tóth, Vince Szegeczki, Adél Jüngling, Péter Gergely, Róza Zákány, Dóra Reglődi, Tamás Juhász","doi":"10.1007/s00441-026-04066-5","DOIUrl":"10.1007/s00441-026-04066-5","url":null,"abstract":"Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide which was shown to be released in the hypothalamo-hypophyseal system but subsequently demonstrated in the entire nervous system and nearly all peripheral organs, including skeletal elements. PACAP has an important function in the regulation of chondrogenic differentiation, protecting in vitro chondrogenesis during various stresses and in osteogenesis. PACAP knockout (KO) mice show early signs of aging. Its most potent receptor is PAC1-R, the activation of which leads to enhanced Sox9 expression and subsequently, increase in the expression of collagen type II, glycosaminoglycans, and aggrecan. In the present experiments, we investigated the effect of the absence of PAC1 receptor in PAC1 KO homozygous and heterozygous mice focusing on joints of hind limb in young and aged animals. Thickness and extracellular matrix content of articular cartilage of joints increased in the absence of PAC1 receptor with aging. A thicker cartilage was detected in aged animals in mechanically affected joints. Interestingly, the disturbance of PACAP signaling pathways increased the nuclear translocation of P-Sox9 transcription factor in various joints. In summary, the alteration of PAC1 receptor regulated signalization elevated cartilage formation and protected cartilage architecture during aging suggesting a balancing effect of the receptor in chondrogenesis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13092545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Lmx1a/b allelic series reveals the role of Lmx1 genes in cochlear nuclei development. 一个Lmx1a/b等位基因序列揭示了Lmx1基因在耳蜗核发育中的作用。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-04-17 DOI: 10.1007/s00441-026-04064-7

Igor Y Iskusnykh, Bernd Fritzsch, Ebenezer N Yamoah, Ekaterina Y Steshina, Victor V Chizhikov

{"title":"An Lmx1a/b allelic series reveals the role of Lmx1 genes in cochlear nuclei development.","authors":"Igor Y Iskusnykh, Bernd Fritzsch, Ebenezer N Yamoah, Ekaterina Y Steshina, Victor V Chizhikov","doi":"10.1007/s00441-026-04064-7","DOIUrl":"10.1007/s00441-026-04064-7","url":null,"abstract":"Lmx1a/b paralogous genes, which arose from the invertebrate Lmx1b-like gene, are critical for hearing in multiple vertebrate species, and mutations in these genes cause hearing deficits in humans. While the unique and redundant functions of Lmx1a/b in the inner ear are well established, their contribution to the development of the cochlear nuclei, which process and relay auditory information to the brain, is poorly understood. Since cochlear nuclei maturate postnatally, here we analyzed Lmx1a+/-;Lmx1b+/-, Lmx1a-/-, and Lmx1a-/-;Lmx1b+/- mice that survive past birth. Loss of Lmx1a reduced distinct populations of excitatory neurons in dorsal (DCN) and ventral (VCN) cochlear nuclei and their innervation from the inner ear. Additional loss of one Lmx1b copy made Lmx1a-/- phenotypes more severe, revealing that Lmx1b acts redundantly with Lmx1a. Unlike Lmx1a-/- mice, excitatory neurons were not affected in Lmx1a+/-;Lmx1b+/- mice. Thus, while cochlear nuclei are sensitive to Lmx1a/b gene dosage, these genes are not completely equivalent, and Lmx1a has a more profound role in cochlear nuclei development. Lmx1a-/- and especially Lmx1a-/-;Lmx1b+/- embryos had fewer Atoh1+ progenitors that produce excitatory neurons of the cochlear nuclei, and reduced Bmp6 expression in the roof plate, the signaling center that induces these progenitors via Bmp signaling. We found that Lmx1a is the primary regulator of Bmp6, whereas Lmx1b contributes only in the absence of Lmx1a. Thus, Lmx1a plays a major role in the formation of the mature structure and connectivity of both the DCV and VCN, and Lmx1b acts redundantly to Lmx1a but only partially compensates for Lmx1a loss.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13086785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucose transporter 1 deficiency impairs glucose homeostasis, cell proliferation, and morphology in human embryonic kidney cells 293. 葡萄糖转运蛋白1缺乏损害人胚胎肾细胞的葡萄糖稳态、细胞增殖和形态293。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-03-25 DOI: 10.1007/s00441-026-04061-w

Yash Mehta, Abraham Jacob Al-Ahmad

{"title":"Glucose transporter 1 deficiency impairs glucose homeostasis, cell proliferation, and morphology in human embryonic kidney cells 293.","authors":"Yash Mehta, Abraham Jacob Al-Ahmad","doi":"10.1007/s00441-026-04061-w","DOIUrl":"10.1007/s00441-026-04061-w","url":null,"abstract":"Glucose represents a major source of energy for mammalian cells. In such cells, glucose uptake is facilitated by the presence of various glucose transporters (GLUTs). Amongst the different GLUT isoforms expressed in mammalian cells, GLUT1 is a major isoform expressed during development, but becomes restricted to a select number of cell types in adult cells, which include red blood cells, brain microvascular endothelial cells, or astrocytes. GLUT1 deficiency syndrome (GLUT1DS) is an autosomal dominant neurological disease characterized by reduced cerebral glucose and lactate uptake in patients. We previously documented the impact of GLUT1DS on the glucose uptake and homeostasis in human pluripotent stem cell-derived brain microvascular endothelial cells and astrocytes. Although such cells showed similarities in terms of impaired glucose uptake, we also noticed differences in their metabolic adaptation to such impairment. This study aims to assess the impact of GLUT1DS on non-cerebral cells by investigating the impact of impaired GLUT1 in GLUT1-deficient human embryonic kidney cells (GLUT1D-HEK293). Our results suggest that GLUT1D-HEK293 cells were viable but displayed altered cell doubling and cell morphology, reduced glucose uptake and consumption (with no apparent compensation by other GLUT isoforms), while accompanied by a severe reduction in cell glycolytic activity and a marked deficit in ATP production. Taken together, our study demonstrates that the impairment of GLUT1 activity in human cells shares common phenotypic outcomes between various cell types but also displays unique cellular responses when it comes to metabolic adaptation to energy deficit, partially explaining the impact on tissues in GLUT1DS patients.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative and immunohistochemical analyses on hepatic zonation in vertebrates with a special reference to evolution. 脊椎动物肝脏分区的比较和免疫组织化学分析，特别涉及到进化。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-03-25 DOI: 10.1007/s00441-026-04063-8

Nobuyoshi Shiojiri, Noriaki Ota, Haruka Hirose, Tatsuya Fukuda, Hideaki Kato, Hikari Maeda, Shuji Takabayashi, Takahiko Kawasaki, Kazuho Ikeo

{"title":"Comparative and immunohistochemical analyses on hepatic zonation in vertebrates with a special reference to evolution.","authors":"Nobuyoshi Shiojiri, Noriaki Ota, Haruka Hirose, Tatsuya Fukuda, Hideaki Kato, Hikari Maeda, Shuji Takabayashi, Takahiko Kawasaki, Kazuho Ikeo","doi":"10.1007/s00441-026-04063-8","DOIUrl":"10.1007/s00441-026-04063-8","url":null,"abstract":"The mammalian liver lobules have zonation for various hepatic functions. It remains to be precisely determined which vertebrate taxa exhibit the hepatic zonation. There are two types of liver architectures in vertebrates: the portal triad type (roughly vertebrates except teleosts) and the non-portal triad type (teleosts). The aim of the present study is to determine histochemically and immunohistochemically how the hepatic zonation is established in vertebrates with a reference to the liver architectures and evolution. As a result, the typical hepatic zonation of metabolic enzymes, such as phosphoenolpyruvate carboxykinase and Cyp1a2, was detectable in mammals, including the opossums, pigs, dolphins, guinea pigs, mice, and marmosets. They also consistently exhibited pericentral expression of glutamine synthase, one of ammonia-metabolizing enzymes, which was undetectable in other vertebrates. Murine livers had a more remarkable zonation of cell adhesion molecules and carbamoyl-phosphate synthase I, which other mammals did not exhibit. Chicken and Japanese quail livers showed zonation of alkaline phosphatase (ALP) activity in sinusoidal cells, but no zonation of metabolic enzymes. The amphibian and reptilian livers showed poor zonation. Although many actinopterygians did not exhibit hepatic zonation, some teleost livers showed zonation for the accumulation of periodic acid-Schiff-positive substances and sinusoidal ALP activity. Grass puffer exhibited zonation of transcription factors and cell adhesion molecules. These data indicated that hepatic zonation was not associated with the two types of liver architectures. Mammals and some teleosts may have acquired remarkable hepatic zonation during evolution. Zonation of pericentral glutamine synthase expression is unique to mammalian species among vertebrates.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13018004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early intestinal barrier changes in A53T transgenic Parkinson's disease mice. A53T转基因帕金森病小鼠早期肠道屏障的改变

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2026-03-24 DOI: 10.1007/s00441-026-04062-9

Myat Noe Han, Olivia Artaiz, Matthew C Rowe, David I Finkelstein, Shanti Diwakarla, Rachel M McQuade

{"title":"Early intestinal barrier changes in A53T transgenic Parkinson's disease mice.","authors":"Myat Noe Han, Olivia Artaiz, Matthew C Rowe, David I Finkelstein, Shanti Diwakarla, Rachel M McQuade","doi":"10.1007/s00441-026-04062-9","DOIUrl":"10.1007/s00441-026-04062-9","url":null,"abstract":"Gut dysfunction commonly precedes motor symptoms in Parkinson's disease (PD), but the mechanistic sequence of gut versus brain pathology remains unclear. This work aimed to define the timing of intestinal barrier dysfunction relative to central nervous system (CNS) changes in the A53T α-synuclein transgenic mouse model of PD. Functional and molecular assessments of the gastrointestinal tract (ileum and colon) were conducted at 12 and 36 weeks. We measured in vivo and ex vivo intestinal permeability, nutrient absorption, histomorphology, goblet cell density, and expression of MUC2 and Claudin-1. Inflammatory markers (CRP, TNF-α, CD45) were quantified in plasma and gut tissues. A53T mice exhibited increased intestinal permeability at 12 and 36 weeks, with transiently elevated ex vivo transepithelial electrical resistance (TER) at 12 weeks. Nutrient absorption remained intact. Morphological changes included widened villi and crypts, altered mucin expression, and early reductions in Claudin-1 in the ileum and the colon while inflammatory markers remained largely unchanged. These findings suggest that gut dysfunction precedes known central pathology in A53T mice, supporting further investigation into the gut as an early site of pathology and a potential therapeutic target in PD.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"404 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13009022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147503344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0