Cell and Tissue Research最新文献

ZO-2 is a scaffold at the centriole and mitotic spindle poles that enhances microtubule stability and supports the proper development of mitotic spindles and cilia. ZO-2是中心粒和有丝分裂纺锤体两极的支架，增强微管稳定性，支持有丝分裂纺锤体和纤毛的正常发育。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-07-29 DOI: 10.1007/s00441-025-03992-0

Sara Vega-Torreblanca, Diana Cristina Pinto-Dueñas, Christian Hernández-Guzmán, Dolores Martín-Tapia, Lourdes Alarcón, Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Sirenia González-Pozos, Josué David Hernández-Varela, Leticia Ramírez-Martínez, Esther López-Bayghen, José Jorge Chanona-Pérez, Lorenza González-Mariscal

{"title":"ZO-2 is a scaffold at the centriole and mitotic spindle poles that enhances microtubule stability and supports the proper development of mitotic spindles and cilia.","authors":"Sara Vega-Torreblanca, Diana Cristina Pinto-Dueñas, Christian Hernández-Guzmán, Dolores Martín-Tapia, Lourdes Alarcón, Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Sirenia González-Pozos, Josué David Hernández-Varela, Leticia Ramírez-Martínez, Esther López-Bayghen, José Jorge Chanona-Pérez, Lorenza González-Mariscal","doi":"10.1007/s00441-025-03992-0","DOIUrl":"https://doi.org/10.1007/s00441-025-03992-0","url":null,"abstract":"Previous studies revealed the presence of several tight junction (TJ) proteins in the centrosome and their interaction with various centriolar proteins, prompting us to analyze whether this also applies to the TJ protein ZO-2. Here, we found that ZO-2 colocalizes with CEP164 in the distal appendage of the mother centriole and is also present in the pericentriolar region, mitotic spindle poles, the basal body of primary cilia, and the tail of spermatozoa. The absence of ZO-2 altered the cellular content of centriolar proteins CEP164, centriolin, and CEP135, but did not change the morphology of centrioles. ZO-2 depletion inhibits the development of astral and mitotic spindle microtubules expressing EB1. At the spindle poles, ZO-2 depletion increases the accumulation of NuMA while reducing the levels of kinesin KIF14 and the TPX2 scaffold, and the accumulation of the kinase p-Aurora, leading to a decrease in mitotic spindle length, microtubule instability, and abnormal chromosome congression. KIF14, NuMA, and p-Aurora co-immunoprecipitate with ZO-2, and NuMA and Aurora-A bind to different segments of ZO-2. At the ciliary basal body, ZO-2 depletion reduces the content of CEP164, KIF14, and IFT-B protein IFT57, while increasing the expression of p-Aurora and pAKT. These changes block primary cilium development and the response to Sonic Hedgehog signaling pathway stimulation. These results suggest that, rather than being a centrosomal architectural component, ZO-2 enhances microtubule stability and serves as a scaffold that facilitates the adequate accumulation of spindle pole and centriole proteins, allowing proper poleward spindle microtubule flux and cilia development.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional role of circadian clock system in steroidogenesis and cell death pathways during corpus luteum regression in cattle. 生理时钟系统在黄体退化过程中甾体生成和细胞死亡途径中的功能作用。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-24 DOI: 10.1007/s00441-025-03997-9

Jing Zhang, Hanako Bai, Manabu Kawahara, Ahmed Z Balboula, Masashi Takahashi

{"title":"Functional role of circadian clock system in steroidogenesis and cell death pathways during corpus luteum regression in cattle.","authors":"Jing Zhang, Hanako Bai, Manabu Kawahara, Ahmed Z Balboula, Masashi Takahashi","doi":"10.1007/s00441-025-03997-9","DOIUrl":"https://doi.org/10.1007/s00441-025-03997-9","url":null,"abstract":"The corpus luteum (CL) is an ovarian structure that secretes progesterone (P4) following ovulation, playing a crucial role in regulating the estrous cycle and maintaining pregnancy. Luteolysis, the structural and functional degradation of the CL, occurs through apoptosis and autophagy. Recent studies suggest that the circadian clock (CC) system, particularly the gene NR1D1, is involved in these processes. This study investigated the role of NR1D1 in bovine CL regression using an ex vivo model treated with prostaglandin F2α (PGF2α), the NR1D1 agonist GSK4112, and the antagonist SR8278. CL samples were classified into four estrous cycle stages based on ovarian morphology and analyzed for P4 secretion, as well as gene and protein expression related to steroid synthesis, the CC system, autophagy, and apoptosis. P4 levels, steroid synthesis-related genes, and CC system-related genes, including NR1D1, were highly expressed in the mid and late stages of the CL, whereas autophagy- and apoptosis-related genes peaked during regression. Western blotting and immunofluorescence revealed increased expression of NR1D1 and BMAL1 in the mid and late stages, while LC3 and CTSB were most prominent during regression. PGF2α treatment reduced NR1D1 and BMAL1 expression, along with decreased P4 levels and increased apoptosis markers. GSK4112 suppressed steroid synthesis while upregulating autophagy- and apoptosis-related genes. Conversely, SR8278 reversed PGF2α-induced luteal regression, restoring P4 and steroidogenic gene expression while suppressing CTSB. These findings suggest that NR1D1 interacts with PGF2α to regulate CL regression, highlighting the CC system as a potential target for improving reproductive efficiency in cattle.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanobody-based Pannexin1 channel inhibitors increase survival after cardiac ischemia/reperfusion. 基于纳米体的Pannexin1通道抑制剂增加心脏缺血/再灌注后的生存。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-23 DOI: 10.1007/s00441-025-03994-y

Olga M Rusiecka, Filippo Molica, Linda Clochard, Raf Van Campenhout, Timo W M De Groof, Viviane Bes, Nick Devoogdt, Serge Muyldermans, Mathieu Vinken, Brenda R Kwak

{"title":"Nanobody-based Pannexin1 channel inhibitors increase survival after cardiac ischemia/reperfusion.","authors":"Olga M Rusiecka, Filippo Molica, Linda Clochard, Raf Van Campenhout, Timo W M De Groof, Viviane Bes, Nick Devoogdt, Serge Muyldermans, Mathieu Vinken, Brenda R Kwak","doi":"10.1007/s00441-025-03994-y","DOIUrl":"https://doi.org/10.1007/s00441-025-03994-y","url":null,"abstract":"Reperfusion following myocardial infarction salvages the ischemic heart but paradoxically exacerbates injury. Yet, efficient treatment for cardiac ischemia/reperfusion injury is still missing in clinics. ATP release through Pannexin1 (PANX1) channels facilitates recruitment of leukocytes to the injured myocardium. Thus, PANX1 channel inhibition might confer cardioprotection. Currently available PANX1 channel blockers lack specificity or in vivo stability. Nanobodies offer a new therapeutic modality given their high target affinity, small size, and deep tissue penetration. Nanobodies targeting Panx1 were recently introduced. Here, their target specificity and selective PANX1 channel inhibition for cardiovascular purposes were validated in vitro. The two most promising candidates were further examined in the context of cardiac ischemia/reperfusion injury. Nanobody-1 (Nb1) and Nb9 reduced neutrophil adhesion to an endothelial monolayer. Nb1 did not affect left ventricular function ex vivo; however, Nb9 tended to diminish the performance of isolated hearts. Finally, in vivo application of Nb1, but not of Nb9 or a control Nb, at the onset of reperfusion increased the survival rate of mice. However, the infarct size observed after treatment with Nb1 was similar than the one found after treatment with the control Nb. In conclusion, Nb1 efficiently and specifically inhibits ATP release from endothelial cells thereby limiting leukocyte adhesion and improving the outcome of cardiac ischemia/reperfusion in mice. This warrants further studies to unveil the detailed molecular mechanism underlying the beneficial effects of Nb1.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Aqp1 expression by osmotic balance in fenestrated endothelial cells of the posterior lobe of the pituitary. 垂体后叶开孔内皮细胞渗透平衡对Aqp1表达的调节。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-22 DOI: 10.1007/s00441-025-03995-x

Takashi Nakakura, Takeshi Suzuki

{"title":"Regulation of Aqp1 expression by osmotic balance in fenestrated endothelial cells of the posterior lobe of the pituitary.","authors":"Takashi Nakakura, Takeshi Suzuki","doi":"10.1007/s00441-025-03995-x","DOIUrl":"https://doi.org/10.1007/s00441-025-03995-x","url":null,"abstract":"The posterior lobe (PL) of the vertebrate pituitary is richly vascularized with a dense network of fenestrated capillaries. In this study, we found that the expression of Aqp1, which encodes a plasma membrane-localized water channel protein, was significantly higher in endothelial fractions isolated from the rat PL than in those isolated from the anterior lobe (AL). Immunohistochemistry revealed aquaporin 1 (AQP1)-positive signals in fenestrated endothelial cells of the PL. Furthermore, immunoelectron microscopy demonstrated the presence of AQP1 signals on both the luminal and abluminal plasma membranes of these cells. AQP1 plays a pivotal role in facilitating water movement across the plasma membrane in response to changes in osmotic pressure on a cell. To investigate the effect of hyperosmolarity, we examined the expression levels of Aqp1 in the PL of water-deprived rats as well as in isolated endothelial cells of the PL cultured in a hyperosmotic medium supplemented with raffinose. Immunohistochemical analysis showed no changes in the proportion of AQP1-positive endothelial cells or in subcellular localization of AQP1 in cultured endothelial cells of the PL under hyperosmotic conditions. In contrast, analysis using quantitative real-time PCR revealed that hyperosmolar conditions significantly downregulated Aqp1 expression in cultured endothelial cells. These findings suggest that Aqp1expression in fenestrated capillaries in the PL is regulated by osmotic pressure of the interstitial fluid. Our results indicate that AQP1 is selectively expressed in fenestrated capillaries of the PL and plays a crucial role in maintaining water homeostasis in this region.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical relationships of huntingtin-associated protein 1 with choline acetyltransferase in the forebrain cholinergic nuclei of adult mice. 亨廷顿蛋白相关蛋白1与成年小鼠前脑胆碱能核胆碱乙酰转移酶的免疫组化关系。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-19 DOI: 10.1007/s00441-025-03996-w

Mirza Mienur Meher, Md Nabiul Islam, Akie Yanai, Marya Afrin, Mir Rubayet Jahan, Kanako Nozaki, Koh-Hei Masumoto, Koh Shinoda

{"title":"Immunohistochemical relationships of huntingtin-associated protein 1 with choline acetyltransferase in the forebrain cholinergic nuclei of adult mice.","authors":"Mirza Mienur Meher, Md Nabiul Islam, Akie Yanai, Marya Afrin, Mir Rubayet Jahan, Kanako Nozaki, Koh-Hei Masumoto, Koh Shinoda","doi":"10.1007/s00441-025-03996-w","DOIUrl":"https://doi.org/10.1007/s00441-025-03996-w","url":null,"abstract":"Huntingtin-associated protein 1 (HAP1) is a core component of the stigmoid body (STB) and a neuroprotective interactor with the causative agents of several neurodegenerative diseases (NDs). The cholinergic system is often affected by NDs. Our previous studies suggest that cholinergic brainstem/spinal cord motoneurons are more vulnerable to neurodegeneration due to a lack of STB/HAP1 protectivity. The forebrain cholinergic nuclei are also major neurodegenerative/psychotic targets; however, the relationships of HAP1 with choline acetyltransferase (ChAT) have yet to be determined there. This study used western blotting and immunohistochemistry to evaluate the comparative distribution of HAP1 with ChAT and their immunohistochemical relationships in the adult mouse forebrain cholinergic nuclei. The results showed that HAP1-immunoreactive (ir) neurons were highly distributed in the basal forebrain cholinergic nuclei, including medial septal nucleus (MS), nucleus of vertical limb of the diagonal band of Broca (VDB), nucleus of horizontal limb of the diagonal band of Broca (HDB), and substantia innominata basal part (SIB). HAP1-ir neurons were sporadically scattered in the striatum. The significantly highest co-expression ratio of HAP1 with ChAT was observed in MS and VDB. In contrast, the ChAT-ir neurons never contained HAP1 in the caudate putamen of the striatum. These suggest that, due to having putative HAP1 protectivity, the cholinergic neurons in MS and VDB that are mainly projected to the hippocampal/parahippocampal regions might be protected to regulate social memory formation, emotion, and other psychological functions. Consequently, the lack of HAP1 protectivity might make cholinergic neurons in the striatum more prone to neurodegeneration in certain NDs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes. 更正：β -阻滞蛋白1参与小鼠软骨细胞中透明质酸降解刺激的分解代谢反应。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-18 DOI: 10.1007/s00441-025-03990-2

引用次数: 0

Male reproductive system in Aegla (Decapoda: Aeglidae) and its anatomic-histological and ultrastructural relationship with other anomuran crabs. 海蟹（十足目：海蟹科）雄性生殖系统及其与其他异形蟹的解剖组织学和超微结构关系。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-18 DOI: 10.1007/s00441-025-03993-z

Bárbara Paiva, Caio Santos Nogueira, Gustavo Monteiro Teixeira, Fernando José Zara

{"title":"Male reproductive system in Aegla (Decapoda: Aeglidae) and its anatomic-histological and ultrastructural relationship with other anomuran crabs.","authors":"Bárbara Paiva, Caio Santos Nogueira, Gustavo Monteiro Teixeira, Fernando José Zara","doi":"10.1007/s00441-025-03993-z","DOIUrl":"https://doi.org/10.1007/s00441-025-03993-z","url":null,"abstract":"The male reproductive system (MRS) of decapods in the genus Aegla remains poorly understood from both histological and ultrastructural perspectives. This study provides a comparative description of the anatomy, histology, and ultrastructure of the MRS in multiple Aegla species, with the aim of exploring their phylogenetic relationships with representatives of the superfamilies Lomisoidea and Chirostyloidea. Anatomically, the MRS of Aegla is located in the cephalothorax and consists of a bilateral structure. The testes are connected by a central commissure and independently open into each vas deferens. The vas deferens is a translucent tube subdivided into proximal, medial, and distal regions. Across all regions, the seminal fluid contains few free spermatozoa, and spermatophores are absent. This fluid comprises two types of secretion: type I (basophilic) and type II (strongly basophilic), both composed mainly of proteins and acidic polysaccharides, with interspecific variation in acidic polysaccharide content. Aegla spermatozoa exhibit a standard organization, organized into two hemispheres-cytoplasmic and nuclear-and possess an acrosome vesicle with two concentric layers. Comparatively, their ultrastructure closely resembles that of Lomis hirta, whereas members of Chirostyloidea lack similar features, indicating an evolutionary divergence. These findings contribute important insights into the evolutionary history of Anomura, highlighting the absence of spermatophores in Aegla-a condition typical of this infraorder-and underscoring the similarity in spermatozoa ultrastructure between Aegla and Lomis, likely reflecting a shared ancestral trait.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Guanabenz mitigates the neuropathological alterations and cell death in Alzheimer's disease. 备注：瓜纳苯可减轻阿尔茨海默病的神经病理改变和细胞死亡。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-16 DOI: 10.1007/s00441-025-03998-8

Abhishek Singh, Parul Gupta, Shubhangini Tiwari, Amit Mishra, Sarika Singh

引用次数: 0

Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue. 脂肪组织去泛素化酶CYLD失活小鼠的表型分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-02 DOI: 10.1007/s00441-025-03989-9

Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos

{"title":"Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue.","authors":"Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos","doi":"10.1007/s00441-025-03989-9","DOIUrl":"https://doi.org/10.1007/s00441-025-03989-9","url":null,"abstract":"The cylindromatosis tumor suppressor (CYLD) is a deubiquitinating enzyme that has been implicated in lipid metabolism. More specifically, CYLD has been associated with lipid homeostasis in Drosophila melanogaster, and CYLD deficiency in mammals has been linked to dysregulation of lipid metabolism in the liver. Comprehensive tissue RNA expression analyses have revealed comparable levels of Cyld mRNA expression in the adipose tissue and liver, the organs that, together with skeletal muscle, primarily regulate lipid homeostasis. In the present study, the role of CYLD in mammalian adipose tissue homeostasis and function was investigated, utilizing a relevant conditional mouse model of CYLD inactivation that permits tissue-specific elimination of the catalytic domain of CYLD. Mutant mice displayed reduced weight-gain rate compared to controls when fed a normal or high-fat diet. Histological analysis of crown-like structures (CLS) indicated a reduced inflammatory response in the white adipose tissue of mutants. Our data collectively demonstrate that CYLD plays a pivotal role in regulating key metabolic parameters and modulating inflammatory responses within adipose tissue.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection. COVID-19急性感染患者呼吸肌组织病理学分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-05-05 DOI: 10.1007/s00441-025-03973-3

Laura Steingruber, Simona Handtke, Franziska Schweiger, Stefan Schiele, Bruno Märkl, Marco Koch

{"title":"Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection.","authors":"Laura Steingruber, Simona Handtke, Franziska Schweiger, Stefan Schiele, Bruno Märkl, Marco Koch","doi":"10.1007/s00441-025-03973-3","DOIUrl":"10.1007/s00441-025-03973-3","url":null,"abstract":"Coronavirus-disease 2019 (COVID-19) affects the respiratory system with high morbidity in elderly and comorbid patients. Acute COVID-19 infection (CI) primarily leads to respiratory failure, long-term effects on respiratory skeletal muscle however remain vague. Thus, histopathological marker expression of oxidative stress, inflammation, satellite cell activity, myosin fiber composition, and cellular senescence were analyzed in intercostal muscle and diaphragm to compare respiratory muscle degeneration (RMD) in deceased CI-positive and control patients. Beside CI, the impact of BMI, age, sex, ventilation status, and duration of hospitalization on RMD were evaluated. CI-positive patients exhibited higher numbers of regenerative stem cells, but no association between CI status and RMD was observed. Interestingly, ventilation support and lung-associated comorbidities had no effect on expression of RMD markers (p > 0.05). However, intercostal muscle showed BMI-dependent changes in expression of RMD markers, regardless of the CI status, with increased cytokine expression (p = 0.04), reduced antioxidative capacity (p = 0.05), and low stem cell prevalence (p = 0.02) in patients with high BMI. Moreover, elderly patients demonstrated increased oxidative stress (p = 0.001) and cell senescence (p = 0.03) independent of CI status. Notably, immobility drives muscle fiber transformation to Myosin ST (p = 0.03), since prolonged hospitalization correlated with muscle fiber type shift. Limitations included incomplete retrospective data collection and absence of adequate samples for molecular analyses. Together, RMD is influenced by BMI, age and immobility rather than the CI status alone. Future studies including larger cohorts, molecular analyses, and evaluation of patient data in addition to CI status alone, will further support meaningful analyses and interpretation of RMD and its impact on post CI recovery.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"59-68"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0