Cell and Tissue Research最新文献_第2页

CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics. 大鼠垂体中间叶的CD9/ sox2阳性细胞表现出间充质干细胞的特征。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-14 DOI: 10.1007/s00441-024-03947-x

Ayano Shindo, Morio Azuma, Ken Fujiwara, Saishu Yoshida, Kotaro Horiguchi

{"title":"CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics.","authors":"Ayano Shindo, Morio Azuma, Ken Fujiwara, Saishu Yoshida, Kotaro Horiguchi","doi":"10.1007/s00441-024-03947-x","DOIUrl":"https://doi.org/10.1007/s00441-024-03947-x","url":null,"abstract":"Adult tissue stem cells of the anterior pituitary gland, CD9/SOX2-positive cells, are believed to exist in the marginal cell layer (MCL) bordering the residual lumen of the Rathke's pouch. These cells migrate from the intermediate lobe side of the MCL (IL-MCL) to the anterior lobe side of the MCL and may be involved in supplying hormone-producing cells. Previous studies reported that some SOX2-positive cells of the anterior lobe differentiate into skeletal muscle cells. These findings suggest that CD9/SOX2-positive cells in the anterior pituitary have mesenchymal stem cell (MSC) properties. To substantiate this hypothesis, we examined whether CD9-positive cells isolated from IL-MCL of adult male rats differentiate into mesenchymal cells, such as endothelial cells, adipocytes, chondrocytes, and osteocytes. Immunohistochemical analysis revealed that the CD9-positive cells were positive for the MSC markers, CD349, CD105, CD271, and CD273 and were detected in the early postnatal period at the boundary between the posterior and intermediate lobes but not in the embryonic period. In addition, some adult tissue stem cells derived from neural crest cells and bone marrow haematopoietic stem cells were positive for both CD9 and MSC markers, indicating that several CD9/SOX2-positive cells in the IL-MCL of the pituitary gland are MSCs that invaded from external tissues during pituitary development in the early postnatal period and exist in the adult tissue stem cells as suppliers of hormone-producing and endothelial cells in the anterior lobe. These findings should have implications for the application of CD9/SOX2-positive cells in regenerative therapy of the pituitary.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and histochemical characteristics of the foregut, midgut, and hindgut, and their alterations during ovarian development in female freshwater prawn, Macrobrachium rosenbergii. 罗氏沼虾雌虾前、中、后肠形态和组织化学特征及其在卵巢发育过程中的变化。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-13 DOI: 10.1007/s00441-024-03948-w

Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul

{"title":"Morphological and histochemical characteristics of the foregut, midgut, and hindgut, and their alterations during ovarian development in female freshwater prawn, Macrobrachium rosenbergii.","authors":"Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul","doi":"10.1007/s00441-024-03948-w","DOIUrl":"https://doi.org/10.1007/s00441-024-03948-w","url":null,"abstract":"The anatomical, histological, and histochemical characteristics of the foregut (FG), midgut (MG), and hindgut (HG), as well as their alterations during the ovarian cycle in female prawns, Macrobrachium rosenbergii, were investigated. The esophagus (ESO), cardia (CD), and pylorus (PY) are the main components of the FG. An epithelium (Ep) with thick cuticle (Cu) layers lining the ESO, and the ESO is encircled by the ESO glands. The CD has a thick musculature, whereas the Ep of the PY are characterized by numerous villi and columnar Ep cells with a thinner layer of Cu. The inner longitudinal (LM) and the outer circular (CM) muscles were both present in the PY. The MG is lined by Ep cells which are connected to the basement membrane, and it lacks Cu. Microvilli, and subapical vacuoles are visible on the apical surface of Ep cells of the MG. The outermost layer is characterized by a dense strip of elastic fibers and a cluster of collagen fibers. The HG has the Ep cells with a thin Cu layer, and the HG glands form a rosette-like structure. The HG is surrounded by the CM and the LM fibers. The reactivities of Periodic Acid Schiff and Alcian Blue in these digestive organs altered throughout the ovarian cycle, and this was supported by the increased expression of mucin levels as ovarian maturation progressed. Our results offer novel and significant insights into the anatomical and histochemical structures of these digestive organs, and demonstrate a significant correlation between ovarian development and feeding in the female prawn, M. rosenbergii.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurological confirmation of periplanone-D exploitation as a primary sex pheromone and counteractions of other components in the smoky brown cockroach Periplaneta fuliginosa. 烟熏棕色蟑螂大蠊d作为主要性信息素的神经学证实及其他成分的拮抗作用。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-10 DOI: 10.1007/s00441-024-03935-1

Mana Domae, Masazumi Iwasaki, Hiroshi Nishino

{"title":"Neurological confirmation of periplanone-D exploitation as a primary sex pheromone and counteractions of other components in the smoky brown cockroach Periplaneta fuliginosa.","authors":"Mana Domae, Masazumi Iwasaki, Hiroshi Nishino","doi":"10.1007/s00441-024-03935-1","DOIUrl":"10.1007/s00441-024-03935-1","url":null,"abstract":"The smoky brown cockroach, Periplaneta fuliginosa, is a peridomestic pest inhabiting broad regions of the world from temperate to subtropical zones. In common with other related species such as the American cockroach, Periplaneta americana, female-emitted sex pheromone components, named periplanones, are known to be key volatiles that elicit long-range attraction and courtship rituals in males. How periplanones are processed in the nervous system has been entirely unexplored in P. fuliginosa. By using pheromone compounds, periplanones A, B, C, and D, as stimulants to the antenna, we identified four distinct types of interneurons (projection neurons) that relay pheromonal signals from a single olfactory glomerulus of the first-order olfactory center (antennal lobe) to higher-order centers in the ipsilateral hemibrain. All glomeruli innervated by pheromone-responsive projection neurons clustered near the antennal nerve entrance of the antennal lobe. The projection neuron with dendrites in the largest glomerulus was tuned specifically to periplanone-D, and adding other components to periplanone-D counteracted the excitation elicited by periplanone-D alone. Likewise, the projection neuron with dendrites in the second largest glomerulus and that with dendrites in a medium-sized glomerulus were tuned to periplanone-A and periplanone-B, respectively. Our results are, therefore, consistent with behavioral findings that periplanone-D alone acts as a primary sex attractant and that other components act as potential behavioral antagonists. Moreover, a comparison of the glomeruli in P. fuliginosa and P. americana suggested that there are differences in the sizes of homologous glomeruli, as well as in the ligands they process.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform. 使用Xenium平台对人类骨骼肌进行原位空间转录组学分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-09 DOI: 10.1007/s00441-024-03945-z

Nejc Umek, Marija Meznarič, Žiga Šink, Kaja Blagotinšek Cokan, Uršula Prosenc Zmrzljak, Simon Horvat

{"title":"In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform.","authors":"Nejc Umek, Marija Meznarič, Žiga Šink, Kaja Blagotinšek Cokan, Uršula Prosenc Zmrzljak, Simon Horvat","doi":"10.1007/s00441-024-03945-z","DOIUrl":"https://doi.org/10.1007/s00441-024-03945-z","url":null,"abstract":"Traditional transcriptomic studies often overlook the complex heterogeneity of skeletal muscle, as they typically isolate RNA from mixed muscle fibre and cell populations, resulting in an averaged transcriptomic profile that obscures fibre type-specific differences. This study assessed the potential of the recently developed Xenium platform for high-resolution spatial transcriptomic analysis of human skeletal muscle histological sections. Human vastus lateralis muscle samples from two individuals were analysed using the Xenium platform and Human Multi-Tissue and Cancer Panel targeting 377 genes complemented by staining of successive sections for Myosin Heavy Chain isoforms to differentiate between type 1 and type 2 muscle fibres. Manual segmentation of muscle fibres allowed accurate comparisons of transcript densities across fibre types and subcellular regions, overcoming limitations in the platform's automated segmentation. The analysis revealed higher transcript density in type 1 fibres, particularly in nuclear and perinuclear areas, and identified 191 out of 377 genes with differential expression between muscle fibres and perimysium. Genes such as PROX1, S100A1, LGR5, ACTA2, and LPL exhibited higher expression in type 1 fibres, whereas PEBP4, CAVIN1, GATM, and PVALB in type 2 fibres. We demonstrated that the Xenium platform is capable of high-resolution spatial in situ transcriptomic analysis of skeletal muscle histological sections. This study demonstrates that, with manual segmentation, the Xenium platform effectively performs fibre type-specific transcriptomic analysis, providing new insights into skeletal muscle biology.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis. 淡水小龙虾造血组织的组织与表达分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-04 DOI: 10.1007/s00441-024-03943-1

Thanapong Kruangkum, Kenneth Söderhäll, Irene Söderhäll

{"title":"The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis.","authors":"Thanapong Kruangkum, Kenneth Söderhäll, Irene Söderhäll","doi":"10.1007/s00441-024-03943-1","DOIUrl":"https://doi.org/10.1007/s00441-024-03943-1","url":null,"abstract":"The hematopoietic tissue (HPT) and anterior proliferation center (APC) are the main hemocyte-producing organs of the freshwater crayfish, Pacifastacus leniusculus. To deepen our understanding of immune responses to various pathogens, it is essential to identify distinct hemocyte subpopulations with specific functions and to further explore how these cells are generated. Here we provide an in-depth histological study of the HPT and APC in order to localize cell types in different developmental stages, and to provide some information regarding the hemocyte differentiation in the crayfish. We localized mRNA expression of previously identified genes in the HPT/APC and hemocytes by RNA-FISH. The expression of hemolectin and transglutaminase 1 was shown to be co-localized in a high number of the HPT cells, while transglutaminase 2 was expressed in different cell types mainly associated with epithelium or endothelium. Furthermore, by double RNA-FISH for hemolectin and a previously unidentified PDGF-like factor, combined with immunostaining for prophenoloxidase, we could identify several different subtypes of hemocytes, indicating that the immune function of hemocytes in crayfish is more diversified and complex than previously appreciated.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced cell survival in prepubertal testicular tissue cryopreserved with membrane lipids and antioxidants rich cryopreservation medium. 用富含膜脂和抗氧化剂的冷冻保存介质冷冻保存青春期前睾丸组织，可提高细胞存活率。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-01 Epub Date: 2024-11-25 DOI: 10.1007/s00441-024-03930-6

Reyon Dcunha, Anjana Aravind, Smitha Bhaskar, Sadhana Mutalik, Srinivas Mutalik, Sneha Guruprasad Kalthur, Anujith Kumar, Padmaraj Hegde, Satish Kumar Adiga, Yulian Zhao, Nagarajan Kannan, Thottethodi Subrahmanya Keshava Prasad, Guruprasad Kalthur

{"title":"Enhanced cell survival in prepubertal testicular tissue cryopreserved with membrane lipids and antioxidants rich cryopreservation medium.","authors":"Reyon Dcunha, Anjana Aravind, Smitha Bhaskar, Sadhana Mutalik, Srinivas Mutalik, Sneha Guruprasad Kalthur, Anujith Kumar, Padmaraj Hegde, Satish Kumar Adiga, Yulian Zhao, Nagarajan Kannan, Thottethodi Subrahmanya Keshava Prasad, Guruprasad Kalthur","doi":"10.1007/s00441-024-03930-6","DOIUrl":"10.1007/s00441-024-03930-6","url":null,"abstract":"The present study explores the advantages of enriching the freezing medium with membrane lipids and antioxidants in improving the outcome of prepubertal testicular tissue cryopreservation. For the study, testicular tissue from Swiss albino mice of prepubertal age group (2 weeks) was cryopreserved by slow freezing method either in control freezing medium (CFM; containing DMSO and FBS in DMEM/F12) or test freezing medium (TFM; containing soy lecithin, phosphatidylserine, phosphatidylethanolamine, cholesterol, vitamin C, sodium selenite, DMSO and FBS in DMEM/F12 medium) and stored in liquid nitrogen for at least one week. The tissues were thawed and enzymatically digested to assess viability, DNA damage, and oxidative stress in the testicular cells. The results indicate that TFM significantly mitigated freeze-thaw-induced cell death, DNA damage, and lipid peroxidation compared to tissue cryopreserved in CFM. Further, a decrease in Cyt C, Caspase-3, and an increase in Gpx4 mRNA transcripts were observed in tissues frozen with TFM. Spermatogonial germ cells (SGCs) collected from tissues frozen with TFM exhibited higher cell survival and superior DNA integrity compared to those frozen in CFM. Proteomic analysis revealed that SGCs experienced a lower degree of freeze-thaw-induced damage when cryopreserved in TFM, as evident from an increase in the level of proteins involved in mitigating the heat stress response, transcriptional and translational machinery. These results emphasize the beneficial role of membrane lipids and antioxidants in enhancing the cryosurvival of prepubertal testicular tissue offering a significant stride towards improving the clinical outcome of prepubertal testicular tissue cryopreservation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"97-117"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding on roles of pleckstrin homology-like domain family A member 1 protein. pleckstrin同源结构域家族A成员1蛋白的作用扩展。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-01 Epub Date: 2024-12-04 DOI: 10.1007/s00441-024-03942-2

Małgorzata Durbas

引用次数: 0

Mesonephric tubules expressing estrogen and androgen receptors remain in the rete ovarii of adult mice. 表达雌激素和雄激素受体的中肾小管仍然存在于成年小鼠的卵巢前叶。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-01 Epub Date: 2024-11-23 DOI: 10.1007/s00441-024-03931-5

Shiori Yoshimura, Takuya Omotehara, Hiroki Nakata, Lynn A Birch, Gail S Prins, Koichiro Ichimura, Masahiro Itoh

{"title":"Mesonephric tubules expressing estrogen and androgen receptors remain in the rete ovarii of adult mice.","authors":"Shiori Yoshimura, Takuya Omotehara, Hiroki Nakata, Lynn A Birch, Gail S Prins, Koichiro Ichimura, Masahiro Itoh","doi":"10.1007/s00441-024-03931-5","DOIUrl":"10.1007/s00441-024-03931-5","url":null,"abstract":"The rete ovarii and epoophoron in females are homologous structures of the rete testis and efferent/epididymal duct in males and are derived from the developing rete cells and mesonephric tubules, respectively. Sex steroid hormones play a critical role in reproductive function for both sexes, and we recently reported expression patterns of sex steroid receptors in developing male reproductive tracts. However, their expression patterns in females remain unclear. We, therefore, investigated the three-dimensional structure and expression patterns of sex steroid receptors in the rete ovarii and epoophoron of fetal and adult female mice. In adult females, the epoophoron was not adherent to the rete ovarii. The rete ovarii had a bursa-like structure, with its extra-ovarian region protruding toward the epoophoron. A marker for mesonephric tubules, PAX2 (Paired box 2), was detected in the epoophoron and a small population of epithelial cells in the extra-ovarian rete ovarii. These epithelial cells expressed estrogen receptor and androgen receptor. During development, mesonephric tubules were adherent to the rete ovarii at first, but as the development proceeded, the continuity was lost due to the interruption of the tubule rather than separation between the tip of the tubule and rete ovarii. These findings suggest that epithelial cells, originating from the mesonephric tubules, persist even in the adult rete ovarii with maintained expressions of receptors for estrogen and androgen.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"85-96"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-based genetic screens in human pluripotent stem cells derived neurons and brain organoids. 基于 CRISPR 技术对人类多能干细胞衍生的神经元和脑器官组织进行基因筛选。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-01 Epub Date: 2024-11-25 DOI: 10.1007/s00441-024-03934-2

Yu Guo, Xinyu Zhao

{"title":"CRISPR-based genetic screens in human pluripotent stem cells derived neurons and brain organoids.","authors":"Yu Guo, Xinyu Zhao","doi":"10.1007/s00441-024-03934-2","DOIUrl":"10.1007/s00441-024-03934-2","url":null,"abstract":"Recent large-scale genome-wide association and single-cell RNA sequencing (scRNA-seq) studies have uncovered disease-associated genetic risk factors and cell type-specific genetic alterations. However, our understanding of how these genetic variants cause diseases and the underlying mechanisms remains largely unknown. Functional genomics screens using CRISPR-based technologies offer an effective tool for studying genes relevant to disease phenotypes. Here, we summarize recent CRISPR-based functional genomics screen approaches applied to human pluripotent stem cell (hPSC)-derived neurons and brain organoids. These screens have identified genes crucial for neurogenesis, neuronal survival, morphological development, and migration. Combining CRISPR-based genetic screens with scRNA-seq, researchers have revealed downstream genes and cellular pathways impacted by these genetic variants in human neural cells, providing new insights into the pathogenesis of neurodevelopmental disorders, such as microcephaly and autism spectrum disorders. Finally, we discuss current challenges and future directions for using CRISPR-based screens in furthering our understanding of neurological diseases and developing potential therapeutic strategies. Despite challenges, CRISPR-based screens have enormous potential for advancing the therapeutic development of many diseases.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"1-8"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical characterization of interstitial cells and their spatial relationship to motor neurons within the mouse esophagus. 免疫组织化学鉴定小鼠食管间质细胞及其与运动神经元的空间关系。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-01-01 Epub Date: 2024-11-28 DOI: 10.1007/s00441-024-03929-z

Emer P Ni Bhraonain, Jack A Turner, Karen I Hannigan, Kenton M Sanders, Caroline A Cobine

{"title":"Immunohistochemical characterization of interstitial cells and their spatial relationship to motor neurons within the mouse esophagus.","authors":"Emer P Ni Bhraonain, Jack A Turner, Karen I Hannigan, Kenton M Sanders, Caroline A Cobine","doi":"10.1007/s00441-024-03929-z","DOIUrl":"10.1007/s00441-024-03929-z","url":null,"abstract":"Interstitial cells of Cajal (ICC) and PDGFRα+ cells regulate smooth muscle motility in the gastrointestinal (GI) tract, yet their function in the esophagus remains unknown. The mouse esophagus has been described as primarily skeletal muscle; however, ICC have been identified in this region. This study characterizes the distribution of skeletal and smooth muscle cells (SMCs) and their spatial relationship to ICC, PDGFRα+ cells, and intramuscular motor neurons in the mouse esophagus. SMCs occupied approximately 30% of the distal esophagus, but their density declined in more proximal regions. Similarly, ANO1+ intramuscular ICC (ICC-IM) were distributed along the esophagus, with density decreasing proximally. While ICC-IM were closely associated with SMCs, they were also present in regions of skeletal muscle. Intramuscular, submucosal, and myenteric PDGFRα+ cells were densely distributed throughout the esophagus, yet only intramuscular PDGFRα+ cells in the lower esophageal sphincter (LES) and distal esophagus expressed SK3. ICC-IM and PDGFRα+ cells were closely associated with intramuscular nNOS+, VIP+, VAChT+, and TH+ neurons and GFAP+ cells resembling intramuscular enteric glia. These findings suggest that ICC-IM and PDGFRα+ cells may have roles in regulating esophageal motility due to their close proximity to each other and to skeletal muscle and SMCs, although further functional studies are needed to explore their role in this region. The mixed muscular composition and presence of interstitial cells in the mouse distal esophagus is anatomically similar to the transitional zone found in the human esophagus, and therefore, motility studies in the mouse may be translatable to humans.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"61-84"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0