Cell and Tissue Research最新文献_第2页

Sucrose- and fat-related metabolic states influence the adaptation of the pulmonary lipid metabolism to hypoxia. 蔗糖和脂肪相关的代谢状态影响肺脂质代谢对缺氧的适应。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 DOI: 10.1007/s00441-025-03968-0

Sophia Pankoke, Lea Gerling, Matthias Ochs, Christian Mühlfeld, Julia Schipke

{"title":"Sucrose- and fat-related metabolic states influence the adaptation of the pulmonary lipid metabolism to hypoxia.","authors":"Sophia Pankoke, Lea Gerling, Matthias Ochs, Christian Mühlfeld, Julia Schipke","doi":"10.1007/s00441-025-03968-0","DOIUrl":"https://doi.org/10.1007/s00441-025-03968-0","url":null,"abstract":"Pulmonary surfactant is essential for lung function and consists mainly of lipids, almost half of which in adult mammals originate from de novo synthesis in alveolar epithelial type-2 (AE2) cells. Obesogenic nutrition and hypoxia coexist in obese patients with chronic lung diseases. This study tested the hypothesis that diet-induced obesity and chronic hypoxia alter lipid metabolism and thereby deteriorate surfactant homeostasis. Male C57BL/6N mice were fed control diet (4% fat, 6% sucrose; CD), high-sucrose diet (4% fat, 46% sucrose; HSD) or high-fat diet (35% fat, 7% sucrose; HFD). After 27 weeks, half of each diet group was exposed to hypoxia (13% O2, Hyp) for 3 weeks. After 30 weeks, lung mechanics were assessed, and the blood, livers, and lungs were analyzed. In CD-fed mice, hypoxia induced lung mechanical changes indicative of reduced elastic recoil properties, as well as smaller lamellar bodies (LBs) and higher composite body volumes, suggesting an increased surfactant precursor formation. HSD and HFD induced lipid accumulation in liver and AE2 cells. In HSD-Hyp and HFD-Hyp, LB volumes per alveolar surface area were elevated, indicating compensatory increases in intracellular surfactant pools which were absent in CD-Hyp. Additionally, hypoxia-related lung mechanics alterations were less pronounced in HSD-Hyp and HFD-Hyp. Lung proteome analysis revealed that only a few lipid metabolism-associated proteins were similarly regulated within diet groups under hypoxia, with the most prominent changes in sucrose-fed hypoxic animals. Thus, individual diet-related metabolic states specifically affect the adaptation of the pulmonary lipid metabolism and intracellular surfactant assembly to chronic hypoxia.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role and mechanism of Schwann cells in the repair of peripheral nerve injury. 雪旺细胞在周围神经损伤修复中的作用及机制。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-02-15 DOI: 10.1007/s00441-025-03957-3

Huiyue Xu, Zhipeng Fan

{"title":"The role and mechanism of Schwann cells in the repair of peripheral nerve injury.","authors":"Huiyue Xu, Zhipeng Fan","doi":"10.1007/s00441-025-03957-3","DOIUrl":"10.1007/s00441-025-03957-3","url":null,"abstract":"Limb injuries such as severe strains, deep cuts, gunshot wounds, and ischemia can cause peripheral nerve damage. This can result in a range of clinical symptoms including sensory deficits, limb paralysis and atrophy, neuralgia, and sweating abnormalities in the innervated areas affected by the damaged nerves. These symptoms can have a significant impact on patients' daily lives and work. Despite existing clinical treatments, some patients cannot achieve satisfactory therapeutic effects and continue to experience persistent paralysis and pain. Schwann cells are responsible for repairing and regenerating damaged nerves in the peripheral nervous system. They play a crucial role in the healing of nerve injuries and are essential for the restoration of proper nerve function. An increasing number of studies have focused on the various regulatory mechanisms that specifically affect the repair of damage by Schwann cells. This article aims to provide information on the different types of peripheral nerve injuries and their available treatments. We also discuss the various molecular mechanisms that regulate Schwann cell function during peripheral nerve repair and how they can be used to promote nerve repair and regeneration. Furthermore, we explore the potential therapeutic applications of precision regulation of Schwann cells for the treatment of peripheral nerve injuries.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"81-95"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recruitment of pulmonary intravascular macrophages in SARS-CoV-2 infected hamsters. SARS-CoV-2感染仓鼠肺血管内巨噬细胞的募集

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-02-27 DOI: 10.1007/s00441-025-03958-2

Carolina Rego Rodrigues, Gurpreet Kaur Aulakh, Andrea Kroeker, Swarali S Kulkarni, Jocelyne Lew, Darryl Falzarano, Baljit Singh

{"title":"Recruitment of pulmonary intravascular macrophages in SARS-CoV-2 infected hamsters.","authors":"Carolina Rego Rodrigues, Gurpreet Kaur Aulakh, Andrea Kroeker, Swarali S Kulkarni, Jocelyne Lew, Darryl Falzarano, Baljit Singh","doi":"10.1007/s00441-025-03958-2","DOIUrl":"10.1007/s00441-025-03958-2","url":null,"abstract":"The mechanisms by which severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe lung inflammation and mortality remain unclear. While the role of alveolar macrophages in COVID-19 is known, data on pulmonary intravascular macrophages (PIMs) is lacking. PIMs are key inflammatory cells present in species like cattle and pigs. Though constitutively absent in humans and rodents, their recruitment in rodents triggers exaggerated inflammation. We investigated the recruitment of PIMs and other immune cells, using immunofluorescence, hematoxylin and eosin (H&E) staining, and immunogold labeling in a hamster model of SARS-CoV-2 infection. Syrian golden hamsters were divided into 6 groups: uninfected control, unvaccinated-infected at 2-, 5-, and 14-days post infection (dpi) and vaccinated-infected at 5- and 14-dpi. Lung tissues were analyzed for neutrophils (myeloperoxidase), monocytes/macrophages (CCR2, CX3CR1), macrophages (IBA-1), and T cells (CD3). Septal macrophages increased at 2-, 5-, and 14-dpi in infected animals vs. control. CX3CR1 + cells decreased at 14-dpi in unvaccinated animals, but CX3CR1/CCR2 double positive cells were higher at 5-dpi, indicating a pro-inflammatory macrophage phenotype. PIMs were confirmed by transmission electron microscopy. These are the first data showing recruitment of pro-inflammatory PIMs in SARS-CoV-2 infected lungs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"1-15"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative stress in asthma pathogenesis: mechanistic insights and implications for airway smooth muscle dysfunction. 哮喘发病机制中的氧化应激：气道平滑肌功能障碍的机制见解和意义。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-02-07 DOI: 10.1007/s00441-025-03953-7

Kangxia Li, Xiang Ji, Shan Tian, Jian Li, Yizhu Tian, Xiaoqing Ma, Huanping Li, Hong Zhang, Cai-Tao Chen, Wei Gu

{"title":"Oxidative stress in asthma pathogenesis: mechanistic insights and implications for airway smooth muscle dysfunction.","authors":"Kangxia Li, Xiang Ji, Shan Tian, Jian Li, Yizhu Tian, Xiaoqing Ma, Huanping Li, Hong Zhang, Cai-Tao Chen, Wei Gu","doi":"10.1007/s00441-025-03953-7","DOIUrl":"10.1007/s00441-025-03953-7","url":null,"abstract":"Airway smooth muscle (ASM) dysfunction is a key factor in the narrowing of airways in asthma patients, characterized by excessive secretion of inflammatory factors, increased mass, and amplified contractile responses. These pathological features are instrumental in the propagation of airway inflammation, structural remodeling, and the escalation of airway hyperresponsiveness (AHR), which are also principal factors underlying the limitations of current therapeutic strategies. In asthmatic ASM, an imbalance between oxidant production and antioxidant defenses culminates in oxidative stress, which is involved in the excessive secretion of inflammatory factors, increased mass, and amplified contractile responses of ASM, and is a critical etiological factor implicated in the dysregulation of ASM function. The molecular pathways through which oxidative stress exerts its effects on ASM in asthma are multifaceted, with the Nrf2/HO-1, MAPK, and PI3K/Akt pathways being particularly noteworthy. These characteristic pathways play a potential role by connecting with different upstream and downstream signaling molecules and are involved in the amplification of ASM inflammatory responses, increased mass, and AHR. This review provides a comprehensive synthesis of the phenotypic expression of ASM dysfunction in asthma, the interplay between oxidants and antioxidants, and the evidence base and molecular underpinnings linking oxidative stress to ASM dysfunction. Given the profound implications of ASM dysfunction on the airflow limitation in asthma and the seminal role of oxidative stress in this process, a deeper exploration of these mechanisms is essential for unraveling the pathogenesis of asthma and may offer novel perspectives for its prophylaxis and management.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"17-34"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurological confirmation of periplanone-D exploitation as a primary sex pheromone and counteractions of other components in the smoky brown cockroach Periplaneta fuliginosa. 烟熏棕色蟑螂大蠊d作为主要性信息素的神经学证实及其他成分的拮抗作用。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-01-10 DOI: 10.1007/s00441-024-03935-1

Mana Domae, Masazumi Iwasaki, Hiroshi Nishino

{"title":"Neurological confirmation of periplanone-D exploitation as a primary sex pheromone and counteractions of other components in the smoky brown cockroach Periplaneta fuliginosa.","authors":"Mana Domae, Masazumi Iwasaki, Hiroshi Nishino","doi":"10.1007/s00441-024-03935-1","DOIUrl":"10.1007/s00441-024-03935-1","url":null,"abstract":"The smoky brown cockroach, Periplaneta fuliginosa, is a peridomestic pest inhabiting broad regions of the world from temperate to subtropical zones. In common with other related species such as the American cockroach, Periplaneta americana, female-emitted sex pheromone components, named periplanones, are known to be key volatiles that elicit long-range attraction and courtship rituals in males. How periplanones are processed in the nervous system has been entirely unexplored in P. fuliginosa. By using pheromone compounds, periplanones A, B, C, and D, as stimulants to the antenna, we identified four distinct types of interneurons (projection neurons) that relay pheromonal signals from a single olfactory glomerulus of the first-order olfactory center (antennal lobe) to higher-order centers in the ipsilateral hemibrain. All glomeruli innervated by pheromone-responsive projection neurons clustered near the antennal nerve entrance of the antennal lobe. The projection neuron with dendrites in the largest glomerulus was tuned specifically to periplanone-D, and adding other components to periplanone-D counteracted the excitation elicited by periplanone-D alone. Likewise, the projection neuron with dendrites in the second largest glomerulus and that with dendrites in a medium-sized glomerulus were tuned to periplanone-A and periplanone-B, respectively. Our results are, therefore, consistent with behavioral findings that periplanone-D alone acts as a primary sex attractant and that other components act as potential behavioral antagonists. Moreover, a comparison of the glomeruli in P. fuliginosa and P. americana suggested that there are differences in the sizes of homologous glomeruli, as well as in the ligands they process.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"51-70"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Neurological confirmation of periplanone-D exploitation as a primary sex pheromone and counteractions of other components in the smoky brown cockroach Periplaneta fuliginosa. 修正：烟熏棕色蟑螂大蠊d作为主要性信息素的利用和其他成分的对抗作用的神经学证实。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 DOI: 10.1007/s00441-025-03951-9

Mana Domae, Masazumi Iwasaki, Hiroshi Nishino

引用次数: 0

Histological study on the postnatal development of the nerve network in the rat ileal mucosa and submucosa. 大鼠回肠黏膜及粘膜下层神经网络出生后发育的组织学研究。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-02-13 DOI: 10.1007/s00441-025-03949-3

Rinako Morishita, Satoki Nakanishi, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani

{"title":"Histological study on the postnatal development of the nerve network in the rat ileal mucosa and submucosa.","authors":"Rinako Morishita, Satoki Nakanishi, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani","doi":"10.1007/s00441-025-03949-3","DOIUrl":"10.1007/s00441-025-03949-3","url":null,"abstract":"We have previously reported detailed structures of the mucosal nerve network in the rat ileum, but the mechanisms underlying the development of this nerve network remain unclear. Therefore, we aimed to clarify the developmental process of the mucosal nerve network and submucosal neurons (SM-neurons) or ganglia (SMG), which are the main source of nerve fibers projected to the mucosa, in the rat ileum during the postnatal period. Immunohistochemistry against tubulin beta III (Tuj1) revealed that Tuj1-immunopositivities were more abundant in the lamina propria at 2 weeks old (2wk; pre-weaning) than at postnatal day 0 (P0) or 4 weeks old (4wk; post-weaning) and more frequent on the mesenteric side than on the antimesenteric side at 2wk. Hu antigen D (HuD)-immunopositive SM-neurons and SMG were also more abundantly localized on the mesenteric side than the antimesenteric side at P0 and 2wk. On the other hand, cells immunopositive for SRY-related HMG-box 10 (Sox10), which is the marker for enteric nervous system progenitor cells and enteric glial cells, were homogenously scattered in the submucosa throughout the entire circumference at all ages. Glial cell marker S100 calcium-binding protein B (S100β) in the submucosa was detected at all ages without any significant difference between the mesenteric and antimesenteric sides. These findings indicate that SMG formation and associated neurite extension into the mucosa in the rat ileum might occur preferentially on the mesenteric side by the weaning period, leading us to hypothesize that the mechanism by which the mucosal nerve network and SMG develop differs along the mesenteric-antimesenteric side axis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"71-80"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11965212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity-driven musculotendinous remodeling impairs tissue resilience to mechanical damage. 肥胖驱动的肌肉肌腱重塑损害组织对机械损伤的恢复力。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-31 DOI: 10.1007/s00441-025-03967-1

Cesanelli L, Minderis P, Balnyte I, Ratkevicius A, Degens H, Satkunskiene D

{"title":"Obesity-driven musculotendinous remodeling impairs tissue resilience to mechanical damage.","authors":"Cesanelli L, Minderis P, Balnyte I, Ratkevicius A, Degens H, Satkunskiene D","doi":"10.1007/s00441-025-03967-1","DOIUrl":"https://doi.org/10.1007/s00441-025-03967-1","url":null,"abstract":"Obesity has been associated with lower muscle strength-to-body mass ratio. Here, we evaluated the effects of diet-induced obesity on the mechano-structural properties of isolated muscles and tendons. Thirty 10-week-old male C57BL/6 J mice were randomly assigned to either an obesogenic high-fat diet group (OB) for 24 weeks or a control group (CN) maintained on a standard chow diet. Soleus muscle (SOL) and Achilles tendon (AT) specimens were isolated and subjected either to failure testing, 300 cycles of passive stretch-destretch, or isometric twitch contractions. Morpho-structural and protein expression analyses were conducted to assess collagen and adipose tissue accumulation, concentrations of cross-linking factors, and any alterations in the POSTN-TGFβ1-Akt signaling pathway. OB SOL and AT tissues were more fragile than those from CN (p < 0.05). A piecewise linear regression model revealed a tendency for OB tissues to exhibit steeper mechanical property changes within the first 20 cycles compared to CN, followed by a similar plateau phase in both groups. OB SOL-AT complexes showed a slower twitch-contraction-relaxation pattern than CN (p < 0.05). OB tendons and muscles were larger than those of the CN, with muscles featuring bigger fibers, and higher collagen area fraction (p < 0.05). Elevated TGFβ1 and POSTN concentrations were observed in OB tissues (p < 0.05), alongside increased P-Akt and P-4EBP1 expression (p < 0.05). These findings highlight the detrimental effects of obesity on the structural integrity of muscle and tendon tissues and suggest a significant role of POSTN-TGFβ1-Akt signaling in obesity-associated musculotendinous remodeling.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attenuation of intestinal peristalsis with age is attributed to decreased sensitivity of receptors in the enteric nervous system. 随着年龄的增长，肠蠕动的衰减是由于肠神经系统中受体的敏感性降低。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-27 DOI: 10.1007/s00441-025-03969-z

Tsukasa Kobayashi, Yuko Takeba, Masanori Ootaki, Yuki Ohta, Keisuke Kida, Taroh Iiri, Naoki Matsumoto

{"title":"Attenuation of intestinal peristalsis with age is attributed to decreased sensitivity of receptors in the enteric nervous system.","authors":"Tsukasa Kobayashi, Yuko Takeba, Masanori Ootaki, Yuki Ohta, Keisuke Kida, Taroh Iiri, Naoki Matsumoto","doi":"10.1007/s00441-025-03969-z","DOIUrl":"https://doi.org/10.1007/s00441-025-03969-z","url":null,"abstract":"Constipation and other digestive disorders are common in older adults. The autonomic nervous system plays a critical role in regulating digestive motility in the intestinal tract. However, studies on age-related changes in autonomic function and receptor expression in the intestinal tract are limited. In this study, we examined the expression of neurotransmitter receptors in the autonomic nervous system and the effects of acetylcholine and β-agonists on intestinal contraction and relaxation in the jejunum of aged rats. Jejunal sections collected from male and female Wistar/ST rats aged 4, 11, and 18 months were analyzed. Immunohistochemical staining and enzyme-linked immunosorbent assay were used to measure the expression of muscarinic acetylcholine receptors (CHRM2 and CHRM3) and β-adrenergic receptors (β2-ADR and β3-ADR). The effects of acetylcholine, isoproterenol, and mirabegron were assessed in the isolated jejunum for each age group. There was no significant difference in CHRM2 receptor expression among the age groups; however, CHRM3 receptor expression decreased with age. Additionally, the sensitivity to acetylcholine-induced contractile responses decreased with age. Although β2-ADR receptor expression did not differ among the age groups, β3-ADR receptor expression increased with age. Despite this, the relaxation response to isoproterenol and mirabegron decreased with age. Our study revealed an age-related decrease in CHRM3 expression and the contractile response to acetylcholine in the small intestine of rats. Although β-ADR expression, particularly β3-ADR, increased with age, the relaxation response to β-adrenergic agonists gradually decreased.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The large milkweed bugs' Na,K-ATPase β-subunits colocalize with septate junction proteins in a tissue-specific manner. 大型乳草虫的Na， k - atp酶β-亚基以组织特异性的方式与分离的连接蛋白共定位。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-26 DOI: 10.1007/s00441-025-03965-3

Marlena Herbertz, Christian Lohr, Susanne Dobler

{"title":"The large milkweed bugs' Na,K-ATPase β-subunits colocalize with septate junction proteins in a tissue-specific manner.","authors":"Marlena Herbertz, Christian Lohr, Susanne Dobler","doi":"10.1007/s00441-025-03965-3","DOIUrl":"https://doi.org/10.1007/s00441-025-03965-3","url":null,"abstract":"The Na,K-ATPase is a vital transmembrane enzyme, which is important for maintaining cell membrane potentials and the general functionality of animal cells. The enzyme's minimal functional unit consists of one α and one β-subunit, whereas the number of existing paralogs varies in different insect species. The functional roles of different β-subunits, which can account for their diversity within a single species, are so far only partially explained. The emphasis of this study was to specifically elucidate the involvement in septate junctions of the four β-subunits of the new model system Oncopeltus fasciatus. Septate junctions function as a paracellular barrier controlling the flow of solutes across epithelia. So far, studies in Drosophila revealed that nervana2, the β2 homolog of Drosophila, is involved in septate junction formation. In O. fasciatus, we demonstrate that most of the Na,K-ATPase subunits colocalize with septate junction proteins. This agrees with our previous findings implying a role of β2 in the control of tracheal tube size in O. fasciatus, which according to the findings in Drosophila appears to be dependent on a stable formation of septate junctions. Finally, our data suggest a connection between the septate junction protein coracle and the enigmatic, N-terminally strongly truncated βx, which has no obvious homologs in other insects. Our study proposes that the four β-subunits form functional units with septate junction proteins, either allowing tissue-adjusted formation of cell-cell contacts or other yet unknown functions.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0