Cell and Tissue Research最新文献_第2页

Phagocytic hemocytes, independent of pericardial cells, modulate cellular immune responses on the dorsal vessel of mosquitoes, including the infection-induced reduction of the heart rate. 独立于心包细胞的吞噬血细胞调节蚊子背血管上的细胞免疫反应，包括感染引起的心率降低。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-19 DOI: 10.1007/s00441-025-04011-y

Cole J Meier, Shabbir Ahmed, Tania Y Estévez-Lao, Julián F Hillyer

{"title":"Phagocytic hemocytes, independent of pericardial cells, modulate cellular immune responses on the dorsal vessel of mosquitoes, including the infection-induced reduction of the heart rate.","authors":"Cole J Meier, Shabbir Ahmed, Tania Y Estévez-Lao, Julián F Hillyer","doi":"10.1007/s00441-025-04011-y","DOIUrl":"https://doi.org/10.1007/s00441-025-04011-y","url":null,"abstract":"Infection induces the aggregation of hemocytes on the dorsal vessel of mosquitoes. These hemocytes, called periostial hemocytes, phagocytose pathogens and produce immune factors on the abdominal portion of the dorsal vessel, called the heart. One of these immune factors, nitric oxide, is a pleiotropic free radical that is an antimicrobial and a heartbeat reducer. But nitric oxide is not just produced by hemocytes. It is also synthesized by pericardial cells that flank the heart, and other tissues. To determine whether it is the periostial hemocytes or the pericardial cells that modulate the heart following infection, we chemically ablated the hemocytes using clodronate liposomes and measured immune responses and heart physiology. We demonstrate that clodronate liposomes ablate the sessile hemocytes, including the periostial hemocytes, while leaving the pericardial cells and heart integrity unaffected. Moreover, ablating hemocytes abolishes the phagocytosis of bacteria, alters the deposition of melanized bacteria, and decreases nitric oxide synthase activity on the heart. Importantly, hemocyte ablation eliminates the infection induced reduction of the heart rate, mainly by modifying the anterograde heart rate. Therefore, periostial hemocytes drive immune responses on the heart and infection-induced changes to circulatory physiology.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catecholaminergic system in the brain of the terrestrial slug Limax valentianus. 陆生蛞蝓脑内的儿茶酚胺能系统。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-19 DOI: 10.1007/s00441-025-04010-z

Ryota Matsuo, Aika Matsuki, Yuko Matsuo

{"title":"Catecholaminergic system in the brain of the terrestrial slug Limax valentianus.","authors":"Ryota Matsuo, Aika Matsuki, Yuko Matsuo","doi":"10.1007/s00441-025-04010-z","DOIUrl":"https://doi.org/10.1007/s00441-025-04010-z","url":null,"abstract":"Catecholamines, including dopamine, are thought to play important roles in the nervous system of mollusks. In the brains of the terrestrial gastropods, the procerebrum is the higher olfactory center involved in olfactory associative learning. Dopamine is known to affect the activities of interneurons in the procerebrum. However, little is known about from where and how dopaminergic neurons project to the procerebrum. In the present study, we generated a specific antibody against tyrosine hydroxylase (TH) and visualized catecholaminergic neurons, including dopaminergic neurons, in the central and peripheral nervous systems of the terrestrial slug Limax valentianus. We found that (1) the number of the cell bodies of catecholaminergic neurons was larger in the ventral aspect than in the dorsal aspect of the brain; (2) most of the TH-immunoreactive putative sensory afferents in the superior tentacle projected to the brain along the axis as bundles in a regularly spaced manner while bypassing the procerebrum following entry into the brain; (3) TH-immunoreactive nerves in the procerebrum were derived from at least three distinct origins outside and within the procerebrum; and (4) dopamine upregulated the oscillatory frequency of the local field potential oscillation in the procerebrum, which was occluded by pre-incubation with sulpiride, a mammalian D2/D3 receptor antagonist. This is the first study to investigate the catecholaminergic system, with a special focus on the procerebrum, using a specific antibody against TH in terrestrial gastropods. The present study uncovered catecholaminergic regulation of procerebrum activity through innervation from both the outside and inside of the procerebrum.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the antiangiogenic effects of Phospholipases A₂ from Bothrops diporus venom. 探讨双翅虫毒液磷脂酶A2的抗血管生成作用。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-10 DOI: 10.1007/s00441-025-04008-7

Daniela J Sasovsky, Gabriela B Olea, Gonzalo Ojeda, Angélica M Cesario, Franco J Gonzalez, Bruno Lomonte, Daniel Lombardo, Soledad Bustillo

{"title":"Exploring the antiangiogenic effects of Phospholipases A2 from Bothrops diporus venom.","authors":"Daniela J Sasovsky, Gabriela B Olea, Gonzalo Ojeda, Angélica M Cesario, Franco J Gonzalez, Bruno Lomonte, Daniel Lombardo, Soledad Bustillo","doi":"10.1007/s00441-025-04008-7","DOIUrl":"https://doi.org/10.1007/s00441-025-04008-7","url":null,"abstract":"Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a crucial process in both physiological and pathological contexts, including cancer. Phospholipases A2 (PLA2s), enzymes found in snake venoms, have attracted attention due to their potential antiangiogenic properties. In this study, we explored the antiangiogenic effects of PLA2 isoforms isolated from Bothrops diporus venom using a combination of in vivo and ex vivo models. The chorioallantoic membrane (CAM) assay revealed a significant reduction in vascular density and branching following PLA2s treatment, with histological analysis confirming vascular regression, including vessel wall thinning and luminal collapse. Moreover, PLA2s induced endothelial cell apoptosis, as shown by TUNEL staining, and reduced VEGF expression. The filter paper disc variant of the CAM assay further supported these findings, demonstrating inhibited neovascularization while preserving mature vessels. Additionally, the CAM explant assay showed a marked decrease in vascular complexity and branching. These results demonstrate the antiangiogenic effect of PLA2 isoforms from B. diporus and suggest that these enzymes may modulate key angiogenic pathways. Based on our previous findings, this modulation may involve interference with integrin-mediated signaling, which could underlie the vascular effects observed. Thus, this work provides compelling evidence for the potential role of snake venom-derived PLA2s in modulating angiogenesis and highlights the need for further research into their mechanisms and possible biomedical applications.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note to: Overexpression of miR-429 impairs intestinal barrier function in diabetic mice by down-regulating occludin expression. 注：过表达miR-429通过下调occludin表达损害糖尿病小鼠肠道屏障功能。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-04 DOI: 10.1007/s00441-025-04009-6

Tao Yu, Xi-Ji Lu, Jie-Yao Li, Ti-Dong Shan, Can-Ze Huang, Hui Ouyang, Hong-Sheng Yang, Ji-Hao Xu, Wa Zhong, Zhong-Sheng Xia, Qi-Kui Chen

引用次数: 0

Parvin is essential for indirect flight muscle (IFM) performance and Zasp52 localisation to the Z-disc in Drosophila melanogaster. Parvin对于果蝇的间接飞行肌肉（IFM）表现和Zasp52在z盘的定位至关重要。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-03 DOI: 10.1007/s00441-025-04007-8

Ushashi Ain, Benazir Fatma, Hena Firdaus

{"title":"Parvin is essential for indirect flight muscle (IFM) performance and Zasp52 localisation to the Z-disc in Drosophila melanogaster.","authors":"Ushashi Ain, Benazir Fatma, Hena Firdaus","doi":"10.1007/s00441-025-04007-8","DOIUrl":"10.1007/s00441-025-04007-8","url":null,"abstract":"The integrin-associated proteins (IAPs) function in a tightly regulated and coordinated manner to maintain the complex cytoarchitecture at the myotendinous junctions (MTJs) of Drosophila indirect flight muscles (IFMs). Parvin, a conserved but less explored IAP, forms a ternary complex with ILK and PINCH (the IPP complex). Although the IPP complex is functionally conserved, playing a central role in integrin-mediated adhesion, its individual components may also exert independent roles. The present study investigates parvin as a critical regulator of IFM function and sarcomeric integrity. Downregulation of parvin in IFM leads to altered sarcomere organisation and lowered accumulation of essential thin filament genes, including Act88F, wupA, up, TpnC4, and TM2 transcripts. Furthermore, Z-disc-associated proteins such as Zasp52 and its binding partner α-actinin, which are vital for myofibril stability, showed markedly reduced expression in parvin-deficient muscles. Notably, Zasp52 failed to localise to the Z-discs in IFMs, despite being detectable in leg muscles, suggesting tissue-specific mislocalisation. The expression of sallimus, a titin orthologue contributing to muscle elasticity, remained unchanged. Our findings underscore parvin's essential role in preserving IFM ultrastructure and function. We also emphasise the importance of maintaining the stoichiometric balance within the IPP complex-including its extended member Ras suppressor 1 (RSU1)-for proper muscle performance. Additionally, perturbation of parvin expression in a tissue-specific manner revealed its broader role in fly viability and muscle-driven behaviours, including larval locomotion and leg muscle function. Collectively, this study positions parvin as a pivotal component in maintaining muscle integrity across multiple muscle types in Drosophila.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes. 更正：β -阻滞蛋白1参与小鼠软骨细胞中透明质酸降解刺激的分解代谢反应。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 DOI: 10.1007/s00441-025-03990-2

引用次数: 0

Involvement of connexin 43 in myocardial ischemia-reperfusion injury. 连接蛋白43在心肌缺血再灌注损伤中的作用。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-06-19 DOI: 10.1007/s00441-025-03987-x

Marisol Ruiz-Meana, Javier Inserte, Marta Consegal, Antonio Rodríguez-Sinovas

{"title":"Involvement of connexin 43 in myocardial ischemia-reperfusion injury.","authors":"Marisol Ruiz-Meana, Javier Inserte, Marta Consegal, Antonio Rodríguez-Sinovas","doi":"10.1007/s00441-025-03987-x","DOIUrl":"10.1007/s00441-025-03987-x","url":null,"abstract":"Connexin 43 (Cx43) is the principal connexin isoform expressed in the ventricular myocardium, where it is critically involved in the pathophysiology of cardiac ischemia-reperfusion injury. Its functions in this pathological condition span at least three different fronts. First, Cx43-mediated gap junctional channels contribute to the spread of cellular damage during reperfusion, allowing the transfer of sodium ions between injured and surviving cardiomyocytes. Further, under ischemic conditions, unapposed Cx43 hemichannels exacerbate injury by promoting calcium overload, metabolite losses, and membrane potential instability. Additionally, recent evidence suggest that mitochondrial Cx43 influences oxidative stress by modulating reactive oxygen species generation through the regulation of reverse electron transfer (RET) at the mitochondrial electron transport chain. These detrimental roles of Cx43 in acute myocardial ischemia-reperfusion injury, together with its previously described involvement in ischemic preconditioning, emphasize the dual functionality and importance of Cx43 in the context of acute myocardial infarction. The scope of this review is to summarize the current knowledge on the different mechanisms by which Cx43 promotes cell damage during myocardial infarction, with special emphasis on the regulation of RET.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"297-309"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical relationships of huntingtin-associated protein 1 with choline acetyltransferase in the forebrain cholinergic nuclei of adult mice. 亨廷顿蛋白相关蛋白1与成年小鼠前脑胆碱能核胆碱乙酰转移酶的免疫组化关系。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-07-19 DOI: 10.1007/s00441-025-03996-w

Mirza Mienur Meher, Md Nabiul Islam, Akie Yanai, Marya Afrin, Mir Rubayet Jahan, Kanako Nozaki, Koh-Hei Masumoto, Koh Shinoda

{"title":"Immunohistochemical relationships of huntingtin-associated protein 1 with choline acetyltransferase in the forebrain cholinergic nuclei of adult mice.","authors":"Mirza Mienur Meher, Md Nabiul Islam, Akie Yanai, Marya Afrin, Mir Rubayet Jahan, Kanako Nozaki, Koh-Hei Masumoto, Koh Shinoda","doi":"10.1007/s00441-025-03996-w","DOIUrl":"10.1007/s00441-025-03996-w","url":null,"abstract":"Huntingtin-associated protein 1 (HAP1) is a core component of the stigmoid body (STB) and a neuroprotective interactor with the causative agents of several neurodegenerative diseases (NDs). The cholinergic system is often affected by NDs. Our previous studies suggest that cholinergic brainstem/spinal cord motoneurons are more vulnerable to neurodegeneration due to a lack of STB/HAP1 protectivity. The forebrain cholinergic nuclei are also major neurodegenerative/psychotic targets; however, the relationships of HAP1 with choline acetyltransferase (ChAT) have yet to be determined there. This study used western blotting and immunohistochemistry to evaluate the comparative distribution of HAP1 with ChAT and their immunohistochemical relationships in the adult mouse forebrain cholinergic nuclei. The results showed that HAP1-immunoreactive (ir) neurons were highly distributed in the basal forebrain cholinergic nuclei, including medial septal nucleus (MS), nucleus of vertical limb of the diagonal band of Broca (VDB), nucleus of horizontal limb of the diagonal band of Broca (HDB), and substantia innominata basal part (SIB). HAP1-ir neurons were sporadically scattered in the striatum. The significantly highest co-expression ratio of HAP1 with ChAT was observed in MS and VDB. In contrast, the ChAT-ir neurons never contained HAP1 in the caudate putamen of the striatum. These suggest that, due to having putative HAP1 protectivity, the cholinergic neurons in MS and VDB that are mainly projected to the hippocampal/parahippocampal regions might be protected to regulate social memory formation, emotion, and other psychological functions. Consequently, the lack of HAP1 protectivity might make cholinergic neurons in the striatum more prone to neurodegeneration in certain NDs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"237-263"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Male reproductive system in Aegla (Decapoda: Aeglidae) and its anatomic-histological and ultrastructural relationship with other anomuran crabs. 海蟹（十足目：海蟹科）雄性生殖系统及其与其他异形蟹的解剖组织学和超微结构关系。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-07-18 DOI: 10.1007/s00441-025-03993-z

Bárbara Paiva, Caio Santos Nogueira, Gustavo Monteiro Teixeira, Fernando José Zara

{"title":"Male reproductive system in Aegla (Decapoda: Aeglidae) and its anatomic-histological and ultrastructural relationship with other anomuran crabs.","authors":"Bárbara Paiva, Caio Santos Nogueira, Gustavo Monteiro Teixeira, Fernando José Zara","doi":"10.1007/s00441-025-03993-z","DOIUrl":"10.1007/s00441-025-03993-z","url":null,"abstract":"The male reproductive system (MRS) of decapods in the genus Aegla remains poorly understood from both histological and ultrastructural perspectives. This study provides a comparative description of the anatomy, histology, and ultrastructure of the MRS in multiple Aegla species, with the aim of exploring their phylogenetic relationships with representatives of the superfamilies Lomisoidea and Chirostyloidea. Anatomically, the MRS of Aegla is located in the cephalothorax and consists of a bilateral structure. The testes are connected by a central commissure and independently open into each vas deferens. The vas deferens is a translucent tube subdivided into proximal, medial, and distal regions. Across all regions, the seminal fluid contains few free spermatozoa, and spermatophores are absent. This fluid comprises two types of secretion: type I (basophilic) and type II (strongly basophilic), both composed mainly of proteins and acidic polysaccharides, with interspecific variation in acidic polysaccharide content. Aegla spermatozoa exhibit a standard organization, organized into two hemispheres-cytoplasmic and nuclear-and possess an acrosome vesicle with two concentric layers. Comparatively, their ultrastructure closely resembles that of Lomis hirta, whereas members of Chirostyloidea lack similar features, indicating an evolutionary divergence. These findings contribute important insights into the evolutionary history of Anomura, highlighting the absence of spermatophores in Aegla-a condition typical of this infraorder-and underscoring the similarity in spermatozoa ultrastructure between Aegla and Lomis, likely reflecting a shared ancestral trait.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"319-340"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue. 脂肪组织去泛素化酶CYLD失活小鼠的表型分析。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-09-01 Epub Date: 2025-07-02 DOI: 10.1007/s00441-025-03989-9

Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos

{"title":"Phenotypic analysis of mice with inactivation of the deubiquitinating enzyme CYLD in adipose tissue.","authors":"Christos Gonidas, Theofilos Poutahidis, Athanasios Siasiaridis, Doxakis Anestakis, Polyanthi Konstantinidou, Anastasia Tsingotjidou, Sofia Gargani, George Mosialos","doi":"10.1007/s00441-025-03989-9","DOIUrl":"10.1007/s00441-025-03989-9","url":null,"abstract":"The cylindromatosis tumor suppressor (CYLD) is a deubiquitinating enzyme that has been implicated in lipid metabolism. More specifically, CYLD has been associated with lipid homeostasis in Drosophila melanogaster, and CYLD deficiency in mammals has been linked to dysregulation of lipid metabolism in the liver. Comprehensive tissue RNA expression analyses have revealed comparable levels of Cyld mRNA expression in the adipose tissue and liver, the organs that, together with skeletal muscle, primarily regulate lipid homeostasis. In the present study, the role of CYLD in mammalian adipose tissue homeostasis and function was investigated, utilizing a relevant conditional mouse model of CYLD inactivation that permits tissue-specific elimination of the catalytic domain of CYLD. Mutant mice displayed reduced weight-gain rate compared to controls when fed a normal or high-fat diet. Histological analysis of crown-like structures (CLS) indicated a reduced inflammatory response in the white adipose tissue of mutants. Our data collectively demonstrate that CYLD plays a pivotal role in regulating key metabolic parameters and modulating inflammatory responses within adipose tissue.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"311-317"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0