Cell and Tissue Research最新文献_第2页

Retraction Note to: Preconditioning with melatonin improves therapeutic outcomes of bone marrow-derived mesenchymal stem cells in targeting liver fibrosis induced by CCl4.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-17 DOI: 10.1007/s00441-025-03959-1

Keywan Mortezaee, Neda Khanlarkhani, Fatemeh Sabbaghziarani, Saeid Nekoonam, Jamal Majidpoor, Amir Hosseini, Parichehr Pasbakhsh, Iraj Ragerdi Kashani, Adib Zendedel

引用次数: 0

Identification of glutamate-related disease-dependent alterations in subventricular NSCs of the 3xTg Alzheimer's disease model, could they be involved in attempting damage repair?

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-17 DOI: 10.1007/s00441-025-03954-6

Giorgia Cerqueni, Valentina Terenzi, Alessandra Preziuso, Tiziano Serfilippi, Silvia Piccirillo, Mariangela Di Vincenzo, Patrizia Ambrogini, Salvatore Amoroso, Monia Orciani, Vincenzo Lariccia, Simona Magi

{"title":"Identification of glutamate-related disease-dependent alterations in subventricular NSCs of the 3xTg Alzheimer's disease model, could they be involved in attempting damage repair?","authors":"Giorgia Cerqueni, Valentina Terenzi, Alessandra Preziuso, Tiziano Serfilippi, Silvia Piccirillo, Mariangela Di Vincenzo, Patrizia Ambrogini, Salvatore Amoroso, Monia Orciani, Vincenzo Lariccia, Simona Magi","doi":"10.1007/s00441-025-03954-6","DOIUrl":"https://doi.org/10.1007/s00441-025-03954-6","url":null,"abstract":"Alzheimer's disease (AD) is an age-related neurodegenerative disorder characterised by several factors, such as impaired glutamate neurotransmission affecting crucial functions. Neural stem cells (NSCs) are present in the adult brains of all mammalian species and contribute to the continuous generation of neural cells throughout life. The disruption of glutamate levels during the development of AD could impact NSCs' functionality, influencing their response to the microenvironment. In this work, we isolated adult neural stem cells from both triple transgenic (3xTg)-AD mice and age-matched wild type (WT) mice in order to gather information on any differences between them, particularly concerning the potential mechanisms involved in the internalisation of glutamate and its utilisation for energy production. The 3xTg model offers the ability to recapitulate human pathology with both plaque and tangle hallmarks that are involved in the process of glutamate release. In vitro culture 3xTg NSCs showed a slight morphological difference compared to WT cells and a massive reduction of proliferation and viability. Furthermore, 3xTg NSCs displayed an increase in the expression of glutamate transporters and glutamine synthetase, while glutamate dehydrogenase did not show any reduction, which is typical in AD brains. Data obtained from this basic research study suggest a possible involvement of glutamate in the cellular energy balance, indicating an attempted response of NSCs to the cytotoxic microenvironment in the early stage of AD pathology. This finding is of great interest, as it corroborates the hypothesis that targeting the glutamatergic system could be an extremely promising strategy for new therapeutics in AD.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role and mechanism of Schwann cells in the repair of peripheral nerve injury.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-15 DOI: 10.1007/s00441-025-03957-3

Huiyue Xu, Zhipeng Fan

{"title":"The role and mechanism of Schwann cells in the repair of peripheral nerve injury.","authors":"Huiyue Xu, Zhipeng Fan","doi":"10.1007/s00441-025-03957-3","DOIUrl":"https://doi.org/10.1007/s00441-025-03957-3","url":null,"abstract":"Limb injuries such as severe strains, deep cuts, gunshot wounds, and ischemia can cause peripheral nerve damage. This can result in a range of clinical symptoms including sensory deficits, limb paralysis and atrophy, neuralgia, and sweating abnormalities in the innervated areas affected by the damaged nerves. These symptoms can have a significant impact on patients' daily lives and work. Despite existing clinical treatments, some patients cannot achieve satisfactory therapeutic effects and continue to experience persistent paralysis and pain. Schwann cells are responsible for repairing and regenerating damaged nerves in the peripheral nervous system. They play a crucial role in the healing of nerve injuries and are essential for the restoration of proper nerve function. An increasing number of studies have focused on the various regulatory mechanisms that specifically affect the repair of damage by Schwann cells. This article aims to provide information on the different types of peripheral nerve injuries and their available treatments. We also discuss the various molecular mechanisms that regulate Schwann cell function during peripheral nerve repair and how they can be used to promote nerve repair and regeneration. Furthermore, we explore the potential therapeutic applications of precision regulation of Schwann cells for the treatment of peripheral nerve injuries.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological study on the postnatal development of the nerve network in the rat ileal mucosa and submucosa.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-13 DOI: 10.1007/s00441-025-03949-3

Rinako Morishita, Satoki Nakanishi, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani

{"title":"Histological study on the postnatal development of the nerve network in the rat ileal mucosa and submucosa.","authors":"Rinako Morishita, Satoki Nakanishi, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani","doi":"10.1007/s00441-025-03949-3","DOIUrl":"https://doi.org/10.1007/s00441-025-03949-3","url":null,"abstract":"We have previously reported detailed structures of the mucosal nerve network in the rat ileum, but the mechanisms underlying the development of this nerve network remain unclear. Therefore, we aimed to clarify the developmental process of the mucosal nerve network and submucosal neurons (SM-neurons) or ganglia (SMG), which are the main source of nerve fibers projected to the mucosa, in the rat ileum during the postnatal period. Immunohistochemistry against tubulin beta III (Tuj1) revealed that Tuj1-immunopositivities were more abundant in the lamina propria at 2 weeks old (2wk; pre-weaning) than at postnatal day 0 (P0) or 4 weeks old (4wk; post-weaning) and more frequent on the mesenteric side than on the antimesenteric side at 2wk. Hu antigen D (HuD)-immunopositive SM-neurons and SMG were also more abundantly localized on the mesenteric side than the antimesenteric side at P0 and 2wk. On the other hand, cells immunopositive for SRY-related HMG-box 10 (Sox10), which is the marker for enteric nervous system progenitor cells and enteric glial cells, were homogenously scattered in the submucosa throughout the entire circumference at all ages. Glial cell marker S100 calcium-binding protein B (S100β) in the submucosa was detected at all ages without any significant difference between the mesenteric and antimesenteric sides. These findings indicate that SMG formation and associated neurite extension into the mucosa in the rat ileum might occur preferentially on the mesenteric side by the weaning period, leading us to hypothesize that the mechanism by which the mucosal nerve network and SMG develop differs along the mesenteric-antimesenteric side axis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gross anatomy of the visual processing centers of Hieroglyphus banian.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-12 DOI: 10.1007/s00441-025-03956-4

Sivaraju C, Joby Joseph

引用次数: 0

Oxidative stress in asthma pathogenesis: mechanistic insights and implications for airway smooth muscle dysfunction.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-07 DOI: 10.1007/s00441-025-03953-7

Kangxia Li, Xiang Ji, Shan Tian, Jian Li, Yizhu Tian, Xiaoqing Ma, Huanping Li, Hong Zhang, Cai-Tao Chen, Wei Gu

{"title":"Oxidative stress in asthma pathogenesis: mechanistic insights and implications for airway smooth muscle dysfunction.","authors":"Kangxia Li, Xiang Ji, Shan Tian, Jian Li, Yizhu Tian, Xiaoqing Ma, Huanping Li, Hong Zhang, Cai-Tao Chen, Wei Gu","doi":"10.1007/s00441-025-03953-7","DOIUrl":"https://doi.org/10.1007/s00441-025-03953-7","url":null,"abstract":"Airway smooth muscle (ASM) dysfunction is a key factor in the narrowing of airways in asthma patients, characterized by excessive secretion of inflammatory factors, increased mass, and amplified contractile responses. These pathological features are instrumental in the propagation of airway inflammation, structural remodeling, and the escalation of airway hyperresponsiveness (AHR), which are also principal factors underlying the limitations of current therapeutic strategies. In asthmatic ASM, an imbalance between oxidant production and antioxidant defenses culminates in oxidative stress, which is involved in the excessive secretion of inflammatory factors, increased mass, and amplified contractile responses of ASM, and is a critical etiological factor implicated in the dysregulation of ASM function. The molecular pathways through which oxidative stress exerts its effects on ASM in asthma are multifaceted, with the Nrf2/HO-1, MAPK, and PI3K/Akt pathways being particularly noteworthy. These characteristic pathways play a potential role by connecting with different upstream and downstream signaling molecules and are involved in the amplification of ASM inflammatory responses, increased mass, and AHR. This review provides a comprehensive synthesis of the phenotypic expression of ASM dysfunction in asthma, the interplay between oxidants and antioxidants, and the evidence base and molecular underpinnings linking oxidative stress to ASM dysfunction. Given the profound implications of ASM dysfunction on the airflow limitation in asthma and the seminal role of oxidative stress in this process, a deeper exploration of these mechanisms is essential for unraveling the pathogenesis of asthma and may offer novel perspectives for its prophylaxis and management.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Neurological confirmation of periplanone-D exploitation as a primary sex pheromone and counteractions of other components in the smoky brown cockroach Periplaneta fuliginosa.

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-07 DOI: 10.1007/s00441-025-03951-9

Mana Domae, Masazumi Iwasaki, Hiroshi Nishino

引用次数: 0

A brief history of insect neuropeptide and peptide hormone research. 昆虫神经肽和肽激素研究简史。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-01 Epub Date: 2024-12-10 DOI: 10.1007/s00441-024-03936-0

Dick R Nässel

{"title":"A brief history of insect neuropeptide and peptide hormone research.","authors":"Dick R Nässel","doi":"10.1007/s00441-024-03936-0","DOIUrl":"10.1007/s00441-024-03936-0","url":null,"abstract":"This review briefly summarizes 50 years of research on insect neuropeptide and peptide hormone (collectively abbreviated NPH) signaling, starting with the sequencing of proctolin in 1975. The first 25 years, before the sequencing of the Drosophila genome, were characterized by efforts to identify novel NPHs by biochemical means, mapping of their distribution in neurons, neurosecretory cells, and endocrine cells of the intestine. Functional studies of NPHs were predominantly dealing with hormonal aspects of peptides and many employed ex vivo assays. With the annotation of the Drosophila genome, and more specifically of the NPHs and their receptors in Drosophila and other insects, a new era followed. This started with matching of NPH ligands to orphan receptors, and studies to localize NPHs with improved detection methods. Important advances were made with introduction of a rich repertoire of innovative molecular genetic approaches to localize and interfere with expression or function of NPHs and their receptors. These methods enabled cell- or circuit-specific interference with NPH signaling for in vivo assays to determine roles in behavior and physiology, imaging of neuronal activity, and analysis of connectivity in peptidergic circuits. Recent years have seen a dramatic increase in reports on the multiple functions of NPHs in development, physiology and behavior. Importantly, we can now appreciate the pleiotropic functions of NPHs, as well as the functional peptidergic \"networks\" where state dependent NPH signaling ensures behavioral plasticity and systemic homeostasis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"129-159"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles. 基于窦卵泡黄体化细胞的卵巢黄体三维体外模型的建立。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-01 Epub Date: 2024-12-19 DOI: 10.1007/s00441-024-03937-z

Michał M Hryciuk, Filip Schröter, Svenja Claaßen, Christine Aurich, Jella Wauters, Celina Haße, Beate C Braun

{"title":"Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles.","authors":"Michał M Hryciuk, Filip Schröter, Svenja Claaßen, Christine Aurich, Jella Wauters, Celina Haße, Beate C Braun","doi":"10.1007/s00441-024-03937-z","DOIUrl":"10.1007/s00441-024-03937-z","url":null,"abstract":"The study aimed to establish a long-term 3D cell culture model using luteinized follicular cells to investigate the functionality and life cycle of the CL in felids. A mixture of cell types from antral follicles was luteinized in vitro and cultured for up to 23 days. The method, initially applied to the domestic cat, was later extended to Persian and Clouded leopards. Antral follicles were isolated and digested with enzymes; then, the cells were subjected to culture. Experimental subsets were treated with/without 1 µg/mL cloprostenol to validate the cell culture model's suitability for functional studies. In domestic cat samples, microscopic evaluation indicated luteinization, which was confirmed by increased progestagen concentrations and IHC staining for HSD3B and CYP11A1. The gene expression of selected steroidogenic factors (HSD3B1, STAR, CYP11A1) and hormone receptors (LHCGR, PTGFR, PRLR) significantly increased, while CYP17A1 expression decreased. Cloprostenol treatment resulted in reduction of steroidogenic activity, proving its suitability for functional studies. Persian and Clouded leopards' cell cultures exhibited similar patterns in progestagen secretion and gene expression, compared to domestic cats. This model, with its defined luteinization, as well as high and stable progestagen production, allows future investigation of factors regulating CL life cycle and function.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"211-229"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foxa1 disruption enhances human cell integration in human-mouse interspecies chimeras. Foxa1的破坏增强了人-小鼠种间嵌合体中人类细胞的整合。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-01 Epub Date: 2024-12-21 DOI: 10.1007/s00441-024-03941-3

Li-Na Wang, Jun-Shuang Jia, Xing-Long Yang, Yue-Ting Wen, Jing-Xian Liu, Deng-Ke Li, Xing-Rui Chen, Jia-Hong Wang, Ji-Ke Li, Zhong-Xi Huang, Kai-Tai Yao

{"title":"Foxa1 disruption enhances human cell integration in human-mouse interspecies chimeras.","authors":"Li-Na Wang, Jun-Shuang Jia, Xing-Long Yang, Yue-Ting Wen, Jing-Xian Liu, Deng-Ke Li, Xing-Rui Chen, Jia-Hong Wang, Ji-Ke Li, Zhong-Xi Huang, Kai-Tai Yao","doi":"10.1007/s00441-024-03941-3","DOIUrl":"10.1007/s00441-024-03941-3","url":null,"abstract":"Blastocyst complementation can potentially generate a rodent model with humanized nasopharyngeal epithelium (NE) that supports sustained Epstein-Barr virus (EBV) infection, enabling comprehensive studies of EBV biology in nasopharyngeal carcinoma. However, during this process, the specific gene knockouts required to establish a developmental niche for NE remain unclear. We performed bioinformatics analyses and generated Foxa1 mutant mice to confirm that Foxa1 disruption could potentially create a developmental niche for NE. Subsequently, MYD88-inactivated human pluripotent stem cells (hPSCs) were constructed and complemented with Foxa1-deficient mouse blastocysts, with Nosip-deficient mouse blastocysts as a control. The chimerism of human cells in mouse embryos was evaluated from E8.5 to E12.5 using genomic DNA PCR and immunohistochemistry. Our bioinformatics analysis indicated that the expression patterns of Foxa1 in E8.5 to E16.5 mouse embryos underscore its critical role in NE development. The generated mice with Foxa1 disordered region mutations displayed morphological abnormality in NE, suggesting Foxa1-knockouts could potentially establish a developmental niche for NE. In chimeric assays, human cells integrated into 80.00% of Foxa1-deficient embryos, compared with the 4.17% in controls. Immunohistochemistry results revealed robust proliferation of human cells in Foxa1-deficient mouse embryos. However, chimeras from Foxa1-deficient mouse embryos did not survive beyond E10.5, hindering the evaluation of human cell integration in mouse NE. Foxa1 disruption in mouse embryos significantly enhances the integration of human cells in human-mouse interspecies chimeras, thereby facilitating the generation of endoderm-derived organs through blastocyst complementation. Overcoming chimeras' embryonic lethality is crucial for successfully generating humanized NE in Foxa1-deficient mouse embryos.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"231-245"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0