Cell and Tissue Research最新文献_第10页

In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility. 人工睾丸的体外精子发生:目前的知识和男性不育的临床意义。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-09-18 DOI: 10.1007/s00441-023-03824-z

Zahra Bashiri, Mazaher Gholipourmalekabadi, Farnaz Khadivi, Maryam Salem, Azita Afzali, Tat-Chuan Cham, Morteza Koruji

{"title":"In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility.","authors":"Zahra Bashiri, Mazaher Gholipourmalekabadi, Farnaz Khadivi, Maryam Salem, Azita Afzali, Tat-Chuan Cham, Morteza Koruji","doi":"10.1007/s00441-023-03824-z","DOIUrl":"10.1007/s00441-023-03824-z","url":null,"abstract":"Men's reproductive health exclusively depends on the appropriate maturation of certain germ cells known as sperm. Certain illnesses, such as Klinefelter syndrome, cryptorchidism, and syndrome of androgen insensitivity or absence of testis maturation in men, resulting in the loss of germ cells and the removal of essential genes on the Y chromosome, can cause non-obstructive azoospermia. According to laboratory research, preserving, proliferating, differentiating, and transplanting spermatogonial stem cells or testicular tissue could be future methods for preserving the fertility of children with cancer and men with azoospermia. Therefore, new advances in stem cell research may lead to promising therapies for treating male infertility. The rate of progression and breakthrough in the area of in vitro spermatogenesis is lower than that of SSC transplantation, but newer methods are also being developed. In this regard, tissue and cell culture, supplements, and 3D scaffolds have opened new horizons in the differentiation of stem cells in vitro, which could improve the outcomes of male infertility. Various 3D methods have been developed to produce cellular aggregates and mimic the organization and function of the testis. The production of an artificial reproductive organ that supports SSCs differentiation will certainly be a main step in male infertility treatment.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10280933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low acrosin activity is associated with decreased Spam1/acrosin expression and GSH deficiency-caused premature acrosome release of human sperm cells. 顶体蛋白酶活性低与Spam1/顶体蛋白酶表达降低和GSH缺乏导致人类精子细胞顶体过早释放有关。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-10-14 DOI: 10.1007/s00441-023-03826-x

Mengyuan Lin, Pengyun Ling, Qingwen He, Daozhen Chen, Lianshuai Zheng, Lisha Tang, Shi-Wen Jiang

{"title":"Low acrosin activity is associated with decreased Spam1/acrosin expression and GSH deficiency-caused premature acrosome release of human sperm cells.","authors":"Mengyuan Lin, Pengyun Ling, Qingwen He, Daozhen Chen, Lianshuai Zheng, Lisha Tang, Shi-Wen Jiang","doi":"10.1007/s00441-023-03826-x","DOIUrl":"10.1007/s00441-023-03826-x","url":null,"abstract":"Low acrosin activity (LAA) is associated with sperm function anomaly and poor outcomes of in vitro fertilization. In this study, we confirm that 993 semen samples with LAA had a reduced sperm motility and low in vitro fertilization rate in comparison with 1332 normal controls (NC). Proteomic comparison between 11 LAA and 11 NC sperm samples identified 35 upregulated and 99 downregulated proteins in the LAA group. Indeed, proteomic data showed that acrosome enzymes Spam1 and Acrosin were among the downregulated proteins in the LAA group, which was validated by quantitative PCR and immunefluorescent staining of sperm cells. The KEEG pathway analysis revealed a deficiency of GSH and Gln biosynthesis in LAA sperm cells. Immunofluorescent staining of sperms and quantitative PCR verified downregulation of GLUL and GCLC, the key enzymes for GSH and Gln biosynthesis. Moreover, the results of ELISA assay confirmed low levels of GSH and Gln in LAA sperm cells. Mechanistic studies showed that addition of 10 mM H2O2 to semen samples led to a significant reduction of acrosin activity and sperm motility, most possibly by triggering premature acrosome release. In contrast, the presence of 20 mM GSH blocked the oxidative effects of H2O2. Since GSH counteracts the oxidative stress and Gln participates in TCA cycling, their deficiency may affect the redox balance as well as energy production of sperm cells. These findings shed new light on the pathological mechanisms of infertility associated with LAA. Male infertility patients could benefit from GSH supplement by improvement of acrosin activity and other sperm functions.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41192564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CDKN2B-AS1 mediates proliferation and migration of vascular smooth muscle cells induced by insulin. CDKN2B-AS1介导胰岛素诱导的血管平滑肌细胞的增殖和迁移。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-11-01 DOI: 10.1007/s00441-023-03836-9

Hao-Jie Jin, Zi-Heng Wu, Bao-Fu Zhang, Jie Deng, Yin-Dong Xu, Xin-Yu Wang, Zheng-Yang Song, Xin-Wu Lu, Wan-Tie Wang, Xiang-Tao Zheng

{"title":"CDKN2B-AS1 mediates proliferation and migration of vascular smooth muscle cells induced by insulin.","authors":"Hao-Jie Jin, Zi-Heng Wu, Bao-Fu Zhang, Jie Deng, Yin-Dong Xu, Xin-Yu Wang, Zheng-Yang Song, Xin-Wu Lu, Wan-Tie Wang, Xiang-Tao Zheng","doi":"10.1007/s00441-023-03836-9","DOIUrl":"10.1007/s00441-023-03836-9","url":null,"abstract":"Excessive proliferation and migration of vascular smooth muscle cells (VSMCs) contribute to the intimal hyperplasia in type 2 diabetes mellitus (T2DM) patients after percutaneous coronary intervention. We aimed to investigate the role of lncRNA cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1) in VSMC proliferation and migration, as well as the underlying mechanism. T2DM model mice with carotid balloon injury were used in vivo and mouse aortic vascular smooth muscle cells (MOVAS) stimulated by insulin were used in vitro to assess the role of CDKN2B-AS1 in VSMC proliferation and migration following vascular injury in T2DM state. To investigate cell viability and migration, MTT assay and Transwell assay were conducted. To elucidate the underlying molecular mechanisms, the methylation-specific polymerase chain reaction, RNA immunoprecipitation, RNA-pull down, co-immunoprecipitation, and chromatin immunoprecipitation were performed. In vivo, CDKN2B-AS1 was up-regulated in common carotid artery tissues. In vitro, insulin treatment increased CDKN2B-AS1 level, enhanced MOVAS cell proliferation and migration, while the promoting effect was reversed by CDKN2B-AS1 knockdown. CDKN2B-AS1 forms a complex with enhancer of zeste homolog 2 (EZH2) and DNA methyltransferase (cytosine-5) 1 (DNMT1) to regulate smooth muscle 22 alpha (SM22α) methylation levels. In insulin-stimulated cells, SM22α knockdown abrogated the inhibitory effect of CDKN2B-AS1 knockdown on cell viability and migration. Injection of lentivirus-sh-CDKN2B-AS1 relieved intimal hyperplasia in T2DM mice with carotid balloon injury. Up-regulation of CDKN2B-AS1 induced by insulin promotes cell proliferation and migration by targeting SM22α through forming a complex with EZH2 and DNMT1, thereby aggravating the intimal hyperplasia after vascular injury in T2DM.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71421049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs in atrial fibrillation target genes in structural remodelling. 心房颤动中的微小RNA靶向结构重塑的基因。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-10-14 DOI: 10.1007/s00441-023-03823-0

Nicoline W E van den Berg, Makiri Kawasaki, Fransisca A Nariswari, Benedetta Fabrizi, Jolien Neefs, Ingeborg van der Made, Robin Wesselink, Wim Jan P van Boven, Antoine H G Driessen, Aldo Jongejan, Joris R de Groot

{"title":"MicroRNAs in atrial fibrillation target genes in structural remodelling.","authors":"Nicoline W E van den Berg, Makiri Kawasaki, Fransisca A Nariswari, Benedetta Fabrizi, Jolien Neefs, Ingeborg van der Made, Robin Wesselink, Wim Jan P van Boven, Antoine H G Driessen, Aldo Jongejan, Joris R de Groot","doi":"10.1007/s00441-023-03823-0","DOIUrl":"10.1007/s00441-023-03823-0","url":null,"abstract":"We aim to elucidate how miRNAs regulate the mRNA signature of atrial fibrillation (AF), to gain mechanistic insight and identify candidate targets for future therapies. We present combined miRNA-mRNA sequencing using atrial tissues of patient without AF (n = 22), with paroxysmal AF (n = 22) and with persistent AF (n = 20). mRNA sequencing previously uncovered upregulated epithelial to mesenchymal transition, endothelial cell proliferation and extracellular matrix remodelling involving glycoproteins and proteoglycans in AF. MiRNA co-sequencing discovered miRNAs regulating the mRNA expression changes. Key downregulated miRNAs included miR-135b-5p, miR-138-5p, miR-200a-3p, miR-200b-3p and miR-31-5p and key upregulated miRNAs were miR-144-3p, miR-15b-3p, miR-182-5p miR-18b-5p, miR-4306 and miR-206. MiRNA expression levels were negatively correlated with the expression levels of a multitude of predicted target genes. Downregulated miRNAs associated with increased gene expression are involved in upregulated epithelial and endothelial cell migration and glycosaminoglycan biosynthesis. In vitro inhibition of miR-135b-5p and miR-138-5p validated an effect of miRNAs on multiple predicted targets. Altogether, the discovered miRNAs may be explored in further functional studies as potential targets for anti-fibrotic therapies in AF.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10697892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41192565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and evolution of serotonergic cells in taste buds of elasmobranchs and ancestral actinopterygian fish. 蓝鳃类和祖先放线鳍鱼类味蕾中5-羟色胺能细胞的多样性和进化。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1007/s00441-023-03837-8

Takanori Ikenaga, Tastufumi Nakamura, Tatsushi Tajiri, Minaki Tsuji, Dai-Ichiro Kato, Toshinao Ineno, Yasuhisa Kobayashi, Naoaki Tsutsui, Sadao Kiyohara

{"title":"Diversity and evolution of serotonergic cells in taste buds of elasmobranchs and ancestral actinopterygian fish.","authors":"Takanori Ikenaga, Tastufumi Nakamura, Tatsushi Tajiri, Minaki Tsuji, Dai-Ichiro Kato, Toshinao Ineno, Yasuhisa Kobayashi, Naoaki Tsutsui, Sadao Kiyohara","doi":"10.1007/s00441-023-03837-8","DOIUrl":"10.1007/s00441-023-03837-8","url":null,"abstract":"A subset of gustatory cells are serotonin immunoreactive (ir) in the mammalian taste bud. In the taste bud of lamprey, elongated gustatory-like cells are also serotonin-ir. In contrast, flattened serotonin-ir cells are located only in the basal region of the taste buds in the teleosts and amphibians. These serotonin-ir cells are termed as basal cells. To evaluate the evolution and diversity of serotonergic cells in the taste bud of amniote animals, we explored the distribution and morphology of serotonin-ir cells in the taste buds of ancestral actinopterygian fish (spotted gar, sturgeon, Polypterus senegalus) and elasmobranch (stingray). In all examined animals, the taste buds contained serotonin-ir cells in their basal part. The number of serotonin-ir basal cells in each taste bud was different between these fish species. They were highest in the stingray and decreased in the order of the Polypterus, sturgeon, and gar. While serotonin immunoreactivity was observed only in the basal cells in the taste buds of the ancestral actinopterygian fish, some elongated cells were also serotonin-ir in addition to the basal cells in the stingray taste buds. mRNA of tryptophan hydroxylase 1 (tph1), a rate-limiting enzyme of the serotonin synthesis, is expressed in both the elongated and basal cells of stingray taste buds, indicating that these cells synthesize the serotonin by themselves. These results suggest that the serotonin-ir basal cells arose from the ancestor of the cartilaginous fish, and serotonin-ir cells in the elasmobranch taste bud exhibit an intermediate aspect between the lamprey and actinopterygian fish.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long non-coding RNA HOX transcript antisense intergenic RNA depletion protects against alcoholic hepatitis through the microRNA-148a-3p/sphingosine 1-phosphate receptor 1 axis. 长非编码RNA HOX转录物反义基因间RNA缺失通过微小RNA-148a-3p/鞘氨醇1-磷酸受体1轴保护免受酒精性肝炎的侵袭。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1007/s00441-023-03835-w

Dan Chen, Ping Lu, Tianfeng Sun, Aliang Ding

{"title":"Long non-coding RNA HOX transcript antisense intergenic RNA depletion protects against alcoholic hepatitis through the microRNA-148a-3p/sphingosine 1-phosphate receptor 1 axis.","authors":"Dan Chen, Ping Lu, Tianfeng Sun, Aliang Ding","doi":"10.1007/s00441-023-03835-w","DOIUrl":"10.1007/s00441-023-03835-w","url":null,"abstract":"The aggravating role of long noncoding RNA (lncRNA) HOTAIR has been indicated in liver injury caused by hepatic ischemia/reperfusion. However, under the condition of alcoholic hepatitis (AH), its effects remain unclear. The present study aimed to examine the effect of lncRNA HOTAIR on hepatic stellate cell viability and apoptosis during liver injury caused by AH. In the liver tissues of AH rats, HOTAIR and S1PR1 were overexpressed, and microRNA (miR)-148a-3p was poorly expressed. Loss-of-function assays revealed that silencing of HOTAIR alleviated liver injury in AH by inhibiting the activated phenotype of hepatic stellate cells, inflammation, and fibrosis. Using the bioinformatics databases, dual-luciferase, RIP, and FISH assays, we observed that HOTAIR was mainly localized in the cytoplasm of hepatic stellate cells, and HOTAIR could bind specifically to miR-148a-3p. In addition, miR-148a-3p could target S1PR1 expression. Rescue experiments showed that silencing of miR-148a-3p or overexpression of S1PR1 reversed the alleviating effects of HOTAIR silencing on liver injury. Taken together, our findings revealed that HOTAIR regulates hepatic stellate cell proliferation via the miR-148a-3p/S1PR1 axis in liver injury, which may serve as the basis for developing novel therapeutic strategies to treat AH.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The function of exosomes in ovarian granulosa cells. 卵巢颗粒细胞外泌体的功能。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-08-21 DOI: 10.1007/s00441-023-03820-3

Ruotong Zhou, Dan Liu

引用次数: 0

The fate of adipose tissue and adipose-derived stem cells in allograft. 异体移植中脂肪组织和脂肪干细胞的命运。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-08-25 DOI: 10.1007/s00441-023-03827-w

Sadia Farhana, Yew Chun Kai, Ramlah Kadir, Wan Azman Wan Sulaiman, Nor Asyikin Nordin, Nur Azida Mohd Nasir

{"title":"The fate of adipose tissue and adipose-derived stem cells in allograft.","authors":"Sadia Farhana, Yew Chun Kai, Ramlah Kadir, Wan Azman Wan Sulaiman, Nor Asyikin Nordin, Nur Azida Mohd Nasir","doi":"10.1007/s00441-023-03827-w","DOIUrl":"10.1007/s00441-023-03827-w","url":null,"abstract":"Utilizing adipose tissue and adipose-derived stem cells (ADSCs) turned into a promising field of allograft in recent years. The therapeutic potential of adipose tissue and ADSCs is governed by their molecular secretions, ability to sustain multi-differentiation and self-renewal which are pivotal in reconstructive, genetic diseases, and cosmetic goals. However, revisiting the existing functional capacity of adipose tissue and ADSCs and their intricate relationship with allograft is crucial to figure out the remarkable question of safety to use in allograft due to the growing evidence of interactions between tumor microenvironment and ADSCs. For instance, the molecular secretions of adipose tissue and ADSCs induce angiogenesis, create growth factors, and control the inflammatory response; it has now been well determined. Though the existing preclinical allograft studies gave positive feedback, ADSCs and adipose tissue are attracted by some factors of tumor stroma. Moreover, allorecognition is pivotal to allograft rejection which is carried out by costimulation in a complement-dependent way and leads to the destruction of the donor cells. However, extensive preclinical trials of adipose tissue and ADSCs in allograft at molecular level are still limited. Hence, comprehensive immunomodulatory analysis could ensure the successful allograft of adipose tissue and ADSCs avoiding the oncological risk.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10124615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of eMSCs with HA-GEL system in repairing damaged endometrium after endometrial cancer with fertility-sparing treatment. 应用HA-GEL系统的eMSCs修复癌症子宫内膜异位治疗后受损子宫内膜的探讨。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-09-28 DOI: 10.1007/s00441-023-03831-0

Wei Liu, Mengxin Hao, Yuhui Xu, Xiaojun Ren, Jiali Hu, Lulu Wang, Xiaojun Chen, Qiaoying Lv

{"title":"Exploration of eMSCs with HA-GEL system in repairing damaged endometrium after endometrial cancer with fertility-sparing treatment.","authors":"Wei Liu, Mengxin Hao, Yuhui Xu, Xiaojun Ren, Jiali Hu, Lulu Wang, Xiaojun Chen, Qiaoying Lv","doi":"10.1007/s00441-023-03831-0","DOIUrl":"10.1007/s00441-023-03831-0","url":null,"abstract":"Despite the high complete response rate of fertility-sparing treatment in early-stage endometrial cancer (EC), the low pregnancy rate is a clinical challenge. Whether endometrium-derived mesenchymal stem cells (eMSCs) can repair damaged endometrium after EC reversal remains unclear. This study explored the potential therapeutic effects of eMSCs with suitable scaffold materials on endometrial damage caused by EC. Here, appropriate engineering scaffold materials were compared to identify the most suitable materials to carry eMSCs. Then, safety and efficacy evaluations of eMSCs with a suitable hyaluronic acid hydrogel (eMSCs/HA-GEL) were investigated in in vivo experiments with subcutaneous xenotransplantation in Balb/C nude mice and a model of endometrial mechanical injury in rats. HA-GEL has minimal cytotoxicity to eMSCs compared to other materials. Then, in vitro experiments demonstrate that eMSCs/HA-GEL enhance the inhibitory effects of progestins on EC cell biological behaviors. eMSCs/HA-GEL significantly inhibit EC cell growth and have no potential safety hazards of spontaneous tumorigenesis in Balb/C nude mouse subcutaneous xenotransplantation assays. eMSCs/HA-GEL intrauterine transplantation effectively increases endometrial thickness and glandular number, improves endometrial blood supply, reduces fibrotic areas, and improves pregnancy rates in a rat endometrial mechanical injury model. GFP-eMSCs/HA-GEL intrauterine transplantation in rats shows more GFP-eMSCs in the endometrium than GFP-eMSCs transplantation alone, and no tumor formation or suspicious cell nodules are found in the liver, kidney, or lung tissues. Our results reveal the safety and efficacy of eMSCs/HA-GEL in animal models and provide preliminary evidence for the use of eMSCs/HA-GEL as a treatment for EC-related endometrial damage.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41100491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regeneration of starfish radial nerve cord restores animal mobility and unveils a new coelomocyte population. 海星桡神经索的再生恢复了动物的活动能力，揭示了一个新的腔胚细胞群。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-11-01 Epub Date: 2023-08-22 DOI: 10.1007/s00441-023-03818-x

Filipe Magalhães, Claúdia Andrade, Beatriz Simões, Fredi Brigham, Ruben Valente, Pedro Martinez, José Rino, Michela Sugni, Ana Varela Coelho

{"title":"Regeneration of starfish radial nerve cord restores animal mobility and unveils a new coelomocyte population.","authors":"Filipe Magalhães, Claúdia Andrade, Beatriz Simões, Fredi Brigham, Ruben Valente, Pedro Martinez, José Rino, Michela Sugni, Ana Varela Coelho","doi":"10.1007/s00441-023-03818-x","DOIUrl":"10.1007/s00441-023-03818-x","url":null,"abstract":"The potential to regenerate a damaged body part is expressed to a different extent in animals. Echinoderms, in particular starfish, are known for their outstanding regenerating potential. Differently, humans have restricted abilities to restore organ systems being dependent on limited sources of stem cells. In particular, the potential to regenerate the central nervous system is extremely limited, explaining the lack of natural mechanisms that could overcome the development of neurodegenerative diseases and the occurrence of trauma. Therefore, understanding the molecular and cellular mechanisms of regeneration in starfish could help the development of new therapeutic approaches in humans. In this study, we tackle the problem of starfish central nervous system regeneration by examining the external and internal anatomical and behavioral traits, the dynamics of coelomocyte populations, and neuronal tissue architecture after radial nerve cord (RNC) partial ablation. We noticed that the removal of part of RNC generated several anatomic anomalies and induced behavioral modifications (injured arm could not be used anymore to lead the starfish movement). Those alterations seem to be related to defense mechanisms and protection of the wound. In particular, histology showed that tissue patterns during regeneration resemble those described in holothurians and in starfish arm tip regeneration. Flow cytometry coupled with imaging flow cytometry unveiled a new coelomocyte population during the late phase of the regeneration process. Morphotypes of these and previously characterized coelomocyte populations were described based on IFC data. Further studies of this new coelomocyte population might provide insights on their involvement in radial nerve cord regeneration.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10040545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2