Cell and Tissue Research最新文献_第10页

Current methods for the microglia isolation: Overview and comparative analysis of approaches 目前分离小胶质细胞的方法：方法概述与比较分析

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-15 DOI: 10.1007/s00441-023-03853-8

E. R. Akhmetzyanova, A. A. Rizvanov, Y. O. Mukhamedshina

{"title":"Current methods for the microglia isolation: Overview and comparative analysis of approaches","authors":"E. R. Akhmetzyanova, A. A. Rizvanov, Y. O. Mukhamedshina","doi":"10.1007/s00441-023-03853-8","DOIUrl":"https://doi.org/10.1007/s00441-023-03853-8","url":null,"abstract":"Microglia represent a distinct population of neuroglia, constituting ~ 10% of all CNS cells and exhibit high plasticity. Proper functioning of microglia is critical in the event of CNS damage due to the rapid modulation of their functions. Microglia are not only the first stage of immune defense against injury and infection, contributing to both the innate and adaptive local immune response, but also play a vital role in maintaining homeostasis of the brain and spinal cord. For this reason, microglia deserve special attention in the study of neuropathological responses. Studying microglia behavior in various in vivo models of neuropathologies is certainly a priority, as it allows us to evaluate the behavior in the context of the changing microenvironment of nervous tissue. However, sometimes there are some technological problems that hinder the identification of the features of intercellular interactions, ensured cooperation between microglia and other cell types. In this regard, the use of in vitro models remains relevant today, contributing to a more in-depth understanding of the mechanisms of microglial involvement in neuropathology. The methods considered in this review for obtaining an isolated culture of microglia, along with their advantages and disadvantages, can help researchers in selecting the appropriate source and method for obtaining these cells, thereby opening up opportunities for gaining new neurobiological knowledge.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"46 1 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138691121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adropin, a novel hepatokine: localization and expression during postnatal development and its impact on testicular functions of pre-pubertal mice 一种新型肝素--Adropin：产后发育过程中的定位和表达及其对青春期前小鼠睾丸功能的影响

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-13 DOI: 10.1007/s00441-023-03852-9

Shashank Tripathi, Shweta Maurya, Ajit Singh

{"title":"Adropin, a novel hepatokine: localization and expression during postnatal development and its impact on testicular functions of pre-pubertal mice","authors":"Shashank Tripathi, Shweta Maurya, Ajit Singh","doi":"10.1007/s00441-023-03852-9","DOIUrl":"https://doi.org/10.1007/s00441-023-03852-9","url":null,"abstract":"Adropin, a multifaceted peptide, was identified as a new metabolic hormone responsible for regulating gluco-lipid homeostasis. However, its role in the testicular function is not yet understood. We aimed to investigate the localization and expression of adropin and GPR19 during different phases of postnatal development. Immunohistochemical study revealed the intense reactivity of adropin in the Leydig cells during all phases of postnatal development, while GPR19 showed intense immunoreactivity in the pachytene spermatocytes and mild immunoreactivity in Leydig cells as well as primary and secondary spermatocytes. Western blot study revealed maximum expression of GPR19 in pre-pubertal mouse testis that clearly indicates maximum responsiveness of adropin during that period. So, we hypothesized that adropin may act as an autocrine/paracrine factor that regulates pubertal changes in mouse testis. To examine the effect of adropin on pubertal onset, we gave bilateral intra-testicular doses (0.5 and 1.5 µg/testis) to pre-pubertal mice. Adropin treatment promoted testicular testosterone synthesis by increasing the expression of StAR, 3β-HSD, and 17β-HSD. Adropin also promoted germ cell survival and proliferation by upregulating the expression of PCNA and downregulating the Bax/Bcl2 ratio and Caspase 3 expression resulting in fewer TUNEL-positive cells in adropin-treated groups. FACS analysis demonstrated that adropin treatment not only increases 1C to 4C ratio but also significantly increases the 1C (spermatid) and 1C to 2C ratio which demarcates accelerated germ cell differentiation and turnover of testicular cells. In conclusion, adropin promotes steroidogenesis, germ cell survival, as well as the proliferation in the pre-pubertal mouse testis that may hasten the pubertal transition in an autocrine/paracrine manner.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"31 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138632542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular heterogeneity in red and melanized focal muscle changes in farmed Atlantic salmon (Salmo salar) visualized by spatial transcriptomics 通过空间转录组学观察养殖大西洋鲑（Salmo salar）红色和黑色病灶肌肉变化的细胞异质性

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-13 DOI: 10.1007/s00441-023-03850-x

H. M. Bjørgen, S. Malik, E. Rimstad, M. Vaadal, I. B. Nyman, E. O. Koppang, T. Tengs

引用次数: 0

ALDH1A1 and ALDH1A3 paralogues of aldehyde dehydrogenase 1 control myogenic differentiation of skeletal muscle satellite cells by retinoic acid-dependent and -independent mechanisms. 醛脱氢酶1的ALDH1A1和ALDH1A3同源物通过维甲酸依赖性和非依赖性机制控制骨骼肌卫星细胞的肌源性分化。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-10-31 DOI: 10.1007/s00441-023-03838-7

Laura Steingruber, Florian Krabichler, Sophie Franzmeier, Wei Wu, Jürgen Schlegel, Marco Koch

{"title":"ALDH1A1 and ALDH1A3 paralogues of aldehyde dehydrogenase 1 control myogenic differentiation of skeletal muscle satellite cells by retinoic acid-dependent and -independent mechanisms.","authors":"Laura Steingruber, Florian Krabichler, Sophie Franzmeier, Wei Wu, Jürgen Schlegel, Marco Koch","doi":"10.1007/s00441-023-03838-7","DOIUrl":"10.1007/s00441-023-03838-7","url":null,"abstract":"ALDH1A1 and ALDH1A3 paralogues of aldehyde dehydrogenase 1 (ALDH1) control myogenic differentiation of skeletal muscle satellite cells (SC) by formation of retinoic acid (RA) and subsequent cell cycle adjustments. The respective relevance of each paralogue for myogenic differentiation and the mechanistic interaction of each paralogue within RA-dependent and RA-independent pathways remain elusive.We analysed the impact of ALDH1A1 and ALDH1A3 activity on myogenesis of murine C2C12 myoblasts. Both paralogues are pivotal factors in myogenic differentiation, since CRISPR/Cas9-edited single paralogue knock-out impaired serum withdrawal-induced myogenic differentiation, while successive recombinant re-expression of ALDH1A1 or ALDH1A3, respectively, in the corresponding ALDH1 paralogue single knock-out cell lines, recovered the differentiation potential. Loss of differentiation in single knock-out cell lines was restored by treatment with RA-analogue TTNPB, while RA-receptor antagonization by AGN 193109 inhibited differentiation of wildtype cell lines, supporting the idea that RA-dependent pathway is pivotal for myogenic differentiation which is accomplished by both paralogues.However, overexpression of ALDH1-paralogues or disulfiram-mediated inhibition of ALDH1 enzymatic activity not only increased ALDH1A1 and ALDH1A3 protein levels but also induced subsequent differentiation of C2C12 myoblasts independently from serum withdrawal, indicating that ALDH1-dependent myogenic differentiation relies on different cellular conditions. Remarkably, ALDH1-paralogue knock-out impaired the autophagic flux, namely autophagosome cargo protein p62 formation and LC3B-I to LC3B-II conversion, demonstrating that ALDH1-paralogues interact with autophagy in myogenesis. Together, ALDH1 paralogues play a crucial role in myogenesis by orchestration of complex RA-dependent and RA-independent pathways.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"515-528"},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-regional expression of pancreas-related digestive enzyme genes in the intestinal chamber of the ascidian Ciona intestinalis type A. A型海鞘肠腔胰腺相关消化酶基因的多区域表达

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-10-25 DOI: 10.1007/s00441-023-03839-6

Rin Iguchi, Kanae Usui, Satoshi Nakayama, Yasunori Sasakura, Toshio Sekiguchi, Michio Ogasawara

{"title":"Multi-regional expression of pancreas-related digestive enzyme genes in the intestinal chamber of the ascidian Ciona intestinalis type A.","authors":"Rin Iguchi, Kanae Usui, Satoshi Nakayama, Yasunori Sasakura, Toshio Sekiguchi, Michio Ogasawara","doi":"10.1007/s00441-023-03839-6","DOIUrl":"10.1007/s00441-023-03839-6","url":null,"abstract":"Bilateria share sequential steps in their digestive systems, and digestion occurs in a pre-absorption step within a chamber-like structure. Previous studies on the ascidian Ciona intestinalis type A, an evolutionary research model of vertebrate organs, revealed that Ciona homologs of pancreas-related exocrine digestive enzymes (XDEs) are exclusively expressed in the chamber-like bulging stomach. In the development of the gastrointestinal tract, genes for the pancreas-related transcription factors, namely Ptf1a, Nr5a2, and Pdx, are expressed near the stomach. Recent organ/tissue RNA-seq studies on two Ciona species reported that transcripts of the XDE homologs exist in the intestinal regions, as well as in the stomach. In the present study, we investigated the spatial gene expression of XDE homologs in the gastrointestinal region of the C. intestinalis type A. Whole-mount in situ hybridization using adult and juvenile specimens revealed apparent expression signals of XDE homologs in a small number of gastrointestinal epithelial cells. Furthermore, two pancreas-related transcription factor genes, Nr5a2 and Pdx, exhibited multi-regional expression along the Ciona juvenile intestines. These results imply that ascidians may form multiple digestive regions corresponding to the vertebrate pancreas.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"423-430"},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50157139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Missing pieces of the pituitary puzzle: participation of extra-adenohypophyseal placode-lineage cells in the adult pituitary gland. 垂体之谜的缺失部分:腺垂体外基板谱系细胞在成人垂体中的参与。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-08-31 DOI: 10.1007/s00441-023-03829-8

Yukio Kato, Saishu Yoshida, Takako Kato

引用次数: 0

lncRNA SNHG9 enhances liver cancer stem cell self-renewal and tumorigenicity by negatively regulating PTEN expression via recruiting EZH2. lncRNA SNHG9通过募集EZH2负调控PTEN表达，增强癌症干细胞的自我更新和致瘤性。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1007/s00441-023-03834-x

Shouzhang Yang, Xiaojiao Ruan, Bingren Hu, Jinfu Tu, Huajie Cai

{"title":"lncRNA SNHG9 enhances liver cancer stem cell self-renewal and tumorigenicity by negatively regulating PTEN expression via recruiting EZH2.","authors":"Shouzhang Yang, Xiaojiao Ruan, Bingren Hu, Jinfu Tu, Huajie Cai","doi":"10.1007/s00441-023-03834-x","DOIUrl":"10.1007/s00441-023-03834-x","url":null,"abstract":"Liver cancer stem cell (CSC) self-renewal and tumorigenesis are important causes of hepatocellular carcinoma (HCC) recurrence. We purposed to investigate the function of long noncoding RNA small nucleolar RNA host gene 9 (SNHG9) in liver CSC self-renewal and tumorigenesis in this study. Flow cytometry was carried out to separate CD133+ Populations and CD133- Populations from HCC cell lines. A combination of CD133+ cells and Matrigel matrix was subcutaneously injected to create the NOD-SCID mouse xenograft tumor model. Colony formation test and spheroids formation assay were carried out to clarify the impact of SNHG9 on the self-renewal of liver CSCs. RNA immunoprecipitation, RNA-pull down, and chromatin immunoprecipitation were performed on CD133+ cells to elucidate the mechanism of SNHG9 regulating PTEN expression. We found that SNHG9 was highly expressed in HCC clinical samples, HCC cells, and CD133+ cells. In vitro, interference with SNHG9 prevented the formation of colonies and spheroids in liver CSC cells and primary HCC cells. In vivo, interference with SNHG9 reduced the tumor volume and weight. SNHG9 could bind to EZH2, and SNHG9 interference suppressed EZH2 recruitment and H3K27me3 levels in the PTEN promoter region. In addition, SNHG9 inhibition promoted PTEN expression while having little impact on EZH2 levels. Interference with SNHG9 inhibited liver CSC self-renewal and tumorigenesis by up-regulating PTEN levels. In conclusion, by binding to EZH2, SNHG9 down-regulated PTEN levels, promoting liver CSC self-renewal and tumor formation, and exacerbating HCC progression.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"441-453"},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility. 人工睾丸的体外精子发生:目前的知识和男性不育的临床意义。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-09-18 DOI: 10.1007/s00441-023-03824-z

Zahra Bashiri, Mazaher Gholipourmalekabadi, Farnaz Khadivi, Maryam Salem, Azita Afzali, Tat-Chuan Cham, Morteza Koruji

{"title":"In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility.","authors":"Zahra Bashiri, Mazaher Gholipourmalekabadi, Farnaz Khadivi, Maryam Salem, Azita Afzali, Tat-Chuan Cham, Morteza Koruji","doi":"10.1007/s00441-023-03824-z","DOIUrl":"10.1007/s00441-023-03824-z","url":null,"abstract":"Men's reproductive health exclusively depends on the appropriate maturation of certain germ cells known as sperm. Certain illnesses, such as Klinefelter syndrome, cryptorchidism, and syndrome of androgen insensitivity or absence of testis maturation in men, resulting in the loss of germ cells and the removal of essential genes on the Y chromosome, can cause non-obstructive azoospermia. According to laboratory research, preserving, proliferating, differentiating, and transplanting spermatogonial stem cells or testicular tissue could be future methods for preserving the fertility of children with cancer and men with azoospermia. Therefore, new advances in stem cell research may lead to promising therapies for treating male infertility. The rate of progression and breakthrough in the area of in vitro spermatogenesis is lower than that of SSC transplantation, but newer methods are also being developed. In this regard, tissue and cell culture, supplements, and 3D scaffolds have opened new horizons in the differentiation of stem cells in vitro, which could improve the outcomes of male infertility. Various 3D methods have been developed to produce cellular aggregates and mimic the organization and function of the testis. The production of an artificial reproductive organ that supports SSCs differentiation will certainly be a main step in male infertility treatment.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"393-421"},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10280933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low acrosin activity is associated with decreased Spam1/acrosin expression and GSH deficiency-caused premature acrosome release of human sperm cells. 顶体蛋白酶活性低与Spam1/顶体蛋白酶表达降低和GSH缺乏导致人类精子细胞顶体过早释放有关。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-10-14 DOI: 10.1007/s00441-023-03826-x

Mengyuan Lin, Pengyun Ling, Qingwen He, Daozhen Chen, Lianshuai Zheng, Lisha Tang, Shi-Wen Jiang

{"title":"Low acrosin activity is associated with decreased Spam1/acrosin expression and GSH deficiency-caused premature acrosome release of human sperm cells.","authors":"Mengyuan Lin, Pengyun Ling, Qingwen He, Daozhen Chen, Lianshuai Zheng, Lisha Tang, Shi-Wen Jiang","doi":"10.1007/s00441-023-03826-x","DOIUrl":"10.1007/s00441-023-03826-x","url":null,"abstract":"Low acrosin activity (LAA) is associated with sperm function anomaly and poor outcomes of in vitro fertilization. In this study, we confirm that 993 semen samples with LAA had a reduced sperm motility and low in vitro fertilization rate in comparison with 1332 normal controls (NC). Proteomic comparison between 11 LAA and 11 NC sperm samples identified 35 upregulated and 99 downregulated proteins in the LAA group. Indeed, proteomic data showed that acrosome enzymes Spam1 and Acrosin were among the downregulated proteins in the LAA group, which was validated by quantitative PCR and immunefluorescent staining of sperm cells. The KEEG pathway analysis revealed a deficiency of GSH and Gln biosynthesis in LAA sperm cells. Immunofluorescent staining of sperms and quantitative PCR verified downregulation of GLUL and GCLC, the key enzymes for GSH and Gln biosynthesis. Moreover, the results of ELISA assay confirmed low levels of GSH and Gln in LAA sperm cells. Mechanistic studies showed that addition of 10 mM H2O2 to semen samples led to a significant reduction of acrosin activity and sperm motility, most possibly by triggering premature acrosome release. In contrast, the presence of 20 mM GSH blocked the oxidative effects of H2O2. Since GSH counteracts the oxidative stress and Gln participates in TCA cycling, their deficiency may affect the redox balance as well as energy production of sperm cells. These findings shed new light on the pathological mechanisms of infertility associated with LAA. Male infertility patients could benefit from GSH supplement by improvement of acrosin activity and other sperm functions.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"529-545"},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41192564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CDKN2B-AS1 mediates proliferation and migration of vascular smooth muscle cells induced by insulin. CDKN2B-AS1介导胰岛素诱导的血管平滑肌细胞的增殖和迁移。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-01 Epub Date: 2023-11-01 DOI: 10.1007/s00441-023-03836-9

Hao-Jie Jin, Zi-Heng Wu, Bao-Fu Zhang, Jie Deng, Yin-Dong Xu, Xin-Yu Wang, Zheng-Yang Song, Xin-Wu Lu, Wan-Tie Wang, Xiang-Tao Zheng

{"title":"CDKN2B-AS1 mediates proliferation and migration of vascular smooth muscle cells induced by insulin.","authors":"Hao-Jie Jin, Zi-Heng Wu, Bao-Fu Zhang, Jie Deng, Yin-Dong Xu, Xin-Yu Wang, Zheng-Yang Song, Xin-Wu Lu, Wan-Tie Wang, Xiang-Tao Zheng","doi":"10.1007/s00441-023-03836-9","DOIUrl":"10.1007/s00441-023-03836-9","url":null,"abstract":"Excessive proliferation and migration of vascular smooth muscle cells (VSMCs) contribute to the intimal hyperplasia in type 2 diabetes mellitus (T2DM) patients after percutaneous coronary intervention. We aimed to investigate the role of lncRNA cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1) in VSMC proliferation and migration, as well as the underlying mechanism. T2DM model mice with carotid balloon injury were used in vivo and mouse aortic vascular smooth muscle cells (MOVAS) stimulated by insulin were used in vitro to assess the role of CDKN2B-AS1 in VSMC proliferation and migration following vascular injury in T2DM state. To investigate cell viability and migration, MTT assay and Transwell assay were conducted. To elucidate the underlying molecular mechanisms, the methylation-specific polymerase chain reaction, RNA immunoprecipitation, RNA-pull down, co-immunoprecipitation, and chromatin immunoprecipitation were performed. In vivo, CDKN2B-AS1 was up-regulated in common carotid artery tissues. In vitro, insulin treatment increased CDKN2B-AS1 level, enhanced MOVAS cell proliferation and migration, while the promoting effect was reversed by CDKN2B-AS1 knockdown. CDKN2B-AS1 forms a complex with enhancer of zeste homolog 2 (EZH2) and DNA methyltransferase (cytosine-5) 1 (DNMT1) to regulate smooth muscle 22 alpha (SM22α) methylation levels. In insulin-stimulated cells, SM22α knockdown abrogated the inhibitory effect of CDKN2B-AS1 knockdown on cell viability and migration. Injection of lentivirus-sh-CDKN2B-AS1 relieved intimal hyperplasia in T2DM mice with carotid balloon injury. Up-regulation of CDKN2B-AS1 induced by insulin promotes cell proliferation and migration by targeting SM22α through forming a complex with EZH2 and DNMT1, thereby aggravating the intimal hyperplasia after vascular injury in T2DM.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"455-469"},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71421049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0