Cell and Tissue Research最新文献

{"title":"Role of capillary rarefaction in age-related changes of external urethral sphincter muscle of female mice.","authors":"Retsu Mitsui, Kyoko Miwa-Nishimura, Hikaru Hashitani","doi":"10.1007/s00441-025-04024-7","DOIUrl":"10.1007/s00441-025-04024-7","url":null,"abstract":"<p><p>Age-related loss of volume and strength of the external urethral sphincter (EUS) is involved in the pathogenesis of stress urinary incontinence. Here, we explored if aged EUS undergoes capillary rarefaction, a characteristic of sarcopenia. Ca²⁺ dynamics in pericytes of EUS capillaries were also investigated to understand their roles in regulating EUS blood supply. Florescence immunohistochemistry was employed to compare the density of striated muscle fibres, fibroblasts, capillaries and pericytes between young and aged female mouse EUS. Electrical filed stimulation (EFS)-induced Ca²⁺ dynamics in EUS capillary pericytes were investigated using young NG2-GCaMP6 mice. In comparison to young EUS, aged EUS displayed a reduced density of striated muscle fibres, an increased density of PDGFRα⁺ fibroblasts, as well as a reduced density of capillaries and pericytes. EFS evoked prolonged Ca²⁺ transients and transient Ca²⁺ reductions in α-smooth muscle actin negative pericytes. The EFS-induced Ca²⁺ transients were prevented by either guanethidine (10 µM) or phentolamine (1 µM), despite the lack of pericapillary tyrosine hydroxylase-positive sympathetic fibres. The EFS-induced Ca²⁺ reductions were prevented by L-nitroarginine (10 µM). Pericytes were not innervated by neuronal NOS (nNOS)-positive nerve fibres, while EUS muscle fibres express sarcolemmal nNOS immunoreactivity. Thus, capillary rarefaction may contribute to the age-related EUS loss associated fibrosis. The Ca²⁺ reductions in pericytes appear to be mediated by NO released from EUS muscle fibres. Since pericytes express ANO1 Ca²⁺-activated chloride channels, the Ca²⁺ reductions would cause hyperpolarisation that spreads to the upstream resulting in arteriolar dilatation to increase blood supply to contracting EUS fibres.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"345-360"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145630553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peroxisomes in the mouse ovary and their alterations during follicular development and oocyte maturation.","authors":"Claudia Colasante, Eva-Maria Distler, Shan Wang, Phillip Grant, Eveline Baumgart-Vogt","doi":"10.1007/s00441-025-04025-6","DOIUrl":"10.1007/s00441-025-04025-6","url":null,"abstract":"<p><p>Peroxisomes are the site of important metabolic pathways as they contain enzymes involved in the β-oxidation of fatty acids, the degradation of reactive oxygen species and the synthesis of ether-lipids and steroid hormone precursors. They are present in virtually all eukaryotic cells, yet they demonstrate considerable heterogeneity of number and protein composition. The presence of peroxisomes and concomitant activity of peroxisomal enzymes was previously documented in ovarian cells and it was suggested that peroxisomal oxidative stress management through catalase plays a pivotal role during oogenesis. In the present article, an exhaustive analysis is presented of the changes in peroxisome abundance during folliculogenesis in mouse oocytes. To this end, a quantitative assessment of the number of peroxisomes was conducted through morphometric analysis in the oocytes in follicular and oestrus cycle stages. Furthermore, a qualitative investigation was undertaken into the distribution of peroxisomal proteins involved in their biogenesis, membrane transport and lipid/hormone and reactive oxygen species metabolism in secondary and tertiary follicles. Given the metabolic connection between peroxisomes and mitochondria, the study was complemented with immunofluorescence stainings for reactive oxygen species and mitochondrial electron transport chain complexes and antioxidative enzymes. Our results confirmed the presence of peroxisomes in all follicular cells, including the oocyte. They further prove that peroxisomal abundance and protein composition are subject to variation depending on follicular and oestrus cycle stage and follicular cell type. Most strikingly, the study provided evidence of the near absence of catalase and of oxidative stress marker 8-oxo-2'-deoxyguanosine in the oocyte.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"373-394"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145586245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extra-oral expression of bitter taste receptors in pigs and their correlation with hepatic cytochrome P450 enzymes.","authors":"Ewelina Pych, Navid Sahebekhtiari, Bo Ekstrand, Martin Krøyer Rasmussen","doi":"10.1007/s00441-025-04021-w","DOIUrl":"10.1007/s00441-025-04021-w","url":null,"abstract":"<p><p>Bitter taste receptors (TAS2Rs), known for their role in oral taste perception, are also expressed in extra-oral tissues, suggesting broader physiological functions. This study examined TAS2R expression in porcine tissues and their potential link to detoxification via cytochrome P450 (CYP) enzymes. Using transcriptome profiling complemented by PCR validation, we examined 14 TAS2Rs and detected 8 receptors across multiple tissues, including tongue, liver, jejunum, duodenum, kidney, colon, ileum, and white adipose. Distinct tissue-specific expression patterns were observed, with the highest diversity and abundance in metabolically active organs. Correlation analysis revealed that TAS2R expression levels were not only strongly associated across tissues but also closely aligned with hepatic CYP content, suggesting a functional relationship. These findings provide novel insights into the extra-oral roles of TAS2Rs in pigs, highlighting their potential involvement in xenobiotic sensing, detoxification, and metabolic regulation.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"255-265"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145444338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of ankyrin 3 (ANK3) promotes malignant behaviors associated with altered adhesion dynamics and actin cytoskeleton remodeling in renal cell carcinoma.","authors":"Keerakarn Somsuan, Artitaya Rongjumnong, Wararat Chiangjong, Atthapan Morchang, Arunothai Wanta, Ratirath Samol, Sirirat Yongrum, Natthiya Sakulsak, Anupong Makeudom, Suttichai Krisanaprakornkit, Sutatip Pongcharoen, Somchai Chutipongtanate, Jatuporn Ngoenkam, Siripat Aluksanasuwan","doi":"10.1007/s00441-025-04019-4","DOIUrl":"10.1007/s00441-025-04019-4","url":null,"abstract":"<p><p>Clear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer, with existing therapies largely ineffective for advanced stages. Ankyrin 3 (ANK3), a protein involved in various cellular processes, has been associated with poor prognosis and immune infiltration in ccRCC. However, its role in ccRCC development and progression remains poorly understood. In the present study, ANK3-knockdown 786-O cells were generated using a shRNA approach. Cellular assays revealed that ANK3 knockdown significantly enhanced cell proliferation, migration, and invasion compared to shControl cells. ANK3-knockdown cells also displayed increased nuclear size and accumulation of G2/M phase of the cell cycle. Proteomic analysis identified 59 significantly altered proteins in ANK3-knockdown cells, primarily involved in cell adhesion and structural integrity. Among these, 10 proteins were significantly associated with overall survival in ccRCC patients. Morphological analysis showed that ANK3-knockdown cells exhibited a more spindle-like shape and increased cellular protrusions (filopodia). Western blot analysis further demonstrated altered expression levels of junctional and cytoskeleton proteins, including decreased E-cadherin and increased ZO-1, ICAM-1, vimentin, and β-actin. Immunofluorescence analysis revealed the accumulation of F-actin in the cytoplasm and filopodia in ANK3-knockdown cells. Additionally, ANK3 knockdown enhanced cell adhesion and aggregation capabilities. Finally, a decrease in ANK3 expression was confirmed in ccRCC tissues (grades I and II) compared to adjacent normal tissues. These findings suggest that ANK3 acts as a potential modulator of cell adhesion and cytoskeleton remodeling in ccRCC and may represent a promising therapeutic target for this malignancy.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"441-459"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145437185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D reconstruction of the ovariole in Nilaparvata lugens (Hemiptera: Delphacidae) by FIB-SEM.","authors":"Ke-Qi Ye, Guan Wang, Jian-Sheng Guo, Chuan-Xi Zhang, Xiao-Ping Yu, Dan-Ting Li","doi":"10.1007/s00441-025-04017-6","DOIUrl":"10.1007/s00441-025-04017-6","url":null,"abstract":"<p><p>The brown planthopper (Nilaparvata lugens) has a high reproductive rate, posing a significant challenge to biological control of rice pests. This is largely due to the physiological and functional traits of its telotrophic ovarioles, which feature a centralized nutrient supply to ensure each oocyte obtains essential basic nutrients. Current studies on insect ovarioles primarily focus on hormonal regulation and gene expression; the cellular composition and spatial relationships within N. lugens ovariole tissues remain poorly understood. To illustrate the internal architecture of ovarioles and the intricate oogenesis process at nanoscale resolution, this study employed focused ion beam-scanning electron microscopy for 3D volume reconstruction, overcoming limitations of traditional 2D electron microscopy. Using this advanced imaging technique, we systematically characterized key ovariole components including terminal filaments, trophocytes, germ cell clusters, three types of follicular cells, nutritive cords, and oocytes. Our findings reveal that rather than maintaining a continuous linkage, the nutritive cord transiently connects to the oocytes during specific developmental stages to facilitate nutrient transfer. An assembly of unique, cake-like phospholipoglycoproteins was identified within the oocytes, suggesting specialized energy storage mechanisms. The detailed 3D model elucidates the spatial relationship and relative position between various parts of the ovariole, offering novel insights into the mechanisms of oocyte growth and nutrient acquisition. These findings advance foundational knowledge of insect reproductive biology and provide a valuable framework for future research on pest control strategies targeting N. lugens reproduction.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"477-486"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LOXHD1b knockout alters swimming behavior in zebrafish.","authors":"Yoichi Asaoka, Shunsuke Tarumoto, Yoshinobu Hirose, Aya Iwamoto, Masayuki Tokunaga, Takafumi Matsuura, Yosuke Takemoto, Hiroshi Yamashita, Makoto Furutani-Seiki, Kazuma Sugahara","doi":"10.1007/s00441-025-04005-w","DOIUrl":"10.1007/s00441-025-04005-w","url":null,"abstract":"<p><p>Since human inner ear hair cells do not regenerate, the current treatments of hereditary deafness depend on hearing aids or cochlear implant. However, uncovering the functions of genes responsible for hereditary hearing loss is not only useful for their diagnosis but also for developing therapies. The pathogenetic mechanism of human non-syndromic deafness DFNB77 without morphological defects in the inner year caused by LOXHD1 mutations is not fully understood. We introduced zebrafish because the lateral line hair cells are structurally and physiologically similar to the human inner ear hair cells and mutations involved in non-symptomatic hearing loss can be assessed by their swimming behavior. The knock-out (KO) of LOXHD1b gene which is expressed in the lateral line hair cells was generated using the CRISPR-Cas9 system in zebrafish, and its morphological and functional changes were evaluated. As with human patients the LOXHD1b KO zebrafish larvae did not exhibit detectable morphological defects, but showed prolonged water flow sensing time. These results suggest that LOXHD1b plays pivotal roles for the hair cell neural activity and its KO zebrafish mutant serves as a useful model for revealing the molecular mechanisms linking LOXHD with hair cell function and for a drug screening to rescue the swimming phenotype.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"243-254"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antioxidant, N-acetyl-L-cysteine, affects beta cell oxidative stress, insulin secretion, and intracellular signaling pathways in MIN6 cells.","authors":"Meg Schuurman, Rachel B Wilson, Nica Borradaile, Rennian Wang","doi":"10.1007/s00441-025-04020-x","DOIUrl":"10.1007/s00441-025-04020-x","url":null,"abstract":"<p><p>There is intense public interest in the potential of antioxidant supplements as a preventative treatment for conditions associated with increased oxidative stress, such as type 2 diabetes mellitus. However, there is limited evidence regarding the effects of antioxidants on beta cells during physiological and pathological conditions. We examined the direct effects of N-acetyl-L-cysteine (NAC) supplementation on the MIN6 beta cell line under physiological and fatty acid stress conditions. MIN6 cells were cultured in growth medium with or without NAC (physiological conditions) or growth medium plus palmitate with or without NAC (fatty acid stress conditions). We observed that MIN6 cells receiving NAC, under physiological or fatty acid stress conditions, displayed significantly reduced cellular ATP content and oxidative stress without changes to markers of cell proliferation or apoptosis. Regardless of the treatment conditions, NAC lowered basal insulin release with no change to cellular insulin content, yet improved high glucose-stimulated insulin secretion (GSIS) was only observed under physiological conditions. Importantly, phosphorylated AKT was significantly reduced with NAC supplementation under physiological and fatty acid stress conditions, which was inversely proportional to ERK1/2^{Thr202/Tyr204} phosphorylation during fatty acid stress. Our findings provided evidence that NAC directly impacts ATP production and insulin signaling pathways in MIN6 cells. This study highlights the importance of investigating ROS balance in pancreatic beta cells under physiological and pathological conditions to determine if antioxidant therapies can improve functionality without interfering with essential signaling processes.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"267-281"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145408194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ER morphological analysis associated with interstitial cells of Cajal and smooth muscle cells in the murine stomach.","authors":"Hiromi Tamada, Satoshi Iino","doi":"10.1007/s00441-025-04016-7","DOIUrl":"10.1007/s00441-025-04016-7","url":null,"abstract":"<p><p>In the gastrointestinal tract, several modulators are involved in generating complex motility patterns, including smooth muscle cells (SMCs), interstitial cells of Cajal (ICCs), and PDGFRα+ cells. Notably, ICCs are excitable cells that generate characteristic oscillations in pacemaker activity mediated by the cytoplasmic Ca²⁺ concentration. Since the primary source of cytoplasmic Ca²⁺ is the endoplasmic reticulum (ER) or sarcoplasmic reticulum (SR), its distribution and localisation in the cytoplasm may indicate characteristic aspects that help in understanding its physiological functions. Furthermore, caveolae, which are invaginations of the plasma membrane (PM) observed in both ICCs and SMCs, are key sites for Ca²⁺ sparks, and their functions have been well studied in muscle cells. However, the mechanism by which caveolae and ER interact to regulate Ca²⁺ in ICCs remains unclear compared with that in SMCs. In this study, microdomains comprising the PM, caveolae, and ER/SR were analysed using focused ion beam/scanning electron microscopy (FIB/SEM) to provide new insights into ICC functional analysis. Additionally, as mitochondria could regulate local Ca²⁺ concentration, mitochondria-associated membrane (MAM) contacts with the ER were also analysed in ICCs. Novel ER ultrastructures with distinct characteristics and distributions were identified in each cell type by FIB/SEM. Furthermore, reconstructed three-dimensional (3D) images enabled measurement of the distance between the PM and ER, as well as the MAM areas, thus contributing to a better understanding of ICC physiological features. These new morphological insights may help resolve controversial interpretations in physiological and pharmacological studies of ICCs.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"333-344"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145387346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obligate intracellular bacteria and host cell death pathways-the matter of life and death.","authors":"Manuela Szperlinski, Elias Schermuly, Anja Lührmann, Vera Kozjak-Pavlovic","doi":"10.1007/s00441-025-04014-9","DOIUrl":"10.1007/s00441-025-04014-9","url":null,"abstract":"<p><p>To limit the damage caused by pathogenic bacteria, host organisms possess different defense systems and mechanisms for preventing infection, combating the pathogens, and creating a memory that will avert recurrent infections. Pathogens, on the other hand, have developed countermeasures to enable their replication and spreading. For intracellular pathogenic bacteria, the battleground is localized at the cellular level. Different cell types, including phagocytic, epithelial, and endothelial cells, fibroblasts, and trophoblasts, not only are equipped with diverse defense tools, but also provide different microenvironments, such as varying oxygen tension, pH levels, tonicity, and nutrient supply. The outcome of the infection depends on these conditions in conjunction with microbe-derived virulence factors and bacterial microenvironment needs. Here, we will review the current knowledge on how eukaryotic cells fight obligate intracellular bacteria and how these pathogens counteract the host cell defenses, focusing on cell death pathways. Whereas common cellular strategies for dealing with intracellular bacteria exist, there are also unique approaches adjusted to the individual properties of the pathogen.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"413-439"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145470792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phagocytic hemocytes, independent of pericardial cells, modulate cellular immune responses on the dorsal vessel of mosquitoes, including the infection-induced reduction of the heart rate.","authors":"Cole J Meier, Shabbir Ahmed, Tania Y Estévez-Lao, Julián F Hillyer","doi":"10.1007/s00441-025-04011-y","DOIUrl":"10.1007/s00441-025-04011-y","url":null,"abstract":"<p><p>Infection induces the aggregation of hemocytes on the dorsal vessel of mosquitoes. These hemocytes, called periostial hemocytes, phagocytose pathogens and produce immune factors on the abdominal portion of the dorsal vessel, called the heart. One of these immune factors, nitric oxide, is a pleiotropic free radical that is an antimicrobial and a heartbeat reducer. But nitric oxide is not just produced by hemocytes. It is also synthesized by pericardial cells that flank the heart, and other tissues. To determine whether it is the periostial hemocytes or the pericardial cells that modulate the heart following infection, we chemically ablated the hemocytes using clodronate liposomes and measured immune responses and heart physiology. We demonstrate that clodronate liposomes ablate the sessile hemocytes, including the periostial hemocytes, while leaving the pericardial cells and heart integrity unaffected. Moreover, ablating hemocytes abolishes the phagocytosis of bacteria, alters the deposition of melanized bacteria, and decreases nitric oxide synthase activity on the heart. Importantly, hemocyte ablation eliminates the infection induced reduction of the heart rate, mainly by modifying the anterograde heart rate. Therefore, periostial hemocytes drive immune responses on the heart and infection-induced changes to circulatory physiology.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"173-188"},"PeriodicalIF":2.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12594744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}