Cell and Tissue Research最新文献_第9页

Human mesenchymal stem cell secretomes: Factors affecting profiling and challenges in clinical application 人类间充质干细胞分泌物：影响分析的因素和临床应用的挑战

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-01-20 DOI: 10.1007/s00441-023-03857-4

Noor Anastasha Balqis Noor Azlan, Vieralynda Vitus, Nurshamimi Nor Rashid, Fazlina Nordin, Gee Jun Tye, Wan Safwani Wan Kamarul Zaman

{"title":"Human mesenchymal stem cell secretomes: Factors affecting profiling and challenges in clinical application","authors":"Noor Anastasha Balqis Noor Azlan, Vieralynda Vitus, Nurshamimi Nor Rashid, Fazlina Nordin, Gee Jun Tye, Wan Safwani Wan Kamarul Zaman","doi":"10.1007/s00441-023-03857-4","DOIUrl":"https://doi.org/10.1007/s00441-023-03857-4","url":null,"abstract":"The promising field of regenerative medicine is thrilling as it can repair and restore organs for various debilitating diseases. Mesenchymal stem cells are one of the main components in regenerative medicine that work through the release of secretomes. By adopting the use of the secretome in cell-free-based therapy, we may be able to address the challenges faced in cell-based therapy. As one of the components of cell-free-based therapy, secretome has the advantage of a better safety and efficacy profile than mesenchymal stem cells. However, secretome has its challenges that need to be addressed, such as its bioprocessing methods that may impact the secretome content and its mechanisms of action in clinical settings. Effective and standardization of bioprocessing protocols are important to ensure the supply and sustainability of secretomes for clinical applications. This may eventually impact its commercialization and marketability. In this review, the bioprocessing methods and their impacts on the secretome profile and treatment are discussed. This improves understanding of its fundamental aspects leading to potential clinical applications. ","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"21 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139507812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PDLIM1 interacts with HK2 to promote gastric cancer progression through enhancing the Warburg effect via Wnt/β-catenin signaling. PDLIM1与HK2相互作用，通过Wnt/β-catenin信号增强Warburg效应，促进癌症进展。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-01-01 Epub Date: 2023-11-06 DOI: 10.1007/s00441-023-03840-z

Yunpeng Lei, Lirui He, Yue Li, Jianing Hou, Haoran Zhang, Guan Li

{"title":"PDLIM1 interacts with HK2 to promote gastric cancer progression through enhancing the Warburg effect via Wnt/β-catenin signaling.","authors":"Yunpeng Lei, Lirui He, Yue Li, Jianing Hou, Haoran Zhang, Guan Li","doi":"10.1007/s00441-023-03840-z","DOIUrl":"10.1007/s00441-023-03840-z","url":null,"abstract":"PDZ and LIM domain protein 1 (PDLIM1) is a cytoskeletal protein and is associated with the malignant pathological features of several tumors. However, the prognostic value of PDLIM1 and the molecular mechanisms by which it is involved in the metabolism and progression in gastric cancer (GC) are still unclear. The GEPIA database was used to predict the expression and prognosis of PDLIM1 in GC. qRT-PCR and western blot assays were applied to detect the mRNA and protein expression in GC tissues and cells. Loss- and gain-of-function experiments were performed to evaluate the biological role of PDLIM1 in GC cells. The Warburg effect was detected by a battery of glycolytic indicators. The interaction of PDLIM1 and hexokinase 2 (HK2) was determined by a co-immunoprecipitation assay. Furthermore, the modulatory effects of PDLIM1 and HK2 on Wnt/β-catenin signaling were assessed. The results showed that PDLIM1 expression was upregulated in GC tissues and cells and was associated with a poor prognosis for GC patients. PDLIM1 inhibition reduced GC cell proliferation, migration and invasion and promoted cell apoptosis. In the glucose deprivation (GLU-D) condition, the PDLIM1 level was reduced and PDLIM1 overexpression led to an increase in glycolysis. Besides, mechanistic investigation showed that PDLIM1 interacted with HK2 to mediate biological behaviors and the glycolysis of GC through Wnt/β-catenin signaling under glucose deprivation. In conclusion, PDLIM1 interacts with HK2 to promote gastric cancer progression by enhancing the Warburg effect via Wnt/β-catenin signaling.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"105-116"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71478516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Huogu injection protects against SONFH by promoting osteogenic differentiation of BMSCs and preventing osteoblast apoptosis. 活骨注射液通过促进骨髓间充质干细胞成骨分化和阻止成骨细胞凋亡来预防SONFH。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-01-01 Epub Date: 2023-12-02 DOI: 10.1007/s00441-023-03846-7

Xin Zhang, Ziyu Li, Xilin Xu, Zhao Liu, Yuanyuan Hao, Fubiao Yang, Xiaodong Li, Ning Zhang, Yunlong Hou, Xiaofeng Zhang

{"title":"Huogu injection protects against SONFH by promoting osteogenic differentiation of BMSCs and preventing osteoblast apoptosis.","authors":"Xin Zhang, Ziyu Li, Xilin Xu, Zhao Liu, Yuanyuan Hao, Fubiao Yang, Xiaodong Li, Ning Zhang, Yunlong Hou, Xiaofeng Zhang","doi":"10.1007/s00441-023-03846-7","DOIUrl":"10.1007/s00441-023-03846-7","url":null,"abstract":"To investigate the effect and mechanism of Huogu injection (HG) on steroid-induced osteonecrosis of the femoral head (SONFH), we established a SONFH model in rabbits using horse serum and dexamethasone (DEX) and applied HG locally at the hip joint. We evaluated the therapeutic efficacy at 4 weeks using scanning electron microscopy (SEM), micro-CT, and qualitative histology including H&E, Masson's trichrome, ALP, and TUNEL staining. In vitro, we induced osteogenic differentiation of bone marrow stromal cells (BMSCs) and performed analysis on days 14 and 21 of cell differentiation. The findings, in vivo, including SEM, micro-CT, and H&E staining, showed that HG significantly maintained bone quality and trabecular number. ALP staining indicated that HG promoted the proliferation of bone cells. Moreover, the results of Masson's trichrome staining demonstrated the essential role of HG in collagen synthesis. Additionally, TUNEL staining revealed that HG reduced apoptosis. ALP and ARS staining in vitro confirmed that HG enhanced osteogenic differentiation and mineralization, consistent with the WB and qRT-PCR analysis. Furthermore, Annexin V-FITC/PI staining verified that HG inhibited osteoblast apoptosis, in agreement with the WB and qRT-PCR analyses. Furthermore, combined with the UPLC analysis, we found that naringin enhanced the osteogenic differentiation and accelerated the deposition of calcium phosphate. Salvianolic acid B protected osteoblasts derived from BMSCs against GCs-mediated apoptosis. Thus, this study not only reveals the mechanism of HG in promoting osteogenesis and anti-apoptosis of osteoblasts but also identifies the active-related components in HG, by which we provide the evidence for the application of HG in SONFH.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"63-79"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10774174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138469977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and ultrastructure of digestive system in pre-zoea and zoea I larvae of red king crab, Paralithodes camtschaticus (Tilesius, 1815). 红王蟹，Paralithodes camtschaticus (Tilesius, 1815)，幼体和幼虫消化系统的形态和超微结构。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-01-01 Epub Date: 2023-12-02 DOI: 10.1007/s00441-023-03843-w

Nadezhda V Kalacheva, Talia T Ginanova, Yaroslav O Kamenev, Sergey I Maslennikov, Igor Yu Dolmatov

{"title":"Morphology and ultrastructure of digestive system in pre-zoea and zoea I larvae of red king crab, Paralithodes camtschaticus (Tilesius, 1815).","authors":"Nadezhda V Kalacheva, Talia T Ginanova, Yaroslav O Kamenev, Sergey I Maslennikov, Igor Yu Dolmatov","doi":"10.1007/s00441-023-03843-w","DOIUrl":"10.1007/s00441-023-03843-w","url":null,"abstract":"The digestive system structure in pre-zoea and zoea I larvae of the red king crab Paralithodes camtschaticus has been examined. During this development period, the digestive system consists of an esophagus, a stomach, a midgut (where the hepatopancreas ducts open), and a hindgut. The esophagus begins from the oral slit on the animal's ventral side and extends vertically up to the junction with the cardiac stomach. The latter is followed by the pyloric stomach. At the stages under study, crabs have a cardiac-pyloric valve and a pyloric filter in the stomach already developed. The midgut begins with an expansion in the cephalothorax, enters the pleon, grows narrower there, and extends to somite 3 of pleon. The hepatopancreas is represented by a symmetrical paired gland which occupies almost the entire cephalothorax space and opens with its ducts at the junction of the pyloric stomach with the midgut. The hepatopancreas is divided into the anterior and posterior lobes. At the pre-zoea stage, the anterior lobes are large and filled with yolk. At the zoea I stage, the anterior lobes are smaller relative to the entire hepatopancreas, and the posterior lobes increase and form tubular outgrowths. It has been shown that during the transition from pre-zoea to zoea I, the number of mitochondria in enterocytes increases and a peritrophic membrane forms in the midgut. These changes are probably associated with the transition to independent living and feeding.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"1-20"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138469978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution, quantification, and characterization of substance P enteric neurons in the submucosal and myenteric plexuses of the porcine colon. 猪结肠粘膜下和肌丛P物质肠神经元的分布、定量和表征。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-01-01 Epub Date: 2023-11-20 DOI: 10.1007/s00441-023-03842-x

Maurizio Mazzoni, Luis Cabanillas, Anna Costanzini, Filippo Caremoli, Mulugeta Million, Muriel Larauche, Paolo Clavenzani, Roberto De Giorgio, Catia Sternini

{"title":"Distribution, quantification, and characterization of substance P enteric neurons in the submucosal and myenteric plexuses of the porcine colon.","authors":"Maurizio Mazzoni, Luis Cabanillas, Anna Costanzini, Filippo Caremoli, Mulugeta Million, Muriel Larauche, Paolo Clavenzani, Roberto De Giorgio, Catia Sternini","doi":"10.1007/s00441-023-03842-x","DOIUrl":"10.1007/s00441-023-03842-x","url":null,"abstract":"The pig is an important translational model for studying intestinal physiology and disorders for its many homologies with humans, including the organization of the enteric nervous system (ENS), the major regulator of gastrointestinal functions. This study focused on the quantification and neurochemical characterization of substance P (SP) neurons in the pig ascending (AC) and descending colon (DC) in wholemount preparations of the inner submucosal plexus (ISP), outer submucosal plexus (OSP), and myenteric plexus (MP). We used antibodies for the pan-neuronal marker HuCD, and choline acetyltransferase (ChAT) and neuronal nitric oxide synthase (nNOS), markers for excitatory and inhibitory transmitters, for multiple labeling immunofluorescence and high-resolution confocal microscopy. The highest density of SP immunoreactive (IR) neurons was in the ISP (222/mm2 in the AC, 166/mm2 in the DC), where they make up about a third of HuCD-IR neurons, compared to the OSP and MP (19-22% and 13-17%, respectively, P < 0.001-0.0001). HuCD/SP/ChAT-IR neurons (up to 23%) were overall more abundant than HuCD/SP/nNOS-IR neurons (< 10%). Most SP-IR neurons contained ChAT-IR (62-85%), whereas 18-38% contained nNOS-IR with the highest peak in the OSP. A subpopulation of SP-IR neurons contains both ChAT- and nNOS-IR with the highest peak in the OSP and ISP of DC (33-36%) and the lowest in the ISP of AC (< 10%, P < 0.001). SP-IR varicose fibers were abundant in the ganglia. This study shows that SP-IR neurons are functionally distinct with variable proportions in different plexuses in the AC and DC reflecting diverse functions of specific colonic regions.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"39-51"},"PeriodicalIF":3.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10774220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138046215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical characterisation of the adult Nothobranchius furzeri intestine. 成虫furzeri nothobranchus肠道的免疫组织化学特征。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-01-01 Epub Date: 2023-11-28 DOI: 10.1007/s00441-023-03845-8

Janina Borgonovo, Camilo Allende-Castro, Danilo B Medinas, Deyanira Cárdenas, María Paz Cuevas, Claudio Hetz, Miguel L Concha

{"title":"Immunohistochemical characterisation of the adult Nothobranchius furzeri intestine.","authors":"Janina Borgonovo, Camilo Allende-Castro, Danilo B Medinas, Deyanira Cárdenas, María Paz Cuevas, Claudio Hetz, Miguel L Concha","doi":"10.1007/s00441-023-03845-8","DOIUrl":"10.1007/s00441-023-03845-8","url":null,"abstract":"Nothobranchius furzeri is emerging as an exciting vertebrate organism in the field of biomedicine, developmental biology and ecotoxicology research. Its short generation time, compressed lifespan and accelerated ageing make it a versatile model for longitudinal studies with high traceability. Although in recent years the use of this model has increased enormously, there is still little information on the anatomy, morphology and histology of its main organs. In this paper, we present a description of the digestive system of N. furzeri, with emphasis on the intestine. We note that the general architecture of the intestinal tissue is shared with other vertebrates, and includes a folding mucosa, an outer muscle layer and a myenteric plexus. By immunohistochemical analysis, we reveal that the mucosa harbours the same type of epithelial cells observed in mammals, including enterocytes, goblet cells and enteroendocrine cells, and that the myenteric neurons express neurotransmitters common to other species, such as serotonin, substance P and tyrosine hydroxylase. In addition, we detect the presence of a proliferative compartment at the base of the intestinal folds. The description of the normal intestinal morphology provided here constitutes a baseline information to contrast with tissue alterations in future lines of research assessing pathologies, ageing-related diseases or damage caused by toxic agents.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"21-38"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneity of endothelial VE-PTP downstream polarization, Tie2 activation, junctional claudin-5, and permeability in the aorta and vena cava. 主动脉和腔静脉内皮VE-PTP下游极化、Tie2激活、连接claudin-5和通透性的异质性。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-01-01 Epub Date: 2023-11-30 DOI: 10.1007/s00441-023-03844-9

Peter Baluk, Keisuke Shirakura, Dietmar Vestweber, Donald M McDonald

{"title":"Heterogeneity of endothelial VE-PTP downstream polarization, Tie2 activation, junctional claudin-5, and permeability in the aorta and vena cava.","authors":"Peter Baluk, Keisuke Shirakura, Dietmar Vestweber, Donald M McDonald","doi":"10.1007/s00441-023-03844-9","DOIUrl":"10.1007/s00441-023-03844-9","url":null,"abstract":"Endothelial cells of mammalian blood vessels have multiple levels of heterogeneity along the vascular tree and among different organs. Further heterogeneity results from blood flow turbulence and variations in shear stress. In the aorta, vascular endothelial protein tyrosine phosphatase (VE-PTP), which dephosphorylates tyrosine kinase receptor Tie2 in the plasma membrane, undergoes downstream polarization and endocytosis in endothelial cells exposed to laminar flow and high shear stress. VE-PTP sequestration promotes Tie2 phosphorylation at tyrosine992 and endothelial barrier tightening. The present study characterized the heterogeneity of VE-PTP polarization, Tie2-pY992 and total Tie2, and claudin-5 in anatomically defined regions of endothelial cells in the mouse descending thoracic aorta, where laminar flow is variable and IgG extravasation is patchy. We discovered that VE-PTP and Tie2-pY992 had mosaic patterns, unlike the uniform distribution of total Tie2. Claudin-5 at tight junctions also had a mosaic pattern, whereas VE-cadherin at adherens junctions bordered all endothelial cells. Importantly, the amounts of Tie2-pY992 and claudin-5 in aortic endothelial cells correlated with downstream polarization of VE-PTP. VE-PTP and Tie2-pY992 also had mosaic patterns in the vena cava, but claudin-5 was nearly absent and extravasated IgG was ubiquitous. Correlation of Tie2-pY992 and claudin-5 with VE-PTP polarization supports their collective interaction in the regulation of endothelial barrier function in the aorta, yet differences between the aorta and vena cava indicate additional flow-related determinants of permeability. Together, the results highlight new levels of endothelial cell functional mosaicism in the aorta and vena cava, where blood flow dynamics are well known to be heterogeneous.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"81-103"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10774230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental roles of glomerular epithelial protein-1 in mice molar morphogenesis. 肾小球上皮蛋白-1在小鼠磨牙形态发生中的发育作用。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-01-01 Epub Date: 2023-11-21 DOI: 10.1007/s00441-023-03841-y

Sanjiv Neupane, Yam Prasad Aryal, Hee-Jin Kwak, Sung-Gwon Lee, Tae-Young Kim, Elina Pokharel, Ji-Youn Kim, Jung-Hyeuk Kim, Wern-Joo Sohn, Seo-Young An, Chang-Hyeon An, Jae-Kwang Jung, Jung-Hong Ha, Hitoshi Yamamoto, Sung-Won Cho, Sanggyu Lee, Youngkyun Lee, Kwang-Kyun Park, Bong-Ki Min, Chungoo Park, Tae-Yub Kwon, Sung-Jin Cho, Jae-Young Kim

{"title":"Developmental roles of glomerular epithelial protein-1 in mice molar morphogenesis.","authors":"Sanjiv Neupane, Yam Prasad Aryal, Hee-Jin Kwak, Sung-Gwon Lee, Tae-Young Kim, Elina Pokharel, Ji-Youn Kim, Jung-Hyeuk Kim, Wern-Joo Sohn, Seo-Young An, Chang-Hyeon An, Jae-Kwang Jung, Jung-Hong Ha, Hitoshi Yamamoto, Sung-Won Cho, Sanggyu Lee, Youngkyun Lee, Kwang-Kyun Park, Bong-Ki Min, Chungoo Park, Tae-Yub Kwon, Sung-Jin Cho, Jae-Young Kim","doi":"10.1007/s00441-023-03841-y","DOIUrl":"10.1007/s00441-023-03841-y","url":null,"abstract":"Glomerular epithelial protein-1 (Glepp1), a R3 subtype family of receptor-type protein tyrosine phosphatases, plays important role in the activation of Src family kinases and regulates cellular processes such as cell proliferation, differentiation, and apoptosis. In this study, we firstly examined the functional evaluation of Glepp1 in tooth development and morphogenesis. The precise expression level and developmental function of Glepp1 were examined by RT-qPCR, in situ hybridization, and loss and gain of functional study using a range of in vitro organ cultivation methods. Expression of Glepp1 was detected in the developing tooth germs in cap and bell stage of tooth development. Knocking down Glepp1 at E13 for 2 days showed the altered expression levels of tooth development-related signaling molecules, including Bmps, Dspp, Fgf4, Lef1, and Shh. Moreover, transient knock down of Glepp1 revealed alterations in cellular physiology, examined by the localization patterns of Ki67 and E-cadherin. Similarly, knocking down of Glepp1 showed disrupted enamel rod and interrod formation in 3-week renal transplanted teeth. In addition, due to attrition of odontoblastic layers, the expression signals of Dspp and the localization of NESTIN were almost not detected after knock down of Glepp1; however, their expressions were increased after Glepp1 overexpression. Thus, our results suggested that Glepp1 plays modulating roles during odontogenesis by regulating the expression levels of signaling molecules and cellular events to achieve the proper structural formation of hard tissue matrices in mice molar development.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"53-62"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new technology for the treatment of premature ovarian insufficiency: Cell sheet. 一种治疗卵巢早衰的新技术:细胞片。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-01-01 Epub Date: 2023-12-05 DOI: 10.1007/s00441-023-03848-5

Pinar Calis, Gokcenur Arik, Cemile Merve Seymen, Gokce Kaynak Bayrak, Ozge Ekin Akdere, Canan Yilmaz, Atiye Seda Yar Saglam, Menemşe Gümüşderelioğlu, Gulnur Take Kaplanoglu

{"title":"A new technology for the treatment of premature ovarian insufficiency: Cell sheet.","authors":"Pinar Calis, Gokcenur Arik, Cemile Merve Seymen, Gokce Kaynak Bayrak, Ozge Ekin Akdere, Canan Yilmaz, Atiye Seda Yar Saglam, Menemşe Gümüşderelioğlu, Gulnur Take Kaplanoglu","doi":"10.1007/s00441-023-03848-5","DOIUrl":"10.1007/s00441-023-03848-5","url":null,"abstract":"Premature ovarian insufficiency (POI) is defined as the development of hypergonadotropic hypogonadism before the age of 40 with definitive treatment being absent. In the current study, we aim to compare the efficacy of the cell sheet method with an intravenous (IV) application of adipose-derived mesenchymal stem cells (AdMSCs) to the POI with an animal model. In the current prospective study, 6-to-8-week-old Sprague Dawley rats were generated four groups: (i) a control group in which only PBS was administered; (ii) an only-POI group generated by cyclophosphamide; (iii) a POI group treated by way of IV AdMSCs; and (iv) a POI group treated by way of the cell sheet method. Twenty-eight days after an oophorectomy was performed, intracardiac blood was taken. Follicle count, immunohistochemical examination for GDF9, BMP15, and TUNEL were conducted, gene expressions of GDF9 and BMP15 were examined, and E2 was measured in the serum samples. With hematoxylin-eosin, in the third group, multi oocytes follicles were the most remarkable finding. In the fourth group, most of the follicles presented normal morphology. GDF9 involvement was similar between the first and fourth groups. BMP-15 immunoreactivity, in contrast to fourth group, was weak in all stages in the second and third groups. The current attempt represents a pioneer study in the literature in which a cell sheet method is used for the first time in a POI model. These results suggest that the cell sheet method may be a feasible and efficient method for the stem cell treatment of models with POI and could be a new treatment approach in POI.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"117-131"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138482064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wharton’s jelly mesenchymal stem cells transplantation for critical limb ischemia in patients with type 2 diabetes mellitus: a preliminary report of phase I clinical trial 沃顿果冻间充质干细胞移植治疗 2 型糖尿病患者严重肢体缺血：I 期临床试验初步报告

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2023-12-19 DOI: 10.1007/s00441-023-03854-7

Mohammad Taghi Ashoobi, Hossein Hemmati, Hamid Reza Aghayan, Zeinab Zarei-Behjani, Samaneh Keshavarz, Hamideh Babaloo, Saman Maroufizadeh, Saeed Yousefi, Mohaya Farzin, Elham Vojoudi

{"title":"Wharton’s jelly mesenchymal stem cells transplantation for critical limb ischemia in patients with type 2 diabetes mellitus: a preliminary report of phase I clinical trial","authors":"Mohammad Taghi Ashoobi, Hossein Hemmati, Hamid Reza Aghayan, Zeinab Zarei-Behjani, Samaneh Keshavarz, Hamideh Babaloo, Saman Maroufizadeh, Saeed Yousefi, Mohaya Farzin, Elham Vojoudi","doi":"10.1007/s00441-023-03854-7","DOIUrl":"https://doi.org/10.1007/s00441-023-03854-7","url":null,"abstract":"Peripheral artery disease (PAD) affects more than 230 million people worldwide, with approximately 11% of patients presenting with advanced-stage PAD or critical limb ischemia (CLI). To avoid or delay amputation, particularly in no-option CLI patients with infeasible or ineffective revascularization, new treatment strategies such as regenerative therapies should be developed. Mesenchymal stem cells (MSCs) are the most popular cell source in regenerative therapies. They possess significant characteristics such as angiogenic, anti-inflammatory, and immunomodulatory activities, which encourage their application in different diseases. This phase I clinical trial reports the safety, feasibility, and probable efficacy of the intramuscular administration of allogeneic Wharton’s jelly-derived MSCs (WJ-MSCs) in type 2 diabetes patients with CLI. Out of six screened patients with CLI, five patients were administered WJ-MSCs into the gastrocnemius, soleus, and the proximal part of the tibialis anterior muscles of the ischemic lower limb. The safety of WJ-MSCs injection was considered a primary outcome. Secondary endpoints included wound healing, the presence of pulse at the disease site, the absence of amputation, and improvement in visual analogue scale (VAS), pain-free walking time, and foot and ankle disability index (FADI). No patient experienced adverse events and foot or even toe amputation during the 6-month follow-up. Six months after the intervention, there were a significantly lower VAS score and significantly higher pain-free walking time and FADI score than the baseline, but no statistically significant difference was seen between other time points. In conclusion, allogeneic WJ-MSC transplantation in patients with CLI seems to be safe and effective.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"4 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138741705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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