Cell and Tissue Research最新文献_第8页

CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics. 大鼠垂体中间叶的CD9/ sox2阳性细胞表现出间充质干细胞的特征。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-14 DOI: 10.1007/s00441-024-03947-x

Ayano Shindo, Morio Azuma, Ken Fujiwara, Saishu Yoshida, Kotaro Horiguchi

{"title":"CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics.","authors":"Ayano Shindo, Morio Azuma, Ken Fujiwara, Saishu Yoshida, Kotaro Horiguchi","doi":"10.1007/s00441-024-03947-x","DOIUrl":"10.1007/s00441-024-03947-x","url":null,"abstract":"Adult tissue stem cells of the anterior pituitary gland, CD9/SOX2-positive cells, are believed to exist in the marginal cell layer (MCL) bordering the residual lumen of the Rathke's pouch. These cells migrate from the intermediate lobe side of the MCL (IL-MCL) to the anterior lobe side of the MCL and may be involved in supplying hormone-producing cells. Previous studies reported that some SOX2-positive cells of the anterior lobe differentiate into skeletal muscle cells. These findings suggest that CD9/SOX2-positive cells in the anterior pituitary have mesenchymal stem cell (MSC) properties. To substantiate this hypothesis, we examined whether CD9-positive cells isolated from IL-MCL of adult male rats differentiate into mesenchymal cells, such as endothelial cells, adipocytes, chondrocytes, and osteocytes. Immunohistochemical analysis revealed that the CD9-positive cells were positive for the MSC markers, CD349, CD105, CD271, and CD273 and were detected in the early postnatal period at the boundary between the posterior and intermediate lobes but not in the embryonic period. In addition, some adult tissue stem cells derived from neural crest cells and bone marrow haematopoietic stem cells were positive for both CD9 and MSC markers, indicating that several CD9/SOX2-positive cells in the IL-MCL of the pituitary gland are MSCs that invaded from external tissues during pituitary development in the early postnatal period and exist in the adult tissue stem cells as suppliers of hormone-producing and endothelial cells in the anterior lobe. These findings should have implications for the application of CD9/SOX2-positive cells in regenerative therapy of the pituitary.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"277-290"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic memory as crucial contributing factor in directing the differentiation of human iPSC into pancreatic β-cells in vitro. 表观遗传记忆是体外诱导人iPSC向胰腺β细胞分化的重要因素。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-30 DOI: 10.1007/s00441-025-03952-8

Abdoulaye Diane, Razik Bin Abdul Mu-U-Min, Heba Hussain Al-Siddiqi

{"title":"Epigenetic memory as crucial contributing factor in directing the differentiation of human iPSC into pancreatic β-cells in vitro.","authors":"Abdoulaye Diane, Razik Bin Abdul Mu-U-Min, Heba Hussain Al-Siddiqi","doi":"10.1007/s00441-025-03952-8","DOIUrl":"10.1007/s00441-025-03952-8","url":null,"abstract":"Impaired insulin secretion contributes to the pathogenesis of type 1 diabetes mellitus through autoimmune destruction of pancreatic β-cells and the pathogenesis of severe forms of type 2 diabetes mellitus through β-cell dedifferentiation and other mechanisms. Replenishment of malfunctioning β-cells via islet transplantation has the potential to induce long-term glycemic control in the body. However, this treatment option cannot widely be implemented in clinical due to healthy islet donor shortage. Emerging β-cell replacement with human-induced pluripotent stem cell (iPSC) provides high remedial therapy hopes. Thus, tremendous progress has been made in developing β-cell differentiation protocols in vitro; however, most of the differentiated iPSC-derived β-cells showed immature phenotypes associated with low efficiency depending on the iPSC lines used, creating a crucial barrier for their clinical implementation. Multiple mechanisms including differences in genetic, cell cycle patterns, and mitochondrial dysfunction underlie the defective differentiation propensity of iPSC into insulin-producing β-cells. Accumulating evidence recently indicated that, following the reprogramming, epigenetic memory inherited from parental cells substantially affects the differentiation capacity of many iPSC lines. Therefore, differences in epigenetic signature are likely to be essential contributing factors influencing the propensity of iPSC differentiation. In this review, we will document the impact of the epigenome on the reprogramming efficacy and differentiation potential of iPSCs and how targeting the epigenetic residual memory could be an additional strategy to improve the differentiation efficiency of existing protocols to generate fully functional hPSC-derived pancreatic β-cells for diabetes therapy and drug screening.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"267-276"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis. 淡水小龙虾造血组织的组织与表达分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-04 DOI: 10.1007/s00441-024-03943-1

Thanapong Kruangkum, Kenneth Söderhäll, Irene Söderhäll

{"title":"The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis.","authors":"Thanapong Kruangkum, Kenneth Söderhäll, Irene Söderhäll","doi":"10.1007/s00441-024-03943-1","DOIUrl":"10.1007/s00441-024-03943-1","url":null,"abstract":"The hematopoietic tissue (HPT) and anterior proliferation center (APC) are the main hemocyte-producing organs of the freshwater crayfish, Pacifastacus leniusculus. To deepen our understanding of immune responses to various pathogens, it is essential to identify distinct hemocyte subpopulations with specific functions and to further explore how these cells are generated. Here we provide an in-depth histological study of the HPT and APC in order to localize cell types in different developmental stages, and to provide some information regarding the hemocyte differentiation in the crayfish. We localized mRNA expression of previously identified genes in the HPT/APC and hemocytes by RNA-FISH. The expression of hemolectin and transglutaminase 1 was shown to be co-localized in a high number of the HPT cells, while transglutaminase 2 was expressed in different cell types mainly associated with epithelium or endothelium. Furthermore, by double RNA-FISH for hemolectin and a previously unidentified PDGF-like factor, combined with immunostaining for prophenoloxidase, we could identify several different subtypes of hemocytes, indicating that the immune function of hemocytes in crayfish is more diversified and complex than previously appreciated.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"303-322"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and histochemical characteristics of the foregut, midgut, and hindgut, and their alterations during ovarian development in female freshwater prawn, Macrobrachium rosenbergii. 罗氏沼虾雌虾前、中、后肠形态和组织化学特征及其在卵巢发育过程中的变化。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-13 DOI: 10.1007/s00441-024-03948-w

Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul

{"title":"Morphological and histochemical characteristics of the foregut, midgut, and hindgut, and their alterations during ovarian development in female freshwater prawn, Macrobrachium rosenbergii.","authors":"Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul","doi":"10.1007/s00441-024-03948-w","DOIUrl":"10.1007/s00441-024-03948-w","url":null,"abstract":"The anatomical, histological, and histochemical characteristics of the foregut (FG), midgut (MG), and hindgut (HG), as well as their alterations during the ovarian cycle in female prawns, Macrobrachium rosenbergii, were investigated. The esophagus (ESO), cardia (CD), and pylorus (PY) are the main components of the FG. An epithelium (Ep) with thick cuticle (Cu) layers lining the ESO, and the ESO is encircled by the ESO glands. The CD has a thick musculature, whereas the Ep of the PY are characterized by numerous villi and columnar Ep cells with a thinner layer of Cu. The inner longitudinal (LM) and the outer circular (CM) muscles were both present in the PY. The MG is lined by Ep cells which are connected to the basement membrane, and it lacks Cu. Microvilli, and subapical vacuoles are visible on the apical surface of Ep cells of the MG. The outermost layer is characterized by a dense strip of elastic fibers and a cluster of collagen fibers. The HG has the Ep cells with a thin Cu layer, and the HG glands form a rosette-like structure. The HG is surrounded by the CM and the LM fibers. The reactivities of Periodic Acid Schiff and Alcian Blue in these digestive organs altered throughout the ovarian cycle, and this was supported by the increased expression of mucin levels as ovarian maturation progressed. Our results offer novel and significant insights into the anatomical and histochemical structures of these digestive organs, and demonstrate a significant correlation between ovarian development and feeding in the female prawn, M. rosenbergii.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"351-375"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform. 使用Xenium平台对人类骨骼肌进行原位空间转录组学分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-09 DOI: 10.1007/s00441-024-03945-z

Nejc Umek, Marija Meznarič, Žiga Šink, Kaja Blagotinšek Cokan, Uršula Prosenc Zmrzljak, Simon Horvat

{"title":"In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform.","authors":"Nejc Umek, Marija Meznarič, Žiga Šink, Kaja Blagotinšek Cokan, Uršula Prosenc Zmrzljak, Simon Horvat","doi":"10.1007/s00441-024-03945-z","DOIUrl":"10.1007/s00441-024-03945-z","url":null,"abstract":"Traditional transcriptomic studies often overlook the complex heterogeneity of skeletal muscle, as they typically isolate RNA from mixed muscle fibre and cell populations, resulting in an averaged transcriptomic profile that obscures fibre type-specific differences. This study assessed the potential of the recently developed Xenium platform for high-resolution spatial transcriptomic analysis of human skeletal muscle histological sections. Human vastus lateralis muscle samples from two individuals were analysed using the Xenium platform and Human Multi-Tissue and Cancer Panel targeting 377 genes complemented by staining of successive sections for Myosin Heavy Chain isoforms to differentiate between type 1 and type 2 muscle fibres. Manual segmentation of muscle fibres allowed accurate comparisons of transcript densities across fibre types and subcellular regions, overcoming limitations in the platform's automated segmentation. The analysis revealed higher transcript density in type 1 fibres, particularly in nuclear and perinuclear areas, and identified 191 out of 377 genes with differential expression between muscle fibres and perimysium. Genes such as PROX1, S100A1, LGR5, ACTA2, and LPL exhibited higher expression in type 1 fibres, whereas PEBP4, CAVIN1, GATM, and PVALB in type 2 fibres. We demonstrated that the Xenium platform is capable of high-resolution spatial in situ transcriptomic analysis of skeletal muscle histological sections. This study demonstrates that, with manual segmentation, the Xenium platform effectively performs fibre type-specific transcriptomic analysis, providing new insights into skeletal muscle biology.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"291-302"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles support increased expansion of mesenchymal stromal cells on fetal membrane-derived decellularized extracellular matrix. 细胞外囊泡支持胚胎膜来源的脱细胞细胞外基质上间充质间质细胞的增加。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2024-12-24 DOI: 10.1007/s00441-024-03946-y

Aida Shakouri-Motlagh, Andrea J O'Connor, Shaun P Brennecke, Daniel E Heath, Bill Kalionis

{"title":"Extracellular vesicles support increased expansion of mesenchymal stromal cells on fetal membrane-derived decellularized extracellular matrix.","authors":"Aida Shakouri-Motlagh, Andrea J O'Connor, Shaun P Brennecke, Daniel E Heath, Bill Kalionis","doi":"10.1007/s00441-024-03946-y","DOIUrl":"10.1007/s00441-024-03946-y","url":null,"abstract":"Decidual mesenchymal stromal cells (DMSC) were the source of extracellular vesicles (DMSC_EV). The xCELLigence real-time cell growth assay revealed increasing concentrations of EVs decreased DMSC attachment in the early growth phase but stimulated DMSC proliferation at day 7 when grown on tissue culture plastic (TCP). DMSC attachment and proliferation varied depending on the growth surface and DMSC_EV supplementation. DMSC attachment increased on decellularized and solubilized amniotic (s-dAM) whether or not EVs were added. Only Matrigel substrate increased DMSC attachment with added EVs. The addition of EVs increased DMSC proliferation only on the s-dAM substrate. DMSCs were more motile on s-dAM and decellularized and solubilized chorionic (s-dCM) membranes following EV addition. The osteogenic potential of DMSCs was improved on s-dAM substrates when supplanted with EVs. Finally, the levels of reactive oxygen species in DMSCs varied depending on the substrate but not on added EVs. We show that the addition of in vitro EVs isolated from the source being expanded (i.e., DMSCs) and the presence of ECM improve DMSC behaviours during ex vivo expansion. The inclusion of two key components of the MSC niche, EVs and ECM, benefitted the ex vivo expansion of MSCs. Added in vitro EVs increased the proliferation of DMSCs when grown on s-dAM but not on s-dCM, whereas they improved DMSC mobility on both surfaces. Testing different ECMs could be used to promote specific desired characteristics of DMSCs, and different combinations of EVs and ECM may enhance desirable MSC characteristics for specific therapeutic settings.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"323-336"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A brief history of insect neuropeptide and peptide hormone research. 昆虫神经肽和肽激素研究简史。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-01 Epub Date: 2024-12-10 DOI: 10.1007/s00441-024-03936-0

Dick R Nässel

{"title":"A brief history of insect neuropeptide and peptide hormone research.","authors":"Dick R Nässel","doi":"10.1007/s00441-024-03936-0","DOIUrl":"10.1007/s00441-024-03936-0","url":null,"abstract":"This review briefly summarizes 50 years of research on insect neuropeptide and peptide hormone (collectively abbreviated NPH) signaling, starting with the sequencing of proctolin in 1975. The first 25 years, before the sequencing of the Drosophila genome, were characterized by efforts to identify novel NPHs by biochemical means, mapping of their distribution in neurons, neurosecretory cells, and endocrine cells of the intestine. Functional studies of NPHs were predominantly dealing with hormonal aspects of peptides and many employed ex vivo assays. With the annotation of the Drosophila genome, and more specifically of the NPHs and their receptors in Drosophila and other insects, a new era followed. This started with matching of NPH ligands to orphan receptors, and studies to localize NPHs with improved detection methods. Important advances were made with introduction of a rich repertoire of innovative molecular genetic approaches to localize and interfere with expression or function of NPHs and their receptors. These methods enabled cell- or circuit-specific interference with NPH signaling for in vivo assays to determine roles in behavior and physiology, imaging of neuronal activity, and analysis of connectivity in peptidergic circuits. Recent years have seen a dramatic increase in reports on the multiple functions of NPHs in development, physiology and behavior. Importantly, we can now appreciate the pleiotropic functions of NPHs, as well as the functional peptidergic \"networks\" where state dependent NPH signaling ensures behavioral plasticity and systemic homeostasis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"129-159"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles. 基于窦卵泡黄体化细胞的卵巢黄体三维体外模型的建立。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-01 Epub Date: 2024-12-19 DOI: 10.1007/s00441-024-03937-z

Michał M Hryciuk, Filip Schröter, Svenja Claaßen, Christine Aurich, Jella Wauters, Celina Haße, Beate C Braun

{"title":"Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles.","authors":"Michał M Hryciuk, Filip Schröter, Svenja Claaßen, Christine Aurich, Jella Wauters, Celina Haße, Beate C Braun","doi":"10.1007/s00441-024-03937-z","DOIUrl":"10.1007/s00441-024-03937-z","url":null,"abstract":"The study aimed to establish a long-term 3D cell culture model using luteinized follicular cells to investigate the functionality and life cycle of the CL in felids. A mixture of cell types from antral follicles was luteinized in vitro and cultured for up to 23 days. The method, initially applied to the domestic cat, was later extended to Persian and Clouded leopards. Antral follicles were isolated and digested with enzymes; then, the cells were subjected to culture. Experimental subsets were treated with/without 1 µg/mL cloprostenol to validate the cell culture model's suitability for functional studies. In domestic cat samples, microscopic evaluation indicated luteinization, which was confirmed by increased progestagen concentrations and IHC staining for HSD3B and CYP11A1. The gene expression of selected steroidogenic factors (HSD3B1, STAR, CYP11A1) and hormone receptors (LHCGR, PTGFR, PRLR) significantly increased, while CYP17A1 expression decreased. Cloprostenol treatment resulted in reduction of steroidogenic activity, proving its suitability for functional studies. Persian and Clouded leopards' cell cultures exhibited similar patterns in progestagen secretion and gene expression, compared to domestic cats. This model, with its defined luteinization, as well as high and stable progestagen production, allows future investigation of factors regulating CL life cycle and function.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"211-229"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foxa1 disruption enhances human cell integration in human-mouse interspecies chimeras. Foxa1的破坏增强了人-小鼠种间嵌合体中人类细胞的整合。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-01 Epub Date: 2024-12-21 DOI: 10.1007/s00441-024-03941-3

Li-Na Wang, Jun-Shuang Jia, Xing-Long Yang, Yue-Ting Wen, Jing-Xian Liu, Deng-Ke Li, Xing-Rui Chen, Jia-Hong Wang, Ji-Ke Li, Zhong-Xi Huang, Kai-Tai Yao

{"title":"Foxa1 disruption enhances human cell integration in human-mouse interspecies chimeras.","authors":"Li-Na Wang, Jun-Shuang Jia, Xing-Long Yang, Yue-Ting Wen, Jing-Xian Liu, Deng-Ke Li, Xing-Rui Chen, Jia-Hong Wang, Ji-Ke Li, Zhong-Xi Huang, Kai-Tai Yao","doi":"10.1007/s00441-024-03941-3","DOIUrl":"10.1007/s00441-024-03941-3","url":null,"abstract":"Blastocyst complementation can potentially generate a rodent model with humanized nasopharyngeal epithelium (NE) that supports sustained Epstein-Barr virus (EBV) infection, enabling comprehensive studies of EBV biology in nasopharyngeal carcinoma. However, during this process, the specific gene knockouts required to establish a developmental niche for NE remain unclear. We performed bioinformatics analyses and generated Foxa1 mutant mice to confirm that Foxa1 disruption could potentially create a developmental niche for NE. Subsequently, MYD88-inactivated human pluripotent stem cells (hPSCs) were constructed and complemented with Foxa1-deficient mouse blastocysts, with Nosip-deficient mouse blastocysts as a control. The chimerism of human cells in mouse embryos was evaluated from E8.5 to E12.5 using genomic DNA PCR and immunohistochemistry. Our bioinformatics analysis indicated that the expression patterns of Foxa1 in E8.5 to E16.5 mouse embryos underscore its critical role in NE development. The generated mice with Foxa1 disordered region mutations displayed morphological abnormality in NE, suggesting Foxa1-knockouts could potentially establish a developmental niche for NE. In chimeric assays, human cells integrated into 80.00% of Foxa1-deficient embryos, compared with the 4.17% in controls. Immunohistochemistry results revealed robust proliferation of human cells in Foxa1-deficient mouse embryos. However, chimeras from Foxa1-deficient mouse embryos did not survive beyond E10.5, hindering the evaluation of human cell integration in mouse NE. Foxa1 disruption in mouse embryos significantly enhances the integration of human cells in human-mouse interspecies chimeras, thereby facilitating the generation of endoderm-derived organs through blastocyst complementation. Overcoming chimeras' embryonic lethality is crucial for successfully generating humanized NE in Foxa1-deficient mouse embryos.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"231-245"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancer Enh483 regulates myoblast proliferation and differentiation of buffalo myoblasts by targeting FAXC. 增强子Enh483通过靶向FAXC调节水牛肌母细胞的增殖和分化。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-02-01 Epub Date: 2024-12-17 DOI: 10.1007/s00441-024-03944-0

Yaling Chen, Jiahui Zhao, Cuiwei Zhong, Yujin Kang, Zhaocheng Xiong, Jieping Huang, Zhipeng Li, Qingyou Liu, Deshun Shi, Xinxin Li, Jian Wang, Hui Li

{"title":"Enhancer Enh483 regulates myoblast proliferation and differentiation of buffalo myoblasts by targeting FAXC.","authors":"Yaling Chen, Jiahui Zhao, Cuiwei Zhong, Yujin Kang, Zhaocheng Xiong, Jieping Huang, Zhipeng Li, Qingyou Liu, Deshun Shi, Xinxin Li, Jian Wang, Hui Li","doi":"10.1007/s00441-024-03944-0","DOIUrl":"10.1007/s00441-024-03944-0","url":null,"abstract":"A detailed understanding of the precise regulatory mechanisms governing buffalo skeletal muscle is crucial for improving meat quality and yield. Proper skeletal muscle fate decisions necessitate the accurate regulation of key enhancers. This study screened nine potential enhancers linked to muscle development by analysing ATAC-seq data from buffalo myoblasts during the proliferative and differentiative phases. The enhancer activity of these candidates was confirmed in buffalo myoblasts, C2C12, and human skeletal muscle myoblasts using a dual-luciferase reporter system. The CRISPRi system and RT-qPCR were used to test the effects of 9 candidate enhancers on buffalo myoblasts. The active enhancer, Enh483, was selected based on its significant impact. Upon successful inhibition of Enh483 using CRISPRi, decreases in the expression of buffalo myogenic proliferation marker genes (PCNA, CyclinD1, and CDK2) were observed via RT-qPCR and Western blot. Subsequent proliferation assays using CCK-8 and EdU confirmed the promotive effect of Enh483 on buffalo myogenic cell proliferation. Following a 5-day differentiation induction period, changes in the expression of differentiation marker genes (MyoD1, MyoG, and MyHC) were analysed using RT-qPCR and Western blot. Additionally, fused myotube numbers were quantified, and the impact of Enh483 on buffalo myogenic cell differentiation was assessed through immunofluorescence. Our findings indicate that Enh483 facilitates buffalo myogenic cell differentiation. Further interaction analysis utilising 3C-PCR revealed a direct association between Enh483 and the FAXC promoter. In summary, the results from this study lay a foundational framework for deciphering the intricate regulatory mechanisms underpinning buffalo muscle development.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"161-171"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0