Cell and Tissue Research最新文献_第8页

Glycogen shunt is essential for submandibular gland morphogenesis. 糖原分流是颌下腺形态发生所必需的。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-10 DOI: 10.1007/s00441-025-04033-6

Hiroko Ida-Yonemochi, Yuki Ohno, Hayato Ohshima

{"title":"Glycogen shunt is essential for submandibular gland morphogenesis.","authors":"Hiroko Ida-Yonemochi, Yuki Ohno, Hayato Ohshima","doi":"10.1007/s00441-025-04033-6","DOIUrl":"10.1007/s00441-025-04033-6","url":null,"abstract":"Glycogen metabolism is an important pathway in energy metabolism, and when cells require energy, glycogen is broken down into glucose-1-phosphate through glycogenolysis, which serves as an intracellular energy source. Recently, the concept of \"glycogen shunt\" has been proposed to regulate the synthesis, accumulation, and breakdown of glycogen to provide glucose at the appropriate time, particularly in organogenesis. This study demonstrated the timing of the localization of glycogen and molecules related to glycogen metabolism during submandibular gland development using embryonic and postnatal mice. In addition, a glycogenolysis inhibition experiment was conducted in organ culture systems of submandibular gland tissues. From embryonic day 13.5 (E13.5), glycogen synthesis started in the salivary epithelial cells, and glycogen accumulation and degradation occurred at E15.5. Conversely, in the submandibular gland tissue around birth, the number of glycogen-retained cells increased, and active glycogen synthesis and degradation occurred in acinar cells and terminal tubules. In an in vitro organ culture experiment, early branching morphogenesis was disturbed by a glycogen phosphorylase inhibitor, which significantly inhibited differentiation into acinar and myoepithelial cells. These results suggest the important role of the glycogen shunt in early growth and cell differentiation during submandibular gland development.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"1"},"PeriodicalIF":3.1,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kidney organoids in translational research: disease modeling, drug discovery, and unresolved challenges. 转化研究中的肾类器官：疾病建模、药物发现和未解决的挑战。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 Epub Date: 2025-11-05 DOI: 10.1007/s00441-025-04018-5

Yue Xi, Wei Song

{"title":"Kidney organoids in translational research: disease modeling, drug discovery, and unresolved challenges.","authors":"Yue Xi, Wei Song","doi":"10.1007/s00441-025-04018-5","DOIUrl":"10.1007/s00441-025-04018-5","url":null,"abstract":"Kidney organoids derived from human pluripotent stem cells (hPSCs) have emerged as powerful platforms for translational nephrology, enabling complex renal pathophysiology modeling in physiologically relevant three-dimensional contexts. This review synthesizes recent advances in kidney organoid applications for disease modeling and drug discovery, highlighting their translational potential beyond developmental biology. These organoids recapitulate key human kidney architectural features, including nephron-like structures with glomeruli and tubules, while exhibiting greater cellular heterogeneity than traditional two-dimensional cultures. They effectively model monogenic renal disorders including autosomal dominant polycystic kidney disease (ADPKD), congenital nephrotic syndrome, and Alport syndrome, as well as acquired conditions like acute kidney injury and drug-induced nephrotoxicity. Kidney organoids serve as predictive nephrotoxicity screening platforms, demonstrating dose- and time-dependent responses to cisplatin, tenofovir, and aristolochic acid. However, significant challenges persist, including insufficient vascularization, developmental immaturity, segmental bias, absent urinary drainage systems, and reproducibility variability. Emerging bioengineering strategies-including endothelial co-culture, microfluidic integration, and 3D bioprinting-aim to address these limitations. Integrating stem cell biology with engineering innovations and multi-omics technologies will be crucial for refining kidney organoids into scalable, reproducible models that faithfully recapitulate human kidney physiology and disease, ultimately enabling their translation into precision medicine applications.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"303-312"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145444284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen II and decellularized hyaline cartilage scaffolds derived from bovine trachea differentially promote chondrogenic differentiation of mesenchymal stem cells and decrease secretion of angiogenic factors. 来源于牛气管的II型胶原和去细胞透明软骨支架在促进间充质干细胞成软骨分化和减少血管生成因子分泌方面存在差异。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 Epub Date: 2025-12-17 DOI: 10.1007/s00441-025-04027-4

Adriana M Flórez, Ronald A Jiménez, María A Torres, Mara E M Braga, Herminio C de Sousa, Marta R Fontanilla

{"title":"Collagen II and decellularized hyaline cartilage scaffolds derived from bovine trachea differentially promote chondrogenic differentiation of mesenchymal stem cells and decrease secretion of angiogenic factors.","authors":"Adriana M Flórez, Ronald A Jiménez, María A Torres, Mara E M Braga, Herminio C de Sousa, Marta R Fontanilla","doi":"10.1007/s00441-025-04027-4","DOIUrl":"10.1007/s00441-025-04027-4","url":null,"abstract":"Collagen II scaffolds and decellularized cartilage are used for tissue engineering; however, there are no studies that compare their properties. To this aim, this study produced and characterized collagen II and decellularized cartilage scaffolds made from bovine trachea and evaluated the influence of the culture medium on the tissue type synthesized by human bone marrow mesenchymal stem cells (hBMSC) and human adipose mesenchymal stem cells (hASC) cultured on the scaffolds. Three angiogenic factors secreted by these cell cultures were also quantified. Decellularized hyaline cartilage had lower concentrations of collagen II and higher concentrations of GAG than collagen II scaffolds. The porosity, pore size, and fluid sorption capacity of the collagen scaffolds were greater than those of decellularized hyaline cartilage. Both scaffolds were hydrophilic, and their surfaces were negatively charged. The enzymatic degradation and Young's and compression moduli of decellularized cartilage were higher than those of collagen II scaffolds. hBMSC and hASC cultured on collagen II and decellularized cartilage scaffolds with chondrogenic differentiation medium synthesized different percentages of the tissue types that made up the extracellular matrix. hBMSC on decellularized hyaline cartilage produced mainly hyaline cartilage-like tissue, whereas hASC had more immature transitional tissue. When cells were seeded onto collagen II scaffolds, transitional and fibrous tissue prevailed over hyaline tissue. Our data demonstrated that stem cell chondrogenesis in vitro was more favored by decellularized hyaline cartilage than by collagen II scaffolds, and that the concentration of angiopoietin-1, VEGF and bFGF decreased with increasing hyaline tissue formation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"313-331"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145767243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smarter stem cells: how AI is supercharging iPSC technology. 更智能的干细胞：人工智能如何强化iPSC技术。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 Epub Date: 2025-08-14 DOI: 10.1007/s00441-025-03999-7

Hany E Marei

{"title":"Smarter stem cells: how AI is supercharging iPSC technology.","authors":"Hany E Marei","doi":"10.1007/s00441-025-03999-7","DOIUrl":"10.1007/s00441-025-03999-7","url":null,"abstract":"Integrated with artificial intelligence (AI), induced pluripotent stem cell (iPSC) technology could enhance disease modeling, cellular biology, regenerative medicine, and pharmaceutical development. AI has enhanced iPSC differentiation, cultural conditions, and speed of disease-specific model development. Furthermore, AI-based massive omics database analysis exposes hidden biological tendencies, enhancing customized treatment. Investigating new AI algorithms will enable one to solve problems, including interpretability and data quality, resulting from AI's interaction with iPSC technology. These advances fundamentally alter stem cell research and therapeutic applications, therefore facilitating the emergence of regenerative medicine and precision healthcare. AI has evolved in biomedical research into a transformational technology unique in great data analysis, predictive modeling, and automation capacity. AI integration increases the development of patient-specific cell types for disease modeling, pharmacological research, and regenerative medicine by substantially improving IPSC-based technologies. Emphasizing changes in disease models, alternative methodologies, and cellular reprogramming, this work examines current advancements in the use of AI in iPSC technology. The argument on significant obstacles and possibilities reveals how AI could alter the objectives of iPSC research and implementation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"283-301"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144844749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Antler stem cells as a novel stem cell source for reducing liver fibrosis. 更正：鹿角干细胞作为一种新的干细胞来源，可减少肝纤维化。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 DOI: 10.1007/s00441-025-04023-8

Xiaoli Rong, Yanyan Yang, Guokun Zhang, Haiying Zhang, Chunyi Li, Yimin Wang

引用次数: 0

Fibulin-4 is required for the mechanical stability of tendons. 纤维蛋白-4对肌腱的机械稳定性是必需的。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 Epub Date: 2025-11-13 DOI: 10.1007/s00441-025-04028-3

Takako Sasaki, Kyoko Jin, Aki Takimoto, Yuki Taga, Yoshihiro Ishikawa, Hans Peter Bächinger, Takao Sakai, Takeshi Terabayashi, Katsuhiro Hanada, Ursula Schlötzer-Schrehardt, Chisa Shukunami, Yuji Hiraki, Tomoyuki Nakamura, Ei Yamamoto

{"title":"Fibulin-4 is required for the mechanical stability of tendons.","authors":"Takako Sasaki, Kyoko Jin, Aki Takimoto, Yuki Taga, Yoshihiro Ishikawa, Hans Peter Bächinger, Takao Sakai, Takeshi Terabayashi, Katsuhiro Hanada, Ursula Schlötzer-Schrehardt, Chisa Shukunami, Yuji Hiraki, Tomoyuki Nakamura, Ei Yamamoto","doi":"10.1007/s00441-025-04028-3","DOIUrl":"10.1007/s00441-025-04028-3","url":null,"abstract":"Fibulin-4, an extracellular matrix protein, is indispensable for elastic fiber assembly. Fibulin-4 null mice show bilateral forelimb contracture and patients with EFEMP2/FBLN4 mutations demonstrate similar defects, besides joint laxity, vascular and pulmonary abnormalities. Here we report that limb tendons in fibulin-4 null mice developed normally until E17.5-18.5, but thereafter　thinner tendons showed abnormalities, suggesting that fibulin-4 maintains the integrity of certain tendons. Tendon/ligament specific conditional knockout mice of Efemp2/Fbln4 (ScxCre-H;Fbln4flox/- mice) were generated in order to elucidate its role of collagen fibril organization, collagen cross-linking and mechanical features of tendons. Curiously, however, the conditional Fbln4 knockout mice did not show forelimb contractures or other obvious morphological defects. We could detect small amounts of fibulin-4 in tendon extracts, but isolated tenocytes from the conditional knockout mice did not secrete fibulin-4, confirming that the Efemp2/Fbln4 gene was properly deleted in tenocytes. Electron microscopic analyses revealed an enhanced proportion of thinner fibrils in tendons from the conditional knockout mice. Furthermore, mechanical stress tests of patellar tendons of the conditional knockout mice revealed strongly reduced strain resistance compared to the tendons of control mice, although cross-link formation and thermal stability of tendon collagen were not affected. These finding indicate that fibulin-4 has an important role in the organization and stability of collagen fibrils in tendons.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"361-372"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145502338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: ER morphological analysis associated with interstitial cells of Cajal and smooth muscle cells in the murine stomach. 更正：与小鼠胃Cajal间质细胞和平滑肌细胞相关的ER形态学分析。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 DOI: 10.1007/s00441-025-04026-5

Hiromi Tamada, Satoshi Iino

引用次数: 0

Disruption of Myd88 in a salmonid epithelioid cell line reveals its contribution to bacterial detection and immune response. 在鲑鱼上皮样细胞系中破坏Myd88揭示了其对细菌检测和免疫反应的贡献。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 Epub Date: 2025-10-23 DOI: 10.1007/s00441-025-04015-8

Alexander Rebl, Mathilde Peruzzi, Catherine Collins, Niccolò Vendramin, Pierre Boudinot, Niels Lorenzen, Bertrand Collet

{"title":"Disruption of Myd88 in a salmonid epithelioid cell line reveals its contribution to bacterial detection and immune response.","authors":"Alexander Rebl, Mathilde Peruzzi, Catherine Collins, Niccolò Vendramin, Pierre Boudinot, Niels Lorenzen, Bertrand Collet","doi":"10.1007/s00441-025-04015-8","DOIUrl":"10.1007/s00441-025-04015-8","url":null,"abstract":"Toll-like receptors (TLRs) are a class of pattern-recognition receptors that recognize pathogen- and damage-associated molecular patterns and initiate immune responses. TLRs selectively recruit distinct adapter molecules such as the myeloid differentiation primary response protein 88 (MyD88) that mediates signaling downstream of all TLRs, with the exception of TLR3. To investigate TLR signaling pathways in fish, we engineered a knockout clonal epitheliod fish cell line, named MYD88C2, using CRISPR/Cas9-mediated genome editing to disrupt the myd88 gene. We characterized the phenotype of this cell line alongside a wild-type cell line through gene-expression profiling and reporter-gene analyses in the context of stimulation with heat-killed Vibrio anguillarum, heat-killed Escherichia coli, flagellin, zymosan, and inoculation with a panel of viruses. We demonstrate that the proinflammatory response to zymosan and flagellin, as measured through the induction of proinflammatory genes, was greatly reduced in the MYD88C2 cell line. The responsiveness to zymosan was found to be partially restored by transfecting the MYD88C2 cell line with a myd88-expression plasmid. In contrast, the loss of the myd88 gene had no impact on the cytopathic effect associated with the replication of viral haemorrhagic septicemia virus (VHSV), infectious haematopoietic necrosis virus (IHNV), infectious pancreatic necrosis virus (IPNV), spring viraemia of carp virus (SVCV) or infectious salmon anaemia virus (ISAV). These findings highlight the critical role of MyD88 in mediating specific proinflammatory responses to bacterial and fungal stimuli, while its absence has no detectable impact on viral replication or cytopathogenicity in epithelioid fish cells under the conditions tested.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"395-411"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catecholaminergic system in the brain of the terrestrial slug Limax valentianus. 陆生蛞蝓脑内的儿茶酚胺能系统。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 Epub Date: 2025-09-19 DOI: 10.1007/s00441-025-04010-z

Ryota Matsuo, Aika Matsuki, Yuko Matsuo

{"title":"Catecholaminergic system in the brain of the terrestrial slug Limax valentianus.","authors":"Ryota Matsuo, Aika Matsuki, Yuko Matsuo","doi":"10.1007/s00441-025-04010-z","DOIUrl":"10.1007/s00441-025-04010-z","url":null,"abstract":"Catecholamines, including dopamine, are thought to play important roles in the nervous system of mollusks. In the brains of the terrestrial gastropods, the procerebrum is the higher olfactory center involved in olfactory associative learning. Dopamine is known to affect the activities of interneurons in the procerebrum. However, little is known about from where and how dopaminergic neurons project to the procerebrum. In the present study, we generated a specific antibody against tyrosine hydroxylase (TH) and visualized catecholaminergic neurons, including dopaminergic neurons, in the central and peripheral nervous systems of the terrestrial slug Limax valentianus. We found that (1) the number of the cell bodies of catecholaminergic neurons was larger in the ventral aspect than in the dorsal aspect of the brain; (2) most of the TH-immunoreactive putative sensory afferents in the superior tentacle projected to the brain along the axis as bundles in a regularly spaced manner while bypassing the procerebrum following entry into the brain; (3) TH-immunoreactive nerves in the procerebrum were derived from at least three distinct origins outside and within the procerebrum; and (4) dopamine upregulated the oscillatory frequency of the local field potential oscillation in the procerebrum, which was occluded by pre-incubation with sulpiride, a mammalian D2/D3 receptor antagonist. This is the first study to investigate the catecholaminergic system, with a special focus on the procerebrum, using a specific antibody against TH in terrestrial gastropods. The present study uncovered catecholaminergic regulation of procerebrum activity through innervation from both the outside and inside of the procerebrum.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"461-475"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Panx1a modulates metabolic stress signaling and synaptic composition in the developing zebrafish brain. Panx1a调节发育中的斑马鱼大脑中的代谢应激信号和突触组成。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-12-01 Epub Date: 2025-11-11 DOI: 10.1007/s00441-025-04022-9

Georg S O Zoidl, Nickie Safarian, Christiane Zoidl, Steven Connor, Georg R Zoidl

{"title":"Panx1a modulates metabolic stress signaling and synaptic composition in the developing zebrafish brain.","authors":"Georg S O Zoidl, Nickie Safarian, Christiane Zoidl, Steven Connor, Georg R Zoidl","doi":"10.1007/s00441-025-04022-9","DOIUrl":"10.1007/s00441-025-04022-9","url":null,"abstract":"Pannexin 1a (Panx1a), a neuronal ATP-release channel, is increasingly recognized for its role in neurodevelopment, yet its contribution to synaptic homeostasis under metabolic stress remains poorly defined. We demonstrate that Panx1a coordinates synaptic and metabolic processes supporting neural circuit stability in the developing zebrafish brain. Using a genetic Panx1a knockout model and pharmacological induction of oxidative stress via MPTP, we reveal that Panx1a loss exacerbates metabolic alterations, reduces extracellular ATP availability, and triggers transcriptional activation of AMPK-mTORC1 signaling, autophagy, and apoptosis. These molecular changes coincide with impaired synaptic gene expression and increased neuronal cell death, particularly in the tectum and pallium. Electrophysiological recordings further show that Panx1a may function as a regulator for preserving local field potential coherence and phase-amplitude coupling, with knockout larvae displaying aberrant oscillatory activity and reduced network adaptability. Our findings identify Panx1a as a regulator of the metabolic-synaptic interface during a vulnerable developmental window and suggest that its ablation could contribute to pathophysiological mechanisms underlying neurodevelopmental disorders.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"217-242"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145488050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0