Cell and Tissue Research最新文献_第8页

Regulation of the gap junction interplay during postnatal development in the rat epididymis. 大鼠附睾产后发育过程中间隙连接相互作用的调控

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-12-01 Epub Date: 2024-10-16 DOI: 10.1007/s00441-024-03919-1

Daniel G Cyr, Cécile Adam, Julie Dufresne, Mary Gregory

{"title":"Regulation of the gap junction interplay during postnatal development in the rat epididymis.","authors":"Daniel G Cyr, Cécile Adam, Julie Dufresne, Mary Gregory","doi":"10.1007/s00441-024-03919-1","DOIUrl":"10.1007/s00441-024-03919-1","url":null,"abstract":"During postnatal development of the rat epididymis, a change in the expression of gap junction proteins, or connexins (Cxs), occurs, in which Gjb2 (Cx26) and Gja1 (Cx43) levels in the proximal epididymis are decreased, while Gjb1 (Cx32), Gjb4 (Cx30.3) and Gjb5 (Cx31.1) levels increase. The mechanism(s) responsible for the switch in Cx expression is unknown. The aim of this study is to identify the mechanisms responsible for the decrease in GJB2 protein levels and the increase in other Cxs during postnatal development. Results indicate that decreased Gjb2 expression for 48 h does not alter the expression of other Cxs in RCE-1 principal cells, suggesting a lack of compensatory expression. Sequence analysis of both Gjb2 and Gjb1 promoters identified common multiple response elements to steroid hormones. Using RCE-1 cells, we observed that dexamethasone increased Gjb2 mRNA levels by twofold after 48 h, while estradiol had no effect. Orchidectomy in rats resulted in a significant increase in GJB2 and decreased GJB1 in the caput and corpus epididymidis. Changes in Cxs protein levels were prevented by testosterone in orchidectomized rats. Similar results were observed in the prostate, another androgen-receptive organ. LNCaP cells, which are androgen-responsive, showed that exogenous dihydrotestosterone (DHT) decreased Gjb2 mRNA levels by approximately 50% concomitant with a 1.5-fold increase in Gjb1 levels. Using a GJB1 promoter construct we showed that DHT could induce transactivation of the luciferase transgene, while transactivation of two GJB2 promoters were unaltered. Results indicate that androgens and glucocorticoids regulate the expression of epididymal Cxs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"191-206"},"PeriodicalIF":3.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type 2 vomeronasal receptor expression in the olfactory organ of African lungfish, Protopterus annectens. 非洲肺鱼（Protopterus annectens）嗅觉器官中 2 型绒毛膜受体的表达。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-11-01 Epub Date: 2024-09-30 DOI: 10.1007/s00441-024-03918-2

Shoko Nakamuta, Zicong Zhang, Masato Nikaido, Takuya Yokoyama, Yoshio Yamamoto, Nobuaki Nakamuta

{"title":"Type 2 vomeronasal receptor expression in the olfactory organ of African lungfish, Protopterus annectens.","authors":"Shoko Nakamuta, Zicong Zhang, Masato Nikaido, Takuya Yokoyama, Yoshio Yamamoto, Nobuaki Nakamuta","doi":"10.1007/s00441-024-03918-2","DOIUrl":"10.1007/s00441-024-03918-2","url":null,"abstract":"The olfactory organ of tetrapods, with few exceptions, comprises the main and accessory organs: olfactory epithelium (OE) and vomeronasal organ (VNO). Unlike tetrapods, teleost fish lack a VNO. However, lungfish, a type of sarcopterygian fish closely related to tetrapods, possesses a lamellar OE similar to the OE of teleosts and a recess epithelium (RecE) resembling the amphibian VNO. The RecE has been hypothesized as a primordial VNO. Olfactory receptors in tetrapods are distinctively expressed in the OE and VNO. For instance, type 2 vomeronasal receptors (V2Rs) in Xenopus are categorized into those exclusively expressed in the OE and those solely expressed in the VNO. It remains unclear whether V2Rs are differentially expressed between the lamellar OE and RecE in lungfish. This study investigated V2R expression in the lamellar OE and RecE of the African lungfish, Protopterus annectens. P. annectens V2Rs were categorized into three groups: those exclusively expressed in the lamellar OE, those exclusively expressed in the RecE, and those expressed in both the lamellar OE and RecE. V2Rs exclusively expressed in the RecE and those expressed in both the lamellar OE and RecE formed a distinct clade in the phylogenetic tree, whereas others were solely expressed in the lamellar OE. These findings suggest that lungfish V2R expression represents an intermediate stage toward complete segregation between V2Rs expressed in the OE and those expressed in the VNO.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"79-91"},"PeriodicalIF":3.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142342440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of BCAT1 expression improves recurrent miscarriage by regulating cellular dysfunction and inflammation of trophoblasts. 抑制BCAT1的表达可通过调节滋养细胞的细胞功能障碍和炎症改善复发性流产。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-11-01 Epub Date: 2024-10-02 DOI: 10.1007/s00441-024-03921-7

Guangli Xu, Chao Tian, Yanru Li, Lei Fang, Jing Wang, Zhuqing Jing, Simeng Li, Ping Chen

{"title":"Inhibition of BCAT1 expression improves recurrent miscarriage by regulating cellular dysfunction and inflammation of trophoblasts.","authors":"Guangli Xu, Chao Tian, Yanru Li, Lei Fang, Jing Wang, Zhuqing Jing, Simeng Li, Ping Chen","doi":"10.1007/s00441-024-03921-7","DOIUrl":"10.1007/s00441-024-03921-7","url":null,"abstract":"Sustained or chronic inflammation in the placenta can result in placental insufficiency, leading to adverse reproductive outcomes such as pregnancy loss. Branched-chain amino acid transaminase 1 (BCAT1) expresses in the placenta and is involved in the pathological inflammatory response, but its role in recurrent miscarriage (RM) has not been fully investigated. In the present study, we delved into the effects of BCAT1 on trophoblast inflammation induced by lipopolysaccharide (LPS) and a mouse model of pregnancy loss induced by LPS. In vitro, after the HTR-8/SVneo cells were treated with LPS and BCATc inhibitor 2 (a selective BCAT inhibitor), the cell apoptosis was verified by TUNEL assay, and the activity of caspase-3 and caspase-9 was detected. Real-time PCR, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence (IF) were used to determine the expression of inflammatory cytokines (TNF-α, IL-6, and IL-1β) and inflammasomes (NLRP3 and ASC) in LPS-treated trophoblast cells. Western blot analysis was performed to verify the expression of phospho-IκBα (p-IκBα) in cells and NF-κB p65 in the nuclei. IF staining was used to detect the nuclear translocation of NF-κB p65. The DNA binding activity of NF-κB was detected by an electrophoretic mobility shift assay (EMSA). The results demonstrated that inhibition of BCAT1 reduced trophoblast apoptosis, suppressed the release of proinflammatory cytokines, and prevented NLRP3 inflammasome activation in response to LPS. Additionally, BCAT1 inhibition blocked the activation of the NF-κB pathway in trophoblasts. This study highlights the potential therapeutic role of targeting BCAT1 in preventing adverse reproductive outcomes associated with chronic placental inflammation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"111-121"},"PeriodicalIF":3.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in-depth review of the function of RNA-binding protein FXR1 in neurodevelopment. 深入评述 RNA 结合蛋白 FXR1 在神经发育中的功能。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-11-01 Epub Date: 2024-08-19 DOI: 10.1007/s00441-024-03912-8

Natasha M Méndez-Albelo, Soraya O Sandoval, Zhiyan Xu, Xinyu Zhao

引用次数: 0

Spatial arrangement, polarity, and posttranslational modifications of the microtubule system in the Drosophila eye. 果蝇眼睛中微管系统的空间排列、极性和翻译后修饰。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-11-01 Epub Date: 2024-08-17 DOI: 10.1007/s00441-024-03914-6

Piotr Kos, Otto Baumann

{"title":"Spatial arrangement, polarity, and posttranslational modifications of the microtubule system in the Drosophila eye.","authors":"Piotr Kos, Otto Baumann","doi":"10.1007/s00441-024-03914-6","DOIUrl":"10.1007/s00441-024-03914-6","url":null,"abstract":"We have analyzed the organization of the microtubule system in photoreceptor cells and pigment cells within the adult Drosophila compound eye. Immunofluorescence localization of tubulin and of Short stop, a spectraplakin that has been reported to be involved in the anchorage of microtubule minus ends at the membrane, suggests the presence of non-centrosomal microtubule-organizing centers at the distal tip of the visual cells. Ultrastructural analyses confirm that microtubules emanate from membrane-associated plaques at the site of contact with cone cells and that all microtubules are aligned in distal-proximal direction within the photoreceptor cells. Determination of microtubule polarities demonstrated that about 95% of the microtubules in photoreceptor cells are oriented with their plus end in the direction of the synapse. Pigment cells in the eye contain only microtubules aligned in distal-proximal direction, with their plus end pointing towards the retinal floor. There, two populations of microtubules can be distinguished, single microtubules and bundled microtubules, the latter associated with actin filaments. Whereas microtubules in both photoreceptor cells and pigment cells are acetylated and mono/bi-glutamylated on α-tubulin, bundled microtubules in pigment cells are apparently also mono/bi-glutamylated on β-tubulin, providing the possibility of binding different microtubule-associated proteins.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"123-137"},"PeriodicalIF":3.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study on a unique architecture of the brook lamprey liver and that of the hagfish and banded houndshark liver. 关于溪灯鱼肝脏独特结构与哈吉鱼和带钩鱼肝脏结构的比较研究。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-11-01 Epub Date: 2024-10-01 DOI: 10.1007/s00441-024-03917-3

Noriaki Ota, Haruka Hirose, Yuji Yamazaki, Hideaki Kato, Kazuho Ikeo, Junri Sekiguchi, Sachie Matsubara, Hayato Kawakami, Nobuyoshi Shiojiri

{"title":"Comparative study on a unique architecture of the brook lamprey liver and that of the hagfish and banded houndshark liver.","authors":"Noriaki Ota, Haruka Hirose, Yuji Yamazaki, Hideaki Kato, Kazuho Ikeo, Junri Sekiguchi, Sachie Matsubara, Hayato Kawakami, Nobuyoshi Shiojiri","doi":"10.1007/s00441-024-03917-3","DOIUrl":"10.1007/s00441-024-03917-3","url":null,"abstract":"Although the liver of the lamprey, a group of cyclostomes that diverged the earliest among vertebrates, has abundant bile ducts in the larval stage, which degenerate during metamorphosis, there is no comparative study on its architecture with other early diverged vertebrates in terms of the morphological evolution of vertebrate livers. The present study was undertaken to compare the characteristics of the brook lamprey liver with those of the hagfish and banded houndshark, which have the portal triad type liver architecture, and to discuss its evolution. Although the liver of the brook lamprey had two-cell cords of hepatocytes lined by sinusoids in the ammocoetes larval stage, intrahepatic bile ducts around portal veins penetrated into the liver parenchyma with convolution and gradual reduction in diameter. They also faced dilated sinusoids. The epithelial cells had characteristic intercellular spaces. These characteristics were distinct from those of bile ducts in the hagfish and banded houndshark livers. Although the liver architectures of the hagfish and banded houndshark were similar, the latter penetrated the intrahepatic bile ducts more deeply along the portal veins than the former, in which intrahepatic bile ducts were restricted near the hilum. After metamorphosis, bile ducts degenerated in brook lampreys. These data indicate that the liver architecture of the ammocoetes larva is unique in the parenchymal distribution of bile ducts, their sinusoidal facing, and morphology among extant vertebrates. The periportal distribution of intrahepatic biliary structures may have been established prior to the divergence of the cyclostomes and gnathostomes.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"93-110"},"PeriodicalIF":3.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142342439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complex neurochemistry of the cockroach antennal heart. 蟑螂触角心脏的复杂神经化学。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.1007/s00441-024-03915-5

Hans Agricola, Peter Bräunig

引用次数: 0

Diabetes compromises tight junction protein claudin 14 in the urinary bladder. 糖尿病会损害膀胱中的紧密连接蛋白 Claudin 14。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-10-01 Epub Date: 2024-08-20 DOI: 10.1007/s00441-024-03908-4

Soumitra Mohanty, John Kerr White, Andrea Scheffschick, Berenice Fischer, Anuj Pathak, Jonas Tovi, Claes-Göran Östenson, Pontus Aspenström, Hanna Brauner, Annelie Brauner

{"title":"Diabetes compromises tight junction protein claudin 14 in the urinary bladder.","authors":"Soumitra Mohanty, John Kerr White, Andrea Scheffschick, Berenice Fischer, Anuj Pathak, Jonas Tovi, Claes-Göran Östenson, Pontus Aspenström, Hanna Brauner, Annelie Brauner","doi":"10.1007/s00441-024-03908-4","DOIUrl":"10.1007/s00441-024-03908-4","url":null,"abstract":"Infections are common in patients with diabetes. Moreover, increasing incidence of antibiotic resistance impedes the complete bacterial clearance and calls for alternative treatment strategies. Along with antibacterial resistance, compromised host conditions create a favorable condition for the disease progression. In particular, cell junction proteins are of major importance as they contribute to a tight cell barrier, protecting against invading pathogens. However, the impact of high glucose on cell junction proteins has received little attention in the urinary bladder but merits closer investigation. Here, we report that during diabetes the expression of cell junction protein, claudin 14 is compromised in the human urine exfoliated cells and in the urinary bladder of type 2 diabetic mouse. Further in vitro analysis confirmed a direct correlation of lower intracellular calcium levels with claudin 14 expression in high glucose-treated human uroepithelial cells. Moreover, external calcium supplementation in high glucose-treated cells significantly affected the cell migration and restored the claudin 14 expression through focal adhesion and β-1 integrins. Strengthening the epithelial barrier is essential, especially in individuals with diabetes where basal calcium levels could contribute.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"27-33"},"PeriodicalIF":3.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11424655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of vimentin- and BLBP-positive glial cells and their MMP expression in axonal regeneration after spinal cord transection in goldfish. 波形蛋白和 BLBP 阳性神经胶质细胞及其 MMP 表达参与金鱼脊髓横断后的轴突再生

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI: 10.1007/s00441-024-03907-5

Akihito Takeda, Minami Teshima, Kengo Funakoshi

{"title":"Involvement of vimentin- and BLBP-positive glial cells and their MMP expression in axonal regeneration after spinal cord transection in goldfish.","authors":"Akihito Takeda, Minami Teshima, Kengo Funakoshi","doi":"10.1007/s00441-024-03907-5","DOIUrl":"10.1007/s00441-024-03907-5","url":null,"abstract":"In goldfish, spinal cord injury triggers the formation of a fibrous scar at the injury site. Regenerating axons are able to penetrate the scar tissue, resulting in the recovery of motor function. Previous findings suggested that regenerating axons enter the scar through tubular structures surrounded by glial elements with laminin-positive basement membranes and that glial processes expressing glial fibrillary acidic protein (GFAP) are associated with axonal regeneration. How glia contribute to promoting axonal regeneration, however, is unknown. Here, we revealed that glial processes expressing vimentin or brain lipid-binding protein (BLBP) also enter the fibrous scar after spinal cord injury in goldfish. Vimentin-positive glial processes were more numerous than GFAP- or BLBP-positive glial processes in the scar tissue. Regenerating axons in the scar tissue were more closely associated with vimentin-positive glial processes than GFAP-positive glial processes. Vimentin-positive glial processes co-expressed matrix metalloproteinase (MMP)-14. Our findings suggest that vimentin-positive glial processes closely associate with regenerating axons through tubular structures entering the scar after spinal cord injury in goldfish. In intact spinal cord, ependymo-radial glial cell bodies express BLBP and their radial processes express vimentin, suggesting that vimentin-positive glial processes derive from migrating ependymo-radial glial cells. MMP-14 expressed in vimentin-positive glial cells and their processes might provide a beneficial environment for axonal regeneration.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"15-25"},"PeriodicalIF":3.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy accompanying the developmental process of male germline stem cells. 自噬伴随着男性生殖干细胞的发育过程。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-10-01 Epub Date: 2024-08-14 DOI: 10.1007/s00441-024-03910-w

Zhuofei Jiang, Liji Chen, Tao Wang, Jie Zhao, Shuxian Liu, Yating He, Liyun Wang, Hongfu Wu

{"title":"Autophagy accompanying the developmental process of male germline stem cells.","authors":"Zhuofei Jiang, Liji Chen, Tao Wang, Jie Zhao, Shuxian Liu, Yating He, Liyun Wang, Hongfu Wu","doi":"10.1007/s00441-024-03910-w","DOIUrl":"10.1007/s00441-024-03910-w","url":null,"abstract":"Germline stem cells are a crucial type of stem cell that can stably pass on genetic information to the next generation, providing the necessary foundation for the reproduction and survival of organisms. Male mammalian germline stem cells are unique cell types that include primordial germ cells and spermatogonial stem cells. They can differentiate into germ cells, such as sperm and eggs, thereby facilitating offspring reproduction. In addition, they continuously generate stem cells through self-renewal mechanisms to support the normal function of the reproductive system. Autophagy involves the use of lysosomes to degrade proteins and organelles that are regulated by relevant genes. This process plays an important role in maintaining the homeostasis of germline stem cells and the synthesis, degradation, and recycling of germline stem cell products. Recently, the developmental regulatory mechanism of germline stem cells has been further elucidated, and autophagy has been shown to be involved in the regulation of self-renewal and differentiation of germline stem cells. In this review, we introduce autophagy accompanying the development of germline stem cells, focusing on the autophagy process accompanying the development of male spermatogonial stem cells and the roles of related genes and proteins. We also briefly outline the effects of autophagy dysfunction on germline stem cells and reproduction.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"1-14"},"PeriodicalIF":3.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0