Cell and Tissue Research最新文献_第8页

Identification of vagal afferent nerve endings in the mouse colon and their spatial relationship with enterochromaffin cells. 鉴定小鼠结肠中的迷走传入神经末梢及其与肠粘膜细胞的空间关系。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-06-01 Epub Date: 2024-02-22 DOI: 10.1007/s00441-024-03879-6

Nick J Spencer, Melinda A Kyloh, Lee Travis, Timothy J Hibberd

{"title":"Identification of vagal afferent nerve endings in the mouse colon and their spatial relationship with enterochromaffin cells.","authors":"Nick J Spencer, Melinda A Kyloh, Lee Travis, Timothy J Hibberd","doi":"10.1007/s00441-024-03879-6","DOIUrl":"10.1007/s00441-024-03879-6","url":null,"abstract":"Understanding how the gut communicates with the brain, via sensory nerves, is of significant interest to medical science. Enteroendocrine cells (EEC) that line the mucosa of the gastrointestinal tract release neurochemicals, including the largest quantity of 5-hydroxytryptamine (5-HT). How the release of substances, like 5-HT, from enterochromaffin (EC) cells activates vagal afferent nerve endings is unresolved. We performed anterograde labelling from nodose ganglia in vivo and identified vagal afferent axons and nerve endings in the mucosa of whole-mount full-length preparations of mouse colon. We then determined the spatial relationship between mucosal-projecting vagal afferent nerve endings and EC cells in situ using 3D imaging. The mean distances between vagal afferent nerve endings in the mucosa, or nearest varicosities along vagal afferent axon branches, and the nearest EC cell were 29.6 ± 19.2 μm (n = 107, N = 6) and 25.7 ± 15.2 μm (n = 119, N = 6), respectively. No vagal afferent endings made close contacts with EC cells. The distances between EC cells and vagal afferent endings are many hundreds of times greater than known distances between pre- and post-synaptic membranes (typically 10-20 nm) that underlie synaptic transmission in vertebrates. The absence of any close physical contacts between 5-HT-containing EC cells and vagal afferent nerve endings in the mucosa leads to the inescapable conclusion that the mechanism by which 5-HT release from ECs in the colonic mucosa occurs in a paracrine fashion, to activate vagal afferents.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"313-327"},"PeriodicalIF":3.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11144134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges of mesenchymal stem cells in the clinical treatment of COVID-19. 间充质干细胞在 COVID-19 临床治疗中面临的挑战。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-06-01 Epub Date: 2024-03-21 DOI: 10.1007/s00441-024-03881-y

Luping Li, Xiaoshuang Zhang, Yawen Wu, Cencan Xing, Hongwu Du

{"title":"Challenges of mesenchymal stem cells in the clinical treatment of COVID-19.","authors":"Luping Li, Xiaoshuang Zhang, Yawen Wu, Cencan Xing, Hongwu Du","doi":"10.1007/s00441-024-03881-y","DOIUrl":"10.1007/s00441-024-03881-y","url":null,"abstract":"The 2019 coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has brought an enormous public health burden to the global society. The duration of the epidemic, the number of infected people, and the widespread of the epidemic are extremely rare in modern society. In the initial stage of infection, people generally show fever, cough, and dyspnea, which can lead to pneumonia, acute respiratory syndrome, kidney failure, and even death in severe cases. The strong infectivity and pathogenicity of SARS-CoV-2 make it more urgent to find an effective treatment. Mesenchymal stem cells (MSCs) are a kind of pluripotent stem cells with the potential for self-renewal and multi-directional differentiation. They are widely used in clinical experiments because of their low immunogenicity and immunomodulatory function. Mesenchymal stem cell-derived exosomes (MSC-Exo) can play a physiological role similar to that of stem cells. Since the COVID-19 pandemic, a series of clinical trials based on MSC therapy have been carried out. The results show that MSCs are safe and can significantly improve patients' respiratory function and prognosis of COVID-19. Here, the effects of MSCs and MSC-Exo in the treatment of COVID-19 are reviewed, and the clinical challenges that may be faced in the future are clarified.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"293-312"},"PeriodicalIF":3.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood-testis barrier: a review on regulators in maintaining cell junction integrity between Sertoli cells. 血液-睾丸屏障：关于维持 Sertoli 细胞间细胞连接完整性的调节因子的综述。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-04-02 DOI: 10.1007/s00441-024-03894-7

Uddesh Ramesh Wanjari, Abilash Valsala Gopalakrishnan

{"title":"Blood-testis barrier: a review on regulators in maintaining cell junction integrity between Sertoli cells.","authors":"Uddesh Ramesh Wanjari, Abilash Valsala Gopalakrishnan","doi":"10.1007/s00441-024-03894-7","DOIUrl":"10.1007/s00441-024-03894-7","url":null,"abstract":"The blood-testis barrier (BTB) is formed adjacent to the seminiferous basement membrane. It is a distinct ultrastructure, partitioning testicular seminiferous epithelium into apical (adluminal) and basal compartments. It plays a vital role in developing and maturing spermatocytes into spermatozoa via reorganizing its structure. This enables the transportation of preleptotene spermatocytes across the BTB, from basal to adluminal compartments in the seminiferous tubules. Several bioactive peptides and biomolecules secreted by testicular cells regulate the BTB function and support spermatogenesis. These peptides activate various downstream signaling proteins and can also be the target themself, which could improve the diffusion of drugs across the BTB. The gap junction (GJ) and its coexisting junctions at the BTB maintain the immunological barrier integrity and can be the \"gateway\" during spermatocyte transition. These junctions are the possible route for toxicant entry, causing male reproductive dysfunction. Herein, we summarize the detailed mechanism of all the regulators playing an essential role in the maintenance of the BTB, which will help researchers to understand and find targets for drug delivery inside the testis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"157-175"},"PeriodicalIF":3.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140334833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C-C motif chemokine ligand 2 regulates prostaglandin synthesis and embryo attachment of the bovine endometrium during implantation. C-C motif趋化因子配体 2 调节前列腺素合成和牛子宫内膜植入过程中的胚胎附着。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-03-05 DOI: 10.1007/s00441-024-03869-8

Chi Sun Yun, Yuyu Saito, Al-Nur Md Iftekhar Rahman, Takahiro Suzuki, Hideyuki Takahashi, Keiichiro Kizaki, M A M Yahia Khandoker, Nobuhiko Yamauchi

{"title":"C-C motif chemokine ligand 2 regulates prostaglandin synthesis and embryo attachment of the bovine endometrium during implantation.","authors":"Chi Sun Yun, Yuyu Saito, Al-Nur Md Iftekhar Rahman, Takahiro Suzuki, Hideyuki Takahashi, Keiichiro Kizaki, M A M Yahia Khandoker, Nobuhiko Yamauchi","doi":"10.1007/s00441-024-03869-8","DOIUrl":"10.1007/s00441-024-03869-8","url":null,"abstract":"C-C motif chemokine ligand 2 (CCL2) has been reported to be expressed in the bovine endometrium during pregnancy. However, the details of its functions involved in the implantation mechanism are still not clear. The purpose of this study is to analyze the functional properties of CCL2 in the bovine endometrium and embryos. The expression of CCR2 was not different between the luteal phase and implantation phase of their endometrial tissues, but was significantly high in IFNa treated bovine endometrial stromal (BES) cells in vitro. The expressions of PGES1, PGES2, AKR1C4, and AKR1C4 were high at the implantation stage compared with the luteal stage. On the other hand, PGES2 and AKR1B1 in BEE and PGES3 and AKR1A1 in BES were significantly increased by CCL2 treatment, respectively. The expressions of PCNA and IFNt were found significantly high in the bovine trophoblastic cells (BT) treated with CCL2 compared to the control. CCL2 significantly increased the attachment rate of BT vesicles to BEE in in vitro co-culture system. The expression of OPN and ICAM-1 increased in BEE, and ICAM-1 increased in BT by CCL2 treatment, respectively. The present results indicate that CCL2 has the potential to regulate the synthesis of PGs in the endometrium and the embryo growth. In addition, CCL2 has the possibility to regulate the process of bovine embryo attachment to the endometrium by modulation of binding molecules expression.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"231-243"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140027502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Telocytes: current methods of research, challenges and future perspectives. Telocytes: 当前的研究方法、挑战和未来展望。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-03-28 DOI: 10.1007/s00441-024-03888-5

Bruno D A Sanches, Francisco B S Teófilo, Mathieu Y Brunet, Victor M Villapun, Kenny Man, Lara C Rocha, Jurandyr Pimentel Neto, Marta R Matsumoto, Juliana S Maldarine, Adriano P Ciena, Sophie C Cox, Hernandes F Carvalho

{"title":"Telocytes: current methods of research, challenges and future perspectives.","authors":"Bruno D A Sanches, Francisco B S Teófilo, Mathieu Y Brunet, Victor M Villapun, Kenny Man, Lara C Rocha, Jurandyr Pimentel Neto, Marta R Matsumoto, Juliana S Maldarine, Adriano P Ciena, Sophie C Cox, Hernandes F Carvalho","doi":"10.1007/s00441-024-03888-5","DOIUrl":"10.1007/s00441-024-03888-5","url":null,"abstract":"Telocytes (TCs) are CD34-positive interstitial cells that have long cytoplasmic projections, called telopodes; they have been identified in several organs and in various species. These cells establish a complex communication network between different stromal and epithelial cell types, and there is growing evidence that they play a key role in physiology and pathology. In many tissues, TC network impairment has been implicated in the onset and progression of pathological conditions, which makes the study of TCs of great interest for the development of novel therapies. In this review, we summarise the main methods involved in the characterisation of these cells as well as their inherent difficulties and then discuss the functional assays that are used to uncover the role of TCs in normal and pathological conditions, from the most traditional to the most recent. Furthermore, we provide future perspectives in the study of TCs, especially regarding the establishment of more precise markers, commercial lineages and means for drug delivery and genetic editing that directly target TCs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"141-155"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: BMP2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells. 更正为BMP2 可诱导干细胞的软骨分化、成骨分化和软骨内骨化。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 DOI: 10.1007/s00441-024-03882-x

Nian Zhou, Qi Li, Xin Lin, Ning Hu, Jun-Yi Liao, Liang-Bo Lin, Chen Zhao, Zhen-Ming Hu, Xi Liang, Wei Xu, Hong Chen, Wei Huang

引用次数: 0

Regional differences in the ultrastructure of mucosal macrophages in the rat large intestine. 大鼠大肠粘膜巨噬细胞超微结构的区域差异。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-03-15 DOI: 10.1007/s00441-024-03883-w

Shota Murase, Youhei Mantani, Nobuhiko Ohno, Asaka Shimada, Satoki Nakanishi, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi

{"title":"Regional differences in the ultrastructure of mucosal macrophages in the rat large intestine.","authors":"Shota Murase, Youhei Mantani, Nobuhiko Ohno, Asaka Shimada, Satoki Nakanishi, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi","doi":"10.1007/s00441-024-03883-w","DOIUrl":"10.1007/s00441-024-03883-w","url":null,"abstract":"We previously clarified the histological characteristics of macrophages in the rat small intestine using serial block-face scanning electron microscopy (SBF-SEM). However, the regional differences in the characteristics of macrophages throughout the large intestine remain unknown. Here, we performed a pilot study to explore the regional differences in the ultrastructure of mucosal macrophages in the large intestine by using SBF-SEM analysis. SBF-SEM analysis conducted on the luminal side of the cecum and descending colon revealed macrophages as amorphous cells possessing abundant lysosomes and vacuoles. Macrophages in the cecum exhibited a higher abundance of lysosomes and a lower abundance of vacuoles than those in the descending colon. Macrophages with many intraepithelial cellular processes were observed beneath the intestinal superficial epithelium in the descending colon. Moreover, macrophages in contact with nerve fibers were more prevalent in the cecum than in the descending colon, and a subset of them surrounded a nerve bundle only in the cecum. In conclusion, the present pilot study suggested that the quantity of some organelles (lysosomes and vacuoles) in macrophages differed between the cecum and the descending colon and that there were some region-specific subsets of macrophages like nerve-associated macrophages in the cecum.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"245-253"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140130828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic relationship among extracellular matrix and body wall cells in Hirudo verbana morphogenesis. 细胞外基质和体壁细胞在马鞭草形态发生中的动态关系

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-03-01 DOI: 10.1007/s00441-024-03874-x

Laura Pulze, Nicolò Baranzini, Francesco Acquati, Gaia Marcolli, Annalisa Grimaldi

{"title":"Dynamic relationship among extracellular matrix and body wall cells in Hirudo verbana morphogenesis.","authors":"Laura Pulze, Nicolò Baranzini, Francesco Acquati, Gaia Marcolli, Annalisa Grimaldi","doi":"10.1007/s00441-024-03874-x","DOIUrl":"10.1007/s00441-024-03874-x","url":null,"abstract":"A great bulk of recent experimental evidence suggests the key role of the complex crosstalk between the extracellular matrix (ECM) and the cellular component of tissues during morphogenesis and embryogenesis. In particular, remodeling of the ECM and of its physical interactions pattern with surrounding cells represent two crucial processes that might be involved in muscle development. However, little information is available on this topic, especially on invertebrate species. To obtain new insights on how tuning the ECM microenvironment might drive cellular fate during embryonic development, we used the invertebrate medicinal leech Hirudo verbana as a valuable experimental model, due to its simple anatomy and the recapitulation of many aspects of the basic biological processes of vertebrates. Our previous studies on leech post-embryonic development have already shown the pivotal role of ECM changes during the growth of the body wall and the role of Yes-associated protein 1 (YAP1) in mechanotransduction. Here, we suggest that the interactions between stromal cell telocytes and ECM might be crucial in driving the organization of muscle layers during embryogenesis. Furthermore, we propose a possible role of the pleiotropic enzyme HvRNASET2 as a possible modulator of collagen deposition and ECM remodeling not only during regenerative processes (as previously demonstrated) but also in embryogenesis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"213-229"},"PeriodicalIF":3.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11055932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entry of cannabidiol into the fetal, postnatal and adult rat brain. 大麻二酚进入胎儿、出生后和成年大鼠大脑的情况。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-02-17 DOI: 10.1007/s00441-024-03867-w

Georgia Fitzpatrick, Yifan Huang, Fiona Qiu, Mark D Habgood, Robert L Medcalf, Heidi Ho, Katarzyna M Dziegielewska, Norman R Saunders

{"title":"Entry of cannabidiol into the fetal, postnatal and adult rat brain.","authors":"Georgia Fitzpatrick, Yifan Huang, Fiona Qiu, Mark D Habgood, Robert L Medcalf, Heidi Ho, Katarzyna M Dziegielewska, Norman R Saunders","doi":"10.1007/s00441-024-03867-w","DOIUrl":"10.1007/s00441-024-03867-w","url":null,"abstract":"Cannabidiol is a major component of cannabis but without known psychoactive properties. A wide range of properties have been attributed to it, such as anti-inflammatory, analgesic, anti-cancer, anti-seizure and anxiolytic. However, being a fairly new compound in its purified form, little is known about cannabidiol brain entry, especially during development. Sprague Dawley rats at four developmental ages: embryonic day E19, postnatal day P4 and P12 and non-pregnant adult females were administered intraperitoneal cannabidiol at 10 mg/kg with [3H] labelled cannabidiol. To investigate the extent of placental transfer, the drug was injected intravenously into E19 pregnant dams. Levels of [3H]-cannabidiol in blood plasma, cerebrospinal fluid and brain were estimated by liquid scintillation counting. Plasma protein binding of cannabidiol was identified by polyacrylamide gel electrophoresis and its bound and unbound fractions measured by ultrafiltration. Using available RNA-sequencing datasets of E19 rat brain, choroid plexus and placenta, as well as P5 and adult brain and choroid plexus, expression of 13 main cannabidiol receptors was analysed. Results showed that cannabidiol rapidly entered both the developing and adult brains. Entry into CSF was more limited. Its transfer across the placenta was substantially restricted as only about 50% of maternal blood plasma cannabidiol concentration was detected in fetal plasma. Albumin was the main, but not exclusive, cannabidiol binding protein at all ages. Several transcripts for cannabidiol receptors were expressed in age- and tissue-specific manner indicating that cannabidiol may have different functional effects in the fetal compared to adult brain.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"177-195"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11055756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139746240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracrine and endocrine pathways of natriuretic peptides assessed by ligand-receptor mapping in the Japanese eel brain. 通过配体-受体映射评估日本鳗鱼大脑中钠利尿肽的旁分泌和内分泌途径。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-02-19 DOI: 10.1007/s00441-024-03873-y

Tomoki Izumi, Ami Saito, Takanori Ida, Takao Mukuda, Yukitoshi Katayama, Marty Kwok-Shing Wong, Takehiro Tsukada

{"title":"Paracrine and endocrine pathways of natriuretic peptides assessed by ligand-receptor mapping in the Japanese eel brain.","authors":"Tomoki Izumi, Ami Saito, Takanori Ida, Takao Mukuda, Yukitoshi Katayama, Marty Kwok-Shing Wong, Takehiro Tsukada","doi":"10.1007/s00441-024-03873-y","DOIUrl":"10.1007/s00441-024-03873-y","url":null,"abstract":"The natriuretic peptide (NP) family consists of cardiac NPs (ANP, BNP, and VNP) and brain NPs (CNPs) in teleosts. In addition to CNP1-4, a paralogue of CNP4 (named CNP4b) was recently discovered in basal teleosts including Japanese eel. Mammals have lost most Cnps during the evolution, but teleost cnps were conserved and diversified, suggesting that CNPs are important hormones for maintaining brain functions in teleost. The present study evaluated the potency of each Japanese eel CNP to their NP receptors (NPR-A, NPR-B, NPR-C, and NPR-D) overexpressed in CHO cells. A comprehensive brain map of cnps- and nprs-expressing neurons in Japanese eel was constructed by integrating the localization results obtained by in situ hybridization. The result showed that CHO cells expressing NPR-A and NPR-B induced strong cGMP productions after stimulation by cardiac and brain NPs, respectively. Regarding brain distribution of cnps, cnp1 is engaged in the ventral telencephalic area and periventricular area including the parvocellular preoptic nucleus (Pp), anterior/posterior tuberal nuclei, and periventricular gray zone of the optic tectum. cnp3 is found in the habenular nucleus and prolactin cells in the pituitary. cnp4 is expressed in the ventral telencephalic area, while cnp4b is expressed in the motoneurons in the medullary area. Such CNP isoform-specific localizations suggest that function of each CNP has diverged in the eel brain. Furthermore, the Pp lacking the blood-brain barrier expressed both npra and nprb, suggesting that endocrine and paracrine NPs interplay for regulating the Pp functions in Japanese eels.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"197-212"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0