Cell and Tissue Research最新文献_第7页

Pannexins in the heart: cell-specific expression and contributions to disease. 心脏泛联蛋白：细胞特异性表达和对疾病的贡献。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-22 DOI: 10.1007/s00441-026-04046-9

Mark C Renton, Meghan W Sedovy, Amanda Reynolds, Adam Hoch, Kailynn Roberts, Renée Sarmiento, Caroline Toler, Scott R Johnstone

{"title":"Pannexins in the heart: cell-specific expression and contributions to disease.","authors":"Mark C Renton, Meghan W Sedovy, Amanda Reynolds, Adam Hoch, Kailynn Roberts, Renée Sarmiento, Caroline Toler, Scott R Johnstone","doi":"10.1007/s00441-026-04046-9","DOIUrl":"10.1007/s00441-026-04046-9","url":null,"abstract":"Heart disease is the leading cause of death globally. Although modern interventions have dramatically reduced the morbidity and mortality of heart disease, the lack of knowledge of key underlying mechanisms has limited the development of effective therapeutics. Pannexins encompass a group of three transmembrane channel-forming proteins best known for their role in purinergic signaling through the release of ATP. Pannexins, particularly pannexin 1 (Panx1), are expressed in multiple cell types throughout the heart and play a role in blood vessel regulation, immune cell recruitment and activation, and the response to ischemic injury. In this review, we analyze publicly available sequencing data to investigate the expression of pannexin proteins in human and mouse hearts at both tissue and single-cell levels. We provide a detailed review of the literature surrounding cardiac pannexin function in the context of both ischemic and non-ischemic heart disease. We then discuss the clinical use of drugs now known to target pannexin channels as a primer for the therapeutic potential of pannexins in cardiac dysfunction. Finally, we discuss the largest gaps in the current literature to guide future research.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"11"},"PeriodicalIF":3.1,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12827358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autonomic neural control of motor activity in the intestine of freshwater barramundi (Lates calcarifer). 淡水barramundi （calcarifer）肠道运动活动的自主神经控制。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-20 DOI: 10.1007/s00441-025-04034-5

Hayley Rhodes, Lee Travis, Timothy J Hibberd, Nick J Spencer, James Harris

{"title":"Autonomic neural control of motor activity in the intestine of freshwater barramundi (Lates calcarifer).","authors":"Hayley Rhodes, Lee Travis, Timothy J Hibberd, Nick J Spencer, James Harris","doi":"10.1007/s00441-025-04034-5","DOIUrl":"10.1007/s00441-025-04034-5","url":null,"abstract":"Evolutionary pressure on the gastrointestinal (GI) tract of teleost fish differs substantially from those of terrestrial animals. The intestine is a pivotal organ in teleost, for maintaining osmotic balance against the surrounding aquatic environment; primarily through its role in water absorption. Intestinal sensory and motor functions of vertebrates are largely mediated by the enteric nervous system (ENS) embedded within the gut wall. Although the ENS has been described in several teleost species, the group comprised of more than 25,000 species displays remarkable ecological, anatomical, and physiological diversity. As such, species that display distinct adaptations, i.e., euryhalinity, may provide valuable comparative insights. Here we show the structure of the ENS within the intestine of barramundi (Lates calcarifer), a catadromous perch species with a unique life history and growing commercial relevance. Immunohistochemical labelling identified enteric neurons synthesising nitric oxide synthase (NOS) and calcitonin-gene-related peptide (CGRP), both of which were typically uniaxonal with smooth cell bodies. Qualitatively, these neuronal populations formed a weakly arranged enteric plexus, analogous to the myenteric plexus found in terrestrial animals. Quantitatively, the proportion of NOS immunoreactive neurons decreased along the rostro-caudal axis of the intestine without accompanying changes to the overall neuronal density. Video imaging of intestinal wall movements ex vivo identified multiple recurrent motility patterns which were hexamethonium-sensitive, suggesting regulation by nicotinic synaptic transmission within enteric pathways. Together, these findings show that enteric neurons are present in the barramundi intestine but form a comparatively less defined plexus than in higher terrestrial vertebrates. These enteric neurons are involved in the regulation of intestinal motility via nicotinic transmission.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"10"},"PeriodicalIF":3.1,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12819503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meckel's cartilage midsegment: spatiotemporal dynamics and osteogenic role in mice. 小鼠梅克尔软骨中段：时空动力学和成骨作用。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-17 DOI: 10.1007/s00441-025-04039-0

Qi Sun, Cheng Li, Lizhu Yin, Chuxuan Xiao, Yi Peng, Juan Liu, Jun Zhang

{"title":"Meckel's cartilage midsegment: spatiotemporal dynamics and osteogenic role in mice.","authors":"Qi Sun, Cheng Li, Lizhu Yin, Chuxuan Xiao, Yi Peng, Juan Liu, Jun Zhang","doi":"10.1007/s00441-025-04039-0","DOIUrl":"10.1007/s00441-025-04039-0","url":null,"abstract":"Meckel's cartilage is a transient yet essential structure in the development of the mammalian mandible, with its midsegment undergoing distinct degradation and potentially contributing to bone formation. However, the mechanisms regulating its fate remain unclear. This study aimed to investigate the spatiotemporal changes and potential osteogenic function of the midsegment of Meckel's cartilage during mouse mandibular development. Using C57BL/6 J mice and a tamoxifen-inducible cartilage-specific lineage tracing model, we conducted morphological staining (Alcian Blue, Safranin O, Masson's trichrome) and immunofluorescence analyses targeting key transcription factors (Sox9, Runx2, Osterix) and vascular marker CD31 from embryonic day 12.5 to postnatal day 0. The anterior portion of the midsegment displayed endochondral-like ossification, marked by chondrocyte hypertrophy, matrix remodeling, and subsequent osteogenic invasion. A shift from cell proliferation to differentiation was noted as Ki67-positive cells decreased by embryonic day 14.5. Sequential expression of Sox9, Runx2, and Osterix was observed along the chondrocyte maturation axis, with peripheral localization of Osterix-expressing cells in remodeling zones. Endothelial cells expressing CD31 were closely associated with osteoprogenitor cells co-expressing Runx2 or Osterix, particularly on the incisor-facing side, indicating a spatial relationship between vascular structures and osteogenic differentiation. These findings demonstrate that the midsegment of Meckel's cartilage undergoes an endochondral-like ossification process similar to long bones, involving a Sox9 → Runx2 → Osterix transcriptional cascade and vascular invasion. Thus, our study provides novel evidence of the dynamic ossification of Meckel's cartilage midsegment, clarifying its role in mandibular development and contributing to the long-standing debate on its evolutionary and developmental significance.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"8"},"PeriodicalIF":3.1,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12811375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145988342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organelles with screening pigment in the supporting cells of the retina of the camera-like eye of the gastropod Helix pomatia. 腹足动物照相机状眼视网膜支持细胞中具有筛选色素的细胞器。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-17 DOI: 10.1007/s00441-025-04032-7

Irina P Shepeleva

引用次数: 0

Possible functional origin of TPO-like genes in chordates revealed by their pleiotropic expression and disturbed phenomena in protochordates. 原脊索动物中tpo样基因的多向性表达和紊乱现象揭示了其在脊索动物中可能的功能起源。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-16 DOI: 10.1007/s00441-025-04035-4

Hiroki Kawamorita, Haruka Fujiwara, Keito Fujita, Akiko Hozumi, Sumio Udagawa, Ryuta Yoshida, Masato Kiyomoto, Yasunori Sasakura, Michio Ogasawara

{"title":"Possible functional origin of TPO-like genes in chordates revealed by their pleiotropic expression and disturbed phenomena in protochordates.","authors":"Hiroki Kawamorita, Haruka Fujiwara, Keito Fujita, Akiko Hozumi, Sumio Udagawa, Ryuta Yoshida, Masato Kiyomoto, Yasunori Sasakura, Michio Ogasawara","doi":"10.1007/s00441-025-04035-4","DOIUrl":"10.1007/s00441-025-04035-4","url":null,"abstract":"Mammalian thyroid peroxidase (TPO), a thyroid-specific peroxidase involved in thyroid hormone synthesis, has a characteristic hydrophobic region in the C-terminus (HRC) that anchors peroxidase to the apical membrane of epithelial follicle cells. Protochordates (lancelets and ascidians) have TPO-like (TPOL) peroxidases with or without HRC, and their expression has been reported in the endostyle. We herein investigated the molecular structures of TPOL in protochordates and TPO relatives in vertebrates, including eosinophil peroxidase, myeloperoxidase, and lactoperoxidase, with a focus on the existence of HRC. Our molecular phylogenetic analysis implied that ancestral chordates evolved HRC-containing peroxidases, and mammalian TPO maintained HRC despite many TPO relatives losing it. Gene expression profiles revealed by in situ hybridization and an RNA-seq analysis showed that transcripts of TPOL genes in protochordates were distributed to pharyngeal epithelia in addition to the endostyle. Furthermore, disturbances in TPO activity by a thiourea treatment and gene knockdown experiments resulted in a deficiency of pharyngeal mucus-sheets. Collectively, these results suggest that HRC-containing TPOL was originally involved in pharyngeal mucus-sheet formation for suspension feeding in ancestral chordates, and HRC may have evolved to maintain peroxidase anchoring to the apical surface of pharyngeal epithelia.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"7"},"PeriodicalIF":3.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145988475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Panx1 and drug discovery: from non-specific inhibitors to tailored drug compounds. Panx1和药物发现：从非特异性抑制剂到定制药物化合物。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-16 DOI: 10.1007/s00441-025-04038-1

Michael Kohr, Carola Meier

引用次数: 0

Characterization of bovine oviductal organoids: Polarity, cryopreservation, hormonal stimulation, and extracellular vesicles. 牛输卵管类器官的特征：极性、冷冻保存、激素刺激和细胞外囊泡。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-16 DOI: 10.1007/s00441-025-04036-3

Riley E Thompson-Brandhagen, Mindy A Meyers, Brandi Dunn, Nico G Menjivar, Jennifer Palmer, D N Rao Veeramachaneni, Dawit Tesfaye, Fiona K Hollinshead

{"title":"Characterization of bovine oviductal organoids: Polarity, cryopreservation, hormonal stimulation, and extracellular vesicles.","authors":"Riley E Thompson-Brandhagen, Mindy A Meyers, Brandi Dunn, Nico G Menjivar, Jennifer Palmer, D N Rao Veeramachaneni, Dawit Tesfaye, Fiona K Hollinshead","doi":"10.1007/s00441-025-04036-3","DOIUrl":"10.1007/s00441-025-04036-3","url":null,"abstract":"The oviduct is an important reproductive organ that fosters fertilization and early embryo development in mammals. With growing utilization of assisted reproductive technologies (ARTs) in animal agriculture, recent advancements in cell culture modeling have the potential to optimize in vitro fertilization methods. Organoids are a three-dimensional (3D) cell culture model poised to serve a key role in our understanding of endogenous oviductal physiology in cattle. However, limited information is currently available for the use of bovine oviductal organoids. Thus, the objective of our study was to conduct a comprehensive characterization of bovine oviductal organoids including polarity, cryopreservation, responsiveness to hormonal cues, and extracellular vesicle assessment. We observed that bovine oviductal organoids have the capacity for culture longevity for at least 86 days with or without cryopreservation. Furthermore, the organoid polarity can be manipulated by presence or absence of an extracellular matrix, and they respond to stimulation with hormones associated with estrus and diestrus through gene expression changes and extracellular vesicle secretion. In conclusion, bovine organoids display similar characteristics to in vivo oviductal cells and constitute a cell culture model in which to study oviductal physiology to improve ARTs in beef and dairy agriculture.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"5"},"PeriodicalIF":3.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145988359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Connexin-Pannexin duality in glioblastoma. 胶质母细胞瘤中的连接蛋白-泛连接蛋白二象性。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-16 DOI: 10.1007/s00441-025-04031-8

Rehanna Kanji, Silvia Penuela, Marc Mesnil

{"title":"Connexin-Pannexin duality in glioblastoma.","authors":"Rehanna Kanji, Silvia Penuela, Marc Mesnil","doi":"10.1007/s00441-025-04031-8","DOIUrl":"10.1007/s00441-025-04031-8","url":null,"abstract":"Glioblastoma (GBM) is the most aggressive primary brain tumour in adults, characterized by extensive heterogeneity and diffuse invasion. Increasing evidence highlights the role of channel-forming proteins in glioma biology. Connexins (Cxs) and Pannexins (Panxs) are two structurally related yet functionally distinct protein families that mediate cellular communication. While both regulate overlapping processes such as ion homeostasis and ATP release, only Cxs are capable of readily forming intercellular gap junctions. Cx43 is the most extensively studied connexin in GBM and seems to display paradoxical roles, acting as a tumour suppressor by reducing proliferation while promoting invasion. In contrast, Panx1 predominantly supports tumour progression, while Panx2 exerts tumour-suppressive effects. This review synthesizes how Cxs and Panxs exert context-dependent and sometimes opposing effects across stages of GBM, including proliferation and invasion, and examines how divergent experimental models may contribute to apparent contradictions. Discrepancies among studies often arise from differences in model systems, which may not recapitulate the complexity of human GBM. Given the profound heterogeneity of this tumour, future research should prioritize patient-derived and three-dimensional models that more accurately represent the human disease context. Such approaches will be essential to clarify the context-dependent functions of Cxs and Panxs and their potential as therapeutic targets in GBM.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"4"},"PeriodicalIF":3.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145988372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aldosterone induced by chronic stress promotes pancreatic steatosis in young male rats. 慢性应激诱导的醛固酮促进年轻雄性大鼠胰腺脂肪变性。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-16 DOI: 10.1007/s00441-025-04029-2

Eliut Pérez-Sánchez, Cristhian Neftaly Sánchez-Solís, Sumiko Morimoto, Adriana Corona-Pérez, Margarita Martínez-Gómez, Jorge Rodríguez-Antolín, Estela Cuevas-Romero, Leticia Nicolás-Toledo

{"title":"Aldosterone induced by chronic stress promotes pancreatic steatosis in young male rats.","authors":"Eliut Pérez-Sánchez, Cristhian Neftaly Sánchez-Solís, Sumiko Morimoto, Adriana Corona-Pérez, Margarita Martínez-Gómez, Jorge Rodríguez-Antolín, Estela Cuevas-Romero, Leticia Nicolás-Toledo","doi":"10.1007/s00441-025-04029-2","DOIUrl":"10.1007/s00441-025-04029-2","url":null,"abstract":"Aldosterone, a stress-related hormone, may contribute to pancreatic dysfunction. Its role in pancreatic physiology, inflammation, and fibrosis remains poorly understood. This study aimed to evaluate the effects of aldosterone on pancreatic histomorphology and islet cellularity under chronic stress conditions, and to determine whether eplerenone, a selective aldosterone receptor antagonist, can counteract them. Twenty-four young male Wistar rats were randomly divided into four groups (n = 6): control, chronic unpredictable mild stress (CUMS), control + vehicle (control + Veh), and CUMS + eplerenone (EP). Eplerenone was administered on postnatal day 51 via gastric tube 2 h before daily stress exposure for 4 weeks. Histological, immunohistochemical, and biochemical analyses were performed, including quantification of aldosterone, visceral adipose tissue, and pancreatic triacylglycerol. In CUMS group, the aldosterone level increased. In the exocrine pancreas, the aldosterone increased intralobular fat and triacylglycerol accumulation. Tumor necrosis factor alpha (TNFα)-positive necrotic acinar cells with pyknotic nuclei and perivascular fibrosis were evident. The endocrine pancreas exhibited increased inflammation and an altered distribution of islet sizes. Eplerenone reduced fat accumulation, inflammation, and exocrine structural damage but did not prevent TNF-α expression in islets. In conclusion, aldosterone induced by chronic stress contributes to pancreatic steatosis, and islet toxicity in young male rats, supporting aldosterone's pathogenic role.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"3"},"PeriodicalIF":3.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145988396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructure of the black soldier fly antennal sensilla (Hermetia illucens, Stratiomyidae: Hermetinae). 黑兵蝇触角感受器的超微结构（黑兵蝇科）。

IF 3.1 3区生物学

Cell and Tissue Research Pub Date : 2026-01-15 DOI: 10.1007/s00441-025-04041-6

Silvana Piersanti, Gianandrea Salerno, Mariangela Gentile, Eugenio Paccagnini, Manuela Rebora

{"title":"Ultrastructure of the black soldier fly antennal sensilla (Hermetia illucens, Stratiomyidae: Hermetinae).","authors":"Silvana Piersanti, Gianandrea Salerno, Mariangela Gentile, Eugenio Paccagnini, Manuela Rebora","doi":"10.1007/s00441-025-04041-6","DOIUrl":"10.1007/s00441-025-04041-6","url":null,"abstract":"Every year, more than two hundred billion individuals of the black soldier fly Hermetia illucens are reared on an industrial scale to convert a wide range of organic by-products into high-value materials for agricultural and industrial applications. The present ultrastructural investigation, using scanning (SEM) and transmission (TEM) electron microscopy, describes the fine morphology of antennal sensilla in males and females: three types of olfactory sensilla (two multiporous and one coeloconic), one type of uniporous grooved sensillum chaeticum with gustatory and mechanosensory functions, and one type of small peg sensillum with a probable hygroreceptive function. In addition, pseudo-lamellate sensory neurons, not connected to the cuticle, are described in the last two flagellar segments, which otherwise lack sensilla. These findings provide essential groundwork for future electrophysiological and behavioral investigations to deepen the knowledge on H. illucens sensory biology, which may contribute to optimizing global mass-rearing systems. Moreover, the results, together with hypotheses on the function of the paddle-like last flagellomere, may be relevant for exploring the evolutionary differentiation of the ancestral Dipteran flagellum into the complex sensory system characteristic of the Brachycera.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"2"},"PeriodicalIF":3.1,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145970621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0