Cell and Tissue Research最新文献

{"title":"Significant expression of prosaposin in the intermediate lobe of mouse pituitary gland.","authors":"Aimi Fuyuki, Masashi Higuchi, Takeshi Homma, Kai Kitamura, Sawa Onouchi, Shouichiro Saito","doi":"10.1007/s00441-026-04054-9","DOIUrl":"10.1007/s00441-026-04054-9","url":null,"abstract":"<p><p>Prosaposin (PSAP) is a highly conserved glycoprotein in vertebrates. It is known to be transported into lysosomes and facilitates lysosomal hydrolysis. In addition, PSAP is secreted in various body fluids, including serum. Extracellular PSAP is known to function as a trophic factor for neurons, and recent studies have revealed that PSAP plays a pivotal role in dopaminergic neuron homeostasis. This study examined PSAP expression in the mouse pituitary gland, which is one of the principal sources of circulating hormones innervated by dopaminergic neurons. In situ hybridization showed that PSAP mRNA expression was high in the intermediate lobe (IL), whereas the expression was relatively low and sparse in the anterior (AL) and posterior lobes (PL). Immunohistochemical analyses showed that PSAP immunoreactivity was detected as fine, granular structures in the AL and IL. PSAP immunoreactivity was also observed in glial cells and the Herring bodies of the PL. The IL is innervated by axons from dopaminergic neurons in the periventricular hypothalamic area, and neurotrophic factors, such as brain-derived neurotrophic factor (BDNF), are known to be highly expressed in the IL, where they support these neurons. The results of this study indicate that PSAP plays a pivotal role in the pituitary gland, particularly within the IL.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"21"},"PeriodicalIF":3.1,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12907278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles at the neuromuscular junction: messengers of synaptic health and disease.","authors":"Rizwan Qaisar","doi":"10.1007/s00441-026-04050-z","DOIUrl":"10.1007/s00441-026-04050-z","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) have emerged as pivotal modulators of neuromuscular junction (NMJ) biology, reshaping our understanding of synaptic communication, maintenance, and degeneration. This review consolidates current insights into the roles of EVs derived from motor neurons, muscle fibers, and Schwann cells in regulating NMJ integrity. In healthy states, EVs deliver trophic factors, structural proteins, and regulatory RNAs that promote the clustering of acetylcholine receptors, presynaptic stability, and axonal growth. Motor neuron EVs carry Wnt7a, synaptophysin, and PGC-1α, while muscle-derived EVs deliver miR-206, agrin, and caveolin-3. Schwann cell EVs contribute neurotrophic support via NRG1 and GDNF. In contrast, diseased or aged NMJs exhibit EV cargo dysregulation, marked by the presence of misfolded proteins (e.g., SOD1, TDP-43), pro-inflammatory cytokines, and reduced regenerative miRNAs. These changes contribute to synaptic dismantling, neuroinflammation, and impaired repair in conditions such as ALS, SMA, MG, and sarcopenia. The review highlights the bidirectional nature of EV signalling and its dynamic regulation by neuronal activity and stress. Emerging therapeutic strategies include engineering EVs to deliver protective cargo, targeting them to NMJ components, and designing biomaterial-based depots for sustained release. Furthermore, EV signatures in blood and muscle hold promise as non-invasive biomarkers for early detection of NMJ decline in ALS, SMA, MG, and sarcopenia. Despite promising preclinical data, challenges remain in EV characterization, targeting specificity, and clinical translation. This review underscores a paradigm shift: EVs are not passive byproducts but active messengers of neuromuscular health and disease, with realistic applications in diagnostics, regenerative therapy, and personalized medicine.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"20"},"PeriodicalIF":2.9,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146178083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuronal nitric oxide synthase expression in secretory cells of the human parotid and submandibular salivary glands: evidence from light and electron microscopy.","authors":"Michela Isola, Marianna Boi, Roberto Demontis, Raffaella Isola, Francesco Loy, Cristina Maxia, Daniela Murtas, Maria Pina Serra, Jörgen Ekström, Marina Quartu","doi":"10.1007/s00441-026-04048-7","DOIUrl":"10.1007/s00441-026-04048-7","url":null,"abstract":"<p><p>Nitric oxide plays a crucial role in multiple physiological processes in salivary glands, yet its precise cellular sources in human glandular tissues remain insufficiently characterized. This study investigated the distribution and subcellular localization of neuronal nitric oxide synthase (nNOS) in human parotid and submandibular gland tissue samples obtained from surgical biopsies and autopsies, using both light and electron-microscope immunohistochemistry. In both gland types, nNOS was consistently observed in serous secretory cells and the ductal epithelium, whereas mucous cells showed no immunoreactivity. Ductal cell staining was heterogeneous, with some cells displaying intense labeling and others remaining unstained. Nerve fibers containing the enzyme were rare within the gland parenchyma. Ultrastructural analysis revealed the enzyme within secretory granules and small cytoplasmic vesicles of serous cells, as well as in the apical and basal cytoplasm, mitochondria, and nuclei of ductal epithelial cells. These findings provide the first ultrastructural evidence of nNOS in secretory and ductal cells of human major salivary glands. The selective localization in serous cells, absence in mucous cells, and limited nitrergic innervation suggest a substantial parenchymal contribution to nitric oxide production. This distribution indicates that nitric oxide regulates saliva formation, composition, and intracellular signaling and could play a broader role in oral physiology and disease.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"19"},"PeriodicalIF":3.1,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146178045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of antibiotics encapsulated in hyaluronic acid hydrogels on different osteogenic cells and bacteria.","authors":"Dimitrios Argyrakis, Ganesh N Nawale, Oommen P Varghese, Evangelos Mourkas, Josef D Järhult, Nils P Hailer, Nikos Schizas","doi":"10.1007/s00441-026-04053-w","DOIUrl":"10.1007/s00441-026-04053-w","url":null,"abstract":"<p><p>Topical application of antibiotics in the treatment of orthopaedic implant-related infections can be achieved by using hyaluronic acid (HA)-based hydrogels as carriers. Our aim was to investigate potential toxic effects of a novel antibiotic-loaded hydrogel on osteogenic cells and its antibacterial effect against staphylococci. A covalently cross-linked hyaluronic acid (HA)-based hydrogel was loaded with increasing concentrations of cefuroxime and vancomycin and their release was examined by UV spectrometry. Primary human (HoBs), mouse (MoBs) osteoblasts, or SaoS-2 cells were either exposed to the drug-loaded hydrogel or to antibiotics alone, followed by assessment of cell metabolism and proliferation. Antibacterial effects were evaluated against Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis). Increasing concentrations of antibiotics did not affect cell metabolism in any osteogenic cell type, whereas cell proliferation remained unaltered in MoBs, was significantly reduced in SaoS-2, and was stimulated in HoBs. Cultures of MoBs and HoBs tolerated higher concentrations of vancomycin than SaoS-2. Antibiotic-loaded hydrogels did not exert toxic effects on HoBs. After 24 h, 16.8% of vancomycin and 70.8% of cefuroxime were released from the hydrogel. Cefuroxime-loaded hydrogels significantly inhibited growth of S. aureus but not of S. epidermidis, while vancomycin-loaded hydrogel had scarce effects on S. epidermidis. Loading HA-based hydrogel with antibiotics does not harm osteoblasts at clinically relevant concentrations but inhibits bacterial growth. Higher loading of vancomycin may be required due to its slow release while cefuroxime is released more rapidly. A resorbable, antibiotic-loaded hydrogel may be used for implant-related infections in orthopaedics.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"18"},"PeriodicalIF":3.1,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12894147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146156297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview in tendon's physiology, pathomorphology, and treatment options.","authors":"Markus Jaschke, Lukas Kolodziej, Chiara Huebotter, Thilo Hennecke, David Koppe, Aleksandra Wilk","doi":"10.1007/s00441-025-04042-5","DOIUrl":"10.1007/s00441-025-04042-5","url":null,"abstract":"<p><p>Tendon degeneration and tendinopathy are common musculoskeletal disorders characterized by chronic pain, impaired function, and structural changes within the tendon. A cascade of pathological changes appears due to several risk factors such as ageing, overuse, obesity, or specific medications. Among these changes are collagen disorganization, neovascularization, altered extracellular matrix composition, and changes in cellular components. Recent studies have highlighted the role of tenocyte apoptosis, matrix metalloproteinase activity, and mechanical stress in the progression of tendinopathy. Biomechanically, a tendon is under elastic and plastic changes in higher amounts of stress. Healing of tendons is divided into three overlapping phases: inflammation, proliferation/repair, and remodeling. Treatment remains challenging, with current strategies ranging from conservative approaches such as eccentric loading and physical therapy to regenerative interventions like platelet-rich plasma (PRP), stem cell therapies, injectable corticosteroids, and low-energy laser therapy. Understanding the underlying biological mechanisms of tendon degeneration is critical for developing targeted therapies and improving long-term outcomes.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"17"},"PeriodicalIF":3.1,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prox1 maintains taste bud structure via inhibition of apoptosis.","authors":"Aya Hagimoto, Eriko Koyanagi-Matsumura, Norihito Oura, Mitsuru Saito, Tatsurou Tanaka, Hirohito Miura","doi":"10.1007/s00441-025-04040-7","DOIUrl":"10.1007/s00441-025-04040-7","url":null,"abstract":"<p><p>Taste buds are maintained by continuous cell renewal, receiving a steady influx of postmitotic cells from the surrounding epithelial region. Within taste buds, Type I, II, and III cells continuously differentiate from Type IV postmitotic precursor cells and are removed via apoptosis. These processes are likely governed by various transcription factors. Among the transcription factors expressed in taste buds, Prox1, a homeobox transcription factor, is the only factor expressed in all taste bud cells including precursor cells. However, its role in taste buds remains unclear. Here, we investigate the function of Prox1 in taste bud cell turnover using conditional knockout (cKO) mice. In Prox1 cKO mice, all Type I, II, and III cells were significantly reduced, resulting in approximately half the total cell number per taste bud compared to wild-type mice, while Type IV cell numbers remained comparable. Apoptosis of taste bud cells nearly doubled, leading to a shortened lifespan of taste bud cells. EdU pulse-labeling experiments revealed a biphasic decline in EdU(+) taste bud cells in Prox1 cKO mice, indicating that Prox1 knockout increases the fraction of cells that die shortly after differentiating into taste bud cells. The surviving cells still exhibited a shorter lifespan than that of wild-type mice. We also observed previously unreported structural alterations within taste buds caused by enhanced apoptosis using whole-mount analysis. These results demonstrate that Prox1 contributes to the maintenance of taste bud structure by regulating the lifespan of taste bud cells, highlighting its essential role in taste bud homeostasis.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"16"},"PeriodicalIF":3.1,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12872690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in secondary lipids are associated with neuroinflammation in the brain of Neu1-deficient mice.","authors":"Ebru Ada, Volkan Seyrantepe","doi":"10.1007/s00441-026-04045-w","DOIUrl":"10.1007/s00441-026-04045-w","url":null,"abstract":"<p><p>Neu1 (lysosomal sialidase 1) is essential for removing sialic acid from oligosaccharides and glycoconjugates. Neu1 deficiency impairs lysosomal digestion, leading to sialidosis and sialoglycoprotein accumulation. It also increases lipids, including gangliosides GM3, GD3, GM4, and LM1, in the kidney, liver, and spleen. Neu1-/- mice display symptoms resembling Type II sialidosis, including enlarged spleen and liver, kidney issues, neurological problems, spinal defects, and oligosaccharide buildup. The study examined secondary lipid alterations and inflammation in the cortex and cerebellum of these mice. Lipidomic, molecular, and immunohistochemical analyses of tissues from 2 and 5 M Neu1-/- mice revealed reduced levels of lipids, including PC, PE, PS, and CL, along with increased pro-inflammatory cytokines and loss of oligodendrocytes and neurons. Signs of astrogliosis and microgliosis emerged in specific brain regions. These results indicate that reduced levels of glycerophospholipids could serve as an indicator of inflammation in sialidosis mice. Future research should investigate therapies targeting these lipid changes, as modulating glycerophospholipids might slow disease progression in sialidosis patients.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"15"},"PeriodicalIF":3.1,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rab24 protein levels show dynamic changes in mouse tissues and human cancers.","authors":"H G Mauricio Ramm, Farhad Ahmed, Sadaf Fazeli, Matthieu Bourgery, Martin Alexander Lopez, Lav Tripathi, Ilmo Leivo, Pernilla Syrjä, Eeva-Liisa Eskelinen","doi":"10.1007/s00441-025-04043-4","DOIUrl":"10.1007/s00441-025-04043-4","url":null,"abstract":"<p><p>Rab24 is an unusual member of the Rab family of small GTPases, implicated in autophagy, endocytosis and cell division. In order to elucidate possible organ and age-specific roles of Rab24, we investigated tissue-specific levels of Rab24 in mice by western blotting and immunohistochemistry from postnatal day one to 9 months of age. In adult mice, the highest protein levels were found in the brain followed by the kidney, whereas lower levels were detected in the pancreas, spleen, liver, lung, heart, and skeletal muscle. Dynamic changes in Rab24 levels were observed during early postnatal development, with a sharp increase in the brain at postnatal day 14, after which the level remained high into adulthood. In the heart, skeletal muscle, pancreas and liver, higher Rab24 levels were observed during the first two postnatal weeks, after which the levels dropped and stayed low until adulthood. The age-dependent changes suggest age- and organ-specific regulation of Rab24 protein levels and possible organ-specific roles for Rab24 in development and maturation. Immunohistochemistry of the brain revealed that Rab24 was mostly present in neuronal cells in 1-month-old and older mice. Also, epithelial cells in several tissues showed high Rab24 levels. These results suggest possible roles for Rab24 in neuronal and epithelial maintenance. We further analysed immunohistochemical staining for RAB24 in human cancers and normal tissues. RAB24 staining in cancers of the breast and skin was higher than in the corresponding normal tissues, while it was reduced in cancers of the digestive system and the urinary tract. We also observed elevated RAB24 staining in medulloblastoma and neuroblastoma, two paediatric cancers of neuronal origin. In pancreatic neuroendocrine tumours that originate from islet cells, RAB24 levels were lower than in normal pancreatic islet cells. Collectively, our findings provide a comprehensive overview of RAB24 protein levels across mouse tissues and a wide spectrum of human cancers. The observed differences in RAB24 levels between cancer types and between malignant and normal tissues, suggest that RAB24 may serve as a potential diagnostic or differentiation marker in specific tumour types.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"14"},"PeriodicalIF":3.1,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12847187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146050557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pannexin-1 is present in a subpopulation of bovine milk-derived small extracellular vesicles.","authors":"Md Ruhul Amin, Spencer R Marsh, Claire Beard, Laura Beth Payne, Amanda Charest, Randy F Stout, Robert G Gourdie","doi":"10.1007/s00441-026-04044-x","DOIUrl":"10.1007/s00441-026-04044-x","url":null,"abstract":"<p><p>Pannexin-1 (Panx1) is a large-pore membrane channel protein implicated in diverse physiological and pathological processes, yet its presence in extracellular vesicles has not been fully characterized. In this study, we investigated small extracellular vesicles derived from bovine milk to determine whether they contain Panx1. Using multiple complementary approaches, we show that Panx1 is present in a distinct subpopulation of milk-derived vesicles. Panx1 immunoreactivity was detected by Western blotting with antibodies targeting both the C-terminal and extracellular loop regions, and single-molecule localization microscopy revealed that a subpopulation of CD9-positive mEVs expresses Panx1. Consistently, flow cytometry-based analysis indicated that approximately half of the vesicle population was Panx1 positive. Together, these findings provide the first validated evidence that Panx1 is incorporated into bovine milk-derived small extracellular vesicles, expanding the repertoire of membrane channel proteins associated with extracellular vesicles and establishing a foundation for future studies on the functional role of Panx1 in vesicle biology and vesicle-mediated signaling.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 2","pages":"13"},"PeriodicalIF":3.1,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12847120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146050504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenothiazine alleviates cisplatin-mediated acute kidney injury by inhibiting ferroptosis.","authors":"Xue Sun, Yang Chen, Chong Wei, Junyi Shao, Jingshu Min, Feiyang Zhao, Chaoyi Xia","doi":"10.1007/s00441-025-04037-2","DOIUrl":"10.1007/s00441-025-04037-2","url":null,"abstract":"<p><p>Cisplatin is a first-line chemotherapeutic agent for various tumors; however, its clinical utility is substantially limited by the risk of cisplatin-induced acute kidney injury (AKI). Since inhibition of ferroptosis effectively mitigates cisplatin toxicity, we aimed to expand the therapeutic window of cisplatin by identifying potent ferroptosis inhibitors. High-throughput quantitative cell imaging-based screening of an FDA-approved drug library identified phenothiazine (PTZ) as a promising ferroptosis inhibitor. We further validated the ferroptosis-inhibitory activity of PTZ under both GPX4 inhibition and cystine deprivation conditions. Notably, PTZ administration markedly attenuated cisplatin-induced AKI and dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD) in mouse models. These findings indicate that PTZ holds clinical potential for reducing cisplatin-associated nephrotoxicity, thereby broadening the therapeutic applicability of cisplatin, as well as for treating IBD. Given its robust anti-ferroptosis effects, PTZ may also provide therapeutic benefits in other ferroptosis-related pathologies. Collectively, this study identifies PTZ as a promising lead compound for the development of ferroptosis-targeted therapeutics.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"403 1","pages":"12"},"PeriodicalIF":3.1,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146028496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}