Cell and Tissue Research最新文献_第6页

Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges. 钙质海绵中完整组织和再生过程中的微管组织和微管蛋白翻译后修饰。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-05 DOI: 10.1007/s00441-025-03960-8

Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov

{"title":"Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.","authors":"Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov","doi":"10.1007/s00441-025-03960-8","DOIUrl":"10.1007/s00441-025-03960-8","url":null,"abstract":"Microtubules are the principal cytoskeletal component in cells, integral to various morphogenetic processes in Metazoa, including cell migration, adhesion, and polarity. Their dynamics and functions are modulated by tubulin post-translational modifications (PTMs). While studies on model species have provided insights into microtubule functions, understanding their evolutionary aspects necessitates exploring non-model organisms. Sponges (phylum Porifera) are an early-branching metazoan group with outstanding regenerative capacities. This research presents the first comprehensive analysis of microtubule organization and tubulin PTMs in calcareous sponges. The intact sponge cells show various but typical types of microtubule organization, while detected tubulin PTMs are associated with certain cell types, indicating specific functions in particular cellular contexts. During regeneration, relying on the coordinated movement of epithelial-like cell sheets, microtubule networks in exopinacocytes and choanocytes undergo significant reorganization. These rearranged microtubules potentially stabilize cellular migration direction and facilitate cargo transport, essential for cell contact and polarity establishment. This study enhances our understanding of microtubule functionality and regulation in early-diverging metazoans, contributing to the broader evolutionary context of cytoskeletal dynamics.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"365-381"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of hydroxyapatite nanoparticles on the cellular processes of stem cells derived from dental tissue sources. 羟基磷灰石纳米颗粒对牙组织来源干细胞细胞过程的影响。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-18 DOI: 10.1007/s00441-025-03962-6

Mais Emad, Mohammad Alnatour, Walhan Alshaer, Jennifer L Gibbs, Benoît Michot, Dana Alqudah, Alaa A A Aljabali, Mairvat Al-Mrahleh, Abdolelah Jaradat, Duaa Abuarqoub

{"title":"Impact of hydroxyapatite nanoparticles on the cellular processes of stem cells derived from dental tissue sources.","authors":"Mais Emad, Mohammad Alnatour, Walhan Alshaer, Jennifer L Gibbs, Benoît Michot, Dana Alqudah, Alaa A A Aljabali, Mairvat Al-Mrahleh, Abdolelah Jaradat, Duaa Abuarqoub","doi":"10.1007/s00441-025-03962-6","DOIUrl":"10.1007/s00441-025-03962-6","url":null,"abstract":"Hydroxyapatite nanoparticle (HANPs) utilization has recently been notable in bone tissue engineering. This surge owes itself to the biocompatibility of HANPs and their striking resemblance to the minerals found in natural bone. Furthermore, dental pulp-derived stem cells (DPSCs) have garnered attention due to their remarkable differentiation potential into multilineages, thus positioning them as a pivotal cell reservoir for regenerative medicine. This study aims to investigate the impact of HANPs on DPSCs cellular processes. The HANPs have been synthesized using the wet chemical precipitation method followed by freeze-drying and characterization using dynamic light scattering (DLS) and transmission electron microscopy (TEM). The size of HANPs was reported to be in the range of 55-67 nm. Our dataset divulges that DPSCs can endure concentrations of HANPs up to ≤ 0.81 mg/mL without incurring any conspicuous alterations in their morphology or the pace of proliferation. Furthermore, the self-renewal potency of HANPs was upheld at concentrations ≤ 0.20 mg/mL. Flow cytometric analysis affirms a significant divergence in cell distribution across all cell cycle phases in DPSCs treated with 0.81 mg/mL HANPs. Intriguingly, no variance surfaced in the migratory capacity of DPSCs exposed to HANPs of ≤ 0.40 mg/mL. For osteogenic differentiation, HANPs at concentrations of ≤ 0.40 mg/mL demonstrated the aptitude to incite osteogenic differentiation within DPSCs, facilitating the formation of calcium deposits. In conclusion, combining HANPs and DPSCs shows promise for restoring damaged hard tissues, like bone and teeth, and enhancing regenerative therapies.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"319-330"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered morphology of mucosa-associated lymphoid tissues and epithelium in the nasal cavity and lacrimal apparatus in autoimmune disease-prone MRL/MpJ-Fas^lpr/lpr mice. 自身免疫性疾病易发MRL/MpJ-Faslpr/lpr小鼠鼻腔和泪器粘膜相关淋巴组织和上皮形态的改变

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-25 DOI: 10.1007/s00441-025-03966-2

Masaya Hiraishi, Takashi Namba, Teppei Nakamura, Md Zahir Uddin Rubel, Yasuhiro Kon, Osamu Ichii

{"title":"Altered morphology of mucosa-associated lymphoid tissues and epithelium in the nasal cavity and lacrimal apparatus in autoimmune disease-prone MRL/MpJ-Faslpr/lpr mice.","authors":"Masaya Hiraishi, Takashi Namba, Teppei Nakamura, Md Zahir Uddin Rubel, Yasuhiro Kon, Osamu Ichii","doi":"10.1007/s00441-025-03966-2","DOIUrl":"10.1007/s00441-025-03966-2","url":null,"abstract":"The mucosal epithelium and the mucosa-associated lymphoid tissues (MALTs) protect the mucosa, such as eye and nose, from constant exposure to foreign antigens. As autoimmune disorders can target both epithelium and MALTs, we morphologically investigated the head of MRL/MpJ-Faslpr/lpr, an autoimmune disease-prone mouse, to discuss the pathological crosstalk among autoimmune disorders, mucosal epithelium, and MALTs. Compared to healthy control MRL/MpJ mice, MRL/MpJ-Faslpr/lpr mice had more lymphoid tissues that were diffusely localized beneath the mucosa epithelium in their lacrimal tracts and nasal cavity. Particularly, lacrimal duct- and nasopharynx-associated lymphoid tissues (LDALT, NALT) were identified as the major MALTs in the mouse head. In the nasal mucosa, MRL/MpJ-Faslpr/lpr mice exhibited larger NALT, more frequent non-ciliated epithelial cells, and more goblet cells than in MRL/MpJ mice. NALT in MRL/MpJ-Faslpr/lpr mice contained more proliferating cells than in MRL/MpJ mice. Moreover, LDALT in some MRL/MpJ-Faslpr/lpr mice developed by being directly connected to the bone marrow surrounding the nasolacrimal duct. These data suggested systemic autoimmune disorders could alter the mucosal immunity of the head by affecting both mucosal epithelium and MALTs. These findings are crucial for understanding the pathology of the head mucosal immunity.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"331-345"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity-driven musculotendinous remodeling impairs tissue resilience to mechanical damage. 肥胖驱动的肌肉肌腱重塑损害组织对机械损伤的恢复力。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-31 DOI: 10.1007/s00441-025-03967-1

Cesanelli L, Minderis P, Balnyte I, Ratkevicius A, Degens H, Satkunskiene D

{"title":"Obesity-driven musculotendinous remodeling impairs tissue resilience to mechanical damage.","authors":"Cesanelli L, Minderis P, Balnyte I, Ratkevicius A, Degens H, Satkunskiene D","doi":"10.1007/s00441-025-03967-1","DOIUrl":"10.1007/s00441-025-03967-1","url":null,"abstract":"Obesity has been associated with lower muscle strength-to-body mass ratio. Here, we evaluated the effects of diet-induced obesity on the mechano-structural properties of isolated muscles and tendons. Thirty 10-week-old male C57BL/6 J mice were randomly assigned to either an obesogenic high-fat diet group (OB) for 24 weeks or a control group (CN) maintained on a standard chow diet. Soleus muscle (SOL) and Achilles tendon (AT) specimens were isolated and subjected either to failure testing, 300 cycles of passive stretch-destretch, or isometric twitch contractions. Morpho-structural and protein expression analyses were conducted to assess collagen and adipose tissue accumulation, concentrations of cross-linking factors, and any alterations in the POSTN-TGFβ1-Akt signaling pathway. OB SOL and AT tissues were more fragile than those from CN (p < 0.05). A piecewise linear regression model revealed a tendency for OB tissues to exhibit steeper mechanical property changes within the first 20 cycles compared to CN, followed by a similar plateau phase in both groups. OB SOL-AT complexes showed a slower twitch-contraction-relaxation pattern than CN (p < 0.05). OB tendons and muscles were larger than those of the CN, with muscles featuring bigger fibers, and higher collagen area fraction (p < 0.05). Elevated TGFβ1 and POSTN concentrations were observed in OB tissues (p < 0.05), alongside increased P-Akt and P-4EBP1 expression (p < 0.05). These findings highlight the detrimental effects of obesity on the structural integrity of muscle and tendon tissues and suggest a significant role of POSTN-TGFβ1-Akt signaling in obesity-associated musculotendinous remodeling.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"287-302"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attenuation of intestinal peristalsis with age is attributed to decreased sensitivity of receptors in the enteric nervous system. 随着年龄的增长，肠蠕动的衰减是由于肠神经系统中受体的敏感性降低。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 Epub Date: 2025-03-27 DOI: 10.1007/s00441-025-03969-z

Tsukasa Kobayashi, Yuko Takeba, Masanori Ootaki, Yuki Ohta, Keisuke Kida, Taroh Iiri, Naoki Matsumoto

{"title":"Attenuation of intestinal peristalsis with age is attributed to decreased sensitivity of receptors in the enteric nervous system.","authors":"Tsukasa Kobayashi, Yuko Takeba, Masanori Ootaki, Yuki Ohta, Keisuke Kida, Taroh Iiri, Naoki Matsumoto","doi":"10.1007/s00441-025-03969-z","DOIUrl":"10.1007/s00441-025-03969-z","url":null,"abstract":"Constipation and other digestive disorders are common in older adults. The autonomic nervous system plays a critical role in regulating digestive motility in the intestinal tract. However, studies on age-related changes in autonomic function and receptor expression in the intestinal tract are limited. In this study, we examined the expression of neurotransmitter receptors in the autonomic nervous system and the effects of acetylcholine and β-agonists on intestinal contraction and relaxation in the jejunum of aged rats. Jejunal sections collected from male and female Wistar/ST rats aged 4, 11, and 18 months were analyzed. Immunohistochemical staining and enzyme-linked immunosorbent assay were used to measure the expression of muscarinic acetylcholine receptors (CHRM2 and CHRM3) and β-adrenergic receptors (β2-ADR and β3-ADR). The effects of acetylcholine, isoproterenol, and mirabegron were assessed in the isolated jejunum for each age group. There was no significant difference in CHRM2 receptor expression among the age groups; however, CHRM3 receptor expression decreased with age. Additionally, the sensitivity to acetylcholine-induced contractile responses decreased with age. Although β2-ADR receptor expression did not differ among the age groups, β3-ADR receptor expression increased with age. Despite this, the relaxation response to isoproterenol and mirabegron decreased with age. Our study revealed an age-related decrease in CHRM3 expression and the contractile response to acetylcholine in the small intestine of rats. Although β-ADR expression, particularly β3-ADR, increased with age, the relaxation response to β-adrenergic agonists gradually decreased.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"303-317"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Microtubule organization and tubulin post‑translational modifications in intact tissues and during regeneration in calcareous sponges. 对完整组织和钙质海绵再生过程中微管组织和微管蛋白翻译后修饰的修正。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 DOI: 10.1007/s00441-025-03964-4

Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov

引用次数: 0

Correction to: Long noncoding RNA XIST inhibition promotes Leydig cell apoptosis by acting as a competing endogenous RNA for microRNA‑145a‑5p that targets SIRT1 in late‑onset hypogonadism. 更正：长链非编码RNA XIST抑制通过作为microRNA - 145a - 5p的竞争内源性RNA促进间质细胞凋亡，microRNA - 145a - 5p在晚发性性腺功能减退中靶向SIRT1。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-06-01 DOI: 10.1007/s00441-025-03963-5

Jing Wang, Yiqiong Yang, Yang Xu, Zhipeng Xu, Xiaozhi Zhao, Ruipeng Jia, Yutian Dai

引用次数: 0

A historical perspective of Kupffer cells in the context of infection. 从历史角度看感染背景下的 Kupffer 细胞。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-01 Epub Date: 2024-10-11 DOI: 10.1007/s00441-024-03924-4

Carolyn T Graham, Siamon Gordon, Paul Kubes

引用次数: 0

Roots and early routes of neuroendocrinology. 神经内分泌学的根源和早期途径。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-01 Epub Date: 2025-01-30 DOI: 10.1007/s00441-024-03928-0

Esteban M Rodríguez, Montserrat Guerra, Juan Luis Blázquez

{"title":"Roots and early routes of neuroendocrinology.","authors":"Esteban M Rodríguez, Montserrat Guerra, Juan Luis Blázquez","doi":"10.1007/s00441-024-03928-0","DOIUrl":"10.1007/s00441-024-03928-0","url":null,"abstract":"Carl C. Speidel (1919) and Ernst Scharrer (1928) were privileged witnesses of the encounter between neurons and hormones, a biological phenomenon that had been occurring in nature during millions of years of evolution, as Berta Scharrer started to unfold since 1935 on. The story of neurosecretion is intimately associated to that of the hypothalamus, such a \"marvellous region\", as Wolfgang Bargmann (1975) called it. This story started more than two millennia ago. We have made an effort to trace the roots of the discoveries that gave rise to a medical discipline, neuroendocrinology. Our trip to the roots covers a period from the fourth century BC, when an extraordinary Medical School was founded in Alexandria, and extends into the late 1970s of the twentieth century, when neuroendocrine research had started to grow exponentially. An effort has been made to track back the origin of each piece of knowledge that was constructing, brick upon brick, the building of this new medical science, hoping that it would help neuroendocrinologists of the new era to find their own roots, to meet their ancestors. Tracking the roots of a particular phenomenon provides the opportunity to have an overview of the whole phenomenon, allowing comprehension rather than merely knowledge. An important purpose pursued throughout this article was to pay a tribute to all those who, in the early days, contributed to the brain-endocrine encounter. We have tried our best to bring back the achievements of most of them.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"137-215"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

100 years Cell and Tissue Research: the founders and their successors. 100年细胞和组织研究：创始人和他们的继任者。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-01 Epub Date: 2025-01-27 DOI: 10.1007/s00441-025-03950-w

Klaus Unsicker

引用次数: 0