Cell and Tissue Research最新文献_第6页

Entry of cannabidiol into the fetal, postnatal and adult rat brain. 大麻二酚进入胎儿、出生后和成年大鼠大脑的情况。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-02-17 DOI: 10.1007/s00441-024-03867-w

Georgia Fitzpatrick, Yifan Huang, Fiona Qiu, Mark D Habgood, Robert L Medcalf, Heidi Ho, Katarzyna M Dziegielewska, Norman R Saunders

{"title":"Entry of cannabidiol into the fetal, postnatal and adult rat brain.","authors":"Georgia Fitzpatrick, Yifan Huang, Fiona Qiu, Mark D Habgood, Robert L Medcalf, Heidi Ho, Katarzyna M Dziegielewska, Norman R Saunders","doi":"10.1007/s00441-024-03867-w","DOIUrl":"10.1007/s00441-024-03867-w","url":null,"abstract":"Cannabidiol is a major component of cannabis but without known psychoactive properties. A wide range of properties have been attributed to it, such as anti-inflammatory, analgesic, anti-cancer, anti-seizure and anxiolytic. However, being a fairly new compound in its purified form, little is known about cannabidiol brain entry, especially during development. Sprague Dawley rats at four developmental ages: embryonic day E19, postnatal day P4 and P12 and non-pregnant adult females were administered intraperitoneal cannabidiol at 10 mg/kg with [3H] labelled cannabidiol. To investigate the extent of placental transfer, the drug was injected intravenously into E19 pregnant dams. Levels of [3H]-cannabidiol in blood plasma, cerebrospinal fluid and brain were estimated by liquid scintillation counting. Plasma protein binding of cannabidiol was identified by polyacrylamide gel electrophoresis and its bound and unbound fractions measured by ultrafiltration. Using available RNA-sequencing datasets of E19 rat brain, choroid plexus and placenta, as well as P5 and adult brain and choroid plexus, expression of 13 main cannabidiol receptors was analysed. Results showed that cannabidiol rapidly entered both the developing and adult brains. Entry into CSF was more limited. Its transfer across the placenta was substantially restricted as only about 50% of maternal blood plasma cannabidiol concentration was detected in fetal plasma. Albumin was the main, but not exclusive, cannabidiol binding protein at all ages. Several transcripts for cannabidiol receptors were expressed in age- and tissue-specific manner indicating that cannabidiol may have different functional effects in the fetal compared to adult brain.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"177-195"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11055756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139746240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracrine and endocrine pathways of natriuretic peptides assessed by ligand-receptor mapping in the Japanese eel brain. 通过配体-受体映射评估日本鳗鱼大脑中钠利尿肽的旁分泌和内分泌途径。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-02-19 DOI: 10.1007/s00441-024-03873-y

Tomoki Izumi, Ami Saito, Takanori Ida, Takao Mukuda, Yukitoshi Katayama, Marty Kwok-Shing Wong, Takehiro Tsukada

{"title":"Paracrine and endocrine pathways of natriuretic peptides assessed by ligand-receptor mapping in the Japanese eel brain.","authors":"Tomoki Izumi, Ami Saito, Takanori Ida, Takao Mukuda, Yukitoshi Katayama, Marty Kwok-Shing Wong, Takehiro Tsukada","doi":"10.1007/s00441-024-03873-y","DOIUrl":"10.1007/s00441-024-03873-y","url":null,"abstract":"The natriuretic peptide (NP) family consists of cardiac NPs (ANP, BNP, and VNP) and brain NPs (CNPs) in teleosts. In addition to CNP1-4, a paralogue of CNP4 (named CNP4b) was recently discovered in basal teleosts including Japanese eel. Mammals have lost most Cnps during the evolution, but teleost cnps were conserved and diversified, suggesting that CNPs are important hormones for maintaining brain functions in teleost. The present study evaluated the potency of each Japanese eel CNP to their NP receptors (NPR-A, NPR-B, NPR-C, and NPR-D) overexpressed in CHO cells. A comprehensive brain map of cnps- and nprs-expressing neurons in Japanese eel was constructed by integrating the localization results obtained by in situ hybridization. The result showed that CHO cells expressing NPR-A and NPR-B induced strong cGMP productions after stimulation by cardiac and brain NPs, respectively. Regarding brain distribution of cnps, cnp1 is engaged in the ventral telencephalic area and periventricular area including the parvocellular preoptic nucleus (Pp), anterior/posterior tuberal nuclei, and periventricular gray zone of the optic tectum. cnp3 is found in the habenular nucleus and prolactin cells in the pituitary. cnp4 is expressed in the ventral telencephalic area, while cnp4b is expressed in the motoneurons in the medullary area. Such CNP isoform-specific localizations suggest that function of each CNP has diverged in the eel brain. Furthermore, the Pp lacking the blood-brain barrier expressed both npra and nprb, suggesting that endocrine and paracrine NPs interplay for regulating the Pp functions in Japanese eels.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"197-212"},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KDELR2 promotes bone marrow mesenchymal stem cell osteogenic differentiation via GSK3β/β-catenin signaling pathway. KDELR2通过GSK3β/β-catenin信号通路促进骨髓间充质干细胞成骨分化。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-03-12 DOI: 10.1007/s00441-024-03884-9

Xiaoyong Wu, Weijun Zhang, Long Long, Yibo Wang, Hongyu Chen, Kanbin Wang, Zhongxiang Wang, Jinwu Bai, Deting Xue, Zhijun Pan

引用次数: 0

Correction to: Nescient helix-loop-helix 1 (Nhlh1) is a novel activating transcription factor 5 (ATF5) target gene in olfactory and vomeronasal sensory neurons in mice. 更正：Nescient helix-loop-helix 1 (Nhlh1) 是小鼠嗅觉和绒毛感觉神经元中一种新型激活转录因子 5 (ATF5) 的靶基因。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 DOI: 10.1007/s00441-024-03891-w

Chiharu Ishii, Haruo Nakano, Riko Higashiseto, Yusaku Ooki, Mariko Umemura, Shigeru Takahashi, Yuji Takahashi

引用次数: 0

Gadolinium retention effect on macrophages — a potential cause of MRI contrast agent Dotarem toxicity 钆对巨噬细胞的滞留效应--磁共振成像造影剂多他雷毒性的潜在原因

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-16 DOI: 10.1007/s00441-024-03885-8

Marta Halasa, Ahmed Uosef, Henry V. Ubelaker, Arijita Subuddhi, Krupa R. Mysore, Jacek Z. Kubiak, Rafik M. Ghobrial, Jarek Wosik, Malgorzata Kloc

{"title":"Gadolinium retention effect on macrophages — a potential cause of MRI contrast agent Dotarem toxicity","authors":"Marta Halasa, Ahmed Uosef, Henry V. Ubelaker, Arijita Subuddhi, Krupa R. Mysore, Jacek Z. Kubiak, Rafik M. Ghobrial, Jarek Wosik, Malgorzata Kloc","doi":"10.1007/s00441-024-03885-8","DOIUrl":"https://doi.org/10.1007/s00441-024-03885-8","url":null,"abstract":"Gadolinium is a component of the MRI contrast agent Dotarem. Although Dotarem is the least toxic among MRI contrasts used, gadolinium present in Dotarem accumulates for many years in various organs and tissues exerting toxic effects. We showed previously that gadolinium remains in macrophages for at least 7 days after exposure to Dotarem. However, very little is known about the effect of gadolinium retention on the immune cells such as macrophages. We studied the effect of 1-day and 7-day retention of gadolinium on various functions and molecular pathways of macrophages. Gadolinium retention for 7 days decreased macrophage adhesion and motility and dysregulated the expression of adhesion and fibrotic pathway-related proteins such as Notch1 and its ligand Jagged1, adhesion/migration-related proteins PAK1 and Shp1, immune response-related transcription factors Smad3 and TCF19, and chemokines CXCL10 and CXCL13, and dysregulated the mRNA expression of fibrosis-related genes involved in extracellular matrix (ECM) synthesis, such as Col6a1, Fibronectin, MMP9, and MMP12. It also completely (below a level of detection) shut down the transcription of anti-inflammatory M2 macrophage polarization marker the Arg-1. Such changes, if they occur in MRI patients, can be potentially detrimental to the patient’s immune system and immune response-related processes.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"40 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140615275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD34+ synovial fibroblasts exhibit high osteogenic potential in synovial chondromatosis CD34+ 滑膜成纤维细胞在滑膜软骨瘤病中表现出很高的成骨潜能

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-11 DOI: 10.1007/s00441-024-03892-9

Xiaoyu Li, Hao Sun, Deng Li, Zhiqing Cai, Jie Xu, Ruofan Ma

{"title":"CD34+ synovial fibroblasts exhibit high osteogenic potential in synovial chondromatosis","authors":"Xiaoyu Li, Hao Sun, Deng Li, Zhiqing Cai, Jie Xu, Ruofan Ma","doi":"10.1007/s00441-024-03892-9","DOIUrl":"https://doi.org/10.1007/s00441-024-03892-9","url":null,"abstract":"Synovial chondromatosis (SC) is a disorder of the synovium characterized by the formation of osteochondral nodules within the synovium. This study aimed to identify the abnormally differentiated progenitor cells and possible pathogenic signaling pathways. Loose bodies and synovium were obtained from patients with SC during knee arthroplasty. Single-cell RNA sequencing was used to identify cell subsets and their gene signatures in SC synovium. Cells derived from osteoarthritis (OA) synovium were used as controls. Multi-differentiation and colony-forming assays were used to identify progenitor cells. The roles of transcription factors and signaling pathways were investigated through computational analysis and experimental verification. We identified an increased proportion of CD34+ sublining fibroblasts in SC synovium. CD34+CD31− cells and CD34−CD31− cells were sorted from SC synovium. Compared with CD34− cells, CD34+ cells had larger alkaline phosphatase (ALP)-stained area and calcified area after osteogenic induction. In addition, CD34+ cells exhibited a stronger tube formation ability than CD34− cells. Our bioinformatic analysis suggested the expression of TWIST1, a negative regulator of osteogenesis, in CD34− sublining fibroblasts and was regulated by the TGF-β signaling pathway. The experiment showed that CD34+ cells acquired the TWIST1 expression during culture and the combination of TGF-β1 and harmine, an inhibitor of Twist1, could further stimulate the osteogenesis of CD34+ cells. Overall, CD34+ synovial fibroblasts in SC synovium have multiple differentiation potentials, especially osteogenic differentiation potential, and might be responsible for the pathogenesis of SC.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"2 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140568067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression of neuropeptide F in the digestive organs of female freshwater prawn, Macrobrachium rosenbergii, during the ovarian cycle 雌性淡水对虾消化器官中神经肽 F 在卵巢周期中的差异表达

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-09 DOI: 10.1007/s00441-024-03893-8

Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul

{"title":"Differential expression of neuropeptide F in the digestive organs of female freshwater prawn, Macrobrachium rosenbergii, during the ovarian cycle","authors":"Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul","doi":"10.1007/s00441-024-03893-8","DOIUrl":"https://doi.org/10.1007/s00441-024-03893-8","url":null,"abstract":"Neuropeptide F is a key hormone that controls feeding in invertebrates, including decapod crustaceans. We investigated the differential expression of Macrobrachium rosenbergii neuropeptide F (MrNPF) in the digestive organs of female prawns, M. rosenbergii, during the ovarian cycle. By using RT-qPCR, the expression of MrNPF mRNA in the esophagus (ESO), cardia (CD), and pylorus (PY) of the foregut (FG) gradually increased from stage II and peaked at stage III. In the midgut (MG), hindgut (HG), and hepatopancreas (HP), MrNPF mRNA increased from stage I, reaching a maximal level at stage II, and declined by about half at stages III and IV (P < 0.05). In the ESO, CD, and PY, strong MrNPF-immunoreactivities were seen in the epithelium, muscle, and lamina propria. Intense MrNPF-ir was found in the MG cells and the muscular layer. In the HG, MrNPF-ir was detected in the epithelium of the villi and gland regions, while MrNPF-ir was also more intense in the F-, R-, and B-cells in the HP. However, we found little colocalization between the MrNPF and PGP9.5/ChAT in digestive tissues, implying that most of the positive cells might not be neurons but could be digestive tract-associated endocrine cells that produce and secrete MrNPF to control digestive organ functions in feeding and utilizing feed. Taken together, our first findings indicated that MrNPF was differentially expressed in digestive organs in correlation with the ovarian cycle, suggesting an important link between MrNPF, the physiology of various digestive organs in feeding, and possibly ovarian maturation in female M. rosenbergii.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"103 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140568157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of ANO1 currents in pacemaking of PDGFRα-positive specialised smooth muscle cells in rat caudal epididymis ANO1 电流参与了大鼠尾侧附睾 PDGFRα 阳性特化平滑肌细胞的起搏过程

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-08 DOI: 10.1007/s00441-024-03890-x

Wataru Kudo, Retsu Mitsui, Hikaru Hashitani

{"title":"Involvement of ANO1 currents in pacemaking of PDGFRα-positive specialised smooth muscle cells in rat caudal epididymis","authors":"Wataru Kudo, Retsu Mitsui, Hikaru Hashitani","doi":"10.1007/s00441-024-03890-x","DOIUrl":"https://doi.org/10.1007/s00441-024-03890-x","url":null,"abstract":"The epididymal duct exhibits spontaneous phasic contractions (SPCs) to store and transport sperm. Here, we explored molecular identification of pacemaker cells driving SPCs in the caudal epididymal duct and also investigated properties of pacemaker currents underlying SPCs focusing on ANO1 Ca2+-activated Cl− channels (CaCCs). Immunohistochemistry was performed to visualise the distribution of platelet-derived growth factor receptor α (PDGFRα)- or ANO1-positive cells in the rat caudal epididymal duct. Perforated whole-cell patch clamp technique was applied to enzymatically isolated epididymal cells, while SPCs were recorded with video edge-tracking technique. Immunohistochemistry revealed the distribution of α-smooth muscle actin (α-SMA)-positive cells co-expressing both PDGFRα and ANO1 in the innermost smooth muscle layer. Approximately one-third of isolated epididymis cells exhibited spontaneous transient inward currents (STICs) at the holding potential −60 mV. The reversal potential for STICs was close to the calculated chloride equivalent potential depending on intracellular Cl− concentrations. Ani9 (3 µM), the ANO1 specific inhibitor, decreased both amplitude and frequency of STICs, while cyclopiazonic acid (CPA, 30 µM), a sarco-/endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitor, abolished STICs. Ani9 (3 or 10 µM) reduced the frequency of SPCs without changing their amplitude. Thus, PDGFRα+, ANO1+ specialised smooth muscle cells (SMCs) appear to function as pacemaker cells to electrically drive epididymal SPCs by generating ANO1-dependnet STICs. STICs arising from spontaneous Ca2+ release from intracellular Ca2+ store and subsequent opening of ANO1 result in depolarisations that spread into adjacent SMCs where L-type voltage-dependent Ca2+ channels are activated to develop SPCs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"2 1","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140568159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Induction of forkhead box M1 (FoxM1) by EGF through ERK signaling pathway promotes trophoblast cell invasion 更正为EGF通过ERK信号通路诱导叉头盒M1（FoxM1）促进滋养层细胞侵袭

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-05 DOI: 10.1007/s00441-024-03889-4

Yunpeng Xie, Dan Cui, Linlin Sui, Yuefei Xu, Ningning Zhang, Yanni Ma, Yinghua Li, Ying Kong

引用次数: 0

Dynamic changes in endoplasmic reticulum morphology and its contact with the plasma membrane in motor neurons in response to nerve injury. 运动神经元内质网形态及其与质膜接触的动态变化对神经损伤的反应

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-01 Epub Date: 2024-02-05 DOI: 10.1007/s00441-024-03858-x

Mahmoud Elgendy, Hiromi Tamada, Takaya Taira, Yuma Iio, Akinobu Kawamura, Ayusa Kunogi, Yuka Mizutani, Hiroshi Kiyama

{"title":"Dynamic changes in endoplasmic reticulum morphology and its contact with the plasma membrane in motor neurons in response to nerve injury.","authors":"Mahmoud Elgendy, Hiromi Tamada, Takaya Taira, Yuma Iio, Akinobu Kawamura, Ayusa Kunogi, Yuka Mizutani, Hiroshi Kiyama","doi":"10.1007/s00441-024-03858-x","DOIUrl":"10.1007/s00441-024-03858-x","url":null,"abstract":"The endoplasmic reticulum (ER) extends throughout a cell and plays a critical role in maintaining cellular homeostasis. Changes in ER shape could provide a clue to explore the mechanisms that underlie the fate determination of neurons after axon injury because the ER drastically changes its morphology under neuronal stress to maintain cellular homeostasis and recover from damage. Because of their tiny structures and richness in the soma, the detailed morphology of the ER and its dynamics have not been well analysed. In this study, the focused ion beam/scanning electron microscopy (FIB/SEM) analysis was performed to explore the ultra-structures of the ER in the somata of motor neuron with axon regenerative injury models. In normal motor neurons, ER in the somata is abundantly localised near the perinucleus and represents lamella-like structures. After injury, analysis of the ER volume and ER branching points indicated a collapse of the normal distribution and a transformation from lamella-like structures to mesh-like structures. Furthermore, accompanied by ER accumulation near the plasma membrane (PM), the contact between the ER and PM (ER-PM contacts) significantly increased after injury. The accumulation of extended-synaptotagmin 1 (E-Syt1), a tethering protein of the ER and PM that regulates Ca2+-dependent lipid transfer, was also identified by immunohistochemistry and quantitative Real-time PCR after injury. These morphological alterations of ER and the increase in ER-PM contacts may be crucial events that occur in motor neurons as a resilient response for the survival after axonal injury.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"71-84"},"PeriodicalIF":3.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10997708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0