Cell and Tissue Research最新文献_第5页

Correction to: BMP2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells. 更正为BMP2 可诱导干细胞的软骨分化、成骨分化和软骨内骨化。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 DOI: 10.1007/s00441-024-03882-x

Nian Zhou, Qi Li, Xin Lin, Ning Hu, Jun-Yi Liao, Liang-Bo Lin, Chen Zhao, Zhen-Ming Hu, Xi Liang, Wei Xu, Hong Chen, Wei Huang

引用次数: 0

Dynamic relationship among extracellular matrix and body wall cells in Hirudo verbana morphogenesis. 细胞外基质和体壁细胞在马鞭草形态发生中的动态关系

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-03-01 DOI: 10.1007/s00441-024-03874-x

Laura Pulze, Nicolò Baranzini, Francesco Acquati, Gaia Marcolli, Annalisa Grimaldi

{"title":"Dynamic relationship among extracellular matrix and body wall cells in Hirudo verbana morphogenesis.","authors":"Laura Pulze, Nicolò Baranzini, Francesco Acquati, Gaia Marcolli, Annalisa Grimaldi","doi":"10.1007/s00441-024-03874-x","DOIUrl":"10.1007/s00441-024-03874-x","url":null,"abstract":"A great bulk of recent experimental evidence suggests the key role of the complex crosstalk between the extracellular matrix (ECM) and the cellular component of tissues during morphogenesis and embryogenesis. In particular, remodeling of the ECM and of its physical interactions pattern with surrounding cells represent two crucial processes that might be involved in muscle development. However, little information is available on this topic, especially on invertebrate species. To obtain new insights on how tuning the ECM microenvironment might drive cellular fate during embryonic development, we used the invertebrate medicinal leech Hirudo verbana as a valuable experimental model, due to its simple anatomy and the recapitulation of many aspects of the basic biological processes of vertebrates. Our previous studies on leech post-embryonic development have already shown the pivotal role of ECM changes during the growth of the body wall and the role of Yes-associated protein 1 (YAP1) in mechanotransduction. Here, we suggest that the interactions between stromal cell telocytes and ECM might be crucial in driving the organization of muscle layers during embryogenesis. Furthermore, we propose a possible role of the pleiotropic enzyme HvRNASET2 as a possible modulator of collagen deposition and ECM remodeling not only during regenerative processes (as previously demonstrated) but also in embryogenesis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11055932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regional differences in the ultrastructure of mucosal macrophages in the rat large intestine. 大鼠大肠粘膜巨噬细胞超微结构的区域差异。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-03-15 DOI: 10.1007/s00441-024-03883-w

Shota Murase, Youhei Mantani, Nobuhiko Ohno, Asaka Shimada, Satoki Nakanishi, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi

{"title":"Regional differences in the ultrastructure of mucosal macrophages in the rat large intestine.","authors":"Shota Murase, Youhei Mantani, Nobuhiko Ohno, Asaka Shimada, Satoki Nakanishi, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi","doi":"10.1007/s00441-024-03883-w","DOIUrl":"10.1007/s00441-024-03883-w","url":null,"abstract":"We previously clarified the histological characteristics of macrophages in the rat small intestine using serial block-face scanning electron microscopy (SBF-SEM). However, the regional differences in the characteristics of macrophages throughout the large intestine remain unknown. Here, we performed a pilot study to explore the regional differences in the ultrastructure of mucosal macrophages in the large intestine by using SBF-SEM analysis. SBF-SEM analysis conducted on the luminal side of the cecum and descending colon revealed macrophages as amorphous cells possessing abundant lysosomes and vacuoles. Macrophages in the cecum exhibited a higher abundance of lysosomes and a lower abundance of vacuoles than those in the descending colon. Macrophages with many intraepithelial cellular processes were observed beneath the intestinal superficial epithelium in the descending colon. Moreover, macrophages in contact with nerve fibers were more prevalent in the cecum than in the descending colon, and a subset of them surrounded a nerve bundle only in the cecum. In conclusion, the present pilot study suggested that the quantity of some organelles (lysosomes and vacuoles) in macrophages differed between the cecum and the descending colon and that there were some region-specific subsets of macrophages like nerve-associated macrophages in the cecum.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140130828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracrine and endocrine pathways of natriuretic peptides assessed by ligand-receptor mapping in the Japanese eel brain. 通过配体-受体映射评估日本鳗鱼大脑中钠利尿肽的旁分泌和内分泌途径。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-02-19 DOI: 10.1007/s00441-024-03873-y

Tomoki Izumi, Ami Saito, Takanori Ida, Takao Mukuda, Yukitoshi Katayama, Marty Kwok-Shing Wong, Takehiro Tsukada

{"title":"Paracrine and endocrine pathways of natriuretic peptides assessed by ligand-receptor mapping in the Japanese eel brain.","authors":"Tomoki Izumi, Ami Saito, Takanori Ida, Takao Mukuda, Yukitoshi Katayama, Marty Kwok-Shing Wong, Takehiro Tsukada","doi":"10.1007/s00441-024-03873-y","DOIUrl":"10.1007/s00441-024-03873-y","url":null,"abstract":"The natriuretic peptide (NP) family consists of cardiac NPs (ANP, BNP, and VNP) and brain NPs (CNPs) in teleosts. In addition to CNP1-4, a paralogue of CNP4 (named CNP4b) was recently discovered in basal teleosts including Japanese eel. Mammals have lost most Cnps during the evolution, but teleost cnps were conserved and diversified, suggesting that CNPs are important hormones for maintaining brain functions in teleost. The present study evaluated the potency of each Japanese eel CNP to their NP receptors (NPR-A, NPR-B, NPR-C, and NPR-D) overexpressed in CHO cells. A comprehensive brain map of cnps- and nprs-expressing neurons in Japanese eel was constructed by integrating the localization results obtained by in situ hybridization. The result showed that CHO cells expressing NPR-A and NPR-B induced strong cGMP productions after stimulation by cardiac and brain NPs, respectively. Regarding brain distribution of cnps, cnp1 is engaged in the ventral telencephalic area and periventricular area including the parvocellular preoptic nucleus (Pp), anterior/posterior tuberal nuclei, and periventricular gray zone of the optic tectum. cnp3 is found in the habenular nucleus and prolactin cells in the pituitary. cnp4 is expressed in the ventral telencephalic area, while cnp4b is expressed in the motoneurons in the medullary area. Such CNP isoform-specific localizations suggest that function of each CNP has diverged in the eel brain. Furthermore, the Pp lacking the blood-brain barrier expressed both npra and nprb, suggesting that endocrine and paracrine NPs interplay for regulating the Pp functions in Japanese eels.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KDELR2 promotes bone marrow mesenchymal stem cell osteogenic differentiation via GSK3β/β-catenin signaling pathway. KDELR2通过GSK3β/β-catenin信号通路促进骨髓间充质干细胞成骨分化。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-03-12 DOI: 10.1007/s00441-024-03884-9

Xiaoyong Wu, Weijun Zhang, Long Long, Yibo Wang, Hongyu Chen, Kanbin Wang, Zhongxiang Wang, Jinwu Bai, Deting Xue, Zhijun Pan

引用次数: 0

Entry of cannabidiol into the fetal, postnatal and adult rat brain. 大麻二酚进入胎儿、出生后和成年大鼠大脑的情况。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 Epub Date: 2024-02-17 DOI: 10.1007/s00441-024-03867-w

Georgia Fitzpatrick, Yifan Huang, Fiona Qiu, Mark D Habgood, Robert L Medcalf, Heidi Ho, Katarzyna M Dziegielewska, Norman R Saunders

{"title":"Entry of cannabidiol into the fetal, postnatal and adult rat brain.","authors":"Georgia Fitzpatrick, Yifan Huang, Fiona Qiu, Mark D Habgood, Robert L Medcalf, Heidi Ho, Katarzyna M Dziegielewska, Norman R Saunders","doi":"10.1007/s00441-024-03867-w","DOIUrl":"10.1007/s00441-024-03867-w","url":null,"abstract":"Cannabidiol is a major component of cannabis but without known psychoactive properties. A wide range of properties have been attributed to it, such as anti-inflammatory, analgesic, anti-cancer, anti-seizure and anxiolytic. However, being a fairly new compound in its purified form, little is known about cannabidiol brain entry, especially during development. Sprague Dawley rats at four developmental ages: embryonic day E19, postnatal day P4 and P12 and non-pregnant adult females were administered intraperitoneal cannabidiol at 10 mg/kg with [3H] labelled cannabidiol. To investigate the extent of placental transfer, the drug was injected intravenously into E19 pregnant dams. Levels of [3H]-cannabidiol in blood plasma, cerebrospinal fluid and brain were estimated by liquid scintillation counting. Plasma protein binding of cannabidiol was identified by polyacrylamide gel electrophoresis and its bound and unbound fractions measured by ultrafiltration. Using available RNA-sequencing datasets of E19 rat brain, choroid plexus and placenta, as well as P5 and adult brain and choroid plexus, expression of 13 main cannabidiol receptors was analysed. Results showed that cannabidiol rapidly entered both the developing and adult brains. Entry into CSF was more limited. Its transfer across the placenta was substantially restricted as only about 50% of maternal blood plasma cannabidiol concentration was detected in fetal plasma. Albumin was the main, but not exclusive, cannabidiol binding protein at all ages. Several transcripts for cannabidiol receptors were expressed in age- and tissue-specific manner indicating that cannabidiol may have different functional effects in the fetal compared to adult brain.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11055756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139746240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Nescient helix-loop-helix 1 (Nhlh1) is a novel activating transcription factor 5 (ATF5) target gene in olfactory and vomeronasal sensory neurons in mice. 更正：Nescient helix-loop-helix 1 (Nhlh1) 是小鼠嗅觉和绒毛感觉神经元中一种新型激活转录因子 5 (ATF5) 的靶基因。

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-05-01 DOI: 10.1007/s00441-024-03891-w

Chiharu Ishii, Haruo Nakano, Riko Higashiseto, Yusaku Ooki, Mariko Umemura, Shigeru Takahashi, Yuji Takahashi

引用次数: 0

Gadolinium retention effect on macrophages — a potential cause of MRI contrast agent Dotarem toxicity 钆对巨噬细胞的滞留效应--磁共振成像造影剂多他雷毒性的潜在原因

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-16 DOI: 10.1007/s00441-024-03885-8

Marta Halasa, Ahmed Uosef, Henry V. Ubelaker, Arijita Subuddhi, Krupa R. Mysore, Jacek Z. Kubiak, Rafik M. Ghobrial, Jarek Wosik, Malgorzata Kloc

{"title":"Gadolinium retention effect on macrophages — a potential cause of MRI contrast agent Dotarem toxicity","authors":"Marta Halasa, Ahmed Uosef, Henry V. Ubelaker, Arijita Subuddhi, Krupa R. Mysore, Jacek Z. Kubiak, Rafik M. Ghobrial, Jarek Wosik, Malgorzata Kloc","doi":"10.1007/s00441-024-03885-8","DOIUrl":"https://doi.org/10.1007/s00441-024-03885-8","url":null,"abstract":"Gadolinium is a component of the MRI contrast agent Dotarem. Although Dotarem is the least toxic among MRI contrasts used, gadolinium present in Dotarem accumulates for many years in various organs and tissues exerting toxic effects. We showed previously that gadolinium remains in macrophages for at least 7 days after exposure to Dotarem. However, very little is known about the effect of gadolinium retention on the immune cells such as macrophages. We studied the effect of 1-day and 7-day retention of gadolinium on various functions and molecular pathways of macrophages. Gadolinium retention for 7 days decreased macrophage adhesion and motility and dysregulated the expression of adhesion and fibrotic pathway-related proteins such as Notch1 and its ligand Jagged1, adhesion/migration-related proteins PAK1 and Shp1, immune response-related transcription factors Smad3 and TCF19, and chemokines CXCL10 and CXCL13, and dysregulated the mRNA expression of fibrosis-related genes involved in extracellular matrix (ECM) synthesis, such as Col6a1, Fibronectin, MMP9, and MMP12. It also completely (below a level of detection) shut down the transcription of anti-inflammatory M2 macrophage polarization marker the Arg-1. Such changes, if they occur in MRI patients, can be potentially detrimental to the patient’s immune system and immune response-related processes.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140615275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD34+ synovial fibroblasts exhibit high osteogenic potential in synovial chondromatosis CD34+ 滑膜成纤维细胞在滑膜软骨瘤病中表现出很高的成骨潜能

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-11 DOI: 10.1007/s00441-024-03892-9

Xiaoyu Li, Hao Sun, Deng Li, Zhiqing Cai, Jie Xu, Ruofan Ma

{"title":"CD34+ synovial fibroblasts exhibit high osteogenic potential in synovial chondromatosis","authors":"Xiaoyu Li, Hao Sun, Deng Li, Zhiqing Cai, Jie Xu, Ruofan Ma","doi":"10.1007/s00441-024-03892-9","DOIUrl":"https://doi.org/10.1007/s00441-024-03892-9","url":null,"abstract":"Synovial chondromatosis (SC) is a disorder of the synovium characterized by the formation of osteochondral nodules within the synovium. This study aimed to identify the abnormally differentiated progenitor cells and possible pathogenic signaling pathways. Loose bodies and synovium were obtained from patients with SC during knee arthroplasty. Single-cell RNA sequencing was used to identify cell subsets and their gene signatures in SC synovium. Cells derived from osteoarthritis (OA) synovium were used as controls. Multi-differentiation and colony-forming assays were used to identify progenitor cells. The roles of transcription factors and signaling pathways were investigated through computational analysis and experimental verification. We identified an increased proportion of CD34+ sublining fibroblasts in SC synovium. CD34+CD31− cells and CD34−CD31− cells were sorted from SC synovium. Compared with CD34− cells, CD34+ cells had larger alkaline phosphatase (ALP)-stained area and calcified area after osteogenic induction. In addition, CD34+ cells exhibited a stronger tube formation ability than CD34− cells. Our bioinformatic analysis suggested the expression of TWIST1, a negative regulator of osteogenesis, in CD34− sublining fibroblasts and was regulated by the TGF-β signaling pathway. The experiment showed that CD34+ cells acquired the TWIST1 expression during culture and the combination of TGF-β1 and harmine, an inhibitor of Twist1, could further stimulate the osteogenesis of CD34+ cells. Overall, CD34+ synovial fibroblasts in SC synovium have multiple differentiation potentials, especially osteogenic differentiation potential, and might be responsible for the pathogenesis of SC.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140568067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression of neuropeptide F in the digestive organs of female freshwater prawn, Macrobrachium rosenbergii, during the ovarian cycle 雌性淡水对虾消化器官中神经肽 F 在卵巢周期中的差异表达

IF 3.6 3区生物学

Cell and Tissue Research Pub Date : 2024-04-09 DOI: 10.1007/s00441-024-03893-8

Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul

{"title":"Differential expression of neuropeptide F in the digestive organs of female freshwater prawn, Macrobrachium rosenbergii, during the ovarian cycle","authors":"Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul","doi":"10.1007/s00441-024-03893-8","DOIUrl":"https://doi.org/10.1007/s00441-024-03893-8","url":null,"abstract":"Neuropeptide F is a key hormone that controls feeding in invertebrates, including decapod crustaceans. We investigated the differential expression of Macrobrachium rosenbergii neuropeptide F (MrNPF) in the digestive organs of female prawns, M. rosenbergii, during the ovarian cycle. By using RT-qPCR, the expression of MrNPF mRNA in the esophagus (ESO), cardia (CD), and pylorus (PY) of the foregut (FG) gradually increased from stage II and peaked at stage III. In the midgut (MG), hindgut (HG), and hepatopancreas (HP), MrNPF mRNA increased from stage I, reaching a maximal level at stage II, and declined by about half at stages III and IV (P < 0.05). In the ESO, CD, and PY, strong MrNPF-immunoreactivities were seen in the epithelium, muscle, and lamina propria. Intense MrNPF-ir was found in the MG cells and the muscular layer. In the HG, MrNPF-ir was detected in the epithelium of the villi and gland regions, while MrNPF-ir was also more intense in the F-, R-, and B-cells in the HP. However, we found little colocalization between the MrNPF and PGP9.5/ChAT in digestive tissues, implying that most of the positive cells might not be neurons but could be digestive tract-associated endocrine cells that produce and secrete MrNPF to control digestive organ functions in feeding and utilizing feed. Taken together, our first findings indicated that MrNPF was differentially expressed in digestive organs in correlation with the ovarian cycle, suggesting an important link between MrNPF, the physiology of various digestive organs in feeding, and possibly ovarian maturation in female M. rosenbergii.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140568157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0