Cell and Tissue Research最新文献_第4页

Folliculogenesis and follicular growth in the endangered fish Brycon opalinus, with emphasis on ovarian matrix remodeling. 濒危鱼类卵泡发生和卵泡生长，重点是卵巢基质重塑。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-19 DOI: 10.1007/s00441-025-03982-2

Camila Ferreira Sales, Bruna Aquino Araújo, Sarah Morais de Souza, Elizete Rizzo, Rafael Magno Costa Melo

{"title":"Folliculogenesis and follicular growth in the endangered fish Brycon opalinus, with emphasis on ovarian matrix remodeling.","authors":"Camila Ferreira Sales, Bruna Aquino Araújo, Sarah Morais de Souza, Elizete Rizzo, Rafael Magno Costa Melo","doi":"10.1007/s00441-025-03982-2","DOIUrl":"https://doi.org/10.1007/s00441-025-03982-2","url":null,"abstract":"Ovarian development of fishes involves significant functional and structural changes during the process of mature egg formation. In this study, we report the folliculogenesis and follicular growth in the endangered species Brycon opalinus as well as the dynamics of the proteins Foxl2, Col4 and MMP-9 during ovarian maturation. Seventeen females were collected in the Doce River basin and their ovaries were subjected to histological techniques, electron microscopy, immunohistochemistry and Western blotting. In ovaries of B. opalinus, isolated oogonia and oogonia grouped in niches were identified, both surrounded by prefollicle cells. Primary growth initiated when the ovarian follicle was formed by the oocyte and follicle cells, in addition to the formation of zona radiata. Secondary growth was characterized by the incorporation of yolk into the oocyte and development of the zona radiata and follicle cells. Proportion of oogonia, perinucleolar follicles, and ovarian stroma was higher during resting stage, while previtellogenic and vitellogenic follicles were more frequent in the early and advanced maturation stages. Foxl2 was expressed mainly in prefollicle cells in oogonia and follicle cells in young follicles during reproductive resting. Col4 was expressed in basement membrane and extracellular matrix, primarily during resting and early maturation of ovaries. MMP-9 showed discrete labeling in ovarian matrix, but theca cells and zona radiata of secondary growth follicles, as well as granulocytes in matrix, showed significant immunolabeling during advanced maturation. We conclude that during ovarian development of B. opalinus there is intense participation and interaction of Foxl2, Col4 and MMP-9 with matrix components, somatic and germ cells for formation and follicular growth.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures. 大分子添加剂对星形胶质细胞细胞外基质沉积的影响。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-19 DOI: 10.1007/s00441-025-03980-4

Sorour Nemati, Michelle Kilcoyne, Dimitrios Zeugolis, Siobhan S McMahon

{"title":"The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures.","authors":"Sorour Nemati, Michelle Kilcoyne, Dimitrios Zeugolis, Siobhan S McMahon","doi":"10.1007/s00441-025-03980-4","DOIUrl":"https://doi.org/10.1007/s00441-025-03980-4","url":null,"abstract":"Macromolecular crowding (MMC) is a biophysical phenomenon that has proven effective in enhancing extracellular matrix (ECM) deposition in vitro. However, MMCs potential in neuroglial cell cultures remains underexplored. This study investigates the effects of three distinct MMC agents [carrageenan (CR), dextran sulphate (DxS) and FicollⓇ cocktail (FC)] on ECM deposition and cell behaviour of Neu7 and primary astrocytes. While the viability and metabolic activity of Neu7 astrocytes were unaffected by any of the crowding agents, primary astrocytes exhibited a significant decrease in viability and metabolic activity in the presence of CR and DxS. The addition of CR, DxS, and FC resulted in a significant increase in deposition of fibronectin, collagen IV, collagen I, GFAP and CS56 in Neu7 astrocytes. In primary astrocytes, FC significantly enhanced the expression of astrocytic markers and increased the deposition of ECM proteins, including fibronectin and collagen IV. This study highlights the advantages of using FC as a MMC agent for enhancing ECM deposition in astrocytes. The method demonstrates potential for developing fast and more physiologically relevant in vitro models and improving drug screening processes for future studies. The observed benefits underscore the utility of FC in creating advanced cellular models that better mimic the native neural environment.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A historical perspective of Kupffer cells in the context of infection. 从历史角度看感染背景下的 Kupffer 细胞。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-01 Epub Date: 2024-10-11 DOI: 10.1007/s00441-024-03924-4

Carolyn T Graham, Siamon Gordon, Paul Kubes

引用次数: 0

Roots and early routes of neuroendocrinology. 神经内分泌学的根源和早期途径。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-01 Epub Date: 2025-01-30 DOI: 10.1007/s00441-024-03928-0

Esteban M Rodríguez, Montserrat Guerra, Juan Luis Blázquez

{"title":"Roots and early routes of neuroendocrinology.","authors":"Esteban M Rodríguez, Montserrat Guerra, Juan Luis Blázquez","doi":"10.1007/s00441-024-03928-0","DOIUrl":"10.1007/s00441-024-03928-0","url":null,"abstract":"Carl C. Speidel (1919) and Ernst Scharrer (1928) were privileged witnesses of the encounter between neurons and hormones, a biological phenomenon that had been occurring in nature during millions of years of evolution, as Berta Scharrer started to unfold since 1935 on. The story of neurosecretion is intimately associated to that of the hypothalamus, such a \"marvellous region\", as Wolfgang Bargmann (1975) called it. This story started more than two millennia ago. We have made an effort to trace the roots of the discoveries that gave rise to a medical discipline, neuroendocrinology. Our trip to the roots covers a period from the fourth century BC, when an extraordinary Medical School was founded in Alexandria, and extends into the late 1970s of the twentieth century, when neuroendocrine research had started to grow exponentially. An effort has been made to track back the origin of each piece of knowledge that was constructing, brick upon brick, the building of this new medical science, hoping that it would help neuroendocrinologists of the new era to find their own roots, to meet their ancestors. Tracking the roots of a particular phenomenon provides the opportunity to have an overview of the whole phenomenon, allowing comprehension rather than merely knowledge. An important purpose pursued throughout this article was to pay a tribute to all those who, in the early days, contributed to the brain-endocrine encounter. We have tried our best to bring back the achievements of most of them.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"137-215"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special issue 100th anniversary Cell and Tissue Research. 《细胞与组织研究》100周年特刊。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-01 DOI: 10.1007/s00441-025-03972-4

Horst-Werner Korf, David P Kelsell, Vera Kozjak-Pavlovic

引用次数: 0

100 years Cell and Tissue Research: the founders and their successors. 100年细胞和组织研究：创始人和他们的继任者。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-05-01 Epub Date: 2025-01-27 DOI: 10.1007/s00441-025-03950-w

Klaus Unsicker

引用次数: 0

Retraction Note to: Preconditioning with melatonin improves therapeutic outcomes of bone marrow-derived mesenchymal stem cells in targeting liver fibrosis induced by CCl4. 注：褪黑素预处理可改善骨髓间充质干细胞靶向CCl4诱导的肝纤维化的治疗效果。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 DOI: 10.1007/s00441-025-03959-1

Keywan Mortezaee, Neda Khanlarkhani, Fatemeh Sabbaghziarani, Saeid Nekoonam, Jamal Majidpoor, Amir Hosseini, Parichehr Pasbakhsh, Iraj Ragerdi Kashani, Adib Zendedel

引用次数: 0

Gross anatomy of the visual processing centers of Hieroglyphus banian. 猴形猿视觉加工中心的大体解剖。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-02-12 DOI: 10.1007/s00441-025-03956-4

Sivaraju C, Joby Joseph

引用次数: 0

The role and mechanism of Schwann cells in the repair of peripheral nerve injury. 雪旺细胞在周围神经损伤修复中的作用及机制。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-02-15 DOI: 10.1007/s00441-025-03957-3

Huiyue Xu, Zhipeng Fan

{"title":"The role and mechanism of Schwann cells in the repair of peripheral nerve injury.","authors":"Huiyue Xu, Zhipeng Fan","doi":"10.1007/s00441-025-03957-3","DOIUrl":"10.1007/s00441-025-03957-3","url":null,"abstract":"Limb injuries such as severe strains, deep cuts, gunshot wounds, and ischemia can cause peripheral nerve damage. This can result in a range of clinical symptoms including sensory deficits, limb paralysis and atrophy, neuralgia, and sweating abnormalities in the innervated areas affected by the damaged nerves. These symptoms can have a significant impact on patients' daily lives and work. Despite existing clinical treatments, some patients cannot achieve satisfactory therapeutic effects and continue to experience persistent paralysis and pain. Schwann cells are responsible for repairing and regenerating damaged nerves in the peripheral nervous system. They play a crucial role in the healing of nerve injuries and are essential for the restoration of proper nerve function. An increasing number of studies have focused on the various regulatory mechanisms that specifically affect the repair of damage by Schwann cells. This article aims to provide information on the different types of peripheral nerve injuries and their available treatments. We also discuss the various molecular mechanisms that regulate Schwann cell function during peripheral nerve repair and how they can be used to promote nerve repair and regeneration. Furthermore, we explore the potential therapeutic applications of precision regulation of Schwann cells for the treatment of peripheral nerve injuries.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"81-95"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recruitment of pulmonary intravascular macrophages in SARS-CoV-2 infected hamsters. SARS-CoV-2感染仓鼠肺血管内巨噬细胞的募集

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-04-01 Epub Date: 2025-02-27 DOI: 10.1007/s00441-025-03958-2

Carolina Rego Rodrigues, Gurpreet Kaur Aulakh, Andrea Kroeker, Swarali S Kulkarni, Jocelyne Lew, Darryl Falzarano, Baljit Singh

{"title":"Recruitment of pulmonary intravascular macrophages in SARS-CoV-2 infected hamsters.","authors":"Carolina Rego Rodrigues, Gurpreet Kaur Aulakh, Andrea Kroeker, Swarali S Kulkarni, Jocelyne Lew, Darryl Falzarano, Baljit Singh","doi":"10.1007/s00441-025-03958-2","DOIUrl":"10.1007/s00441-025-03958-2","url":null,"abstract":"The mechanisms by which severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe lung inflammation and mortality remain unclear. While the role of alveolar macrophages in COVID-19 is known, data on pulmonary intravascular macrophages (PIMs) is lacking. PIMs are key inflammatory cells present in species like cattle and pigs. Though constitutively absent in humans and rodents, their recruitment in rodents triggers exaggerated inflammation. We investigated the recruitment of PIMs and other immune cells, using immunofluorescence, hematoxylin and eosin (H&E) staining, and immunogold labeling in a hamster model of SARS-CoV-2 infection. Syrian golden hamsters were divided into 6 groups: uninfected control, unvaccinated-infected at 2-, 5-, and 14-days post infection (dpi) and vaccinated-infected at 5- and 14-dpi. Lung tissues were analyzed for neutrophils (myeloperoxidase), monocytes/macrophages (CCR2, CX3CR1), macrophages (IBA-1), and T cells (CD3). Septal macrophages increased at 2-, 5-, and 14-dpi in infected animals vs. control. CX3CR1 + cells decreased at 14-dpi in unvaccinated animals, but CX3CR1/CCR2 double positive cells were higher at 5-dpi, indicating a pro-inflammatory macrophage phenotype. PIMs were confirmed by transmission electron microscopy. These are the first data showing recruitment of pro-inflammatory PIMs in SARS-CoV-2 infected lungs.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"1-15"},"PeriodicalIF":3.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0