Cell and Tissue Research最新文献_第4页

Impact of hydroxyapatite nanoparticles on the cellular processes of stem cells derived from dental tissue sources. 羟基磷灰石纳米颗粒对牙组织来源干细胞细胞过程的影响。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-18 DOI: 10.1007/s00441-025-03962-6

Mais Emad, Mohammad Alnatour, Walhan Alshaer, Jennifer L Gibbs, Benoît Michot, Dana Alqudah, Alaa A A Aljabali, Mairvat Al-Mrahleh, Abdolelah Jaradat, Duaa Abuarqoub

{"title":"Impact of hydroxyapatite nanoparticles on the cellular processes of stem cells derived from dental tissue sources.","authors":"Mais Emad, Mohammad Alnatour, Walhan Alshaer, Jennifer L Gibbs, Benoît Michot, Dana Alqudah, Alaa A A Aljabali, Mairvat Al-Mrahleh, Abdolelah Jaradat, Duaa Abuarqoub","doi":"10.1007/s00441-025-03962-6","DOIUrl":"https://doi.org/10.1007/s00441-025-03962-6","url":null,"abstract":"Hydroxyapatite nanoparticle (HANPs) utilization has recently been notable in bone tissue engineering. This surge owes itself to the biocompatibility of HANPs and their striking resemblance to the minerals found in natural bone. Furthermore, dental pulp-derived stem cells (DPSCs) have garnered attention due to their remarkable differentiation potential into multilineages, thus positioning them as a pivotal cell reservoir for regenerative medicine. This study aims to investigate the impact of HANPs on DPSCs cellular processes. The HANPs have been synthesized using the wet chemical precipitation method followed by freeze-drying and characterization using dynamic light scattering (DLS) and transmission electron microscopy (TEM). The size of HANPs was reported to be in the range of 55-67 nm. Our dataset divulges that DPSCs can endure concentrations of HANPs up to ≤ 0.81 mg/mL without incurring any conspicuous alterations in their morphology or the pace of proliferation. Furthermore, the self-renewal potency of HANPs was upheld at concentrations ≤ 0.20 mg/mL. Flow cytometric analysis affirms a significant divergence in cell distribution across all cell cycle phases in DPSCs treated with 0.81 mg/mL HANPs. Intriguingly, no variance surfaced in the migratory capacity of DPSCs exposed to HANPs of ≤ 0.40 mg/mL. For osteogenic differentiation, HANPs at concentrations of ≤ 0.40 mg/mL demonstrated the aptitude to incite osteogenic differentiation within DPSCs, facilitating the formation of calcium deposits. In conclusion, combining HANPs and DPSCs shows promise for restoring damaged hard tissues, like bone and teeth, and enhancing regenerative therapies.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Microtubule organization and tubulin post‑translational modifications in intact tissues and during regeneration in calcareous sponges. 对完整组织和钙质海绵再生过程中微管组织和微管蛋白翻译后修饰的修正。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-14 DOI: 10.1007/s00441-025-03964-4

Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov

引用次数: 0

Long-term consumption of moderate amounts of sucrose-sweetened drinks disrupts intestinal barrier function by impairing goblet cell differentiation. 长期饮用适量的含糖饮料会损害杯状细胞的分化，从而破坏肠道屏障功能。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-12 DOI: 10.1007/s00441-025-03961-7

Sachiko Sato, Arif U Hasan, Mami Obara, Yukiko Kondo, Eiichi Taira

{"title":"Long-term consumption of moderate amounts of sucrose-sweetened drinks disrupts intestinal barrier function by impairing goblet cell differentiation.","authors":"Sachiko Sato, Arif U Hasan, Mami Obara, Yukiko Kondo, Eiichi Taira","doi":"10.1007/s00441-025-03961-7","DOIUrl":"https://doi.org/10.1007/s00441-025-03961-7","url":null,"abstract":"While the prolonged consumption of sucrose-containing beverages is known to impact many organs, their specific effects on the small intestine remain elusive. This study aimed to evaluate how regular intake of sucrose, in amounts typically consumed, affects goblet cells, which play a critical role in regulating the mucosal barrier and innate immune defenses in the small intestine. Ten-week-old male ddY mice, a model of diet-induced obesity, were given a regular diet with either plain water or 7% sucrose water. Caloric intake was monitored weekly through food and drink measurements. After 8 weeks, glucose and insulin responses were evaluated following an oral gavage of glucose or sucrose. At 14 weeks, plasma, whole small intestine, and liver samples were collected. Despite achieving an isocaloric state, mice drinking sucrose water showed approximately a 1.5-fold increase in body weight and impaired glucose tolerance. In the small intestine, genes involved in sucrose digestion and absorption (Sis, Sglt1, Glut2, and Glut5) were upregulated, while genes essential for maintaining the intestinal barrier and function (Epcam, Fabp2, Cldn1, Ocln, and Tjp1) were downregulated. Serum levels and mRNA expression of the inflammatory cytokine, interleukin-18 were elevated. Genes responsible for goblet cell differentiation and function (Hes1, Gfi1, Spdef, and Klf4) were downregulated, leading to an increase in immature goblet cells and a decrease in mucin-producing markers (Muc2, Muc4, and Muc13) in the jejunum. The findings underscore that besides obesity, long-term intake of sucrose-containing drinks provokes localized inflammation and disrupts small intestinal barrier function by impairing goblet cell differentiation and activity.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease. 1型糖尿病供者和有无胰岛细胞自身抗体的非糖尿病供者胰岛中谷胱甘肽过氧化物酶-1免疫反应细胞的分布是可变的，与疾病无关。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-10 DOI: 10.1007/s00441-025-03955-5

Kaaj Pala, Kevin Xueying Sun, Lars Krogvold, Knut Dahl-Jørgensen, Shiva Reddy

{"title":"Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease.","authors":"Kaaj Pala, Kevin Xueying Sun, Lars Krogvold, Knut Dahl-Jørgensen, Shiva Reddy","doi":"10.1007/s00441-025-03955-5","DOIUrl":"https://doi.org/10.1007/s00441-025-03955-5","url":null,"abstract":"During type 1 diabetes (T1D), oxidative stress in beta cells may cause early beta cell dysfunction and initiate autoimmunity. Mouse islets express lower levels of reactive oxygen species (ROS) clearing enzymes, such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase than several other tissues. It remains unclear if human beta cells show a similar deficiency during T1D or exhibit a higher degree of intrinsic resistance to oxidative stress. We compared islet cell distributions and determined graded intensities of glutathione peroxidase1 (GPX1), a key enzymatic mediator involved in detoxifying hydrogen peroxide, by applying combined immunohistochemistry for GPX1, insulin and glucagon, in pancreatic sections from new-onset T1D (group 1), non-diabetic autoantibody-negative (group 2), non-diabetic autoantibody-positive (group 3) and long-term diabetic (group 4) donors. Islets from all study groups demonstrated either uniform but graded staining intensities for GPX1 in almost all islet cells or strong staining in selective islet cells with weaker intensities in the remaining cells. GPX1 was present in selective glucagon cells and insulin cells, including in cells negative for both hormones, with stronger intensities in a higher percentage of glucagon than insulin cells. It was absent in a higher percentage of beta cells than glucagon cells independent of disease or autoantibody positivity. We conclude that a proportion of human beta cells and glucagon cells express GPX1 but show heterogeneity in its distribution and intensities, independent of disease or autoantibody status. Our studies highlight important differences in the expression of GPX1 in islet cell-types between mice and humans.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges. 钙质海绵中完整组织和再生过程中的微管组织和微管蛋白翻译后修饰。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-05 DOI: 10.1007/s00441-025-03960-8

Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov

{"title":"Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges.","authors":"Kseniia V Skorentseva, Fyodor V Bolshakov, Aleena A Saidova, Andrey I Lavrov","doi":"10.1007/s00441-025-03960-8","DOIUrl":"10.1007/s00441-025-03960-8","url":null,"abstract":"Microtubules are the principal cytoskeletal component in cells, integral to various morphogenetic processes in Metazoa, including cell migration, adhesion, and polarity. Their dynamics and functions are modulated by tubulin post-translational modifications (PTMs). While studies on model species have provided insights into microtubule functions, understanding their evolutionary aspects necessitates exploring non-model organisms. Sponges (phylum Porifera) are an early-branching metazoan group with outstanding regenerative capacities. This research presents the first comprehensive analysis of microtubule organization and tubulin PTMs in calcareous sponges. The intact sponge cells show various but typical types of microtubule organization, while detected tubulin PTMs are associated with certain cell types, indicating specific functions in particular cellular contexts. During regeneration, relying on the coordinated movement of epithelial-like cell sheets, microtubule networks in exopinacocytes and choanocytes undergo significant reorganization. These rearranged microtubules potentially stabilize cellular migration direction and facilitate cargo transport, essential for cell contact and polarity establishment. This study enhances our understanding of microtubule functionality and regulation in early-diverging metazoans, contributing to the broader evolutionary context of cytoskeletal dynamics.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DjsoxP-1 and Djsox5 are essential for tissue homeostasis and regeneration in Dugesia japonica. DjsoxP-1和Djsox5对杜鹃花的组织稳态和再生至关重要。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1007/s00441-024-03939-x

Weiyun Guo, Xiao Liu, Lina Pang, Zhihong Kong, Ziyi Lin, Jing Ren, Zimei Dong, Guangwen Chen, Dezeng Liu

{"title":"DjsoxP-1 and Djsox5 are essential for tissue homeostasis and regeneration in Dugesia japonica.","authors":"Weiyun Guo, Xiao Liu, Lina Pang, Zhihong Kong, Ziyi Lin, Jing Ren, Zimei Dong, Guangwen Chen, Dezeng Liu","doi":"10.1007/s00441-024-03939-x","DOIUrl":"10.1007/s00441-024-03939-x","url":null,"abstract":"Sox genes encode a family of transcription factors that regulate multiple biological processes during metazoan development, including embryogenesis, tissue homeostasis, nervous system specification, and stem cell maintenance. The planarian Dugesia japonica contains a reservoir of stem cells that grow and divide continuously to support cellular turnover. However, whether SOX proteins retain these conserved functions in planarians remains to be determined. In this study, three sox gene homologs, DjsoxP-1, DjsoxP-5, and Djsox5, were identified in the planarian transcriptome, and their roles were investigated. The results showed that the amino acids deduced from the three sox genes all contained high-mobility group (HMG) domain sequences, which are highly conserved in sox family members. Whole-mount in situ hybridization (WISH) and real-time quantitative PCR (RT-qPCR) results indicated that the three sox genes were mainly expressed in parenchymal tissues and regenerative blastema. Additionally, X-ray irradiation assay and dFISH suggested that the three Djsox genes were expressed in neoblasts and other cell types. Head regression in intact planarian and smaller blastemas in both head or tail fragments of regenerating planarians were exhibited with DjsoxP-1 and Djsox5 RNA interference (RNAi) compared to the control animals, suggesting that DjsoxP-1 and Djsox5 have essential roles during cellular turnover and regeneration in planarians; conversely, there was no obvious phenotypic abnormalities or regeneration defect in DjsoxP-5 RNAi animals. Knockdown of DjsoxP-1 or Djsox5 decreased neoblast proliferation and promoted cell apoptosis. In conclusion, our findings demonstrate that DjsoxP-1 and Djsox5 are involved in cellular turnover and regeneration in planarians by modulating coordination between cell proliferation and apoptosis.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":"399 3","pages":"337-350"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics. 大鼠垂体中间叶的CD9/ sox2阳性细胞表现出间充质干细胞的特征。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-14 DOI: 10.1007/s00441-024-03947-x

Ayano Shindo, Morio Azuma, Ken Fujiwara, Saishu Yoshida, Kotaro Horiguchi

{"title":"CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics.","authors":"Ayano Shindo, Morio Azuma, Ken Fujiwara, Saishu Yoshida, Kotaro Horiguchi","doi":"10.1007/s00441-024-03947-x","DOIUrl":"10.1007/s00441-024-03947-x","url":null,"abstract":"Adult tissue stem cells of the anterior pituitary gland, CD9/SOX2-positive cells, are believed to exist in the marginal cell layer (MCL) bordering the residual lumen of the Rathke's pouch. These cells migrate from the intermediate lobe side of the MCL (IL-MCL) to the anterior lobe side of the MCL and may be involved in supplying hormone-producing cells. Previous studies reported that some SOX2-positive cells of the anterior lobe differentiate into skeletal muscle cells. These findings suggest that CD9/SOX2-positive cells in the anterior pituitary have mesenchymal stem cell (MSC) properties. To substantiate this hypothesis, we examined whether CD9-positive cells isolated from IL-MCL of adult male rats differentiate into mesenchymal cells, such as endothelial cells, adipocytes, chondrocytes, and osteocytes. Immunohistochemical analysis revealed that the CD9-positive cells were positive for the MSC markers, CD349, CD105, CD271, and CD273 and were detected in the early postnatal period at the boundary between the posterior and intermediate lobes but not in the embryonic period. In addition, some adult tissue stem cells derived from neural crest cells and bone marrow haematopoietic stem cells were positive for both CD9 and MSC markers, indicating that several CD9/SOX2-positive cells in the IL-MCL of the pituitary gland are MSCs that invaded from external tissues during pituitary development in the early postnatal period and exist in the adult tissue stem cells as suppliers of hormone-producing and endothelial cells in the anterior lobe. These findings should have implications for the application of CD9/SOX2-positive cells in regenerative therapy of the pituitary.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"277-290"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and histochemical characteristics of the foregut, midgut, and hindgut, and their alterations during ovarian development in female freshwater prawn, Macrobrachium rosenbergii. 罗氏沼虾雌虾前、中、后肠形态和组织化学特征及其在卵巢发育过程中的变化。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-13 DOI: 10.1007/s00441-024-03948-w

Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul

{"title":"Morphological and histochemical characteristics of the foregut, midgut, and hindgut, and their alterations during ovarian development in female freshwater prawn, Macrobrachium rosenbergii.","authors":"Warinthip Vetkama, Ruchanok Tinikul, Prasert Sobhon, Yotsawan Tinikul","doi":"10.1007/s00441-024-03948-w","DOIUrl":"10.1007/s00441-024-03948-w","url":null,"abstract":"The anatomical, histological, and histochemical characteristics of the foregut (FG), midgut (MG), and hindgut (HG), as well as their alterations during the ovarian cycle in female prawns, Macrobrachium rosenbergii, were investigated. The esophagus (ESO), cardia (CD), and pylorus (PY) are the main components of the FG. An epithelium (Ep) with thick cuticle (Cu) layers lining the ESO, and the ESO is encircled by the ESO glands. The CD has a thick musculature, whereas the Ep of the PY are characterized by numerous villi and columnar Ep cells with a thinner layer of Cu. The inner longitudinal (LM) and the outer circular (CM) muscles were both present in the PY. The MG is lined by Ep cells which are connected to the basement membrane, and it lacks Cu. Microvilli, and subapical vacuoles are visible on the apical surface of Ep cells of the MG. The outermost layer is characterized by a dense strip of elastic fibers and a cluster of collagen fibers. The HG has the Ep cells with a thin Cu layer, and the HG glands form a rosette-like structure. The HG is surrounded by the CM and the LM fibers. The reactivities of Periodic Acid Schiff and Alcian Blue in these digestive organs altered throughout the ovarian cycle, and this was supported by the increased expression of mucin levels as ovarian maturation progressed. Our results offer novel and significant insights into the anatomical and histochemical structures of these digestive organs, and demonstrate a significant correlation between ovarian development and feeding in the female prawn, M. rosenbergii.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"351-375"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis. 淡水小龙虾造血组织的组织与表达分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-04 DOI: 10.1007/s00441-024-03943-1

Thanapong Kruangkum, Kenneth Söderhäll, Irene Söderhäll

{"title":"The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis.","authors":"Thanapong Kruangkum, Kenneth Söderhäll, Irene Söderhäll","doi":"10.1007/s00441-024-03943-1","DOIUrl":"10.1007/s00441-024-03943-1","url":null,"abstract":"The hematopoietic tissue (HPT) and anterior proliferation center (APC) are the main hemocyte-producing organs of the freshwater crayfish, Pacifastacus leniusculus. To deepen our understanding of immune responses to various pathogens, it is essential to identify distinct hemocyte subpopulations with specific functions and to further explore how these cells are generated. Here we provide an in-depth histological study of the HPT and APC in order to localize cell types in different developmental stages, and to provide some information regarding the hemocyte differentiation in the crayfish. We localized mRNA expression of previously identified genes in the HPT/APC and hemocytes by RNA-FISH. The expression of hemolectin and transglutaminase 1 was shown to be co-localized in a high number of the HPT cells, while transglutaminase 2 was expressed in different cell types mainly associated with epithelium or endothelium. Furthermore, by double RNA-FISH for hemolectin and a previously unidentified PDGF-like factor, combined with immunostaining for prophenoloxidase, we could identify several different subtypes of hemocytes, indicating that the immune function of hemocytes in crayfish is more diversified and complex than previously appreciated.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"303-322"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic memory as crucial contributing factor in directing the differentiation of human iPSC into pancreatic β-cells in vitro. 表观遗传记忆是体外诱导人iPSC向胰腺β细胞分化的重要因素。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-01-30 DOI: 10.1007/s00441-025-03952-8

Abdoulaye Diane, Razik Bin Abdul Mu-U-Min, Heba Hussain Al-Siddiqi

{"title":"Epigenetic memory as crucial contributing factor in directing the differentiation of human iPSC into pancreatic β-cells in vitro.","authors":"Abdoulaye Diane, Razik Bin Abdul Mu-U-Min, Heba Hussain Al-Siddiqi","doi":"10.1007/s00441-025-03952-8","DOIUrl":"10.1007/s00441-025-03952-8","url":null,"abstract":"Impaired insulin secretion contributes to the pathogenesis of type 1 diabetes mellitus through autoimmune destruction of pancreatic β-cells and the pathogenesis of severe forms of type 2 diabetes mellitus through β-cell dedifferentiation and other mechanisms. Replenishment of malfunctioning β-cells via islet transplantation has the potential to induce long-term glycemic control in the body. However, this treatment option cannot widely be implemented in clinical due to healthy islet donor shortage. Emerging β-cell replacement with human-induced pluripotent stem cell (iPSC) provides high remedial therapy hopes. Thus, tremendous progress has been made in developing β-cell differentiation protocols in vitro; however, most of the differentiated iPSC-derived β-cells showed immature phenotypes associated with low efficiency depending on the iPSC lines used, creating a crucial barrier for their clinical implementation. Multiple mechanisms including differences in genetic, cell cycle patterns, and mitochondrial dysfunction underlie the defective differentiation propensity of iPSC into insulin-producing β-cells. Accumulating evidence recently indicated that, following the reprogramming, epigenetic memory inherited from parental cells substantially affects the differentiation capacity of many iPSC lines. Therefore, differences in epigenetic signature are likely to be essential contributing factors influencing the propensity of iPSC differentiation. In this review, we will document the impact of the epigenome on the reprogramming efficacy and differentiation potential of iPSCs and how targeting the epigenetic residual memory could be an additional strategy to improve the differentiation efficiency of existing protocols to generate fully functional hPSC-derived pancreatic β-cells for diabetes therapy and drug screening.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"267-276"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0