Cell and Tissue Research最新文献_第4页

Local inflammation at the salmon louse (Lepeophtheirus salmonis) attachment site contributes to copepodid rejection in coho salmon (Oncorhynchus kisutch). 鲑鱼虱（Lepeophtheirus salmonis）附着部位的局部炎症有助于鲑（Oncorhynchus kisutch）的桡足类排斥反应。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 Epub Date: 2025-06-04 DOI: 10.1007/s00441-025-03976-0

Lene Sveen, Mark D Fast, Torstein Tengs, Rachel A Kline, Judit Aguilar Marti, Dominic Kurian, Gerrit Timmerhaus, Marianne Vaadal, Ross D Houston, James E Bron, Sean J Monaghan, Haitham H Mohammed, Rose Ruiz Daniels, Sarah Salisbury, Diego Robledo, Mark Braceland, Miroslava Hansen, Nicholas Robinson

{"title":"Local inflammation at the salmon louse (Lepeophtheirus salmonis) attachment site contributes to copepodid rejection in coho salmon (Oncorhynchus kisutch).","authors":"Lene Sveen, Mark D Fast, Torstein Tengs, Rachel A Kline, Judit Aguilar Marti, Dominic Kurian, Gerrit Timmerhaus, Marianne Vaadal, Ross D Houston, James E Bron, Sean J Monaghan, Haitham H Mohammed, Rose Ruiz Daniels, Sarah Salisbury, Diego Robledo, Mark Braceland, Miroslava Hansen, Nicholas Robinson","doi":"10.1007/s00441-025-03976-0","DOIUrl":"10.1007/s00441-025-03976-0","url":null,"abstract":"The study investigates the susceptibility of Atlantic salmon (Salmo salar) and Pacific salmon species (pink salmon, Oncorhynchus gorbuscha; coho salmon, Oncorhynchus kisutch; and chum salmon, Oncorhynchus keta) to the parasitic salmon lice (Lepeophtheirus salmonis). The research had two main objectives: to characterize the morphology of the scaly skin in four salmonid species and to compare the cellular response at the louse attachment site in coho salmon and Atlantic salmon. Three consecutive challenge trials were conducted, with successful louse infestation only achieved across all four species in the third trial using mild anesthesia with tricaine methanesulfonate. Skin and fin samples were collected at 12, 24, 36, 48, 60, and 168 h post-infestation (hpi) for histological, proteomic, and spatial transcriptomic analyses. Results showed that chum salmon had significantly higher mucous cell coverage (30-40%) in the epithelium of scaly skin compared to Atlantic salmon (10%). At the louse attachment site in coho salmon, there was a greater influx of inflammatory cells at 36-48 hpi compared to Atlantic salmon. Proteomic analysis at 12 hpi and 36 hpi in coho salmon showed upregulation of neutrophil degranulation and formyl-methionyl-leucyl-phenylalanine signaling. Additionally, spatial transcriptomics at the attachment site showed local upregulation of inflammatory gene markers. These findings suggest that coho salmon mount a rapid and large-scale inflammatory response driven by neutrophils to louse attachment within the first 48 hpi. Overall, the study emphasizes the significance of local changes at the host-parasite interface for resistance mechanisms against salmon lice.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"181-211"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12325551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue-specific differences impacts therapeutic outcomes of mitochondrial transplantation through regulation of bioenergetics in metabolic syndrome. 组织特异性差异通过调节代谢综合征的生物能量学影响线粒体移植的治疗结果。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 Epub Date: 2025-05-26 DOI: 10.1007/s00441-025-03977-z

Jyotsna Pareek, Pallavi Mudgal, Nitika Sindhu, Vaibhav Tiwari, Dinesh Mani Tripathi, Swati Paliwal

{"title":"Tissue-specific differences impacts therapeutic outcomes of mitochondrial transplantation through regulation of bioenergetics in metabolic syndrome.","authors":"Jyotsna Pareek, Pallavi Mudgal, Nitika Sindhu, Vaibhav Tiwari, Dinesh Mani Tripathi, Swati Paliwal","doi":"10.1007/s00441-025-03977-z","DOIUrl":"10.1007/s00441-025-03977-z","url":null,"abstract":"Mitochondria transplantation is an emerging therapeutic strategy with remarkable potential in treating various diseases associated with mitochondrial dysfunction. Despite the known differences in tissue-specific mitochondria, the therapeutic outcomes of mitochondria isolated from various sources, after their transplantation in a specific disease model has remained elusive. In this study, we investigated the tissue-dependent therapeutic differences after transplantation of mitochondria isolated from heart, muscle, and liver tissues in a high-fat diet and streptozotocin, 35 mg/Kg (HFD + STZ) induced metabolic syndrome (MetS) in Wistar rats. We found striking differences in lowering of blood glucose levels, blood pressure, cholesterol, ALT, and AST levels in MetS after transplantation of mitochondria obtained from heart, muscle, and liver tissues (P < 0.01). Liver mitochondria transplantation demonstrated the most effective upregulation of mitochondrial complex activities, enhanced anti-oxidant enzyme levels in recipient liver tissues (P < 0.01). It also upregulated gene expression of genes associated with mitochondrial biogenesis and bioenergetics and reduced apoptosis and inflammation associated genes in HFD + STZ rats. In addition, GC-MS metabolite analysis revealed differential blood serum concentrations of key tri-carboxylic acid metabolites such as succinic acid, malic acid, alpha-ketoglutarate, citric acid, and pyruvate after mitochondrial transplantation in HFD + STZ rats. This study supports the idea that mitochondria source tissue should be considered to provide better clinical outcomes for mitochondrial transplantation.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"213-228"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization management of acute phase increases healing ligament strength. 急性期的固定治疗可增加愈合韧带的强度。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 Epub Date: 2025-05-23 DOI: 10.1007/s00441-025-03983-1

Chiharu Takasu, Sora Kawabata, Hidenobu Terada, Takuma Kojima, Yuri Morishita, Yuichiro Oka, Kiyomi Takayanagi, Naohiko Kanemura, Kenji Murata

{"title":"Immobilization management of acute phase increases healing ligament strength.","authors":"Chiharu Takasu, Sora Kawabata, Hidenobu Terada, Takuma Kojima, Yuri Morishita, Yuichiro Oka, Kiyomi Takayanagi, Naohiko Kanemura, Kenji Murata","doi":"10.1007/s00441-025-03983-1","DOIUrl":"10.1007/s00441-025-03983-1","url":null,"abstract":"Conservative treatment of the anterior cruciate ligament (ACL) is important for restoring functional activity and preventing secondary degeneration. However, the molecular mechanisms underlying ligament immobilization and its precise role in the healing process remain poorly understood. In this study, we investigated the effect of immobilization on the strength of the healed ACL during acute management. We performed surgery to heal the ACL of rats and immobilized the knees using Kirschner wires. The group in which only the surgery to promote ACL healing was performed was designated as the controlled anterior tibial instability group, whereas the group that underwent both surgery and immobilization was designated as the immobilization (IMM) group. After 1-2 weeks of immobilization, histological analyses using hematoxylin-eosin staining and immunohistochemical evaluation of collagen types I and III expression were performed. A comprehensive genetic analysis in the acute phase was performed via RNA sequencing. Furthermore, fibroblasts derived from rat ACL were used to recapitulate inflammation with interleukin-1β, and its effect on elongation stress (110%) was investigated using polymerase chain reaction. Joint immobilization for 2 weeks postoperatively increased the mechanical strength of the conservatively connected ligaments. Stretch stimulation of fibroblasts with interleukin-1β also decreased the expression of the extracellular matrix. Furthermore, bioinformatics analyses identified differentially expressed genes associated with the healing process in fixed versus unfixed ligaments. The results demonstrate that acute-phase immobilization, defined as fixation for 2 weeks following injury, enhances ligament strength by promoting extracellular matrix synthesis and organized regeneration, providing novel insights into optimizing conservative ACL therapy.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"155-165"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hsa-MiR-483 -3p regulates the extracellular matrix proteins via TGFβ2/SMAD4 signaling in the glucocorticoid-responsive human trabecular meshwork cells. 在糖皮质激素应答的人小梁网细胞中，Hsa-MiR-483 -3p通过tgf - β2/SMAD4信号通路调节细胞外基质蛋白。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 Epub Date: 2025-06-05 DOI: 10.1007/s00441-025-03985-z

Ravinarayanan Haribalaganesh, Rajendrababu Sharmila, Ramasamy Krishnadas, Colin E Willoughby, Srinivasan Senthilkumari

{"title":"Hsa-MiR-483 -3p regulates the extracellular matrix proteins via TGFβ2/SMAD4 signaling in the glucocorticoid-responsive human trabecular meshwork cells.","authors":"Ravinarayanan Haribalaganesh, Rajendrababu Sharmila, Ramasamy Krishnadas, Colin E Willoughby, Srinivasan Senthilkumari","doi":"10.1007/s00441-025-03985-z","DOIUrl":"10.1007/s00441-025-03985-z","url":null,"abstract":"The purpose of this study was to investigate the role of hsa-miR-483-3p on the regulation of extracellular matrix (ECM) in cultured human trabecular meshwork (HTM) cells with known steroid response. Primary cultures of HTM cells with known steroid responsiveness [GC-responder (GC-R) and GC-Non-responder (GC-NR) cells] were grown on coverslip in 12-well plate until 80% confluence and treated with 100 nM dexamethasone (DEX) for 24 h and transfected with different concentrations of synthetic miRNA 483-3p mimic or inhibitor. After 24 h or 72 h post transfection, the cells were harvested for the following experiments: (i) percentage transfection efficiency, (ii) RNA isolation for qPCR analysis, (iii) immunofluorescence staining, and (iv) protein isolation for Western blotting, respectively. All experiments were performed in triplicate with three biological samples (n = 3). GC-R HTM cells showed significantly higher expression of SMAD4 as compared to GC-NR HTM cells. Similarly, DEX treatment up-regulated the SMAD4-dependent ECM proteins. The presence of a miR-483-3p mimic down-regulated SMAD4 expression and SMAD4-dependent ECM production in a dose-dependent manner by negatively down-regulating SMAD4/TGF-β2 signaling. The inhibition of SMAD4-dependent ECM production by miR-483-3p was more pronounced in GC-R HTM cells as compared to GC-NR cells. The down-regulation in ECM production by miR-483-3p is SMAD4-dependent and may play a protective role in mitigating steroid response in GC-R HTM cells. Using miR-483-3p mimics demonstrates therapeutic potential for the management of steroid-induced ocular hypertension and glaucoma.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"145-154"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures. 大分子添加剂对星形胶质细胞细胞外基质沉积的影响。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 Epub Date: 2025-05-19 DOI: 10.1007/s00441-025-03980-4

Sorour Nemati, Michelle Kilcoyne, Dimitrios Zeugolis, Siobhan S McMahon

{"title":"The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures.","authors":"Sorour Nemati, Michelle Kilcoyne, Dimitrios Zeugolis, Siobhan S McMahon","doi":"10.1007/s00441-025-03980-4","DOIUrl":"10.1007/s00441-025-03980-4","url":null,"abstract":"Macromolecular crowding (MMC) is a biophysical phenomenon that has proven effective in enhancing extracellular matrix (ECM) deposition in vitro. However, MMCs potential in neuroglial cell cultures remains underexplored. This study investigates the effects of three distinct MMC agents [carrageenan (CR), dextran sulphate (DxS) and FicollⓇ cocktail (FC)] on ECM deposition and cell behaviour of Neu7 and primary astrocytes. While the viability and metabolic activity of Neu7 astrocytes were unaffected by any of the crowding agents, primary astrocytes exhibited a significant decrease in viability and metabolic activity in the presence of CR and DxS. The addition of CR, DxS, and FC resulted in a significant increase in deposition of fibronectin, collagen IV, collagen I, GFAP and CS56 in Neu7 astrocytes. In primary astrocytes, FC significantly enhanced the expression of astrocytic markers and increased the deposition of ECM proteins, including fibronectin and collagen IV. This study highlights the advantages of using FC as a MMC agent for enhancing ECM deposition in astrocytes. The method demonstrates potential for developing fast and more physiologically relevant in vitro models and improving drug screening processes for future studies. The observed benefits underscore the utility of FC in creating advanced cellular models that better mimic the native neural environment.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"129-143"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12325430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Sertoli cell-conditioned medium restores spermatogenesis in azoospermic mouse testis. 更正：支持细胞条件培养基恢复无精子小鼠睾丸中的精子发生。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 DOI: 10.1007/s00441-025-03986-y

Sakineh Panahi, Amin Karamian, Ensieh Sajadi, Abbas Aliaghaei, Hamid Nazarian, Shabnam Abdi, Samira Danyali, Shahrokh Paktinat, Mohammad-Amin Abdollahifar, Reza Mastery Farahani

引用次数: 0

Correction to: Transplantation of R-GSIK scaffold with mesenchymal stem cells improves neuroinflammation in a traumatic brain injury model. 更正：移植间充质干细胞的R-GSIK支架可改善创伤性脑损伤模型的神经炎症。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 DOI: 10.1007/s00441-025-03991-1

Sajad Sahab Negah, Mohammad Moein Shirzad, Ghazale Biglari, Farzin Naseri, Hassan Hosseini Ravandi, Ali Hassani Dooghabadi, Ali Gorji

引用次数: 0

Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice. calbindin D-28K神经元性别差异的出现及calbindin D-28K在小鼠视前区聚类神经元中的作用。

IF 2.9 3区生物学

Cell and Tissue Research Pub Date : 2025-08-01 Epub Date: 2025-05-21 DOI: 10.1007/s00441-025-03981-3

Ryoma Koiso, Chihiro Kushida, Moeko Kanaya, Shinji Tsukahara

{"title":"Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice.","authors":"Ryoma Koiso, Chihiro Kushida, Moeko Kanaya, Shinji Tsukahara","doi":"10.1007/s00441-025-03981-3","DOIUrl":"10.1007/s00441-025-03981-3","url":null,"abstract":"Neurons expressing Calb1 that encodes calbindin D-28K (Calb), a calcium-binding protein, are significant components of the sexually dimorphic nucleus (SDN) of the preoptic area in mice. Calb is therefore used as a marker to study the SDN. The number of Calb neurons in the SDN is greater in males than in females, and this sex difference emerges before puberty. However, the timing of emergence of this difference and the role of Calb1 in the SDN remain unclear. In this study, we investigated when the sex difference in Calb neurons appears and whether Calb1 is required for organizing the SDN. Profiling the temporal changes in Calb neurons revealed that these neurons dramatically increased in the SDN during postnatal days 15 to 20 in males but not in females, resulting in a significant difference between sexes. However, the Calb1 mRNA in the SDN remained unchanged during the late postnatal period and did not differ between sexes. Calb1 knockdown (KD) was performed by injecting an adeno-associated virus vector into the preoptic area of neonatal mice. The analysis of postnatal Calb1-KD mice revealed that Calb1 KD reduced not only Calb1 expression but also the number of neurons comprising the SDN. These findings suggest that compared with the female SDN, more Calb neurons migrate to and are incorporated into the male SDN, thereby yielding the sex difference in the number of Calb neurons at the late postnatal period, and that Calb1 plays a significant role in clustering neurons in the SDN.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"117-128"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12325559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection. COVID-19急性感染患者呼吸肌组织病理学分析。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-05-05 DOI: 10.1007/s00441-025-03973-3

Laura Steingruber, Simona Handtke, Franziska Schweiger, Stefan Schiele, Bruno Märkl, Marco Koch

{"title":"Histopathological analysis of respiratory muscles in patients with acute COVID-19 infection.","authors":"Laura Steingruber, Simona Handtke, Franziska Schweiger, Stefan Schiele, Bruno Märkl, Marco Koch","doi":"10.1007/s00441-025-03973-3","DOIUrl":"10.1007/s00441-025-03973-3","url":null,"abstract":"Coronavirus-disease 2019 (COVID-19) affects the respiratory system with high morbidity in elderly and comorbid patients. Acute COVID-19 infection (CI) primarily leads to respiratory failure, long-term effects on respiratory skeletal muscle however remain vague. Thus, histopathological marker expression of oxidative stress, inflammation, satellite cell activity, myosin fiber composition, and cellular senescence were analyzed in intercostal muscle and diaphragm to compare respiratory muscle degeneration (RMD) in deceased CI-positive and control patients. Beside CI, the impact of BMI, age, sex, ventilation status, and duration of hospitalization on RMD were evaluated. CI-positive patients exhibited higher numbers of regenerative stem cells, but no association between CI status and RMD was observed. Interestingly, ventilation support and lung-associated comorbidities had no effect on expression of RMD markers (p > 0.05). However, intercostal muscle showed BMI-dependent changes in expression of RMD markers, regardless of the CI status, with increased cytokine expression (p = 0.04), reduced antioxidative capacity (p = 0.05), and low stem cell prevalence (p = 0.02) in patients with high BMI. Moreover, elderly patients demonstrated increased oxidative stress (p = 0.001) and cell senescence (p = 0.03) independent of CI status. Notably, immobility drives muscle fiber transformation to Myosin ST (p = 0.03), since prolonged hospitalization correlated with muscle fiber type shift. Limitations included incomplete retrospective data collection and absence of adequate samples for molecular analyses. Together, RMD is influenced by BMI, age and immobility rather than the CI status alone. Future studies including larger cohorts, molecular analyses, and evaluation of patient data in addition to CI status alone, will further support meaningful analyses and interpretation of RMD and its impact on post CI recovery.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"59-68"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liquid-liquid phase separation in normal hematopoiesis and hematological diseases. 液-液相分离在正常造血和血液系统疾病中的应用。

IF 3.2 3区生物学

Cell and Tissue Research Pub Date : 2025-07-01 Epub Date: 2025-05-17 DOI: 10.1007/s00441-025-03974-2

Lexuan Lin, Fang He, Xu Jin, Xiaoru Zhang, Yue Li, Di Wang, Jingwei Wang, Lingyue Zheng, Haoze Song, Xu Zhu, Qimei Cheng, Yitong Zhao, Jing Liang, Jinfa Ma, Jie Gao, Jingyuan Tong, Lihong Shi

{"title":"Liquid-liquid phase separation in normal hematopoiesis and hematological diseases.","authors":"Lexuan Lin, Fang He, Xu Jin, Xiaoru Zhang, Yue Li, Di Wang, Jingwei Wang, Lingyue Zheng, Haoze Song, Xu Zhu, Qimei Cheng, Yitong Zhao, Jing Liang, Jinfa Ma, Jie Gao, Jingyuan Tong, Lihong Shi","doi":"10.1007/s00441-025-03974-2","DOIUrl":"10.1007/s00441-025-03974-2","url":null,"abstract":"Liquid-liquid phase separation (LLPS) is an emerging research field in cellular biology. LLPS-driven biomolecular condensates act as reaction chambers and regulatory hubs for critical processes, including chromatin architecture, gene expression, and metabolism. The dysregulation of these processes frequently impedes the proper execution of physiological functions. Current research indicates that abnormal phase separation plays a significant role in the pathogenesis of diseases and aging. This review briefly overviews the fundamental concepts and research methods related to phase separation. We also summarize studies concerning its physiological functions, particularly emphasizing its role in hematopoiesis. We further discuss how abnormal phase separation can lead to hematological disorders, specifically summarizing its involvement in the pathogenesis of leukemia. Despite recent advancements, elucidating LLPS mechanisms in hematopoiesis remains challenging due to the intricate interplay between biomolecular condensates and cellular function. Future research efforts aiming to reveal the role of LLPS in hematological diseases hold promise for novel therapeutic interventions and a deeper understanding of hematopoietic processes.","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"15-27"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0