埃及伊蚊内分泌腺复合体的发育变化。

IF 2.9 3区 生物学 Q3 CELL BIOLOGY
Lenka Rouhová, Gabriela Krejčová, Ana Beatriz Barletta Ferreira, Houda Ouns Maaroufi, Fernando G Noriega, Carolina Barillas-Mury, Hana Sehadová, Marcela Nouzova
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引用次数: 0

摘要

在埃及伊蚊的幼虫中,三种最重要的内分泌腺,即腹体(CA)、心体(CC)和前胸腺(PG)共同构成腺体复合物(GC)。利用共聚焦激光扫描显微镜结合免疫组织化学、原位杂交、超微结构扩增显微镜和细胞凋亡研究,我们能够识别GC的不同细胞类型,并跟踪它们在变态过程中的命运。我们的研究表明,CC不是一个定义明确的器官,而是由随机分布在GC和CA-CC复合物中的单个细胞组成。此外,成像和原位杂交显示CA是由单一细胞类型组成的致密器官。我们观察到CA和CC在幼虫到成虫的转变过程中存活,而PG在成虫生命的前24小时内凋亡并消失。蚊子是几种重要传染病的传播媒介,本研究为更详细地了解蚊子主要内分泌器官的结构和变化奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Developmental changes in the Aedes aegypti mosquito endocrine gland complex.

In the larvae of the mosquito Aedes aegypti, the three most important endocrine glands, the corpora allata (CA), the corpora cardiaca (CC), and the prothoracic gland (PG), together form the glandular complexes (GC). Using confocal laser scanning microscopy in combination with immunohistochemistry, in situ hybridization, ultrastructural expansion microscopy, and apoptosis studies, we were able to identify the different cell types of the GC and follow their fate during metamorphosis. Our studies revealed that the CC is not a well-defined organ but consists of individual cells randomly distributed within the GC and CA-CC complexes. Furthermore, imaging and in situ hybridization show that the CA is a compact organ composed of a single cell type. We observed that CA and CC survive during the larval-to-adult transition, while PG undergoes apoptosis and disappears within the first 24 h of adult life. This study lays the foundation for a more detailed understanding of the structure and changes in the major endocrine organs of mosquitoes, which are vectors of several important infectious diseases.

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来源期刊
Cell and Tissue Research
Cell and Tissue Research 生物-细胞生物学
CiteScore
7.00
自引率
2.80%
发文量
142
审稿时长
1 months
期刊介绍: The journal publishes regular articles and reviews in the areas of molecular, cell, and supracellular biology. In particular, the journal intends to provide a forum for publishing data that analyze the supracellular, integrative actions of gene products and their impact on the formation of tissue structure and function. Submission of papers with an emphasis on structure-function relationships as revealed by recombinant molecular technologies is especially encouraged. Areas of research with a long-standing tradition of publishing in Cell & Tissue Research include: - neurobiology - neuroendocrinology - endocrinology - reproductive biology - skeletal and immune systems - development - stem cells - muscle biology.
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