Cell Adhesion & Migration最新文献

{"title":"Dynamized ultra-low dilution of <i>Ruta graveolens</i> disrupts plasma membrane organization and decreases migration of melanoma cancer cell.","authors":"Camille Fuselier, Eleonore Dufay, Alexandre Berquand, Christine Terryn, Arnaud Bonnomet, Michael Molinari, Laurent Martiny, Christophe Schneider","doi":"10.1080/19336918.2022.2154732","DOIUrl":"10.1080/19336918.2022.2154732","url":null,"abstract":"<p><p>Cutaneous melanoma is a cancer with a very poor prognosis mainly because of metastatic dissemination and therefore a deregulation of cell migration. Current therapies can benefit from complementary medicines as supportive care in oncology. In our study, we show that a dynamized ultra-low dilution of <i>Ruta Graveolens</i> leads to an <i>in vitro</i> inhibition of migration on fibronectin of B16F10 melanoma cells, as well as a decrease in metastatic dissemination <i>in vivo</i>. These effects appear to be due to a disruption of plasma membrane organization, with a change in cell and membrane stiffness, associated with a disorganization of the actin cytoskeleton and a modification of the lipid composition of the plasma membrane. Together, these results demonstrate, in <i>in vitro</i> and <i>in vivo</i> models of cutaneous melanoma, an anti-cancer and anti-metastatic activity of ultra-low dynamized dilution of <i>Ruta graveolens</i> and reinforce its interest as complementary medicine in oncology.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"17 1","pages":"1-13"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10657273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling cell migration: defining movement from the cell surface.","authors":"Francisco Merino-Casallo, Maria Jose Gomez-Benito, Silvia Hervas-Raluy, Jose Manuel Garcia-Aznar","doi":"10.1080/19336918.2022.2055520","DOIUrl":"10.1080/19336918.2022.2055520","url":null,"abstract":"<p><p>Cell motility is essential for life and development. Unfortunately, cell migration is also linked to several pathological processes, such as cancer metastasis. Cells' ability to migrate relies on many actors. Cells change their migratory strategy based on their phenotype and the properties of the surrounding microenvironment. Cell migration is, therefore, an extremely complex phenomenon. Researchers have investigated cell motility for more than a century. Recent discoveries have uncovered some of the mysteries associated with the mechanisms involved in cell migration, such as intracellular signaling and cell mechanics. These findings involve different players, including transmembrane receptors, adhesive complexes, cytoskeletal components , the nucleus, and the extracellular matrix. This review aims to give a global overview of our current understanding of cell migration.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"16 1","pages":"25-64"},"PeriodicalIF":3.3,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41877505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Class I Myosins, molecular motors involved in cell migration and cancer.","authors":"Juan D Diaz-Valencia,&nbsp;Laura A Estrada-Abreo,&nbsp;Leonor Rodríguez-Cruz,&nbsp;Alfonso R Salgado-Aguayo,&nbsp;Genaro Patiño-López","doi":"10.1080/19336918.2021.2020705","DOIUrl":"https://doi.org/10.1080/19336918.2021.2020705","url":null,"abstract":"<p><p>Class I Myosins are a subfamily of motor proteins with ATPase activity and a characteristic structure conserved in all myosins: A N-Terminal Motor Domain, a central Neck and a C terminal Tail domain. Humans have eight genes for these myosins. Class I Myosins have different functions: regulate membrane tension, participate in endocytosis, exocytosis, intracellular trafficking and cell migration. Cell migration is influenced by many cellular components including motor proteins, like myosins. Recently has been reported that changes in myosin expression have an impact on the migration of cancer cells, the formation of infiltrates and metastasis. We propose that class I myosins might be potential markers for future diagnostic, prognostic or even as therapeutic targets in leukemia and other cancers.<b>Abbreviations:</b> Myo1g: Myosin 1g; ALL: Acute Lymphoblastic Leukemia, TH1: Tail Homology 1; TH2: Tail Homology 2; TH3: Tail Homology 3.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"16 1","pages":"1-12"},"PeriodicalIF":3.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10255785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluid shear stress induces cell migration via RhoA-YAP1-autophagy pathway in liver cancer stem cells.","authors":"Zhiping Yan,&nbsp;Danfeng Guo,&nbsp;Ruolin Tao,&nbsp;Xiao Yu,&nbsp;Jiacheng Zhang,&nbsp;Yuting He,&nbsp;Jiakai Zhang,&nbsp;Jie Li,&nbsp;Shuijun Zhang,&nbsp;Wenzhi Guo","doi":"10.1080/19336918.2022.2103925","DOIUrl":"https://doi.org/10.1080/19336918.2022.2103925","url":null,"abstract":"<p><p>Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC), but the role of the RhoA-YAP1-autophagy pathway in HCC remains unclear. Due to the core role of liver cancer stem cells (LCSCs) in HCC metastasis and recurrence, we explored the RhoA-YAP1-autophagy pathway in LCSCs under FSS. Our results indicate that LCSCs have stronger proliferation and cell spheroidization abilities. FSS (1 dyn/cm²) upregulated the migration of LCSCs and autophagy protein markers, inducing LC3B aggregation and autophagosome formation in LCSCs. Mechanistically, FSS promoted YAP1 dephosphorylation and transport to the nucleus, which is mediated by RhoA, inducing autophagy. Finally, inhibition of autophagy suppressed cell migration in LCSCs under FSS. In conclusion, FSS promoted the migration of LCSCs via the RhoA-YAP1-autophagy pathway.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"16 1","pages":"94-106"},"PeriodicalIF":3.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/92/d5/KCAM_16_2103925.PMC9331214.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10623073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of GRP78 ATPase suppresses A549 lung cancer cell migration by promoting ITGB4 degradation.","authors":"Junya Ning,&nbsp;Xiaoling Cui,&nbsp;Nan Li,&nbsp;Na Li,&nbsp;Baoxiang Zhao,&nbsp;Junying Miao,&nbsp;Zhaomin Lin","doi":"10.1080/19336918.2022.2130415","DOIUrl":"https://doi.org/10.1080/19336918.2022.2130415","url":null,"abstract":"<p><p>Hypochlorous acid (HOCl) is an essential signal molecule in cancer cells. Activated GRP78 ATPase by a HOCl probe named ZBM-H inhibits lung cancer cell growth. However, the role and underlying mechanism of GRP78 ATPase in lung cancer cell migration have not been established. Here, we reported that activation of GRP78 ATPase by ZBM-H suppressed A549 cell migration and inhibited EMT process. Notably, ZBM-H time-dependently decreased the protein level of integrin β4 (ITGB4) in A549 cells. Combinatorial treatment of 3BDO (an autophagy inhibitor) and ZBM-H partially rescued the protein level of ITGB4. Consistently, 3BDO partially reversed ZBM-H-inhibited cell migration. Furthermore, ZBM-H promoted the interaction between ANXA7 and Hsc70, which participated in the regulation of selective autophagy and degradation of ITGB4.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"16 1","pages":"107-114"},"PeriodicalIF":3.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10637614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel role of Dipterocarpus tuberculatus as a stimulator of focal cell adhesion through the regulation of MLC2/FAK/Akt signaling pathway","authors":"Su jin Lee, Ji Eun Kim, J. W. Jung, Y. Choi, J. Gong, Bounleuane Douangdeuane, Onevilay Souliya, Y. Choi, Sung Baek Seo, D. Hwang","doi":"10.1080/19336918.2022.2073002","DOIUrl":"https://doi.org/10.1080/19336918.2022.2073002","url":null,"abstract":"ABSTRACT To investigate a novel function of Dipterocarpus tuberculatus on focal cell adhesion stimulation, alterations to the regulation of focal cell adhesion-related factors were analyzed in NHDF cells and a calvarial defect rat model after treatment with methanol extracts of D. tuberculatus (MED). MED contained gallic acid, caffeic acid, ellagic acid, and naringenin in high concentrations. The proliferation activity, focal cell adhesion ability, adhesion receptors-mediated signaling pathway in NHDF cells were increased by MED. Also, a dense adhered tissue layer and adherent cells on MED-coated titanium plate (MEDTiP) surfaces were detected during regeneration of calvarial bone. The results of the present study provide novel evidence that MED may stimulate focal cell adhesion in NHDF cells and a calvarial defect rat model.","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"16 1","pages":"72 - 93"},"PeriodicalIF":3.2,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46930438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triple-negative and triple-positive breast cancer cells reciprocally control their growth and migration via the S100A4 pathway","authors":"E. Dukhanina, T. Portseva, A. Dukhanin, S. Georgieva","doi":"10.1080/19336918.2022.2072554","DOIUrl":"https://doi.org/10.1080/19336918.2022.2072554","url":null,"abstract":"ABSTRACT The study’s aim was to investigate the S100A4-mediated mechanisms of the regulation of tumor cell proliferation and migration in the human triple-positive breast carcinoma cell line MCF-7 (TPBC) and triple-negative breast carcinoma cell line MDA-MB-231 (TNBC). The proliferative activity of TNBC more than doubled during the incubation in the conditioned medium of TPBC. Extracellular S100A4 dose-dependently decreased the proliferative response of TPBC. TPBC negatively impacted the growth of TNBCs during their co-culturing. TPBC significantly decreased the migration activity of the TNBC cells while the S100A4 intracellular level in the TNBC was also decreasing. The decrease in the S100A4 intracellular level occurred due to the protein’s monomeric form while the contribution of the dimeric form into the overall S100A4 concentration in TNBC cells increased 1.5-2-fold. The S100A4 pathway in the intercellular communication between TNBC and TPBCs also included the dexamethasone-sensitive mechanisms of S100A4 intra- and extracellular pools regulation.","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"16 1","pages":"65 - 71"},"PeriodicalIF":3.2,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49213300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of exosome-derived microRNAs reveals insights of intercellular communication during invasion of breast, prostate and glioblastoma cancer cells.","authors":"Francesca Lessi,&nbsp;Paolo Aretini,&nbsp;Milena Rizzo,&nbsp;Mariangela Morelli,&nbsp;Michele Menicagli,&nbsp;Sara Franceschi,&nbsp;Chiara Maria Mazzanti","doi":"10.1080/19336918.2021.1935407","DOIUrl":"10.1080/19336918.2021.1935407","url":null,"abstract":"<p><p>MiRNAs represent a mechanism that regulates gene expression in many pathological conditions. Exosomes are known to be secreted from all types of cells, and the exosomes-released molecules are crucial messengers that can regulate cellular processes. We investigated the miRNAs content of exosomes released by cancer cells during the invasion . An invasion stimulus has been generated through scratches created on the confluent cells of cancer cell lines: glioblastoma, breast and prostate cancers.Several miRNAs were found to be significantly differentially abundant during the cell invasion , both in common among different cell lines and exclusive. Understanding the language codes among cells involved in invasion can lead to the development of therapies that can inhibit cellular communication, slowing or eventually stopping their activity.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"15 1","pages":"180-201"},"PeriodicalIF":3.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336918.2021.1935407","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39097492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of laminin and cognate receptors in cholangiocarcinoma cell migration.","authors":"Kittiya Islam,&nbsp;Parichut Thummarati,&nbsp;Pakkanun Kaewkong,&nbsp;Banchob Sripa,&nbsp;Tuangporn Suthiphongchai","doi":"10.1080/19336918.2021.1924422","DOIUrl":"https://doi.org/10.1080/19336918.2021.1924422","url":null,"abstract":"<p><p>Extensive desmoplasia in cholangiocarcinoma (CCA) is associated with tumor aggressiveness, indicating a need for further understanding of CCA cell-matrix interaction. This study demonstrated laminin as the most potent attractant for CCA cell migration and the vast elevation of its receptor integrin β4 (ITGB4) in CCA cell lines. Besides, their high expressions in CCA tissues were correlated with lymphatic invasion and the presence of ITGB4 was also associated with short survival time. ITGB4 silencing revealed it as the receptor for laminin-induced HuCCA-1 migration, but KKU-213 utilized 37/67-kDa laminin receptor (LAMR) instead. These findings highlight the role of ITGB4 and LAMR in transducing laminin induction of CCA cell migration and the potential of ITGB4 as diagnostic and prognostic biomarkers for CCA.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"15 1","pages":"152-165"},"PeriodicalIF":3.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336918.2021.1924422","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39000750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"P21-activated kinase regulates oxygen-dependent migration of vascular endothelial cells in monolayers.","authors":"Satomi Hirose,&nbsp;Yugo Tabata,&nbsp;Kazuki Sone,&nbsp;Naoyuki Takahashi,&nbsp;Daisuke Yoshino,&nbsp;Kenichi Funamoto","doi":"10.1080/19336918.2021.1978368","DOIUrl":"https://doi.org/10.1080/19336918.2021.1978368","url":null,"abstract":"<p><p>The collective migration of vascular endothelial cells plays important roles in homeostasis and angiogenesis. Oxygen concentration <i>in vivo</i>, which is lower than in the atmosphere and changes due to diseases, is a key factor affecting the cellular dynamics of vascular endothelial cells. We previously reported that hypoxic conditions promote the internalization of vascular endothelial (VE)-cadherin, a specific cell-cell adhesion molecule, and increase the velocity of the collective migration of vascular endothelial cells. However, the mechanism through which cells regulate collective migration as affected by oxygen tension is not fully understood. Here, we investigated oxygen-dependent collective migration, focusing on intracellular protein p21-activated kinase (PAK) and hypoxia-inducing factor (HIF)-1α. A monolayer of human umbilical vein vascular endothelial cells (HUVECs) was formed in a microfluidic device with controllability of oxygen tension. The HUVECs were then exposed to various oxygen conditions in a range from 0.8% to 21% O₂, with or without PAK inhibition or chemical stabilization of HIF-1α. Collective cell migration was measured by particle image velocimetry with time-lapse phase-contrast microscopic images. Localizations of VE-cadherin and HIF-1α were quantified by immunofluorescent staining. The collective migration of HUVECs varied in an oxygen-dependent fashion; the migration speed was increased by hypoxic exposure down to 1% O₂, while it decreased under an extremely low oxygen tension of less than 1% O₂. PAK inhibition suppressed the hypoxia-induced increase of the migration speed by preventing VE-cadherin internalization into HUVECs. A decrease in the migration speed was also obtained by chemical stabilization of HIF-1α, suggesting that excessive accumulation of HIF-1α diminishes collective cell migration. These results indicate that the oxygen-dependent variation of the migration speed of vascular endothelial cells is mediated by the regulation of VE-cadherin through the PAK pathway, as well as other mechanisms via HIF-1α, especially under extreme hypoxic conditions.</p>","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"15 1","pages":"272-284"},"PeriodicalIF":3.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39437794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}