Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer.

IF 3.3 3区生物学 Q3 CELL BIOLOGY

Cell Adhesion & Migration Pub Date : 2024-12-01 Epub Date: 2024-04-01 DOI:10.1080/19336918.2024.2335881

Lei Zhang, Wenbang Chen, Xiaojun Li, Gengming Wang, Fubao Xing, Xiao Zhu

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引用次数: 0

Abstract

We aimed to investigate galectin-1 overexpression induces normal fibroblasts (NFs) translates into cancer-associated fibroblasts (CAFs). Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell. The motilities of H1299 and A549 cells were measured. Human umbilical vein endothelial cell (HUVEC) proliferation and tube formation ability were assessed. Tumor volume and tumor weight was recorded. Cells motilities were increased, while apoptosis rates were decreased after CMs co-cultured. B-cell lymphoma-2 (Bcl-2) expression level was increased, while Bcl2-associatedX (Bax) and cleaved-caspase3 decreased. CMs treatment enhanced HUVEC proliferation and tube formation. Tumor volume and weight in CMs treated mice were increased, and the sensitivity of anlotinib in co-cultured cells was decreased. Our results revealed that galectin-1 overexpression induced NFs translated into CAFs.

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Galectin-1过表达可诱导正常成纤维细胞转化为癌症相关成纤维细胞，并降低肺癌患者对安罗替尼的敏感性。

我们的目的是研究 galectin-1 过表达会诱导正常成纤维细胞（NFs）转化为癌症相关成纤维细胞（CAFs）。在人胚胎肺成纤维细胞（HFL1）中过表达 Galectin-1。测量了 H1299 和 A549 细胞的运动能力。评估人脐静脉内皮细胞（HUVEC）的增殖和管形成能力。记录肿瘤体积和重量。共培养 CMs 后，细胞运动能力增强，而细胞凋亡率降低。B细胞淋巴瘤-2（Bcl-2）表达水平升高，而Bcl2相关X（Bax）和裂解-caspase3表达水平降低。CMs处理可促进HUVEC增殖和管形成。经 CMs 处理的小鼠肿瘤体积和重量增加，共培养细胞对安罗替尼的敏感性降低。我们的研究结果表明，galectin-1过表达会诱导NFs转化为CAFs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Adhesion & Migration CELL BIOLOGY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

53 weeks

期刊介绍： Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.