Copine C plays a role in adhesion and streaming in Dictyostelium.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cell Adhesion & Migration Pub Date : 2024-12-01 Epub Date: 2024-02-20 DOI:10.1080/19336918.2024.2315629
Rodney A Nichols, Amber D Ide, Cody T Morrison, Amber L Anger, Matthew J Buccilli, Cynthia K Damer
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引用次数: 0

Abstract

Copines are a family of calcium-dependent membrane-binding proteins. To study these proteins, anull mutant for cpnC was created in Dictyostelium, which has six copines genes (cpnA-cpnF). During development, cpnC- cells were able to aggregate, but did not form streams. Once aggregated into mounds, they formed large ring structures. cpnC- cells were less adherent to plastic substrates, but more adherent to other cells. These phenotypes correlated with changes in adhesion protein expression with decreased expression of SibA and increased expression of CsaA in developing cpnC- cells. We also measured the expression of RegA, a cAMP phosphodiesterase, and found that cpnC- cells have reduced RegA expression. The reduced RegA expression in cpnC- cells is most likely responsible for the observed phenotypes.

Copine C 在竹荪的粘附和流变过程中发挥作用。
共价键是钙依赖性膜结合蛋白的一个家族。为了研究这些蛋白,我们在竹荪中创建了一个 cpnC 空突变体,该突变体有六个 copines 基因(cpnA-cpnF)。在发育过程中,cpnC-细胞能够聚集,但不能形成流。cpnC- 细胞对塑料基质的粘附性较低,但对其他细胞的粘附性较高。这些表型与粘附蛋白表达的变化有关,在发育中的 cpnC- 细胞中,SibA 的表达量减少,而 CsaA 的表达量增加。我们还测量了 cAMP 磷酸二酯酶 RegA 的表达,发现 cpnC- 细胞的 RegA 表达减少。cpnC- 细胞中 RegA 表达的减少很可能是导致观察到的表型的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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