Cell Adhesion & Migration最新文献_第10页

The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium 人脂肪组织产生的细胞因子对单核细胞粘附内皮的影响

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2019-01-01 DOI: 10.1080/19336918.2019.1644856

S. Cejkova, H. Kubátová, F. Thieme, L. Janousek, J. Fronek, R. Poledne, I. Králová Lesná

{"title":"The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium","authors":"S. Cejkova, H. Kubátová, F. Thieme, L. Janousek, J. Fronek, R. Poledne, I. Králová Lesná","doi":"10.1080/19336918.2019.1644856","DOIUrl":"https://doi.org/10.1080/19336918.2019.1644856","url":null,"abstract":"ABSTRACT Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro‑inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were tested using adipose tissue-conditioned media (ATCM) prepared by culturing human VAT. The cytokines concentrations in ATCM, the cytokines expression and adhesion molecules in stimulated ECs were measured. The concentrations of IL-1β,TNF-α,MCP-1,IL-10,and RANTES measured in ATCM correlated positively with monocyte adhesiveness to ECs. Additionally, ATCM increased the adhesion molecules (ICAM-1, VCAM-1) gene expression. Selective inhibitors highlighted the importance of IL-1β and TNF-α in the process by a significant decrease in monocyte adhesion compared to ATCM preconditioning without inhibitors. Human VAT significantly increased monocyte adhesion to ECs. It was significantly influenced by IL-1β, TNF-α, MCP-1, IL-10, and RANTES, with IL-1β and TNF‑α having the strongest impact.","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336918.2019.1644856","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43935771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Splicing factor-modulated generation of mechano growth factor regulates physiological processes in osteoblasts under mechanical stimuli 剪接因子调节机械生长因子的产生调节成骨细胞在机械刺激下的生理过程

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2019-01-01 DOI: 10.1080/19336918.2019.1686103

Qian Yi, Huan Liu, Jianguo Feng, Yanjiao Wu, Weichao Sun, Mengting Ou, Liling Tang

引用次数: 4

Paracrine signalling of AGR2 stimulates RhoA function in fibroblasts and modulates cell elongation and migration AGR2的旁分泌信号传导刺激成纤维细胞中的RhoA功能并调节细胞延伸和迁移

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2019-01-01 DOI: 10.1080/19336918.2019.1685928

H. B. Mangukiya, H. Negi, S. B. Merugu, Qudsia Sehar, D. S. Mashausi, F. Yunus, Zhenghua Wu, Dawei Li

引用次数: 4

Predictable fibroblast tension generation by measuring compaction of anchored collagen matrices using microscopy and optical coherence tomography 通过使用显微镜和光学相干断层扫描测量锚定胶原基质的压实，可预测成纤维细胞张力的产生

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2019-01-01 DOI: 10.1080/19336918.2019.1644855

Melville B Vaughan, Gang Xu, Tracy L. Morris, Pratiksha Kshetri, Jing X Herwig

{"title":"Predictable fibroblast tension generation by measuring compaction of anchored collagen matrices using microscopy and optical coherence tomography","authors":"Melville B Vaughan, Gang Xu, Tracy L. Morris, Pratiksha Kshetri, Jing X Herwig","doi":"10.1080/19336918.2019.1644855","DOIUrl":"https://doi.org/10.1080/19336918.2019.1644855","url":null,"abstract":"ABSTRACT The anchored fibroblast-populated collagen matrix (aFPCM) is an appropriate model to study fibrocontractive disease mechanisms. Our goal was to determine if aFPCM height reduction (compaction) during development is sufficient to predict tension generation. Compaction was quantified daily by both traditional light microscopy and an optical coherence tomography (OCT) system. Contraction in aFPCM was revealed by releasing them from anchorage. We found that aFPCM contraction increase was correlated to the compaction increase. Cytochalasin D treatment reversibly inhibited compaction. Therefore, we demonstrated that aFPCM height reduction efficiently measures compaction, contraction, and relative maturity of the collagen matrix during development or treatment. In addition, we showed that OCT is suitable for effectively imaging the cross-sectional morphology of the aFPCM in culture. This study will pave the way for more efficient studies on the mechanisms of (and treatments that target) migration and contraction in wound healing and Dupuytren’s contracture in a tissue environment.","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336918.2019.1644855","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45978409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Mathematical Models of Specific Cell Adhesion Phenomena 特定细胞粘附现象的数学模型

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2018-10-03 DOI: 10.1201/9781315138954-2

M. Hjortso, J. Roos

引用次数: 0

Kinetics of Ligand-Receptor Bond Formation 配体-受体键形成动力学

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2018-10-03 DOI: 10.1201/9781315138954-1

J. Roos, M. Hjortso

引用次数: 0

Adhesion structures in leukemia cells and their regulation by Src family kinases. 白血病细胞的粘附结构及其Src家族激酶的调控。

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2018-05-04 Epub Date: 2017-08-18 DOI: 10.1080/19336918.2017.1344796

Pavla Röselová, Adam Obr, Aleš Holoubek, Dana Grebeňová, Kateřina Kuželová

{"title":"Adhesion structures in leukemia cells and their regulation by Src family kinases.","authors":"Pavla Röselová, Adam Obr, Aleš Holoubek, Dana Grebeňová, Kateřina Kuželová","doi":"10.1080/19336918.2017.1344796","DOIUrl":"https://doi.org/10.1080/19336918.2017.1344796","url":null,"abstract":"Interaction of leukemia blasts with the bone marrow extracellular matrix often results in protection of leukemia cells from chemotherapy and in persistence of the residual disease which is on the basis of subsequent relapses. The adhesion signaling pathways have been extensively studied in adherent cells as well as in mature haematopoietic cells, but the adhesion structures and signaling in haematopoietic stem and progenitor cells, either normal or malignant, are much less explored. We analyzed the interaction of leukemia cells with fibronectin (FN) using interference reflection microscopy, immunofluorescence, measurement of adherent cell fraction, real-time microimpedance measurement and live cell imaging. We found that leukemia cells form very dynamic adhesion structures similar to early stages of focal adhesions. In contrast to adherent cells, where Src family kinases (SFK) belong to important regulators of focal adhesion dynamics, we observed only minor effects of SFK inhibitor dasatinib on leukemia cell binding to FN. The relatively weak involvement of SFK in adhesion structure regulation might be associated with the lack of cytoskeletal mechanical tension in leukemia cells. On the other hand, active Lyn kinase was found to specifically localize to leukemia cell adhesion structures and a less firm cell attachment to FN was often associated with higher Lyn activity (this unexpectedly occurred also after cell treatment with the inhibitor SKI-1). Lyn thus may be important for signaling from integrin-associated complexes to other processes in leukemia cells.","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2018-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336918.2017.1344796","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35143574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration. 复合3D打印支架与结构静电纺纳米纤维促进软骨细胞粘附和浸润。

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2018-05-04 Epub Date: 2017-11-13 DOI: 10.1080/19336918.2017.1385713

M Rampichová, E Košt'áková Kuželová, E Filová, J Chvojka, J Šafka, M Pelcl, J Daňková, E Prosecká, M Buzgo, M Plencner, D Lukáš, E Amler

{"title":"Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration.","authors":"M Rampichová, E Košt'áková Kuželová, E Filová, J Chvojka, J Šafka, M Pelcl, J Daňková, E Prosecká, M Buzgo, M Plencner, D Lukáš, E Amler","doi":"10.1080/19336918.2017.1385713","DOIUrl":"https://doi.org/10.1080/19336918.2017.1385713","url":null,"abstract":"Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2018-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336918.2017.1385713","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35248371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Vascular endothelial growth factor-B: Impact on physiology and pathology. 血管内皮生长因子- b:对生理和病理的影响。

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2018-05-04 Epub Date: 2017-11-02 DOI: 10.1080/19336918.2017.1379634

Hongyu Zhu, Mingming Gao, Xiangdong Gao, Yue Tong

引用次数: 9

Calpain2 mediates Rab5-driven focal adhesion disassembly and cell migration. Calpain2介导rab5驱动的黏附脱落和细胞迁移。

IF 3.2 3区生物学

Cell Adhesion & Migration Pub Date : 2018-05-04 Epub Date: 2017-11-06 DOI: 10.1080/19336918.2017.1377388

Pablo A Mendoza, Patricio Silva, Jorge Díaz, Cecilia Arriagada, Jimena Canales, Oscar Cerda, Vicente A Torres

{"title":"Calpain2 mediates Rab5-driven focal adhesion disassembly and cell migration.","authors":"Pablo A Mendoza, Patricio Silva, Jorge Díaz, Cecilia Arriagada, Jimena Canales, Oscar Cerda, Vicente A Torres","doi":"10.1080/19336918.2017.1377388","DOIUrl":"https://doi.org/10.1080/19336918.2017.1377388","url":null,"abstract":"The early endosome protein Rab5 was recently shown to promote cell migration by enhancing focal adhesion disassembly through mechanisms that remain elusive. Focal adhesion disassembly is associated to proteolysis of talin, in a process that requires calpain2. Since calpain2 has been found at vesicles and endosomal compartments, we hypothesized that Rab5 stimulates calpain2 activity, leading to enhanced focal adhesion disassembly in migrating cells. We observed that calpain2 co-localizes with EEA1-positive early endosomes and co-immunoprecipitates with EEA1 and Rab5 in A549 lung carcinoma cells undergoing spreading, whereas Rab5 knock-down decreased the accumulation of calpain2 at early endosomal-enriched fractions. In addition, Rab5 silencing decreased calpain2 activity, as shown by cleavage of the fluorogenic substrate tBOC-LM-CMAC and the endogenous substrate talin. Accordingly, Rab5 promoted focal adhesion disassembly in a calpain2-dependent manner, as expression of GFP-Rab5 accelerated focal adhesion disassembly in nocodazole-synchronized cells, whereas pharmacological inhibition of calpain2 with N-acetyl-Leu-Leu-Met prevented both focal adhesion disassembly and cell migration induced by Rab5. In summary, these data uncover Rab5 as a novel regulator of calpain2 activity and focal adhesion proteolysis leading to cell migration.","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2018-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336918.2017.1377388","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35518804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8